                         SEQUENCE LISTING

<110>  TRIM-EDICINE, INC.
 
<120>  TREATMENT OF INFLAMMATORY BOWEL DISEASE AND RADIATION-INDUCED 
       INJURY

<130>  TRIM-41-PCT

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  1509
<212>  DNA
<213>  Artificial sequence

<220>
<223>  expression module for human MG53 in plasmid of SEQ ID NO 4

<400>  1
cctaggatga aaaaaaagat tatctcagct attttaatgt ctacagtgat actttcggct       60

gcgcccggcc tcctgcacca ggagctgtcc tgcccgctgt gcctgcagct gttcgacgcg      120

cccgtgacag ccgagtgcgg ccacagtttc tgccgcgcct gcctaggccg cgtggccggg      180

gagccggcgg cggatggcac cgttctctgc ccctgctgcc aggcccccac gcggccgcag      240

gcactcagca ccaacctgca gctggcgcgc ctggtggagg ggctggccca ggtgccgcag      300

ggccactgcg aggagcacct ggacccgctg agcatctact gcgagcagga ccgcgcgctg      360

gtgtgcggag tgtgcgcctc actcggctcg caccgcggtc atcgcctcct gcctgccgcc      420

gaggcccacg cacgcctcaa gacacagctg ccacagcaga aactgcagct gcaggaggca      480

tgcatgcgca aggagaagag tgtggctgtg ctggagcatc agctggtgga ggtggaggag      540

acagtgcgtc agttccgggg ggccgtgggg gagcagctgg gcaagatgcg ggtgttcctg      600

gctgcactgg agggctcctt ggaccgcgag gcagagcgtg tacggggtga ggcaggggtc      660

gccttgcgcc gggagctggg gagcctgaac tcttacctgg agcagctgcg gcagatggag      720

aaggtcctgg aggaggtggc ggacaagccg cagactgagt tcctcatgaa atactgcctg      780

gtgaccagca ggctgcagaa gatcctggca gagtctcccc cacccgcccg tctggacatc      840

cagctgccaa ttatctcaga tgacttcaaa ttccaggtgt ggaggaagat gttccgggct      900

ctgatgccag cgctggagga gctgaccttt gacccgagct ctgcgcaccc gagcctggtg      960

gtgtcttcct ctggccgccg cgtggagtgc tcggagcaga aggcgccgcc ggccggggag     1020

gacccgcgcc agttcgacaa ggcggtggcg gtggtggcgc accagcagct ctccgagggc     1080

gagcactact gggaggtgga tgttggcgac aagccgcgct gggcgctggg cgtgatcgcg     1140

gccgaggccc cccgccgcgg gcgcctgcac gcggtgccct cgcagggcct gtggctgctg     1200

gggctgcgcg agggcaagat cctggaggca cacgtggagg ccaaggagcc gcgcgctctg     1260

cgcagccccg agaggcggcc cacgcgcatt ggcctttacc tgagcttcgg cgacggcgtc     1320

ctctccttct acgatgccag cgacgccgac gcgctcgtgc cgctttttgc cttccacgag     1380

cgcctgccca ggcccgtgta ccccttcttc gacgtgtgct ggcacgacaa gggcaagaat     1440

gcccagccgc tgctgctcgt gggtcccgaa ggcgccgagg cccaccacca ccatcaccac     1500

tgaggtacc                                                             1509


<210>  2
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  upstream primer

<400>  2
caccgttctc tgcccctg                                                     18


<210>  3
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  downstream primer

<400>  3
ctgtgtcttg aggcgtgc                                                     18


<210>  4
<211>  4649
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SP-MG53-6xHis in the pNZ8148 plasmid

