                         SEQUENCE LISTING

<110>  Translate Bio, Inc.
 
<120>  COMPOSITIONS, METHODS AND USES OF MESSENGER RNA

<130>  MRT-2120WO

<150>  62/923,711
<151>  2019-10-21

<150>  62/934,842
<151>  2019-11-13

<150>  63/084,422
<151>  2020-09-28

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  636
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  1
agcgtgaaca ttagcgggca gaataccatg aacatggtca aagtgccgga atgtcgcctg       60

gccgacgaac tgggcggcct gtgggaaaac tcaaggttca cggactgctg cctttgcgtg      120

gccggccaag aattccaggc ccataaggcc atcctggccg cgcggtcgcc agtattctcg      180

gccatgttcg aacacgaaat ggaagagtct aagaagaata gagtggaaat caacgatgtg      240

gagcctgagg tctttaagga aatgatgtgc tttatataca ctggaaaggc ccccaacctc      300

gacaagatgg ccgacgactt gctggctgcc gccgacaaat acgccctgga gcggctcaag      360

gttatgtgcg aggacgcgct gtgcagcaac ctcagcgtgg agaacgccgc agaaatcctc      420

atcctggcgg atttgcactc cgccgaccaa ctcaagaccc aggccgtgga cttcattaac      480

taccacgctt ccgacgtgct ggagacttcc ggatggaagt ccatggtcgt cagccacccg      540

cacttagtgg cagaggccta cagatccctg gccagtgccc agtgcccttt cctggggccg      600

cctaggaaac gcctgaagca gagcgggggt ggctcc                                636


<210>  2
<211>  675
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  2
ggtggtggat ccggcggcgg ctccatgcct aggagagcgg agaattggga cgaagcagaa       60

gtcggagcag aagaagccgg agtggaagaa tacggacctg aagaggacgg gggagaagag      120

tcgggcgccg aagagtccgg ccccgaggag tccggacccg aagaactggg cgccgaggaa      180

gaaatggagg ccgggcgccc taggccggtg ctgcggtccg tgaactcccg cgagccgagc      240

caggtcattt tctgcaatcg cagcccgaga gtggtgctgc ccgtgtggct gaactttgac      300

ggggagcctc agccataccc taccctgcca ccgggaactg gacgcagaat ccacagctac      360

cggggccacc tttggctttt ccgggacgcc gggactcacg acgggctgct cgtgaaccag      420

accgagttgt tcgtgccgtc cctgaacgtc gatggccagc caattttcgc caacatcacc      480

ctgccggtgt acacactgaa ggaacggtgc ctccaagtcg tcagaagcct cgtcaagccc      540

gagaactacc ggcggctgga catcgtgcgg tcactctacg aagatctcga ggaccaccct      600

aacgtgcaaa aggacctgga gaggctgact caggaacgca tcgcccatca acgcatgggc      660

gacggtggtg gctcc                                                       675


<210>  3
<211>  555
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  3
ggttccggct ctggacggct gaacttcggg gacgatattc ctagcgccct gcgcatcgcc       60

aagaagaaga gatggaactc aatcgaggaa cggcgaatcc accaggagtc cgagctgcat      120

agctacctta gccgccttat cgccgcggaa cgggagaggg agctggaaga gtgtcagcgg      180

aaccatgagg gcgacgaaga tgactcccac gtccgggcac agcaggcctg catcgaggct      240

aagcacgaca agtacatggc cgatatggac gagttattca gccaagtgga cgagaagcgg      300

aagaagcgcg acatcccgga ctacttatgc ggaaagattt ccttcgaact tatgagggaa      360

ccgtgtatca ccccgtccgg gatcacctac gaccggaaag acatcgaaga acacctacag      420

cgcgtggggc acttcgaccc ggtcacccgg agcccgctga cccaagagca attaatcccc      480

aacttggcga tgaaggaagt gatcgacgcc ttcattagcg aaaatggatg ggtggaggat      540

tacgggggtg gctcc                                                       555


<210>  4
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  4
ggctggtctt gcattatact cttccttgtc gccaccgcca ctggagcgca tagc             54


