                         SEQUENCE LISTING

<110>  Corbion Biotech, Inc.
 
<120>  SUCROSE INVERTASE VARIANTS

<130>  P6089933CT

<150>  US 62/937,849
<151>  2019-11-20

<150>  US 62/961,986
<151>  2020-01-16

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  532
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Arg Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Asn Ala Gly Pro Trp Ser Arg Phe Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  2
<211>  532
<212>  PRT
<213>  artificial

<220>
<223>  ScSUC2 I17C mutant

<400>  2

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Cys Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Arg Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Asn Ala Gly Pro Trp Ser Arg Phe Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  3
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSUC2 R28V mutant

<400>  3

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Val Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Asn Ala Gly Pro Trp Ser Arg Phe Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  4
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSUC2 N359K mutant

<400>  4

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Arg Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Lys Ala Gly Pro Trp Ser Arg Phe Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  5
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSUC2 F366M mutant

<400>  5

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Arg Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Asn Ala Gly Pro Trp Ser Arg Met Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  6
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSUC2 I17C-R28V mutant

<400>  6

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Cys Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Val Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Asn Ala Gly Pro Trp Ser Arg Phe Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  7
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSUC2 I17C-R28V-N359K mutant

<400>  7

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Cys Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Val Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Lys Ala Gly Pro Trp Ser Arg Phe Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  8
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSUC2 I17C-R28V-F366M mutant

<400>  8

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Cys Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Val Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Asn Ala Gly Pro Trp Ser Arg Met Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  9
<211>  532
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSUC2 I17C-R28V-N359K-F366M mutant

<400>  9

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Cys Ser Ala Ser Met Thr Asn Glu Thr Ser Asp Val Pro Leu Val His 
            20                  25                  30          


Phe Thr Pro Asn Lys Gly Trp Met Asn Asp Pro Asn Gly Leu Trp Tyr 
        35                  40                  45              


Asp Glu Lys Asp Ala Lys Trp His Leu Tyr Phe Gln Tyr Asn Pro Asn 
    50                  55                  60                  


Asp Thr Val Trp Gly Thr Pro Leu Phe Trp Gly His Ala Thr Ser Asp 
65                  70                  75                  80  


Asp Leu Thr Asn Trp Glu Asp Gln Pro Ile Ala Ile Ala Pro Lys Arg 
                85                  90                  95      


Asn Asp Ser Gly Ala Phe Ser Gly Ser Met Val Val Asp Tyr Asn Asn 
            100                 105                 110         


Thr Ser Gly Phe Phe Asn Asp Thr Ile Asp Pro Arg Gln Arg Cys Val 
        115                 120                 125             


Ala Ile Trp Thr Tyr Asn Thr Pro Glu Ser Glu Glu Gln Tyr Ile Ser 
    130                 135                 140                 


Tyr Ser Leu Asp Gly Gly Tyr Thr Phe Thr Glu Tyr Gln Lys Asn Pro 
145                 150                 155                 160 


Val Leu Ala Ala Asn Ser Thr Gln Phe Arg Asp Pro Lys Val Phe Trp 
                165                 170                 175     


Tyr Glu Pro Ser Gln Lys Trp Ile Met Thr Ala Ala Lys Ser Gln Asp 
            180                 185                 190         


Tyr Lys Ile Glu Ile Tyr Ser Ser Asp Asp Leu Lys Ser Trp Lys Leu 
        195                 200                 205             


Glu Ser Ala Phe Ala Asn Glu Gly Phe Leu Gly Tyr Gln Tyr Glu Cys 
    210                 215                 220                 


Pro Gly Leu Ile Glu Val Pro Thr Glu Gln Asp Pro Ser Lys Ser Tyr 
225                 230                 235                 240 


Trp Val Met Phe Ile Ser Ile Asn Pro Gly Ala Pro Ala Gly Gly Ser 
                245                 250                 255     


Phe Asn Gln Tyr Phe Val Gly Ser Phe Asn Gly Thr His Phe Glu Ala 
            260                 265                 270         


Phe Asp Asn Gln Ser Arg Val Val Asp Phe Gly Lys Asp Tyr Tyr Ala 
        275                 280                 285             


Leu Gln Thr Phe Phe Asn Thr Asp Pro Thr Tyr Gly Ser Ala Leu Gly 
    290                 295                 300                 


Ile Ala Trp Ala Ser Asn Trp Glu Tyr Ser Ala Phe Val Pro Thr Asn 
305                 310                 315                 320 


Pro Trp Arg Ser Ser Met Ser Leu Val Arg Lys Phe Ser Leu Asn Thr 
                325                 330                 335     


Glu Tyr Gln Ala Asn Pro Glu Thr Glu Leu Ile Asn Leu Lys Ala Glu 
            340                 345                 350         


Pro Ile Leu Asn Ile Ser Lys Ala Gly Pro Trp Ser Arg Met Ala Thr 
        355                 360                 365             


