                         SEQUENCE LISTING

<110>  Helix Sementes
       EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECURIA
 
<120>  MOLCULA DE CIDO NUCLICO DO EVENTO TRANSGNICO DE MILHO 
       ME240913 EXPRESSANDO A PROTENA CRY1DA, CLULA, PLANTA E SEMENTE 
       TRANSGNICA, USOS DAS MESMAS, PRODUTO DE PLANTA, MTODO, KIT E 
       AMPLICON PARA DETECO DO EVENTO, E MTODOS PARA PRODUZIR UMA 
       PLANTA TRANSGNICA E DE CONTROLE DE INSETOS-PRAGAS LEPIDPTEROS

<130>  Evento ME240913

<140>  PCT/BR2020/050407
<141>  2020-10-13

<150>  BR102019023319-2
<151>  2019-11-06

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1878
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de nucleotdeos codificadora da protena Cry1Da 
       truncada, otimizada para expresso em milho presente no evento 
       ME240913

<400>  1
atggagatca acaaccagaa ccagtgcgtg ccgtacaact gcctgtccaa cccgaaggag       60

atcatcctgg gcgaggagag gctggagacc ggcaacaccg tggccgacat ctccctgggc      120

ctgatcaact tcctgtactc caacttcgtg ccgggcggcg gcttcatcgt gggcctgctg      180

gagctgatct ggggcttcat cggcccgtcc cagtgggaca tcttcctggc ccagatcgag      240

cagctgatct cccagaggat cgaggagttc gccaggaacc aggccatctc caggctggag      300

ggcctgtcca acctgtacaa ggtgtacgtg agggccttct ccgactggga gaaggacccg      360

accaacccgg ccctgaggga ggagatgagg atacagttca acgacatgaa ctccgccctg      420

atcaccgcca tcccgctgtt cagggtgcag aactacgagg tggccctgct gtccgtgtac      480

gtgcaggccg ccaacctgca cctgtccatc ctgagggacg tgtccgtgtt cggcgagagg      540

tggggctacg acaccgccac catcaacaac aggtactccg acctgacctc cctgatccac      600

gtgtacacca accactgcgt ggacacctac aaccagggcc tgaggaggct ggagggcagg      660

ttcctgtccg actggatcgt gtacaacagg ttcaggaggc agctgaccat ctccgtgctg      720

gacatcgtgg ccttcttccc gaactacgac atcaggacct acccgatcca gaccgccacc      780

cagctgacca gggaggtgta cctggacctg ccgttcatca acgagaacct gtccccggcc      840

gcctcctacc cgaccttctc cgccgccgag tccgccatca tcaggtcccc gcacctggtg      900

gacttcctga actccttcac catctacacc gactccctgg ccaggtacgc ctactggggc      960

ggccacctgg tgaactcctt caggaccggc accaccacca acctgatcag gtccccgctg     1020

tacggcaggg agggcaacac cgagaggccg gtgaccatca ccgcctcccc gtccgtgccg     1080

atcttcagga ccctgtccta catcaccggc ctggacaact ccaacccggt ggccggcatc     1140

gagggcgtgg agttccagaa caccatctcc aggtccatct acaggaagtc cggcccgatc     1200

gactccttct ccgagctgcc gccgcaggac gcctccgtgt ccccggccat cggctactcc     1260

cacaggctgt gccacgccac cttcctggag aggatctccg gcccgaggat cgccggcacc     1320

gtgttctcct ggacccacag gtccgcctcc ccgaccaacg aggtgtcccc gtccaggatc     1380

acccagatcc cgtgggtgaa ggcccacacc ctggcctccg gcgcctccgt gatcaagggc     1440

ccgggcttca ccggcggcga catcctgacc aggaactcca tgggcgagct gggcaccctg     1500

agggtgacct tcaccggcag gctgccgcag tcctactaca tcaggttcag gtacgcctcc     1560

gtggccaaca ggtccggcac cttcaggtac tcccagccgc cgtcctacgg catctccttc     1620

ccgaagacca tggacgccgg cgagccgctg acctccaggt ccttcgccca caccaccctg     1680

ttcaccccga tcaccttctc cagggcccag gaggagttcg acctgtacat ccagtccggc     1740

gtgtacatcg acaggatcga gttcatcccg gtgaccgcca ccttcgaggc cgagtacgac     1800

ctggagaggg cccagaaggt ggtgaacgcc ctgttcacct ccaccaacca gctgggcctg     1860

aagaccgacg tgacctaa                                                   1878


<210>  2
<211>  5647
<212>  DNA
<213>  Artificial

<220>
<223>  construo completa do transgene do evento ME240913

<400>  2
tattgcactc ccttttaact gttttttatt acaaaaatgc cctggaaaat gcactccctt       60

