               SEQUENCE LISTING

<110> FrieslandCampina Nederland B.V.

<120> Method for preparing galacto-oligosaccharides from lactulose
<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 696
<212> PRT
<213> Cryptococcus terrestris


<400> 1
Met Ile Pro Ala Ser Ala Leu Leu Ala Ala Val Pro Leu Leu Ala Gln 
1               5                   10                  15      
Gln Val Ser Ala Gly Ile Leu Arg Arg Gln Asn Ala Ala Gly Ser Asp 
            20                  25                  30          
Ser Ala Ala Pro Asp Ser Ile Ala Asp Ala Ser Thr Gly Val Val Ser 
        35                  40                  45              
Ser Ile Ala Thr Glu Ala Val Ser Ser Gly Ala Thr Gly Leu Val Ala 
    50                  55                  60                  
Ser Val Ala Met Ser Phe Ala Ser Ser Met Ala Thr Pro Thr Ala Thr 
65                  70                  75                  80  
Val Thr Gly Leu Ser Ser Glu Thr Gly Ala Pro Ser Asn Thr Pro Met 
                85                  90                  95      
Ala Ser Ala Ser Gly Ser Val Pro Thr Thr Thr Ser Ala Val Gly Ser 
            100                 105                 110         
Gly Asp Phe Asp Trp Val Gln Thr Asp Gly Leu Pro Thr Ile Thr Thr 
        115                 120                 125             
Thr Leu Ala Thr Thr Asn Gln Asp Ala Ile Thr Pro Thr Ala Thr Gly 
    130                 135                 140                 
Pro Val Gly Gly Gln Gly Thr Pro Ala Val Asn Phe Thr Asp Tyr Ser 
145                 150                 155                 160 
Ser Ser Ser Leu Glu Gln Phe Trp Asn Asp Trp Val Gly Glu Val Glu 
                165                 170                 175     
Glu Pro Pro Phe Ala Tyr Val Pro Glu Pro Pro Asn Pro Tyr Pro Leu 
            180                 185                 190         
Pro Asn Ala Pro Pro Pro Ile Tyr Pro Glu Tyr Tyr Thr Lys Arg Pro 
        195                 200                 205             
Lys Asp Ile Leu Pro Asp Tyr Lys Phe Pro Lys Asp Phe Leu Phe Gly 
    210                 215                 220                 
Trp Ala Thr Ala Ala Gln Gln Trp Glu Gly Ala Val Lys Ala Asp Gly 
225                 230                 235                 240 
Lys Gly Pro Ser Ile Trp Asp Trp Ala Ser Arg Phe Pro Gly Phe Ile 
                245                 250                 255     
Ala Asp Asn Thr Thr Ser Asp Val Gly Asp Leu Gly Tyr Tyr Leu Tyr 
            260                 265                 270         
Lys Glu Asp Leu Ala Arg Ile Ala Ala Leu Gly Ala Asn Val Tyr Ser 
        275                 280                 285             
Phe Ser Met Phe Trp Thr Arg Ile Phe Pro Phe Gly Lys Ala Asp Ser 
    290                 295                 300                 
Pro Val Asn Gln Ala Gly Ile Asp Phe Tyr His Asp Leu Ile Asp Tyr 
305                 310                 315                 320 
Ser Trp Ser Leu Gly Ile Glu Pro Val Val Thr Leu Phe His Trp Asp 
                325                 330                 335     
