                         SEQUENCE LISTING

<110>  MABPRO a.s.
 
<120>  HUMANIZED ANTI-CA IX ANTIBODIES AND METHODS OF THEIR USE

<130>  P1791PC01

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  CDR sequence 1

<400>  1

Gly Phe Thr Phe Asn Thr Asn Ala Met His 
1               5                   10  


<210>  2
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  CDR sequence 2

<400>  2

Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp Ser 
1               5                   10                  15      


Val Lys Asp 
            


<210>  3
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  CDR sequence 3

<400>  3

Val Cys Gly Ser Trp Phe Ala Tyr 
1               5               


<210>  4
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  CDR sequence 1

<400>  4

Lys Ser Ser Gln Ser Leu Leu Asn Ser Ser Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  5
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  CDR sequence 2

<400>  5

Phe Thr Ser Thr Arg Gln Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  CDR sequence 3

<400>  6

Gln Gln His Tyr Ser Ile Pro Leu Thr 
1               5                   


<210>  7
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  HC variable region - general


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  E or Q

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  L or V

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  G or R

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  K or R

<220>
<221>  VARIANT
<222>  (41)..(41)
<223>  S or P

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  K or R

<220>
<221>  VARIANT
<222>  (49)..(49)
<223>  A or G

<220>
<221>  VARIANT
<222>  (76)..(76)
<223>  D or N

<220>
<221>  VARIANT
<222>  (79)..(79)
<223>  N or S

<220>
<221>  VARIANT
<222>  (81)..(81)
<223>  A or L

<220>
<221>  VARIANT
<222>  (85)..(85)
<223>  M or V

<220>
<221>  VARIANT
<222>  (89)..(89)
<223>  K or R

<220>
<221>  VARIANT
<222>  (90)..(90)
<223>  T or A

<220>
<221>  VARIANT
<222>  (112)..(112)
<223>  L or T

<400>  7

Xaa Val Gln Leu Val Glu Ser Gly Gly Gly Xaa Val Gln Pro Gly Xaa 
1               5                   10                  15      


Ser Leu Xaa Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Asn 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Xaa Gly Xaa Gly Leu Glu Trp Val 
        35                  40                  45              


Xaa Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Xaa Ser Lys Xaa Thr 
65                  70                  75                  80  


Xaa Tyr Leu Gln Xaa Asn Ser Leu Xaa Xaa Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Cys Gly Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Xaa 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  8
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  LC variants general


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  V or I

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  V or Q

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  V or A

<220>
<221>  VARIANT
<222>  (42)..(42)
<223>  Y or F

<220>
<221>  VARIANT
<222>  (49)..(49)
<223>  S or P

<220>
<221>  VARIANT
<222>  (51)..(51)
<223>  K or N

<220>
<221>  VARIANT
<222>  (52)..(52)
<223>  L or V

<220>
<221>  VARIANT
<222>  (53)..(53)
<223>  L or V

<220>
<221>  VARIANT
<222>  (69)..(69)
<223>  S or T

<220>
<221>  VARIANT
<222>  (82)..(82)
<223>  S or N

<220>
<221>  VARIANT
<222>  (93)..(93)
<223>  Y or F

<220>
<221>  VARIANT
<222>  (109)..(109)
<223>  K or Q

<220>
<221>  VARIANT
<222>  (110)..(110)
<223>  L or V

<400>  8

Asp Xaa Xaa Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Xaa Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Xaa Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Xaa Pro Xaa Xaa Xaa Ile Tyr Phe Thr Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Xaa Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Xaa Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Xaa Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ile Pro Leu Thr Phe Gly Gln Gly Thr Xaa Xaa Glu Ile 
            100                 105                 110         


Lys 
    


<210>  9
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  HC variable 1

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Asn 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Cys Gly Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  10
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  HC variable 2

<400>  10

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Asn 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Cys Gly Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  11
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  HC variable 3

<400>  11

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Asn 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Arg Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Val Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Cys Gly Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  12
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  HC variable 4

<400>  12

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Asn 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Cys Gly Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  13
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  HC variable 5

<400>  13

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Asn 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Cys Gly Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  14
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  LC variable 1

<400>  14

Asp Val Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Phe Thr Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ile Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  15
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  LC variant 2

<400>  15

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Asn Leu Val Ile Tyr Phe Thr Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ile Pro Leu Thr Phe Gly Gln Gly Thr Gln Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  16
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  LC variant 3

<400>  16

Asp Ile Gln Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Phe Thr Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ile Pro Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  17
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  LC variant 4

<400>  17

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Val Leu Ile Tyr Phe Thr Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ile Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  18
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  LC variant 5

<400>  18

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Phe Thr Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ile Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  19
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  murine VH domain