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  5
tccgagaaag atgaactg                                                     18


<210>  6
<211>  212
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polypeptide

<400>  6

Ser Val Asn Ile Ser Gly Gln Asn Thr Met Asn Met Val Lys Val Pro 
1               5                   10                  15      


Glu Cys Arg Leu Ala Asp Glu Leu Gly Gly Leu Trp Glu Asn Ser Arg 
            20                  25                  30          


Phe Thr Asp Cys Cys Leu Cys Val Ala Gly Gln Glu Phe Gln Ala His 
        35                  40                  45              


Lys Ala Ile Leu Ala Ala Arg Ser Pro Val Phe Ser Ala Met Phe Glu 
    50                  55                  60                  


His Glu Met Glu Glu Ser Lys Lys Asn Arg Val Glu Ile Asn Asp Val 
65                  70                  75                  80  


Glu Pro Glu Val Phe Lys Glu Met Met Cys Phe Ile Tyr Thr Gly Lys 
                85                  90                  95      


Ala Pro Asn Leu Asp Lys Met Ala Asp Asp Leu Leu Ala Ala Ala Asp 
            100                 105                 110         


Lys Tyr Ala Leu Glu Arg Leu Lys Val Met Cys Glu Asp Ala Leu Cys 
        115                 120                 125             


Ser Asn Leu Ser Val Glu Asn Ala Ala Glu Ile Leu Ile Leu Ala Asp 
    130                 135                 140                 


Leu His Ser Ala Asp Gln Leu Lys Thr Gln Ala Val Asp Phe Ile Asn 
145                 150                 155                 160 


Tyr His Ala Ser Asp Val Leu Glu Thr Ser Gly Trp Lys Ser Met Val 
                165                 170                 175     


Val Ser His Pro His Leu Val Ala Glu Ala Tyr Arg Ser Leu Ala Ser 
            180                 185                 190         


Ala Gln Cys Pro Phe Leu Gly Pro Pro Arg Lys Arg Leu Lys Gln Ser 
        195                 200                 205             


Gly Gly Gly Ser 
    210         


<210>  7
<211>  225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polypeptide

<400>  7

Gly Gly Gly Ser Gly Gly Gly Ser Met Pro Arg Arg Ala Glu Asn Trp 
1               5                   10                  15      


Asp Glu Ala Glu Val Gly Ala Glu Glu Ala Gly Val Glu Glu Tyr Gly 
            20                  25                  30          


Pro Glu Glu Asp Gly Gly Glu Glu Ser Gly Ala Glu Glu Ser Gly Pro 
        35                  40                  45              


Glu Glu Ser Gly Pro Glu Glu Leu Gly Ala Glu Glu Glu Met Glu Ala 
    50                  55                  60                  


Gly Arg Pro Arg Pro Val Leu Arg Ser Val Asn Ser Arg Glu Pro Ser 
65                  70                  75                  80  


Gln Val Ile Phe Cys Asn Arg Ser Pro Arg Val Val Leu Pro Val Trp 
                85                  90                  95      


Leu Asn Phe Asp Gly Glu Pro Gln Pro Tyr Pro Thr Leu Pro Pro Gly 
            100                 105                 110         


Thr Gly Arg Arg Ile His Ser Tyr Arg Gly His Leu Trp Leu Phe Arg 
        115                 120                 125             


Asp Ala Gly Thr His Asp Gly Leu Leu Val Asn Gln Thr Glu Leu Phe 
    130                 135                 140                 


Val Pro Ser Leu Asn Val Asp Gly Gln Pro Ile Phe Ala Asn Ile Thr 
145                 150                 155                 160 


Leu Pro Val Tyr Thr Leu Lys Glu Arg Cys Leu Gln Val Val Arg Ser 
                165                 170                 175     


Leu Val Lys Pro Glu Asn Tyr Arg Arg Leu Asp Ile Val Arg Ser Leu 
            180                 185                 190         