Thr Pro Leu Ala Leu Gln Leu Glu Tyr Gly Gly Phe Ala Ser Glu Arg 
            340                 345                 350         
Ile Ile Asp Asp Tyr Val Asn Tyr Ala Glu Thr Val Phe Lys Ala Tyr 
        355                 360                 365             
Asn Gly Ser Val His Lys Trp Val Thr Phe Asn Glu Pro Val Val Phe 
    370                 375                 380                 
Cys Ser Gln Met Ala Ala Pro Val Asn Thr Thr Leu Pro Pro Asn Leu 
385                 390                 395                 400 
Asn Ser Thr Ile Tyr Pro Tyr Thr Cys Ser Tyr His Leu Val Leu Ala 
                405                 410                 415     
His Ala Lys Thr Val Lys Arg Phe Arg Glu Leu Asn Ile Gln Gly Gln 
            420                 425                 430         
Ile Ala Phe Lys Ser Asp Asn Phe Val Gly Ile Pro Trp Arg Glu Gly 
        435                 440                 445             
Asn Gln Glu Asp Ile Asp Ala Val Glu Arg His Gln Ala Tyr Gln Ile 
    450                 455                 460                 
Gly Ile Phe Ala Glu Pro Ile Tyr Asn Thr Gly Asp Trp Pro Asp Ile 
465                 470                 475                 480 
Val Lys Asn Asp Leu Ser Pro Asp Ile Leu Pro Arg Phe Thr Asp Asp 
                485                 490                 495     
Glu Ile Ala Met Ile Lys Cys Thr Ala Asp Phe Phe Pro Ile Asp Gly 
            500                 505                 510         
Tyr Arg Asp Gly Tyr Val Gln Ala Val Pro Gly Gly Val Glu Ala Cys 
        515                 520                 525             
Val Ala Asn Ile Ser Asn Pro Leu Trp Pro Ala Cys Asn Gln Val Asn 
    530                 535                 540                 
Phe Tyr Asp Ser Thr Pro Ala Gly Trp Ala Ile Gly Thr Phe Gly Asn 
545                 550                 555                 560 
Trp Pro Thr Thr Pro Trp Leu Gln Asn Thr Trp Gln Phe Val Arg Pro 
                565                 570                 575     
Phe Leu Ala Asp Leu Ala Lys Arg Tyr Pro Thr Glu Gly Gly Ile Tyr 
            580                 585                 590         
Leu Ser Glu Phe Gly Phe Ser Glu Pro Phe Glu Asn Asp Lys Thr Phe 
        595                 600                 605             
Ile Tyr Gln Ile Thr Gln Asp Ser Gly Arg Thr Ala Tyr Phe Asn Ser 
    610                 615                 620                 
Tyr Leu Gly Glu Val Leu Lys Gly Ile Val Glu Asp Gly Ile Pro Ile 
625                 630                 635                 640 
Lys Gly Val Phe Gly Trp Ser Met Val Asp Asn Phe Glu Trp Asn Ser 
                645                 650                 655     
Gly Leu Ser Thr Arg Phe Gly Val Gln Tyr Val Asp Tyr Asn Ser Pro 
            660                 665                 670         
Thr Arg Gln Arg Thr Phe Lys Arg Ser Ala Leu Glu Met Ser Glu Phe 
        675                 680                 685             
Trp Asn Ala His Arg Cys Ser Ala 
    690                 695     

