               SEQUENCE LISTING

<110> Justus-Liebig-Universität Gießen

<120> A new antibiotic selectively kills Gram-negative pathogens

<130> TM1077

<150> EP19210353.9
<151> 2019-11-20

<160> 37

<170> BiSSAP 1.3.6

<210> 1
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 1
tttgacgttg gagtccacgt gttatggacg tggcaaacgc ggttcttgac                50


<210> 2
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 2
ttgaaatatc aggatagcat tgcgctcgct caccccggtc acatagttcg                50


<210> 3
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 3
atgctatcct gatatttcaa atgcaagtaa aatgtttcat cataataacc                50


<210> 4
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 4
ttcttgacga gttcttctga gatgggttga tatccactga tataaatctc                50


<210> 5
<211> 55
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 5
tcgagctcta aggaggttat aaaaaatgaa catcaaaaag tttgcaaaac aagca          55


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 6
acgtggactc caacgtcaaa                                                20


<210> 7
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 7
actcttcctt tttcaatatt attgaagcat                                     30


<210> 8
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 8
tgcatttttt ataacctcct tagagctcga attcc                                35


<210> 9
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 9
tcagaagaac tcgtcaagaa ggcga                                          25


<210> 10
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 10
tcaataatat tgaaaaagga agagtatgat tgaacaagat ggattgcacg                50


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 11
atctccatca aagcgctacc                                                20


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 12
ccgcgctgca actcgaaatc                                                20


<210> 13
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 13
ggtcgacgga tccccggaat agcggaaatg gcttacgaac                           40


<210> 14
<211> 48
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 14
ctctaaggag gttataaaaa gcggccgcat cccttaacgt gagttttc                  48


<210> 15
<211> 43
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 15
aagcagctcc agcctacatc agaagaactc gtcaagaagg cga                       43


<210> 16
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 16
tttttataac ctccttagag ctcgaattcc                                     30


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 17
attccgggga tccgtcgacc                                                20


<210> 18
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 18
tgtaggctgg agctgctt                                                  18


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 19
tcccttaacg tgagttttcg                                                20


<210> 20
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 20
ttttataacc tccttagagc tcgaa                                          25


<210> 21
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 21
gctctaagga ggttataaaa atgcataata ccttaaatga aaccgttaaa                50


<210> 22
<211> 46
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 22
aatagcattc atttatggct ctccttttaa atttcctgga agcttt                    46


<210> 23
<211> 46
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 23
aaagcttcca ggaaatttaa aaggagagcc ataaatgaat gctatt                    46


<210> 24
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer

<400> 24
cgaaaactca cgttaaggga ttacgccgcg atggtttgtt ttatt                     45


<210> 25
<211> 1290
<212> DNA
<213> Photorhabdus sp.


<220> 
<223> Photorhabdus_namnaonensis_darE_codon-optimized

<400> 25
atggacacca tcatcccgat caaatacctg aacgctgacg aatcttctat cctgaaaaaa      60

