               SEQUENCE LISTING

<110> Synbionik Gmbh

<120> Non-naturally occurring bacteria modified able to produce tryptophan derived compounds (DMT)

<130> S75342WO

<150> 19213656.2
<151> 2019-12-04

<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 1503
<212> DNA
<213> Carathanthus roseus


<220> 
<223> Tryptophan Decarboxylase (Aromatic Amino acid Decarboxylase,
      AADC)

<400> 1
atgggcagca ttgatagcac caacgtggcg atgagcaaca gcccggtggg cgaatttaaa      60

ccgctggaag cggaagaatt tcgcaaacag gcgcatcgca tggtggattt tattgcggat     120

tattataaaa acgtggaaac ctatccggtg ctgagcgaag tggaaccggg ctatctgcgc     180

aaacgcattc cggaaaccgc gccgtatctg ccggaaccgc tggatgatat tatgaaagat     240

attcagaaag atattattcc gggcatgacc aactggatga gcccgaactt ttatgcgttt     300

tttccggcga ccgtgagcag cgcggcgttt ctgggcgaaa tgctgagcac cgcgctgaac     360

agcgtgggct ttacctgggt gagcagcccg gcggcgaccg aactggaaat gattgtgatg     420

gattggctgg cgcagattct gaaactgccg aaaagtttca tgtttagcgg caccggcggc     480

ggcgtgattc agaacaccac cagcgaaagc attctgtgca ccattattgc ggcgcgcgaa     540

cgcgcgctgg aaaaactggg cccggatagc attggcaaac tggtgtgcta tggcagcgat     600

cagacccata ccatgtttcc gaaaacctgc aaactggcgg gcatttatcc gaacaacatt     660

cgcctgattc cgaccaccgt ggaaaccgat tttggcatta gcccgcaggt gctgcgcaaa     720

atggtggaag atgatgtggc ggcgggctat gtgccgctgt ttctgtgcgc gaccctgggc     780

accaccagca ccaccgcgac cgatccggtg gatagcctga gcgaaattgc gaacgaattt     840

ggcatttgga ttcatgtgga tgcggcgtat gcgggcagcg cgtgcatttg cccggaattt     900

cgccattatc tggatggcat tgaacgcgtg gatagcctga gcctgagccc gcataaatgg     960

ctgctggcgt atctggattg cacctgcctg tgggtgaaac agccgcatct gctgctgcgc    1020

gcgctgacca ccaacccgga atatctgaag aacaaacaga gcgatctgga taaagtggtg    1080

gattttaaaa actggcagat tgcgaccggc cgcaaatttc gcagcctgaa actgtggctg    1140

attctgcgca gctatggcgt ggtgaacctt cagagccata ttcgcagcga tgtggcgatg    1200

ggcaaaatgt ttgaagaatg ggtgcgcagc gatagccgct ttgaaattgt ggtgccgcgc    1260

aactttagcc tggtgtgctt tcgcctgaaa ccggatgtga gcagcctgca tgtggaagaa    1320

gtgaacaaga aactgctgga tatgctgaac agcaccggcc gcgtgtatat gacccatacc    1380

attgtgggcg gcatttatat gctgcgcctg gcggtgggca gcagcctgac cgaagaacat    1440

catgtgcgcc gcgtgtggga tctgattcag aaactgaccg atgatctgct gaaagaagcg    1500

taa                                                                  1503


<210> 2
<211> 500
<212> PRT
<213> Carathanthus roseus


<220> 
<223> Aromatic Amino acid decarboxylase enzyme

<400> 2
Met Gly Ser Ile Asp Ser Thr Asn Val Ala Met Ser Asn Ser Pro Val 
1               5                   10                  15      
Gly Glu Phe Lys Pro Leu Glu Ala Glu Glu Phe Arg Lys Gln Ala His 
            20                  25                  30          
Arg Met Val Asp Phe Ile Ala Asp Tyr Tyr Lys Asn Val Glu Thr Tyr 
        35                  40                  45              
Pro Val Leu Ser Glu Val Glu Pro Gly Tyr Leu Arg Lys Arg Ile Pro 
    50                  55                  60                  
Glu Thr Ala Pro Tyr Leu Pro Glu Pro Leu Asp Asp Ile Met Lys Asp 
65                  70                  75                  80  
Ile Gln Lys Asp Ile Ile Pro Gly Met Thr Asn Trp Met Ser Pro Asn 
                85                  90                  95      
Phe Tyr Ala Phe Phe Pro Ala Thr Val Ser Ser Ala Ala Phe Leu Gly 
