                         SEQUENCE LISTING

<110>  The Trustees of Columbia University in the City of New York
 
<120>  CRISPR-BASED METHODS FOR RECORDING BIOLOGICAL SIGNALS

<130>  COLUM-38841_601

<150>  US 62/937,029
<151>  2019-11-18

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  305
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Thr Trp Leu Pro Leu Asn Pro Ile Pro Leu Lys Asp Arg Val Ser 
1               5                   10                  15      


Met Ile Phe Leu Gln Tyr Gly Gln Ile Asp Val Ile Asp Gly Ala Phe 
            20                  25                  30          


Val Leu Ile Asp Lys Thr Gly Ile Arg Thr His Ile Pro Val Gly Ser 
        35                  40                  45              


Val Ala Cys Ile Met Leu Glu Pro Gly Thr Arg Val Ser His Ala Ala 
    50                  55                  60                  


Val Arg Leu Ala Ala Gln Val Gly Thr Leu Leu Val Trp Val Gly Glu 
65                  70                  75                  80  


Ala Gly Val Arg Val Tyr Ala Ser Gly Gln Pro Gly Gly Ala Arg Ser 
                85                  90                  95      


Asp Lys Leu Leu Tyr Gln Ala Lys Leu Ala Leu Asp Glu Asp Leu Arg 
            100                 105                 110         


Leu Lys Val Val Arg Lys Met Phe Glu Leu Arg Phe Gly Glu Pro Ala 
        115                 120                 125             


Pro Ala Arg Arg Ser Val Glu Gln Leu Arg Gly Ile Glu Gly Ser Arg 
    130                 135                 140                 


Val Arg Ala Thr Tyr Ala Leu Leu Ala Lys Gln Tyr Gly Val Thr Trp 
145                 150                 155                 160 


Asn Gly Arg Arg Tyr Asp Pro Lys Asp Trp Glu Lys Gly Asp Thr Ile 
                165                 170                 175     


Asn Gln Cys Ile Ser Ala Ala Thr Ser Cys Leu Tyr Gly Val Thr Glu 
            180                 185                 190         


Ala Ala Ile Leu Ala Ala Gly Tyr Ala Pro Ala Ile Gly Phe Val His 
        195                 200                 205             


Thr Gly Lys Pro Leu Ser Phe Val Tyr Asp Ile Ala Asp Ile Ile Lys 
    210                 215                 220                 


Phe Asp Thr Val Val Pro Lys Ala Phe Glu Ile Ala Arg Arg Asn Pro 
225                 230                 235                 240 


Gly Glu Pro Asp Arg Glu Val Arg Leu Ala Cys Arg Asp Ile Phe Arg 
                245                 250                 255     


Ser Ser Lys Thr Leu Ala Lys Leu Ile Pro Leu Ile Glu Asp Val Leu 
            260                 265                 270         


Ala Ala Gly Glu Ile Gln Pro Pro Ala Pro Pro Glu Asp Ala Gln Pro 
        275                 280                 285             


Val Ala Ile Pro Leu Pro Val Ser Leu Gly Asp Ala Gly His Arg Ser 
    290                 295                 300                 


Ser 
305 


<210>  2
<211>  94
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Ser Met Leu Val Val Val Thr Glu Asn Val Pro Pro Arg Leu Arg 
1               5                   10                  15      


Gly Arg Leu Ala Ile Trp Leu Leu Glu Val Arg Ala Gly Val Tyr Val 
            20                  25                  30          


Gly Asp Val Ser Ala Lys Ile Arg Glu Met Ile Trp Glu Gln Ile Ala 
        35                  40                  45              


Gly Leu Ala Glu Glu Gly Asn Val Val Met Ala Trp Ala Thr Asn Thr 
    50                  55                  60                  


Glu Thr Gly Phe Glu Phe Gln Thr Phe Gly Leu Asn Arg Arg Thr Pro 
65                  70                  75                  80  


