                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Stabilized liquid enzyme compositions for brewing

<130>  15087-WO-PCT

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  622
<212>  PRT
<213>  Aspergillus niger

<400>  1

Asn Val Ile Ser Lys Arg Ala Thr Leu Asp Ser Trp Leu Ser Asn Glu 
1               5                   10                  15      


Ala Thr Val Ala Arg Thr Ala Ile Leu Asn Asn Ile Gly Ala Asp Gly 
            20                  25                  30          


Ala Trp Val Ser Gly Ala Asp Ser Gly Ile Val Val Ala Ser Pro Ser 
        35                  40                  45              


Thr Asp Asn Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ser Gly Leu 
    50                  55                  60                  


Val Leu Lys Thr Leu Val Asp Leu Phe Arg Asn Gly Asp Thr Ser Leu 
65                  70                  75                  80  


Leu Ser Thr Ile Glu Asn Tyr Ile Ser Ala Gln Ala Ile Val Gln Gly 
                85                  90                  95      


Ile Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Ala Gly Leu Gly Glu 
            100                 105                 110         


Pro Lys Phe Asn Val Asp Glu Thr Ala Tyr Thr Gly Ser Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Gly Phe 
    130                 135                 140                 


Gly Gln Trp Leu Leu Asp Asn Gly Tyr Thr Ser Thr Ala Thr Asp Ile 
145                 150                 155                 160 


Val Trp Pro Leu Val Arg Asn Asp Leu Ser Tyr Val Ala Gln Tyr Trp 
                165                 170                 175     


Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser Ser Phe 
            180                 185                 190         


Phe Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser Ala Phe 
        195                 200                 205             


Ala Thr Ala Val Gly Ser Ser Cys Ser Trp Cys Asp Ser Gln Ala Pro 
    210                 215                 220                 


Glu Ile Leu Cys Tyr Leu Gln Ser Phe Trp Thr Gly Ser Phe Ile Leu 
225                 230                 235                 240 


Ala Asn Phe Asp Ser Ser Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu 
                245                 250                 255     


Gly Ser Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp Ser Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Glu Val Val 
        275                 280                 285             


Asp Ser Phe Arg Ser Ile Tyr Thr Leu Asn Asp Gly Leu Ser Asp Ser 
    290                 295                 300                 


Glu Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Thr Tyr Tyr Asn Gly 
305                 310                 315                 320 


Asn Pro Trp Phe Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Tyr Gln Trp Asp Lys Gln Gly Ser Leu Glu Val Thr Asp Val 
            340                 345                 350         


Ser Leu Asp Phe Phe Lys Ala Leu Tyr Ser Asp Ala Ala Thr Gly Thr 
        355                 360                 365             


Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Ser Ile Val Asp Ala Val Lys 
    370                 375                 380                 


Thr Phe Ala Asp Gly Phe Val Ser Ile Val Glu Thr His Ala Ala Ser 
385                 390                 395                 400 


Asn Gly Ser Met Ser Glu Gln Tyr Asp Lys Ser Asp Gly Glu Gln Leu 
                405                 410                 415     


Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr Ala Asn 
            420                 425                 430         


Asn Arg Arg Asn Ser Val Val Pro Ala Ser Trp Gly Glu Thr Ser Ala 
        435                 440                 445             


Ser Ser Val Pro Gly Thr Cys Ala Ala Thr Ser Ala Ile Gly Thr Tyr 
    450                 455                 460                 


Ser Ser Val Thr Val Thr Ser Trp Pro Ser Ile Val Ala Thr Gly Gly 
465                 470                 475                 480 


Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val Thr Ser Thr 
                485                 490                 495     


Ser Lys Thr Thr Ala Thr Ala Ser Lys Thr Ser Thr Ser Thr Ser Ser 
            500                 505                 510         


Thr Ser Cys Thr Thr Pro Thr Ala Val Ala Val Thr Phe Asp Leu Thr 
        515                 520                 525             


Ala Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser Ile Ser 
    530                 535                 540                 


Gln Leu Gly Asp Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser Ala Asp 
545                 550                 555                 560 


Lys Tyr Thr Ser Ser Asp Pro Leu Trp Tyr Val Thr Val Thr Leu Pro 
                565                 570                 575     


Ala Gly Glu Ser Phe Glu Tyr Lys Phe Ile Arg Ile Glu Ser Asp Asp 
            580                 585                 590         


Ser Val Glu Trp Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val Pro Gln 
        595                 600                 605             


