              LISTADO DE SECUENCIAS

<110> Instituto Nacional de Investigación y Tecnología Agraria y
      Alimentaria (INIA)
      The University Court of The University of Aberdeen

<120> Plásmido codificante para el receptor del factor de activación de células B (BAFF-R) y usos del mismo en el tratamiento y prevención de enfermedades inflamatorias en peces

<130> PCT3041.6

<150> ES P201931006
<151> 2019-11-19

<160> 18

<170> BiSSAP 1.3.6

<210> 1
<211> 60
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secuencia codificante del péptido señal de la IL-2 de la trucha
      arcoíris

<400> 1
atggaccgtc gttacaggat ttcctttttg acgctttttc tcaccggttg tctacaagga     60


<210> 2
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> Péptido señal IL-2 de la trucha arcoíris

<400> 2
Met Asp Arg Arg Tyr Arg Ile Ser Phe Leu Thr Leu Phe Leu Thr Gly 
1               5                   10                  15      
Cys Leu Gln Gly 
            20  

<210> 3
<211> 165
<212> DNA
<213> Artificial Sequence


<220> 
<223> Dominio extracelular de BAFF-R de la trucha arcoíris

<400> 3
atggcaaaga agacctgtga tccgggcaca agttgggaca aactgctcat cacctgtatc     60

cccttgacaa ggccaaagcc agccacagaa tccccacctg taaaatggag tagcgacgtt    120

cctccccagt acgacccggc agtgagtcca gctgtctgga tatca                    165


<210> 4
<211> 55
<212> PRT
<213> Artificial Sequence


<220> 
<223> Dominio extracelular de BAFF-R de la trucha arcoíris

<400> 4
Met Ala Lys Lys Thr Cys Asp Pro Gly Thr Ser Trp Asp Lys Leu Leu 
1               5                   10                  15      
Ile Thr Cys Ile Pro Leu Thr Arg Pro Lys Pro Ala Thr Glu Ser Pro 
            20                  25                  30          
Pro Val Lys Trp Ser Ser Asp Val Pro Pro Gln Tyr Asp Pro Ala Val 
        35                  40                  45              
Ser Pro Ala Val Trp Ile Ser 
    50                  55  

<210> 5
<211> 669
<212> DNA
<213> Artificial Sequence


<220> 
<223> Dominio Fc de la IgG1 de ratón

<400> 5
ggttgtaagc cttgcatatg tacagtccca gaagtatcat ctgtcttcat cttcccccca      60

aagcccaagg atgtgctcac cattactctg actcctaagg tcacgtgtgt tgtggtagac     120

atcagcaagg atgatcccga ggtccagttc agctggtttg tagatgatgt ggaggtgcac     180

acagctcaga cgcaaccccg ggaggagcag ttcaacagca ctttccgctc agtcagtgaa     240

cttcccatca tgcaccagga ctggctcaat ggcaaggagt tcaaatgcag ggtcaacagt     300

gcagctttcc ctgcccccat cgagaaaacc atctccaaaa ccaaaggcag accgaaggct     360

ccacaggtgt acaccattcc acctcccaag gagcagatgg ccaaggataa agtcagtctg     420

acctgcatga taacagactt cttccctgaa gacattactg tggagtggca gtggaatggg     480

cagccagcgg agaactacaa gaacactcag cccatcatgg acacagatgg ctcttacttc     540

gtctacagca agctcaatgt gcagaagagc aactgggagg caggaaatac tttcacctgc     600

tctgtgttac atgagggcct gcacaaccac catactgaga agagcctctc ccactctcct     660

ggtaaatga                                                             669


<210> 6
<211> 222
<212> PRT
<213> Artificial Sequence


<220> 
<223> Dominio Fc de la IgG1 de ratón

<400> 6
Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe 
1               5                   10                  15      
Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro 
            20                  25                  30          
Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val 
        35                  40                  45              
Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr 
    50                  55                  60                  
Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu 
65                  70                  75                  80  
Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 
                85                  90                  95      
Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            100                 105                 110         
Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro 
        115                 120                 125             
Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile 
    130                 135                 140                 
Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly 
145                 150                 155                 160 
Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp 
                165                 170                 175     
Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp 
            180                 185                 190         
Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His 
        195                 200                 205             
Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
    210                 215                 220         

<210> 7
<211> 225
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secuencia codificante de la construcción IL2-BAFF-R

