                         SEQUENCE LISTING

<110>  Arc Bio LLC
       FORST, Jannine
       RASMUSSEN, Morten
       GOURGUECHON, Stephane B
 
<120>  LIGATION FREE METHODS OF NUCLEIC ACID LIBRARY PREPARATION

<130>  155949.00090

<150>  62/945,051
<151>  2019-12-06

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer


<220>
<221>  misc_feature
<222>  (33)..(41)
<223>  n is a, c, g, or t

<400>  1
acactctttc cctacacgac gctcttccga tcnnnnnnnn n                           41


<210>  2
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  2
gactggagtt cagacgtgtg ctcttccgat ccccccc                                37


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence

<400>  3
gactggagtt cagacgtgtg ctcttccgat ct                                     32


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence

<400>  4
acactctttc cctacacgac gctcttccga tct                                    33


<210>  5
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer


<220>
<221>  misc_feature
<222>  (33)..(41)
<223>  n is a, c, g, or t

<400>  5
acactctttc cctacacgac gctcttccga tcnnnnnnnn n                           41


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence

<400>  6
gttttagagc tatgctgttt tg                                                22


<210>  7
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  encodes tracrRNA

<400>  7
ggaaccattc aaaacagcat agcaagttaa aataaggcta gtccgttatc aacttgaaaa       60

agtggcaccg agtcggtgct tttttt                                            86


<210>  8
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence on one strand

<400>  8
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt ttt                                               83


<210>  9
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse complement of gNA strand sequence

<400>  9
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aac                                               83


<210>  10
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence

<400>  10
aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa       60

cttgctattt ctagctctaa aac                                               83


<210>  11
<211>  83
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop sequence

<400>  11
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu uuu                                               83


<210>  12
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop

<400>  12
gttttagagc tatgctggaa acagcatagc aagttaaaat aaggctagtc cgttatcaac       60

ttgaaaaagt ggcaccgagt cggtgctttt tttc                                   94


<210>  13
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop reverse complement

<400>  13
gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta       60

acttgctatg ctgtttccag catagctcta aaac                                   94


<210>  14
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop

<400>  14
gaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta       60

acttgctatg ctgtttccag catagctcta aaac                                   94


<210>  15
<211>  94
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gNA stem-loop

<400>  15
guuuuagagc uaugcuggaa acagcauagc aaguuaaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugcuuuu uuuc                                   94


<210>  16
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gNA sequence

<400>  16
aauuucuacu guuguagau                                                    19


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gNA

<400>  17
aatttctact gttgtagat                                                    19


<210>  18
<211>  83
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  18
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu uuu                                               83


<210>  19
<211>  94
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  19
guuuuagagc uaugcuggaa acagcauagc aaguuaaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugcuuuu uuuc                                   94


<210>  20
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  20
guuuuagagc uaugcuguuu ug                                                22


<210>  21
<211>  86
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  21
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugcu uuuuuu                                            86


<210>  22
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA stem-loop

<400>  22
aauuucuacu guuguagau                                                    19


<210>  23
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 gRNA stem-loop

<400>  23
aauuucuacu guuguagau                                                    19


<210>  24
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence (Ad2)

<400>  24
gactggagtt cagacgtgtg ctcttccgat ccccccc                                37


<210>  25
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ad2 sequence with phosphorothioates

<400>  25
gactggagtt cagacgtgtg ctcttccgat ccccccc                                37


<210>  26
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adapter sequence (Ad1)


<220>
<221>  misc_feature
<222>  (33)..(41)
<223>  n is a, c, g, or t

<400>  26
acactctttc cctacacgac gctcttccga tcnnnnnnnn n                           41


<210>  27
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ad1 sequence with phosphorothioates


<220>
<221>  misc_feature
<222>  (33)..(41)
<223>  n is a, c, g, or t

<400>  27
acactctttc cctacacgac gctcttccga tcnnnnnnnn n                           41