tctccgaaaa tcaactaccg tcagctggct tgccgtatca tcggtgaaat cccggctgaa     120

aaaatcctgg acgacgacga actggctctg tacaacgaag aaatcggtat ccacttctct     180

ccggaaatca tcaacgctaa caaactggtt gttgttgtta aagctacccg tctgtgcaac     240

ctgcgttgca cctactgcca ctcttgggct gaaggtaaag gtaacaccct gaccttcttc     300

aacctgatgc gttctatcca ccgtttcctg tctatcccga acatcaaacg tttcgaattc     360

gtttggcacg gtggtgaagt taccctgctg tctgttaact acttcaaaaa actgatctgg     420

ctgcaggaac agttcaaaaa accggaccag gttatcacca actctgttca gaccaacgct     480

gttaacatcc cggaagactg gctggttttc ctgaaaggta tcggtatggg tgttggtatc     540

tctgttgacg gtatcccgga aatccacgac tctcgtcgtc tggactaccg tggtcgtccg     600

acctctcaca aagttgctgc tggtatgaaa aaactgcgtt cttacggtat cccgtacggt     660

gctctggttg ttgttgaccg tgacgtttac gaatctaaca tcgaaaaaat gctgtcttac     720

ttctacgaaa tcggtctgac cgacatcgaa ttcctgaaca tcgttccgga caaccgttgc     780

cagccgggtg acgacccggg tggttcttac atcacctacc acaactacat caacttcctg     840

tctaacgttt tccgtgtttg gtggaacgac taccaggaca aaatcaacat ccgtctgttc     900

cacggtttca tcgactctat caaatcttct cagaaaaaaa tctctgactg ctactgggct     960

ggtaactgct ctcaggaaat catcaccctg gaaccgaacg gtaccgtttc tgcttgcgac    1020

aaatacgttg gtgctgaagg taacaactac ggttctatca tcgacaacga cctgggtcac    1080

ctgctgatca aatctaacac caacaaaaac cacctgaaag aagaaatcga atcttacgaa    1140

aaaatgcacc agtgcaaatg gttccacctg tgcaacggtg gttgcccgca cgaccgtgtt    1200

accaaccgta aacacaaccc gaactacaac gacttctgct gcggtaccgg tggtctgctg    1260

gaaatcatca aacagaccat cgctgcttaa                                     1290


<210> 26
<211> 177
<212> DNA
<213> Photorhabdus sp.


<220> 
<223> Photorhabdus_namnaonensis_darA_codon-optimized

<400> 26
atgcacaaca cctctatcat caactgcacc acccaggaag ctctgaactc tctggctgct     60

tctttcaaag acaccgaact gtctatcacc gaacgtgctc tggacgaact gaacaacaaa    120

ccgaaaatcc cggaaatcac cgcttggaac tggtctaaat ctttccagga aatctaa       177


<210> 27
<211> 177
<212> DNA
<213> Pseudoalteromonas luteoviolacea


<220> 
<223> Pseudoalteromonas_luteoviolacea_darA_codon-optimized

<400> 27
atgatcgttg aagctccgaa agaaaaagtt tctatctctg aaaaactgga cgctctgaaa     60

tcttctttct ctaaccagac cctgaacatc gctaacgttg accaggctcg tgttgactct    120

atctctgttg ctccgccgat caccgcttgg aactggtcta aatctttcga aaaataa       177


<210> 28
<211> 1287
<212> DNA
<213> Pseudoalteromonas luteoviolacea


<220> 
<223> Pseudoalteromonas_luteoviolacea_darE_codon-optimized

<400> 28
atgctgggtg acatctctgt taaagttatc gaagaaccgg ttaacccggt taaccagctg      60

