                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Riley, James L.
       Ellis, Gavin 
       Xu, Jun 
       Melenhorst, Jan J.
 
<120>  Anti-CD3 scFv and Cytokine Producing Artificial Antigen 
       Presenting Cells

<130>  046483-7268WO1(02461)

<150>  U.S. Provisional Patent Application No. 62/941,062
<151>  2019-11-27

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q HCDR1

<400>  1

Gly Phe Thr Phe Asn Ser Tyr Ala 
1               5               


<210>  2
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q HCDR2

<400>  2

Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr 
1               5                   10  


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q HCDR3

<400>  3

Val Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Trp Ala Tyr 
1               5                   10                  15      


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q LCDR1

<400>  4

Thr Gly Ala Val Thr Ser Gly Asn Tyr 
1               5                   


<210>  5
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q LCDR3

<400>  5

Val Leu Trp Tyr Ser Asn Arg Trp Val 
1               5                   


<210>  6
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q VH

<400>  6

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Ser Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Trp 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  7
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F112Q VL

<400>  7

Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly 
            20                  25                  30          


Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val 
65                  70                  75                  80  


Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn 
                85                  90                  95      


Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  8
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q scFv

<400>  8

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Ser Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Trp 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Thr Val Val 
    130                 135                 140                 


Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu 
145                 150                 155                 160 


Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly Asn Tyr Pro Asn 
                165                 170                 175     


Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly Leu Ile Gly Gly 
            180                 185                 190         


Thr Lys Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu 
        195                 200                 205             


Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val Gln Pro Glu Asp 
    210                 215                 220                 


Glu Ala Glu Tyr Tyr Cys Val Leu Trp Tyr Ser Asn Arg Trp Val Phe 
225                 230                 235                 240 


Gly Gly Gly Thr Lys Leu Thr Val Leu 
                245                 


<210>  9
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q CAR

<400>  9

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Thr Phe Asn Ser Tyr Ala Met Asn Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn 
65                  70                  75                  80  


Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser 
                85                  90                  95      


Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Asn Leu Lys 
            100                 105                 110         


Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His Gly Asn Phe Gly 
        115                 120                 125             


Asn Ser Tyr Val Ser Trp Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val 
    130                 135                 140                 


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser 
                165                 170                 175     


Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val 
            180                 185                 190         


Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala 
        195                 200                 205             


Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro 
    210                 215                 220                 


Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu 
225                 230                 235                 240 


Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp 
                245                 250                 255     


Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            260                 265                 270         


Ser Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
        275                 280                 285             


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
    290                 295                 300                 


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
305                 310                 315                 320 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
                325                 330                 335     


Leu Val Ile Thr Leu Tyr Cys Arg Val Lys Phe Ser Arg Ser Ala Asp 
            340                 345                 350         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        355                 360                 365             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    370                 375                 380                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                405                 410                 415     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            420                 425                 430         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        435                 440                 445             


Met Gln Ala Leu Pro Pro Arg 
    450                 455 


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 HCDR1

<400>  10

Gly Tyr Thr Phe Thr Arg Tyr Thr 
1               5               


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 HCDR2

<400>  11

Ile Asn Pro Ser Arg Gly Tyr Thr 
1               5               


<210>  12
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 HCDR3

<400>  12

Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr 
1               5                   10          


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 LCDR1

<400>  13

Ser Ser Val Ser Tyr 
1               5   


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 LCDR3

<400>  14

Gln Gln Trp Ser Ser Asn Pro Phe Thr 
1               5                   


<210>  15
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 VH

<400>  15

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  16
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 VL

<400>  16

Ser Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ala His Phe Arg Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Gly Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Ile Asn 
            100                 105     


<210>  17
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 scFv

<400>  17

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Ile Val Leu Thr Gln Ser Pro Ala Ile 
    130                 135                 140                 


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ser 
145                 150                 155                 160 


Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser 
                165                 170                 175     


Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro 
            180                 185                 190         


Ala His Phe Arg Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
        195                 200                 205             


Ser Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 
    210                 215                 220                 


Ser Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Asn 
225                 230                 235                 240 


Arg Ala Ala Ala 
                


<210>  18
<211>  339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 CAR

<400>  18

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu 
            20                  25                  30          


Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ile Val Leu Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
                165                 170                 175     


Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ala His Phe Arg Gly Ser Gly Ser Gly Thr Ser 
    210                 215                 220                 


Tyr Ser Leu Thr Ile Ser Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Asn Arg Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys 
            


<210>  19
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q CAR Intracellular Domain

<400>  19

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  20
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 CAR Intracellular Domain

<400>  20

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys 
65                  70                  


<210>  21
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 Hinge Domain

<400>  21

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  22
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 TM Domain

<400>  22

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  23
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F12Q chimeric receptor

<400>  23

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Thr Phe Asn Ser Tyr Ala Met Asn Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Tyr Asn Asn 
65                  70                  75                  80  


Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser 
                85                  90                  95      


Arg Asp Asp Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Asn Leu Lys 
            100                 105                 110         


Thr Glu Asp Thr Ala Val Tyr Tyr Cys Val Arg His Gly Asn Phe Gly 
        115                 120                 125             


Asn Ser Tyr Val Ser Trp Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val 
    130                 135                 140                 


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser 
                165                 170                 175     


Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val 
            180                 185                 190         


Thr Ser Gly Asn Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala 
        195                 200                 205             


Pro Arg Gly Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro 
    210                 215                 220                 


Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu 
225                 230                 235                 240 


Ser Gly Val Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Val Leu Trp 
                245                 250                 255     


Tyr Ser Asn Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            260                 265                 270         


Ser Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
        275                 280                 285             


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
    290                 295                 300                 


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
305                 310                 315                 320 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
                325                 330                 335     


Leu Val Ile Thr Leu Tyr Cys Arg Val Lys Phe Ser Arg Ser Ala Asp 
            340                 345                 350         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        355                 360                 365             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    370                 375                 380                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                405                 410                 415     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            420                 425                 430         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        435                 440                 445             


Met Gln Ala Leu Pro Pro Arg 
    450                 455 


<210>  24
<211>  339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OKT3 chimeric receptor

<400>  24

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu 
            20                  25                  30          


Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ile Val Leu Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
                165                 170                 175     


Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ala His Phe Arg Gly Ser Gly Ser Gly Thr Ser 
    210                 215                 220                 


Tyr Ser Leu Thr Ile Ser Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Asn Arg Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys 
            


<210>  25
<211>  1368
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F12Q chimeric receptor

<400>  25
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgggatccg aagtacaact tgtagagtct ggagggggac tcgtccagcc cggtggctcc      120

ctgaaacttt catgcgcagc aagcgggttc acgtttaact cttatgcaat gaattgggtc      180

cgacaagctc caggcaaagg acttgaatgg gtcgccagga ttcggagcaa atacaacaac      240

tacgcgacat attatgctga tagtgtaaaa ggacggttca cgatctcaag agacgattct      300

aagaatacag cgtatcttca aatgaacaac ttgaaaacag aagacaccgc cgtttattat      360

tgcgtcagac atggcaactt tggaaacagt tacgtttctt ggtgggccta ctggggtcaa      420

gggacgcttg tcaccgtcag ctcaggaggc gggggatctg gcggcggcgg ttcaggtggt      480

ggtggatctc agacagtggt cacacaagag ccatcactta ccgtatcccc tggggggact      540

gtaaccctta cctgcgggtc ctctacgggg gctgttacta gcggcaacta tccaaactgg      600

gtgcaacaaa agcctggtca agctccccgc gggctgatcg gcggtactaa gttccttgcg      660

ccgggcactc ctgcaagatt ttcaggttct cttcttgggg gaaaagctgc attgactctc      720

agtggtgtcc agccggagga cgaagccgag tattactgtg tcctctggta ttccaatagg      780

tgggtgttcg ggggtggtac taaactcact gtgctttccg gaaccacgac gccagcgccg      840

cgaccaccaa caccggcgcc caccatcgcg tcgcagcccc tgtccctgcg cccagaggcg      900

tgccggccag cggcgggggg cgcagtgcac acgagggggc tggacttcgc ctgtgatatc      960

tacatctggg cgcccttggc cgggacttgt ggggtccttc tcctgtcact ggttatcacc     1020

ctttactgca gagtgaagtt cagcaggagc gcagacgccc ccgcgtacca gcagggccag     1080

aaccagctct ataacgagct caatctagga cgaagagagg agtacgatgt tttggacaag     1140

agacgtggcc gggaccctga gatgggggga aagccgagaa ggaagaaccc tcaggaaggc     1200

ctgtacaatg aactgcagaa agataagatg gcggaggcct acagtgagat tgggatgaaa     1260

ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc agggtctcag tacagccacc     1320

aaggacacct acgacgccct tcacatgcag gccctgcccc ctcgctaa                  1368


<210>  26
<211>  1023
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OKT3 chimeric receptor

