                         SEQUENCE LISTING

<110>  CRISPR THERAPEUTICS AG
       BAYER HEALTHCARE, LLC
       CAFFERTY, Brian Joseph, et. al.
 
<120>  METHODS OF SYNTHESIZING RNA MOLECULES

<130>  CBTN-034PC

<140>  PCT/US2020/062342
<141>  2020-11-25

<150>  US 62/941,174
<151>  2019-11-27

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: residues 47-57 of HIV-1 TAT

<400>  1

Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 
1               5                   10      


<210>  2
<211>  33
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1

<400>  2
gagaacgcac cacuuuacga guuuuagagc uag                                    33


<210>  3
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate

<400>  3
aaauagcaag uuaaaauaag gcuaguccgu uaucaacuu                              39


<210>  4
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 3


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate

<400>  4
gaaaaagugg caccgagucg gugcuuuu                                          28


<210>  5
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Splint 1->2

<400>  5
ccttatttta acttgctatt tctagctcta aaactc                                 36


<210>  6
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Splint 2->3

<400>  6
aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcc                    48


<210>  7
<211>  33
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (2)..(4)
<223>  2'-O-methyl with phosphorothioate

<220>
<221>  modified_base
<222>  (29)..(32)
<223>  2'-O-methyl

<400>  7
gagaacgcac cacuuuacga guuuuagagc uag                                    33


<210>  8
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2m1


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate

<220>
<221>  modified_base
<222>  (4)..(7)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (35)..(39)
<223>  2'-O-methyl

<400>  8
aaauagcaag uuaaaauaag gcuaguccgu uaucaacuu                              39


<210>  9
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2m2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate

<220>
<221>  modified_base
<222>  (4)..(7)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (35)..(37)
<223>  2'-O-methyl

<400>  9
aaauagcaag uuaaaauaag gcuaguccgu uaucaacuu                              39


<210>  10
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 3


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate

<220>
<221>  modified_base
<222>  (5)..(25)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (26)..(28)
<223>  2'-O-methyl with phosphorothioate

<400>  10
gaaaaagugg caccgagucg gugcuuuu                                          28


<210>  11
<211>  33
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-methyl with phosphorothioate

<220>
<221>  modified_base
<222>  (29)..(33)
<223>  2'-O-methyl

<400>  11
uggacagcgu ccauacuggu guuuuagagc uag                                    33


<210>  12
<211>  39
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate, 2'-O-methyl

<220>
<221>  modified_base
<222>  (2)..(7)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (36)..(39)
<223>  2'-O-methyl

<400>  12
aaauagcaag uuaaaauaag gcuaguccgu uaucaacuu                              39


<210>  13
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 3


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate, 2'-O-methyl

<220>
<221>  modified_base
<222>  (2)..(24)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (25)..(27)
<223>  2'-O-methyl with phosphorothioate

<220>
<221>  modified_base
<222>  (28)..(28)
<223>  2'-O-methyl

<400>  13
gaaaaagugg caccgagucg gugcuuuu                                          28


<210>  14
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Splint 1 alt

<400>  14
ccttatttta acttgctatt tctagctcta aaacac                                 36


<210>  15
<211>  33
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1

<400>  15
uggacagcgu ccauacuggu guuuuagagc uag                                    33


<210>  16
<211>  110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SpyCas9 sgRNA


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  n, if present, is a, c, g, or u

<220>
<221>  misc_feature
<222>  (16)..(30)
<223>  n may or may not be present

<400>  16
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                 110


<210>  17
<211>  80
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: SpyCas9 sgRNA backbone

<400>  17
guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu       60

ggcaccgagu cggugcuuuu                                                   80


<210>  18
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G6PC Spacer Sequence unmodified

<400>  18
uggacagcgu ccauacuggu                                                   20


<210>  19
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Unmodified G6PC sgRNA

<400>  19
uggacagcgu ccauacuggu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  20
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Modified G6PC sgRNA


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-methyl with phosphorothioate

<220>
<221>  modified_base
<222>  (29)..(40)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (69)..(96)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (97)..(99)
<223>  2'-O-methyl with phosphorothioate

<220>
<221>  modified_base
<222>  (100)..(100)
<223>  2'-O-methyl

<400>  20
uggacagcgu ccauacuggu guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  21
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: G6PC Spacer sequence modified


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-methyl with phosphorothioate

<400>  21
uggacagcgu ccauacuggu                                                   20


<210>  22
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 1 complement to RNA 1

<400>  22
ctagctctaa aactc                                                        15


<210>  23
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1 complement to DNA splint 1

<400>  23
guguuuuaga gcuag                                                        15


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 1 complement to RNA 2

<400>  24
ccttatttta acttgctatt t                                                 21


<210>  25
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2 complement to DNA splint 1

<400>  25
aaauagcaag uuaaaauaag g                                                 21


<210>  26
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 2 complement to RNA 2

<400>  26
aagttgataa cggactag                                                     18


<210>  27
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2 complement to DNA splint 2

