                         SEQUENCE LISTING

<110>  Medigene Immunotherapies GmbH
 
<120>  PRAME TCR RECEPTORS AND USES THEREOF

<130>  MED16815PCT

<150>	EP19209757.4
<151>	2019-11-18

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  PRAME epitope, tumor associated antigene

<400>  1

Ser Leu Leu Gln His Leu Ile Gly Leu 
1               5                   


<210>  2
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  CDR1 alpha, human modified

<400>  2

Ser Ile Phe Asn Thr 
1               5   


<210>  3
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  CDR1 beta, human modified

<400>  3

Ser Gly Asp Leu Ser 
1               5   


<210>  4
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  CDR2 alpha, human modified

<400>  4

Leu Tyr Lys Ala Gly Glu Leu 
1               5           


<210>  5
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  CDR2 beta, human modified

<400>  5

Tyr Tyr Asn Gly Glu Glu 
1               5       


<210>  6
<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  CDR3 alpha, human modified

<400>  6

Cys Ala Gly Leu Ala Asp Tyr Gly Gly Ser Gln Gly Asn Leu Ile Phe 
1               5                   10                  15      


<210>  7
<211>  15
<212>  PRT
<213>  artificial

<220>
<223>  CDR3 beta, human modified

<400>  7

Cys Ala Ser Ser Val Trp Ala Ser Gly Gly Tyr Glu Gln Tyr Phe 
1               5                   10                  15  


<210>  8
<211>  108
<212>  PRT
<213>  artificial

<220>
<223>  TCR alpha variable region, human modified

<400>  8

Met Leu Leu Glu His Leu Leu Ile Ile Leu Trp Met Gln Leu Thr Trp 
1               5                   10                  15      


Val Ser Gly Gln Gln Leu Asn Gln Ser Pro Gln Ser Met Phe Ile Gln 
            20                  25                  30          


Glu Gly Glu Asp Val Ser Met Asn Cys Thr Ser Ser Ser Ile Phe Asn 
        35                  40                  45              


Thr Trp Leu Trp Tyr Lys Gln Asp Pro Gly Glu Gly Pro Val Leu Leu 
    50                  55                  60                  


Ile Ala Leu Tyr Lys Ala Gly Glu Leu Thr Ser Asn Gly Arg Leu Thr 
65                  70                  75                  80  


Ala Gln Phe Gly Ile Thr Arg Lys Asp Ser Phe Leu Asn Ile Ser Ala 
                85                  90                  95      


Ser Ile Pro Ser Asp Val Gly Ile Tyr Phe Cys Ala 
            100                 105             


<210>  9
<211>  114
<212>  PRT
<213>  artificial

<220>
<223>  TCR beta variable region, human modified

<400>  9

Met Gly Phe Arg Leu Leu Cys Cys Val Ala Phe Cys Leu Leu Gly Ala 
1               5                   10                  15      


Gly Pro Val Asp Ser Gly Val Thr Gln Thr Pro Lys His Leu Ile Thr 
            20                  25                  30          


Ala Thr Gly Gln Arg Val Thr Leu Arg Cys Ser Pro Arg Ser Gly Asp 
        35                  40                  45              


Leu Ser Val Tyr Trp Tyr Gln Gln Ser Leu Asp Gln Gly Leu Gln Phe 
    50                  55                  60                  


Leu Ile Gln Tyr Tyr Asn Gly Glu Glu Arg Ala Lys Gly Asn Ile Leu 
65                  70                  75                  80  


Glu Arg Phe Ser Ala Gln Gln Phe Pro Asp Leu His Ser Glu Leu Asn 
                85                  90                  95      


Leu Ser Ser Leu Glu Leu Gly Asp Ser Ala Leu Tyr Phe Cys Ala Ser 
            100                 105                 110         


Ser Val 
        


<210>  10
<211>  273
<212>  PRT
<213>  artificial

<220>
<223>  TCR alpha chain (mmC), human modified

<400>  10

Met Leu Leu Glu His Leu Leu Ile Ile Leu Trp Met Gln Leu Thr Trp 
1               5                   10                  15      


Val Ser Gly Gln Gln Leu Asn Gln Ser Pro Gln Ser Met Phe Ile Gln 
            20                  25                  30          


Glu Gly Glu Asp Val Ser Met Asn Cys Thr Ser Ser Ser Ile Phe Asn 
        35                  40                  45              


Thr Trp Leu Trp Tyr Lys Gln Asp Pro Gly Glu Gly Pro Val Leu Leu 
    50                  55                  60                  


