               SEQUENCE LISTING

<110> Synbionik GmbH

<120> An Efficient Shuttle Vector System for the expression of heterologous and homologous proteins for the genus Zymomonas 

<130> S75343WO

<150>  19213657.0
<151>  2019-12-04

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 2589
<212> DNA
<213> Synthetic plasmid


<220> 
<223> pZM-DUET

<400> 1
ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa      60

aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt     120

ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca     180

gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag     240

ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc     300

ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca     360

gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt     420

aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct     480

gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt     540

aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga     600

caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact     660

tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc     720

acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga     780

gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt     840

agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga     900

gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact     960

ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga    1020

taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt agaaaagatc    1080

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa    1140

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag    1200

gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta    1260

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta    1320

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag    1380

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg    1440

gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg    1500

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag    1560

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc    1620

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa    1680

aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcagcgg    1740

ccgcgatcta agaattcttc aaggtgtccc gttccttttt cccctttttg gaggttggtt    1800

atgtcctata atcacttaat ccagaaacgg gcgtttagct ttgtccatca tggttgttta    1860

tcgctcatga tcgcggcatg ttctgatatt tttcctctaa aaaagataaa aagtcttttc    1920

gcttcggcag aagaggttca tcatgaacaa aaattcggca tttttaaaaa tgcctatagc    1980

taaatccgga acgacacttt agaggtttct gggtcatcct gattcagaca tagtgttttg    2040

aatatatgga gtaagcagga tccgatctac catgggagcg agaaggaggt aaagtaagct    2100

tgatctactg cagtattatg aactgaattt aagaggctgc cttccgataa aatcgggagg    2160

tggccttttt tatatcaaca acccgaatcc tatcgtaatg atgttttgcc cgatcagcct    2220

caatcgacaa ttttacgcgt ttcgatcgaa gcagggacga caattggctg ggaacggtat    2280

actggaataa atggtcttcg ttatggtatt gatgtttttg gtgcatcggc cccggcgaat    2340

gatctatatg ctcatttcgg cttgaccgca gtcggcatca cgaacaaggt gttggccgcg    2400

atcgccggta agtcggcacg ttaaaaaata gctatggaat atagtagcta cttaataagt    2460

taggagaata aaccatatgg atctaagatc tgagcgagaa ggaggtaaag tggtaccgat    2520

ctactcgagt attatgaact gaatttaaga ggctgccttc cgataaaatc gggaggtggc    2580

cttttttat                                                            2589


<210> 2
<211> 36
<212> DNA
<213> Primer


<220> 
<223> AmpR_KanaR_CamR_SaII_FW

<400> 2
gcgcgtcgac ttcaaatatg tatccgctca tgagac                               36


<210> 3
<211> 32
<212> DNA
<213> Primer


<220> 
<223> AmpR_KanaR_CamR_Xbal_RV

<400> 3
gcgctctaga gctcagtgga acgaaaactc ac                                   32


<210> 4
<211> 33
<212> DNA
<213> Primer


<220> 
<223> TetR_Xmal_FW

<400> 4
gctacccggg tcatgtttga cagcttatca tcg                                  33


<210> 5
<211> 32
<212> DNA
<213> Primer


<220> 
<223> TetR_Xbal_RV

<400> 5
gcgctctaga ggatatgttc tgccaagggt tg                                   32


<210> 6
<211> 38
<212> DNA
<213> Primer


<220> 
<223> SpecR_SaII_FW

<400> 6
gcgcgtcgac aaattctcat gtttgacagc ttatcatc                             38


<210> 7
<211> 30
<212> DNA
<213> Primer


<220> 
<223> SpecR_Xbal_RV

<400> 7
gcgctctaga cgcatagtct tccccagctc                                     30


<210> 8
<211> 60
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> ZM_Terminator

<400> 8
tattatgaac tgaatttaag aggctgcctt ccgataaaat cgggaggtgg ccttttttat     60


<210> 9
<211> 20
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Ribosome-binding site 1

<400> 9
gagcgagaag gaggtaaagt                                                20


<210> 10
<211> 26
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Ribosome-binding site 2

<400> 10
atcacagggt ctagaaggag gtcgaa                                         26


<210> 11
<211> 27
<212> DNA
<213> Zymomonas mobilis


<220> 
<223> Ribosome-binding site 3

<400> 11
aggctaagaa ctaacggaga ggtaaat                                        27


<210> 12
<211> 35
<212> DNA
<213> Primer


<220> 
<223> pZMDuet_colE1_Xbal_FW

<400> 12
gcgctctaga cactgagcgt agaaaagatc aaagg                                35


<210> 13
<211> 34
<212> DNA
<213> Primer


<220> 
<223> pZMDuet_AmpR_Sall_RV

<400> 13
gcgcgtcgac gtctcatgag cggatacata tttg                                 34


<210> 14
<211> 34
<212> DNA
<213> Primer


<220> 
<223> pZMDuet_AmpR_Xmal_RV

<400> 14
gcgccccggg gtctcatgag cggatacata tttg                                 34