Asn Thr Thr Leu Thr Lys Ala Asn Ser Tyr Asn Val Asp Leu Ser Asn 
    370                 375                 380                 


Ser Thr Gly Thr Leu Glu Phe Glu Leu Val Tyr Ala Val Asn Thr Thr 
385                 390                 395                 400 


Gln Thr Ile Ser Lys Ser Val Phe Ala Asp Leu Ser Leu Trp Phe Lys 
                405                 410                 415     


Gly Leu Glu Asp Pro Glu Glu Tyr Leu Arg Met Gly Phe Glu Val Ser 
            420                 425                 430         


Ala Ser Ser Phe Phe Leu Asp Arg Gly Asn Ser Lys Val Lys Phe Val 
        435                 440                 445             


Lys Glu Asn Pro Tyr Phe Thr Asn Arg Met Ser Val Asn Asn Gln Pro 
    450                 455                 460                 


Phe Lys Ser Glu Asn Asp Leu Ser Tyr Tyr Lys Val Tyr Gly Leu Leu 
465                 470                 475                 480 


Asp Gln Asn Ile Leu Glu Leu Tyr Phe Asn Asp Gly Asp Val Val Ser 
                485                 490                 495     


Thr Asn Thr Tyr Phe Met Thr Thr Gly Asn Ala Leu Gly Ser Val Asn 
            500                 505                 510         


Met Thr Thr Gly Val Asp Asn Leu Phe Tyr Ile Asp Lys Phe Gln Val 
        515                 520                 525             


Arg Glu Val Lys 
    530         


<210>  10
<211>  6797
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of construct pSZ6917

<400>  10
agcggaagag cgcccaatgt ttaaacagcc cgcaccctcg ttgatctggg agccctgcgc       60

