                         SEQUENCE LISTING

<110>  iNtRON Biotechnology, Co. Ltd.
 
<120>  Novel antibacterial protein CPEL-1 having lytic activity against 
       Clostridium perfringens

<130>  KP090679

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1167
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Gene coding antibacterial protein CPEL-1

<400>  1
atgaaaatca ataaaagatt aagtactact aatgttactc tagatgcaaa taatccagag       60

tatataatta tccacgaaac tgataatacc tcttggggtg ctggagctga aacccactgt      120

aaggcccaag caaatggaaa tataggtaaa gccagtgtcc attattatgt cgatgataca      180

ggggtatatc aagctgtaga acacaaacat gccacttgga attgtggaga tggtcataat      240

agatatggta tcaataatag aaatacaata tctatagaaa tatgtgttaa tcctgactct      300

gactacaata gagcagttga taatgctgta gagctagtta gatatctaaa gaatggctac      360

tattctaatt gtaaagtagt aagacactat gatgccagta gaaaaaattg tcctagaaga      420

atgatagcca atggttactg gaatacattc ctagaaagag taaattcagg agagagctca      480

agctcaccaa taatcaataa ccctaaagga ttctatgagt ctaatgagac aagaactaat      540

gctactttag taggggaagg ttcaatagaa gttctagatg aggattgtaa gccagtccct      600

ggtagattta ttgatagcct agataattta tttgttcttg gaatatatcc ttccagaaat      660

ttcatagaag ttgtgtatcc tggaaaggat aagaaatacc atgcttacat tgacataaaa      720

cattatagca gattaagttt tgactaccac atgaagtatc aaaatgataa tggaataact      780

tatgtgtggt ggaaccctga agacattaat gtcaaagatc ataatgaaga attacagcct      840

ggtcaaaagg ctagcccaat gtatagaact aagggctggt taagaataac attttataga      900

aaggatggta ctccatctga tggatacgtt cgttacgagg gtgagcaaag ccagaaattc      960

tatgaggatg ttaaacaagg aatagttaag gttaacactt ctcttaatgt aagagatgat     1020

gtgaatggta atataatagg ctcagtatct aataatgaga gagttactat attaggaagt     1080

aagaatggtt ggtatcatat agaatataat actagccatg gtaagaagca aggttatgta     1140

agttcaaaat atgtagagat aatttag                                         1167


<210>  2
<211>  388
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Antibacterial protein CPEL-1

<400>  2

Met Lys Ile Asn Lys Arg Leu Ser Thr Thr Asn Val Thr Leu Asp Ala 
1               5                   10                  15      


Asn Asn Pro Glu Tyr Ile Ile Ile His Glu Thr Asp Asn Thr Ser Trp 
            20                  25                  30          


Gly Ala Gly Ala Glu Thr His Cys Lys Ala Gln Ala Asn Gly Asn Ile 
        35                  40                  45              


Gly Lys Ala Ser Val His Tyr Tyr Val Asp Asp Thr Gly Val Tyr Gln 
    50                  55                  60                  


Ala Val Glu His Lys His Ala Thr Trp Asn Cys Gly Asp Gly His Asn 
65                  70                  75                  80  


Arg Tyr Gly Ile Asn Asn Arg Asn Thr Ile Ser Ile Glu Ile Cys Val 
                85                  90                  95      


Asn Pro Asp Ser Asp Tyr Asn Arg Ala Val Asp Asn Ala Val Glu Leu 
            100                 105                 110         


Val Arg Tyr Leu Lys Asn Gly Tyr Tyr Ser Asn Cys Lys Val Val Arg 
        115                 120                 125             


His Tyr Asp Ala Ser Arg Lys Asn Cys Pro Arg Arg Met Ile Ala Asn 
    130                 135                 140                 


Gly Tyr Trp Asn Thr Phe Leu Glu Arg Val Asn Ser Gly Glu Ser Ser 
145                 150                 155                 160 


Ser Ser Pro Ile Ile Asn Asn Pro Lys Gly Phe Tyr Glu Ser Asn Glu 
                165                 170                 175     


Thr Arg Thr Asn Ala Thr Leu Val Gly Glu Gly Ser Ile Glu Val Leu 
            180                 185                 190         


Asp Glu Asp Cys Lys Pro Val Pro Gly Arg Phe Ile Asp Ser Leu Asp 
        195                 200                 205             


Asn Leu Phe Val Leu Gly Ile Tyr Pro Ser Arg Asn Phe Ile Glu Val 
    210                 215                 220                 


Val Tyr Pro Gly Lys Asp Lys Lys Tyr His Ala Tyr Ile Asp Ile Lys 
225                 230                 235                 240 


