                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  LOW VOLUME TRANSFECTION

<130>  33478-WO-PCT

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the protospacer used to target the CRISPR-Cas9 
       complex to fwnA (An09g05730)

<400>  1
tcagatatat tcagtcactg                                                   20


<210>  2
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the DNA fragment consisting of two 50bp parts 
       homologous to the sequences flanking the fwnA (An09g05730) open 
       reading frame

<400>  2
ttgcatcgct tgcgttcatg atgaacccag acaggtgtcc aaaactgtca gactgtagct       60

gttgctccat cacttcgagt ctcaatgacc ttctgagccg                            100


<210>  3
<211>  11040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pAMA-hygB

<400>  3
ggtaccacgc tttccggcat cttccagacc acagtatatc catccgcctc ctgttgaagc       60

ttattttttg tatactgttt tgtgatagca cgaagttttt ccacggtatc ttgttaaaaa      120

tatatatttg tggcgggctt acctacatca aattaataag agactaatta taaactaaac      180

acacaagcaa gctactttag ggtaaaagtt tataaatgct tttgacgtat aaacgttgct      240

tgtatttatt attacaatta aaggtggata gaaaacctag agactagtta gaaactaatc      300

tcaggtttgc gttaaactaa atcagagccc gagaggttaa cagaacctag aaggggacta      360

gatatccggg tagggaaaca aaaaaaaaaa acaagacagc cacatattag ggagactagt      420

tagaagctag ttccaggact aggaaaataa aagacaatga taccacagtc tagttgacaa      480

ctagatagat tctagattga ggccaaagtc tctgagatcc aggttagttg caactaatac      540

tagttagtat ctagtctcct ataactctga agctagaata acttactact attatcctca      600

ccactgttca gctgcgcaaa cggagtgatt gcaaggtgtt cagagactag ttattgacta      660

gtcagtgact agcaataact aacaaggtat taacctacca tgtctgccat caccctgcac      720

ttcctcgggc tcagcagcct tttcctcctc attttcatgc tcattttcct tgtttaagac      780

tgtgactagt caaagactag tccagaacca caaaggagaa atgtcttacc actttcttca      840

ttgcttgtct cttttgcatt atccatgtct gcaactagtt agagtctagt tagtgactag      900

tccgacgagg acttgcttgt ctccggattg ttggaggaac tctccagggc ctcaagatcc      960

acaacagagc cttctagaag actggtcaat aactagttgg tctttgtctg agtctgactt     1020

acgaggttgc atactcgctc cctttgcctc gtcaatcgat gagaaaaagc gccaaaactc     1080

gcaatatggc tttgaaccac acggtgctga gactagttag aatctagtcc caaactagct     1140

tggatagctt acctttgccc tttgcgttgc gacaggtctt gcagggtatg gttcctttct     1200

caccagctga tttagctgcc ttgctaccct cacggcggat ctgcataaag agtggctaga     1260

ggttataaat tagcactgat cctaggtacg gggctgaatg taacttgcct ttcctttctc     1320

atcgcgcggc aagacaggct tgctcaaatt cctaccagtc acaggggtat gcacggcgta     1380

cggaccactt gaactagtca cagattagtt agcaactagt ctgcattgaa tggctgtact     1440

tacgggccct cgccattgtc ctgatcattt ccagcttcac cctcgttgct gcaaagtagt     1500

tagtgactag tcaaggacta gttgaaatgg gagaagaaac tcacgaattc tcgacaccct     1560

tagtattgtg gtccttggac ttggtgctgc tatatattag ctaatacact agttagactc     1620

acagaaactt acgcagctcg cttgcgcttc ttggtaggag tcggggttgg gagaacagtg     1680

ccttcaaaca agccttcata ccatgctact tgactagtca gggactagtc accaagtaat     1740

ctagatagga cttgcctttg gcctccatca gttccttcat agtgggaggt ccattgtgca     1800

atgtaaactc catgccgtgg gagttcttgt ccttcaagtg cttgaccaat atgtttctgt     1860

tggcagaggg aacctgtcaa ctagttaata actagtcaga aactagtata gcagtagact     1920

cactgtacgc ttgaggcatc ccttcactcg gcagtagact tcatatggat ggatatcagg     1980

cacgccattg tcgtcctgtg gactagtcag taactaggct taaagctagt cgggtcggct     2040

