                         SEQUENCE LISTING

<110>  Beam Therapeutics Inc.
 
<120>  SYNTHETIC GUIDE RNA, COMPOSITIONS, METHODS, AND USES THEREOF

<130>  BEM-002WO

<140>  PCT/US20/63084
<141>  2020-12-03

<150>  63/031,262
<151>  2020-05-28

<150>  62/943,158
<151>  2019-12-03

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  14
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  1
cgauacgaca gaac                                                         14


<210>  2
<211>  5
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  2
cgccg                                                                    5


<210>  3
<211>  7
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  3
cggccgc                                                                  7


<210>  4
<211>  5
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  4
cgcgc                                                                    5


<210>  5
<211>  4
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  5
cgau                                                                     4


<210>  6
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  6
aucgacaaga aagggacuga guuuuagagc uaugcugu                               38


<210>  7
<211>  82
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (79)..(81)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (79)..(81)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  7
cuuggaaaca agacagcaua gcaaguuaaa auaaggcuag uccguuauca acuugaaaaa       60

guggcaccga gucggugcuu uu                                                82


<210>  8
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  8
aucgacaaga aagggacuga guuuuagagc uaugcugucu ugcucga                     47


<210>  9
<211>  76
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (73)..(75)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (73)..(75)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  9
uacaagacag cauagcaagu uaaaauaagg cuaguccguu aucaacuuga aaaaguggca       60

ccgagucggu gcuuuu                                                       76


<210>  10
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  10
aucgacaaga aagggacuga gcccuagagc g                                      31


<210>  11
<211>  71
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (68)..(70)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (68)..(70)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  11
gcgaaagccg caaguuaggg uaaggcuagu ccguuaucaa cuugaaaaag ugggaccgag       60

ucggugcuuu u                                                            71


<210>  12
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  12
aucgacaaga aagggacuga gcccuagagc cg                                     32


<210>  13
<211>  74
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (71)..(73)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (71)..(73)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  13
gcggaaacgc cggcaaguua ggguaaggcu aguccguuau caacuugaaa aagugggacc       60

gagucggugc uuuu                                                         74


<210>  14
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  14
aucgacaaga aagggacuga guuuuagagc cg                                     32


<210>  15
<211>  74
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (71)..(73)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (71)..(73)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  15
gcggaaacgc cggcaaguua aaauaaggcu aguccguuau caacuugaaa aagugggacc       60

gagucggugc uuuu                                                         74


<210>  16
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  16
aucgacaaga aagggacuga guuuuagagc                                        30


<210>  17
<211>  72
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (69)..(71)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (69)..(71)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  17
gcggaaacgc gcaaguuaaa auaaggcuag uccguuauca acuugaaaaa gugggaccga       60

gucggugcuu uu                                                           72


<210>  18
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  18
aucgacaaga aagggacuga guuuuagagc                                        30


<210>  19
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (67)..(69)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (67)..(69)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  19
uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu gggaccgagu       60

cggugcuuuu                                                              70


<210>  20
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  20
uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu gggaccgagu       60

cggugcuuuu                                                              70


<210>  21
<211>  72
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (41)..(72)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (69)..(71)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  21
gcggaaacgc gcaaguuaaa auaaggcuag uccguuauca acuugaaaaa guggcaccga       60

gucggugcuu uu                                                           72


<210>  22
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with 2'OMe modification

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  nucleotide with a 3' phosphorothioate modification

<400>  22
gaacacaaag cauagacugc guuuuagagc cg                                     32


