                         SEQUENCE LISTING

<110>  Purdue Research Foundation
 
<120>  CNTC Technology as a Potential Genetic Tool to Improve Stress 
       Tolerance

<130>  68822-02

<150>  US 62/915,744
<151>  2019-10-16

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  821
<212>  PRT
<213>  Arabidopsis thaliana

<400>  1

Met Glu Met Pro Gly Arg Arg Ser Asn Tyr Thr Leu Leu Ser Gln Phe 
1               5                   10                  15      


Ser Asp Asp Gln Val Ser Val Ser Val Thr Gly Ala Pro Pro Pro His 
            20                  25                  30          


Tyr Asp Ser Leu Ser Ser Glu Asn Arg Ser Asn His Asn Ser Gly Asn 
        35                  40                  45              


Thr Gly Lys Ala Lys Ala Glu Arg Gly Gly Phe Asp Trp Asp Pro Ser 
    50                  55                  60                  


Gly Gly Gly Gly Gly Asp His Arg Leu Asn Asn Gln Pro Asn Arg Val 
65                  70                  75                  80  


Gly Asn Asn Met Tyr Ala Ser Ser Leu Gly Leu Gln Arg Gln Ser Ser 
                85                  90                  95      


Gly Ser Ser Phe Gly Glu Ser Ser Leu Ser Gly Asp Tyr Tyr Met Pro 
            100                 105                 110         


Thr Leu Ser Ala Ala Ala Asn Glu Ile Glu Ser Val Gly Phe Pro Gln 
        115                 120                 125             


Asp Asp Gly Phe Arg Leu Gly Phe Gly Gly Gly Gly Gly Asp Leu Arg 
    130                 135                 140                 


Ile Gln Met Ala Ala Asp Ser Ala Gly Gly Ser Ser Ser Gly Lys Ser 
145                 150                 155                 160 


Trp Ala Gln Gln Thr Glu Glu Ser Tyr Gln Leu Gln Leu Ala Leu Ala 
                165                 170                 175     


Leu Arg Leu Ser Ser Glu Ala Thr Cys Ala Asp Asp Pro Asn Phe Leu 
            180                 185                 190         


Asp Pro Val Pro Asp Glu Ser Ala Leu Arg Thr Ser Pro Ser Ser Ala 
        195                 200                 205             


Glu Thr Val Ser His Arg Phe Trp Val Asn Gly Cys Leu Ser Tyr Tyr 
    210                 215                 220                 


Asp Lys Val Pro Asp Gly Phe Tyr Met Met Asn Gly Leu Asp Pro Tyr 
225                 230                 235                 240 


Ile Trp Thr Leu Cys Ile Asp Leu His Glu Ser Gly Arg Ile Pro Ser 
                245                 250                 255     


Ile Glu Ser Leu Arg Ala Val Asp Ser Gly Val Asp Ser Ser Leu Glu 
            260                 265                 270         


Ala Ile Ile Val Asp Arg Arg Ser Asp Pro Ala Phe Lys Glu Leu His 
        275                 280                 285             


Asn Arg Val His Asp Ile Ser Cys Ser Cys Ile Thr Thr Lys Glu Val 
    290                 295                 300                 


Val Asp Gln Leu Ala Lys Leu Ile Cys Asn Arg Met Gly Gly Pro Val 
305                 310                 315                 320 


Ile Met Gly Glu Asp Glu Leu Val Pro Met Trp Lys Glu Cys Ile Asp 
                325                 330                 335     


Gly Leu Lys Glu Ile Phe Lys Val Val Val Pro Ile Gly Ser Leu Ser 
            340                 345                 350         


Val Gly Leu Cys Arg His Arg Ala Leu Leu Phe Lys Val Leu Ala Asp 
        355                 360                 365             


Ile Ile Asp Leu Pro Cys Arg Ile Ala Lys Gly Cys Lys Tyr Cys Asn 
    370                 375                 380                 