Ile Ala Leu Tyr Lys Ala Gly Glu Leu Thr Ser Asn Gly Arg Leu Thr 
65                  70                  75                  80  


Ala Gln Phe Gly Ile Thr Arg Lys Asp Ser Phe Leu Asn Ile Ser Ala 
                85                  90                  95      


Ser Ile Pro Ser Asp Val Gly Ile Tyr Phe Cys Ala Gly Leu Ala Asp 
            100                 105                 110         


Tyr Gly Gly Ser Gln Gly Asn Leu Ile Phe Gly Lys Gly Thr Lys Leu 
        115                 120                 125             


Ser Val Lys Pro Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu 
    130                 135                 140                 


Arg Asp Ser Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe 
145                 150                 155                 160 


Asp Ser Gln Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile 
                165                 170                 175     


Thr Asp Lys Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn 
            180                 185                 190         


Ser Ala Val Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala 
        195                 200                 205             


Phe Asn Asn Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Ser Asp 
    210                 215                 220                 


Val Pro Cys Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr 
225                 230                 235                 240 


Asn Leu Asn Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu 
                245                 250                 255     


Leu Lys Val Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser 
            260                 265                 270         


Ser 
    


<210>  11
<211>  312
<212>  PRT
<213>  artificial

<220>
<223>  TCR beta chain mmC, human modified

<400>  11

Met Gly Phe Arg Leu Leu Cys Cys Val Ala Phe Cys Leu Leu Gly Ala 
1               5                   10                  15      


Gly Pro Val Asp Ser Gly Val Thr Gln Thr Pro Lys His Leu Ile Thr 
            20                  25                  30          


Ala Thr Gly Gln Arg Val Thr Leu Arg Cys Ser Pro Arg Ser Gly Asp 
        35                  40                  45              


Leu Ser Val Tyr Trp Tyr Gln Gln Ser Leu Asp Gln Gly Leu Gln Phe 
    50                  55                  60                  


Leu Ile Gln Tyr Tyr Asn Gly Glu Glu Arg Ala Lys Gly Asn Ile Leu 
65                  70                  75                  80  


Glu Arg Phe Ser Ala Gln Gln Phe Pro Asp Leu His Ser Glu Leu Asn 
                85                  90                  95      


Leu Ser Ser Leu Glu Leu Gly Asp Ser Ala Leu Tyr Phe Cys Ala Ser 
            100                 105                 110         


Ser Val Trp Ala Ser Gly Gly Tyr Glu Gln Tyr Phe Gly Pro Gly Thr 
        115                 120                 125             


Arg Leu Thr Val Thr Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val 
    130                 135                 140                 


Ala Val Phe Glu Pro Ser Lys Ala Glu Ile Ala His Thr Gln Lys Ala 
145                 150                 155                 160 


Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu 
                165                 170                 175     


Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp 
            180                 185                 190         


Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys 
        195                 200                 205             


Leu Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg 
    210                 215                 220                 


Asn His Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp 
225                 230                 235                 240 


Glu Trp Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala 
                245                 250                 255     


Glu Ala Trp Gly Arg Ala Asp Cys Gly Ile Thr Ser Arg Ser Tyr His 
            260                 265                 270         


Gln Gly Val Leu Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys 
        275                 280                 285             


Ala Thr Leu Tyr Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met 
    290                 295                 300                 


Val Lys Arg Lys Asp Ser Arg Gly 
305                 310         


<210>  12
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  PRAME epitope, tumor associated antigen

<400>  12
agcctgctgc agcatctgat tggcctg                                           27


<210>  13
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  CDR1 alpha, human modified

<400>  13
agcatattta acacc                                                        15


<210>  14
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  CDR1 beta, human modified

<400>  14
tctggagacc tctct                                                        15


<210>  15
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  CDR2 alpha, human modified

<400>  15
ttatataagg ctggtgaatt g                                                 21


<210>  16
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  CDR2 beta, human modified

<400>  16
tattataatg gagaagag                                                     18


<210>  17
<211>  48
<212>  DNA
<213>  artificial

<220>
<223>  CDR3 alpha, human modified

<400>  17
tgtgctgggc ttgctgatta tggaggaagc caaggaaatc tcatcttt                    48


<210>  18
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  CDR3 beta, human modified

<400>  18
tgtgccagca gcgtatgggc ctccggcggc tacgagcagt acttc                       45