            100                 105                 110         
Glu Met Leu Ser Thr Ala Leu Asn Ser Val Gly Phe Thr Trp Val Ser 
        115                 120                 125             
Ser Pro Ala Ala Thr Glu Leu Glu Met Ile Val Met Asp Trp Leu Ala 
    130                 135                 140                 
Gln Ile Leu Lys Leu Pro Lys Ser Phe Met Phe Ser Gly Thr Gly Gly 
145                 150                 155                 160 
Gly Val Ile Gln Asn Thr Thr Ser Glu Ser Ile Leu Cys Thr Ile Ile 
                165                 170                 175     
Ala Ala Arg Glu Arg Ala Leu Glu Lys Leu Gly Pro Asp Ser Ile Gly 
            180                 185                 190         
Lys Leu Val Cys Tyr Gly Ser Asp Gln Thr His Thr Met Phe Pro Lys 
        195                 200                 205             
Thr Cys Lys Leu Ala Gly Ile Tyr Pro Asn Asn Ile Arg Leu Ile Pro 
    210                 215                 220                 
Thr Thr Val Glu Thr Asp Phe Gly Ile Ser Pro Gln Val Leu Arg Lys 
225                 230                 235                 240 
Met Val Glu Asp Asp Val Ala Ala Gly Tyr Val Pro Leu Phe Leu Cys 
                245                 250                 255     
Ala Thr Leu Gly Thr Thr Ser Thr Thr Ala Thr Asp Pro Val Asp Ser 
            260                 265                 270         
Leu Ser Glu Ile Ala Asn Glu Phe Gly Ile Trp Ile His Val Asp Ala 
        275                 280                 285             
Ala Tyr Ala Gly Ser Ala Cys Ile Cys Pro Glu Phe Arg His Tyr Leu 
    290                 295                 300                 
Asp Gly Ile Glu Arg Val Asp Ser Leu Ser Leu Ser Pro His Lys Trp 
305                 310                 315                 320 
Leu Leu Ala Tyr Leu Asp Cys Thr Cys Leu Trp Val Lys Gln Pro His 
                325                 330                 335     
Leu Leu Leu Arg Ala Leu Thr Thr Asn Pro Glu Tyr Leu Lys Asn Lys 
            340                 345                 350         
Gln Ser Asp Leu Asp Lys Val Val Asp Phe Lys Asn Trp Gln Ile Ala 
        355                 360                 365             
Thr Gly Arg Lys Phe Arg Ser Leu Lys Leu Trp Leu Ile Leu Arg Ser 
    370                 375                 380                 
Tyr Gly Val Val Asn Leu Gln Ser His Ile Arg Ser Asp Val Ala Met 
385                 390                 395                 400 
Gly Lys Met Phe Glu Glu Trp Val Arg Ser Asp Ser Arg Phe Glu Ile 
                405                 410                 415     
Val Val Pro Arg Asn Phe Ser Leu Val Cys Phe Arg Leu Lys Pro Asp 
            420                 425                 430         
Val Ser Ser Leu His Val Glu Glu Val Asn Lys Lys Leu Leu Asp Met 
        435                 440                 445             
Leu Asn Ser Thr Gly Arg Val Tyr Met Thr His Thr Ile Val Gly Gly 
    450                 455                 460                 
Ile Tyr Met Leu Arg Leu Ala Val Gly Ser Ser Leu Thr Glu Glu His 
465                 470                 475                 480 
His Val Arg Arg Val Trp Asp Leu Ile Gln Lys Leu Thr Asp Asp Leu 
                485                 490                 495     
Leu Lys Glu Ala 
            500 

<210> 3
<211> 792
<212> DNA
<213> Homo sapiens


<220> 
<223> Indolethylamine N-methyltransferase (INMT) (EC Nr.: 2.1.1.49
      (formerly EC Nr.: 2.1.1.81))