Arg Asp Asp Ala Ala Ser Cys Leu Val Arg Phe Gly Leu Asp Arg Glu 
385                 390                 395                 400 


Tyr Leu Val Asp Leu Val Gly Lys Pro Gly His Leu Trp Glu Pro Asp 
                405                 410                 415     


Ser Leu Leu Asn Gly Pro Ser Ser Ile Ser Ile Ser Ser Pro Leu Arg 
            420                 425                 430         


Phe Pro Arg Pro Lys Pro Val Glu Pro Ala Val Asp Phe Arg Leu Leu 
        435                 440                 445             


Ala Lys Gln Tyr Phe Ser Asp Ser Gln Ser Leu Asn Leu Val Phe Asp 
    450                 455                 460                 


Pro Ala Ser Asp Asp Met Gly Phe Ser Met Phe His Arg Gln Tyr Asp 
465                 470                 475                 480 


Asn Pro Gly Gly Glu Asn Asp Ala Leu Ala Glu Asn Gly Gly Gly Ser 
                485                 490                 495     


Leu Pro Pro Ser Ala Asn Met Pro Pro Gln Asn Met Met Arg Ala Ser 
            500                 505                 510         


Asn Gln Ile Glu Ala Ala Pro Met Asn Ala Pro Pro Ile Ser Gln Pro 
        515                 520                 525             


Val Pro Asn Arg Ala Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met 
    530                 535                 540                 


Asp Ile Pro Trp Cys Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly 
545                 550                 555                 560 


Ser Phe Gly Thr Val His Arg Ala Glu Trp His Gly Ser Asp Val Ala 
                565                 570                 575     


Val Lys Ile Leu Met Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu 
            580                 585                 590         


Phe Leu Arg Glu Val Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile 
        595                 600                 605             


Val Leu Phe Met Gly Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val 
    610                 615                 620                 


Thr Glu Tyr Leu Ser Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser 
625                 630                 635                 640 


Gly Ala Arg Glu Gln Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr 
                645                 650                 655     


Asp Val Ala Lys Gly Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile 
            660                 665                 670         


Val His Arg Asp Leu Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr 
        675                 680                 685             


Thr Val Lys Val Cys Asp Phe Gly Leu Ser Arg Leu Lys Ala Ser Thr 
    690                 695                 700                 


Phe Leu Ser Ser Lys Ser Ala Ala Gly Thr Pro Glu Trp Met Ala Pro 
705                 710                 715                 720 


Glu Val Leu Arg Asp Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser 
                725                 730                 735     


Phe Gly Val Ile Leu Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly 
            740                 745                 750         


Asn Leu Asn Pro Ala Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys 
        755                 760                 765             


Arg Leu Glu Ile Pro Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile 
    770                 775                 780                 


Glu Gly Cys Trp Thr Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr 
785                 790                 795                 800 


Ile Met Asp Leu Leu Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro 
                805                 810                 815     


Asn Arg Ser Asp Leu 
            820     


<210>  2
<211>  821
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CTR1ctr1-1-inactive full length CTR1 with D694E mutation

<400>  2

Met Glu Met Pro Gly Arg Arg Ser Asn Tyr Thr Leu Leu Ser Gln Phe 
1               5                   10                  15      


Ser Asp Asp Gln Val Ser Val Ser Val Thr Gly Ala Pro Pro Pro His 
            20                  25                  30          


Tyr Asp Ser Leu Ser Ser Glu Asn Arg Ser Asn His Asn Ser Gly Asn 
        35                  40                  45              


Thr Gly Lys Ala Lys Ala Glu Arg Gly Gly Phe Asp Trp Asp Pro Ser 
    50                  55                  60                  


Gly Gly Gly Gly Gly Asp His Arg Leu Asn Asn Gln Pro Asn Arg Val 
65                  70                  75                  80  


Gly Asn Asn Met Tyr Ala Ser Ser Leu Gly Leu Gln Arg Gln Ser Ser 
                85                  90                  95      


