       

<110>            /Skolkovo Institute of Science and Technology
<120>  CAS9    PASTEURELLA PNEUMOTROPICA      

<130> 424316
<150> RU 2019136164
<151> 2019-11-11

<160> 2

<210> 1
<211> 1055
<212> PRT
<213> Pasteurella pneumotropica


<220> 
<223> ,  Cas9

<400> 1
Met Gln Asn Asn Pro Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 
1               5                   10                  15      
Ala Ser Ile Gly Trp Ala Val Val Glu Ile Asp Glu Glu Ser Ser Pro 
            20                  25                  30          
Ile Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg Ala Glu Val 
        35                  40                  45              
Ala Lys Thr Gly Glu Ser Leu Ala Leu Ser Arg Arg Leu Ala Arg Ser 
    50                  55                  60                  
Ser Arg Arg Leu Ile Lys Arg Arg Ala Glu Arg Leu Lys Lys Ala Lys 
65                  70                  75                  80  
Arg Leu Leu Lys Ala Glu Lys Ile Leu His Ser Ile Asp Glu Lys Leu 
                85                  90                  95      
Pro Ile Asn Val Trp Gln Leu Arg Val Lys Gly Leu Lys Glu Lys Leu 
            100                 105                 110         
Glu Arg Gln Glu Trp Ala Ala Val Leu Leu His Leu Ser Lys His Arg 
        115                 120                 125             
Gly Tyr Leu Ser Gln Arg Lys Asn Glu Gly Lys Ser Asp Asn Lys Glu 
    130                 135                 140                 
Leu Gly Ala Leu Leu Ser Gly Ile Ala Ser Asn His Gln Met Leu Gln 
145                 150                 155                 160 
Ser Ser Glu Tyr Arg Thr Pro Ala Glu Ile Ala Val Lys Lys Phe Gln 
                165                 170                 175     
Val Glu Glu Gly His Ile Arg Asn Gln Arg Gly Ser Tyr Thr His Thr 
            180                 185                 190         
Phe Ser Arg Leu Asp Leu Leu Ala Glu Met Glu Leu Leu Phe Gln Arg 
        195                 200                 205             
Gln Ala Glu Leu Gly Asn Ser Tyr Thr Ser Thr Thr Leu Leu Glu Asn 
    210                 215                 220                 
Leu Thr Ala Leu Leu Met Trp Gln Lys Pro Ala Leu Ala Gly Asp Ala 
225                 230                 235                 240 
Ile Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Pro Ser Glu Tyr Lys 
                245                 250                 255     
Ala Ala Lys Asn Ser Tyr Ser Ala Glu Arg Phe Val Trp Leu Thr Lys 
            260                 265                 270         
Leu Asn Asn Leu Arg Ile Leu Glu Asn Gly Thr Glu Arg Ala Leu Asn 
        275                 280                 285             
Asp Asn Glu Arg Phe Ala Leu Leu Glu Gln Pro Tyr Glu Lys Ser Lys 
    290                 295                 300                 
Leu Thr Tyr Ala Gln Val Arg Ala Met Leu Ala Leu Ser Asp Asn Ala 
305                 310                 315                 320 
Ile Phe Lys Gly Val Arg Tyr Leu Gly Glu Asp Lys Lys Thr Val Glu 
                325                 330                 335     
Ser Lys Thr Thr Leu Ile Glu Met Lys Phe Tyr His Gln Ile Arg Lys 
            340                 345                 350         
Thr Leu Gly Ser Ala Glu Leu Lys Lys Glu Trp Asn Glu Leu Lys Gly 
        355                 360                 365             
Asn Ser Asp Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys 
    370                 375                 380                 
Thr Asp Asp Asp Ile Cys Arg Tyr Leu Glu Gly Lys Leu Pro Glu Arg 
385                 390                 395                 400 
Val Leu Asn Ala Leu Leu Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln 
                405                 410                 415     
Leu Ser Leu Lys Ala Leu His Gln Ile Leu Pro Leu Met Leu Gln Gly 
            420                 425                 430         
Gln Arg Tyr Asp Glu Ala Val Ser Ala Ile Tyr Gly Asp His Tyr Gly 
        435                 440                 445             
Lys Lys Ser Thr Glu Thr Thr Arg Leu Leu Pro Thr Ile Pro Ala Asp 
    450                 455                 460                 
Glu Ile Arg Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys 
465                 470                 475                 480 
Val Ile Asn Ala Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His 
                485                 490                 495     
Ile Glu Thr Ala Arg Glu Val Gly Lys Ser Tyr Gln Asp Arg Lys Lys 
            500                 505                 510         