agccccttaa atcatctcag tcaggtttct gtgttcaact gagcctaaag ggctttcgtc      120

atgcgcacga gcacacgtat atcggccacg cagtttctca aaagcggtag aacagttcgc      180

gagccctcgt aggtcgaaaa cttgcgccag tactattaaa ttaaattaat tgatcgaacg      240

agacgcgaaa cttttgcaga atgccaccga gtttgcccag agaatgggag tggcgccatt      300

caccatccgc ctgtgcccgg cttgattcgc cgagacgatg gacggcgaga ccagggagcg      360

gcttgcgagc cccgagccgg tagcaggaac aatgatcgac aatcttcctg tccaattact      420

ggcaaccatt agaaagagcc ggagcgcgtt gaaagtctgc aatcgagtaa tttttcgata      480

cgtcgggcct gctgaaccct aaggctccgg actttgttta aggcgatcca agatgcacgc      540

ggccccaggc acgtatctca agcacaaacc ccagccttag tttcgagact ttgggagata      600

gcgaccgata tctagtttgg cattttgtat attaattacc tcaagcaatg gagcgctctg      660

atgcggtgca gcgtcggctg cagcacctgg cagtggcgct agggtcgccc tatcgctcgg      720

aacctggtca gctggctccc gcctcctgct cagcctcttc cggtaccctt tcttgcgcta      780

tgacacttcc agcaaaaggt agggcgggct gcgagacggc ttcccggcgc tgcatgcaac      840

accgatgatg cttcgacccc ccgaagctcc ttcggggctg catgggcgct ccgatgccgc      900

tccagggcga gcgctgttta aatagccagg cccccgattg caaagacatt atagcgagct      960

accaaagcca tattcaaaca cctagatcac taccacttct acacaggcca ctcgagcttg     1020

tgatcgcact ccgctaaggg ggcgcctctt cctcttcgtt tcagtcacaa cccgcaaact     1080

ctagaatatc aatgctgctg caggccttcc tgttcctgct ggccggcttc gccgccaagt     1140

gcagcgcctc catgacgaac gagacgtccg acgtgcccct ggtgcacttc acccccaaca     1200

agggctggat gaacgacccc aacggcctgt ggtacgacga gaaggacgcc aagtggcacc     1260

tgtacttcca gtacaacccg aacgacaccg tctgggggac gcccttgttc tggggccacg     1320

ccacgtccga cgacctgacc aactgggagg accagcccat cgccatcgcc ccgaagcgca     1380

acgactccgg cgccttctcc ggctccatgg tggtggacta caacaacacc tccggcttct     1440

tcaacgacac catcgacccg cgccagcgct gcgtggccat ctggacctac aacaccccgg     1500

agtccgagga gcagtacatc tcctacagcc tggacggcgg ctacaccttc accgagtacc     1560

agaagaaccc cgtgctggcc gccaactcca cccagttccg cgacccgaag gtcttctggt     1620

acgagccctc ccagaagtgg atcatgaccg cggccaagtc ccaggactac aagatcgaga     1680

tctactcctc cgacgacctg aagtcctgga agctggagtc cgcgttcgcc aacgagggct     1740

tcctcggcta ccagtacgag tgccccggcc tgatcgaggt ccccaccgag caggacccca     1800

gcaagtccta ctgggtgatg ttcatctcca tcaaccccgg cgccccggcc ggcggctcct     1860

tcaaccagta cttcgtcggc agcttcaacg gcacccactt cgaggccttc gacaaccagt     1920

cccgcgtggt ggacttcggc aaggactact acgccctgca gaccttcttc aacaccgacc     1980

cgacctacgg gagcgccctg ggcatcgcgt gggcctccaa ctgggagtac tccgccttcg     2040

tgcccaccaa cccctggcgc tcctccatgt ccctcgtgcg caagttctcc ctcaacaccg     2100

agtaccaggc caacccggag acggagctga tcaacctgaa ggccgagccg atcctgaaca     2160

tcagcaaggc cggcccctgg agccggatgg ccaccaacac cacgttgacg aaggccaaca     2220

gctacaacgt cgacctgtcc aacagcaccg gcaccctgga gttcgagctg gtgtacgccg     2280

tcaacaccac ccagacgatc tccaagtccg tgttcgcgga cctctccctc tggttcaagg     2340

gcctggagga ccccgaggag tacctccgca tgggcttcga ggtgtccgcg tcctccttct     2400

tcctggaccg cgggaacagc aaggtgaagt tcgtgaagga gaacccctac ttcaccaacc     2460

gcatgagcgt gaacaaccag cccttcaaga gcgagaacga cctgtcctac tacaaggtgt     2520

acggcttgct ggaccagaac atcctggagc tgtacttcaa cgacggcgac gtcgtgtcca     2580

ccaacaccta cttcatgacc accgggaacg ccctgggctc cgtgaacatg acgacggggg     2640

tggacaacct gttctacatc gacaagttcc aggtgcgcga ggtcaagtga caattgacgc     2700

ccgcgcggcg cacctgacct gttctctcga gggcgcctgt tctgccttgc gaaacaagcc     2760

cctggagcat gcgtgcatga tcgtctctgg cgccccgccg cgcggtttgt cgccctcgcg     2820

ggcgccgcgg ccgcgggggc gcattgaaat tgttgcaaac cccacctgac agattgaggg     2880

cccaggcagg aaggcgttga gatggaggta caggagtcaa gtaactgaaa gtttttatga     2940

taactaacaa caaagggtcg tttctggcca gcgaatgaca agaacaagat tccacatttc     3000

cgtgtagagg cttgccatcg aatgtgagcg ggcgggccgc ggacccgaca aaacccttac     3060

gacgtggtaa gaaaaacgtg gcgggcactg tccctgtagc ctgaagacca gcaggagacg     3120

atcggaagca tcacagcaca ggatcccgcg tctcgaacag agcgcgcaga ggaacgctga     3180

aggtctcgcc tctgtcgcac ctcagcgcgg catacaccac aataaccacc tgacgaatgc     3240

gcttggttct tcgtccatta gcgaagcgtc cggttcacac acgtgccacg ttggcgaggt     3300

ggcaggtgac aatgatcggt ggagctgatg gtcgaaacgt tcacagccta ggatcgagtg     3360

tacagtcaat gaatggtgag ctcagcgtct gcgtgttggg agctggagtc gtgggcttga     3420

cgacggcgct gcagctgttg caggatgtgc ctggcgtgcg cgttcacgtc gtggctgaga     3480

aatatggcga cgaaacgttg acggctgggg ccggcgggct gtggatgcca tacgcattgg     3540

gtacgcggcc attggatggg attgataggc ttatggaggg ataatagagt ttttgccgga     3600

tccaacgcat gtggatgcgg tatcccggtg ggctgaaagt gtggaaggat agtgcattgg     3660

ctattcacat gcactgccca ccccttttgg caggaaatgt gccggcatcg ttggtgcacc     3720

gatggggaaa atcgacgttc gaccactaca tgaagattta tacgtctgaa gatgcagcga     3780

ctgcgggtgc gaaacggatg acggtttggt cgtgtatgtc acagcatgtg ctggatcttg     3840

cgggctaact ccccctgcca cggcccattg caggtgtcat gttgactgga gggtacgacc     3900

tttcgtccgt caaattccca gaggaggacc cgctctgggc cgacattgtg cccactgaag     3960

agcgtttaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag     4020

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca     4080

ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag     4140

cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa     4200

ccctcactaa agggaacaaa agctggccaa ttcgccctat agtgagtcgt attacaattc     4260

actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg     4320

ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg     4380

cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt     4440

aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc     4500

gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca     4560

agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc     4620

caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt     4680

tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac     4740

aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc     4800

ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt     4860

aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta     4920

tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt     4980

caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc     5040

ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa     5100

gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt     5160

aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt     5220

ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc     5280

atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg     5340

gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg     5400

gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac     5460

atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca     5520

aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta     5580

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat     5640

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa     5700

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag     5760

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat     5820

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt     5880

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg     5940

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga     6000

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta     6060

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa     6120

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact     6180

gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca     6240

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt     6300

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg     6360

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag     6420

cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta     6480

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat     6540

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg     6600

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc     6660

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac     6720

cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc     6780

gagtcagtga gcgagga                                                    6797


<210>  11
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wild-type S. cerevisiae SUC2 with codon bias for improved 
       expression in P. moriformis

