                         SEQUENCE LISTING

<110>  Technische Universiteit Delft
 
<120>  Detection of a target polynucleotide

<130>  P6088056PCT

<150>  NL2024019
<151>  2019-10-15

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide trigger

<400>  1
tttttttttt tacatgctcg agatcagttt tttatgcgcc tgttgcc                     47


<210>  2
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Leishmania target

<400>  2
cccaaacttt tctggtcctc cgggtagggg cgttctgcga agatggaaaa atgggtgcag       60

aaaccccgtt caaaaatcgg ccaaaaatgc caaaaatcgg ctccggggcg ggaaa           115


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F2

<400>  3
cccaaacttt tctggtcctc cg                                                22


<210>  4
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R2

<400>  4
tttcccgccc cggagc                                                       16


<210>  5
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer RPA F2

<400>  5
cccaaacttt tctggtcctc cgggtagggg c                                      31


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer RPA R2

<400>  6
tttcccgccc cggagccgat ttttggcatt                                        30


<210>  7
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCA template RCA01

<400>  7
ctactacctc acctcaccca acccgcccta cccaaaaccc aacccgccct acccaaaacc       60

caacccgccc tacccaaaag gcaacaggcg cataaaacaa ctatacaac                  109


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer RCA02

<400>  8
gaggtagtag gttgtatagt                                                   20


<210>  9
<211>  105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA for Leishmania target

<400>  9
ggcagaaacc ccguucaaaa auguuuuaga gcuagaaaua gcaaguuaaa auaaggcuag       60

uccguuauca acuugaaaaa guggcaccga gucggugcuu uuuuu                      105


<210>  10
<211>  57
<212>  DNA
<213>  Leishmania aethiopica

<400>  10
cccaaacttt tctggtcctc cgggtagggg cgttctgcga agatggaaaa atgggtg          57


<210>  11
<211>  56
<212>  DNA
<213>  Leishmania major

<400>  11
ccaaactttt ctggtcctcc gggtaggggc gttctgcgaa atcggaaaaa tgggtg           56


<210>  12
<211>  56
<212>  DNA
<213>  Leishmania chagasi

<400>  12
ccaaactttt ctggtcctcc gggtaggggc gttctgcgaa aatcgaaaaa tgggtg           56


<210>  13
<211>  56
<212>  DNA
<213>  Leishmania infantum

<400>  13
ccaaactttt ctggtcctcc gggtaggggc gttctgcgaa atcggaaaaa tgggtg           56


<210>  14
<211>  57
<212>  DNA
<213>  Leishmania donovani

<400>  14
cccaaacttt tctggtcctc cgggtagggg cgttctgcaa aatcggaaaa atgggtg          57


<210>  15
<211>  57
<212>  DNA
<213>  Leishmania donovani

<400>  15
cccaaacttt tctggtcctc cgggtagggg cgttctgcaa aatcggaaaa atgggtg          57


<210>  16
<211>  57
<212>  DNA
<213>  Leishmania infantum

<400>  16
cccaaacttt tctggtcctt cgggtagggg cgttctgcga aaaccgaaaa atgggtg          57


<210>  17
<211>  57
<212>  DNA
<213>  Leishmania donovani

<400>  17
cccaaacttt tctggtcctt cgggtagggg cgttctgcga aaaccgaaaa atgggtg          57


<210>  18
<211>  57
<212>  DNA
<213>  Leishmania aethiopica

<400>  18
cccaaacttt tctggttctt cgggtagggg cgttctgcga aaaccgaaaa atgggtg          57


<210>  19
<211>  57
<212>  DNA
<213>  Leishmania infantum

<400>  19
cccaaacttt tctggtcctc cgggtagggg cgttctgcga aaaccgaaaa atgggtg          57


<210>  20
<211>  57
<212>  DNA
<213>  Leishmania tarentolae

<400>  20
cccaaacttt ttaggtccct caggtagggg cgttctgcga aaaccgaaaa atgcatg          57


<210>  21
<211>  58
<212>  DNA
<213>  Leishmania amazonensis

<400>  21
cccaaacttt tctgccccgt gggggagggg cgttctgcga ttttgggaaa aatgggtg         58


<210>  22
<211>  58
<212>  DNA
<213>  Leishmania aethiopica

<400>  22
cagaaacccc gttcaaaaat cggccaaaaa tgccaaaaat cggctccggg gcgggaaa         58


<210>  23
<211>  56
<212>  DNA
<213>  Leishmania major

<400>  23
cagaaatccc gttcaaaaat cggccaaaaa tgccaaaaat cggctccggg gcggga           56


<210>  24
<211>  58
<212>  DNA
<213>  Leishmania chagasi

<400>  24
cagaaatccc gttcaaaaat cggccaaaaa tgccaaaaat cggctccggg gcgggaaa         58


<210>  25
<211>  58
<212>  DNA
<213>  Leishmania infantum

<400>  25
cagaaatccc gttcaaaaat cggccaaaaa tgccaaaaat cggctccggg gcgggaaa         58


<210>  26
<211>  58
<212>  DNA
<213>  Leishmania donovani

<400>  26
cagaaatccc gttcaaaaat cggccaaaaa tgccaaaaat cggctccggg gcgggaaa         58