Leu Glu Lys Gln Gln Glu Asp Asn Arg Lys Gln Arg Glu Ser Ala Val 
        515                 520                 525             
Lys Lys Phe Lys Glu Met Phe Pro His Phe Val Gly Glu Pro Lys Gly 
    530                 535                 540                 
Lys Asp Ile Leu Lys Met Arg Leu Tyr Glu Leu Gln Gln Ala Lys Cys 
545                 550                 555                 560 
Leu Tyr Ser Gly Lys Ser Leu Glu Leu His Arg Leu Leu Glu Lys Gly 
                565                 570                 575     
Tyr Val Glu Val Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp 
            580                 585                 590         
Ser Phe Asn Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys 
        595                 600                 605             
Gly Asn Leu Thr Pro Tyr Glu Trp Leu Asp Gly Lys Asn Asn Ser Glu 
    610                 615                 620                 
Arg Trp Gln His Phe Val Val Arg Val Gln Thr Ser Gly Phe Ser Tyr 
625                 630                 635                 640 
Ala Lys Lys Gln Arg Ile Leu Asn His Lys Leu Asp Glu Lys Gly Phe 
                645                 650                 655     
Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys 
            660                 665                 670         
Asn Phe Ile Ala Asp Asn Met Leu Leu Val Gly Lys Gly Lys Arg Asn 
        675                 680                 685             
Val Phe Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg His Arg Trp 
    690                 695                 700                 
Gly Leu Gln Lys Val Arg Glu Gln Asn Asp Arg His His Ala Leu Asp 
705                 710                 715                 720 
Ala Val Val Val Ala Cys Ser Thr Val Ala Met Gln Gln Lys Ile Thr 
                725                 730                 735     
Arg Phe Val Arg Tyr Asn Glu Gly Asn Val Phe Ser Gly Glu Arg Ile 
            740                 745                 750         
Asp Arg Glu Thr Gly Glu Ile Ile Pro Leu His Phe Pro Ser Pro Trp 
        755                 760                 765             
Ala Phe Phe Lys Glu Asn Val Glu Ile Arg Ile Phe Ser Glu Asn Pro 
    770                 775                 780                 
Lys Leu Glu Leu Glu Asn Arg Leu Pro Asp Tyr Pro Gln Tyr Asn His 
785                 790                 795                 800 
Glu Trp Val Gln Pro Leu Phe Val Ser Arg Met Pro Thr Arg Lys Met 
                805                 810                 815     
Thr Gly Gln Gly His Met Glu Thr Val Lys Ser Ala Lys Arg Leu Asn 
            820                 825                 830         
Glu Gly Leu Ser Val Leu Lys Val Pro Leu Thr Gln Leu Lys Leu Ser 
        835                 840                 845             
Asp Leu Glu Arg Met Val Asn Arg Asp Arg Glu Ile Ala Leu Tyr Glu 
    850                 855                 860                 
Ser Leu Lys Ala Arg Leu Glu Gln Phe Gly Asn Asp Pro Ala Lys Ala 
865                 870                 875                 880 
Phe Ala Glu Pro Phe Tyr Lys Lys Gly Gly Ala Leu Val Lys Ala Val 
                885                 890                 895     
Arg Leu Glu Gln Thr Gln Lys Ser Gly Val Leu Val Arg Asp Gly Asn 
            900                 905                 910         
Gly Val Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Thr Lys 
        915                 920                 925             
Gly Gly Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys 
    930                 935                 940                 
Gly Ile Leu Pro Asn Arg Ala Ala Thr Gln Gly Lys Asp Glu Asn Asp 
945                 950                 955                 960 
Trp Asp Ile Met Asp Glu Met Ala Thr Phe Gln Phe Ser Leu Cys Gln 
                965                 970                 975     
Asn Asp Leu Ile Lys Leu Val Thr Lys Lys Lys Thr Ile Phe Gly Tyr 
            980                 985                 990         
Phe Asn Gly Leu Asn Arg Ala Thr Ser Asn Ile Asn Ile Lys Glu His 
        995                 1000                1005            
Asp Leu Asp Lys Ser Lys Gly Lys Leu Gly Ile Tyr Leu Glu Val Gly 
    1010                1015                1020                
Val Lys Leu Ala Ile Ser Leu Glu Lys Tyr Gln Val Asp Glu Leu Gly 
1025                1030                1035                1040
Lys Asn Ile Arg Pro Cys Arg Pro Thr Lys Arg Gln His Val Arg 
                1045                1050                1055