<400>  11
atgctgctgc aggccttcct gttcctgctg gccggcttcg ccgccaagat cagcgcctcc       60

atgacgaacg agacgtccga ccgccccctg gtgcacttca cccccaacaa gggctggatg      120

aacgacccca acggcctgtg gtacgacgag aaggacgcca agtggcacct gtacttccag      180

tacaacccga acgacaccgt ctgggggacg cccttgttct ggggccacgc cacgtccgac      240

gacctgacca actgggagga ccagcccatc gccatcgccc cgaagcgcaa cgactccggc      300

gccttctccg gctccatggt ggtggactac aacaacacct ccggcttctt caacgacacc      360

atcgacccgc gccagcgctg cgtggccatc tggacctaca acaccccgga gtccgaggag      420

cagtacatct cctacagcct ggacggcggc tacaccttca ccgagtacca gaagaacccc      480

gtgctggccg ccaactccac ccagttccgc gacccgaagg tcttctggta cgagccctcc      540

cagaagtgga tcatgaccgc ggccaagtcc caggactaca agatcgagat ctactcctcc      600

gacgacctga agtcctggaa gctggagtcc gcgttcgcca acgagggctt cctcggctac      660

cagtacgagt gccccggcct gatcgaggtc cccaccgagc aggaccccag caagtcctac      720

tgggtgatgt tcatctccat caaccccggc gccccggccg gcggctcctt caaccagtac      780

ttcgtcggca gcttcaacgg cacccacttc gaggccttcg acaaccagtc ccgcgtggtg      840

gacttcggca aggactacta cgccctgcag accttcttca acaccgaccc gacctacggg      900

agcgccctgg gcatcgcgtg ggcctccaac tgggagtact ccgccttcgt gcccaccaac      960

ccctggcgct cctccatgtc cctcgtgcgc aagttctccc tcaacaccga gtaccaggcc     1020

aacccggaga cggagctgat caacctgaag gccgagccga tcctgaacat cagcaacgcc     1080

ggcccctgga gccggttcgc caccaacacc acgttgacga aggccaacag ctacaacgtc     1140

gacctgtcca acagcaccgg caccctggag ttcgagctgg tgtacgccgt caacaccacc     1200

cagacgatct ccaagtccgt gttcgcggac ctctccctct ggttcaaggg cctggaggac     1260

cccgaggagt acctccgcat gggcttcgag gtgtccgcgt cctccttctt cctggaccgc     1320

gggaacagca aggtgaagtt cgtgaaggag aacccctact tcaccaaccg catgagcgtg     1380

aacaaccagc ccttcaagag cgagaacgac ctgtcctact acaaggtgta cggcttgctg     1440

gaccagaaca tcctggagct gtacttcaac gacggcgacg tcgtgtccac caacacctac     1500

ttcatgacca ccgggaacgc cctgggctcc gtgaacatga cgacgggggt ggacaacctg     1560

ttctacatcg acaagttcca ggtgcgcgag gtcaagtga                            1599


<210>  12
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae SUC2 I17C enzyme variant

<400>  12
atgctgctgc aggccttcct gttcctgctg gccggcttcg ccgccaagtg cagcgcctcc       60