His Tyr Ser Arg Leu Ser Phe Asp Tyr His Met Lys Tyr Gln Asn Asp 
                245                 250                 255     


Asn Gly Ile Thr Tyr Val Trp Trp Asn Pro Glu Asp Ile Asn Val Lys 
            260                 265                 270         


Asp His Asn Glu Glu Leu Gln Pro Gly Gln Lys Ala Ser Pro Met Tyr 
        275                 280                 285             


Arg Thr Lys Gly Trp Leu Arg Ile Thr Phe Tyr Arg Lys Asp Gly Thr 
    290                 295                 300                 


Pro Ser Asp Gly Tyr Val Arg Tyr Glu Gly Glu Gln Ser Gln Lys Phe 
305                 310                 315                 320 


Tyr Glu Asp Val Lys Gln Gly Ile Val Lys Val Asn Thr Ser Leu Asn 
                325                 330                 335     


Val Arg Asp Asp Val Asn Gly Asn Ile Ile Gly Ser Val Ser Asn Asn 
            340                 345                 350         


Glu Arg Val Thr Ile Leu Gly Ser Lys Asn Gly Trp Tyr His Ile Glu 
        355                 360                 365             


Tyr Asn Thr Ser His Gly Lys Lys Gln Gly Tyr Val Ser Ser Lys Tyr 
    370                 375                 380                 


Val Glu Ile Ile 
385             


<210>  3
<211>  3685
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Gene coding Escherichia coli pBAD-CPEL-1-TOP10 to produce 
       antibacterial protein CPEL-1

<400>  3
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctagaaataa ttttgtttaa ctttaagaag gagatataca tccaagctta tgaaaatcaa      360

taaaagatta agtactacta atgttactct agatgcaaat aatccagagt atataattat      420

ccacgaaact gataatacct cttggggtgc tggagctgaa acccactgta aggcccaagc      480

aaatggaaat ataggtaaag ccagtgtcca ttattatgtc gatgatacag gggtatatca      540

agctgtagaa cacaaacatg ccacttggaa ttgtggagat ggtcataata gatatggtat      600

caataataga aatacaatat ctatagaaat atgtgttaat cctgactctg actacaatag      660

agcagttgat aatgctgtag agctagttag atatctaaag aatggctact attctaattg      720

taaagtagta agacactatg atgccagtag aaaaaattgt cctagaagaa tgatagccaa      780

tggttactgg aatacattcc tagaaagagt aaattcagga gagagctcaa gctcaccaat      840

aatcaataac cctaaaggat tctatgagtc taatgagaca agaactaatg ctactttagt      900

aggggaaggt tcaatagaag ttctagatga ggattgtaag ccagtccctg gtagatttat      960

tgatagccta gataatttat ttgttcttgg aatatatcct tccagaaatt tcatagaagt     1020

tgtgtatcct ggaaaggata agaaatacca tgcttacatt gacataaaac attatagcag     1080

attaagtttt gactaccaca tgaagtatca aaatgataat ggaataactt atgtgtggtg     1140

gaaccctgaa gacattaatg tcaaagatca taatgaagaa ttacagcctg gtcaaaaggc     1200

tagcccaatg tatagaacta agggctggtt aagaataaca ttttatagaa aggatggtac     1260

tccatctgat ggatacgttc gttacgaggg tgagcaaagc cagaaattct atgaggatgt     1320

taaacaagga atagttaagg ttaacacttc tcttaatgta agagatgatg tgaatggtaa     1380

tataataggc tcagtatcta ataatgagag agttactata ttaggaagta agaatggttg     1440

gtatcatata gaatataata ctagccatgg taagaagcaa ggttatgtaa gttcaaaata     1500

tgtagagata atttaggcgg ccgcaagggc gagcttgaag gtaagcctat ccctaaccct     1560

ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacg     1620

gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat     1680

cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc     1740

cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt     1800

ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa     1860

gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat     1920

ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc     1980

ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt     2040

gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat     2100

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     2160

aatctaaagt atatatgagt aaacttggtc tgacagttag gcgtcgcttg gtcggtcatt     2220

tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct     2280

gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa     2340

gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca     2400

gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc     2460

aggcatcgcc atgtgtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg     2520

cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa     2580

gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg     2640

ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt     2700

tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca     2760

gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg     2820

tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt     2880

cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag     2940

agcagccgat tgtcagttgt gcccagtcat agccgaatag cctctccacc caagcggccg     3000

gagaacctgc gtgcaatcca tcttgttcaa tcatactctt cctttttcaa tattattgaa     3060

gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact     3120

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     3180

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     3240

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     3300

ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     3360

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     3420

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     3480

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     3540

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     3600

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     3660

atctttatag tcctgtcggg tttcg                                           3685