Ala Cys Gly Thr Ser Thr Ala Thr Val Thr Asp Thr Trp Arg 
    610                 615                 620         


<210>  2
<211>  591
<212>  PRT
<213>  Talaromyces emersonii

<400>  2

Ala Thr Gly Ser Leu Asp Ser Phe Leu Ala Thr Glu Thr Pro Ile Ala 
1               5                   10                  15      


Leu Gln Gly Val Leu Asn Asn Ile Gly Pro Asn Gly Ala Asp Val Ala 
            20                  25                  30          


Gly Ala Ser Ala Gly Ile Val Val Ala Ser Pro Ser Arg Ser Asp Pro 
        35                  40                  45              


Asn Tyr Phe Tyr Ser Trp Thr Arg Asp Ala Ala Leu Thr Ala Lys Tyr 
    50                  55                  60                  


Leu Val Asp Ala Phe Ile Ala Gly Asn Lys Asp Leu Glu Gln Thr Ile 
65                  70                  75                  80  


Gln Gln Tyr Ile Ser Ala Gln Ala Lys Val Gln Thr Ile Ser Asn Pro 
                85                  90                  95      


Ser Gly Asp Leu Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn Val 
            100                 105                 110         


Asn Glu Thr Ala Phe Thr Gly Pro Trp Gly Arg Pro Gln Arg Asp Gly 
        115                 120                 125             


Pro Ala Leu Arg Ala Thr Ala Leu Ile Ala Tyr Ala Asn Tyr Leu Ile 
    130                 135                 140                 


Asp Asn Gly Glu Ala Ser Thr Ala Asp Glu Ile Ile Trp Pro Ile Val 
145                 150                 155                 160 


Gln Asn Asp Leu Ser Tyr Ile Thr Gln Tyr Trp Asn Ser Ser Thr Phe 
                165                 170                 175     


Asp Leu Trp Glu Glu Val Glu Gly Ser Ser Phe Phe Thr Thr Ala Val 
            180                 185                 190         


Gln His Arg Ala Leu Val Glu Gly Asn Ala Leu Ala Thr Arg Leu Asn 
        195                 200                 205             


His Thr Cys Ser Asn Cys Val Ser Gln Ala Pro Gln Val Leu Cys Phe 
    210                 215                 220                 


Leu Gln Ser Tyr Trp Thr Gly Ser Tyr Val Leu Ala Asn Phe Gly Gly 
225                 230                 235                 240 


Ser Gly Arg Ser Gly Lys Asp Val Asn Ser Ile Leu Gly Ser Ile His 
                245                 250                 255     


Thr Phe Asp Pro Ala Gly Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys 
            260                 265                 270         


Ser Ala Arg Ala Leu Ala Asn His Lys Val Val Thr Asp Ser Phe Arg 
        275                 280                 285             


Ser Ile Tyr Ala Ile Asn Ser Gly Ile Ala Glu Gly Ser Ala Val Ala 
    290                 295                 300                 


Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp Tyr 
305                 310                 315                 320 


Leu Ala Thr Ala Ala Ala Ala Glu Gln Leu Tyr Asp Ala Ile Tyr Gln 
                325                 330                 335     


Trp Lys Lys Ile Gly Ser Ile Ser Ile Thr Asp Val Ser Leu Pro Phe 
            340                 345                 350         


Phe Gln Asp Ile Tyr Pro Ser Ala Ala Val Gly Thr Tyr Asn Ser Gly 
        355                 360                 365             


Ser Thr Thr Phe Asn Asp Ile Ile Ser Ala Val Gln Thr Tyr Gly Asp 
    370                 375                 380                 


Gly Tyr Leu Ser Ile Val Glu Lys Tyr Thr Pro Ser Asp Gly Ser Leu 
385                 390                 395                 400 


Thr Glu Gln Phe Ser Arg Thr Asp Gly Thr Pro Leu Ser Ala Ser Ala 
                405                 410                 415     


Leu Thr Trp Ser Tyr Ala Ser Leu Leu Thr Ala Ser Ala Arg Arg Gln 
            420                 425                 430         


Ser Val Val Pro Ala Ser Trp Gly Glu Ser Ser Ala Ser Ser Val Pro 
        435                 440                 445             


Ala Val Cys Ser Ala Thr Ser Ala Thr Gly Pro Tyr Ser Thr Ala Thr 
    450                 455                 460                 


Asn Thr Val Trp Pro Ser Ser Gly Ser Gly Ser Ser Thr Thr Thr Ser 
465                 470                 475                 480 