<400>  26
atggcactgc ccgtgaccgc cctcctcctg cccctcgcgc tactcctgca cgccgccaga       60

ccccaggtgc agctgcagca gagtggcgct gagctggccc gccccggcgc ctccgtgaag      120

atgtcctgca aggctagtgg gtataccttc accaggtata ctatgcactg ggtgaagcag      180

cgtccggggc aggggctcga gtggatcggc tacatcaatc cctcccgcgg ctacaccaat      240

tacaaccaga agttcaagga taaggccacg ctgaccacag acaagagtag ctccacggcc      300

tacatgcagt tatcaagtct gacctctgag gactccgctg tgtactattg tgcgaggtac      360

tacgacgacc actactgtct ggactactgg ggccaaggca caaccctgac tgtaagttcc      420

tccggcggcg gggggtccgg cggcggcggc tccggcgggg ggggtagtat cgtgctgaca      480

cagagtcccg caatcatgtc cgcaagcccc ggagagaagg tgaccatgac gtgtagtgct      540

tccagctccg tgtcctatat gaactggtac cagcagaaat ccgggacttc ccccaagaga      600

tggatctacg acaccagtaa gctggccagt ggcgtgcctg cacacttccg cggcagtggc      660

tccggcacta gttacagtct caccatctcc gggatggaag ctgaggacgc cgctacctac      720

tactgccagc agtggagctc gaacccattc accttcggtt cggggaccaa gctcgagatc      780

aacagggcgg ccgccaccac gacgccagcg ccgcgaccac caacaccggc gcccaccatc      840

gcgtcgcagc ccctgtccct gcgcccagag gcgtgccggc cagcggcggg gggcgcagtg      900

cacacgaggg ggctggactt cgcctgtgat atctacatct gggcgccctt ggccgggact      960

tgtggggtcc ttctcctgtc actggttatc accctttact gcaaacgggg cagaaagtaa     1020

taa                                                                   1023


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  Repeat N times, where N = an integer of at least 1.

<400>  27

Gly Ser Gly Gly Ser 
1               5   


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  Repeat N times, where N = an integer of at least 1.

<400>  28

Gly Gly Gly Ser 
1               


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  Repeat N times, where N = an integer of at least 1.

<400>  29

Gly Gly Gly Gly Ser 
1               5   


<210>  30
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  30

Gly Gly Ser Gly 
1               


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  31

Gly Gly Ser Gly Gly 
1               5   


<210>  32
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  32

Gly Ser Gly Ser Gly 
1               5   


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  33

Gly Ser Gly Gly Gly 
1               5   


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  34

Gly Gly Gly Ser Gly 
1               5   


<210>  35
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  35

Gly Ser Ser Ser Gly 
1               5   


<210>  36
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  36

Gly Gly Gly Gly Ser 
1               5   


<210>  37
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  37

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  38
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GS Linker Nuceotide

<400>  38
ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatct                       45


<210>  39
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  39

Asp Lys Thr His Thr 
1               5   


<210>  40
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  40

Cys Pro Pro Cys 
1               


<210>  41
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  41

Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
1               5                   10                  15  


<210>  42
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  42

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
1               5                   10          


<210>  43
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  43

Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
1               5                   10  


<210>  44
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  44

Lys Cys Cys Val Asp Cys Pro 
1               5           


<210>  45
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  45

Lys Tyr Gly Pro Pro Cys Pro 
1               5           


<210>  46
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  46

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  47
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  47

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  48
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  48

Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      


Pro 
    


<210>  49
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  49

Ser Pro Asn Met Val Pro His Ala His His Ala Gln 
1               5                   10          


<210>  50
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge

<400>  50

Glu Pro Lys Ser Cys Asp Lys Thr Tyr Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


