                         SEQUENCE LISTING

<110>  Beijing Biocytogen Co., Ltd.
 
<120>  GENETICALLY MODIFIED NON-HUMAN ANIMALS WITH HUMAN OR CHIMERIC THPO

<130>  1

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  2262
<212>  DNA
<213>  Mouse

<400>  1
gaggaggggc tgtaccccaa ccagtgtctt cctacctact tgttccccag agtgctgcct       60

gctgtgcacc tgggtcctga ggcccttctc cacccggaca gagtccttgg cccacctctc      120

tcccacccga ctctgccgaa agaagcacag aagctcaagc cgcctccatg gccccaggaa      180

agattcaggg gagaggcccc atacagggag ccacttcagt tagacaccct ggccagaatg      240

gagctgactg atttgctcct ggcggccatg cttcttgcag tggcaagact aactctgtcc      300

agccccgtag ctcctgcctg tgaccccaga ctcctaaata aactgctgcg tgactcccac      360

ctccttcaca gccgactgag tcagtgtccc gacgtcgacc ctttgtctat ccctgttctg      420

ctgcctgctg tggactttag cctgggagaa tggaaaaccc agacggaaca gagcaaggca      480

caggacattc taggggcagt gtcccttcta ctggagggag tgatggcagc acgaggacag      540

ttggaaccct cctgcctctc atccctcctg ggacagcttt ctgggcaggt tcgcctcctc      600

ttgggggccc tgcagggcct cctaggaacc cagcttcctc tacagggcag gaccacagct      660

cacaaggacc ccaatgccct cttcttgagc ttgcaacaac tgcttcgggg aaaggtgcgc      720

ttcctgcttc tggtagaagg tcccaccctc tgtgtcagac ggaccctgcc aaccacagct      780

gtcccaagca gtacttctca actcctcaca ctaaacaagt tcccaaacag gacttctgga      840

ttgttggaga cgaacttcag tgtcacagcc agaactgctg gccctggact tctgagcagg      900

cttcagggat tcagagtcaa gattactcct ggtcagctaa atcaaacctc caggtcccca      960

gtccaaatct ctggatacct gaacaggaca cacggacctg tgaatggaac tcatgggctc     1020

tttgctggaa cctcacttca gaccctggaa gcctcagaca tctcgcccgg agctttcaac     1080

aaaggctccc tggcattcaa cctccagggt ggacttcctc cttctccaag ccttgctcct     1140

gatggacaca cacccttccc tccttcacct gccttgccca ccacccatgg atctccaccc     1200

cagctccacc ccctgtttcc tgacccttcc accaccatgc ctaactctac cgcccctcat     1260

ccagtcacaa tgtaccctca tcccaggaat ttgtctcagg aaacatagcg cgggcactgg     1320

cccagtgagc gtctgcagct tctctcgggg acaagcttcc ccaggaaggc tgagaggcag     1380

ctgcatctgc tccagatgtt ctgctttcac ctaaaaggcc ctggggaagg gatacacagc     1440

actggagatt gtaaaatttt aggagctatt tttttttaac ctatcagcaa tattcatcag     1500

agcagctagc gatctttggt ctattttcgg tataaatttg aaaatcacta attctctata     1560

tgtgctttca tacattaagt ctgcaaatgc ctaggcaggt cttgcctttt aacccaggta     1620

gatgctacac catatcagaa aacaaaaggg aactttcctt tgcttcaagt ttaagccttc     1680

ccgcgcctcc aacaccccca caatgccctc cttcccttca ctgtaattct cagtgagact     1740

gtatggtcct cgagatatac tgctcttgat aaagaattaa caggctatca cttagaaaga     1800

ctgtccctac ttaagagaca aaactgaacc tgtaagagaa taactgggag cacccaaaag     1860

ccaataaaaa tcagggacca atgttcttca ctggggcaac agagctcctg gaccctgcct     1920

cccaagaaag ctaacaggaa gcctgggagc tccacacccc aggtaaggct gtgcagctgg     1980

ctcagtaaag agcagacttg gatgtggcag ctgagcaaag agcatcagca gctcagcagg     2040

ggctcagcca ggcctgggct cctgcttccc tcctgtggag gtcaggcgga agtgcaggaa     2100

gtggcaagag gcaggctcct cggctcacac agcaggacaa gcacagagcg cttgaaggct     2160

ctttataatt tccacaaatg cacctaaaaa gcagccctgt gtggccactc caaactttat     2220

tggaaccctc ccaaatgaag gcagacttat aggactttcc aa                        2262


<210>  2
<211>  356
<212>  PRT
<213>  Mouse

<400>  2

Met Glu Leu Thr Asp Leu Leu Leu Ala Ala Met Leu Leu Ala Val Ala 
1               5                   10                  15      