atgacgaacg agacgtccga ccgccccctg gtgcacttca cccccaacaa gggctggatg      120

aacgacccca acggcctgtg gtacgacgag aaggacgcca agtggcacct gtacttccag      180

tacaacccga acgacaccgt ctgggggacg cccttgttct ggggccacgc cacgtccgac      240

gacctgacca actgggagga ccagcccatc gccatcgccc cgaagcgcaa cgactccggc      300

gccttctccg gctccatggt ggtggactac aacaacacct ccggcttctt caacgacacc      360

atcgacccgc gccagcgctg cgtggccatc tggacctaca acaccccgga gtccgaggag      420

cagtacatct cctacagcct ggacggcggc tacaccttca ccgagtacca gaagaacccc      480

gtgctggccg ccaactccac ccagttccgc gacccgaagg tcttctggta cgagccctcc      540

cagaagtgga tcatgaccgc ggccaagtcc caggactaca agatcgagat ctactcctcc      600

gacgacctga agtcctggaa gctggagtcc gcgttcgcca acgagggctt cctcggctac      660

cagtacgagt gccccggcct gatcgaggtc cccaccgagc aggaccccag caagtcctac      720

tgggtgatgt tcatctccat caaccccggc gccccggccg gcggctcctt caaccagtac      780

ttcgtcggca gcttcaacgg cacccacttc gaggccttcg acaaccagtc ccgcgtggtg      840

gacttcggca aggactacta cgccctgcag accttcttca acaccgaccc gacctacggg      900

agcgccctgg gcatcgcgtg ggcctccaac tgggagtact ccgccttcgt gcccaccaac      960

ccctggcgct cctccatgtc cctcgtgcgc aagttctccc tcaacaccga gtaccaggcc     1020

aacccggaga cggagctgat caacctgaag gccgagccga tcctgaacat cagcaacgcc     1080

ggcccctgga gccggttcgc caccaacacc acgttgacga aggccaacag ctacaacgtc     1140

gacctgtcca acagcaccgg caccctggag ttcgagctgg tgtacgccgt caacaccacc     1200

cagacgatct ccaagtccgt gttcgcggac ctctccctct ggttcaaggg cctggaggac     1260

cccgaggagt acctccgcat gggcttcgag gtgtccgcgt cctccttctt cctggaccgc     1320

gggaacagca aggtgaagtt cgtgaaggag aacccctact tcaccaaccg catgagcgtg     1380

aacaaccagc ccttcaagag cgagaacgac ctgtcctact acaaggtgta cggcttgctg     1440

gaccagaaca tcctggagct gtacttcaac gacggcgacg tcgtgtccac caacacctac     1500

ttcatgacca ccgggaacgc cctgggctcc gtgaacatga cgacgggggt ggacaacctg     1560

ttctacatcg acaagttcca ggtgcgcgag gtcaagtga                            1599


<210>  13
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae SUC2 R28V enzyme variant

<400>  13
atgctgctgc aggccttcct gttcctgctg gccggcttcg ccgccaagat cagcgcctcc       60

atgacgaacg agacgtccga cgtgcccctg gtgcacttca cccccaacaa gggctggatg      120

aacgacccca acggcctgtg gtacgacgag aaggacgcca agtggcacct gtacttccag      180

tacaacccga acgacaccgt ctgggggacg cccttgttct ggggccacgc cacgtccgac      240

gacctgacca actgggagga ccagcccatc gccatcgccc cgaagcgcaa cgactccggc      300

gccttctccg gctccatggt ggtggactac aacaacacct ccggcttctt caacgacacc      360

atcgacccgc gccagcgctg cgtggccatc tggacctaca acaccccgga gtccgaggag      420

cagtacatct cctacagcct ggacggcggc tacaccttca ccgagtacca gaagaacccc      480

gtgctggccg ccaactccac ccagttccgc gacccgaagg tcttctggta cgagccctcc      540

cagaagtgga tcatgaccgc ggccaagtcc caggactaca agatcgagat ctactcctcc      600

gacgacctga agtcctggaa gctggagtcc gcgttcgcca acgagggctt cctcggctac      660

cagtacgagt gccccggcct gatcgaggtc cccaccgagc aggaccccag caagtcctac      720

tgggtgatgt tcatctccat caaccccggc gccccggccg gcggctcctt caaccagtac      780

ttcgtcggca gcttcaacgg cacccacttc gaggccttcg acaaccagtc ccgcgtggtg      840

gacttcggca aggactacta cgccctgcag accttcttca acaccgaccc gacctacggg      900

agcgccctgg gcatcgcgtg ggcctccaac tgggagtact ccgccttcgt gcccaccaac      960

ccctggcgct cctccatgtc cctcgtgcgc aagttctccc tcaacaccga gtaccaggcc     1020

aacccggaga cggagctgat caacctgaag gccgagccga tcctgaacat cagcaacgcc     1080

ggcccctgga gccggttcgc caccaacacc acgttgacga aggccaacag ctacaacgtc     1140

gacctgtcca acagcaccgg caccctggag ttcgagctgg tgtacgccgt caacaccacc     1200

cagacgatct ccaagtccgt gttcgcggac ctctccctct ggttcaaggg cctggaggac     1260

cccgaggagt acctccgcat gggcttcgag gtgtccgcgt cctccttctt cctggaccgc     1320

gggaacagca aggtgaagtt cgtgaaggag aacccctact tcaccaaccg catgagcgtg     1380

aacaaccagc ccttcaagag cgagaacgac ctgtcctact acaaggtgta cggcttgctg     1440

gaccagaaca tcctggagct gtacttcaac gacggcgacg tcgtgtccac caacacctac     1500

ttcatgacca ccgggaacgc cctgggctcc gtgaacatga cgacgggggt ggacaacctg     1560

ttctacatcg acaagttcca ggtgcgcgag gtcaagtga                            1599


<210>  14
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae SUC2 I17C-R28V enzyme variant

