                         SEQUENCE LISTING

<110>  WuXi Biologics (Shanghai) CO., LTD
        WuXi Biologics Ireland Limited
 
<120>  A novel antibody against TIGIT

<130>  AJ3297PCT2010

<150>  PCT/CN2019/118522
<151>  2019-11-14

<160>  14    

<170>  PatentIn version 3.3

<210>  1
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  HCDR1

<400>  1

Gly Gly Ser Ile Thr Ser Ser Ser Tyr Tyr Trp Gly 
1               5                   10          


<210>  2
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  LCDR1

<400>  2

Thr Gly Thr Ser Asn Asp Val Gly Gly Tyr Asn Tyr Val Ser 
1               5                   10                  


<210>  3
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  HCDR2

<400>  3

Ser Ile Phe Phe Ser Gly Ile Thr Tyr Tyr Asn Pro Ser Leu Lys Ser 
1               5                   10                  15      


<210>  4
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  LCDR2

<400>  4

Asp Val Ser Asn Arg Arg Thr 
1               5           


<210>  5
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  HCDR3

<400>  5

Arg Gly Leu Gly Gly Ile Leu Ala Ser Gly Tyr Phe Asp Tyr 
1               5                   10                  


<210>  6
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  LCDR3

<400>  6

Ser Ser Tyr Thr Ser Ile Ser Thr Leu Val Val 
1               5                   10      


<210>  7
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH

<400>  7

Gln Leu Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser Ile Thr Ser Ser 
            20                  25                  30          


Ser Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ser Ile Phe Phe Ser Gly Ile Thr Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Val Thr Val Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  


Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Arg Gly Leu Gly Gly Ile Leu Ala Ser Gly Tyr Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  8
<211>  111
<212>  PRT
<213>  Artificial

<220>
<223>  VL

<400>  8

Gln Ser Ala Leu Thr Gln Pro Ala Ser Val Ser Gly Ser Pro Gly Gln 
1               5                   10                  15      


Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Asn Asp Val Gly Gly Tyr 
            20                  25                  30          


Asn Tyr Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Arg Leu 
        35                  40                  45              


Leu Ile Phe Asp Val Ser Asn Arg Arg Thr Gly Ile Ser Asn Arg Phe 
    50                  55                  60                  


Ser Gly Ser Lys Ser Gly Ile Thr Ala Ser Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Tyr Thr Ser Ile 
                85                  90                  95      


Ser Thr Leu Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110     


<210>  9
<211>  372
<212>  DNA
<213>  Artificial

<220>
<223>  VH

<400>  9
caactccagc tgcaagagtc gggcccagga ctggtaaagc cttcggagac cctgtccctc     60

acctgcgctg tctctggtgg ctccatcacc agtagtagtt actactgggg ctggatccgc    120

cagcccccag ggaaggggct ggagtggatt gggagtatct tttttagtgg gatcacctac    180

tacaacccgt ccctcaagag tcgagtcacc gtatccgtag acacgtccaa gaaccagttc    240

tccctgaaac tgagctctgt gaccgccgca gacacggcta tttattactg tgcgagacgg    300

ggacttgggg gaattctcgc ctccggctac tttgactact ggggccaggg aaccctggtc    360

accgtctcct ca                                                        372


<210>  10
<211>  333
<212>  DNA
<213>  Artificial

<220>
<223>  VL

<400>  10
cagtctgccc tgactcagcc tgcctccgtg tctgggtctc ctggacagtc gatcaccata     60

tcctgcactg gaaccagcaa tgacgttggt ggttacaact atgtctcctg gtaccaacaa    120

catccaggca aagccccccg actcttgatt tttgatgtca gtaatcgacg cacagggatt    180

tccaatcgct tctctggctc caagtctggc atcacggcct ccctgaccat ctctgggctc    240

caggctgagg acgaggctga ctattactgc agttcatata caagcatcag cactctcgtg    300

gtattcggcg gagggaccaa gctgaccgtc cta                                 333


<210>  11
<211>  450
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain

<400>  11

Gln Leu Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser Ile Thr Ser Ser 
            20                  25                  30          


Ser Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ser Ile Phe Phe Ser Gly Ile Thr Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Val Thr Val Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  


Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Arg Gly Leu Gly Gly Ile Leu Ala Ser Gly Tyr Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu 
    130                 135                 140                 


Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn 
        195                 200                 205             


Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser 
    210                 215                 220                 


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly 
225                 230                 235                 240 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                245                 250                 255     


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
            260                 265                 270         


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
        275                 280                 285             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr 
    290                 295                 300                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
305                 310                 315                 320 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
                325                 330                 335     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            340                 345                 350         


Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser 
        355                 360                 365             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    370                 375                 380                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
385                 390                 395                 400 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val 
                405                 410                 415     


Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met 
            420                 425                 430         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        435                 440                 445             


Leu Gly 
    450 


<210>  12
<211>  217
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain

<400>  12

Gln Ser Ala Leu Thr Gln Pro Ala Ser Val Ser Gly Ser Pro Gly Gln 
1               5                   10                  15      


Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Asn Asp Val Gly Gly Tyr 
            20                  25                  30          


Asn Tyr Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Arg Leu 
        35                  40                  45              


Leu Ile Phe Asp Val Ser Asn Arg Arg Thr Gly Ile Ser Asn Arg Phe 
    50                  55                  60                  


Ser Gly Ser Lys Ser Gly Ile Thr Ala Ser Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Tyr Thr Ser Ile 
                85                  90                  95      


Ser Thr Leu Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 110         


Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe 
    130                 135                 140                 


Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val 
145                 150                 155                 160 


Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys 
                165                 170                 175     


Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser 
            180                 185                 190         


His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu 
        195                 200                 205             


Lys Thr Val Ala Pro Thr Glu Cys Ser 
    210                 215         


<210>  13
<211>  1350
<212>  DNA
<213>  Artificial

<220>
<223>  Heavy chain

<400>  13
caactccagc tgcaagagtc gggcccagga ctggtaaagc cttcggagac cctgtccctc     60

acctgcgctg tctctggtgg ctccatcacc agtagtagtt actactgggg ctggatccgc    120

cagcccccag ggaaggggct ggagtggatt gggagtatct tttttagtgg gatcacctac    180

tacaacccgt ccctcaagag tcgagtcacc gtatccgtag acacgtccaa gaaccagttc    240

tccctgaaac tgagctctgt gaccgccgca gacacggcta tttattactg tgcgagacgg    300

ggacttgggg gaattctcgc ctccggctac tttgactact ggggccaggg aaccctggtc    360

accgtctcct cagcgtcgac caagggccca tccgtcttcc ccctggcgcc ctgctccagg    420

agcacctccg agagcacagc cgccctgggc tgcctggtca aggactactt ccccgaaccg    480

gtgacggtgt cgtggaactc aggcgccctg accagcggcg tgcacacctt cccggctgtc    540

ctacagtcct caggactcta ctccctcagc agcgtggtga ccgtgccctc cagcagcttg    600

ggcacgaaga cctacacctg caacgtagat cacaagccca gcaacaccaa ggtggacaag    660

agagttgagt ccaaatatgg tcccccatgc ccaccatgcc cagcacctga gttcctgggg    720

ggaccatcag tcttcctgtt ccccccaaaa cccaaggaca ctctcatgat ctcccggacc    780

cctgaggtca cgtgcgtggt ggtggacgtg agccaggaag accccgaggt ccagttcaac    840

tggtacgtgg atggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagttc    900

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaacggc    960

aaggagtaca agtgcaaggt ctccaacaaa ggcctcccgt cctccatcga gaaaaccatc   1020

tccaaagcca aagggcagcc ccgagagcca caggtgtaca ccctgccccc atcccaggag   1080

gagatgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta ccccagcgac   1140

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc   1200

gtgctggact ccgacggctc cttcttcctc tacagcaggc taaccgtgga caagagcagg   1260

tggcaggagg ggaatgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac   1320

acacagaaga gcctctccct gtctctgggt                                    1350


<210>  14
<211>  651
<212>  DNA
<213>  Artificial

<220>
<223>  Light chain

<400>  14
cagtctgccc tgactcagcc tgcctccgtg tctgggtctc ctggacagtc gatcaccata     60

tcctgcactg gaaccagcaa tgacgttggt ggttacaact atgtctcctg gtaccaacaa    120

catccaggca aagccccccg actcttgatt tttgatgtca gtaatcgacg cacagggatt    180

tccaatcgct tctctggctc caagtctggc atcacggcct ccctgaccat ctctgggctc    240

caggctgagg acgaggctga ctattactgc agttcatata caagcatcag cactctcgtg    300

gtattcggcg gagggaccaa gctgaccgtc ctaggtcagc ccaaggctgc cccctcggtc    360

actctgttcc cgccctcctc tgaggagctt caagccaaca aggccacact ggtgtgtctc    420

ataagtgact tctacccggg agccgtgaca gtggcctgga aggcagatag cagccccgtc    480

aaggcgggag tggagaccac cacaccctcc aaacaaagca acaacaagta cgcggccagc    540

agctacctga gcctgacgcc tgagcagtgg aagtcccaca gaagctacag ctgccaggtc    600

acgcatgaag ggagcaccgt ggagaagaca gtggccccta cagaatgttc a             651