gaactgaccg actaccagta ctctcgtctg gcttctcagc tgatcggtga aatcccggac     120

tctgacatgc tggacgaaga agaactggct ctgttccgtc gtgaaaaaga cgcttacttc     180

gaagctaaac cgctgaacgc ttctaaagtt gttgttgttc tgaaagctac ccgtctgtgc     240

aacctgcgtt gcacctactg ccactcttgg gctgaaggtc cgaaccagac cgttaaattc     300

gaaaacctga tctctatcgt taaacgtatc ctggctatcc cgaacgtttc tcgtgttgaa     360

ttcgtttggc acggtggtga agttaccctg ctgcgtccgg ctttcttcaa aaaactgatc     420

tggctgcagg aacagttcaa acgtaaagac cacttcatca ccaacaccat gcagtctaac     480

gctgttaaca tctctaaaga atggctgacc ttcctgcagg gtatcggtat ggctgttggt     540

atctctttcg acggtgttcc ggaaatcaac gacacccgtc gtctggacgt tcgtggtcgt     600

ccgacctctc tgaaagttgc tgaaggtatc aaacgtctgc aggaatacgg tatcccgtac     660

ggtgctctga tcgttgttga ccgtgacgtt tacaacgttt ctccggaacg tctgctgtct     720

tacctggctt ctatcgaact gaacgacatc gaattcctga acatcgttcc ggacaaccgt     780

gctcagccgg gtgacgacat cggtgacgct tacatctctt acaaagaata catcgaattc     840

ctgtctcgtg tttacaccgt ttggcacgct cagtaccgtg gtatcatcca gatccgtatg     900

ttcgaaaact tcatggacgt tctggctgac cgttctaaac agctgtctgc ttgctactgg     960

gctggtaact gctctcagga aatcatcacc atcgaaccga acggtgacgt ttctccgtgc    1020

gacaaatacg ttggtgacat cggttctatc tacggttctc tgctggactc tgacctggct    1080

accctgctgg ctgactctaa acacaaccag acctctgttc tggaagaagt tgaatctcac    1140

tctcgtatgc acgaatgcaa atggttctct atctgcaacg gtggttgccc gcacgaccgt    1200

gttatcaacg ctcgtcacgt tgaagactac gacgacaaat gctgcggtac cggtaaactg    1260

ctgaaagtta tcgaagaatc tatctaa                                        1287


<210> 29
<211> 1518
<212> DNA
<213> Pseudoalteromonas luteoviolacea


<220> 
<223> Pseudoalteromonas_luteoviolacea_Darobactin-halogenase_codon-optim
      ized

<400> 29
atggaatcta accaccaggt tgttatcatc ggtgctggtc cggctggtat gcagctgtct      60