Gly Ser Ser Phe Gly Glu Ser Ser Leu Ser Gly Asp Tyr Tyr Met Pro 
            100                 105                 110         


Thr Leu Ser Ala Ala Ala Asn Glu Ile Glu Ser Val Gly Phe Pro Gln 
        115                 120                 125             


Asp Asp Gly Phe Arg Leu Gly Phe Gly Gly Gly Gly Gly Asp Leu Arg 
    130                 135                 140                 


Ile Gln Met Ala Ala Asp Ser Ala Gly Gly Ser Ser Ser Gly Lys Ser 
145                 150                 155                 160 


Trp Ala Gln Gln Thr Glu Glu Ser Tyr Gln Leu Gln Leu Ala Leu Ala 
                165                 170                 175     


Leu Arg Leu Ser Ser Glu Ala Thr Cys Ala Asp Asp Pro Asn Phe Leu 
            180                 185                 190         


Asp Pro Val Pro Asp Glu Ser Ala Leu Arg Thr Ser Pro Ser Ser Ala 
        195                 200                 205             


Glu Thr Val Ser His Arg Phe Trp Val Asn Gly Cys Leu Ser Tyr Tyr 
    210                 215                 220                 


Asp Lys Val Pro Asp Gly Phe Tyr Met Met Asn Gly Leu Asp Pro Tyr 
225                 230                 235                 240 


Ile Trp Thr Leu Cys Ile Asp Leu His Glu Ser Gly Arg Ile Pro Ser 
                245                 250                 255     


Ile Glu Ser Leu Arg Ala Val Asp Ser Gly Val Asp Ser Ser Leu Glu 
            260                 265                 270         


Ala Ile Ile Val Asp Arg Arg Ser Asp Pro Ala Phe Lys Glu Leu His 
        275                 280                 285             


Asn Arg Val His Asp Ile Ser Cys Ser Cys Ile Thr Thr Lys Glu Val 
    290                 295                 300                 


Val Asp Gln Leu Ala Lys Leu Ile Cys Asn Arg Met Gly Gly Pro Val 
305                 310                 315                 320 


Ile Met Gly Glu Asp Glu Leu Val Pro Met Trp Lys Glu Cys Ile Asp 
                325                 330                 335     


Gly Leu Lys Glu Ile Phe Lys Val Val Val Pro Ile Gly Ser Leu Ser 
            340                 345                 350         


Val Gly Leu Cys Arg His Arg Ala Leu Leu Phe Lys Val Leu Ala Asp 
        355                 360                 365             


Ile Ile Asp Leu Pro Cys Arg Ile Ala Lys Gly Cys Lys Tyr Cys Asn 
    370                 375                 380                 


Arg Asp Asp Ala Ala Ser Cys Leu Val Arg Phe Gly Leu Asp Arg Glu 
385                 390                 395                 400 


Tyr Leu Val Asp Leu Val Gly Lys Pro Gly His Leu Trp Glu Pro Asp 
                405                 410                 415     


Ser Leu Leu Asn Gly Pro Ser Ser Ile Ser Ile Ser Ser Pro Leu Arg 
            420                 425                 430         


Phe Pro Arg Pro Lys Pro Val Glu Pro Ala Val Asp Phe Arg Leu Leu 
        435                 440                 445             


Ala Lys Gln Tyr Phe Ser Asp Ser Gln Ser Leu Asn Leu Val Phe Asp 
    450                 455                 460                 


Pro Ala Ser Asp Asp Met Gly Phe Ser Met Phe His Arg Gln Tyr Asp 
465                 470                 475                 480 


Asn Pro Gly Gly Glu Asn Asp Ala Leu Ala Glu Asn Gly Gly Gly Ser 
                485                 490                 495     


Leu Pro Pro Ser Ala Asn Met Pro Pro Gln Asn Met Met Arg Ala Ser 
            500                 505                 510         


