                         SEQUENCE LISTING

<110>  DuPont Nutrition Biosciences ApS
 
<120>  ENGINEERED ENZYMES

<130>  NB41752-WO-PCT

<150>  US 62/937904
<151>  2019-11-20

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  413
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1

<400>  1

Asn Asp Thr Pro Ala Ser Gly Tyr Gln Val Glu Lys Val Val Ile Leu 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met Arg 
            20                  25                  30          


Asp Val Thr Pro Asn Thr Trp Pro Glu Trp Pro Val Lys Leu Gly Tyr 
        35                  40                  45              


Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe Tyr 
    50                  55                  60                  


Arg Gln Lys Phe Gln Gln Gln Gly Ile Leu Ser Gln Gly Ser Cys Pro 
65                  70                  75                  80  


Thr Pro Asn Ser Ile Tyr Val Trp Thr Asp Val Ala Gln Arg Thr Leu 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Gly Leu 
            100                 105                 110         


Thr Ile His His Gln Gln Asn Leu Glu Lys Ala Asp Pro Leu Phe His 
        115                 120                 125             


Pro Val Lys Ala Gly Ile Cys Ser Met Asp Lys Thr Gln Val Gln Gln 
    130                 135                 140                 


Ala Val Glu Lys Glu Ala Gln Thr Pro Ile Asp Asn Leu Asn Gln His 
145                 150                 155                 160 


Tyr Ile Pro Ser Leu Ala Leu Met Asn Thr Thr Leu Asn Phe Ser Lys 
                165                 170                 175     


Ser Pro Trp Cys Gln Lys His Ser Ala Asp Lys Ser Cys Asp Leu Gly 
            180                 185                 190         


Leu Ser Met Pro Ser Lys Leu Ser Ile Lys Asp Asn Gly Asn Glu Val 
        195                 200                 205             


Ser Leu Asp Gly Ala Ile Gly Leu Ser Ser Thr Leu Ala Glu Ile Phe 
    210                 215                 220                 


Leu Leu Glu Tyr Ala Gln Gly Met Pro Gln Ala Ala Trp Gly Asn Ile 
225                 230                 235                 240 


His Ser Glu Gln Glu Trp Ala Leu Leu Leu Lys Leu His Asn Val Tyr 
                245                 250                 255     


Phe Asp Leu Met Glu Arg Thr Pro Tyr Ile Ala Arg His Lys Gly Thr 
            260                 265                 270         


Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu Asn Pro Asn Ala Thr Glu 
        275                 280                 285             


Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile Ala 
    290                 295                 300                 


Gly His Asp Thr Asn Ile Ala Asn Ile Ala Gly Met Leu Asn Met Arg 
305                 310                 315                 320 


Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala Leu 
                325                 330                 335     


Val Phe Glu Arg Leu Ala Asp Lys Ser Gly Lys Gln Tyr Val Ser Val 
            340                 345                 350         


Ser Met Val Tyr Gln Thr Leu Glu Gln Leu Arg Ser Gln Thr Pro Leu 
        355                 360                 365             


Ser Leu Asn Gln Pro Ala Gly Ser Val Gln Leu Lys Ile Pro Gly Cys 
    370                 375                 380                 


Asn Asp Gln Thr Ala Glu Gly Tyr Cys Pro Leu Ser Thr Phe Thr Arg 
385                 390                 395                 400 


Val Val Ser Gln Ser Val Glu Pro Gly Cys Gln Leu Gln 
                405                 410             


<210>  2
<211>  413
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1

<400>  2

Asn Asp Thr Pro Ala Ser Gly Tyr Gln Val Glu Lys Val Val Ile Leu 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met Arg 
            20                  25                  30          


Asp Val Thr Pro Asn Thr Trp Pro Glu Trp Pro Val Lys Leu Gly Tyr 
        35                  40                  45              


Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe Tyr 
    50                  55                  60                  


Arg Glu Lys Phe Gln Gln Gln Gly Ile Leu Ser Gln Gly Ser Cys Pro 
65                  70                  75                  80  


Thr Pro Asn Ser Ile Tyr Val Trp Ala Asp Val Asp Gln Arg Thr Leu 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Gly Leu 
            100                 105                 110         


