﻿                         序列表

<110>  上海健康医学院

<120>  一种制备固定化精氨酸脱亚胺酶及生产[14/15N]-L-瓜氨酸的方法

<150>  2019110783706
<151>  2019-11-06

<160>  1

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1567
<212>  PRT
<213>  一种制备固定化精氨酸脱亚胺酶及生产[14/15N]-L-瓜氨酸的方法(Corynebacterium glutamicum)

<400>  1
Ser Glu Gln Glu Asn Cys Glu Leu Ile Ser Thr Ile Asn Gly Asn Leu 
1               5                   10                  15      
Pro Arg Thr Ala Thr Gly Ala Thr Cys Ala Ala Cys Gly Ala Cys Ala 
            20                  25                  30          
Thr Gly Cys Ala Cys Cys Cys Ala Thr Cys Cys Cys Thr Gly Ala Thr 
        35                  40                  45              
Cys Ala Ala Gly Gly Ala Cys Ala Ala Gly Gly Ala Cys Ala Thr Gly 
    50                  55                  60                  
Ala Thr Gly Gly Ala Cys Gly Ala Cys Gly Thr Thr Ala Thr Gly Cys 
65                  70                  75                  80  
Thr Gly Cys Gly Cys Thr Cys Cys Thr Gly Cys Ala Ala Gly Ala Thr 
                85                  90                  95      
Cys Ala Thr Cys Gly Cys Thr Ala Thr Gly Ala Ala Gly Ala Thr Cys 
            100                 105                 110         
Ala Thr Gly Cys Cys Ala Gly Ala Cys Ala Ala Gly Gly Thr Thr Ala 
        115                 120                 125             
Thr Gly Cys Ala Gly Gly Thr Thr Ala Thr Gly Gly Thr Thr Ala Cys 
    130                 135                 140                 
Cys Gly Thr Thr Cys Thr Gly Ala Thr Gly Cys Thr Gly Gly Ala Cys 
145                 150                 155                 160 
Gly Gly Cys Ala Cys Cys Thr Cys Cys Gly Ala Gly Gly Ala Gly Ala 
                165                 170                 175     
Thr Gly Cys Thr Gly Cys Thr Gly Ala Ala Gly Thr Gly Gly Ala Ala 
            180                 185                 190         
Cys Cys Thr Gly Cys Thr Gly Gly Ala Cys Ala Ala Cys Cys Gly Cys 
        195                 200                 205             
Gly Gly Cys Ala Thr Gly Gly Cys Thr Ala Thr Cys Thr Ala Cys Ala 
    210                 215                 220                 
Ala Gly Gly Thr Thr Cys Thr Gly Ala Thr Gly Gly Ala Gly Gly Cys 
225                 230                 235                 240 
Thr Cys Thr Gly Thr Gly Cys Gly Gly Cys Ala Ala Gly Ala Ala Gly 
                245                 250                 255     
Gly Ala Cys Gly Thr Thr Ala Ala Gly Ala Thr Cys Gly Gly Cys Ala 
            260                 265                 270         
Cys Cys Gly Thr Thr Gly Gly Cys Ala Ala Gly Gly Thr Thr Gly Gly 
        275                 280                 285             
Cys Cys Cys Ala Cys Thr Gly Gly Gly Cys Thr Gly Cys Gly Ala Cys 
    290                 295                 300                 
Thr Ala Cys Ala Thr Cys Ala Ala Cys Thr Gly Cys Gly Thr Thr Gly 
305                 310                 315                 320 
Ala Gly Ala Thr Cys Thr Cys Cys Ala Thr Gly Pro Arg Thr Ala Thr 
                325                 330                 335     
Gly Ala Ala Cys Ala Ala Cys Gly Gly Cys Ala Thr Cys Ala Ala Cys 
            340                 345                 350         
Gly Thr Thr Ala Ala Cys Thr Cys Cys Gly Ala Gly Ala Thr Cys Gly 
        355                 360                 365             
Gly Cys Ala Ala Gly Cys Thr Gly Ala Ala Gly Thr Cys Cys Gly Thr 