Asn Gln Ile Glu Ala Ala Pro Met Asn Ala Pro Pro Ile Ser Gln Pro 
        515                 520                 525             


Val Pro Asn Arg Ala Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met 
    530                 535                 540                 


Asp Ile Pro Trp Cys Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly 
545                 550                 555                 560 


Ser Phe Gly Thr Val His Arg Ala Glu Trp His Gly Ser Asp Val Ala 
                565                 570                 575     


Val Lys Ile Leu Met Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu 
            580                 585                 590         


Phe Leu Arg Glu Val Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile 
        595                 600                 605             


Val Leu Phe Met Gly Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val 
    610                 615                 620                 


Thr Glu Tyr Leu Ser Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser 
625                 630                 635                 640 


Gly Ala Arg Glu Gln Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr 
                645                 650                 655     


Asp Val Ala Lys Gly Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile 
            660                 665                 670         


Val His Arg Asp Leu Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr 
        675                 680                 685             


Thr Val Lys Val Cys Glu Phe Gly Leu Ser Arg Leu Lys Ala Ser Thr 
    690                 695                 700                 


Phe Leu Ser Ser Lys Ser Ala Ala Gly Thr Pro Glu Trp Met Ala Pro 
705                 710                 715                 720 


Glu Val Leu Arg Asp Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser 
                725                 730                 735     


Phe Gly Val Ile Leu Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly 
            740                 745                 750         


Asn Leu Asn Pro Ala Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys 
        755                 760                 765             


Arg Leu Glu Ile Pro Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile 
    770                 775                 780                 


Glu Gly Cys Trp Thr Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr 
785                 790                 795                 800 


Ile Met Asp Leu Leu Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro 
                805                 810                 815     


Asn Arg Ser Asp Leu 
            820     


<210>  3
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>   delta-NT-CTR1

<400>  3

Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met Asp Ile Pro Trp Cys 
1               5                   10                  15      


Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly Ser Phe Gly Thr Val 
            20                  25                  30          


His Arg Ala Glu Trp His Gly Ser Asp Val Ala Val Lys Ile Leu Met 
        35                  40                  45              


Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu Phe Leu Arg Glu Val 
    50                  55                  60                  


Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile Val Leu Phe Met Gly 
65                  70                  75                  80  


Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val Thr Glu Tyr Leu Ser 
                85                  90                  95      


Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser Gly Ala Arg Glu Gln 
            100                 105                 110         


Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr Asp Val Ala Lys Gly 
        115                 120                 125             


Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile Val His Arg Asp Leu 
    130                 135                 140                 


Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr Thr Val Lys Val Cys 
145                 150                 155                 160 


Asp Phe Gly Leu Ser Arg Leu Lys Ala Ser Thr Phe Leu Ser Ser Lys 
                165                 170                 175     


Ser Ala Ala Gly Thr Pro Glu Trp Met Ala Pro Glu Val Leu Arg Asp 
            180                 185                 190         


Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser Phe Gly Val Ile Leu 
        195                 200                 205             


Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly Asn Leu Asn Pro Ala 
    210                 215                 220                 


Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys Arg Leu Glu Ile Pro 
225                 230                 235                 240 


Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile Glu Gly Cys Trp Thr 
                245                 250                 255     


Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr Ile Met Asp Leu Leu 
            260                 265                 270         


Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro Asn Arg Ser Asp Leu 
        275                 280                 285             


<210>  4
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Delta-NT-CTR1ctr1-1 (inactive CTR1 without the N-terminal 
       domain): D694E mutation

<400>  4

Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met Asp Ile Pro Trp Cys 
1               5                   10                  15      


Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly Ser Phe Gly Thr Val 
            20                  25                  30          


His Arg Ala Glu Trp His Gly Ser Asp Val Ala Val Lys Ile Leu Met 
        35                  40                  45              


Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu Phe Leu Arg Glu Val 
    50                  55                  60                  


Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile Val Leu Phe Met Gly 
65                  70                  75                  80  


Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val Thr Glu Tyr Leu Ser 
                85                  90                  95      


Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser Gly Ala Arg Glu Gln 
            100                 105                 110         


Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr Asp Val Ala Lys Gly 
        115                 120                 125             


Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile Val His Arg Asp Leu 
    130                 135                 140                 


Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr Thr Val Lys Val Cys 
145                 150                 155                 160 


Glu Phe Gly Leu Ser Arg Leu Lys Ala Ser Thr Phe Leu Ser Ser Lys 
                165                 170                 175     


Ser Ala Ala Gly Thr Pro Glu Trp Met Ala Pro Glu Val Leu Arg Asp 
            180                 185                 190         


Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser Phe Gly Val Ile Leu 
        195                 200                 205             


Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly Asn Leu Asn Pro Ala 
    210                 215                 220                 


Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys Arg Leu Glu Ile Pro 
225                 230                 235                 240 


Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile Glu Gly Cys Trp Thr 
                245                 250                 255     


Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr Ile Met Asp Leu Leu 
            260                 265                 270         


Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro Asn Arg Ser Asp Leu 
        275                 280                 285             


<210>  5
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  delta-NT-CTR1ctr1-1-AAA (inactive CTR1 without the N-terminal 
       domain): T704A/S707A/S710A mutation

<400>  5

Asn Arg Glu Leu Gly Leu Asp Gly Asp Asp Met Asp Ile Pro Trp Cys 
1               5                   10                  15      


Asp Leu Asn Ile Lys Glu Lys Ile Gly Ala Gly Ser Phe Gly Thr Val 
            20                  25                  30          


His Arg Ala Glu Trp His Gly Ser Asp Val Ala Val Lys Ile Leu Met 
        35                  40                  45              


Glu Gln Asp Phe His Ala Glu Arg Val Asn Glu Phe Leu Arg Glu Val 
    50                  55                  60                  


Ala Ile Met Lys Arg Leu Arg His Pro Asn Ile Val Leu Phe Met Gly 
65                  70                  75                  80  


Ala Val Thr Gln Pro Pro Asn Leu Ser Ile Val Thr Glu Tyr Leu Ser 
                85                  90                  95      


Arg Gly Ser Leu Tyr Arg Leu Leu His Lys Ser Gly Ala Arg Glu Gln 
            100                 105                 110         


Leu Asp Glu Arg Arg Arg Leu Ser Met Ala Tyr Asp Val Ala Lys Gly 
        115                 120                 125             


Met Asn Tyr Leu His Asn Arg Asn Pro Pro Ile Val His Arg Asp Leu 
    130                 135                 140                 


Lys Ser Pro Asn Leu Leu Val Asp Lys Lys Tyr Thr Val Lys Val Cys 
145                 150                 155                 160 


Asp Phe Gly Leu Ser Arg Leu Lys Ala Ser Ala Phe Leu Ala Ser Lys 
                165                 170                 175     


Ala Ala Ala Gly Thr Pro Glu Trp Met Ala Pro Glu Val Leu Arg Asp 
            180                 185                 190         


Glu Pro Ser Asn Glu Lys Ser Asp Val Tyr Ser Phe Gly Val Ile Leu 
        195                 200                 205             


Trp Glu Leu Ala Thr Leu Gln Gln Pro Trp Gly Asn Leu Asn Pro Ala 
    210                 215                 220                 


Gln Val Val Ala Ala Val Gly Phe Lys Cys Lys Arg Leu Glu Ile Pro 
225                 230                 235                 240 


Arg Asn Leu Asn Pro Gln Val Ala Ala Ile Ile Glu Gly Cys Trp Thr 
                245                 250                 255     


Asn Glu Pro Trp Lys Arg Pro Ser Phe Ala Thr Ile Met Asp Leu Leu 
            260                 265                 270         


Arg Pro Leu Ile Lys Ser Ala Val Pro Pro Pro Asn Arg Ser Asp Leu 
        275                 280                 285             