Arg Leu Thr Leu Ser Ser Pro Val Ala Pro Ala Cys Asp Pro Arg Leu 
            20                  25                  30          


Leu Asn Lys Leu Leu Arg Asp Ser His Leu Leu His Ser Arg Leu Ser 
        35                  40                  45              


Gln Cys Pro Asp Val Asp Pro Leu Ser Ile Pro Val Leu Leu Pro Ala 
    50                  55                  60                  


Val Asp Phe Ser Leu Gly Glu Trp Lys Thr Gln Thr Glu Gln Ser Lys 
65                  70                  75                  80  


Ala Gln Asp Ile Leu Gly Ala Val Ser Leu Leu Leu Glu Gly Val Met 
                85                  90                  95      


Ala Ala Arg Gly Gln Leu Glu Pro Ser Cys Leu Ser Ser Leu Leu Gly 
            100                 105                 110         


Gln Leu Ser Gly Gln Val Arg Leu Leu Leu Gly Ala Leu Gln Gly Leu 
        115                 120                 125             


Leu Gly Thr Gln Leu Pro Leu Gln Gly Arg Thr Thr Ala His Lys Asp 
    130                 135                 140                 


Pro Asn Ala Leu Phe Leu Ser Leu Gln Gln Leu Leu Arg Gly Lys Val 
145                 150                 155                 160 


Arg Phe Leu Leu Leu Val Glu Gly Pro Thr Leu Cys Val Arg Arg Thr 
                165                 170                 175     


Leu Pro Thr Thr Ala Val Pro Ser Ser Thr Ser Gln Leu Leu Thr Leu 
            180                 185                 190         


Asn Lys Phe Pro Asn Arg Thr Ser Gly Leu Leu Glu Thr Asn Phe Ser 
        195                 200                 205             


Val Thr Ala Arg Thr Ala Gly Pro Gly Leu Leu Ser Arg Leu Gln Gly 
    210                 215                 220                 


Phe Arg Val Lys Ile Thr Pro Gly Gln Leu Asn Gln Thr Ser Arg Ser 
225                 230                 235                 240 


Pro Val Gln Ile Ser Gly Tyr Leu Asn Arg Thr His Gly Pro Val Asn 
                245                 250                 255     


Gly Thr His Gly Leu Phe Ala Gly Thr Ser Leu Gln Thr Leu Glu Ala 
            260                 265                 270         


Ser Asp Ile Ser Pro Gly Ala Phe Asn Lys Gly Ser Leu Ala Phe Asn 
        275                 280                 285             


Leu Gln Gly Gly Leu Pro Pro Ser Pro Ser Leu Ala Pro Asp Gly His 
    290                 295                 300                 


Thr Pro Phe Pro Pro Ser Pro Ala Leu Pro Thr Thr His Gly Ser Pro 
305                 310                 315                 320 


Pro Gln Leu His Pro Leu Phe Pro Asp Pro Ser Thr Thr Met Pro Asn 
                325                 330                 335     


Ser Thr Ala Pro His Pro Val Thr Met Tyr Pro His Pro Arg Asn Leu 
            340                 345                 350         


Ser Gln Glu Thr 
        355     


<210>  3
<211>  1918
<212>  DNA
<213>  human

<400>  3
agaagtggcc caggcaggcg tatgacctgc tgctgtggag gggctgtgcc ccaccgccac       60