<400>  4
agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa       60

ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg      120

gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa      180

taaattataa ggaggcactc acctaggatg aaaaaaaaga ttatctcagc tattttaatg      240

tctacagtga tactttcggc tgcgcccggc ctcctgcacc aggagctgtc ctgcccgctg      300

tgcctgcagc tgttcgacgc gcccgtgaca gccgagtgcg gccacagttt ctgccgcgcc      360

tgcctaggcc gcgtggccgg ggagccggcg gcggatggca ccgttctctg cccctgctgc      420

caggccccca cgcggccgca ggcactcagc accaacctgc agctggcgcg cctggtggag      480

gggctggccc aggtgccgca gggccactgc gaggagcacc tggacccgct gagcatctac      540

tgcgagcagg accgcgcgct ggtgtgcgga gtgtgcgcct cactcggctc gcaccgcggt      600

catcgcctcc tgcctgccgc cgaggcccac gcacgcctca agacacagct gccacagcag      660

aaactgcagc tgcaggaggc atgcatgcgc aaggagaaga gtgtggctgt gctggagcat      720

cagctggtgg aggtggagga gacagtgcgt cagttccggg gggccgtggg ggagcagctg      780

ggcaagatgc gggtgttcct ggctgcactg gagggctcct tggaccgcga ggcagagcgt      840

gtacggggtg aggcaggggt cgccttgcgc cgggagctgg ggagcctgaa ctcttacctg      900

gagcagctgc ggcagatgga gaaggtcctg gaggaggtgg cggacaagcc gcagactgag      960

ttcctcatga aatactgcct ggtgaccagc aggctgcaga agatcctggc agagtctccc     1020

ccacccgccc gtctggacat ccagctgcca attatctcag atgacttcaa attccaggtg     1080

tggaggaaga tgttccgggc tctgatgcca gcgctggagg agctgacctt tgacccgagc     1140

tctgcgcacc cgagcctggt ggtgtcttcc tctggccgcc gcgtggagtg ctcggagcag     1200

aaggcgccgc cggccgggga ggacccgcgc cagttcgaca aggcggtggc ggtggtggcg     1260

caccagcagc tctccgaggg cgagcactac tgggaggtgg atgttggcga caagccgcgc     1320

tgggcgctgg gcgtgatcgc ggccgaggcc ccccgccgcg ggcgcctgca cgcggtgccc     1380

tcgcagggcc tgtggctgct ggggctgcgc gagggcaaga tcctggaggc acacgtggag     1440

gccaaggagc cgcgcgctct gcgcagcccc gagaggcggc ccacgcgcat tggcctttac     1500

ctgagcttcg gcgacggcgt cctctccttc tacgatgcca gcgacgccga cgcgctcgtg     1560

ccgctttttg ccttccacga gcgcctgccc aggcccgtgt accccttctt cgacgtgtgc     1620

tggcacgaca agggcaagaa tgcccagccg ctgctgctcg tgggtcccga aggcgccgag     1680

gcccaccacc accatcacca ctgaggtacc actagttcta gagagctcaa gctttctttg     1740

aaccaaaatt agaaaaccaa ggcttgaaac gttcaattga aatggcaatt aaacaaatta     1800

cagcacgtgt tgctttgatt gatagccaaa aagcagcagt tgataaagca attactgata     1860

ttgctgaaaa attgtaattt ataaataaaa atcacctttt agaggtggtt tttttattta     1920

taaattattc gtttgatttc gctttcgata gaacaatcaa atcgtttctg agacgtttta     1980

gcgtttattt cgtttagtta tcggcataat cgttaaaaca ggcgttatcg tagcgtaaaa     2040

gcccttgagc gtagcgtggc tttgcagcga agatgttgtc tgttagatta tgaaagccga     2100

tgactgaatg aaataataag cgcagcgtcc ttctatttcg gttggaggag gctcaaggga     2160

gtttgaggga atgaaattcc ctcatgggtt tgattttaaa aattgcttgc aattttgccg     2220

agcggtagcg ctggaaaatt tttgaaaaaa atttggaatt tggaaaaaaa tggggggaaa     2280

ggaagcgaat tttgcttccg tactacgacc ccccattaag tgccgagtgc caatttttgt     2340

gccaaaaacg ctctatccca actggctcaa gggtttgagg ggtttttcaa tcgccaacga     2400