<210> 2
<211> 566
<212> PRT
<213> Cryptococcus terrestris


<400> 2
Ala Thr Thr Asn Gln Asp Ala Ile Thr Pro Thr Ala Thr Gly Pro Val 
1               5                   10                  15      
Gly Gly Gln Gly Thr Pro Ala Val Asn Phe Thr Asp Tyr Ser Ser Ser 
            20                  25                  30          
Ser Leu Glu Gln Phe Trp Asn Asp Trp Val Gly Glu Val Glu Glu Pro 
        35                  40                  45              
Pro Phe Ala Tyr Val Pro Glu Pro Pro Asn Pro Tyr Pro Leu Pro Asn 
    50                  55                  60                  
Ala Pro Pro Pro Ile Tyr Pro Glu Tyr Tyr Thr Lys Arg Pro Lys Asp 
65                  70                  75                  80  
Ile Leu Pro Asp Tyr Lys Phe Pro Lys Asp Phe Leu Phe Gly Trp Ala 
                85                  90                  95      
Thr Ala Ala Gln Gln Trp Glu Gly Ala Val Lys Ala Asp Gly Lys Gly 
            100                 105                 110         
Pro Ser Ile Trp Asp Trp Ala Ser Arg Phe Pro Gly Phe Ile Ala Asp 
        115                 120                 125             
Asn Thr Thr Ser Asp Val Gly Asp Leu Gly Tyr Tyr Leu Tyr Lys Glu 
    130                 135                 140                 
Asp Leu Ala Arg Ile Ala Ala Leu Gly Ala Asn Val Tyr Ser Phe Ser 
145                 150                 155                 160 
Met Phe Trp Thr Arg Ile Phe Pro Phe Gly Lys Ala Asp Ser Pro Val 
                165                 170                 175     
Asn Gln Ala Gly Ile Asp Phe Tyr His Asp Leu Ile Asp Tyr Ser Trp 
            180                 185                 190         
Ser Leu Gly Ile Glu Pro Val Val Thr Leu Phe His Trp Asp Thr Pro 
        195                 200                 205             
Leu Ala Leu Gln Leu Glu Tyr Gly Gly Phe Ala Ser Glu Arg Ile Ile 
    210                 215                 220                 
Asp Asp Tyr Val Asn Tyr Ala Glu Thr Val Phe Lys Ala Tyr Asn Gly 
225                 230                 235                 240 
Ser Val His Lys Trp Val Thr Phe Asn Glu Pro Val Val Phe Cys Ser 
                245                 250                 255     
Gln Met Ala Ala Pro Val Asn Thr Thr Leu Pro Pro Asn Leu Asn Ser 
            260                 265                 270         
Thr Ile Tyr Pro Tyr Thr Cys Ser Tyr His Leu Val Leu Ala His Ala 
        275                 280                 285             
Lys Thr Val Lys Arg Phe Arg Glu Leu Asn Ile Gln Gly Gln Ile Ala 
    290                 295                 300                 
Phe Lys Ser Asp Asn Phe Val Gly Ile Pro Trp Arg Glu Gly Asn Gln 
305                 310                 315                 320 
Glu Asp Ile Asp Ala Val Glu Arg His Gln Ala Tyr Gln Ile Gly Ile 
                325                 330                 335     
Phe Ala Glu Pro Ile Tyr Asn Thr Gly Asp Trp Pro Asp Ile Val Lys 
            340                 345                 350         
Asn Asp Leu Ser Pro Asp Ile Leu Pro Arg Phe Thr Asp Asp Glu Ile 
        355                 360                 365             
Ala Met Ile Lys Cys Thr Ala Asp Phe Phe Pro Ile Asp Gly Tyr Arg 
    370                 375                 380                 
Asp Gly Tyr Val Gln Ala Val Pro Gly Gly Val Glu Ala Cys Val Ala 
385                 390                 395                 400 
Asn Ile Ser Asn Pro Leu Trp Pro Ala Cys Asn Gln Val Asn Phe Tyr 
                405                 410                 415     
Asp Ser Thr Pro Ala Gly Trp Ala Ile Gly Thr Phe Gly Asn Trp Pro 
            420                 425                 430         
Thr Thr Pro Trp Leu Gln Asn Thr Trp Gln Phe Val Arg Pro Phe Leu 
        435                 440                 445             
Ala Asp Leu Ala Lys Arg Tyr Pro Thr Glu Gly Gly Ile Tyr Leu Ser 
    450                 455                 460                 
Glu Phe Gly Phe Ser Glu Pro Phe Glu Asn Asp Lys Thr Phe Ile Tyr 
465                 470                 475                 480 
Gln Ile Thr Gln Asp Ser Gly Arg Thr Ala Tyr Phe Asn Ser Tyr Leu 
                485                 490                 495     
Gly Glu Val Leu Lys Gly Ile Val Glu Asp Gly Ile Pro Ile Lys Gly 
            500                 505                 510         
Val Phe Gly Trp Ser Met Val Asp Asn Phe Glu Trp Asn Ser Gly Leu 
        515                 520                 525             
Ser Thr Arg Phe Gly Val Gln Tyr Val Asp Tyr Asn Ser Pro Thr Arg 
    530                 535                 540                 
Gln Arg Thr Phe Lys Arg Ser Ala Leu Glu Met Ser Glu Phe Trp Asn 
545                 550                 555                 560 
Ala His Arg Cys Ser Ala 
                565     