tttgtgtttg tttttttgtg aaacgatgtt gtcaggtaat ttatttgtca gtctactatg      120

gtggcccatt atattaatag caactgtcgg tccaatagac gacgtcgatt ttctgcattt      180

gtttaaccac gtggatttta tgacatttta tattagttaa tttgtaaaac ctacccaatt      240

aaagacctca tatgttctaa agactaatac ttaatgataa caattttctt ttagtgaaga      300

aagggataat tagtaaatat ggaacaaggg cagaagattt attaaagccg cgtaagagac      360

aacaagtagg tacgtggagt gtcttaggtg acttacccac ataacataaa gtgacattaa      420

caaacatagc taatgctcct atttgaatag tgcatatcag cataccttat tacatataga      480

taggagcaaa ctctagctag attgttgagc agatctcggt gacgggcagg accggacggg      540

gcggtaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct      600

tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca      660

cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca      720

gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg      780

agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg      840

cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc      900

gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga      960

ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg     1020

tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat ggtagatccc ccgttcgtaa     1080

atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga aatgatttaa attgctgcaa     1140

tagaagtaga atgcttgatt gcttgagatt cgtttgtttt gtatatgttg tgttgagaat     1200

taattctcga ggtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg     1260

aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt     1320

gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc     1380

catctttggg accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat     1440

gatggcattt gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag     1500

ctgggcaatg gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg     1560

ccctttggtc ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct     1620

ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt     1680

ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt     1740

caacgatggc ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca     1800

ctatcttcac aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata     1860

ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat     1920

ttttggagta gacaagtgtg tcgtgctcca ccatgttgac ctgcaggcat gcaagcttgt     1980

taacctgcag tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg     2040

tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta     2100

tctatcttta tacatatatt taaactttac tctacgaata atataatcta tagtactaca     2160

ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa     2220

ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct     2280

ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag tacatccatt     2340

tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta     2400

ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt taataattta     2460

gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta     2520

aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg     2580

tcgatcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc     2640

gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc     2700

accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg gcagacgtga     2760

gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg ggggattcct     2820

ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac accccctcca     2880

caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc agatctcccc     2940

caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc cccccccccc     3000

tctctacctt ctctagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct     3060

gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg     3120

gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg     3180

aatcctggga tggctctagc cgttccgcag acgggatcga tctaggatag gtatacatgt     3240

tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc     3300

taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg     3360

atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca     3420

tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt     3480

tacttctgca ggggatccga gctcatggag atcaacaacc agaaccagtg cgtgccgtac     3540

aactgcctgt ccaacccgaa ggagatcatc ctgggcgagg agaggctgga gaccggcaac     3600

accgtggccg acatctccct gggcctgatc aacttcctgt actccaactt cgtgccgggc     3660

ggcggcttca tcgtgggcct gctggagctg atctggggct tcatcggccc gtcccagtgg     3720

gacatcttcc tggcccagat cgagcagctg atctcccaga ggatcgagga gttcgccagg     3780

aaccaggcca tctccaggct ggagggcctg tccaacctgt acaaggtgta cgtgagggcc     3840

ttctccgact gggagaagga cccgaccaac ccggccctga gggaggagat gaggatacag     3900

ttcaacgaca tgaactccgc cctgatcacc gccatcccgc tgttcagggt gcagaactac     3960

gaggtggccc tgctgtccgt gtacgtgcag gccgccaacc tgcacctgtc catcctgagg     4020

gacgtgtccg tgttcggcga gaggtggggc tacgacaccg ccaccatcaa caacaggtac     4080

tccgacctga cctccctgat ccacgtgtac accaaccact gcgtggacac ctacaaccag     4140

ggcctgagga ggctggaggg caggttcctg tccgactgga tcgtgtacaa caggttcagg     4200

aggcagctga ccatctccgt gctggacatc gtggccttct tcccgaacta cgacatcagg     4260

acctacccga tccagaccgc cacccagctg accagggagg tgtacctgga cctgccgttc     4320

atcaacgaga acctgtcccc ggccgcctcc tacccgacct tctccgccgc cgagtccgcc     4380

atcatcaggt ccccgcacct ggtggacttc ctgaactcct tcaccatcta caccgactcc     4440

ctggccaggt acgcctactg gggcggccac ctggtgaact ccttcaggac cggcaccacc     4500

accaacctga tcaggtcccc gctgtacggc agggagggca acaccgagag gccggtgacc     4560

atcaccgcct ccccgtccgt gccgatcttc aggaccctgt cctacatcac cggcctggac     4620

aactccaacc cggtggccgg catcgagggc gtggagttcc agaacaccat ctccaggtcc     4680

atctacagga agtccggccc gatcgactcc ttctccgagc tgccgccgca ggacgcctcc     4740

gtgtccccgg ccatcggcta ctcccacagg ctgtgccacg ccaccttcct ggagaggatc     4800

tccggcccga ggatcgccgg caccgtgttc tcctggaccc acaggtccgc ctccccgacc     4860

aacgaggtgt ccccgtccag gatcacccag atcccgtggg tgaaggccca caccctggcc     4920

tccggcgcct ccgtgatcaa gggcccgggc ttcaccggcg gcgacatcct gaccaggaac     4980

tccatgggcg agctgggcac cctgagggtg accttcaccg gcaggctgcc gcagtcctac     5040

tacatcaggt tcaggtacgc ctccgtggcc aacaggtccg gcaccttcag gtactcccag     5100

ccgccgtcct acggcatctc cttcccgaag accatggacg ccggcgagcc gctgacctcc     5160

aggtccttcg cccacaccac cctgttcacc ccgatcacct tctccagggc ccaggaggag     5220

ttcgacctgt acatccagtc cggcgtgtac atcgacagga tcgagttcat cccggtgacc     5280

gccaccttcg aggccgagta cgacctggag agggcccaga aggtggtgaa cgccctgttc     5340

acctccacca accagctggg cctgaagacc gacgtgacct aagtcgacgg atccgatcgt     5400

tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt     5460

atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg     5520

ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata     5580

gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta     5640

ctagatc                                                               5647


<210>  3
<211>  625
<212>  PRT
<213>  Artificial

<220>
<223>  protena Cry1Da truncada expressa pelo evento ME240913

<400>  3

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr 
625 


<210>  4
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de juno 5'

<400>  4
aaaaatgccg cgacttgcgg acgt                                              24


<210>  5
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de juno 3'

<400>  5
cccgccttca gtttaaactt tggcatttgt ggaa                                   34


<210>  6
<211>  116
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia flanqueadora 5'

<400>  6
gacacaagac atgtcataga cggagtggta ggggagggta cgcgcgaggg tgaggtcgac       60

ggttcgaata ctaacaaccg cgtagcgcgc gaattttgcg cgaaaaatgc cgcgac          116


<210>  7
<211>  118
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia flanqueadora 3'

<400>  7
ttggcatttg tggaatagat cccgagtctc ctacaacaca aggcaagccc cggtgcattt       60

accctttcct tgtgttttta aactccttat cacttgaatt gatgcattag gtgatagg        118


<210>  8
<211>  6424
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de nucleotdeos compreendendo a sequncia flanqueadora 
       5, a sequncia completa do inserto e a sequncia flanqueadora 3
       do evento ME240913


<220>
<221>  misc_feature
<222>  (1)..(116)
<223>  sequncia flanqueadora 5

<220>
<221>  misc_feature
<222>  (117)..(6306)
<223>  sequncia completa do inserto