Val Asp Leu Asp Gly Leu Arg Leu Val Ser Phe Leu Pro Val 
                85                  90                  


<210>  3
<211>  90
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  3
ggaagagagt caattcaggg tggtgaatgt gaaaccagta tagtgataag atgtcgcaga       60

gtatgccggt gtctcttatc agaccgtttc                                        90


<210>  4
<211>  90
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (42)..(49)
<223>  n is a, c, g, or t

<400>  4
ggggaacacc cgtaagtggt ttgagcgatg atatttgtgc tnnnnnnnnc cccgctggcg       60

cggggaacac tctaaacata acctattatt                                        90


<210>  5
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  5
gcgagcgatc cagaagatct                                                   20


<210>  6
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  6
gggtaaagga tgccacagac a                                                 21


<210>  7
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  7
cgctctatga tccagtcgat tt                                                22


<210>  8
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  8
tccgtatgcc atgcgtttat                                                   20


<210>  9
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  9
aatgatacgg cgaccaccga gatctacact agatcgcctg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  10
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  10
aatgatacgg cgaccaccga gatctacacc tctctatctg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  11
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  11
aatgatacgg cgaccaccga gatctacact atcctctctg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  12
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  12
aatgatacgg cgaccaccga gatctacaca gagtagactg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  13
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  13
aatgatacgg cgaccaccga gatctacacg taaggagctg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  14
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  14
aatgatacgg cgaccaccga gatctacaca ctgcatactg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  15
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  15
aatgatacgg cgaccaccga gatctacaca aggagtactg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  16
<211>  79
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  16
aatgatacgg cgaccaccga gatctacacc taagcctctg gcttaaaaaa tcattaatta       60

ataataggtt atgtttaga                                                    79


<210>  17
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  17
caagcagaag acggcatacg agattcgcct taggtttgag cgatgatatt tgtgct           56


<210>  18
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  18
caagcagaag acggcatacg agatctagta cgggtttgag cgatgatatt tgtgct           56


<210>  19
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  19
caagcagaag acggcatacg agatttctgc ctggtttgag cgatgatatt tgtgct           56


<210>  20
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  20
caagcagaag acggcatacg agatgctcag gaggtttgag cgatgatatt tgtgct           56


<210>  21
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  21
caagcagaag acggcatacg agataggagt ccggtttgag cgatgatatt tgtgct           56


<210>  22
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  22
caagcagaag acggcatacg agatcatgcc taggtttgag cgatgatatt tgtgct           56


<210>  23
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  23
caagcagaag acggcatacg agatgtagag agggtttgag cgatgatatt tgtgct           56


<210>  24
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  24
caagcagaag acggcatacg agatcctctc tgggtttgag cgatgatatt tgtgct           56


<210>  25
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  25
caagcagaag acggcatacg agatagcgta gcggtttgag cgatgatatt tgtgct           56


<210>  26
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  26
caagcagaag acggcatacg agatcagcct cgggtttgag cgatgatatt tgtgct           56


<210>  27
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  27
caagcagaag acggcatacg agattgcctc ttggtttgag cgatgatatt tgtgct           56


<210>  28
<211>  56
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  28
caagcagaag acggcatacg agattcctct acggtttgag cgatgatatt tgtgct           56


<210>  29
<211>  58
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  29
ctggcttaaa aaatcattaa ttaataatag gttatgttta gagtgttccc cgcgccag         58


<210>  30
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  30
cggggataaa ccgagcacaa atatcatcgc tcaaacc                                37


<210>  31
<211>  285
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  31
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atactacctg acgcttttta tcgcaactct ctactgtttc tccat                      285


<210>  32
<211>  4755
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  32
ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg       60