<400> 7
atggaccgtc gttacaggat ttcctttttg acgctttttc tcaccggttg tctacaagga     60

atggcaaaga agacctgtga tccgggcaca agttgggaca aactgctcat cacctgtatc    120

cccttgacaa ggccaaagcc agccacagaa tccccacctg taaaatggag tagcgacgtt    180

cctccccagt acgacccggc agtgagtcca gctgtctgga tatca                    225


<210> 8
<211> 75
<212> PRT
<213> Artificial Sequence


<220> 
<223> Proteína de fusión IL2-BAFF-R

<400> 8
Met Asp Arg Arg Tyr Arg Ile Ser Phe Leu Thr Leu Phe Leu Thr Gly 
1               5                   10                  15      
Cys Leu Gln Gly Met Ala Lys Lys Thr Cys Asp Pro Gly Thr Ser Trp 
            20                  25                  30          
Asp Lys Leu Leu Ile Thr Cys Ile Pro Leu Thr Arg Pro Lys Pro Ala 
        35                  40                  45              
Thr Glu Ser Pro Pro Val Lys Trp Ser Ser Asp Val Pro Pro Gln Tyr 
    50                  55                  60                  
Asp Pro Ala Val Ser Pro Ala Val Trp Ile Ser 
65                  70                  75  

<210> 9
<211> 894
<212> DNA
<213> Artificial Sequence


<220> 
<223> Secuencia codificante de la construcción IL2-BAFF-R-IgG1

<400> 9
atggaccgtc gttacaggat ttcctttttg acgctttttc tcaccggttg tctacaagga      60

atggcaaaga agacctgtga tccgggcaca agttgggaca aactgctcat cacctgtatc     120

cccttgacaa ggccaaagcc agccacagaa tccccacctg taaaatggag tagcgacgtt     180

cctccccagt acgacccggc agtgagtcca gctgtctgga tatcaggttg taagccttgc     240

atatgtacag tcccagaagt atcatctgtc ttcatcttcc ccccaaagcc caaggatgtg     300

ctcaccatta ctctgactcc taaggtcacg tgtgttgtgg tagacatcag caaggatgat     360

cccgaggtcc agttcagctg gtttgtagat gatgtggagg tgcacacagc tcagacgcaa     420

ccccgggagg agcagttcaa cagcactttc cgctcagtca gtgaacttcc catcatgcac     480

caggactggc tcaatggcaa ggagttcaaa tgcagggtca acagtgcagc tttccctgcc     540

cccatcgaga aaaccatctc caaaaccaaa ggcagaccga aggctccaca ggtgtacacc     600

attccacctc ccaaggagca gatggccaag gataaagtca gtctgacctg catgataaca     660

gacttcttcc ctgaagacat tactgtggag tggcagtgga atgggcagcc agcggagaac     720

tacaagaaca ctcagcccat catggacaca gatggctctt acttcgtcta cagcaagctc     780

aatgtgcaga agagcaactg ggaggcagga aatactttca cctgctctgt gttacatgag     840

ggcctgcaca accaccatac tgagaagagc ctctcccact ctcctggtaa atga           894


<210> 10
<211> 297
<212> PRT
<213> Artificial Sequence


<220> 
<223> Proteína de fusión IL2-BAFF-R-IgG1

<400> 10
Met Asp Arg Arg Tyr Arg Ile Ser Phe Leu Thr Leu Phe Leu Thr Gly 
1               5                   10                  15      
Cys Leu Gln Gly Met Ala Lys Lys Thr Cys Asp Pro Gly Thr Ser Trp 
            20                  25                  30          
Asp Lys Leu Leu Ile Thr Cys Ile Pro Leu Thr Arg Pro Lys Pro Ala 
        35                  40                  45              
Thr Glu Ser Pro Pro Val Lys Trp Ser Ser Asp Val Pro Pro Gln Tyr 
    50                  55                  60                  
Asp Pro Ala Val Ser Pro Ala Val Trp Ile Ser Gly Cys Lys Pro Cys 
65                  70                  75                  80  
Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys 
                85                  90                  95      
Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val 
            100                 105                 110         
Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe 
        115                 120                 125             
Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu 
    130                 135                 140                 
Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His 
145                 150                 155                 160 
Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala 
                165                 170                 175     
Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg 
            180                 185                 190         
Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met 
        195                 200                 205             
Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro 
    210                 215                 220                 
Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn 
225                 230                 235                 240 
Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val 
                245                 250                 255     
Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr 
            260                 265                 270         
Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu 
        275                 280                 285             
Lys Ser Leu Ser His Ser Pro Gly Lys 
    290                 295         