<220>
<221>  misc_feature
<222>  (6307)..(6424)
<223>  sequncia flanqueadora 3

<400>  8
gacacaagac atgtcataga cggagtggta ggggagggta cgcgcgaggg tgaggtcgac       60

ggttcgaata ctaacaaccg cgtagcgcgc gaattttgcg cgaaaaatgc cgcgacttgc      120

ggacgttttt aatgtactga attaacgccg aattgctcta gcattcgcca ttcaggctgc      180

gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag      240

ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt      300

gtaaaacgac ggccagtgcc aagctaattc gcttcaagac gtgctcaaat cactatttcc      360

acacccctat atttctattg cactcccttt taactgtttt ttattacaaa aatgccctgg      420

aaaatgcact ccctttttgt gtttgttttt ttgtgaaacg atgttgtcag gtaatttatt      480

tgtcagtcta ctatggtggc ccattatatt aatagcaact gtcggtccaa tagacgacgt      540

cgattttctg catttgttta accacgtgga ttttatgaca ttttatatta gttaatttgt      600

aaaacctacc caattaaaga cctcatatgt tctaaagact aatacttaat gataacaatt      660

ttcttttagt gaagaaaggg ataattagta aatatggaac aagggcagaa gatttattaa      720

agccgcgtaa gagacaacaa gtaggtacgt ggagtgtctt aggtgactta cccacataac      780

ataaagtgac attaacaaac atagctaatg ctcctatttg aatagtgcat atcagcatac      840

cttattacat atagatagga gcaaactcta gctagattgt tgagcagatc tcggtgacgg      900

gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat      960

gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg     1020

cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga     1080

agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc     1140

gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg     1200

ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc     1260

agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct     1320

cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg     1380

gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag     1440

atcccccgtt cgtaaatggt gaaaattttc agaaaattgc ttttgcttta aaagaaatga     1500

tttaaattgc tgcaatagaa gtagaatgct tgattgcttg agattcgttt gttttgtata     1560

tgttgtgttg agaattaatt ctcgaggtcc tctccaaatg aaatgaactt ccttatatag     1620

aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat     1680

cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc     1740

tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct     1800

ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa     1860

taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt     1920

gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga     1980

caagtgtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg     2040

gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt     2100

cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc     2160

caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga     2220

ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga     2280

ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacctgca     2340

ggcatgcaag cttgttaacc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat     2400