<400>  14
atgctgctgc aggccttcct gttcctgctg gccggcttcg ccgccaagtg cagcgcctcc       60

atgacgaacg agacgtccga cgtgcccctg gtgcacttca cccccaacaa gggctggatg      120

aacgacccca acggcctgtg gtacgacgag aaggacgcca agtggcacct gtacttccag      180

tacaacccga acgacaccgt ctgggggacg cccttgttct ggggccacgc cacgtccgac      240

gacctgacca actgggagga ccagcccatc gccatcgccc cgaagcgcaa cgactccggc      300

gccttctccg gctccatggt ggtggactac aacaacacct ccggcttctt caacgacacc      360

atcgacccgc gccagcgctg cgtggccatc tggacctaca acaccccgga gtccgaggag      420

cagtacatct cctacagcct ggacggcggc tacaccttca ccgagtacca gaagaacccc      480

gtgctggccg ccaactccac ccagttccgc gacccgaagg tcttctggta cgagccctcc      540

cagaagtgga tcatgaccgc ggccaagtcc caggactaca agatcgagat ctactcctcc      600

gacgacctga agtcctggaa gctggagtcc gcgttcgcca acgagggctt cctcggctac      660

cagtacgagt gccccggcct gatcgaggtc cccaccgagc aggaccccag caagtcctac      720

tgggtgatgt tcatctccat caaccccggc gccccggccg gcggctcctt caaccagtac      780

ttcgtcggca gcttcaacgg cacccacttc gaggccttcg acaaccagtc ccgcgtggtg      840

gacttcggca aggactacta cgccctgcag accttcttca acaccgaccc gacctacggg      900

agcgccctgg gcatcgcgtg ggcctccaac tgggagtact ccgccttcgt gcccaccaac      960

ccctggcgct cctccatgtc cctcgtgcgc aagttctccc tcaacaccga gtaccaggcc     1020

aacccggaga cggagctgat caacctgaag gccgagccga tcctgaacat cagcaacgcc     1080

ggcccctgga gccggttcgc caccaacacc acgttgacga aggccaacag ctacaacgtc     1140

gacctgtcca acagcaccgg caccctggag ttcgagctgg tgtacgccgt caacaccacc     1200

cagacgatct ccaagtccgt gttcgcggac ctctccctct ggttcaaggg cctggaggac     1260

cccgaggagt acctccgcat gggcttcgag gtgtccgcgt cctccttctt cctggaccgc     1320

gggaacagca aggtgaagtt cgtgaaggag aacccctact tcaccaaccg catgagcgtg     1380

aacaaccagc ccttcaagag cgagaacgac ctgtcctact acaaggtgta cggcttgctg     1440

gaccagaaca tcctggagct gtacttcaac gacggcgacg tcgtgtccac caacacctac     1500

ttcatgacca ccgggaacgc cctgggctcc gtgaacatga cgacgggggt ggacaacctg     1560

ttctacatcg acaagttcca ggtgcgcgag gtcaagtga                            1599


<210>  15
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae SUC2 I17C-R28V-N359K enzyme variant

<400>  15
atgctgctgc aggccttcct gttcctgctg gccggcttcg ccgccaagtg cagcgcctcc       60