<210>  19
<211>  324
<212>  DNA
<213>  artificial

<220>
<223>  TCR alpha variable region, human modified

<400>  19
atgctgctgg aacatctgct gatcatcctg tggatgcagc tgacctgggt ttccggccag       60

cagctgaatc agagccctca gagcatgttc atccaagaag gcgaggacgt ttccatgaat      120

tgcaccagca gcagcatctt caacacctgg ctgtggtaca agcaggaccc tggcgaagga      180

ccagtgctgc tgatcgcctt gtacaaagcc ggcgagctga ccagcaacgg cagactgaca      240

gcccagttcg gcattacccg gaaggacagc ttcctgaaca tctccgccag cattccctcc      300

gacgtgggca tctatttttg tgcc                                             324


<210>  20
<211>  342
<212>  DNA
<213>  artificial

<220>
<223>  TCR beta variable region, human modified

<400>  20
atgggcttca gactgctgtg ctgcgtggcc ttttgtctgc ttggagccgg acctgtggat       60

agcggcgtta cccagacacc taagcacctg atcacagcca caggccagcg cgtgaccctg      120

agatgttctc ctagaagcgg cgacctgagc gtgtactggt atcagcagtc tctggaccag      180

ggcctgcagt tcctgatcca gtactacaac ggcgaggaaa gagccaaggg caacatcctg      240

gaacggttca gcgcccagca gttcccagat ctgcacagcg agctgaacct gagcagcctg      300

gaactgggag atagcgccct gtacttctgt gcctctagcg tg                         342


<210>  21
<211>  819
<212>  DNA
<213>  artificial

<220>
<223>  TCR alpha chain (mmC), human modified

<400>  21
atgctgctgg aacatctgct gatcatcctg tggatgcagc tgacctgggt ttccggccag       60

cagctgaatc agagccctca gagcatgttc atccaagaag gcgaggacgt ttccatgaat      120

tgcaccagca gcagcatctt caacacctgg ctgtggtaca agcaggaccc tggcgaagga      180

ccagtgctgc tgatcgcctt gtacaaagcc ggcgagctga ccagcaacgg cagactgaca      240

gcccagttcg gcattacccg gaaggacagc ttcctgaaca tctccgccag cattccctcc      300

gacgtgggca tctatttttg tgccggcctg gccgattacg gcggctctca gggaaatctg      360

atcttcggca agggcaccaa gctgagcgtg aagcccaaca ttcagaaccc cgatcctgcc      420

gtgtaccagc tgagagacag caagagcagc gacaagagcg tgtgcctgtt caccgacttc      480

gacagccaga ccaacgtgtc ccagagcaag gacagcgacg tgtacatcac cgacaagacc      540

gtgctggaca tgcggagcat ggacttcaag agcaacagcg ccgtggcctg gtccaacaag      600

agcgatttcg cctgcgccaa cgccttcaac aacagcatta tccccgagga cacattcttc      660

cccagctccg atgtgccctg cgacgtgaag ctggtggaaa agagcttcga gacagacacc      720

aacctgaact tccagaacct gtccgtgatc ggcttcagaa tcctgctgct gaaggtggcc      780

ggcttcaacc tgctgatgac actgagactg tggtccagc                             819


<210>  22
<211>  936
<212>  DNA
<213>  artificial

<220>
<223>  TCR beta chain (mmC), human modified

<400>  22
atgggcttca gactgctgtg ctgcgtggcc ttttgtctgc ttggagccgg acctgtggat       60

agcggcgtta cccagacacc taagcacctg atcacagcca caggccagcg cgtgaccctg      120

agatgttctc ctagaagcgg cgacctgagc gtgtactggt atcagcagtc tctggaccag      180

ggcctgcagt tcctgatcca gtactacaac ggcgaggaaa gagccaaggg caacatcctg      240

gaacggttca gcgcccagca gttcccagat ctgcacagcg agctgaacct gagcagcctg      300

gaactgggag atagcgccct gtacttctgt gcctctagcg tgtgggcctc tggcggctac      360

gagcagtatt ttggccctgg caccagactg accgtgaccg aggatctgaa gaacgtgttc      420

ccacctgagg tggccgtgtt cgagccttct aaggccgaga ttgcccacac acagaaagcc      480

acactcgtgt gtctggccac cggcttctat cccgatcacg tggaactgtc ttggtgggtc      540

aacggcaaag aggtgcacag cggcgtcagc acagatcccc agcctctgaa agaacagccc      600

gctctgaacg acagccggta ctgtctgagc agcagactga gagtgtccgc caccttctgg      660

cagaacccca gaaaccactt cagatgccag gtgcagttct acggcctgag cgagaacgat      720

gagtggaccc aggacagagc taagcccgtg acacagatcg tgtctgccga agcttggggc      780

agagccgatt gtggcatcac cagcagatct taccaccagg gcgtgctgag cgccaccatc      840

ctgtatgaga tcctgctggg caaagccact ctgtacgccg tgctggtgtc tgccctggtg      900

ctgatggcca tggtcaagcg gaaggatagc agaggc                                936


<210>  23
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  3825 CDR3 alpha, derived from mus musculus

