б

<110>ϾƼ޹˾

<120>һֻٲĻ༭

<130>IP14

<160>10

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>˹

<220>
<223>TRAC sgRNAб

<400>  1

agagtctctcagctggtacacgg23


<210> 2
<211>813
<212> DNA
<213>˹

<220>
<223>һͬԴ

<400>  2

ggccatattc tggcagggtc agtggctcca actaacattt gtttggtact ttacagttta   60
ttaaatagat gtttatatgg agaagctctc atttctttctcagaagagcctggctaggaa  120
ggtggatgaggcaccatattcattttgcaggtgaaattcctgagatgtaaggagctgctg  180
tgacttgctcaaggccttatatcgagtaaacggtagtgctggggcttagacgcaggtgtt240
ctgatttatagttcaaaacctctatcaatgagagagcaatctcctggtaatgtgatagat  300
ttcccaacttaatgccaacataccataaacctcccattctgctaatgcccagcctaagtt 360
ggggagaccactccagattccaagatgtacagtttgctttgctgggcctttttcccatgc 420 
ctgcctttactctgccagagttatattgctggggttttgaagaagatcctattaaataaa 480
agaataagcagtattattaagtagccctgcatttcaggtttccttgagtggcaggccagg 540
cctggccgtgaacgttcactgaaatcatggcctcttggccaagattgatagcttgtgcct 600
gtccctgagtcccagtccatcacgagcagctggtttctaagatgctatttcccgtataaa 660
gcatgagaccgtgacttgccagccccacagagccccgcccttgtccatcactggcatctg 720
gactccagcctgggttggggcaaagagggaaatgagatcatgtcctaaccctgatcctct 780
tgtcccacagatatccagaaccctgaccctgcc      813


<210> 3
<211> 2818
<212> DNA
<213>˹

<220>
<223>CD19-CD22-CAR

<400>  3

gtgaaacagactttgaattttgaccttctcaagttggcgggagacgtggagtccaaccca    60
gggccgtctagactgctgctcgtgacaagcctgctgctgtgcgagctgccccaccctgcc   120
tttctgctgatccccgacatccagatgacccagaccaccagcagcctgagcgccagcctg   180
ggcgatagagtgaccatcagctgcagagccagccaggacatcagcaagtacctgaactgg   240
tatcagcagaaacccgacggcaccgtgaagctgctgatctaccacaccagcagactgcac   300
agcggcgtgcccagcagattttctggcagcggctccggcaccgactacagcctgaccatc   360
tccaacctggaacaggaagatatcgctacctacttctgtcagcaaggcaacaccctgccc   420
tacaccttcggcggaggcaccaagctggaaatcacaggcggcggaggatcccaggtgcag   480
ctgcagcagtctggacccggcctcgtgaagcctagccagaccctgtctctgacctgcgcc   540
atcagcggcgatagcgtgtccagcaatagcgccgcctggaactggatccggcagagccct   600
tctagaggcctggaatggctgggccggacctactaccggtccaagtggtacaacgactac   660
gccgtgtccgtgaagtcccggatcaccatcaaccccgacaccagcaagaaccagttctcc   720
ctgcagctgaacagcgtgacccccgaggataccgccgtgtactactgcgccagagaagtg   780
accggcgacctggaagatgccttcgacatctggggccagggcacaatggtcaccgtgtct   840
agcggcagcacaagcggctctggcaagcctggatctggcgagggctctaccaagggcgat   900 
attcagatgacacagagcccctccagcctgtccgcctctgtgggagacagagtgacaatc   960
acctgtcgggcctcccagaccatctggtcctatctgaattggtatcagcagcggcctggc  1020
aaggcccccaacctgctgatctatgccgccagctctctgcagtccggcgtgccatctaga  1080
ttcagcggcagaggcagcggcaccgatttcaccctgacaattagcagtctgcaggccgag  1140
gacttcgccacctactattgccagcagagctacagcatcccccagaccttcggccaggga  1200
acaaaactggaaatcaaagggggaggcggcagcgaagtgaaactgcaggaatctggccct  1260
ggcctggtggccccaagccagtctctgagcgtgacctgtaccgtgtctggcgtgtccctg  1320
cccgattacggcgtgtcctggatcagacagccccccagaaagggactggaatggctggga  1380
gtgatctggggcagcgagacaacctactacaacagcgccctgaagtccaggctgaccatc  1440
atcaaggacaactccaagagccaggtgttcctgaagatgaattccctgcagaccgacgac  1500
accgccatctattactgtgccaagcactactactacggcggcagctacgccatggactac  1560
tggggacagggaacctccgtgaccgtgtcctcttccggaaccacgacgccagcgccgcga  1620
ccaccaacaccggcgcccaccatcgcgtcgcagcccctgtccctgcgcccagaggcgtgc  1680
cggccagcggcggggggcgcagtgcacacgagggggctggacttcgcctgtgatatctac  1740
atctgggcgcccttggccgggacttgtggggtccttctcctgtcactggttatcaccctt  1800
tactgcaaacggggcagaaagaaactcctgtatatattcaaacaaccatttatgagacca  1860
gtacaaactactcaagaggaagatggctgtagctgccgatttccagaagaagaagaagga  1920
ggatgtgaactgagagtgaagttcagcaggagcgcagacgcccccgcgtacaagcagggc  1980
cagaaccagctctataacgagctcaatctaggacgaagagaggagtacgatgttttggac  2040
aagagacgtggccgggaccctgagatggggggaaagccgagaaggaagaaccctcaggaa  2100
ggcctgtacaatgaactgcagaaagataagatggcggaggcctacagtgagattgggatg  2160
aaaggcgagcgccggaggggcaaggggcacgatggcctttaccagggtctcagtacagcc  2220
accaaggacacctacgacgcccttcacatgcaggccctgccccctcgctaagtcgacctg  2280
cagaagcttgcctcgagcagcgctgctcgagagatctacgggtggcatccctgtgacccc  2340
tccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtccta  2400
ataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtgg  2460
aggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtc  2520
tattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcc  2580
tgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatg  2640
accaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccagg  2700
ctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctggga  2760
ttacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggaaggtaacc    2818