Ser Ala Pro Cys Thr Thr Pro Thr Ser Val Ala Val Thr Phe Asp Glu 
                485                 490                 495     


Ile Val Ser Thr Ser Tyr Gly Glu Thr Ile Tyr Leu Ala Gly Ser Ile 
            500                 505                 510         


Pro Glu Leu Gly Asn Trp Ser Thr Ala Ser Ala Ile Pro Leu Arg Ala 
        515                 520                 525             


Asp Ala Tyr Thr Asn Ser Asn Pro Leu Trp Tyr Val Thr Val Asn Leu 
    530                 535                 540                 


Pro Pro Gly Thr Ser Phe Glu Tyr Lys Phe Phe Lys Asn Gln Thr Asp 
545                 550                 555                 560 


Gly Thr Ile Val Trp Glu Asp Asp Pro Asn Arg Ser Tyr Thr Val Pro 
                565                 570                 575     


Ala Tyr Cys Gly Gln Thr Thr Ala Ile Leu Asp Asp Ser Trp Gln 
            580                 585                 590     


<210>  3
<211>  828
<212>  PRT
<213>  Artificial

<220>
<223>  Pullulanase derived from Bacillus deramificans

<400>  3

Asp Ser Thr Ser Thr Glu Val Ile Val His Tyr His Arg Phe Asp Ser 
1               5                   10                  15      


Asn Tyr Ala Asn Trp Asp Leu Trp Met Trp Pro Tyr Gln Pro Val Asn 
            20                  25                  30          


Gly Asn Gly Ala Ala Tyr Glu Phe Ser Gly Lys Asp Asp Phe Gly Val 
        35                  40                  45              


Lys Ala Asp Val Gln Val Pro Gly Asp Asp Thr Gln Val Gly Leu Ile 
    50                  55                  60                  


Val Arg Thr Asn Asp Trp Ser Gln Lys Asn Thr Ser Asp Asp Leu His 
65                  70                  75                  80  


Ile Asp Leu Thr Lys Gly His Glu Ile Trp Ile Val Gln Gly Asp Pro 
                85                  90                  95      


Asn Ile Tyr Tyr Asn Leu Ser Asp Ala Gln Ala Ala Ala Thr Pro Lys 
            100                 105                 110         


Val Ser Asn Ala Tyr Leu Asp Asn Glu Lys Thr Val Leu Ala Lys Leu 
        115                 120                 125             


Thr Asn Pro Met Thr Leu Ser Asp Gly Ser Ser Gly Phe Thr Val Thr 
    130                 135                 140                 


Asp Lys Thr Thr Gly Glu Gln Ile Pro Val Thr Ala Ala Thr Asn Ala 
145                 150                 155                 160 


Asn Ser Ala Ser Ser Ser Glu Gln Thr Asp Leu Val Gln Leu Thr Leu 
                165                 170                 175     


Ala Ser Ala Pro Asp Val Ser His Thr Ile Gln Val Gly Ala Ala Gly 
            180                 185                 190         


Tyr Glu Ala Val Asn Leu Ile Pro Arg Asn Val Leu Asp Ser Ser Gln 
        195                 200                 205             


Tyr Tyr Tyr Ser Gly Asp Asp Leu Gly Asn Thr Tyr Thr His Lys Ala 
    210                 215                 220                 


Thr Thr Phe Lys Val Trp Ala Pro Thr Ser Thr Gln Val Asn Val Leu 
225                 230                 235                 240 


Leu Tyr Asn Ser Ala Thr Gly Ser Val Thr Lys Thr Val Pro Met Thr 
                245                 250                 255     


Ala Ser Gly His Gly Val Trp Glu Ala Thr Val Asn Gln Asn Leu Glu 
            260                 265                 270         


Asn Trp Tyr Tyr Met Tyr Glu Val Thr Gly Gln Gly Ser Thr Arg Thr 
        275                 280                 285             


Ala Val Asp Pro Tyr Ala Thr Ala Ile Ala Pro Asn Gly Thr Arg Gly 
    290                 295                 300                 


Met Ile Val Asp Leu Ala Lys Thr Asp Pro Ala Gly Trp Asn Ser Asp 
305                 310                 315                 320 


Lys His Ile Thr Pro Lys Asn Ile Glu Asp Glu Val Ile Tyr Glu Met 
                325                 330                 335     


Asp Val Arg Asp Phe Ser Ile Asp Pro Asn Ser Gly Met Lys Asn Lys 
            340                 345                 350         