atgtcttcct acccatctgc tccccagagg gctgcctgct gtgcacttgg gtcctggagc      120

ccttctccac ccggatagat tcttcaccct tggcccgcct ttgccccacc ctactctgcc      180

cagaagtgca agagcctaag ccgcctccat ggccccagga aggattcagg ggagaggccc      240

caaacaggga gccacgccag ccagacaccc cggccagaat ggagctgact gaattgctcc      300

tcgtggtcat gcttctccta actgcaaggc taacgctgtc cagcccggct cctcctgctt      360

gtgacctccg agtcctcagt aaactgcttc gtgactccca tgtccttcac agcagactga      420

gccagtgccc agaggttcac cctttgccta cacctgtcct gctgcctgct gtggacttta      480

gcttgggaga atggaaaacc cagatggagg agaccaaggc acaggacatt ctgggagcag      540

tgacccttct gctggaggga gtgatggcag cacggggaca actgggaccc acttgcctct      600

catccctcct ggggcagctt tctggacagg tccgtctcct ccttggggcc ctgcagagcc      660

tccttggaac ccagcttcct ccacagggca ggaccacagc tcacaaggat cccaatgcca      720

tcttcctgag cttccaacac ctgctccgag gaaaggtgcg tttcctgatg cttgtaggag      780

ggtccaccct ctgcgtcagg cgggccccac ccaccacagc tgtccccagc agaacctctc      840

tagtcctcac actgaacgag ctcccaaaca ggacttctgg attgttggag acaaacttca      900

ctgcctcagc cagaactact ggctctgggc ttctgaagtg gcagcaggga ttcagagcca      960

agattcctgg tctgctgaac caaacctcca ggtccctgga ccaaatcccc ggatacctga     1020

acaggataca cgaactcttg aatggaactc gtggactctt tcctggaccc tcacgcagga     1080

ccctaggagc cccggacatt tcctcaggaa catcagacac aggctccctg ccacccaacc     1140

tccagcctgg atattctcct tccccaaccc atcctcctac tggacagtat acgctcttcc     1200

ctcttccacc caccttgccc acccctgtgg tccagctcca ccccctgctt cctgaccctt     1260

ctgctccaac gcccacccct accagccctc ttctaaacac atcctacacc cactcccaga     1320

atctgtctca ggaagggtaa ggttctcaga cactgccgac atcagcattg tctcgtgtac     1380

agctcccttc cctgcagggc gcccctggga gacaactgga caagatttcc tactttctcc     1440

tgaaacccaa agccctggta aaagggatac acaggactga aaagggaatc atttttcact     1500

gtacattata aaccttcaga agctattttt ttaagctatc agcaatactc atcagagcag     1560

ctagctcttt ggtctatttt ctgcagaaat ttgcaactca ctgattctct acatgctctt     1620

tttctgtgat aactctgcaa aggcctgggc tggcctggca gttgaacaga gggagagact     1680

aaccttgagt cagaaaacag agaaagggta atttcctttg cttcaaattc aaggccttcc     1740

aacgccccca tcccctttac tatcattctc agtgggactc tgatcccata ttcttaacag     1800

atctttactc ttgagaaatg aataagcttt ctctcagaaa tgctgtccct atacactaga     1860

caaaactgag cctgtataag gaataaatgg gagcgccgaa aagctcccta aaaagcaa       1918


<210>  4
<211>  353
<212>  PRT
<213>  human

<400>  4

Met Glu Leu Thr Glu Leu Leu Leu Val Val Met Leu Leu Leu Thr Ala 
1               5                   10                  15      


Arg Leu Thr Leu Ser Ser Pro Ala Pro Pro Ala Cys Asp Leu Arg Val 
            20                  25                  30          


Leu Ser Lys Leu Leu Arg Asp Ser His Val Leu His Ser Arg Leu Ser 
        35                  40                  45              


Gln Cys Pro Glu Val His Pro Leu Pro Thr Pro Val Leu Leu Pro Ala 
    50                  55                  60                  


Val Asp Phe Ser Leu Gly Glu Trp Lys Thr Gln Met Glu Glu Thr Lys 
65                  70                  75                  80  


Ala Gln Asp Ile Leu Gly Ala Val Thr Leu Leu Leu Glu Gly Val Met 
                85                  90                  95      


Ala Ala Arg Gly Gln Leu Gly Pro Thr Cys Leu Ser Ser Leu Leu Gly 
            100                 105                 110         


Gln Leu Ser Gly Gln Val Arg Leu Leu Leu Gly Ala Leu Gln Ser Leu 
        115                 120                 125             


Leu Gly Thr Gln Leu Pro Pro Gln Gly Arg Thr Thr Ala His Lys Asp 
    130                 135                 140                 


Pro Asn Ala Ile Phe Leu Ser Phe Gln His Leu Leu Arg Gly Lys Val 
145                 150                 155                 160 