atgacgaacg agacgtccga cgtgcccctg gtgcacttca cccccaacaa gggctggatg      120

aacgacccca acggcctgtg gtacgacgag aaggacgcca agtggcacct gtacttccag      180

tacaacccga acgacaccgt ctgggggacg cccttgttct ggggccacgc cacgtccgac      240

gacctgacca actgggagga ccagcccatc gccatcgccc cgaagcgcaa cgactccggc      300

gccttctccg gctccatggt ggtggactac aacaacacct ccggcttctt caacgacacc      360

atcgacccgc gccagcgctg cgtggccatc tggacctaca acaccccgga gtccgaggag      420

cagtacatct cctacagcct ggacggcggc tacaccttca ccgagtacca gaagaacccc      480

gtgctggccg ccaactccac ccagttccgc gacccgaagg tcttctggta cgagccctcc      540

cagaagtgga tcatgaccgc ggccaagtcc caggactaca agatcgagat ctactcctcc      600

gacgacctga agtcctggaa gctggagtcc gcgttcgcca acgagggctt cctcggctac      660

cagtacgagt gccccggcct gatcgaggtc cccaccgagc aggaccccag caagtcctac      720

tgggtgatgt tcatctccat caaccccggc gccccggccg gcggctcctt caaccagtac      780

ttcgtcggca gcttcaacgg cacccacttc gaggccttcg acaaccagtc ccgcgtggtg      840

gacttcggca aggactacta cgccctgcag accttcttca acaccgaccc gacctacggg      900

agcgccctgg gcatcgcgtg ggcctccaac tgggagtact ccgccttcgt gcccaccaac      960

ccctggcgct cctccatgtc cctcgtgcgc aagttctccc tcaacaccga gtaccaggcc     1020

aacccggaga cggagctgat caacctgaag gccgagccga tcctgaacat cagcaaggcc     1080

ggcccctgga gccggttcgc caccaacacc acgttgacga aggccaacag ctacaacgtc     1140

gacctgtcca acagcaccgg caccctggag ttcgagctgg tgtacgccgt caacaccacc     1200

cagacgatct ccaagtccgt gttcgcggac ctctccctct ggttcaaggg cctggaggac     1260

cccgaggagt acctccgcat gggcttcgag gtgtccgcgt cctccttctt cctggaccgc     1320

gggaacagca aggtgaagtt cgtgaaggag aacccctact tcaccaaccg catgagcgtg     1380

aacaaccagc ccttcaagag cgagaacgac ctgtcctact acaaggtgta cggcttgctg     1440

gaccagaaca tcctggagct gtacttcaac gacggcgacg tcgtgtccac caacacctac     1500

ttcatgacca ccgggaacgc cctgggctcc gtgaacatga cgacgggggt ggacaacctg     1560

ttctacatcg acaagttcca ggtgcgcgag gtcaagtga                            1599


<210>  16
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae SUC2 I17C-R28V-F366M enzyme variant

<400>  16
atgctgctgc aggccttcct gttcctgctg gccggcttcg ccgccaagtg cagcgcctcc       60

atgacgaacg agacgtccga cgtgcccctg gtgcacttca cccccaacaa gggctggatg      120

aacgacccca acggcctgtg gtacgacgag aaggacgcca agtggcacct gtacttccag      180

tacaacccga acgacaccgt ctgggggacg cccttgttct ggggccacgc cacgtccgac      240

gacctgacca actgggagga ccagcccatc gccatcgccc cgaagcgcaa cgactccggc      300

gccttctccg gctccatggt ggtggactac aacaacacct ccggcttctt caacgacacc      360

atcgacccgc gccagcgctg cgtggccatc tggacctaca acaccccgga gtccgaggag      420

cagtacatct cctacagcct ggacggcggc tacaccttca ccgagtacca gaagaacccc      480

gtgctggccg ccaactccac ccagttccgc gacccgaagg tcttctggta cgagccctcc      540

cagaagtgga tcatgaccgc ggccaagtcc caggactaca agatcgagat ctactcctcc      600

gacgacctga agtcctggaa gctggagtcc gcgttcgcca acgagggctt cctcggctac      660

cagtacgagt gccccggcct gatcgaggtc cccaccgagc aggaccccag caagtcctac      720

tgggtgatgt tcatctccat caaccccggc gccccggccg gcggctcctt caaccagtac      780

ttcgtcggca gcttcaacgg cacccacttc gaggccttcg acaaccagtc ccgcgtggtg      840

gacttcggca aggactacta cgccctgcag accttcttca acaccgaccc gacctacggg      900

agcgccctgg gcatcgcgtg ggcctccaac tgggagtact ccgccttcgt gcccaccaac      960

ccctggcgct cctccatgtc cctcgtgcgc aagttctccc tcaacaccga gtaccaggcc     1020

aacccggaga cggagctgat caacctgaag gccgagccga tcctgaacat cagcaacgcc     1080

ggcccctgga gccggatggc caccaacacc acgttgacga aggccaacag ctacaacgtc     1140

gacctgtcca acagcaccgg caccctggag ttcgagctgg tgtacgccgt caacaccacc     1200

cagacgatct ccaagtccgt gttcgcggac ctctccctct ggttcaaggg cctggaggac     1260

cccgaggagt acctccgcat gggcttcgag gtgtccgcgt cctccttctt cctggaccgc     1320

gggaacagca aggtgaagtt cgtgaaggag aacccctact tcaccaaccg catgagcgtg     1380

aacaaccagc ccttcaagag cgagaacgac ctgtcctact acaaggtgta cggcttgctg     1440

gaccagaaca tcctggagct gtacttcaac gacggcgacg tcgtgtccac caacacctac     1500

ttcatgacca ccgggaacgc cctgggctcc gtgaacatga cgacgggggt ggacaacctg     1560

ttctacatcg acaagttcca ggtgcgcgag gtcaagtga                            1599


<210>  17
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae SUC2 I17C-R28V-N359K-F366M enzyme variant

<400>  17
atgctgctgc aggccttcct gttcctgctg gccggcttcg ccgccaagtg cagcgcctcc       60

