                         LISTAGEM DE SEQUNCIAS

<110>  HELIX SEMENTES E MUDAS LTDA
       EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECURIA
 
<120>  MOLCULA DE CIDO NUCLICO DO EVENTO TRANSGNICO DE MILHO 
       ME240913 EXPRESSANDO A PROTENA CRY1DA, CLULA, PLANTA E SEMENTE 
       TRANSGNICA, USOS DAS MESMAS, PRODUTO DE PLANTA, MTODO, KIT E 
       AMPLICON PARA DETECO DO EVENTO, E MTODOS PARA PRODUZIR UMA 
       PLANTA TRANSGNICA E DE CONTROLE DE INSETOS-PRAGAS LEPIDPTEROS

<130>  Evento ME240913

<140>  BR102019023319-2
<141>  06-11-2019

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1877
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de nucleotdeos codificadora da protena Cry1Da 
       truncada, otimizada para expresso em milho presente no evento 
       ME240913

<400>  1
atggagatca acaaccagaa ccagtgcgtg ccgtacaact gcctgtccaa cccgaaggag       60

atcatcctgg gcgaggagag gctggagacc ggcaacaccg tggccgacat ctccctgggc      120

ctgatcaact tcctgtactc caacttcgtg ccgggcggcg gcttcatcgt gggcctgctg      180

gagctgatct ggggcttcat cggcccgtcc cagtgggaca tcttcctggc ccagatcgag      240

cagctgatct cccagaggat cgaggagttc gccaggaacc aggccatctc caggctggag      300

ggcctgtcca acctgtacaa ggtgtacgtg agggccttct ccgactggga gaaggacccg      360

accaacccgg ccctgaggga ggagatgagg atacagttca acgacatgaa ctccgccctg      420

atcaccgcca tcccgctgtt cagggtgcag aactacgagg tggccctgct gtccgtgtac      480

gtgcaggccg ccaacctgca cctgtccatc ctgagggacg tgtccgtgtt cggcgagagg      540

tggggctacg acaccgccac catcaacaac aggtactccg acctgacctc cctgatccac      600

gtgtacacca accactgcgt ggacacctac aaccagggcc tgaggaggct ggagggcagg      660

ttcctgtccg actggatcgt gtacaacagg ttcaggaggc agctgaccat ctccgtgctg      720

gacatcgtgg ccttcttccc gaactacgac atcaggacct acccgatcca gaccgccacc      780

cagctgacca gggaggtgta cctggacctg ccgttcatca acgagaacct gtccccggcc      840

gcctcctacc cgaccttctc cgccgccgag tccgccatca tcaggtcccc gcacctggtg      900

gacttcctga actccttcac catctacacc gactccctgg ccaggtacgc ctactggggc      960

ggccacctgg tgaactcctt caggaccggc accaccacca acctgatcag gtccccgctg     1020

tacggcaggg agggcaacac cgagaggccg gtgaccatca ccgcctcccc gtccgtgccg     1080

atcttcagga ccctgtccta catcaccggc ctggacaact ccaacccggt ggccggcatc     1140

gagggcgtgg agttccagaa caccatctcc aggtccatct acaggaagtc cggcccgatc     1200

gactccttct ccgagctgcc gccgcaggac gcctccgtgt ccccggccat cggctactcc     1260

cacaggctgt gccacgccac cttcctggag aggatctccg gcccgaggat cgccggcacc     1320

gtgttctcct ggacccacag gtccgcctcc ccgaccaacg aggtgtcccc gtccaggatc     1380

acccagatcc cgtgggtgaa ggcccacacc ctggcctccg gcgcctccgt gatcaagggc     1440

ccgggcttca ccggcggcga catcctgacc aggaactcca tgggcgagct gggcaccctg     1500

agggtgacct tcaccggcag gctgccgcag tcctactaca tcaggttcag gtacgcctcc     1560

gtggccaaca ggtccggcac cttcaggtac tcccagccgc cgtcctacgg catctccttc     1620

ccgaagacca tggacgccgg cgagccgctg acctccaggt ccttcgccca caccaccctg     1680

ttcaccccga tcaccttctc cagggcccag gaggagttcg acctgtacat ccagtccggc     1740

gtgtacatcg acaggatcga gttcatcccg gtgaccgcca ccttcgaggc cgagtacgac     1800

ctggagaggg cccagaaggt ggtgaacgcc ctgttcacct ccaccaacca gctgggcctg     1860

aagaccgacg tgaccta                                                    1877


<210>  2
<211>  5381
<212>  DNA
<213>  Artificial

<220>
<223>  construo completa do transgene do evento ME240913

<400>  2
tattgcactc ccttttaact gttttttatt acaaaaatgc cctggaaaat gcactccctt       60