Arg Phe Leu Met Leu Val Gly Gly Ser Thr Leu Cys Val Arg Arg Ala 
                165                 170                 175     


Pro Pro Thr Thr Ala Val Pro Ser Arg Thr Ser Leu Val Leu Thr Leu 
            180                 185                 190         


Asn Glu Leu Pro Asn Arg Thr Ser Gly Leu Leu Glu Thr Asn Phe Thr 
        195                 200                 205             


Ala Ser Ala Arg Thr Thr Gly Ser Gly Leu Leu Lys Trp Gln Gln Gly 
    210                 215                 220                 


Phe Arg Ala Lys Ile Pro Gly Leu Leu Asn Gln Thr Ser Arg Ser Leu 
225                 230                 235                 240 


Asp Gln Ile Pro Gly Tyr Leu Asn Arg Ile His Glu Leu Leu Asn Gly 
                245                 250                 255     


Thr Arg Gly Leu Phe Pro Gly Pro Ser Arg Arg Thr Leu Gly Ala Pro 
            260                 265                 270         


Asp Ile Ser Ser Gly Thr Ser Asp Thr Gly Ser Leu Pro Pro Asn Leu 
        275                 280                 285             


Gln Pro Gly Tyr Ser Pro Ser Pro Thr His Pro Pro Thr Gly Gln Tyr 
    290                 295                 300                 


Thr Leu Phe Pro Leu Pro Pro Thr Leu Pro Thr Pro Val Val Gln Leu 
305                 310                 315                 320 


His Pro Leu Leu Pro Asp Pro Ser Ala Pro Thr Pro Thr Pro Thr Ser 
                325                 330                 335     


Pro Leu Leu Asn Thr Ser Tyr Thr His Ser Gln Asn Leu Ser Gln Glu 
            340                 345                 350         


Gly 
    


<210>  5
<211>  2259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The mRNA sequence transcribed from the humanized THPO gene

<400>  5
gaggaggggc tgtaccccaa ccagtgtctt cctacctact tgttccccag agtgctgcct       60