<210> 3
<211> 560
<212> PRT
<213> Cryptococcus terrestris


<400> 3
Ala Ile Thr Pro Thr Ala Thr Gly Pro Val Gly Gly Gln Gly Thr Pro 
1               5                   10                  15      
Ala Val Asn Phe Thr Asp Tyr Ser Ser Ser Ser Leu Glu Gln Phe Trp 
            20                  25                  30          
Asn Asp Trp Val Gly Glu Val Glu Glu Pro Pro Phe Ala Tyr Val Pro 
        35                  40                  45              
Glu Pro Pro Asn Pro Tyr Pro Leu Pro Asn Ala Pro Pro Pro Ile Tyr 
    50                  55                  60                  
Pro Glu Tyr Tyr Thr Lys Arg Pro Lys Asp Ile Leu Pro Asp Tyr Lys 
65                  70                  75                  80  
Phe Pro Lys Asp Phe Leu Phe Gly Trp Ala Thr Ala Ala Gln Gln Trp 
                85                  90                  95      
Glu Gly Ala Val Lys Ala Asp Gly Lys Gly Pro Ser Ile Trp Asp Trp 
            100                 105                 110         
Ala Ser Arg Phe Pro Gly Phe Ile Ala Asp Asn Thr Thr Ser Asp Val 
        115                 120                 125             
Gly Asp Leu Gly Tyr Tyr Leu Tyr Lys Glu Asp Leu Ala Arg Ile Ala 
    130                 135                 140                 
Ala Leu Gly Ala Asn Val Tyr Ser Phe Ser Met Phe Trp Thr Arg Ile 
145                 150                 155                 160 
Phe Pro Phe Gly Lys Ala Asp Ser Pro Val Asn Gln Ala Gly Ile Asp 
                165                 170                 175     
Phe Tyr His Asp Leu Ile Asp Tyr Ser Trp Ser Leu Gly Ile Glu Pro 
            180                 185                 190         
Val Val Thr Leu Phe His Trp Asp Thr Pro Leu Ala Leu Gln Leu Glu 
        195                 200                 205             
Tyr Gly Gly Phe Ala Ser Glu Arg Ile Ile Asp Asp Tyr Val Asn Tyr 
    210                 215                 220                 
Ala Glu Thr Val Phe Lys Ala Tyr Asn Gly Ser Val His Lys Trp Val 
225                 230                 235                 240 
Thr Phe Asn Glu Pro Val Val Phe Cys Ser Gln Met Ala Ala Pro Val 
                245                 250                 255     
Asn Thr Thr Leu Pro Pro Asn Leu Asn Ser Thr Ile Tyr Pro Tyr Thr 
            260                 265                 270         
Cys Ser Tyr His Leu Val Leu Ala His Ala Lys Thr Val Lys Arg Phe 
        275                 280                 285             
Arg Glu Leu Asn Ile Gln Gly Gln Ile Ala Phe Lys Ser Asp Asn Phe 
    290                 295                 300                 
Val Gly Ile Pro Trp Arg Glu Gly Asn Gln Glu Asp Ile Asp Ala Val 
305                 310                 315                 320 
Glu Arg His Gln Ala Tyr Gln Ile Gly Ile Phe Ala Glu Pro Ile Tyr 
                325                 330                 335     
Asn Thr Gly Asp Trp Pro Asp Ile Val Lys Asn Asp Leu Ser Pro Asp 
            340                 345                 350         
Ile Leu Pro Arg Phe Thr Asp Asp Glu Ile Ala Met Ile Lys Cys Thr 
        355                 360                 365             
Ala Asp Phe Phe Pro Ile Asp Gly Tyr Arg Asp Gly Tyr Val Gln Ala 
    370                 375                 380                 
Val Pro Gly Gly Val Glu Ala Cys Val Ala Asn Ile Ser Asn Pro Leu 
385                 390                 395                 400 
Trp Pro Ala Cys Asn Gln Val Asn Phe Tyr Asp Ser Thr Pro Ala Gly 
                405                 410                 415     
Trp Ala Ile Gly Thr Phe Gly Asn Trp Pro Thr Thr Pro Trp Leu Gln 
            420                 425                 430         
Asn Thr Trp Gln Phe Val Arg Pro Phe Leu Ala Asp Leu Ala Lys Arg 
        435                 440                 445             
Tyr Pro Thr Glu Gly Gly Ile Tyr Leu Ser Glu Phe Gly Phe Ser Glu 
    450                 455                 460                 
Pro Phe Glu Asn Asp Lys Thr Phe Ile Tyr Gln Ile Thr Gln Asp Ser 
465                 470                 475                 480 
Gly Arg Thr Ala Tyr Phe Asn Ser Tyr Leu Gly Glu Val Leu Lys Gly 
                485                 490                 495     
Ile Val Glu Asp Gly Ile Pro Ile Lys Gly Val Phe Gly Trp Ser Met 
            500                 505                 510         
Val Asp Asn Phe Glu Trp Asn Ser Gly Leu Ser Thr Arg Phe Gly Val 
        515                 520                 525             
Gln Tyr Val Asp Tyr Asn Ser Pro Thr Arg Gln Arg Thr Phe Lys Arg 
    530                 535                 540                 
Ser Ala Leu Glu Met Ser Glu Phe Trp Asn Ala His Arg Cys Ser Ala 
545                 550                 555                 560 