tactatcttg aaatccggca gcgtaagctc cccgtcctta actgcctcga gatagtgaca     2100

gtactctggg gactttcgga gatcgttatc gcgaatgctc ggcatactaa tcgttgacta     2160

gtcttggact agtcccgagc aaaaaggatt ggaggaggag gaggaaggtg agagtgagac     2220

aaagagcgaa ataagagctt caaaggctat ctctaagcag tatgaaggtt aagtatctag     2280

ttcttgacta gatttaaaag agatttcgac tagttatgta cctggagttt ggatatagga     2340

atgtgttgtg gtaacgaaat gtaaggggga ggaaagaaaa agtcggtcaa gaggtaactc     2400

taagtcggcc attccttttt gggaggcgct aaccataaac ggcatggtcg acttagagtt     2460

agctcaggga atttagggag ttatctgcga ccaccgagga acggcggaat gccaaagaat     2520

cccgatggag ctctagctgg cggttgacaa ccccaccttt tggcgtttct gcggcgttgc     2580

aggcgggact ggatacttcg tagaaccaga aaggcaaggc agaacgcgct cagcaagagt     2640

gttggaagtg atagcatgat gtgccttgtt aactaggtca aaatctgcag tatgcttgat     2700

gttatccaaa gtgtgagaga ggaaggtcca aacatacacg attgggagag ggcctaggta     2760

taagagtttt tgagtagaac gcatgtgagc ccagccatct cgaggagatt aaacacgggc     2820

cggcatttga tggctatgtt agtaccccaa tggaaagcct gagagtccag tggtcgcaga     2880

taactcccta aattccctga gctaactcta agtcgaccat gccgtttatg gttagcgcct     2940

cccaaaaagg aatggccgac ttagagttac ctcttgaccg actttttctt tcctccccct     3000

tacatttcgt taccacaaca cattcctata tccaaactcc aggtacataa ctagtcgaaa     3060

tctcttttaa atctagtcaa gaactagata cttaaccttc atactgctta gagatagcct     3120

ttgaagctct tatttcgctc tttgtctcac tctcaccttc ctcctcctcc tccaatcctt     3180

tttgctcggg actagtccaa gactagtcaa cgattagtat gccgagcatt cgcgataacg     3240

atctccgaaa gtccccagag tactgtcact atctcgaggc agttaaggac ggggagctta     3300

cgctgccgga tttcaagata gtaagccgac ccgactagct ttaagcctag ttactgacta     3360

gtccacagga cgacaatggc gtgcctgata tccatccata tgaagtctac tgccgagtga     3420

agggatgcct caagcgtaca gtgagtctac tgctatacta gtttctgact agttattaac     3480

tagttgacag gttccctctg ccaacagaaa catattggtc aagcacttga aggacaagaa     3540

ctcccacggc atggagttta cattgcacaa tggacctccc actatgaagg aactgatgga     3600

ggccaaaggc aagtcctatc tagattactt ggtgactagt ccctgactag tcaagtagca     3660

tggtatgaag gcttgtttga aggcactgtt ctcccaaccc cgactcctac caagaagcgc     3720

aagcgagctg cgtaagtttc tgtgagtcta actagtgtat tagctaatat atagcagcac     3780

caagtccaag gaccacaata ctaagggtgt cgagaattcg tgagtttctt ctcccatttc     3840

aactagtcct tgactagtca ctaactactt tgcagcaacg agggtgaagc tggaaatgat     3900

caggacaatg gcgagggccc gtaagtacag ccattcaatg cagactagtt gctaactaat     3960

ctgtgactag ttcaagtggt ccgtacgccg tgcatacccc tgtgactggt aggaatttga     4020

gcaagcctgt cttgccgcgc gatgagaaag gaaaggcaag ttacattcag ccccgtacct     4080

aggatcagtg ctaatttata acctctagcc actctttatg cagatccgcc gtgagggtag     4140

caaggcagct aaatcagctg gtgagaaagg aaccataccc tgcaagacct gtcgcaacgc     4200

aaagggcaaa ggtaagctat ccaagctagt ttgggactag attctaacta gtctcagcac     4260

cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt ctcatcgatt gacgaggcaa     4320

agggagcgag tatgcaacct cgtaagtcag actcagacaa agaccaacta gttattgacc     4380

agtcttctag aaggctctgt tgtggatctt gaggccctgg agagttcctc caacaatccg     4440

gagacaagca agtcctcgtc ggactagtca ctaactagac tctaactagt tgcagacatg     4500

gataatgcaa aagagacaag caatgaagaa agtggtaaga catttctcct ttgtggttct     4560

ggactagtct ttgactagtc acagtcttaa acaaggaaaa tgagcatgaa aatgaggagg     4620

aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag acatggtagg ttaatacctt     4680