gctgtgcacc tgggtcctga ggcccttctc cacccggaca gagtccttgg cccacctctc      120

tcccacccga ctctgccgaa agaagcacag aagctcaagc cgcctccatg gccccaggaa      180

agattcaggg gagaggcccc atacagggag ccacttcagt tagacaccct ggccagaatg      240

gagctgactg aattgctcct cgtggtcatg cttctcctaa ctgcaaggct aacgctgtcc      300

agcccggctc ctcctgcttg tgacctccga gtcctcagta aactgcttcg tgactcccat      360

gtccttcaca gcagactgag ccagtgccca gaggttcacc ctttgcctac acctgtcctg      420

ctgcctgctg tggactttag cttgggagaa tggaaaaccc agatggagga gaccaaggca      480

caggacattc tgggagcagt gacccttctg ctggagggag tgatggcagc acggggacaa      540

ctgggaccca cttgcctctc atccctcctg gggcagcttt ctggacaggt ccgtctcctc      600

cttggggccc tgcagagcct ccttggaacc cagcttcctc cacagggcag gaccacagct      660

cacaaggatc ccaatgccat cttcctgagc ttccaacacc tgctccgagg aaaggtgcgt      720

ttcctgatgc ttgtaggagg gtccaccctc tgcgtcaggc gggccccacc caccacagct      780

gtccccagca gaacctctct agtcctcaca ctgaacgagc tcccaaacag gacttctgga      840

ttgttggaga caaacttcac tgcctcagcc agaactactg gctctgggct tctgaagtgg      900

cagcagggat tcagagccaa gattcctggt ctgctgaacc aaacctccag gtccctggac      960

caaatccccg gatacctgaa caggatacac gaactcttga atggaactcg tggactcttt     1020

cctggaccct cacgcaggac cctaggagcc ccggacattt cctcaggaac atcagacaca     1080

ggctccctgc cacccaacct ccagcctgga tattctcctt ccccaaccca tcctcctact     1140

ggacagtata cgctcttccc tcttccaccc accttgccca cccctgtggt ccagctccac     1200

cccctgcttc ctgacccttc tgctccaacg cccaccccta ccagccctct tctaaacaca     1260

tcctacaccc actcccagaa tctgtctcag gaagggtagc atatgcgcgg gcactggccc     1320

agtgagcgtc tgcagcttct ctcggggaca agcttcccca ggaaggctga gaggcagctg     1380

catctgctcc agatgttctg ctttcaccta aaaggccctg gggaagggat acacagcact     1440

ggagattgta aaattttagg agctattttt ttttaaccta tcagcaatat tcatcagagc     1500

agctagcgat ctttggtcta ttttcggtat aaatttgaaa atcactaatt ctctatatgt     1560

gctttcatac attaagtctg caaatgccta ggcaggtctt gccttttaac ccaggtagat     1620

gctacaccat atcagaaaac aaaagggaac tttcctttgc ttcaagttta agccttcccg     1680

cgcctccaac acccccacaa tgccctcctt cccttcactg taattctcag tgagactgta     1740

tggtcctcga gatatactgc tcttgataaa gaattaacag gctatcactt agaaagactg     1800

tccctactta agagacaaaa ctgaacctgt aagagaataa ctgggagcac ccaaaagcca     1860

ataaaaatca gggaccaatg ttcttcactg gggcaacaga gctcctggac cctgcctccc     1920

aagaaagcta acaggaagcc tgggagctcc acaccccagg taaggctgtg cagctggctc     1980

agtaaagagc agacttggat gtggcagctg agcaaagagc atcagcagct cagcaggggc     2040

tcagccaggc ctgggctcct gcttccctcc tgtggaggtc aggcggaagt gcaggaagtg     2100

gcaagaggca ggctcctcgg ctcacacagc aggacaagca cagagcgctt gaaggctctt     2160

tataatttcc acaaatgcac ctaaaaagca gccctgtgtg gccactccaa actttattgg     2220

aaccctccca aatgaaggca gacttatagg actttccaa                            2259


<210>  6
<211>  1309
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The DNA sequence of the humanized THPO gene

<400>  6
ttccttgaaa cctgatgaac gattcactat ttctgtcatt ttcaggacag agtccttggc       60

ccacctctct cccacccgac tctgccgaaa gaagcacaga agctcaagcc gcctccatgg      120

ccccaggaaa gattcagggg agaggcccca tacagggagc cacttcagtt agacaccctg      180

gccagaatgg agctgactga attgctcctc gtggtcatgc ttctcctaac tgcaaggcta      240

acgctgtcca gcccggctcc tcctgcttgt gacctccgag tcctcagtaa actgcttcgt      300

gactcccatg tccttcacag cagactgagc cagtgcccag aggttcaccc tttgcctaca      360

cctgtcctgc tgcctgctgt ggactttagc ttgggagaat ggaaaaccca gatggaggag      420

accaaggcac aggacattct gggagcagtg acccttctgc tggagggagt gatggcagca      480

cggggacaac tgggacccac ttgcctctca tccctcctgg ggcagctttc tggacaggtc      540

cgtctcctcc ttggggccct gcagagcctc cttggaaccc agcttcctcc acagggcagg      600

accacagctc acaaggatcc caatgccatc ttcctgagct tccaacacct gctccgagga      660

aaggtgcgtt tcctgatgct tgtaggaggg tccaccctct gcgtcaggcg ggccccaccc      720

accacagctg tccccagcag aacctctcta gtcctcacac tgaacgagct cccaaacagg      780

acttctggat tgttggagac aaacttcact gcctcagcca gaactactgg ctctgggctt      840

ctgaagtggc agcagggatt cagagccaag attcctggtc tgctgaacca aacctccagg      900

tccctggacc aaatccccgg atacctgaac aggatacacg aactcttgaa tggaactcgt      960

ggactctttc ctggaccctc acgcaggacc ctaggagccc cggacatttc ctcaggaaca     1020

tcagacacag gctccctgcc acccaacctc cagcctggat attctccttc cccaacccat     1080

cctcctactg gacagtatac gctcttccct cttccaccca ccttgcccac ccctgtggtc     1140

cagctccacc ccctgcttcc tgacccttct gctccaacgc ccacccctac cagccctctt     1200

ctaaacacat cctacaccca ctcccagaat ctgtctcagg aagggtagca tatgcgcggg     1260

cactggccca gtgagcgtct gcagcttctc tcggggacaa gcttcccca                 1309


<210>  7
<211>  1419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5 homologous arm