<210> 4
<211> 555
<212> PRT
<213> Cryptococcus terrestris


<400> 4
Ala Thr Gly Pro Val Gly Gly Gln Gly Thr Pro Ala Val Asn Phe Thr 
1               5                   10                  15      
Asp Tyr Ser Ser Ser Ser Leu Glu Gln Phe Trp Asn Asp Trp Val Gly 
            20                  25                  30          
Glu Val Glu Glu Pro Pro Phe Ala Tyr Val Pro Glu Pro Pro Asn Pro 
        35                  40                  45              
Tyr Pro Leu Pro Asn Ala Pro Pro Pro Ile Tyr Pro Glu Tyr Tyr Thr 
    50                  55                  60                  
Lys Arg Pro Lys Asp Ile Leu Pro Asp Tyr Lys Phe Pro Lys Asp Phe 
65                  70                  75                  80  
Leu Phe Gly Trp Ala Thr Ala Ala Gln Gln Trp Glu Gly Ala Val Lys 
                85                  90                  95      
Ala Asp Gly Lys Gly Pro Ser Ile Trp Asp Trp Ala Ser Arg Phe Pro 
            100                 105                 110         
Gly Phe Ile Ala Asp Asn Thr Thr Ser Asp Val Gly Asp Leu Gly Tyr 
        115                 120                 125             
Tyr Leu Tyr Lys Glu Asp Leu Ala Arg Ile Ala Ala Leu Gly Ala Asn 
    130                 135                 140                 
Val Tyr Ser Phe Ser Met Phe Trp Thr Arg Ile Phe Pro Phe Gly Lys 
145                 150                 155                 160 
Ala Asp Ser Pro Val Asn Gln Ala Gly Ile Asp Phe Tyr His Asp Leu 
                165                 170                 175     
Ile Asp Tyr Ser Trp Ser Leu Gly Ile Glu Pro Val Val Thr Leu Phe 
            180                 185                 190         
His Trp Asp Thr Pro Leu Ala Leu Gln Leu Glu Tyr Gly Gly Phe Ala 
        195                 200                 205             
Ser Glu Arg Ile Ile Asp Asp Tyr Val Asn Tyr Ala Glu Thr Val Phe 
    210                 215                 220                 
Lys Ala Tyr Asn Gly Ser Val His Lys Trp Val Thr Phe Asn Glu Pro 
225                 230                 235                 240 
Val Val Phe Cys Ser Gln Met Ala Ala Pro Val Asn Thr Thr Leu Pro 
                245                 250                 255     
Pro Asn Leu Asn Ser Thr Ile Tyr Pro Tyr Thr Cys Ser Tyr His Leu 
            260                 265                 270         
Val Leu Ala His Ala Lys Thr Val Lys Arg Phe Arg Glu Leu Asn Ile 
        275                 280                 285             
Gln Gly Gln Ile Ala Phe Lys Ser Asp Asn Phe Val Gly Ile Pro Trp 
    290                 295                 300                 
Arg Glu Gly Asn Gln Glu Asp Ile Asp Ala Val Glu Arg His Gln Ala 
305                 310                 315                 320 
Tyr Gln Ile Gly Ile Phe Ala Glu Pro Ile Tyr Asn Thr Gly Asp Trp 
                325                 330                 335     
Pro Asp Ile Val Lys Asn Asp Leu Ser Pro Asp Ile Leu Pro Arg Phe 
            340                 345                 350         
Thr Asp Asp Glu Ile Ala Met Ile Lys Cys Thr Ala Asp Phe Phe Pro 
        355                 360                 365             
Ile Asp Gly Tyr Arg Asp Gly Tyr Val Gln Ala Val Pro Gly Gly Val 
    370                 375                 380                 
Glu Ala Cys Val Ala Asn Ile Ser Asn Pro Leu Trp Pro Ala Cys Asn 
385                 390                 395                 400 
Gln Val Asn Phe Tyr Asp Ser Thr Pro Ala Gly Trp Ala Ile Gly Thr 
                405                 410                 415     
Phe Gly Asn Trp Pro Thr Thr Pro Trp Leu Gln Asn Thr Trp Gln Phe 
            420                 425                 430         
Val Arg Pro Phe Leu Ala Asp Leu Ala Lys Arg Tyr Pro Thr Glu Gly 
        435                 440                 445             
Gly Ile Tyr Leu Ser Glu Phe Gly Phe Ser Glu Pro Phe Glu Asn Asp 
    450                 455                 460                 
Lys Thr Phe Ile Tyr Gln Ile Thr Gln Asp Ser Gly Arg Thr Ala Tyr 
465                 470                 475                 480 
Phe Asn Ser Tyr Leu Gly Glu Val Leu Lys Gly Ile Val Glu Asp Gly 
                485                 490                 495     
Ile Pro Ile Lys Gly Val Phe Gly Trp Ser Met Val Asp Asn Phe Glu 
            500                 505                 510         
Trp Asn Ser Gly Leu Ser Thr Arg Phe Gly Val Gln Tyr Val Asp Tyr 
        515                 520                 525             
Asn Ser Pro Thr Arg Gln Arg Thr Phe Lys Arg Ser Ala Leu Glu Met 
    530                 535                 540                 
Ser Glu Phe Trp Asn Ala His Arg Cys Ser Ala 
545                 550                 555 