aatgagcatt gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt     2460

ttgaagtgca gtttatctat ctttatacat atatttaaac tttactctac gaataatata     2520

atctatagta ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca     2580

tggtctaaag gacaattgag tattttgaca acaggactct acagttttat ctttttagtg     2640

tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt     2700

attagtacat ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac     2760

atctatttta ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt     2820

ttatttaata atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata     2880

ccctttaaga aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca     2940

gcctgttaaa cgccgtcgat cgacgagtct aacggacacc aaccagcgaa ccagcagcgt     3000

cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct     3060

cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg     3120

gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag     3180

ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa     3240

tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca     3300

caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc     3360

ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc     3420

ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc     3480

tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag     3540

tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatctag     3600

gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca     3660

tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata     3720

attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt     3780

ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac     3840

cctgttgttt ggtgttactt ctgcagggga tccgagctca tggagatcaa caaccagaac     3900

cagtgcgtgc cgtacaactg cctgtccaac ccgaaggaga tcatcctggg cgaggagagg     3960

ctggagaccg gcaacaccgt ggccgacatc tccctgggcc tgatcaactt cctgtactcc     4020

aacttcgtgc cgggcggcgg cttcatcgtg ggcctgctgg agctgatctg gggcttcatc     4080

ggcccgtccc agtgggacat cttcctggcc cagatcgagc agctgatctc ccagaggatc     4140

gaggagttcg ccaggaacca ggccatctcc aggctggagg gcctgtccaa cctgtacaag     4200

gtgtacgtga gggccttctc cgactgggag aaggacccga ccaacccggc cctgagggag     4260

gagatgagga tacagttcaa cgacatgaac tccgccctga tcaccgccat cccgctgttc     4320

agggtgcaga actacgaggt ggccctgctg tccgtgtacg tgcaggccgc caacctgcac     4380

ctgtccatcc tgagggacgt gtccgtgttc ggcgagaggt ggggctacga caccgccacc     4440

atcaacaaca ggtactccga cctgacctcc ctgatccacg tgtacaccaa ccactgcgtg     4500

gacacctaca accagggcct gaggaggctg gagggcaggt tcctgtccga ctggatcgtg     4560

tacaacaggt tcaggaggca gctgaccatc tccgtgctgg acatcgtggc cttcttcccg     4620

aactacgaca tcaggaccta cccgatccag accgccaccc agctgaccag ggaggtgtac     4680

ctggacctgc cgttcatcaa cgagaacctg tccccggccg cctcctaccc gaccttctcc     4740

gccgccgagt ccgccatcat caggtccccg cacctggtgg acttcctgaa ctccttcacc     4800

atctacaccg actccctggc caggtacgcc tactggggcg gccacctggt gaactccttc     4860

aggaccggca ccaccaccaa cctgatcagg tccccgctgt acggcaggga gggcaacacc     4920

gagaggccgg tgaccatcac cgcctccccg tccgtgccga tcttcaggac cctgtcctac     4980

atcaccggcc tggacaactc caacccggtg gccggcatcg agggcgtgga gttccagaac     5040

accatctcca ggtccatcta caggaagtcc ggcccgatcg actccttctc cgagctgccg     5100

ccgcaggacg cctccgtgtc cccggccatc ggctactccc acaggctgtg ccacgccacc     5160

ttcctggaga ggatctccgg cccgaggatc gccggcaccg tgttctcctg gacccacagg     5220

tccgcctccc cgaccaacga ggtgtccccg tccaggatca cccagatccc gtgggtgaag     5280

gcccacaccc tggcctccgg cgcctccgtg atcaagggcc cgggcttcac cggcggcgac     5340

atcctgacca ggaactccat gggcgagctg ggcaccctga gggtgacctt caccggcagg     5400

ctgccgcagt cctactacat caggttcagg tacgcctccg tggccaacag gtccggcacc     5460

ttcaggtact cccagccgcc gtcctacggc atctccttcc cgaagaccat ggacgccggc     5520

gagccgctga cctccaggtc cttcgcccac accaccctgt tcaccccgat caccttctcc     5580

agggcccagg aggagttcga cctgtacatc cagtccggcg tgtacatcga caggatcgag     5640

ttcatcccgg tgaccgccac cttcgaggcc gagtacgacc tggagagggc ccagaaggtg     5700

gtgaacgccc tgttcacctc caccaaccag ctgggcctga agaccgacgt gacctaagtc     5760

gacggatccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc     5820

ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac     5880

atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac     5940

atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg     6000

gtgtcatcta tgttactaga tcggttaacg aattcgtaat catgtcatag ctgtttcctg     6060

tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta     6120

aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg     6180

ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga     6240

gaggcggttt gcgtattgga gcttgagctt ggatcagatt gtcgtttccc gccttcagtt     6300

taaactttgg catttgtgga atagatcccg agtctcctac aacacaaggc aagccccggt     6360

gcatttaccc tttccttgtg tttttaaact ccttatcact tgaattgatg cattaggtga     6420

tagg                                                                  6424


<210>  9
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador forward til na amplificao da sequncia 
       de juno 5

<400>  9
aggtcgacgg ttcgaatact                                                   20


<210>  10
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador reverse til na amplificao da sequncia 
       de juno 5

<400>  10
gctagagcaa ttcggcgtta a                                                 21


<210>  11
<211>  110
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia ilustrativa de amplicon resultante de PCR utilizando 
       iniciadores especficos para confirmar a sequncia juno 5

<400>  11
aggtcgacgg ttcgaatact aacaaccgcg tagcgcgcga attttgcgcg aaaaatgccg       60

cgacttgcgg acgtttttaa tgtactgaat taacgccgaa ttgctctagc                 110


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de sonda til na deteco da sequncia de juno 5

<400>  12
acaaccgcgt agcgcgcgaa                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador forward til na amplificao da sequncia 
       de juno 3

<400>  13
cggtttgcgt attggagctt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de iniciador reverse til na amplificao da sequncia 
       de juno 3

<400>  14
ggcttgcctt gtgttgtagg                                                   20


<210>  15
<211>  111
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia ilustrativa de amplicon resultante de PCR utilizando 
       iniciadores especficos para confirmar a sequncia juno 3

<400>  15
cggtttgcgt attggagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa       60

ctttggcatt tgtggaatag atcccgagtc tcctacaaca caaggcaagc c               111


<210>  16
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  sequncia de sonda til na deteco da sequncia de juno 3

<400>  16
tggatcagat tgtcgtttcc cgcct                                             25