<400>  23

Cys Ala Val Glu Pro Gly Gly Ser Tyr Ile Pro Thr Phe 
1               5                   10              


<210>  24
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  3825 CDR3 beta, derived from mus musculus

<400>  24

Cys Ala Ser Ser Pro Gly Leu Ser Tyr Glu Gln Tyr Phe 
1               5                   10              


<210>  25
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  Irrelevant paptide, human

<400>  25

Gly Leu Ser Asn Thr His Val Leu 
1               5               


<210>  26
<211>  140
<212>  PRT
<213>  artificial

<220>
<223>  TCR alpha constant, derived from human

<400>  26

Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
1               5                   10                  15      


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
            20                  25                  30          


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
        35                  40                  45              


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
    50                  55                  60                  


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
65                  70                  75                  80  


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp Val 
                85                  90                  95      


Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
            100                 105                 110         


Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
        115                 120                 125             


Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140 


<210>  27
<211>  177
<212>  PRT
<213>  artificial

<220>
<223>  TCR beta-01 constant, derived from human

<400>  27

Glu Asp Leu Asn Lys Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys Gly Phe Thr Ser Val Ser Tyr Gln Gln Gly Val Leu Ser 
    130                 135                 140                 


Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 


Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     


Phe 
    


<210>  28
<211>  179
<212>  PRT
<213>  artificial

<220>
<223>  TCR beta-02 constant, derived from human

<400>  28

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser 
    130                 135                 140                 


Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 


Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     


Ser Arg Gly 
            


<210>  29
<211>  140
<212>  PRT
<213>  artificial

<220>
<223>  TCR alpha constant, derived from mus musculus

<400>  29

Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
1               5                   10                  15      


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
            20                  25                  30          


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
        35                  40                  45              


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
    50                  55                  60                  


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
65                  70                  75                  80  


Ile Pro Glu Asp Thr Phe Phe Pro Ser Ser Asp Val Pro Cys Asp Val 
                85                  90                  95      


Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
            100                 105                 110         


Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
        115                 120                 125             


Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140 


<210>  30
<211>  179
<212>  PRT
<213>  artificial

<220>
<223>  TCR beta constant, derived from mus musculus

<400>  30

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Lys Ala Glu Ile Ala His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys Gly Ile Thr Ser Arg Ser Tyr His Gln Gly Val Leu Ser 
    130                 135                 140                 


Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 


Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     


Ser Arg Gly 
            


<210>  31
<211>  136
<212>  PRT
<213>  artificial

<220>
<223>  TCR alpha murC constant, murinized

<400>  31

Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser 
1               5                   10                  15      


Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn 
            20                  25                  30          


Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Thr Val 
        35                  40                  45              


Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp 
    50                  55                  60                  


Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn 
65                  70                  75                  80  


Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu 
                85                  90                  95      


Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val 
            100                 105                 110         


Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu 
        115                 120                 125             


Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135     


<210>  32
<211>  173
<212>  PRT
<213>  artificial

<220>
<223>  TCR beta murC constant, murinized

<400>  32

Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Thr Leu Phe Glu Pro 
1               5                   10                  15      


Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Ala Tyr Lys 
    50                  55                  60                  


Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala 
65                  70                  75                  80  


Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe 
                85                  90                  95      


His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser Pro Lys Pro 
            100                 105                 110         


Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly 
        115                 120                 125             


Ile Thr Ser Ala Ser Tyr His Gln Gly Val Leu Ser Ala Thr Ile Leu 
    130                 135                 140                 


Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser 
145                 150                 155                 160 


Gly Leu Val Leu Met Ala Met Val Lys Lys Lys Asn Ser 
                165                 170             


<210>  33
<211>  509
<212>  PRT
<213>  artificial

<220>
<223>  PRAME full-length, derived from human

<400>  33

Met Glu Arg Arg Arg Leu Trp Gly Ser Ile Gln Ser Arg Tyr Ile Ser 
1               5                   10                  15      