Gly Lys Tyr Leu Ala Leu Thr Glu Lys Gly Thr Lys Gly Pro Asp Gly 
        355                 360                 365             


Val Lys Thr Gly Ile Asp Ser Leu Lys Gln Leu Gly Ile Thr His Val 
    370                 375                 380                 


Gln Leu Met Pro Val Phe Ala Phe Ala Ser Val Asp Glu Thr Asp Pro 
385                 390                 395                 400 


Thr Gln Asp Asn Trp Gly Tyr Asp Pro Arg Asn Tyr Asp Val Pro Glu 
                405                 410                 415     


Gly Gln Tyr Ala Thr Asn Ala Asn Gly Thr Ala Arg Ile Lys Glu Phe 
            420                 425                 430         


Lys Glu Met Val Leu Ser Leu His Arg Glu His Ile Gly Val Asn Met 
        435                 440                 445             


Asp Val Val Tyr Asn His Thr Phe Ala Thr Gln Ile Ser Asp Phe Asp 
    450                 455                 460                 


Lys Ile Val Pro Glu Tyr Tyr Tyr Arg Thr Asp Asp Ala Gly Asn Tyr 
465                 470                 475                 480 


Thr Asn Gly Ser Gly Thr Gly Asn Glu Ile Ala Ser Glu Arg Pro Met 
                485                 490                 495     


Val Gln Lys Phe Ile Ile Asp Ser Leu Lys Tyr Trp Val Asn Glu Tyr 
            500                 505                 510         


His Ile Asp Gly Phe Arg Phe Asp Leu Met Ala Leu Leu Gly Lys Asp 
        515                 520                 525             


Thr Met Ser Lys Ala Ala Ser Glu Leu His Ala Ile Asn Pro Gly Ile 
    530                 535                 540                 


Ala Leu Tyr Gly Glu Pro Trp Thr Gly Gly Thr Ser Ala Leu Pro Glu 
545                 550                 555                 560 


Asp Gln Leu Leu Thr Lys Gly Ala Gln Lys Gly Met Gly Val Ala Val 
                565                 570                 575     


Phe Asn Asp Asn Leu Arg Asn Ala Leu Asp Gly Asn Val Phe Asp Ser 
            580                 585                 590         


Ser Ala Gln Gly Phe Ala Thr Gly Ala Thr Gly Leu Thr Asp Ala Ile 
        595                 600                 605             


Lys Asn Gly Val Glu Gly Ser Ile Asn Asp Phe Thr Ser Ser Pro Gly 
    610                 615                 620                 


Glu Thr Ile Asn Tyr Val Thr Ser His Asp Asn Tyr Thr Leu Trp Asp 
625                 630                 635                 640 


Lys Ile Ala Leu Ser Asn Pro Asn Asp Ser Glu Ala Asp Arg Ile Lys 
                645                 650                 655     


Met Asp Glu Leu Ala Gln Ala Val Val Met Thr Ser Gln Gly Val Pro 
            660                 665                 670         


Phe Met Gln Gly Gly Glu Glu Met Leu Arg Thr Lys Gly Gly Asn Asp 
        675                 680                 685             


Asn Ser Tyr Asn Ala Gly Asp Thr Val Asn Glu Phe Asp Trp Ser Arg 
    690                 695                 700                 


Lys Ala Gln Tyr Pro Asp Val Phe Asn Tyr Tyr Ser Gly Leu Ile His 
705                 710                 715                 720 


Leu Arg Leu Asp His Pro Ala Phe Arg Met Thr Thr Ala Asn Glu Ile 
                725                 730                 735     


Asn Ser His Leu Gln Phe Leu Asn Ser Pro Glu Asn Thr Val Ala Tyr 
            740                 745                 750         


Glu Leu Thr Asp His Val Asn Lys Asp Lys Trp Gly Asn Ile Ile Val 
        755                 760                 765             


Val Tyr Asn Pro Asn Lys Thr Ala Ala Thr Ile Asn Leu Pro Ser Gly 
    770                 775                 780                 


Lys Trp Ala Ile Asn Ala Thr Ser Gly Lys Val Gly Glu Ser Thr Leu 
785                 790                 795                 800 


Gly Gln Ala Glu Gly Ser Val Gln Val Pro Gly Ile Ser Met Met Ile 
                805                 810                 815     


Leu His Gln Glu Val Ser Pro Asp His Gly Lys Lys 
            820                 825             