tttgtgtttg tttttttgtg aaacgatgtt gtcaggtaat ttatttgtca gtctactatg      120

gtggcccatt atattaatag caactgtcgg tccaatagac gacgtcgatt ttctgcattt      180

gtttaaccac gtggatttta tgacatttta tattagttaa tttgtaaaac ctacccaatt      240

aaagacctca tatgttctaa agactaatac ttaatgataa caattttctt ttagtgaaga      300

aagggataat tagtaaatat ggaacaaggg cagaagattt attaaagccg cgtaagagac      360

aacaagtagg tacgtggagt gtcttaggtg acttacccac ataacataaa gtgacattaa      420

caaacatagc taatgctcct atttgaatag tgcatatcag cataccttat tacatataga      480

taggagcaaa ctctagctag attgttgagc agatctcggt gacgggcagg accggacggg      540

gcggtaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct      600

tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca      660

cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca      720

gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg      780

agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg      840

cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc      900

gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga      960

ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg     1020

tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat ggtagatccc ccgttcgtaa     1080

atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga aatgatttaa attgctgcaa     1140

tagaagtaga atgcttgatt gcttgagatt cgtttgtttt gtatatgttg tgttgagaat     1200

taattctcga ggtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg     1260

aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt     1320

gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc     1380

catctttggg accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat     1440

gatggcattt gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag     1500

ctgggcaatg gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg     1560

ccctttggtc ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct     1620

ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt     1680

ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt     1740

caacgatggc ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca     1800

ctatcttcac aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata     1860

ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat     1920

ttttggagta gacaagtgtg tcgtgctcca ccatgttgac ctgcaggcat gcaagcttgt     1980

taacctgcag tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg     2040

tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta     2100

tctatcttta tacatatatt taaactttac tctacgaata atataatcta tagtactaca     2160

ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa     2220

ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct     2280

ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag tacatccatt     2340

tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta     2400

ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt taataattta     2460

gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta     2520

aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg     2580

tcgatcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc     2640

gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc     2700

accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg gcagacgtga     2760

gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg ggggattcct     2820

ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac accccctcca     2880

caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc agatctcccc     2940

caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc cccccccccc     3000

tctctacctt ctctagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct     3060

gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg     3120

gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg     3180

aatcctggga tggctctagc cgttccgcag acgggatcga tctaggatag gtatacatgt     3240

tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc     3300

taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg     3360

atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca     3420

tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt     3480

tacttctgca ggggatccga gctcatggag atcaacaacc agaaccagtg cgtgccgtac     3540

aactgcctgt ccaacccgaa ggagatcatc ctgggcgagg agaggctgga gaccggcaac     3600

accgtggccg acatctccct gggcctgatc aacttcctgt actccaactt cgtgccgggc     3660

ggcggcttca tcgtgggcct gctggagctg atctggggct tcatcggccc gtcccagtgg     3720

gacatcttcc tggcccagat cgagcagctg atctcccaga ggatcgagga gttcgccagg     3780

aaccaggcca tctccaggct ggagggcctg tccaacctgt acaaggtgta cgtgagggcc     3840

ttctccgact gggagaagga cccgaccaac ccggccctga gggaggagat gaggatacag     3900

ttcaacgaca tgaactccgc cctgatcacc gccatcccgc tgttcagggt gcagaactac     3960

gaggtggccc tgctgtccgt gtacgtgcag gccgccaacc tgcacctgtc catcctgagg     4020

gacgtgtccg tgttcggcga gaggtggggc tacgacaccg ccaccatcaa caacaggtac     4080

tccgacctga cctccctgat ccacgtgtac accaaccact gcgtggacac ctacaaccag     4140

ggcctgagga ggctggaggg caggttcctg tccgactgga tcgtgtacaa caggttcagg     4200

aggcagctga ccatctccgt gctggacatc gtggccttct tcccgaacta cgacatcagg     4260

acctacccga tccagaccgc cacccagctg accagggagg tgtacctgga cctgccgttc     4320

atcaacgaga acctgtcccc ggccgcctcc tacccgacct tctccgccgc cgagtccgcc     4380

atcatcaggt ccccgcacct ggtggacttc ctgaactcct tcaccatcta caccgactcc     4440

ctggccaggt acgcctactg gggcggccac ctggtgaact ccttcaggac cggcaccacc     4500

accaacctga tcaggtcccc gctgtacggc agggagggca acaccgagag gccggtgacc     4560

atcaccgcct ccccgtccgt gccgatcttc aggaccctgt cctacatcac cggcctggac     4620

aactccaacc cggtggccgg catcgagggc gtggagttcc agaacaccat ctccaggtcc     4680

atctacagga agtccggccc gatcgactcc ttctccgagc tgccgccgca ggacgcctcc     4740

gtgtccccgg ccatcggcta ctcccacagg ctgtgccacg ccaccttcct ggagaggatc     4800

tccggcccga ggatcgccgg caccgtgttc tcctggaccc acaggtccgc ctccccgacc     4860

aacgaggtgt ccccgtccag gatcacccag atcccgtggg tgaaggccca caccctggcc     4920

tccggcgcct ccgtgatcaa gggcccgggc ttcaccggcg gcgacatcct gaccaggaac     4980

tccatgggcg agctgggcac cctgagggtg accttcaccg gcaggctgcc gcagtcctac     5040

tacatcaggt tcaggtacgc ctccgtggcc aacaggtccg gcaccttcag gtactcccag     5100

ccgccgtcct acggcatctc cttcccgaag accatggacg ccggcgagcc gctgacctcc     5160

aggtccttcg cccacaccac cctgttcacc ccgatcacct tctccagggc ccaggaggag     5220

ttcgacctgt acatccagtc cggcgtgtac atcgacagga tcgagttcat cccggtgacc     5280

gccaccttcg aggccgagta cgacctggag agggcccaga aggtggtgaa cgccctgttc     5340

acctccacca accagctggg cctgaagacc gacgtgacct a                         5381


<210>  3
<211>  625
<212>  PRT
<213>  Artificial

<220>
<223>  protena Cry1Da truncada expressa pelo evento ME240913

<400>  3

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr 
625 


<210>  4
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de juno 5'

<400>  4
aaaaatgccg cgacttgcgg acgt                                              24


<210>  5
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de juno 3'

<400>  5
cccgccttca gtttaaactt tggcatttgt ggaa                                   34


<210>  6
<211>  65
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia flanqueadora 5'

<400>  6
gaggtcgacg gttcgaatac taacaaccgc gtagcgcgcg aattttgcgc gaaaaatgcc       60

gcgac                                                                   65


<210>  7
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia flanqueadora 3'

<400>  7
ttggcatttg tggaatagat cccgagtctc ctacaacaca aggcaagccc cggtgcattt       60

acccttt                                                                 67


<210>  8
<211>  6245
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de nucleotdeos compreendendo a sequncia flanqueadora 
       5, a sequncia completa do inserto e a sequncia flanqueadora 
       3do evento ME240913


<220>
<221>  misc_feature
<222>  (1)..(65)
<223>  sequncia flanqueadora 5

<220>
<221>  misc_feature
<222>  (66)..(6178)
<223>  sequncia completa do inserto