Tyr Glu Asp Leu Glu Asp His Pro Asn Val Gln Lys Asp Leu Glu Arg 
        195                 200                 205             


Leu Thr Gln Glu Arg Ile Ala His Gln Arg Met Gly Asp Gly Gly Gly 
    210                 215                 220                 


Ser 
225 


<210>  8
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polypeptide

<400>  8

Gly Ser Gly Ser Gly Arg Leu Asn Phe Gly Asp Asp Ile Pro Ser Ala 
1               5                   10                  15      


Leu Arg Ile Ala Lys Lys Lys Arg Trp Asn Ser Ile Glu Glu Arg Arg 
            20                  25                  30          


Ile His Gln Glu Ser Glu Leu His Ser Tyr Leu Ser Arg Leu Ile Ala 
        35                  40                  45              


Ala Glu Arg Glu Arg Glu Leu Glu Glu Cys Gln Arg Asn His Glu Gly 
    50                  55                  60                  


Asp Glu Asp Asp Ser His Val Arg Ala Gln Gln Ala Cys Ile Glu Ala 
65                  70                  75                  80  


Lys His Asp Lys Tyr Met Ala Asp Met Asp Glu Leu Phe Ser Gln Val 
                85                  90                  95      


Asp Glu Lys Arg Lys Lys Arg Asp Ile Pro Asp Tyr Leu Cys Gly Lys 
            100                 105                 110         


Ile Ser Phe Glu Leu Met Arg Glu Pro Cys Ile Thr Pro Ser Gly Ile 
        115                 120                 125             


Thr Tyr Asp Arg Lys Asp Ile Glu Glu His Leu Gln Arg Val Gly His 
    130                 135                 140                 


Phe Asp Pro Val Thr Arg Ser Pro Leu Thr Gln Glu Gln Leu Ile Pro 
145                 150                 155                 160 


Asn Leu Ala Met Lys Glu Val Ile Asp Ala Phe Ile Ser Glu Asn Gly 
                165                 170                 175     


Trp Val Glu Asp Tyr Gly Gly Gly Ser 
            180                 185 


<210>  9
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polypeptide

<400>  9

Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly Ala 
1               5                   10                  15      


His Ser 
        


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polypeptide

<400>  10

Ser Glu Lys Asp Glu Leu 
1               5       


<210>  11
<211>  140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  11
ggacagaucg ccuggagacg ccauccacgc uguuuugacc uccauagaag acaccgggac       60

cgauccagcc uccgcggccg ggaacggugc auuggaacgc ggauuccccg ugccaagagu      120

gacucaccgu ccuugacacg                                                  140


<210>  12
<211>  105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  12
cggguggcau cccugugacc ccuccccagu gccucuccug gcccuggaag uugccacucc       60

agugcccacc agccuugucc uaauaaaauu aaguugcauc aagcu                      105


<210>  13
<211>  105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  13
ggguggcauc ccugugaccc cuccccagug ccucuccugg cccuggaagu ugccacucca       60

gugcccacca gccuuguccu aauaaaauua aguugcauca aagcu                      105


<210>  14
<211>  874
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic polypeptide

<400>  14

Met Gln Asp Leu His Ala Ile Gln Leu Gln Leu Glu Glu Glu Met Phe 
1               5                   10                  15      


Asn Gly Gly Ile Arg Arg Phe Glu Ala Asp Gln Gln Arg Gln Ile Ala 
            20                  25                  30          


Ala Gly Ser Glu Ser Asp Thr Ala Trp Asn Arg Arg Leu Leu Ser Glu 
        35                  40                  45              


Leu Ile Ala Pro Met Ala Glu Gly Ile Gln Ala Tyr Lys Glu Glu Tyr 
    50                  55                  60                  


Glu Gly Lys Lys Gly Arg Ala Pro Arg Ala Leu Ala Phe Leu Gln Cys 
65                  70                  75                  80  