atgacgaacg agacgtccga cgtgcccctg gtgcacttca cccccaacaa gggctggatg      120

aacgacccca acggcctgtg gtacgacgag aaggacgcca agtggcacct gtacttccag      180

tacaacccga acgacaccgt ctgggggacg cccttgttct ggggccacgc cacgtccgac      240

gacctgacca actgggagga ccagcccatc gccatcgccc cgaagcgcaa cgactccggc      300

gccttctccg gctccatggt ggtggactac aacaacacct ccggcttctt caacgacacc      360

atcgacccgc gccagcgctg cgtggccatc tggacctaca acaccccgga gtccgaggag      420

cagtacatct cctacagcct ggacggcggc tacaccttca ccgagtacca gaagaacccc      480

gtgctggccg ccaactccac ccagttccgc gacccgaagg tcttctggta cgagccctcc      540

cagaagtgga tcatgaccgc ggccaagtcc caggactaca agatcgagat ctactcctcc      600

gacgacctga agtcctggaa gctggagtcc gcgttcgcca acgagggctt cctcggctac      660

cagtacgagt gccccggcct gatcgaggtc cccaccgagc aggaccccag caagtcctac      720

tgggtgatgt tcatctccat caaccccggc gccccggccg gcggctcctt caaccagtac      780

ttcgtcggca gcttcaacgg cacccacttc gaggccttcg acaaccagtc ccgcgtggtg      840

gacttcggca aggactacta cgccctgcag accttcttca acaccgaccc gacctacggg      900

agcgccctgg gcatcgcgtg ggcctccaac tgggagtact ccgccttcgt gcccaccaac      960

ccctggcgct cctccatgtc cctcgtgcgc aagttctccc tcaacaccga gtaccaggcc     1020

aacccggaga cggagctgat caacctgaag gccgagccga tcctgaacat cagcaaggcc     1080

ggcccctgga gccggatggc caccaacacc acgttgacga aggccaacag ctacaacgtc     1140

gacctgtcca acagcaccgg caccctggag ttcgagctgg tgtacgccgt caacaccacc     1200

cagacgatct ccaagtccgt gttcgcggac ctctccctct ggttcaaggg cctggaggac     1260

cccgaggagt acctccgcat gggcttcgag gtgtccgcgt cctccttctt cctggaccgc     1320

gggaacagca aggtgaagtt cgtgaaggag aacccctact tcaccaaccg catgagcgtg     1380

aacaaccagc ccttcaagag cgagaacgac ctgtcctact acaaggtgta cggcttgctg     1440

gaccagaaca tcctggagct gtacttcaac gacggcgacg tcgtgtccac caacacctac     1500

ttcatgacca ccgggaacgc cctgggctcc gtgaacatga cgacgggggt ggacaacctg     1560

ttctacatcg acaagttcca ggtgcgcgag gtcaagtga                            1599


<210>  18
<211>  1599
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  18
atgcttttgc aagctttcct tttccttttg gctggttttg cagccaaaat atctgcatca       60

atgacaaacg aaactagcga tagacctttg gtccacttca cacccaacaa gggctggatg      120

aatgacccaa atgggttgtg gtacgatgaa aaagatgcca aatggcatct gtactttcaa      180

tacaacccaa atgacaccgt atggggtacg ccattgtttt ggggccatgc tacttccgat      240

gatttgacta attgggaaga tcaacccatt gctatcgctc ccaagcgtaa cgattcaggt      300

gctttctctg gctccatggt ggttgattac aacaacacga gtgggttttt caatgatact      360

attgatccaa gacaaagatg cgttgcgatt tggacttata acactcctga aagtgaagag      420

caatacatta gctattctct tgatggtggt tacactttta ctgaatacca aaagaaccct      480

gttttagctg ccaactccac tcaattcaga gatccaaagg tgttctggta tgaaccttct      540

caaaaatgga ttatgacggc tgccaaatca caagactaca aaattgaaat ttactcctct      600

gatgacttga agtcctggaa gctagaatct gcatttgcca atgaaggttt cttaggctac      660

caatacgaat gtccaggttt gattgaagtc ccaactgagc aagatccttc caaatcttat      720

tgggtcatgt ttatttctat caacccaggt gcacctgctg gcggttcctt caaccaatat      780

tttgttggat ccttcaatgg tactcatttt gaagcgtttg acaatcaatc tagagtggta      840

gattttggta aggactacta tgccttgcaa actttcttca acactgaccc aacctacggt      900

tcagcattag gtattgcctg ggcttcaaac tgggagtaca gtgcctttgt cccaactaac      960

ccatggagat catccatgtc tttggtccgc aagttttctt tgaacactga atatcaagct     1020

aatccagaga ctgaattgat caatttgaaa gccgaaccaa tattgaacat tagtaatgct     1080

ggtccctggt ctcgttttgc tactaacaca actctaacta aggccaattc ttacaatgtc     1140

gatttgagca actcgactgg taccctagag tttgagttgg tttacgctgt taacaccaca     1200

caaaccatat ccaaatccgt ctttgccgac ttatcacttt ggttcaaggg tttagaagat     1260

cctgaagaat atttgagaat gggttttgaa gtcagtgctt cttccttctt tttggaccgt     1320

ggtaactcta aggtcaagtt tgtcaaggag aacccatatt tcacaaacag aatgtctgtc     1380

aacaaccaac cattcaagtc tgagaacgac ctaagttact ataaagtgta cggcctactg     1440

gatcaaaaca tcttggaatt gtacttcaac gatggagatg tggtttctac aaatacctac     1500

ttcatgacca ccggtaacgc tctaggatct gtgaacatga ccactggtgt cgataatttg     1560

ttctacattg acaagttcca agtaagggaa gtaaaatag                            1599