<220>
<221>  misc_feature
<222>  (6179)..(6245)
<223>  sequncia flanqueadora 3

<400>  8
gaggtcgacg gttcgaatac taacaaccgc gtagcgcgcg aattttgcgc gaaaaatgcc       60

gcgacttgcg gacgttttta atgtactgaa ttaacgccga attgctctag cattcgccat      120

tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc      180

tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt      240

cacgacgttg taaaacgacg gccagtgcca agctaattcg cttcaagacg tgctcaaatc      300

actatttcca cacccctata tttctattgc actccctttt aactgttttt tattacaaaa      360

atgccctgga aaatgcactc cctttttgtg tttgtttttt tgtgaaacga tgttgtcagg      420

taatttattt gtcagtctac tatggtggcc cattatatta atagcaactg tcggtccaat      480

agacgacgtc gattttctgc atttgtttaa ccacgtggat tttatgacat tttatattag      540

ttaatttgta aaacctaccc aattaaagac ctcatatgtt ctaaagacta atacttaatg      600

ataacaattt tcttttagtg aagaaaggga taattagtaa atatggaaca agggcagaag      660

atttattaaa gccgcgtaag agacaacaag taggtacgtg gagtgtctta ggtgacttac      720

ccacataaca taaagtgaca ttaacaaaca tagctaatgc tcctatttga atagtgcata      780

tcagcatacc ttattacata tagataggag caaactctag ctagattgtt gagcagatct      840

cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac      900

ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat      960

atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca     1020

cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca     1080

gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg     1140

cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg     1200

cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt     1260

cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc     1320

agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc     1380

tcatggtaga tcccccgttc gtaaatggtg aaaattttca gaaaattgct tttgctttaa     1440

aagaaatgat ttaaattgct gcaatagaag tagaatgctt gattgcttga gattcgtttg     1500

ttttgtatat gttgtgttga gaattaattc tcgaggtcct ctccaaatga aatgaacttc     1560

cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag     1620

tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca     1680

cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggtagag gcatcttgaa     1740

cgatagcctt tcctttatcg caatgatggc atttgtagga gccaccttcc ttttccacta     1800

tcttcacaat aaagtgacag atagctgggc aatggaatcc gaggaggttt ccggatatta     1860

ccctttgttg aaaagtctca attgcccttt ggtcttctga gactgtatct ttgatatttt     1920

tggagtagac aagtgtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag     1980

acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg     2040

gaccactgtc ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt     2100

tgtaggagcc accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat     2160

ggaatccgag gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt     2220

cttctgagac tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt     2280

tgacctgcag gcatgcaagc ttgttaacct gcagtgcagc gtgacccggt cgtgcccctc     2340

tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt     2400

cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg     2460

aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac     2520

agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc     2580

tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc     2640

atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt     2700

ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt     2760

ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt     2820

aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag     2880

ataatgccag cctgttaaac gccgtcgatc gacgagtcta acggacacca accagcgaac     2940

cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg     3000

gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat     3060

tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg     3120

caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc     3180

cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc     3240

acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg     3300

ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg     3360

ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat     3420

ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta     3480

acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga     3540

tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata catgatggca     3600

tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta     3660

tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg     3720

tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc     3780

gatgctcacc ctgttgtttg gtgttacttc tgcaggggat ccgagctcat ggagatcaac     3840

aaccagaacc agtgcgtgcc gtacaactgc ctgtccaacc cgaaggagat catcctgggc     3900

gaggagaggc tggagaccgg caacaccgtg gccgacatct ccctgggcct gatcaacttc     3960

ctgtactcca acttcgtgcc gggcggacct gcagctgatc tcccagagga tcgaggagtt     4020

cgccaggaac caggccatct ccaggctgga gggcctgtcc aacctgtaca aggtgtacgt     4080

gagggccttc tccgactggg agaaggaccc gaccaacccg gccctgaggg aggagatgag     4140

gatacagttc aacgacatga actccgccct gatcaccgcc atcccgctgt tcagggtgca     4200

gaactacgag gtggccctgc tgtccgtgta cgtgcaggcc gccaacctgc acctgtccat     4260

cctgagggac gtgtccgtgt tcggcgagag gtggggctac gacaccgcca ccatcaacaa     4320

caggtactcc gacctgacct ccctgatcca cgtgtacacc aaccactgcg tggacaccta     4380

caaccagggc ctgaggaggc tggagggcag gttcctgtcc gactggatcg tgtacaacag     4440

gttcaggagg cagctgacca tctccgtgct ggacatcgtg gccttcttcc cgaactacga     4500

catcaggacc tacccgatcc agaccgccac ccagctgacc agggaggtgt acctggacct     4560

gccgttcatc aacgagaacc tgtccccggc cgcctcctac ccgaccttct ccgccgccga     4620

gtccgccatc atcaggtccc cgcacctggt ggacttcctg aactccttca ccatctacac     4680

cgactccctg gccaggtacg cctactgggg cggccacctg gtgaactcct tcaggaccgg     4740

caccaccacc aacctgatca ggtccccgct gtacggcagg gagggcaaca ccgagaggcc     4800

ggtgaccatc accgcctccc cgtccgtgcc gatcttcagg accctgtcct acatcaccgg     4860

cctggacaac tccaacccgg tggccggcat cgagggcgtg gagttccaga acaccatctc     4920

caggtccatc tacaggaagt ccggcccgat cgactccttc tccgagctgc cgccgcagga     4980

cgcctccgtg tccccggcca tcggctactc ccacaggctg tgccacgcca ccttcctgga     5040

gaggatctcc ggcccgagga tcgccggcac cgtgttctcc tggacccaca ggtccgcctc     5100

cccgaccaac gaggtgtccc cgtccaggat cacccagatc ccgtgggtga aggcccacac     5160

cctggcctcc ggcgcctccg tgatcaaggg cccgggcttc accggcggcg acatcctgac     5220

caggaactcc atgggcgagc tgggcaccct gagggtgacc ttcaccggca ggctgccgca     5280

gtcctactac atcaggttca ggtacgcctc cgtggccaac aggtccggca ccttcaggta     5340

ctcccagccg ccgtcctacg gcatctcctt cccgaagacc atggacgccg gcgagccgct     5400

gacctccagg tccttcgccc acaccaccct gttcaccccg atcaccttct ccagggccca     5460

ggaggagttc gacctgtaca tccagtccgg cgtgtacatc gacaggatcg agttcatccc     5520

ggtgaccgcc accttcgagg ccgagtacga cctggagagg gcccagaagg tggtgaacgc     5580

cctgttcacc tccaccaacc agctgggcct gaagaccgac gtgacctaag tcgacggatc     5640

cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc     5700

gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg     5760

catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata     5820

cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc     5880

tatgttacta gatcggttaa cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat     5940

tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg     6000

ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag     6060

tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt     6120

ttgcgtattg gagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaacttt     6180

ggcatttgtg gaatagatcc cgagtctcct acaacacaag gcaagccccg gtgcatttac     6240

ccttt                                                                 6245


<210>  9
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador forward til na amplificao da sequncia 
       de juno 5

<400>  9
aggtcgacgg ttcgaatact                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador reverse til na amplificao da sequncia 
       de juno 5

<400>  10
gctagagcaa ttcggcgtta a                                                 21


<210>  11
<211>  110
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia ilustrativa de amplicon resultante de PCR utilizando 
       iniciadores especficos para confirmar a sequncia juno 5

<400>  11
aggtcgacgg ttcgaatact aacaaccgcg tagcgcgcga attttgcgcg aaaaatgccg       60

cgacttgcgg acgtttttaa tgtactgaat taacgccgaa ttgctctagc                 110


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de sonda til na deteco da sequncia de juno 5

<400>  12
acaaccgcgt agcgcgcgaa                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador forward til na amplificao da sequncia 
       de juno 3

<400>  13
cggtttgcgt attggagctt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador reverse til na amplificao da sequncia 
       de juno 3

<400>  14
ggcttgcctt gtgttgtagg                                                   20


<210>  15
<211>  111
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia ilustrativa de amplicon resultante de PCR utilizando 
       iniciadores especficos para confirmar a sequncia juno 3

<400>  15
cggtttgcgt attggagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa       60

ctttggcatt tgtggaatag atcccgagtc tcctacaaca caaggcaagc c               111


<210>  16
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de sonda til na deteco da sequncia de juno 3

<400>  16
tggatcagat tgtcgtttcc cgcct                                             25