<400>  19

Glu Val Gln Leu Val Glu Thr Gly Gly Gly Leu Val Gln Pro Lys Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Asn 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Thr Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Gln Ser Met 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Met Tyr 
                85                  90                  95      


Tyr Cys Val Cys Gly Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ala 
        115         


<210>  20
<211>  120
<212>  PRT
<213>  artificial

<220>
<223>  AGP01286

<400>  20

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Gly Ser 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Arg Ile Arg Ser Lys Ala Asn Ser Tyr Ala Thr Ala Tyr Ala Ala 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Thr Arg Leu Val Gly Ala Ile Pro Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  21
<211>  119
<212>  PRT
<213>  artificial

<220>
<223>  AEX29087

<400>  21

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Asn Phe Ser Gly Pro 
            20                  25                  30          


Ala Ile His Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Arg Ile Arg Ser Lys Ala Lys Asn Phe Ala Thr Ala Tyr Ala Ala 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Thr Thr Thr Ser Ser Ser Ile Asn Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  22
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  ACS95862

<400>  22

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Arg Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Phe Ile Arg Ser Asp Gly Ser Asn Thr Tyr Tyr Ser Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Val Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Phe Gly Gly Asp Tyr Tyr Phe Gly Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  23
<211>  115
<212>  PRT
<213>  artificial

<220>
<223>  BAC01516

<400>  23

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Arg Thr Gly Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  24
<211>  117
<212>  PRT
<213>  artificial

<220>
<223>  IGHV3-73

<400>  24

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Gly Ser 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Arg Ile Arg Ser Lys Ala Asn Ser Tyr Ala Thr Ala Tyr Ala Ala 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Thr Arg Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  25
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  murine VL domain

<400>  25

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ala Met Ser Leu Gly 
1               5                   10                  15      


Gln Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Ser Asn Gln Lys Asn Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Val Tyr Phe Thr Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ile Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln 
                85                  90                  95      


His Tyr Ser Ile Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


<210>  26
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  AAW69164

<400>  26

Asp Val Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Asn Thr 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Val Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Leu Ala Ser Thr Arg Glu Phe Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr His Ser Ser Pro His Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  27
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  CAI99839

<400>  27

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Asn 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Asn Leu Val Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Leu Gln 
                85                  90                  95      


Tyr Tyr Ser Thr Pro Leu Thr Phe Gly Gln Gly Thr Gln Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  28
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  AMK70392

<400>  28

Asp Ile Gln Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Lys Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Arg Ala Ser Thr Arg Asp Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Thr Pro Gln Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  29
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  ALV87854

<400>  29

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Arg 
            20                  25                  30          


Ser Lys Asn Lys Asn Tyr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Val Leu Ile Tyr Ser Thr Ser Thr Arg Ala Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Leu Gln 
                85                  90                  95      


Tyr Tyr Ile Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  30
<211>  113
<212>  PRT
<213>  artificial

<220>
<223>  IGKV4-1

<400>  30

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  31
<211>  23
<212>  PRT
<213>  artificial

<220>
<223>  primer CD47 sense

<400>  31

Ala Gly Ala Ala Gly Gly Thr Gly Ala Ala Ala Cys Gly Ala Thr Cys 
1               5                   10                  15      


Ala Thr Cys Gly Ala Gly Cys 
            20              


<210>  32
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  primer CD47 antisense

<400>  32

Cys Thr Cys Ala Thr Cys Cys Ala Thr Ala Cys Cys Ala Cys Cys Gly 
1               5                   10                  15      


Gly Ala Thr Cys Thr 
            20      


<210>  33
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  primer PD-L1 sense

<400>  33

Thr Gly Gly Cys Ala Thr Thr Thr Gly Cys Thr Gly Ala Ala Cys Gly 
1               5                   10                  15      


Cys Ala Thr Thr Thr 
            20      


<210>  34
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  primer PD-L1 antisense

<400>  34

Ala Gly Thr Gly Cys Ala Gly Cys Cys Ala Gly Gly Thr Cys Thr Ala 
1               5                   10                  15      


Ala Thr Thr Gly Thr 
            20      


<210>  35
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  primer beta-actin sense

<400>  35

Cys Cys Ala Ala Cys Cys Gly Cys Gly Ala Gly Ala Ala Gly Ala Thr 
1               5                   10                  15      


Gly Ala Cys Cys 
            20  


<210>  36
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  primer beta-actin antisense

<400>  36

Gly Ala Thr Cys Thr Thr Cys Ala Thr Gly Ala Gly Gly Thr Ala Gly 
1               5                   10                  15      


Thr Cys Ala Gly Thr 
            20      