gttagttatt gctagtcact gactagtcaa taactagtct ctgaacacct tgcaatcact     4740

ccgtttgcgc agctgaacag tggtgaggat aatagtagta agttattcta gcttcagagt     4800

tataggagac tagatactaa ctagtattag ttgcaactaa cctggatctc agagactttg     4860

gcctcaatct agaatctatc tagttgtcaa ctagactgtg gtatcattgt cttttatttt     4920

cctagtcctg gaactagctt ctaactagtc tccctaatat gtggctgtct tgtttttttt     4980

ttttgtttcc ctacccggat atctagtccc cttctaggtt ctgttaacct ctcgggctct     5040

gatttagttt aacgcaaacc tgagattagt ttctaactag tctctaggtt ttctatccac     5100

ctttaattgt aataataaat acaagcaacg tttatacgtc aaaagcattt ataaactttt     5160

accctaaagt agcttgcttg tgtgtttagt ttataattag tctcttatta atttgatgta     5220

ggtaagcccg ccacaaatat atatttttaa caagataccg tggaaaaact tcgtgctatc     5280

acaaaacagt atacaaaaaa taagctatcg aattcctgca gagatcatcc tgtcttcagt     5340

cttaagactt ctctcctata tcacccgcac ttaccctaga gtgccgctta ggtgctaagg     5400

gcacattgag tattggccgt gtagaatata tagcttaagt acggccaagc agacgggaag     5460

ccctgttctc cacaccctat ggtcgtatat atcaggcttc taccgggaaa cgattaagag     5520

tgtataatgg actgaaaatc aatatgaacg ggacaatgct caagttaaat tagttaggca     5580

tcctaatctc tactaaatgt tctatctaga gatcggggta ctataggccc gtacgttaat     5640

cactctacgc ttctctccct taggtatagt gtaggtaggg gctagacatt tatatgagtc     5700

agatggtaca aacggtaggc agtgcgggcg aagaagtgaa gacggagtcg gttgaagcta     5760

catacaaaag atgcattggc tcgtcatgaa gagcctcccg ggtttattcc tttgccctcg     5820

gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc     5880

agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga     5940

cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc     6000

tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg     6060

caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg     6120

gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc     6180

agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg     6240

cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag     6300

cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg     6360

gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc     6420

cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct     6480

ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac     6540

cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca     6600

cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga     6660

aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga     6720

aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt     6780

cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg cattttgacg gtgggatcct     6840

gtgatgtctg ctcaagcggg gtagctgtta gtcaagctgc gatgaagtgg gaaagctcga     6900

actgaaaggt tcaaaggaat aagggatggg aaggatggag tatggatgta gcaaagtact     6960

tacttagggg aaataaaggt tcttggatgg gaagatgaat atactgaaga tgggaaaaga     7020

aagagaaaag aaaagagcag ctggtgggga gagcaggaaa atatggcaac aaatgttgga     7080

ctgacgcaac gaccttgtca accccgccga cacaccgggc ggacagacgg ggcaaagctg     7140

cctaccaggg actgagggac ctcagcaggt cgagtgcaga gcaccggatg ggtcgactgc     7200

cagcttgtgt tcccggtctg cgccgctggc cagctcctga gcggcctttc cggtttcata     7260

caccgggcaa agcaggagag gcacgatatt tggacgccct acagatgccg gatgggccaa     7320

ttagggagct tacgcgccgg gtactcgctc tacctacttc ggagaaggta ctatctcgtg     7380

aatcttttac cagatcggaa gcaattggac ttctgtacct aggttaatgg catgctattt     7440

cgccgacggc tatacacccc tggcttcaca ttctccttcg cttactgccg gtgattcgat     7500

gaagctccat attctccgat gatgcaatag attcttggtc aacgaggggc acaccagcct     7560

ttccacttcg gggcggaggg gcggccggtc ccggattaat aatcatccac tgcacctcag     7620

agccgccaga gctgtctggc gcagtggcgc ttattactca gcccttctct ctgcgtccgt     7680

ccgtctctcc gcatgccaga aagagtcacc ggtcactgta cagagcggcc gccaccgcgg     7740

tggagctcca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta     7800

caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc     7860

cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg     7920

cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg     7980

gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct     8040

ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg     8100

ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag     8160

ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg     8220

gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc     8280

tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat     8340

gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag     8400

gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt     8460

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa     8520

ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt     8580

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt     8640

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt     8700

ttcgccccga agaacgtttt ccaatgatga gcacttttcg accgaataaa tacctgtgac     8760

ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg     8820

ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca     8880

taatgaaata agatcactac cgggcgtatt ttttgagttg tcgagatttt caggagctaa     8940

ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca     9000

tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt     9060

tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc     9120

ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat     9180

gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga     9240

gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct     9300

acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg     9360

gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga     9420

tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta     9480

tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga     9540

tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg     9600

cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg     9660

aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc     9720

ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg     9780

actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct     9840

ctccccgtgg aggtaataat tgacgatatg atcctttttt tctgatcaaa aaggatctag     9900

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     9960

tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc    10020

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat    10080

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat    10140

actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct    10200

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt    10260

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg    10320

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta    10380

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg    10440

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg    10500

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc    10560

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg    10620

gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat    10680

aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc    10740

agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg    10800

cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt    10860

gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt    10920

atgctcccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac    10980

agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg    11040