<400>  7
gcaagcacca tttggggtta agtgggggta tggtatgaag aacaaaaaag aaccgggctc       60

ttagctgggg gaggggcaag caaactggaa cctacaggca ctgacctttg tcaagaagag      120

tggagccttc ccagaatggg aggagcagga cagagcaggg aaagggggtt gggtagctgg      180

tttatgaggg attattcaca gactcagaat tcagcatagg tctggctgct ttggggccat      240

gagaaaataa gaaagggggt gcgtgcaggc ctggagaact ttgtccagag gaacgtaatc      300

ctaattccca ccctcatatg gtcattcttg ttagcttaag gttctgagtc tggcactggg      360

aagctgggtc aggtgagcca aggcacggaa agagggtaac aggagggggg cgtgtgtctg      420

ttgacagacc tacaggagat cccaatattg aatcaggtgc aggtctcggc tcaacttgtg      480

aaaagaggag ggtcctaatg cctctttgct caacttgtga aaagcgaggg tcccaggaag      540

ggcctggaag ggatctggag ggggcaggga ggcagatgca gttgccgtaa aaaaacgctg      600

gcagtgtcta aggtgaagga agccatgtga ggcagatatc atcttgctct gtaagtgcca      660

cccaggtctc tggcctcagg aaagaagtcc tagttctatt acccactttg acttgagtac      720

ttgaaaatcc aaagaaaagt cctgggtttt atttgtgggc caaaagggaa agaatgtcca      780

agtttctctt ttaattcctg cctctggtgg tctgttatgt ccttacccag tctgcctcag      840

tcccactccc aacaccgttc cttagtgcat gtgtccattt gcctgcccat ttgctttttc      900

ttcttggcct ggtgacccat tttccactct tctccaaact taacaacacc cctctctatt      960

cccgtcccac attctctccc agctaacaac caatgctcag aatttgagtc cacttaaaat     1020

gtgtgcgtaa catctcgctg ctcttagcag ggcaacaatt ggtagatggc accaagatct     1080

tatcagagtc actgaagagc tgttacaaga ggaggcacta taatgcttca gtcacaagaa     1140

aatatgggac cagttggaac acaaacagct ctggccctag ctggccagca gttcaaggcc     1200

tccaagcacc cgtttcccgt gccagaaagt cttttccttg aaacctgatg aacgattcac     1260

tatttctgtc attttcagga cagagtcctt ggcccacctc tctcccaccc gactctgccg     1320

aaagaagcac agaagctcaa gccgcctcca tggccccagg aaagattcag gggagaggcc     1380

ccatacaggg agccacttca gttagacacc ctggccaga                            1419


<210>  8
<211>  1645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3 homologous arm

<400>  8
tagcatatgc gcgggcactg gcccagtgag cgtctgcagc ttctctcggg gacaagcttc       60