<400> 3
atgaaaggcg gctttaccgg cggcgatgaa tatcagaaac attttctgcc gcgcgattat      60

ctggcgacct attatagctt tgatggcagc ccgagcccgg aagcggaaat gctgaaattt     120

aacctggaat gcctgcataa aacctttggc cctggcggcc ttcagggcga taccctgatt     180

gatattggca gcggcccgac catttatcag gtgctggcgg cgtgcgatag ctttcaggat     240

attaccctga gcgattttac cgatcgcaac cgcgaagaac tggaaaaatg gctgaagaaa     300

gaaccgggcg cgtatgattg gaccccggcg gtgaaatttg cgtgcgaact ggaaggcaac     360

agcggtcgct gggaagaaaa agaagaaaaa ctgcgcgcgg cggtgaaacg cgtgctgaaa     420

tgcgatgtgc atctgggcaa cccgctggcg cctgcggtgc tgccgctggc ggattgcgtg     480

ctgaccctgc tggcgatgga atgcgcgtgc tgtagcctgg atgcgtatcg cgcggcgctg     540

tgcaacctgg cgagcctgct gaaaccgggc ggccatctgg tgaccaccgt gaccctgcgc     600

ctgccgagct atatggtggg caaacgcgaa tttagctgcg tggcgctgga aaaagaagaa     660

gtggaacagg cggtgctgga tgcgggcttt gatattgaac agctgctgca tagcccgcag     720

agctatagcg tgaccaacgc ggcgaacaac ggcgtgtgct ttattgtggc gcgcaagaaa     780

ccgggcccgt aa                                                         792


<210> 4
<211> 263
<212> PRT
<213> Homo sapiens


<220> 
<223> Indolethylamine N-methyltransferase (INMT) (EC Nr.: 2.1.1.49
      (formerly EC Nr.: 2.1.1.81))

<400> 4
Met Lys Gly Gly Phe Thr Gly Gly Asp Glu Tyr Gln Lys His Phe Leu 
1               5                   10                  15      
Pro Arg Asp Tyr Leu Ala Thr Tyr Tyr Ser Phe Asp Gly Ser Pro Ser 
            20                  25                  30          
Pro Glu Ala Glu Met Leu Lys Phe Asn Leu Glu Cys Leu His Lys Thr 
        35                  40                  45              
Phe Gly Pro Gly Gly Leu Gln Gly Asp Thr Leu Ile Asp Ile Gly Ser 
    50                  55                  60                  
Gly Pro Thr Ile Tyr Gln Val Leu Ala Ala Cys Asp Ser Phe Gln Asp 
65                  70                  75                  80  
Ile Thr Leu Ser Asp Phe Thr Asp Arg Asn Arg Glu Glu Leu Glu Lys 
                85                  90                  95      
Trp Leu Lys Lys Glu Pro Gly Ala Tyr Asp Trp Thr Pro Ala Val Lys 
            100                 105                 110         
Phe Ala Cys Glu Leu Glu Gly Asn Ser Gly Arg Trp Glu Glu Lys Glu 
        115                 120                 125             
Glu Lys Leu Arg Ala Ala Val Lys Arg Val Leu Lys Cys Asp Val His 
    130                 135                 140                 
Leu Gly Asn Pro Leu Ala Pro Ala Val Leu Pro Leu Ala Asp Cys Val 
145                 150                 155                 160 
Leu Thr Leu Leu Ala Met Glu Cys Ala Cys Cys Ser Leu Asp Ala Tyr 
                165                 170                 175     
Arg Ala Ala Leu Cys Asn Leu Ala Ser Leu Leu Lys Pro Gly Gly His 
            180                 185                 190         
Leu Val Thr Thr Val Thr Leu Arg Leu Pro Ser Tyr Met Val Gly Lys 
        195                 200                 205             
Arg Glu Phe Ser Cys Val Ala Leu Glu Lys Glu Glu Val Glu Gln Ala 
    210                 215                 220                 
Val Leu Asp Ala Gly Phe Asp Ile Glu Gln Leu Leu His Ser Pro Gln 
225                 230                 235                 240 
Ser Tyr Ser Val Thr Asn Ala Ala Asn Asn Gly Val Cys Phe Ile Val 
                245                 250                 255     
Ala Arg Lys Lys Pro Gly Pro 
            260             