    370                 375                 380                 
Thr Cys Thr Gly Cys Thr Gly Cys Ala Cys Cys Gly Cys Cys Cys Ala 
385                 390                 395                 400 
Gly Gly Cys Gly Cys Thr Gly Ala Gly Gly Thr Thr Gly Ala Gly Ala 
                405                 410                 415     
Ala Cys Ala Thr Cys Ala Cys Cys Cys Cys Ala Gly Ala Cys Ala Cys 
            420                 425                 430         
Cys Ala Thr Gly Ala Ala Gly Cys Ala Gly Cys Thr Gly Cys Thr Gly 
        435                 440                 445             
Thr Thr Cys Gly Ala Cys Gly Ala Cys Ala Thr Cys Cys Cys Ala Thr 
    450                 455                 460                 
Ala Cys Cys Thr Gly Ala Ala Gly Ala Thr Cys Gly Cys Thr Cys Ala 
465                 470                 475                 480 
Gly Ala Ala Gly Gly Ala Gly Cys Ala Cys Gly Ala Cys Thr Thr Cys 
                485                 490                 495     
Thr Thr Cys Gly Cys Thr Cys Ala Gly Ala Cys Cys Cys Thr Gly Cys 
            500                 505                 510         
Gly Cys Gly Ala Cys Ala Ala Cys Gly Gly Cys Gly Cys Thr Gly Ala 
        515                 520                 525             
Gly Ala Cys Cys Gly Thr Thr Thr Ala Cys Ala Thr Cys Gly Ala Gly 
    530                 535                 540                 
Ala Ala Cys Cys Thr Gly Gly Cys Thr Ala Cys Cys Gly Ala Gly Gly 
545                 550                 555                 560 
Thr Thr Thr Thr Cys Gly Ala Gly Ala Ala Gly Thr Cys Cys Thr Cys 
                565                 570                 575     
Cys Gly Ala Gly Ala Cys Cys Ala Ala Gly Gly Ala Gly Gly Ala Gly 
            580                 585                 590         
Thr Thr Cys Cys Thr Gly Thr Cys Cys Cys Ala Cys Cys Thr Gly Cys 
        595                 600                 605             
Thr Gly Cys Ala Cys Gly Ala Gly Gly Cys Thr Gly Gly Cys Thr Ala 
    610                 615                 620                 
Cys Cys Gly Cys Cys Cys Ala Gly Gly Cys Cys Gly Cys Ala Cys Cys 
625                 630                 635                 640 
Thr Ala Cys Gly Ala Cys Gly Gly Cys Cys Thr Gly Ala Cys Cys Gly 
                645                 650                 655     
Ala Gly Thr Ala Cys Cys Thr Gly Ala Cys Cys Thr Cys Cys Ala Thr 
            660                 665                 670         
Gly Thr Cys Cys Ala Cys Cys Ala Ala Gly Gly Ala Cys Ala Thr Gly 
        675                 680                 685             
Gly Thr Thr Gly Ala Gly Ala Ala Gly Ala Thr Cys Thr Ala Cys Gly 
    690                 695                 700                 
Cys Thr Gly Gly Cys Gly Thr Thr Cys Gly Cys Ala Ala Gly Ala Ala 
705                 710                 715                 720 
Cys Gly Ala Gly Cys Thr Gly Gly Ala Cys Ala Thr Cys Ala Ala Gly 
                725                 730                 735     
Cys Gly Cys Ala Cys Cys Gly Cys Thr Cys Thr Gly Thr Cys Cys Gly 
            740                 745                 750         
Ala Cys Ala Thr Gly Gly Cys Thr Gly Gly Cys Thr Cys Cys Gly Ala 
        755                 760                 765             
Cys Gly Cys Thr Gly Ala Gly Ala Ala Cys Thr Ala Cys Thr Thr Cys 