Thr Ile His His Gln Gln Asn Leu Glu Lys Ala Asp Pro Leu Phe His 
        115                 120                 125             


Pro Val Lys Ala Gly Thr Cys Ser Met Asp Lys Thr Arg Leu Gln Gln 
    130                 135                 140                 


Ala Val Glu Lys Glu Ala Gln Thr Pro Ile Glu Asn Leu Asn Gln His 
145                 150                 155                 160 


Tyr Ile Pro Ser Leu Ala Leu Met Asn Thr Thr Leu Asn Phe Ser Thr 
                165                 170                 175     


Ser Ala Trp Cys Gln Lys His Ser Ala Asp Lys Ser Cys Asp Leu Ala 
            180                 185                 190         


Gln Ser Met Pro Ser Lys Leu Ser Ile Lys Asp Asn Gly Asn Lys Val 
        195                 200                 205             


Ala Leu Asp Gly Ala Val Gly Leu Ser Ser Thr Leu Ala Glu Ile Phe 
    210                 215                 220                 


Leu Leu Glu Tyr Ala Gln Gly Met Pro Gln Ala Ala Trp Gly Lys Ile 
225                 230                 235                 240 


His Ser Glu Gln Asp Trp Ala Glu Leu Leu Lys Leu His Asn Ala Gln 
                245                 250                 255     


Phe Asp Leu Met Ala Arg Thr Pro Tyr Ile Ala Arg His Asn Gly Thr 
            260                 265                 270         


Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu Asp Pro Asn Ala Thr Ala 
        275                 280                 285             


Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile Ala 
    290                 295                 300                 


Gly His Asp Thr Asn Ile Ala Asn Ile Ser Gly Met Leu Asn Met Arg 
305                 310                 315                 320 


Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala Leu 
                325                 330                 335     


Ile Phe Glu Arg Leu Ala Asp Lys Ala Gly Lys Gln Tyr Val Ser Val 
            340                 345                 350         


Ser Met Val Tyr Gln Thr Leu Glu Gln Leu Arg Ala Gln Thr Pro Leu 
        355                 360                 365             


Ser Leu Lys Glu Pro Ala Gly Ser Val Gln Leu Lys Ile Pro Gly Cys 
    370                 375                 380                 


Asn Asp Gln Thr Ala Glu Gly Tyr Cys Pro Leu Pro Thr Phe Lys Arg 
385                 390                 395                 400 


Val Val Ser Gln Ser Glu Glu Pro Gly Cys Gln Leu Gln 
                405                 410             


<210>  3
<211>  413
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1

<400>  3

Ser Asp Thr Ala Pro Ala Gly Phe Gln Leu Glu Lys Val Val Ile Leu 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met Arg 
            20                  25                  30          


Asp Val Thr Pro His Gln Trp Pro Glu Trp Pro Val Lys Leu Gly Tyr 
        35                  40                  45              


Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe Tyr 
    50                  55                  60                  


Arg Glu Arg Phe Gln Gln Gln Gly Leu Leu Pro Lys Asp Asn Cys Pro 
65                  70                  75                  80  


Thr Pro Asp Ala Val Tyr Val Trp Ala Asp Val Asp Gln Arg Thr Arg 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Asp Leu 
            100                 105                 110         


Ala Ile His His Gln Gln Asn Thr Gln Gln Ala Asp Pro Leu Phe His 
        115                 120                 125             


Pro Val Lys Ala Gly Ile Cys Ser Met Asp Lys Ser Gln Val His Ala 
    130                 135                 140                 


Ala Val Glu Lys Gln Ala Gly Thr Pro Ile Glu Thr Leu Asn Gln Arg 
145                 150                 155                 160 


Tyr Gln Ala Ser Leu Ala Leu Met Ser Ser Val Leu Asp Phe Pro Lys 
                165                 170                 175     


Ser Pro Tyr Cys Gln Gln His Asn Ile Gly Lys Leu Cys Asp Phe Ser 
            180                 185                 190         


Gln Ala Met Pro Ser Arg Leu Ala Ile Asn Asp Asp Gly Asn Lys Val 
        195                 200                 205             


Ala Leu Glu Gly Ala Val Gly Leu Ala Ser Thr Leu Ala Glu Ile Phe 
    210                 215                 220                 