atcgccaacg ttttcgccaa cgttttttat aaatctatat ttaagtagct ttatttttgt     2460

ttttatgatt acaaagtgat acactaattt tataaaatta tttgattgga gttttttaaa     2520

tggtgatttc agaatcgaaa aaaagagtta tgatttctct gacaaaagag caagataaaa     2580

aattaacaga tatggcgaaa caaaaagatt tttcaaaatc tgcggttgcg gcgttagcta     2640

tagaagaata tgcaagaaag gaatcagaac aaaaaaaata agcgaaagct cgcgttttta     2700

gaaggatacg agttttcgct acttgttttt gataaggtaa ttatatcatg gctattaaaa     2760

atactaaagc tagaaatttt ggatttttat tatatcctga ctcaattcct aatgattgga     2820

aagaaaaatt agagagtttg ggcgtatcta tggctgtcag tcctttacac gatatggacg     2880

aaaaaaaaga taaagataca tggaatagta gtgatgttat acgaaatgga aagcactata     2940

aaaaaccaca ctatcacgtt atatatattg cacgaaatcc tgtaacaata gaaagcgtta     3000

ggaacaagat taagcgaaaa ttggggaata gttcagttgc tcatgttgag atacttgatt     3060

atatcaaagg ttcatatgaa tatttgactc atgaatcaaa ggacgctatt gctaagaata     3120

aacatatata cgacaaaaaa gatattttga acattaatga ttttgatatt gaccgctata     3180

taacacttga tgaaagccaa aaaagagaat tgaagaattt acttttagat atagtggatg     3240

actataattt ggtaaataca aaagatttaa tggcttttat tcgccttagg ggagcggagt     3300

ttggaatttt aaatacgaat gatgtaaaag atattgtttc aacaaactct agcgccttta     3360

gattatggtt tgagggcaat tatcagtgtg gatatagagc aagttatgca aaggttcttg     3420

atgctgaaac gggggaaata aaatgacaaa caaagaaaaa gagttatttg ctgaaaatga     3480

ggaattaaaa aaagaaatta aggacttaaa agagcgtatt gaaagataca gagaaatgga     3540

agttgaatta agtacaacaa tagatttatt gagaggaggg attattgaat aaataaaagc     3600

ccccctgacg aaagtcgacg gcaatagtta cccttattat caagataaga aagaaaagga     3660

tttttcgcta cgctcaaatc ctttaaaaaa acacaaaaga ccacattttt taatgtggtc     3720

ttttattctt caactaaagc acccattagt tcaacaaacg aaaattggat aaagtgggat     3780

atttttaaaa tatatattta tgttacagta atattgactt ttaaaaaagg attgattcta     3840

atgaagaaag cagacaagta agcctcctaa attcacttta gataaaaatt taggaggcat     3900

atcaaatgaa ctttaataaa attgatttag acaattggaa gagaaaagag atatttaatc     3960

attatttgaa ccaacaaacg acttttagta taaccacaga aattgatatt agtgttttat     4020

accgaaacat aaaacaagaa ggatataaat tttaccctgc atttattttc ttagtgacaa     4080

gggtgataaa ctcaaataca gcttttagaa ctggttacaa tagcgacgga gagttaggtt     4140

attgggataa gttagagcca ctttatacaa tttttgatgg tgtatctaaa acattctctg     4200

gtatttggac tcctgtaaag aatgacttca aagagtttta tgatttatac ctttctgatg     4260

tagagaaata taatggttcg gggaaattgt ttcccaaaac acctatacct gaaaatgctt     4320

tttctctttc tattattcct tggacttcat ttactgggtt taacttaaat atcaataata     4380

atagtaatta ccttctaccc attattacag caggaaaatt cattaataaa ggtaattcaa     4440

tatatttacc gctatcttta caggtacatc attctgtttg tgatggttat catgctggat     4500

tgtttatgaa ctctattcag gaattgtcag ataggcctaa tgactggctt ttataatatg     4560

agataatgcc gactgtactt tttacagtcg gttttctaat gtcactaacc tgccccgtta     4620

gttgaagaag gtttttatat tacagctcc                                       4649


<210>  5
<211>  1569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression module of MG53 as part of plasmid in SEQ ID NO 4

