                         SEQUENCE LISTING

<110>  University of Utah Research Foundation
 
<120>  NOVEL MINI-INSULIN WITH EXTENDED C-TERMINAL A CHAIN

<130>  21101.0404P1

<140>  PCT/US2020/057078
<141>  2020-10-03

<150>  62/925,617
<151>  2019-10-24

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  1

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  2
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  2

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  3
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin

<400>  3

Gly Val Asn Gln His Leu Cys Gly Ser Glu Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Tyr Glu Arg 
            20          


<210>  4
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  4

Phe Val Asn Gln His Leu Cys Gly Ser Glu Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Phe Glu Arg 
            20          


<210>  5
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  5

Phe Val Asn Gln His Leu Cys Gly Ser Glu Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Pro Glu Arg 
            20          


<210>  6
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  6

Phe Val Asn Gln His Leu Cys Gly Ser Asp Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Tyr Glu Arg 
            20          


<210>  7
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  7

Phe Val Asn Gln His Leu Cys Gly Ser Asp Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Phe Glu Arg 
            20          


<210>  8
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  8

Phe Val Asn Gln His Leu Cys Gly Ser Asp Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Pro Glu Arg 
            20          


<210>  9
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  9

Phe Val Asn Gln His Leu Cys Gly Ser Gln Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Tyr Glu Arg 
            20          


<210>  10
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  10

Phe Val Asn Gln His Leu Cys Gly Ser Gln Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Phe Glu Arg 
            20          


<210>  11
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  11

Phe Val Asn Gln His Leu Cys Gly Ser Gln Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Pro Glu Arg 
            20          


<210>  12
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  12

Gly Ile Val Glu Gln Cys Cys His Arg Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  13
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  13

Gly Ile Val Glu Gln Cys Cys Tyr Arg Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  14
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  14

Gly Ile Val Glu Gln Cys Cys Lys Arg Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  15
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  gamma-carboxyglutamate

<400>  15

Gly Val Val Glu His Cys Cys His Arg Pro Cys Ser Asn Ala Glu Phe 
1               5                   10                  15      


Lys Lys Tyr Cys 
            20  


<210>  16
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  hydroxyproline

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  gamma-carboxyglutamate

<400>  16

Thr Phe Asp Thr Pro Lys His Arg Cys Gly Ser Glu Ile Thr Asn Ser 
1               5                   10                  15      


Tyr Met Asp Leu Cys Tyr Arg 
            20              


<210>  17
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  17

Phe Asn Val Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg 
            20          


<210>  18
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  18

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Tyr Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg 
            20          


<210>  19
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  19

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Tyr Glu Arg 
            20          


<210>  20
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  20

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Tyr Tyr 
1               5                   10                  15      


Leu Val Cys Tyr Glu Arg 
            20          


<210>  21
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain


<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Xaa can be any naturally occurring amino acid

<400>  21

Phe Val Asn Gln His Leu Cys Gly Ser Glu Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Xaa Glu Arg 
            20          


<210>  22
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  22

Gly Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  23
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  23

Phe Val Asn Gln His Leu Cys Gly Ser Glu Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg 
            20          


<210>  24
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  24

Gly Ile Val Glu Gln Cys Cys Thr Arg Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  25
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  gamma-carboxyglutamate

<400>  25

Gly Val Val Glu His Cys Cys Tyr Arg Pro Cys Ser Asn Ala Glu Phe 
1               5                   10                  15      


Lys Lys Phe Cys 
            20  


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  gamma-carboxyglutamate

<400>  26

Gly Val Val Glu His Cys Cys Tyr Arg Pro Cys Ser Asn Ala Glu Phe 
1               5                   10                  15      


Lys Lys Phe Cys 
            20  


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  Xaa can be any amino acid

<400>  27

Xaa Xaa Xaa Xaa 
1               


<210>  28
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain


<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  gamma-carboxyglutamate

<400>  28

Asn Ser Asp Thr Pro Lys Tyr Arg Cys Gly Ser Glu Ile Pro Asn Ser 
1               5                   10                  15      


Tyr Ile Asp Leu Cys Phe 
            20          


<210>  29
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  29

Asn Ser Asp Thr Pro Lys Tyr Arg Cys Gly Ser Asp Ile Pro Asn Ser 
1               5                   10                  15      


Tyr Met Asp Leu Cys Phe 
            20          


<210>  30
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  gamma-carboxyglutamate

<400>  30

Asn Ser Asp Thr Pro Trp Asn Arg Cys Gly Ser Gln Ile Thr Asp Ser 
1               5                   10                  15      


Tyr Arg Glu Leu Cys Pro His 
            20              


<210>  31
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  31

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys His Ala Leu Gln 
            20                  


<210>  32
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  32

Gly Ile Val Glu Gln Cys Cys His Arg Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys His Ala Leu Gln 
            20                  


<210>  33
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  33

Gly Ile Val Glu Gln Cys Cys Tyr Arg Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys His Ala Leu Gln 
            20                  


<210>  34
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  34

Gly Ile Val Glu Gln Cys Cys Lys Arg Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys His Ala Leu Gln 
            20                  


<210>  35
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; mutant insulin chain

<400>  35

Phe Val Asn Gln His Leu Cys Gly Ser Glu Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Leu Glu Arg 
            20          