    770                 775                 780                 
Thr Ala Cys Cys Thr Gly Ala Ala Cys Cys Cys Ala Cys Thr Gly Cys 
785                 790                 795                 800 
Cys Ala Ala Ala Cys Gly Cys Thr Thr Ala Cys Thr Thr Cys Ala Cys 
                805                 810                 815     
Cys Cys Gly Cys Gly Ala Cys Cys Cys Ala Cys Ala Gly Gly Cys Thr 
            820                 825                 830         
Thr Cys Cys Ala Thr Gly Gly Gly Cys Gly Thr Thr Gly Gly Cys Ala 
        835                 840                 845             
Thr Gly Ala Cys Cys Ala Thr Cys Ala Ala Cys Ala Ala Gly Ala Thr 
    850                 855                 860                 
Gly Ala Cys Cys Thr Thr Cys Cys Cys Ala Gly Cys Thr Cys Gly Cys 
865                 870                 875                 880 
Cys Ala Gly Cys Cys Ala Gly Ala Gly Thr Cys Cys Cys Thr Gly Ala 
                885                 890                 895     
Thr Cys Ala Cys Cys Gly Ala Gly Thr Ala Cys Gly Thr Thr Ala Thr 
            900                 905                 910         
Gly Gly Cys Thr Ala Ala Cys Cys Ala Cys Cys Cys Ala Cys Gly Cys 
        915                 920                 925             
Thr Thr Cys Ala Ala Gly Gly Ala Cys Ala Cys Cys Cys Cys Ala Ala 
    930                 935                 940                 
Thr Cys Thr Gly Gly Cys Gly Cys Gly Ala Cys Cys Gly Cys Ala Ala 
945                 950                 955                 960 
Cys Cys Ala Cys Ala Cys Cys Ala Cys Cys Cys Gly Cys Ala Thr Cys 
                965                 970                 975     
Gly Ala Gly Gly Gly Cys Gly Gly Cys Gly Ala Cys Gly Ala Gly Cys 
            980                 985                 990         
Thr Gly Ala Thr Cys Cys Thr Gly  Ala Ala Cys Ala Ala  Gly Ala Cys 
        995                 1000                 1005             
Cys Ala  Cys Cys Gly Thr Thr  Gly Cys Thr Ala Thr  Cys Gly Gly Cys 
    1010                 1015                 1020                 
Gly  Thr Thr Thr Cys Cys  Gly Ala Gly Cys Gly  Cys Ala Cys Cys Thr  
1025                 1030                 1035                 1040 
Cys Cys Thr Cys Cys  Ala Ala Gly Ala Cys  Cys Ala Thr Cys Cys  Ala 
                1045                 1050                 1055     
Gly Ala Ala Cys  Cys Thr Gly Gly Cys  Thr Ala Ala Gly Gly  Ala Gly 
            1060                 1065                 1070         
Cys Thr Gly  Thr Thr Cys Gly Cys  Thr Ala Ala Cys Cys  Cys Ala Cys 
        1075                 1080                 1085             
Thr Gly  Thr Cys Cys Ala Cys  Cys Thr Thr Cys Gly  Ala Cys Ala Cys 
    1090                 1095                 1100                 
Cys  Gly Thr Thr Cys Thr  Gly Gly Cys Thr Gly  Thr Thr Gly Ala Gly  
1105                 1110                 1115                 1120 
Ala Thr Cys Cys Cys  Ala Cys Ala Cys Ala  Ala Cys Cys Ala Cys  Gly 
                1125                 1130                 1135     
Cys Thr Ala Thr  Gly Ala Thr Gly Cys  Ala Cys Cys Thr Gly  Gly Ala 
            1140                 1145                 1150         
Cys Ala Cys  Cys Gly Thr Thr Thr  Thr Cys Ala Cys Cys  Ala Thr Gly 
        1155                 1160                 1165             
Ala Thr  Cys Ala Ala Cys Cys  Ala Cys Gly Ala Cys  Cys Ala Gly Thr 