tacttcctga acaaagcttc tgtttctcac gttgttctgg aacgtgctga catcccgggt     120

tctttcttcg ctacctaccc ggttcaccgt aaactgatct ctatcaacaa agttcacacc     180

ggtttcgacg acaaaaccaa aaacctgcgt tgggactgga actctctgct gaacgacgac     240

gacgacttct ctttcaaaga cttcgaccag tcttactacc cgtcttctga caaactggtt     300

gaatacatgg ctcagttcgc tacccgttac gaactgccga tccagttcaa cacccacgtt     360

gactctatca ccaaaaccaa ctctcagttc gttatctctt gccgtaacaa caaaacctac     420

aacgctaaca tcctggttga cgctaccggt atcggtgaac tgaacgttcc gaacatcgac     480

ggtgttgaac acgttatccc gtacaccgaa atgtctaacg acgtttctca gttcaaaaac     540

aaacgtatcc tgatcctggg taaaggtaac tctgctttcg aaaccgctga ctctctgatg     600

gaagtttctg ctaacaccca cgttatctct ccgaaagact tcaacttcgc ttgggtttct     660

cactacccgg gtcacctgcg ttctgttaac cagggtttcc tggacacctt cttcctgaaa     720

cagcagaacg ctatcctgaa cggtaccatc gcttctatca aaccgaccga acagggtcag     780

tacctggttg acatgatctt ctctgaagac ggtgaacgtg aatctaacac ctacgaccac     840

atcgttaact gctctggttt caaatgcaac ttcgaccact actctgctga cctgaaaccg     900

gacctgtgcc tgatggacaa attcccgaaa ctgaaccacc agtggcagtc taccaacatc     960

gacaacctgt tcttctgcgg tgctaacatg cagtgcaacg actacaaaga ctcttctacc    1020

ccgttcgttc acggtatccg tcacaacgct cagaccctgg ctgaaatcct ggttaacaaa    1080

ctgcagaacc gtacctggca ggctaaacag accatcaccg ctgaaaccga cctgtacgac    1140

gctatctctg aacagatccg tcgtgctacc accatctggt tcctgttcgg taacgtttac    1200

gacgtttacg ctctggacgc tcagaacaac ttccagttct acgaatctat cccgaaactg    1260

gttttcgaac aggaagaact gttcaaaaac ttctctggtt acaccctgga attctcttac    1320

aaactgccga aagacgaaaa cgaaaaaccg aaattctcta cccacggttt cctgcacccg    1380

atcatcatga aataccaggg tggtgaatgc atcgacgaaa tccacatgct ggaagacatc    1440

tactctgaat gggaagacct ggacaaactg gacaacggta tcaaagaatt catcctgacc    1500

tctcagttcg aaatctaa                                                  1518


<210> 30
<211> 1065
<212> DNA
<213> Pseudoalteromonas luteoviolacea


<220> 
<223> Pseudoalteromonas_luteoviolacea_protein_wo_homology_codon-optimiz
      ed

<400> 30
atgaacaccc tgaccaacga actgccgaaa tgcctggaat tcaactctga caacttcgct      60

gacatcttct ctcagtgcaa aatcgacctg ctggttgaca tcttcaaacg tgaaatcctg     120

tctcacaaag aactgtctga actgttcgac aaactgtcta acaccttctc ttctgaagaa     180

ctgtaccact tcttcaaact gccgaaactg tggaaagtta tcttcacctc tcgtggtaaa     240

gacaactaca tcgacaacat caaagctctg ttcctgaacg aactgaaagt tgctgaactg     300

gacaaccacg ctatctctaa ctctgctctg atcgaacagc cgcagctgat ctggaacctg     360

cgttctgaca tcgcttacaa cccgctgacc cagaaagctt acaaaaacaa ctaccagcac     420

aaagaaaccg gtatcatcat cgactacttc tctgacctgg ctcgtggtga acagcgtgac     480

atcccgtggg gtgacaccgg tatctctcag tgcctgatcg acaacaccgc tccgaaagtt     540

acctctgcta tcgactacat ccagtctgtt tctccggctt gcttcaacgt tctgaaagct     600

tctatctact ctatcgttgt tcgtttcgac accaccaaaa acatcttcaa ctgcgcttct     660

accaacatct gcaacggtct ggttgttctg atcaacccgc acctgaacga agtttctgtt     720

gaaaccctgg ctgacgctat cgttcacgaa atgacccaca acctgttcga catcgctgaa     780

ctgtacgaac cgtgcctgcc gaccaaattc cacccgcaga ccatcaaatc tccgtggacc     840

ggtcgtatgc tggacccgaa cacctacatc caggcttgct acacctggta cggtctgcgt     900

aacttctggc agaaagctta caaacacttc aacaccgaaa acgctcacaa atacctgcag     960

caggcttcta aaggtttcga acaggctgaa ttcgttcgta tcgctaaaaa ctctgacgaa    1020

atcatcaaca ccaaactgat caccaccctg gaacgtctga aataa                    1065


<210> 31
<211> 58
<212> PRT
<213> Photorhabdus


<220> 
<223> Photorhabdus temperata HGB1456

<400> 31
Met His Asn Thr Ser Ile Ile Asn Cys Thr Thr Gln Glu Ala Leu Asn 
1               5                   10                  15      
Ser Leu Ala Ala Ser Phe Lys Asp Thr Glu Leu Ser Ile Thr Glu Arg 
            20                  25                  30          
Ala Leu Asp Glu Leu Asn Asn Lys Pro Lys Ile Pro Glu Ile Thr Ala 
        35                  40                  45              
Trp Asn Trp Ser Lys Ser Phe Gln Glu Ile 
    50                  55              

<210> 32
<211> 58
<212> PRT
<213> Photorhabdus


<220> 
<223> Photorhabdus khanii DSM3369

<400> 32
Met His Asn Thr Leu Asn Glu Thr Val Lys Thr Gln Glu Ala Leu Asn 
1               5                   10                  15      
Ser Leu Ala Ala Ser Phe Lys Glu Thr Glu Leu Ser Ile Thr Asp Lys 
            20                  25                  30          
Ala Leu Asn Glu Leu Ser Asn Lys Pro Lys Ile Pro Glu Ile Thr Ala 
        35                  40                  45              
Trp Asn Trp Ser Lys Ser Phe Gln Glu Ile 
    50                  55              