cccaggaagg ctgagaggca gctgcatctg ctccagatgt tctgctttca cctaaaaggc      120

cctggggaag ggatacacag cactggagat tgtaaaattt taggagctat ttttttttaa      180

cctatcagca atattcatca gagcagctag cgatctttgg tctattttcg gtataaattt      240

gaaaatcact aattctctat atgtgctttc atacattaag tctgcaaatg cctaggcagg      300

tcttgccttt taacccaggt agatgctaca ccatatcaga aaacaaaagg gaactttcct      360

ttgcttcaag tttaagcctt cccgcgcctc caacaccccc acaatgccct ccttcccttc      420

actgtaattc tcagtgagac tgtatggtcc tcgagatata ctgctcttga taaagaatta      480

acaggctatc acttagaaag actgtcccta cttaagagac aaaactgaac ctgtaagaga      540

ataactggga gcacccaaaa gccaataaaa atcagggacc aatgttcttc actggggcaa      600

cagagctcct ggaccctgcc tcccaagaaa gctaacagga agcctgggag ctccacaccc      660

caggtaaggc tgtgcagctg gctcagtaaa gagcagactt ggatgtggca gctgagcaaa      720

gagcatcagc agctcagcag gggctcagcc aggcctgggc tcctgcttcc ctcctgtgga      780

ggtcaggcgg aagtgcagga agtggcaaga ggcaggctcc tcggctcaca cagcaggaca      840

agcacagagc gcttgaaggc tctttataat ttccacaaat gcacctaaaa agcagccctg      900

tgtggccact ccaaacttta ttggaaccct cccaaatgaa ggcagactta taggactttc      960

caagtaaacc cgagtctcag ctttgttggt gctagaggtt tacattgcgc aagaacaaaa     1020

actgtattgc caagggtcaa cctgcaatag ccagagtctt atgaaaagac tggtgagagc     1080

ttttccctct cttgtgtccc aaagtaaagg aggaaagacc accatgtagg tggaaaagcc     1140

aggatctcac agttccaaac acacacacac acacgtgtgg ggatgacaat tcagacagtg     1200

ccaggtgtct acacaggcag aacctaatga acggatcaac agatgcacat agctaaaact     1260

gttatcagaa tctcagtgtc agtttctatg tgacagagac cttcatcttc taattctagg     1320

ataaatatac tttgtaattt gtcgtaacac taaaagaggc taaatttaac atttaaaaag     1380

gaatttaggg ccagtgagat ggctcagtag ttgaacgtgt tttgttttgt gccagtctga     1440

cattaatctg atgtctggat cctatggaaa aggcaagaac tcccagaagt tgtcttccaa     1500

cttcattatg cacaccatca catgccaact cccacacttg cacatacaca tcacacatac     1560

acacatgata ataaaataaa atttaagtta aaaaaaaaaa aagctggaca taatggcaca     1620

tgcctgtaaa acccggcatt tcagc                                           1645


<210>  9
<211>  1059
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a nucleic acid sequence that is inserted between the 5 homologous arm and the 3 homologous arm

<400>  9
atggagctga ctgaattgct cctcgtggtc atgcttctcc taactgcaag gctaacgctg       60

tccagcccgg ctcctcctgc ttgtgacctc cgagtcctca gtaaactgct tcgtgactcc      120

catgtccttc acagcagact gagccagtgc ccagaggttc accctttgcc tacacctgtc      180

ctgctgcctg ctgtggactt tagcttggga gaatggaaaa cccagatgga ggagaccaag      240

gcacaggaca ttctgggagc agtgaccctt ctgctggagg gagtgatggc agcacgggga      300

caactgggac ccacttgcct ctcatccctc ctggggcagc tttctggaca ggtccgtctc      360

ctccttgggg ccctgcagag cctccttgga acccagcttc ctccacaggg caggaccaca      420

gctcacaagg atcccaatgc catcttcctg agcttccaac acctgctccg aggaaaggtg      480

cgtttcctga tgcttgtagg agggtccacc ctctgcgtca ggcgggcccc acccaccaca      540

gctgtcccca gcagaacctc tctagtcctc acactgaacg agctcccaaa caggacttct      600

ggattgttgg agacaaactt cactgcctca gccagaacta ctggctctgg gcttctgaag      660

tggcagcagg gattcagagc caagattcct ggtctgctga accaaacctc caggtccctg      720

gaccaaatcc ccggatacct gaacaggata cacgaactct tgaatggaac tcgtggactc      780

tttcctggac cctcacgcag gaccctagga gccccggaca tttcctcagg aacatcagac      840

acaggctccc tgccacccaa cctccagcct ggatattctc cttccccaac ccatcctcct      900

actggacagt atacgctctt ccctcttcca cccaccttgc ccacccctgt ggtccagctc      960

caccccctgc ttcctgaccc ttctgctcca acgcccaccc ctaccagccc tcttctaaac     1020

acatcctaca cccactccca gaatctgtct caggaaggg                            1059


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  10
cagaatggag ctgactggta agg                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  11
taaggctaca tgaagggcta ggg                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  12
gctagggaca agttcaagaa tgg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  13
ggacaagttc aagaatggct tgg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  14
ttcaagaatg gcttggccgc agg                                               23


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  15
gaatggcttg gccgcagggc agg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  16
ggcttggccg cagggcaggt ggg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  17
tgtttcctga gacaaattcc tgg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  18
tcatcccagg aatttgtctc agg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  19
ggatgagggt acattgtgac tgg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  20
ggatgagggg cggtagagtt agg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  21
ggcggtagag ttaggcatgg tgg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  22
gagttaggca tggtggtgga agg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  23
ggcatggtgg tggaagggtc agg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  24
gagacaaatt cctgggatga ggg                                               23


<210>  25
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA4 Upstream

<400>  25
acaagttcaa gaatggct                                                     18


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA4 Upstream

<400>  26
taggacaagt tcaagaatgg ct                                                22


<210>  27
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA4 Downstream

<400>  27
agccattctt gaacttgt                                                     18