<400>  5
ggcgagctca aatgcaacgt aaaaagaaag gtttaagtat cttattagct ggtacagttg       60

ctttaggtgc tttagctgtt ttaccagttg gcgaaatcca agcaaaggct gacacaaaca      120

gtgatatgag tgctgctcca ggcttattac accaggaatt aagttgcccg ttatgcttac      180

agttattcga cgctccagtt acagctgaat gcggccacag tttctgccgt gcttgcttag      240

gccgtgttgc tggtgaaccg gctgctgatg gcaccgtttt atgcccatgc tgccaggctc      300

caacccgtcc gcaggcatta agtaccaact tacagttagc tcgtttagtt gaaggtttag      360

ctcaggttcc gcagggccac tgcgaagaac acttagaccc gttaagtatc tactgcgaac      420

aggaccgtgc tttagtttgc ggtgtttgcg cttcattagg cagtcaccgt ggtcatcgtt      480

tattaccagc tgctgaagct cacgcacgtt taaagacaca gttaccacag cagaaattac      540

agttacagga agcatgcatg cgtaaggaaa agagtgttgc tgttttagaa catcagttag      600

ttgaagttga agaaacagtt cgtcagttcc gtggtgctgt tggtgaacag ttaggcaaga      660

tgcgtgtttt cttagctgca ttagaaggca gtttagaccg tgaagcagaa cgtgttcgtg      720

gtgaagcagg tgttgcttta cgtcgtgaat taggtagttt aaacagttac ttagaacagt      780

tacgtcagat ggaaaaggtt ttagaagaag ttgctgacaa gccgcagacc gaattcttaa      840

tgaaatactg cttagttacc agtcgtttac agaagatctt agcagaaagt ccaccaccag      900

ctcgtttaga catccagtta ccaattatct cagatgactt caaattccag gtttggcgta      960

agatgttccg tgctttaatg ccagctttag aagaattaac ctttgacccg agtagtgctc     1020

acccgagttt agttgttagt agtagtggcc gtcgtgttga atgcagtgaa cagaaggctc     1080

cgccggctgg tgaagacccg cgtcagttcg acaaggctgt tgctgttgtt gctcaccagc     1140

agttaagtga aggcgaacac tactgggaag ttgatgttgg cgacaagccg cgttgggctt     1200

taggcgttat cgctgctgaa gctccacgtc gtggtcgttt acacgctgtt ccaagtcagg     1260

gcttatggtt attaggttta cgtgaaggca agatcttaga agcacacgtt gaagctaagg     1320

aaccgcgtgc tttacgtagt ccagaacgtc gtccaacccg tattggctta tacttaagtt     1380

tcggcgacgg cgttttaagt ttctacgatg ctagtgacgc tgacgcttta gttccgttat     1440

ttgctttcca cgaacgttta ccacgtccag tttacccatt cttcgacgtt tgctggcacg     1500

acaagggcaa gaatgctcag ccgttattat tagttggtcc agaaggcgct gaagcttaag     1560

tcgacgcgt                                                             1569


<210>  6
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide SP-prtP cell wall associate protease

<400>  6

Met Gln Arg Lys Lys Lys Gly Leu Ser Ile Leu Leu Ala Gly Thr Val 
1               5                   10                  15      


Ala Leu Gly Ala Leu Ala Val Leu Pro Val Gly Glu Ile Gln Ala Lys 
            20                  25                  30          


Ala Asp Thr Asn Ser Asp 
        35              


<210>  7
<211>  114
<212>  DNA
<213>  Artificial sequence

<220>
<223>  signal peptide SP-prtP; cell wall associated protease

<400>  7
atgcaacgta aaaagaaagg tttaagtatc ttattagctg gtacagttgc tttaggtgct       60

ttagctgttt taccagttgg cgaaatccaa gcaaaggctg acacaaacag tgat            114


<210>  8
<211>  36
<212>  PRT
<213>  Artificial sequence

<220>
<223>  signal peptide SP-slpA; SplA, cell surface protein

<400>  8

Met Lys Lys Asn Leu Arg Ile Val Ser Ala Ala Ala Ala Ala Leu Leu 
1               5                   10                  15      


Ala Val Ala Pro Val Ala Ala Ser Ala Val Ser Val Asn Ala Ala Ala 
            20                  25                  30          


Thr Thr Thr Asn 
        35      


<210>  9
<211>  108
<212>  DNA
<213>  Artificial sequence

<220>
<223>  signal peptide SP-slpA; SplA, cell surface protein

<400>  9
atgaagaaaa atttaagaat tgttagcgct gctgctgctg ctttattagc tgttgctcca       60

gttgctgctt cagctgtttc tgttaacgct gctgcaacca ctaccaac                   108