    1170                 1175                 1180                 
Thr  Cys Ala Cys Cys Gly  Thr Thr Thr Thr Cys  Cys Cys Ala Gly Gly  
1185                 1190                 1195                 1200 
Cys Ala Thr Cys Ala  Thr Gly Gly Ala Cys  Gly Gly Cys Gly Cys  Thr 
                1205                 1210                 1215     
Gly Gly Cys Ala  Ala Cys Ala Thr Cys  Ala Ala Cys Gly Thr  Thr Thr 
            1220                 1225                 1230         
Thr Cys Ala  Thr Cys Cys Thr Gly  Cys Gly Cys Cys Cys  Ala Gly Gly 
        1235                 1240                 1245             
Cys Ala  Ala Gly Gly Ala Cys  Gly Ala Cys Gly Ala  Gly Gly Thr Thr 
    1250                 1255                 1260                 
Gly  Ala Gly Ala Thr Cys  Gly Ala Gly Cys Ala  Cys Cys Thr Gly Ala  
1265                 1270                 1275                 1280 
Cys Cys Gly Ala Cys  Cys Thr Gly Ala Ala  Gly Gly Cys Thr Gly  Cys 
                1285                 1290                 1295     
Thr Cys Thr Gly  Ala Ala Gly Ala Ala  Gly Gly Thr Thr Cys  Thr Gly 
            1300                 1305                 1310         
Ala Ala Cys  Cys Thr Gly Thr Cys  Cys Gly Ala Gly Cys  Thr Gly Gly 
        1315                 1320                 1325             
Ala Cys  Cys Thr Gly Ala Thr  Cys Gly Ala Gly Thr  Gly Cys Gly Gly 
    1330                 1335                 1340                 
Cys  Gly Cys Thr Gly Gly  Cys Gly Ala Cys Cys  Cys Ala Ala Thr Cys  
1345                 1350                 1355                 1360 
Gly Cys Thr Gly Cys  Thr Cys Cys Ala Cys  Gly Cys Gly Ala Gly  Cys 
                1365                 1370                 1375     
Ala Gly Thr Gly  Gly Ala Ala Cys Gly  Ala Cys Gly Gly Cys  Thr Cys 
            1380                 1385                 1390         
Cys Ala Ala  Cys Ala Cys Cys Cys  Thr Gly Gly Cys Thr  Ala Thr Cys 
        1395                 1400                 1405             
Gly Cys  Thr Cys Cys Ala Gly  Gly Cys Gly Ala Gly  Ala Thr Cys Gly 
    1410                 1415                 1420                 
Thr  Thr Ala Cys Cys Thr  Ala Cys Gly Ala Cys  Cys Gly Cys Ala Ala  
1425                 1430                 1435                 1440 
Cys Thr Ala Cys Gly  Thr Thr Ala Cys Cys  Gly Thr Thr Gly Ala  Gly 
                1445                 1450                 1455     
Cys Thr Gly Cys  Thr Gly Ala Ala Gly  Gly Ala Gly Cys Ala  Cys Gly 
            1460                 1465                 1470         
Gly Cys Ala  Thr Cys Ala Ala Gly  Gly Thr Thr Cys Ala  Cys Gly Ala 
        1475                 1480                 1485             
Gly Ala  Thr Cys Cys Thr Gly  Thr Cys Cys Thr Cys  Cys Gly Ala Gly 
    1490                 1495                 1500                 
Cys  Thr Gly Gly Gly Cys  Cys Gly Cys Gly Gly  Cys Cys Gly Cys Gly  
1505                 1510                 1515                 1520 
Gly Cys Gly Gly Cys  Gly Cys Thr Cys Gly  Cys Thr Gly Cys Ala  Thr 
                1525                 1530                 1535     
Gly Thr Cys Cys  Cys Ala Gly Cys Cys  Ala Cys Thr Gly Thr  Gly Gly 
            1540                 1545                 1550         
Cys Gly Cys  Gly Ala Gly Gly Ala  Cys Cys Thr Gly Thr  Ala Ala 
        1555                 1560                 1565         