Val Glu Asn Glu Val Ala Ala Tyr Ile Thr Met Lys Val Val Met Asp 
                85                  90                  95      


Met Leu Asn Thr Asp Ala Thr Leu Gln Ala Ile Ala Met Ser Val Ala 
            100                 105                 110         


Glu Arg Ile Glu Asp Gln Val Arg Phe Ser Lys Leu Glu Gly His Ala 
        115                 120                 125             


Ala Lys Tyr Phe Glu Lys Val Lys Lys Ser Leu Lys Ala Ser Arg Thr 
    130                 135                 140                 


Lys Ser Tyr Arg His Ala His Asn Val Ala Val Val Ala Glu Lys Ser 
145                 150                 155                 160 


Val Ala Glu Lys Asp Ala Asp Phe Asp Arg Trp Glu Ala Trp Pro Lys 
                165                 170                 175     


Glu Thr Gln Leu Gln Ile Gly Thr Thr Leu Leu Glu Ile Leu Glu Gly 
            180                 185                 190         


Ser Val Phe Tyr Asn Gly Glu Pro Val Phe Met Arg Ala Met Arg Thr 
        195                 200                 205             


Tyr Gly Gly Lys Thr Ile Tyr Tyr Leu Gln Thr Ser Glu Ser Val Gly 
    210                 215                 220                 


Gln Trp Ile Ser Ala Phe Lys Glu His Val Ala Gln Leu Ser Pro Ala 
225                 230                 235                 240 


Tyr Ala Pro Cys Val Ile Pro Pro Arg Pro Trp Arg Thr Pro Phe Asn 
                245                 250                 255     


Gly Gly Phe His Thr Glu Lys Val Ala Ser Arg Ile Arg Leu Val Lys 
            260                 265                 270         


Gly Asn Arg Glu His Val Arg Lys Leu Thr Gln Lys Gln Met Pro Lys 
        275                 280                 285             


Val Tyr Lys Ala Ile Asn Ala Leu Gln Asn Thr Gln Trp Gln Ile Asn 
    290                 295                 300                 


Lys Asp Val Leu Ala Val Ile Glu Glu Val Ile Arg Leu Asp Leu Gly 
305                 310                 315                 320 


Tyr Gly Val Pro Ser Phe Lys Pro Leu Ile Asp Lys Glu Asn Lys Pro 
                325                 330                 335     


Ala Asn Pro Val Pro Val Glu Phe Gln His Leu Arg Gly Arg Glu Leu 
            340                 345                 350         


Lys Glu Met Leu Ser Pro Glu Gln Trp Gln Gln Phe Ile Asn Trp Lys 
        355                 360                 365             


Gly Glu Cys Ala Arg Leu Tyr Thr Ala Glu Thr Lys Arg Gly Ser Lys 
    370                 375                 380                 


Ser Ala Ala Val Val Arg Met Val Gly Gln Ala Arg Lys Tyr Ser Ala 
385                 390                 395                 400 


Phe Glu Ser Ile Tyr Phe Val Tyr Ala Met Asp Ser Arg Ser Arg Val 
                405                 410                 415     


Tyr Val Gln Ser Ser Thr Leu Ser Pro Gln Ser Asn Asp Leu Gly Lys 
            420                 425                 430         


Ala Leu Leu Arg Phe Thr Glu Gly Arg Pro Val Asn Gly Val Glu Ala 
        435                 440                 445             


Leu Lys Trp Phe Cys Ile Asn Gly Ala Asn Leu Trp Gly Trp Asp Lys 
    450                 455                 460                 


Lys Thr Phe Asp Val Arg Val Ser Asn Val Leu Asp Glu Glu Phe Gln 
465                 470                 475                 480 


Asp Met Cys Arg Asp Ile Ala Ala Asp Pro Leu Thr Phe Thr Gln Trp 
                485                 490                 495     


Ala Lys Ala Asp Ala Pro Tyr Glu Phe Leu Ala Trp Cys Phe Glu Tyr 
            500                 505                 510         


