                         SEQUENCE LISTING

<110>  Janssen Vaccines & Prevention B.V.
 
<120>  Protein Purification

<130>  CRU6034WOPCT1

<150>  US 62/932,180
<151>  2019-11-07

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  679
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIV clade C gp140 protein

<400>  1

Ala Glu Asn Leu Trp Val Gly Asn Met Trp Val Thr Val Tyr Tyr Gly 
1               5                   10                  15      


Val Pro Val Trp Thr Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp 
            20                  25                  30          


Thr Lys Ala Tyr Asp Arg Glu Val His Asn Val Trp Ala Thr His Ala 
        35                  40                  45              


Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Ile Val Leu Glu Asn Val 
    50                  55                  60                  


Thr Glu Asn Phe Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His 
65                  70                  75                  80  


Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys 
                85                  90                  95      


Leu Thr Pro Leu Cys Val Thr Leu His Cys Thr Asn Ala Thr Phe Lys 
            100                 105                 110         


Asn Asn Val Thr Asn Asp Met Asn Lys Glu Ile Arg Asn Cys Ser Phe 
        115                 120                 125             


Asn Thr Thr Thr Glu Ile Arg Asp Lys Lys Gln Gln Gly Tyr Ala Leu 
    130                 135                 140                 


Phe Tyr Arg Pro Asp Ile Val Leu Leu Lys Glu Asn Arg Asn Asn Ser 
145                 150                 155                 160 


Asn Asn Ser Glu Tyr Ile Leu Ile Asn Cys Asn Ala Ser Thr Ile Thr 
                165                 170                 175     


Gln Ala Cys Pro Lys Val Asn Phe Asp Pro Ile Pro Ile His Tyr Cys 
            180                 185                 190         


Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr Phe Ser 
        195                 200                 205             


Gly Lys Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr His Gly 
    210                 215                 220                 


Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
225                 230                 235                 240 


Glu Lys Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn Val Lys 
                245                 250                 255     


Thr Ile Ile Val His Leu Asn Lys Ser Val Glu Ile Val Cys Thr Arg 
            260                 265                 270         


Pro Asn Asn Asn Thr Arg Lys Ser Met Arg Ile Gly Pro Gly Gln Thr 
        275                 280                 285             


Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala Tyr Cys 
    290                 295                 300                 


Asn Ile Ser Gly Ser Lys Trp Asn Glu Thr Leu Lys Arg Val Lys Glu 
305                 310                 315                 320 


Lys Leu Gln Glu Asn Tyr Asn Asn Asn Lys Thr Ile Lys Phe Ala Pro 
                325                 330                 335     


Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys Arg 
            340                 345                 350         


Gly Glu Phe Phe Tyr Cys Asn Thr Thr Arg Leu Phe Asn Asn Asn Ala 
        355                 360                 365             


Thr Glu Asp Glu Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile 
    370                 375                 380                 


Asn Met Trp Gln Gly Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala 
385                 390                 395                 400 


Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Val Arg 
                405                 410                 415     


Asp Gly Gly Glu Asp Asn Lys Thr Glu Glu Ile Phe Arg Pro Gly Gly 
            420                 425                 430         


Gly Asn Met Lys Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val 
        435                 440                 445             


Ile Glu Leu Lys Pro Leu Gly Ile Ala Pro Thr Gly Ala Lys Glu Arg 
    450                 455                 460                 


Val Val Glu Arg Glu Glu Arg Ala Val Gly Ile Gly Ala Val Phe Leu 
465                 470                 475                 480 


Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Leu Thr 
                485                 490                 495     


Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Ser Ile Val Gln Gln Gln 
            500                 505                 510         


Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Met Leu Gln Leu 
        515                 520                 525             


Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile Glu 
    530                 535                 540                 


Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly 
545                 550                 555                 560 


Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn 
                565                 570                 575     


Lys Ser Gln Thr Asp Ile Trp Asn Asn Met Thr Trp Met Glu Trp Asp 
            580                 585                 590         


Arg Glu Ile Ser Asn Tyr Thr Asp Thr Ile Tyr Arg Leu Leu Glu Asp 
        595                 600                 605             


Ser Gln Thr Gln Gln Glu Lys Asn Glu Lys Asp Leu Leu Ala Leu Asp 
    610                 615                 620                 


Ser Trp Lys Asn Leu Trp Ser Trp Phe Asp Ile Ser Asn Trp Leu Trp 
625                 630                 635                 640 