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA4 Downstream

<400>  28
aaacagccat tcttgaactt gt                                                22


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA15 Upstream

<400>  29
agacaaattc ctgggatga                                                    19


<210>  30
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA15 Upstream

<400>  30
taggagacaa attcctggga tga                                               23


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA15 Downstream

<400>  31
tcatcccagg aatttgtct                                                    19


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA15 Downstream

<400>  32
aaactcatcc caggaatttg tct                                               23


<210>  33
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a DNA fragment containing the T7 promoter and sgRNA scaffold

<400>  33
gaattctaat acgactcact atagggggtc ttcgagaaga cctgttttag agctagaaat       60

agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct      120

tttaaaggat cc                                                          132


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  34
atgctcacca gatggctcag                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  35
ctgggtttgt cacaggagct                                                   20


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  36
gctgactgaa ttgctcctcg tg                                                22


<210>  37
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe

<400>  37
ccaaggagga gacggacctg tcc                                               23


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
tgggcaggct tgtgacccta ctac                                              24


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
ccagggacct ggaggtttgg tt                                                22


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
cacagctgtc cccagcagaa cc                                                22


<210>  41
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  41
ggctgcctgg gactttgtca gtgc                                              24


<210>  42
<211>  326
<212>  PRT
<213>  rat

<400>  42

Met Glu Leu Thr Asp Leu Leu Leu Val Ala Ile Leu Leu Leu Thr Ala 
1               5                   10                  15      


Arg Leu Thr Leu Ser Ser Pro Val Pro Pro Ala Cys Asp Pro Arg Leu 
            20                  25                  30          


Leu Asn Lys Leu Leu Arg Asp Ser Tyr Leu Leu His Ser Arg Leu Ser 
        35                  40                  45              


Gln Cys Pro Asp Val Asn Pro Leu Ser Ile Pro Val Leu Leu Pro Ala 
    50                  55                  60                  


Val Asp Phe Ser Leu Gly Glu Trp Lys Thr Gln Thr Glu Gln Ser Lys 
65                  70                  75                  80  


Ala Gln Asp Ile Leu Gly Ala Val Ser Leu Leu Leu Glu Gly Val Met 
                85                  90                  95      


Ala Ala Arg Gly Gln Leu Glu Pro Ser Cys Leu Ser Ser Leu Leu Gly 
            100                 105                 110         


Gln Leu Ser Gly Gln Val Arg Leu Leu Leu Gly Ala Leu Gln Gly Leu 
        115                 120                 125             


Leu Gly Thr Gln Leu Pro Pro Gln Gly Arg Thr Thr Ala His Lys Asp 
    130                 135                 140                 


Pro Ser Ala Leu Phe Leu Ser Leu Gln Gln Leu Leu Arg Gly Lys Val 
145                 150                 155                 160 


Arg Phe Leu Leu Leu Val Glu Gly Pro Ala Leu Cys Val Arg Arg Thr 
                165                 170                 175     


Leu Pro Thr Thr Ala Val Pro Ser Arg Thr Ser Gln Leu Leu Thr Leu 
            180                 185                 190         


Asn Lys Phe Pro Asn Arg Thr Ser Gly Leu Leu Glu Thr Asn Phe Ser 
        195                 200                 205             


Val Val Ala Arg Thr Ala Gly Pro Gly Leu Leu Asn Arg Leu Gln Gly 
    210                 215                 220                 


Phe Arg Ala Lys Ile Ile Pro Gly Gln Leu Asn Gln Thr Ser Gly Ser 
225                 230                 235                 240 


Leu Asp Gln Ile Pro Gly Tyr Leu Asn Gly Thr His Glu Pro Val Asn 
                245                 250                 255     


Gly Thr His Gly Leu Phe Ala Gly Thr Ser Leu Gln Thr Leu Glu Ala 
            260                 265                 270         


Pro Asp Val Val Pro Gly Ala Phe Asn Lys Gly Ser Leu Pro Leu Asn 
        275                 280                 285             


Leu Gln Ser Gly Leu Pro Pro Ile Pro Ser Leu Ala Ala Asp Gly Tyr 
    290                 295                 300                 


Thr Leu Phe Pro Pro Ser Pro Thr Phe Pro Thr Pro Gly Ser Pro Pro 
305                 310                 315                 320 


Gln Leu Pro Pro Val Ser 
                325     