<210> 11
<211> 6120
<212> DNA
<213> Artificial Sequence


<220> 
<223> Plásmido pBAFF-R

<400> 11
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttatgg accgtcgtta caggatttcc tttttgacgc tttttctcac      960

cggttgtcta caaggaatgg caaagaagac ctgtgatccg ggcacaagtt gggacaaact     1020

gctcatcacc tgtatcccct tgacaaggcc aaagccagcc acagaatccc cacctgtaaa     1080

atggagtagc gacgttcctc cccagtacga cccggcagtg agtccagctg tctggatatc     1140

aggttgtaag ccttgcatat gtacagtccc agaagtatca tctgtcttca tcttcccccc     1200

aaagcccaag gatgtgctca ccattactct gactcctaag gtcacgtgtg ttgtggtaga     1260

catcagcaag gatgatcccg aggtccagtt cagctggttt gtagatgatg tggaggtgca     1320

cacagctcag acgcaacccc gggaggagca gttcaacagc actttccgct cagtcagtga     1380

acttcccatc atgcaccagg actggctcaa tggcaaggag ttcaaatgca gggtcaacag     1440

tgcagctttc cctgccccca tcgagaaaac catctccaaa accaaaggca gaccgaaggc     1500

tccacaggtg tacaccattc cacctcccaa ggagcagatg gccaaggata aagtcagtct     1560

gacctgcatg ataacagact tcttccctga agacattact gtggagtggc agtggaatgg     1620

gcagccagcg gagaactaca agaacactca gcccatcatg gacacagatg gctcttactt     1680

cgtctacagc aagctcaatg tgcagaagag caactgggag gcaggaaata ctttcacctg     1740

ctctgtgtta catgagggcc tgcacaacca ccatactgag aagagcctct cccactctcc     1800

tggtaaatga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc     1860

ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg     1920

tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag     1980

gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga     2040

caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag     2100

ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt     2160

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc     2220

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg     2280

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta     2340

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt     2400

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat     2460

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa     2520

tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg     2580

tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag     2640

tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg     2700

catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact     2760

ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag     2820

gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc     2880

ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag     2940

acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc     3000

gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat     3060

gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg     3120

tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg     3180

ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta     3240

ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta     3300

tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc     3360

gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc     3420

gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg     3480

ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg     3540

ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt     3600

gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc     3660

ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc     3720

atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga     3780

ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg     3840

aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg     3900

atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca     3960

aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt     4020

gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct     4080

agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa     4140

ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga     4200

gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt     4260

gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct     4320

cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat     4380

cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga     4440

acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt     4500

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt     4560

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     4620

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     4680

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     4740

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     4800

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     4860

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     4920

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta     4980

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg     5040

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt     5100

tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg     5160

tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta     5220

aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg     5280

aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg     5340

tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc     5400

gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg     5460

agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg     5520

aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag     5580

gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat     5640

caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc     5700

cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc     5760

ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa     5820

ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac     5880

gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt     5940

cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc     6000

gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa     6060

caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca     6120


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> cebador T7

<400> 12
taatacgact cactataggg                                                20


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Cebador directo del gen EF-1 alpha de la trucha arcoíris

<400> 13
gatccagaag gaggtcacca                                                20


<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Cebador reverso del gen EF-1 alpha de la trucha arcoíris

<400> 14
ttacgttcga ccttccatcc                                                20


<210> 15
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Cebador directo del gen BAFF-R de la trucha arcoíris

<400> 15
gacaaactgc tcatcacctg tatc                                           24


<210> 16
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Cebador reverso del gen BAFF-R de la trucha arcoíris

<400> 16
atatccagac agctggactc actg                                           24


<210> 17
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> Cebador directo del gen ARNr18S de Tetracapsuloides bryosalmonae

<400> 17
ggacactgca tgtgctgcat agt                                            23


<210> 18
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> Cebador reverso del gen ARNr18S de Tetracapsuloides bryosalmonae

<400> 18
ccatgctaga atgtccaggc act                                            23