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc      120

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc      180

ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc      240

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg      300

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc      360

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga      420

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc      480

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac      540

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg      600

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc      660

acgttaaggg attttggtca tgactagtgc ttggattctc accaataaaa aacgcccggc      720

ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca      780

acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac      840

tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc      900

tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa      960

acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc     1020

cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg     1080

ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg     1140

tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa     1200

gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga     1260

tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt     1320

ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat     1380

tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg     1440

gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat     1500

aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt     1560

acgtgccgat caacgtctca ttttcgccag atatcgacgt ctaagaaacc attattatca     1620

tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagtcgg     1680

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     1740

tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tcatcgatgc ataatgtgcc     1800

tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt     1860

gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa     1920

ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag     1980

agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc     2040

aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct     2100

aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg     2160

ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt     2220

acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac     2280

aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg     2340

ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag     2400

aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga     2460

cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga     2520

atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga     2580

tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc     2640

ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc     2700

agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt     2760

ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt     2820

cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc     2880

attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat     2940

cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca     3000

tttttatcca taagattagc ggatactacc tgacgctttt tatcgcaact ctctactgtt     3060

tctccatacc cgtttttttg ggctagaaat aattttgttt aactttaaga aggagatata     3120

catatgcggg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag     3180

caaatgggtc gggatctgta cgagaacctg tacttccagg aggacgcctt atgacctggc     3240

ttccccttaa tcccattcca ctcaaagatc gcgtctccat gatctttctg caatatgggc     3300

agatcgatgt aatagatggc gcgtttgtac ttatcgacaa gacagggatc cgcactcata     3360

ttcctgttgg ctcggttgcc tgcatcatgc tggaacctgg tacacgggtt tcgcatgcag     3420

ctgtacgcct ggctgcgcaa gttggaacat tgttggtatg ggtgggggaa gcgggcgttc     3480

gtgtttatgc ttctggtcag cctggaggtg cgcgttcaga taagctgctc tatcaggcaa     3540

aacttgctct ggatgaagat ttgcgtctga aggtcgtacg taaaatgttt gaacttcggt     3600

ttggagaacc tgcgcctgcc cggcgctccg tagagcaact cagaggtata gaaggcagtc     3660

gcgtgcgggc aacctacgca cttctggcga agcaatacgg cgtgacatgg aatggacgtc     3720

gctacgatcc gaaagactgg gaaaagggcg atacgatcaa ccaatgcatt agcgctgcaa     3780

cttcctgttt atacggcgta actgaagcgg cgatacttgc agctggttat gcaccagcta     3840

ttgggtttgt gcatacagga aagcctcttt cctttgttta cgatattgca gacatcatta     3900

aatttgacac tgttgtaccg aaagcttttg agatagcgcg tcgtaaccct ggtgagccgg     3960

accgggaagt ccgtttggcg tgcagggata tttttcgcag tagtaaaaca ttagccaaat     4020

tgattccgct tatagaggac gtgcttgccg ctggagaaat acaaccgccg gccccacctg     4080

aagatgcaca gcctgttgcc attccgcttc ctgtttcact gggagatgca ggccatcgga     4140

gtagctgaaa tgagtatgtt ggtcgtggtc actgaaaatg tacctccgcg cttacgaggc     4200

agattagcca tctggttgtt ggaggtacgt gcaggggtat atgtaggtga tgtatccgca     4260

aaaattcgtg aaatgatctg ggaacaaata gctggactgg cggaagaagg caatgtagtg     4320

atggcatggg caacgaatac ggaaacggga tttgagttcc agacatttgg gttaaacagg     4380

cgtaccccgg tagatttgga tggtttaagg ttggtgtctt ttttacctgt ttgataataa     4440

tctagaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta     4500

tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc     4560

ctttctgcgt ttataggtac ccgttgagag aagattttca gcctgataca gattaaatct     4620

agacctaggc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     4680

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     4740

caggaaccgt aaaaa                                                      4755


<210>  33
<211>  7
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  33
atggtcc                                                                  7


<210>  34
<211>  7
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  34
acatcag                                                                  7