<210> 4
<211> 693
<212> DNA
<213>˹

<220>
<223>ڶͬԴ

<400>  4

aaatccagtgacaagtctgtctgcctattcaccgattttgattctcaaacaaatgtgtca     60
caaagtaaggattctgatgtgtatatcacagacaaaactgtgctagacatgaggtctatg    120
gacttcaagagcaacagtgctgtggcctggagcaacaaatctgactttgcatgtgcaaac    180
gccttcaacaacagcattattccagaagacaccttcttccccagcccaggtaagggcagc    240
tttggtgccttcgcaggctgtttccttgcttcaggaatggccaggttctgcccagagctc    300
tggtcaatgatgtctaaaactcctctgattggtggtctcggccttatccattgccaccaa    360
aaccctctttttactaagaaacagtgagccttgttctggcagtccagagaatgacacggg    420
aaaaaagcagatgaagagaaggtggcaggagagggcacgtggcccagcctcagtctctcc    480
aactgagttcctgcctgcctgcctttgctcagactgtttgccccttactgctcttctagg    540
cctcattctaagccccttctccaagttgcctctccttatttctccctgtctgccaaaaaa    600
tctttcccagctcactaagtcagtctcacgcagtcactcattaacccaccaatcactgat    660
tgtgccggcacatgaatgcaccaggtgttgaag                                693


<210> 5
<211>97
<212> DNA
<213>˹

<220>
<223>TRACsgRNA

<400> 5

agagtctctcagctggtacagttttagagctagaaatagcaagttaaaataaggctagtc     60
cgttatcaacttgaaaaagtggcaccgagtcggtgct                            97


<210> 6
<211>4242
<212> DNA
<213>˹

<220>
<223>Cas9 mRNA

<400> 6

atggcgtatccttatgacgtgcctgactatgccagcctgatggccccaaagaagaagcgg    60
aaggtcggtatccacggagtcccagcagccgacaagaagtacagcatcggcctggacatc   120
ggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaa   180
ttcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctg   240
ctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaaga   300
tacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggcc   360
aaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataag   420
aagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaag   480
taccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctg   540
cggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgag   600
ggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacc   660
tacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatc   720
ctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggc   780
gagaagaagaatggcctgttcggaaacctgattgccctgagcctgggcctgacccccaac   840
ttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctac   900
gacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctg   960
gccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgag  1020
atcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggac  1080
ctgaccctgctgaaagctctcgtgcggcagcagctgcctgagaagtacaaagagattttc  1140
ttcgaccagagcaagaacggctacgccggctacattgacggcggagccagccaggaagag  1200
ttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtg  1260
aagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccc  1320
caccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttaccca  1380
ttcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactac  1440
gtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaa  1500
accatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttc  1560
atcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcac  1620
agcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaatacgtgacc  1680
gagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctg  1740
ctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaa  1800
atcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctg  1860
ggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaa  1920
aacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatg  1980
atcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctg  2040
aagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgg  2100
gacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacaga  2160
aacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcc  2220
caggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagcccc  2280
gccattaagaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatg  2340
ggccggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccacccag  2400
aagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctg  2460
ggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacgagaagctg  2520
tacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactggacatcaac  2580
cggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactcc  2640
atcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccc  2700
tccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctg  2760
attacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactg  2820
gataaggccggcttcatcaagagacagctggtggaaacccggcagatcacaaagcacgtg  2880
gcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgg  2940
gaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccag  3000
ttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgcc  3060  
gtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggc  3120
gactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaag  3180
gctaccgccaagtacttcttctacagcaacatcatgaactttttcaagaccgagattacc  3240
ctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggag  3300
atcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaa  3360
gtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctg  3420
cccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtac  3480
ggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaag  3540
ggcaagtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaaga  3600
agcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaa  3660
aaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaag  3720  
agaatgctggcctctgccggcgaactgcagaagggaaacgaactggccctgccctccaaa  3780
tatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggat  3840
aatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgag  3900
cagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctg  3960
tccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccac  4020
ctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatc  4080
gaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgatccaccagagc  4140
atcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgacaaaaggccg  4200
gcggccacgaaaaaggccggccaggcaaaaaagaaaaagtaa                    4242


<210>7
<211>25
<212> DNA
<213>˹

<220>
<223>PCR-F

<400>7

ctcagcactaaggaaaagcctccag                 25


<210>8
<211>25
<212> DNA
<213>˹

<220>
<223>PCR-R

<400>8

cgccaacttgagaaggtcaaaattc                  25


<210>9
<211>22
<212>  DNA
<213>˹

<220>
<223>sgRNA-4б

<400>9

gcgctgcttcgcgatgtacggg                     22



<210>10
<211>103
<212> DNA
<213>˹

<220>
<223>sgRNA-4беsgRNA

<400>10

gggcgctgcttcgcgatgtagttttagagctagaaatagcaagttaaaataaggctagtc     60
cgttatcaacttgaaaaagtggcaccgagtcggtgcttttttt                      103