Ala Gln Tyr Leu Asp Leu Val Asp Glu Gly Arg Ala Asp Glu Phe Arg 
        515                 520                 525             


Thr His Leu Pro Val His Gln Asp Gly Ser Cys Ser Gly Ile Gln His 
    530                 535                 540                 


Tyr Ser Ala Met Leu Arg Asp Glu Val Gly Ala Lys Ala Val Asn Leu 
545                 550                 555                 560 


Lys Pro Ser Asp Ala Pro Gln Asp Ile Tyr Gly Ala Val Ala Gln Val 
                565                 570                 575     


Val Ile Lys Lys Asn Ala Leu Tyr Met Asp Ala Asp Asp Ala Thr Thr 
            580                 585                 590         


Phe Thr Ser Gly Ser Val Thr Leu Ser Gly Thr Glu Leu Arg Ala Met 
        595                 600                 605             


Ala Ser Ala Trp Asp Ser Ile Gly Ile Thr Arg Ser Leu Thr Lys Lys 
    610                 615                 620                 


Pro Val Met Thr Leu Pro Tyr Gly Ser Thr Arg Leu Thr Cys Arg Glu 
625                 630                 635                 640 


Ser Val Ile Asp Tyr Ile Val Asp Leu Glu Glu Lys Glu Ala Gln Lys 
                645                 650                 655     


Ala Val Ala Glu Gly Arg Thr Ala Asn Lys Val His Pro Phe Glu Asp 
            660                 665                 670         


Asp Arg Gln Asp Tyr Leu Thr Pro Gly Ala Ala Tyr Asn Tyr Met Thr 
        675                 680                 685             


Ala Leu Ile Trp Pro Ser Ile Ser Glu Val Val Lys Ala Pro Ile Val 
    690                 695                 700                 


Ala Met Lys Met Ile Arg Gln Leu Ala Arg Phe Ala Ala Lys Arg Asn 
705                 710                 715                 720 


Glu Gly Leu Met Tyr Thr Leu Pro Thr Gly Phe Ile Leu Glu Gln Lys 
                725                 730                 735     


Ile Met Ala Thr Glu Met Leu Arg Val Arg Thr Cys Leu Met Gly Asp 
            740                 745                 750         


Ile Lys Met Ser Leu Gln Val Glu Thr Asp Ile Val Asp Glu Ala Ala 
        755                 760                 765             


Met Met Gly Ala Ala Ala Pro Asn Phe Val His Gly His Asp Ala Ser 
    770                 775                 780                 


His Leu Ile Leu Thr Val Cys Glu Leu Val Asp Lys Gly Val Thr Ser 
785                 790                 795                 800 


Ile Ala Val Ile His Asp Ser Phe Gly Thr His Ala Asp Asn Thr Leu 
                805                 810                 815     


Thr Leu Arg Val Ala Leu Lys Gly Gln Met Val Ala Met Tyr Ile Asp 
            820                 825                 830         


Gly Asn Ala Leu Gln Lys Leu Leu Glu Glu His Glu Val Arg Trp Met 
        835                 840                 845             


Val Asp Thr Gly Ile Glu Val Pro Glu Gln Gly Glu Phe Asp Leu Asn 
    850                 855                 860                 


Glu Ile Met Asp Ser Glu Tyr Val Phe Ala 
865                 870                 


<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  15
atttaggtga cactatag                                                     18


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  16
atttagggga cactatagaa gag                                               23


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  17
atttagggga cactatagaa gg                                                22


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  18
atttagggga cactatagaa ggg                                               23


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  19
atttaggtga cactatagaa                                                   20


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  20
atttaggtga cactatagaa ga                                                22


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  21
atttaggtga cactatagaa gag                                               23


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  22
atttaggtga cactatagaa gg                                                22


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  23
atttaggtga cactatagaa ggg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is a, c, g, or t

<400>  24
atttaggtga cactatagaa gng                                               23


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  25
catacgattt aggtgacact atag                                              24