<210> 33
<211> 58
<212> PRT
<213> Photorhabdus


<220> 
<223> Photorhabdus australis PB68.1

<400> 33
Met Gln Asn Thr Leu Val Glu Thr Cys Lys Thr Gln Glu Ala Leu Asn 
1               5                   10                  15      
Ser Leu Ala Ala Ser Phe Lys Glu Thr Glu Leu Ser Ile Thr Glu Lys 
            20                  25                  30          
Ala Leu Asn Glu Leu Ser Ser Lys Pro Lys Ile Pro Glu Ile Thr Ala 
        35                  40                  45              
Trp Asn Trp Ser Lys Ser Phe Gln Glu Ile 
    50                  55              

<210> 34
<211> 58
<212> PRT
<213> Photorhabdus


<220> 
<223> Photorhabdus laumondii BOJ-47

<400> 34
Met His Asn Thr Ser Ile Ile Asn Cys Thr Thr Gln Glu Ala Leu Asn 
1               5                   10                  15      
Ser Leu Ala Ala Ser Phe Lys Asp Thr Glu Leu Ser Ile Thr Glu Arg 
            20                  25                  30          
Ala Leu Asp Glu Leu Asn Asn Lys Pro Lys Ile Pro Glu Ile Thr Ala 
        35                  40                  45              
Trp Asn Trp Ser Lys Ser Phe Gln Glu Ile 
    50                  55              

<210> 35
<211> 66
<212> PRT
<213> Yersinia frederiksenii


<220> 
<223> Yersinia frederiksenii ATCC33641

<400> 35
Met His Thr Ser His Gln Pro Asp Lys Lys Thr Gly Asn Thr His Leu 
1               5                   10                  15      
Ile Thr Leu Lys Thr Lys Leu Glu Ser Leu Glu Glu Ser Phe Lys Asn 
            20                  25                  30          
Ser Ser Leu Ser Ile Asn Asp His Glu Ile Glu Ser Leu Lys Asn Ser 
        35                  40                  45              
Asp Ser Asp Asn Lys Ile Thr Ala Trp Asn Trp Ser Lys Ser Phe Thr 
    50                  55                  60                  
Gln Gln 
65      

<210> 36
<211> 55
<212> PRT
<213> Vibrio tasmaniensis


<220> 
<223> Vibrio tasmaniensis 10N.222.51.A7

<400> 36
Met Ile Ile Val Glu Lys Glu Lys Val Ser Ile Ser Glu Arg Leu Asp 
1               5                   10                  15      
Ala Leu Met Ser Ser Phe Ser Glu Met Asn Leu Glu Leu Thr Lys Phe 
            20                  25                  30          
Asp Gln Glu Gln Val Asn Ser Ile Asn Ile Ala Pro Pro Ile Thr Ala 
        35                  40                  45              
Trp Asn Trp Ser Lys Ser Phe 
    50                  55  

<210> 37
<211> 58
<212> PRT
<213> Pseudoalteromonas luteoviolacea


<220> 
<223> Pseudoalteromonas luteoviolacea S4054

<400> 37
Met Ile Val Glu Ala Pro Lys Glu Lys Val Ser Ile Ser Glu Lys Leu 
1               5                   10                  15      
Asp Ala Leu Lys Ser Ser Phe Ser Asn Gln Thr Leu Asn Ile Ala Asn 
            20                  25                  30          
Val Asp Gln Ala Arg Val Asp Ser Ile Ser Val Ala Pro Pro Ile Thr 
        35                  40                  45              
Ala Trp Asn Trp Ser Lys Ser Phe Glu Lys 
    50                  55              