Leu Leu Glu His Ala Gln Gly Met Pro Lys Val Ala Trp Gly Asn Ile 
225                 230                 235                 240 


His Thr Glu Gln Gln Trp Asn Ser Leu Leu Lys Leu His Asn Ala Gln 
                245                 250                 255     


Phe Asp Leu Met Ser Arg Thr Pro Tyr Ile Ala Lys His Asn Gly Thr 
            260                 265                 270         


Pro Leu Leu Gln Thr Ile Ala His Ala Leu Gly Ser Asn Ile Thr Ser 
        275                 280                 285             


Arg Pro Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile Ala 
    290                 295                 300                 


Gly His Asp Thr Asn Ile Ala Asn Ile Ser Gly Met Leu Gly Met Thr 
305                 310                 315                 320 


Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Ala Leu 
                325                 330                 335     


Val Phe Glu Arg Trp Val Asp Asn Ala Gly Lys Pro Tyr Val Ser Val 
            340                 345                 350         


Asn Met Val Tyr Gln Thr Leu Ala Gln Leu His Asp Gln Ala Pro Leu 
        355                 360                 365             


Thr Leu Gln His Pro Ala Gly Ser Val Arg Leu Asn Ile Pro Gly Cys 
    370                 375                 380                 


Ser Asp Gln Thr Pro Asp Gly Tyr Cys Pro Leu Ser Thr Phe Ser Arg 
385                 390                 395                 400 


Leu Val Ser His Ser Val Glu Pro Ala Cys Gln Leu Pro 
                405                 410             


<210>  4
<211>  413
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1

<400>  4

His Ala Glu Glu Gln Asn Gly Met Lys Leu Glu Arg Val Val Ile Val 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Phe Thr Pro Ile Met Lys 
            20                  25                  30          


Asp Val Thr Pro Asp Gln Trp Pro Gln Trp Asp Val Pro Leu Gly Trp 
        35                  40                  45              


Leu Thr Pro Arg Gly Gly Glu Leu Val Ser Glu Leu Gly Gln Tyr Gln 
    50                  55                  60                  


Arg Leu Trp Phe Thr Ser Lys Gly Leu Leu Asn Asn Gln Thr Cys Pro 
65                  70                  75                  80  


Ser Pro Gly Gln Val Ala Val Ile Ala Asp Thr Asp Gln Arg Thr Arg 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Lys Cys Gln Ile 
            100                 105                 110         


Gln Val His Tyr Gln Lys Asp Glu Glu Lys Asn Asp Pro Leu Phe Asn 
        115                 120                 125             


Pro Val Lys Met Gly Lys Cys Ser Phe Asn Thr Leu Lys Val Lys Asn 
    130                 135                 140                 


Ala Ile Leu Glu Arg Ala Gly Gly Asn Ile Glu Leu Tyr Thr Gln Arg 
145                 150                 155                 160 


Tyr Gln Ser Ser Phe Arg Thr Leu Glu Asn Val Leu Asn Phe Ser Gln 
                165                 170                 175     


Ser Glu Thr Cys Lys Thr Thr Glu Lys Ser Thr Lys Cys Thr Leu Pro 
            180                 185                 190         


Glu Ala Leu Pro Ser Glu Phe Lys Val Thr Pro Asp Asn Val Ser Leu 
        195                 200                 205             


Pro Gly Ala Trp Ser Leu Ser Ser Thr Leu Thr Glu Ile Phe Leu Leu 
    210                 215                 220                 


Gln Glu Ala Gln Gly Met Pro Gln Val Ala Trp Gly Arg Ile Thr Gly 
225                 230                 235                 240 


Glu Lys Glu Trp Arg Asp Leu Leu Ser Leu His Asn Ala Gln Phe Asp 
                245                 250                 255     


Leu Leu Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro Leu 
            260                 265                 270         


Leu Asp Met Ile Asp Thr Ala Leu Leu Thr Asn Gly Thr Thr Glu Asn 
        275                 280                 285             


Arg Tyr Gly Ile Lys Leu Pro Val Ser Leu Leu Phe Ile Ala Gly His 
    290                 295                 300                 


Asp Thr Asn Leu Ala Asn Leu Ser Gly Ala Leu Asp Leu Lys Trp Ser 
305                 310                 315                 320 


Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Glu Leu Val Phe 
                325                 330                 335     


Glu Lys Trp Lys Arg Thr Ser Asp Asn Thr Asp Trp Val Gln Val Ser 
            340                 345                 350         


Phe Val Tyr Gln Thr Leu Arg Asp Met Arg Asp Ile Gln Pro Leu Ser 
        355                 360                 365             


Leu Glu Lys Pro Ala Gly Lys Val Asp Leu Lys Leu Ile Ala Cys Glu 
    370                 375                 380                 


Glu Lys Asn Ser Gln Gly Met Cys Ser Leu Lys Ser Phe Ser Arg Leu 
385                 390                 395                 400 


Ile Lys Glu Ile Arg Val Pro Glu Cys Ala Val Thr Glu 
                405                 410             


<210>  5
<211>  414
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1

<400>  5

Ser Asp Thr Ala Pro Ala Gly Phe Gln Leu Glu Lys Val Val Ile Leu 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Met Thr Gln Thr Met Arg 
            20                  25                  30          


Asp Val Thr Pro His Gln Trp Pro Glu Trp Pro Val Lys Leu Gly Tyr 
        35                  40                  45              


Ile Thr Pro Arg Gly Glu His Leu Ile Ser Leu Met Gly Gly Phe Tyr 
    50                  55                  60                  


Arg Glu Arg Phe Gln Gln Gln Gly Leu Leu Pro Lys Asp Asn Cys Pro 
65                  70                  75                  80  


Thr Pro Asp Ala Val Tyr Val Trp Thr Asp Val Asn Gln Arg Thr Arg 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Leu Ala Gly Leu Ala Pro Gln Cys Asp Leu 
            100                 105                 110         


Ala Ile His His Gln Gln Asn Ile Thr Gln Val Asp Pro Leu Phe His 
        115                 120                 125             


Pro Val Lys Ala Gly Ile Cys Ser Met Asn Lys Ser Gln Thr Tyr Glu 
    130                 135                 140                 


Ala Val Glu Lys Gln Ala Gly Gly Pro Ile Glu Thr Leu Asn Gln Arg 
145                 150                 155                 160 


Tyr Gln Ala Glu Leu Ala Leu Met Ser Ser Val Leu Asp Phe Pro Lys 
                165                 170                 175     


Ser Pro Tyr Cys Gln Gln His Asn Ile Gly Lys Leu Cys Asp Phe Ser 
            180                 185                 190         


Gln Ala Met Pro Ser Arg Leu Asn Ile Ser Asp Asp Gly Asn Glu Val 
        195                 200                 205             


Gln Leu Glu Gly Ala Val Gly Leu Gly Ser Thr Leu Ala Glu Ile Phe 
    210                 215                 220                 


Leu Leu Glu Tyr Ala Gln Gly Met Pro Val Val Ala Trp Gly Asn Ile 
225                 230                 235                 240 


His Asn Glu Ser Gln Trp Lys Ser Leu Leu Asn Leu His Asn Ala His 
                245                 250                 255     


Phe Asn Leu Met His Arg Thr Pro Tyr Ile Ala Lys His Gln Gly Thr 
            260                 265                 270         


Pro Leu Leu Gln Ala Ile Ser Asn Ala Leu Asn Pro Asn Ala Thr Glu 
        275                 280                 285             


Ser Lys Leu Pro Asp Ile Ser Pro Asp Asn Lys Ile Leu Phe Ile Ala 
    290                 295                 300                 


Gly His Asp Thr Asn Ile Ala Asn Ile Gly Gly Met Leu Gly Met Asn 
305                 310                 315                 320 


Trp Thr Leu Pro Gly Gln Pro Asp Asn Thr Pro Pro Gly Gly Gly Leu 
                325                 330                 335     


Val Phe Glu Leu Trp Gln Asn Pro Asp Asn His Gln Gln Tyr Val Ala 
            340                 345                 350         


Val Lys Met Ile Tyr Gln Thr Met Asp Gln Leu Arg Asn Ser Glu Lys 
        355                 360                 365             


Leu Asp Leu Lys Ser Asn Pro Ala Gly Ile Val Pro Ile Glu Ile Glu 
    370                 375                 380                 