<400>  27
cuaguccguu aucaacuu                                                     18


<210>  28
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 2 complement to RNA 3

<400>  28
aaaagcaccg actcggtgcc actttttc                                          28


<210>  29
<211>  28
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 3 complement to DNA splint 2

<400>  29
gaaaaagugg caccgagucg gugcuuuu                                          28


<210>  30
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 1 complement to RNA 1

<400>  30
tctagctcta aaac                                                         14


<210>  31
<211>  14
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1 complement to DNA splint 1

<400>  31
guuuuagagc uaga                                                         14


<210>  32
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 1 complement to RNA 2

<400>  32
ttattttaac ttgctatt                                                     18


<210>  33
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2 complement to DNA splint 1

<400>  33
aauagcaagu uaaaauaa                                                     18


<210>  34
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 2 complement to RNA 2

<400>  34
tgataacgga ctagcc                                                       16


<210>  35
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2 complement to DNA splint 2

<400>  35
ggcuaguccg uuauca                                                       16


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 2 complement to RNA 3

<400>  36
tcggtgccac tttttcaagt                                                   20


<210>  37
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 3 complement to DNA splint 2

<400>  37
acuugaaaaa guggcaccga                                                   20


<210>  38
<211>  34
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Unmodified RNA 1

<400>  38
uggacagcgu ccauacuggu guuuuagagc uaga                                   34


<210>  39
<211>  34
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Modified RNA 1


<220>
<221>  modified_base
<222>  (1)..(3)
<223>  2'-O-methyl with phosphorothioate

<220>
<221>  modified_base
<222>  (29)..(34)
<223>  2'-O-methyl

<400>  39
uggacagcgu ccauacuggu guuuuagagc uaga                                   34


<210>  40
<211>  34
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Unmodified RNA 2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate

<400>  40
aauagcaagu uaaaauaagg cuaguccguu auca                                   34


<210>  41
<211>  34
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Modified RNA 2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate, 2'-O-methyl

<220>
<221>  modified_base
<222>  (2)..(6)
<223>  2'-O-methyl

<400>  41
aauagcaagu uaaaauaagg cuaguccguu auca                                   34


<210>  42
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Unmodified RNA 3


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate

<400>  42
acuugaaaaa guggcaccga gucggugcuu uu                                     32


<210>  43
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Modified RNA 3


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  phosphate, 2'-O-methyl

<220>
<221>  modified_base
<222>  (2)..(28)
<223>  2'-O-methyl

<220>
<221>  modified_base
<222>  (29)..(31)
<223>  2'-O-methyl with phosphorothioate

<220>
<221>  modified_base
<222>  (32)..(32)
<223>  2'-O-methyl

<400>  43
acuugaaaaa guggcaccga gucggugcuu uu                                     32


<210>  44
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA Splint 1

<400>  44
ttattttaac ttgctatttc tagctctaaa ac                                     32


<210>  45
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA Splint 2

<400>  45
tcggtgccac tttttcaagt tgataacgga ctagcc                                 36


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 1 complement to RNA 1

<400>  46
tctagctcta aaacaccagt atg                                               23


<210>  47
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1 complement to DNA splint 1

<400>  47
cauacuggug uuuuagagcu aga                                               23


<210>  48
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 1 complement to RNA 2

<400>  48
tattttaact tgctatt                                                      17


<210>  49
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2 complement to DNA splint 1

<400>  49
aauagcaagu uaaaaua                                                      17


<210>  50
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 2 complement to RNA 2

<400>  50
tgataacgga ctagcct                                                      17


<210>  51
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2 complement to DNA splint 2

<400>  51
aggcuagucc guuauca                                                      17


<210>  52
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA Splint 1

<400>  52
tattttaact tgctatttct agctctaaaa caccagtatg                             40


<210>  53
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA Splint 2

<400>  53
gactcggtgc cactttttca agttgataac ggactagcct                             40


<210>  54
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA splint 2 complement to RNA 3

<400>  54
gactcggtgc cactttttca agt                                               23


<210>  55
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 3 complement to DNA splint 2

<400>  55
acuugaaaaa guggcaccga guc                                               23


<210>  56
<211>  43
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  n, if present, is a, c, g, or u

<220>
<221>  misc_feature
<222>  (16)..(30)
<223>  n may or may not be present

<400>  56
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn guuuuagagc uag                         43


<210>  57
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 1


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  n, if present, is a, c, g, or u

<220>
<221>  misc_feature
<222>  (16)..(30)
<223>  n may or may not be present

<400>  57
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn guuuuagagc uaga                        44


<210>  58
<211>  35
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  Phosphate

<400>  58
aaauagcaag uuaaaauaag gcuaguccgu uauca                                  35


<210>  59
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: RNA 2

<400>  59
aauagcaagu uaaaauaagg cuaguccguu aucaacuu                               38


<210>  60
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Splint 1

<400>  60
ccttatttta acttgctatt tctagctcta aaac                                   34


<210>  61
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Splint 1

<400>  61
tattttaact tgctatttct agctctaaaa c                                      31