<210>  35
<211>  305
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  35

Met Thr Trp Leu Pro Leu Asn Pro Ile Leu Leu Lys Asp Arg Val Ser 
1               5                   10                  15      


Met Ile Phe Leu Gln Tyr Gly Gln Ile Asp Val Ile Asp Gly Ala Phe 
            20                  25                  30          


Val Leu Ile Asp Lys Thr Gly Ile Arg Thr His Ile Pro Val Gly Ser 
        35                  40                  45              


Val Ala Cys Ile Met Leu Glu Pro Gly Thr Arg Val Ser His Ala Ala 
    50                  55                  60                  


Val Arg Leu Ala Ala Gln Val Gly Thr Leu Leu Val Trp Val Gly Glu 
65                  70                  75                  80  


Ala Gly Val Arg Val Tyr Ala Ser Gly Gln Pro Gly Gly Ala Arg Ser 
                85                  90                  95      


Asp Lys Leu Leu Tyr Gln Ala Lys Leu Ala Leu Asp Glu Asp Leu Arg 
            100                 105                 110         


Leu Lys Val Val Arg Lys Met Phe Glu Leu Arg Phe Gly Glu Pro Ala 
        115                 120                 125             


Pro Ala Arg Arg Ser Val Glu Gln Leu Arg Gly Ile Glu Gly Ser Arg 
    130                 135                 140                 


Val Arg Ala Thr Tyr Ala Leu Leu Ala Lys Gln Tyr Gly Val Thr Trp 
145                 150                 155                 160 


Asn Gly Arg Arg Tyr Asp Pro Lys Asp Trp Glu Lys Gly Asp Thr Ile 
                165                 170                 175     


Asn Gln Cys Ile Ser Ala Ala Thr Ser Cys Leu Tyr Gly Val Thr Glu 
            180                 185                 190         


Ala Ala Ile Leu Ala Ala Gly Tyr Ala Pro Ala Ile Gly Phe Val His 
        195                 200                 205             


Thr Gly Lys Pro Leu Ser Phe Val Tyr Asp Ile Ala Asp Ile Ile Lys 
    210                 215                 220                 


Phe Asp Thr Val Val Pro Lys Ala Phe Glu Ile Ala Arg Arg Asn Pro 
225                 230                 235                 240 


Gly Glu Pro Asp Arg Glu Val Arg Leu Ala Cys Arg Asp Ile Phe Arg 
                245                 250                 255     


Ser Ser Lys Thr Leu Ala Lys Leu Ile Pro Leu Ile Glu Asp Val Leu 
            260                 265                 270         


Ala Ala Gly Glu Ile Gln Pro Pro Ala Pro Pro Glu Asp Ala Gln Pro 
        275                 280                 285             


Val Ala Ile Pro Leu Pro Val Ser Leu Gly Asp Ala Gly His Arg Ser 
    290                 295                 300                 


Ser 
305 


<210>  36
<211>  94
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  36

Met Ser Met Leu Val Val Val Thr Glu Asn Val Pro Pro Arg Leu Arg 
1               5                   10                  15      


Gly Arg Leu Ala Ile Trp Leu Leu Glu Val Arg Ala Gly Val Tyr Val 
            20                  25                  30          


Gly Asp Val Ser Ala Lys Ile Arg Glu Met Ile Trp Glu Gln Ile Ala 
        35                  40                  45              


Gly Leu Ala Gly Glu Gly Asn Val Val Met Ala Trp Ala Thr Asn Thr 
    50                  55                  60                  


Glu Thr Gly Phe Glu Phe Gln Thr Phe Gly Leu Asn Arg Arg Thr Pro 
65                  70                  75                  80  


Val Asp Leu Asp Gly Leu Arg Leu Val Ser Phe Leu Pro Val 
                85                  90                  


<210>  37
<211>  7
<212>  DNA
<213>  artificial sequence

<220>
<223>  Synthetic

<400>  37
ataaacc                                                                  7