Gly Cys Glu Asn Ile Gly Thr Asp Lys Leu Cys Gln Leu Asp Thr Phe 
385                 390                 395                 400 


Gln Lys Arg Val Ala Gln Val Ile Glu Pro Ala Cys Gln Ile 
                405                 410                 


<210>  6
<211>  412
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1

<400>  6

Phe Ala Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser Val Val Ile Val 
1               5                   10                  15      


Ser Arg His Gly Val Arg Ala Pro Thr Lys Phe Thr Gln Leu Met Gln 
            20                  25                  30          


Asp Val Thr Pro Asp Ala Trp Pro Thr Trp Pro Val Lys Leu Gly Glu 
        35                  40                  45              


Leu Thr Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu Gly His Tyr Trp 
    50                  55                  60                  


Arg Gln Arg Leu Val Ala Asp Gly Leu Leu Pro Lys Cys Gly Glu Pro 
65                  70                  75                  80  


Gln Ser Gly Gln Val Ala Ile Ile Ala Asp Val Asp Glu Arg Thr Arg 
                85                  90                  95      


Lys Thr Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro Asp Cys Ala Ile 
            100                 105                 110         


Thr Val His His Gln Ala Asp Thr Ser Ser Pro Asp Pro Leu Phe Asn 
        115                 120                 125             


Pro Leu Lys Thr Gly Val Cys Gln Leu Asp Val Ala Asn Val Arg Arg 
    130                 135                 140                 


Ala Ile Leu Glu Arg Ala Gly Gly Ser Ile Ala Asp Phe Thr Arg His 
145                 150                 155                 160 


Tyr Gln Thr Ala Phe Arg Glu Leu Glu Arg Val Leu Asn Phe Pro Gln 
                165                 170                 175     


Ser Asn Leu Cys Leu Lys Arg Glu Lys Gln Asp Glu Ser Cys Ser Leu 
            180                 185                 190         


Thr Gln Ala Leu Pro Ser Glu Leu Lys Val Ser Ala Asp Asp Val Ser 
        195                 200                 205             


Leu Thr Gly Ala Trp Ser Leu Ala Ser Met Leu Thr Glu Ile Phe Leu 
    210                 215                 220                 


Leu Gln Gln Ala Gln Gly Met Pro Glu Pro Gly Trp Gly Arg Ile Thr 
225                 230                 235                 240 


Asp Ser His Gln Trp Asn Thr Leu Leu Ser Leu His Asn Ala Val Phe 
                245                 250                 255     


Asp Leu Leu Gln Arg Thr Pro Glu Val Ala Arg Ser Arg Ala Thr Pro 
            260                 265                 270         


Leu Leu Asp Leu Ile Lys Thr Ala Leu Thr Pro His Pro Pro Gln Lys 
        275                 280                 285             


Gln Ala Tyr Gly Val Thr Leu Pro Thr Ser Val Leu Phe Ile Ala Gly 
    290                 295                 300                 


His Asp Thr Asn Leu Ala Asn Leu Gly Gly Ala Leu Glu Leu Asn Trp 
305                 310                 315                 320 


Thr Leu Pro Gly Gln Pro Asp Asn Tyr Pro Pro Gly Gly Glu Leu Val 
                325                 330                 335     


Phe Glu Arg Trp Arg Arg Leu Ser Asp Asn Ser Gln Trp Ile Gln Val 
            340                 345                 350         


Ser Leu Val Phe Gln Thr Leu Gln Gln Met Arg Asp Lys Thr Pro Leu 
        355                 360                 365             


Ser Leu Asn Thr Pro Pro Gly Glu Val Lys Leu Thr Leu Ala Gly Cys 
    370                 375                 380                 


Glu Glu Arg Asn Ala Gln Gly Met Cys Ser Leu Ala Gly Phe Thr Gln 
385                 390                 395                 400 


Ile Val Asn Glu Ala Arg Ile Pro Ala Cys Ser Leu 
                405                 410         


<210>  7
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1

<400>  7

Met Gln Thr Phe Gly Ala Phe Leu Val Ser Phe Leu Ala Ala Ser Gly 
1               5                   10                  15      


Leu Ala Ala Ala 
            20  