<210> 2
<211> 3168
<212> DNA
<213> Pasteurella pneumotropica


<220> 
<223> ,  Cas9

<400> 2
atgcaaaata atccattaaa ttacatttta gggttagatt taggcattgc ttctattggt       60

tgggcggttg tggaaattga tgaggagagt tcacctattc gcttaattga tgtgggcgtc      120

cgtacatttg aacgggctga agtcgctaaa accggcgaaa gtttagcatt gtctcgtcgt      180

ttagctcgtt catcacggcg attaattaaa cgccgagcag agcgattaaa aaaagcaaaa      240

cgtttattaa aagcagaaaa gattttacat tctattgatg aaaaattacc cattaatgtt      300

tggcagcttc gagtaaaagg attgaaggaa aaactcgaac gtcaggagtg ggcagcggtt      360

ttattacatt tgtcaaagca tcgtggctat ttatcacaac gtaaaaatga gggtaaaagt      420

gataataaag agctgggggc attactttca ggtatcgcaa gtaaccacca aatgttgcaa      480

tcctccgaat atcgtacccc tgcagaaatt gcagtcaaaa aatttcaagt agaagaagga      540

catattcgta atcaacgtgg atcttatacc cataccttta gccgtttgga tttgttggca      600

gaaatggaat tattatttca acgccaagct gagttaggca attcttacac gtccaccaca      660

ttattagaaa atttgacggc gttactaatg tggcaaaagc cagctcttgc gggtgatgcg      720

attttaaaaa tgttgggcaa gtgtaccttc gaacccagcg aatataaagc cgcaaaaaat      780

agttattctg ctgaacgttt tgtgtggtta accaagctga ataatttacg cattttagaa      840

aatggcacgg aaagagcttt aaatgacaat gaacgttttg ctttgcttga gcaaccgtat      900

gagaaatcaa aattaactta tgctcaagtg agagcaatgc ttgcgttatc tgataatgct      960

attttcaaag gggttcgtta tttaggcgaa gataaaaaaa cagtagagag caaaactacg     1020

ttgatagaaa tgaagtttta tcatcaaatc cgcaaaacat taggcagtgc agaattaaaa     1080

aaggaatgga atgagttaaa aggcaattcc gatttattag atgagattgg cacggcattt     1140

tcgttgtata aaacggatga tgatatttgc cgttatttag agggaaaact accagaaagg     1200

gtattaaatg cgttattgga aaatttaaat ttcgataaat ttattcaact ttcacttaaa     1260

gccttacacc aaattttacc attgatgctg caagggcaac gttatgatga ggcggtttct     1320

gcgatttatg gtgatcatta tggtaaaaaa tcgacagaaa caacccgctt gttgccgact     1380

attcctgccg atgaaatccg aaatcctgtg gtattacgca ccctgaccca agcccgtaaa     1440

gtgatcaatg cggtggtgcg gttatatggt tcgcctgccc gtattcatat tgaaacagcg     1500

agagaagtcg gcaaatctta ccaagatcgt aaaaaacttg aaaaacagca agaagataat     1560

cgtaagcaac gtgaaagtgc ggtcaaaaaa tttaaagaaa tgtttccgca ttttgtgggg     1620

gagccgaaag gtaaagatat tttaaaaatg cgattgtatg agttacaaca agcgaaatgt     1680

ttatattctg gaaaatcttt agaacttcat cgtttgcttg agaaggggta tgtagaagtg     1740

gatcacgctt tgccattttc tcgcacgtgg gatgatagct ttaataataa agtactggtg     1800

cttgccaacg agaaccaaaa taaaggcaat ttaacgcctt atgaatggtt agatggtaaa     1860

aataacagtg agcgttggca acattttgtt gtacgagtac aaaccagcgg tttctcttat     1920

gctaaaaaac aacgcatttt gaaccataaa ttggatgaaa aagggtttat cgaacgtaat     1980

ttaaacgata ctcgctatgt agctcgtttc ttatgtaact ttattgccga taatatgttg     2040

ttggttggta aaggcaagcg aaacgtgttt gcttcaaacg ggcaaatcac ggcgttattg     2100

cggcatcgtt ggggcttaca aaaagtgcgt gaacagaatg atcgccacca cgcactggac     2160

gcggttgtgg tggcttgctc tactgtggca atgcaacaaa aaatcactcg atttgtgaga     2220

tataacgaag gaaatgtctt tagcggtgaa cgtatcgatc gtgaaactgg cgagattatt     2280

ccattacatt ttccaagccc ttgggctttt ttcaaagaga atgtggaaat tcgcattttt     2340

agtgaaaatc caaaattgga attagaaaat cgcctgcctg attatccgca atataatcac     2400

gaatgggtgc aaccattgtt tgtttcgaga atgccaaccc gaaaaatgac agggcaaggg     2460

catatggaaa cggtaaaatc cgcaaaacga ttaaatgaag gtttaagtgt gttaaaagtc     2520

cctttaacac aacttaaatt gagtgattta gaacgaatgg ttaatcgtga tcgtgaaatt     2580

gcattgtatg aatccttaaa agcacgttta gagcaatttg gtaacgaccc agccaaagcc     2640

tttgccgaac cattctataa aaagggtggg gcattagtca aagcagtccg attggaacaa     2700

acacaaaaat cgggggtatt agtacgtgat ggtaacggtg ttgcggataa tgcttcaatg     2760

gtacgggttg atgtttttac taaaggtgga aaatatttct tagtgccgat ttatacttgg     2820

caggtagcga aagggatttt accgaatagg gctgcgacac aaggtaaaga tgaaaatgat     2880

tgggatatta tggatgaaat ggctactttc caattttctc tatgtcaaaa tgatctaatt     2940

aaattagtta ccaaaaagaa aacaatcttt ggatatttta atggattaaa tagagctact     3000

agcaatataa atattaaaga gcatgatcta gataagtcta aagggaaatt aggtatttac     3060

ttagaagttg gtgtaaaact agctatttcc cttgaaaagt accaagtcga cgaactcggc     3120

aaaaatatcc gtccttgtcg tccgactaaa cgacagcacg tgcgttaa                  3168
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