Tyr Ile Lys Ser Arg Ile Glu Gly Arg Gly Ser Gly Gly Tyr Ile Pro 
                645                 650                 655     


Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp 
            660                 665                 670         


Val Leu Leu Ser Thr Phe Leu 
        675                 


<210>  2
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIV mosaic gp140 protein

<400>  2

Ala Gly Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp 
            20                  25                  30          


Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Val Val Leu Glu Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Thr Asp Asp Val Arg Asn Val Thr Asn Asn Ala 
            100                 105                 110         


Thr Asn Thr Asn Ser Ser Trp Gly Glu Pro Met Glu Lys Gly Glu Ile 
        115                 120                 125             


Lys Asn Cys Ser Phe Asn Ile Thr Thr Ser Ile Arg Asn Lys Val Gln 
    130                 135                 140                 


Lys Gln Tyr Ala Leu Phe Tyr Lys Leu Asp Val Val Pro Ile Asp Asn 
145                 150                 155                 160 


Asp Ser Asn Asn Thr Asn Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val 
                165                 170                 175     


Ile Thr Gln Ala Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His 
            180                 185                 190         


Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu Lys Cys Asn Asp Lys Lys 
        195                 200                 205             


Phe Asn Gly Thr Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr 
    210                 215                 220                 


His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser 
225                 230                 235                 240 


Leu Ala Glu Glu Glu Val Val Ile Arg Ser Glu Asn Phe Thr Asn Asn 
                245                 250                 255     


Ala Lys Thr Ile Met Val Gln Leu Asn Val Ser Val Glu Ile Asn Cys 
            260                 265                 270         


Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly 
        275                 280                 285             


Arg Ala Phe Tyr Thr Ala Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala 
    290                 295                 300                 


His Cys Asn Ile Ser Arg Ala Asn Trp Asn Asn Thr Leu Arg Gln Ile 
305                 310                 315                 320 


Val Glu Lys Leu Gly Lys Gln Phe Gly Asn Asn Lys Thr Ile Val Phe 
                325                 330                 335     


Asn His Ser Ser Gly Gly Asp Pro Glu Ile Val Met His Ser Phe Asn 
            340                 345                 350         


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Lys Leu Phe Asn Ser 
        355                 360                 365             


Thr Trp Thr Trp Asn Asn Ser Thr Trp Asn Asn Thr Lys Arg Ser Asn 
    370                 375                 380                 


Asp Thr Glu Glu His Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile 
385                 390                 395                 400 


Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Arg 
                405                 410                 415     


Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg 
            420                 425                 430         


Asp Gly Gly Asn Asp Thr Ser Gly Thr Glu Ile Phe Arg Pro Gly Gly 
        435                 440                 445             


Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val 
    450                 455                 460                 


Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Glu Arg 
465                 470                 475                 480 


Val Val Gln Arg Glu Glu Arg Ala Val Gly Ile Gly Ala Val Phe Leu 
                485                 490                 495     


Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr 
            500                 505                 510         


Leu Thr Val Gln Ala Arg Leu Leu Leu Ser Gly Ile Val Gln Gln Gln 
        515                 520                 525             


Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu 
    530                 535                 540                 


Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu 
545                 550                 555                 560 


Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly 
                565                 570                 575     


Lys Leu Ile Cys Thr Thr Thr Val Pro Trp Asn Ala Ser Trp Ser Asn 
            580                 585                 590         


Lys Ser Leu Asp Lys Ile Trp Asn Asn Met Thr Trp Met Glu Trp Glu 
        595                 600                 605             


Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile Tyr Thr Leu Ile Glu Glu 
    610                 615                 620                 


Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp 
625                 630                 635                 640 


Lys Trp Ala Ser Leu Trp Asn Trp Phe Asp Ile Ser Asn Trp Leu Trp 
                645                 650                 655     


Tyr Ile Lys Ser Arg Ile Glu Gly Arg Gly Ser Gly Gly Tyr Ile Pro 
            660                 665                 670         


Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp 
        675                 680                 685             


Val Leu Leu Ser Thr Phe Leu 
    690                 695 


<210>  3
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIV leader/signal sequence

<400>  3

Met Arg Val Arg Gly Ile Gln Arg Asn Cys Gln His Leu Trp Arg Trp 
1               5                   10                  15      


Gly Thr Leu Ile Leu Gly Met Leu Met Ile Cys Ser Ala 
            20                  25                  