Met Ser Val Trp Thr Ser Pro Arg Arg Leu Val Glu Leu Ala Gly Gln 
            20                  25                  30          


Ser Leu Leu Lys Asp Glu Ala Leu Ala Ile Ala Ala Leu Glu Leu Leu 
        35                  40                  45              


Pro Arg Glu Leu Phe Pro Pro Leu Phe Met Ala Ala Phe Asp Gly Arg 
    50                  55                  60                  


His Ser Gln Thr Leu Lys Ala Met Val Gln Ala Trp Pro Phe Thr Cys 
65                  70                  75                  80  


Leu Pro Leu Gly Val Leu Met Lys Gly Gln His Leu His Leu Glu Thr 
                85                  90                  95      


Phe Lys Ala Val Leu Asp Gly Leu Asp Val Leu Leu Ala Gln Glu Val 
            100                 105                 110         


Arg Pro Arg Arg Trp Lys Leu Gln Val Leu Asp Leu Arg Lys Asn Ser 
        115                 120                 125             


His Gln Asp Phe Trp Thr Val Trp Ser Gly Asn Arg Ala Ser Leu Tyr 
    130                 135                 140                 


Ser Phe Pro Glu Pro Glu Ala Ala Gln Pro Met Thr Lys Lys Arg Lys 
145                 150                 155                 160 


Val Asp Gly Leu Ser Thr Glu Ala Glu Gln Pro Phe Ile Pro Val Glu 
                165                 170                 175     


Val Leu Val Asp Leu Phe Leu Lys Glu Gly Ala Cys Asp Glu Leu Phe 
            180                 185                 190         


Ser Tyr Leu Ile Glu Lys Val Lys Arg Lys Lys Asn Val Leu Arg Leu 
        195                 200                 205             


Cys Cys Lys Lys Leu Lys Ile Phe Ala Met Pro Met Gln Asp Ile Lys 
    210                 215                 220                 


Met Ile Leu Lys Met Val Gln Leu Asp Ser Ile Glu Asp Leu Glu Val 
225                 230                 235                 240 


Thr Cys Thr Trp Lys Leu Pro Thr Leu Ala Lys Phe Ser Pro Tyr Leu 
                245                 250                 255     


Gly Gln Met Ile Asn Leu Arg Arg Leu Leu Leu Ser His Ile His Ala 
            260                 265                 270         


Ser Ser Tyr Ile Ser Pro Glu Lys Glu Glu Gln Tyr Ile Ala Gln Phe 
        275                 280                 285             


Thr Ser Gln Phe Leu Ser Leu Gln Cys Leu Gln Ala Leu Tyr Val Asp 
    290                 295                 300                 


Ser Leu Phe Phe Leu Arg Gly Arg Leu Asp Gln Leu Leu Arg His Val 
305                 310                 315                 320 


Met Asn Pro Leu Glu Thr Leu Ser Ile Thr Asn Cys Arg Leu Ser Glu 
                325                 330                 335     


Gly Asp Val Met His Leu Ser Gln Ser Pro Ser Val Ser Gln Leu Ser 
            340                 345                 350         


Val Leu Ser Leu Ser Gly Val Met Leu Thr Asp Val Ser Pro Glu Pro 
        355                 360                 365             


Leu Gln Ala Leu Leu Glu Arg Ala Ser Ala Thr Leu Gln Asp Leu Val 
    370                 375                 380                 


Phe Asp Glu Cys Gly Ile Thr Asp Asp Gln Leu Leu Ala Leu Leu Pro 
385                 390                 395                 400 


Ser Leu Ser His Cys Ser Gln Leu Thr Thr Leu Ser Phe Tyr Gly Asn 
                405                 410                 415     


Ser Ile Ser Ile Ser Ala Leu Gln Ser Leu Leu Gln His Leu Ile Gly 
            420                 425                 430         


Leu Ser Asn Leu Thr His Val Leu Tyr Pro Val Pro Leu Glu Ser Tyr 
        435                 440                 445             


Glu Asp Ile His Gly Thr Leu His Leu Glu Arg Leu Ala Tyr Leu His 
    450                 455                 460                 


Ala Arg Leu Arg Glu Leu Leu Cys Glu Leu Gly Arg Pro Ser Met Val 
465                 470                 475                 480 


Trp Leu Ser Ala Asn Pro Cys Pro His Cys Gly Asp Arg Thr Phe Tyr 
                485                 490                 495     


Asp Pro Glu Pro Ile Leu Cys Pro Cys Phe Met Pro Asn 
            500                 505                 