<210>  27
<211>  58
<212>  DNA
<213>  Leishmania donovani

<400>  27
cagaaatccc gttcaaaaat cggccaaaaa tgccaaaaat cggctccggg gcggaaaa         58


<210>  28
<211>  56
<212>  DNA
<213>  Leishmania infantum

<400>  28
cagaaatccc gttcaaaaat cggccaaaaa tgccaaaaat cggctccggg gcggga           56


<210>  29
<211>  57
<212>  DNA
<213>  Leishmania donovani

<400>  29
cagaaatccc gttcaaaaaa tcccaaaaat gccaaaaatc ggctccgggg cgggaaa          57


<210>  30
<211>  58
<212>  DNA
<213>  Leishmania aethiopica

<400>  30
cagaaatccc gttcaaaaaa ttgcaaaaaa tgccaaaaat cggctccggg gcgggaaa         58


<210>  31
<211>  58
<212>  DNA
<213>  Leishmania infantum

<400>  31
cagaaatccc gttcaaaaaa tgtccaaaaa tgcctaaaat cagctccgag gcgggaaa         58


<210>  32
<211>  29
<212>  DNA
<213>  Leishmania tarentolae

<400>  32
cagaaacccc gttcaaaaat cggccaaaa                                         29


<210>  33
<211>  15
<212>  DNA
<213>  Leishmania amazonensis

<400>  33
cagaaacccc gttca                                                        15


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic leishmania target FWD primer, example 4

<400>  34
cccaaacttt tctggtcctc cg                                                22


<210>  35
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic leishmania target REV primer, example 4

<400>  35
tttcccgccc cggagc                                                       16


<210>  36
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL target FWD PCR primer, example 4

<400>  36
ggggcgttct gcgaaga                                                      17


<210>  37
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL target REV PCR primer, example 4

<400>  37
gccccggagc cgat                                                         14


<210>  38
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL target FWD RPA primer, example 4

<400>  38
cccaaacttt tctggtcctc cgggtagggg c                                      31


<210>  39
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL target REV RPA primer

<400>  39
tttcccgccc cggagccgat ttttggcatt                                        30


<210>  40
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Biotinylated VL target FWD RPA primer, example 4

<400>  40
ttttttgaat tccccaaact tttctggtcc tccgggtagg ggc                         43


<210>  41
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA FWD primer, example 4

<400>  41
taatacgact cactataggc agaaaccccg ttcaaaaatg ttttagagct agaaatagca       60

agttaaaata agg                                                          73


<210>  42
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA REV primer, example 4

<400>  42
aaaaaaagca ccgactcggt gccac                                             25


<210>  43
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide trigger, example 4

<400>  43
tttttttttt tacatgctcg agatcagttt tttatgcgcc tgttgcc                     47


<210>  44
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCA02, example 4

<400>  44
ctactacctc acctcaccca acccgcccta cccaaaaccc aacccgccct acccaaaacc       60

caacccgccc tacccaaaag gcaacaggcg cataaaacaa ctatacaac                  109


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer RCA02, example 4

<400>  45
gaggtagtag gttgtatagt                                                   20


<210>  46
<211>  116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RCA03, example 4

<400>  46
ctactacctc acctcaccca acccgcccta cccaaaaccc aacccgccct acccaaaacc       60

caacccgccc tacccaaaag gcaacaggcg cataaaacac ctcagcacta tacaac          116


<210>  47
<211>  83
<212>  DNA
<213>  Leishmania major

<400>  47
ggggcgttct gcgaaatcgg aaaaatgggt gcagaaatcc cgttcaaaaa tcggccaaaa       60

atgccaaaaa tcggctccgg ggc                                               83


<210>  48
<211>  82
<212>  DNA
<213>  Leishmania chagasi

<400>  48
gggcgttctg cgaaaatcga aaaatgggtg cagaaatccc gttcaaaaat cggccaaaaa       60

tgccaaaaat cggctccggg gc                                                82


<210>  49
<211>  60
<212>  DNA
<213>  Leishmania tarentolae

<400>  49
ggggcgttct ccgaaaaccg aaaaatgcat gcagaaaccc cgttcaaaaa tcggccaaaa       60


<210>  50
<211>  83
<212>  DNA
<213>  Unknown

<220>
<223>  Leishmania species

<400>  50
ggggcgttct gcgaagatgg aaaaatgggt gcagaaaccc cgttcaaaaa tcggccaaaa       60

atgccaaaaa tcggctccgg ggc                                               83


<210>  51
<211>  83
<212>  DNA
<213>  Leishmania donovani

<400>  51
ggggcgttct gcaaaatcgg aaaaatgggt gcagaaatcc cgttcaaaaa tcggccaaaa       60

atgccaaaaa tcggctccgg ggc                                               83


<210>  52
<211>  83
<212>  DNA
<213>  Leishmania infantum

<400>  52
ggggcgttct gcgaaaaccg aaaaatgggt gcagaaatcc cgttcaaaaa tcggccaaaa       60

atgccaaaaa tcggctccgg ggc                                               83


<210>  53
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus

<400>  53
ggggcgttct gcgaaaaccg aaaaatgggt gcagaaatcc cgttcaaaaa tcggccaaaa       60

atgccaaaaa tcggctccgg ggc                                               83


