               SEQUENCE LISTING

<110> SANGAMO THERAPEUTICS FRANCE
      ABEL Tobias
      FENARD David
      GERTNER-DARDENNE Julie

<120> NOVEL CHIMERIC ANTIGEN RECEPTOR SPECIFIC FOR INTERLEUKIN-23 RECEPTOR

<130> CV-1378/PCT

<150> EP19306341.9 
<151> 2019-10-15

<160> 86

<170> BiSSAP 1.3.6

<210> 1
<211> 121
<212> PRT
<213> Artificial Sequence


<220> 
<223> VH

<400> 1
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Phe Asn Ser Asn 
            20                  25                  30          
Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              
Ile Gly Cys Ile Tyr Val Gly Ser His Val Asn Thr Tyr Tyr Ala Asn 
    50                  55                  60                  
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
65                  70                  75                  80  
Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      
Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu Tyr Phe Lys Phe Trp Gly 
            100                 105                 110         
Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     

<210> 2
<211> 113
<212> PRT
<213> Artificial Sequence


<220> 
<223> VL

<400> 2
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn 
                85                  90                  95      
Pro Asp Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110         
Gly 
    

<210> 3
<211> 113
<212> PRT
<213> Artificial Sequence


<220> 
<223> VL

<400> 3
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn 
                85                  90                  95      
Pro Asp Ile Tyr Asn Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110         
Gly 
    

<210> 4
<211> 113
<212> PRT
<213> Artificial Sequence


<220> 
<223> VL

<400> 4
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn 
                85                  90                  95      
Pro Asp Ile Tyr Asn Val Phe Gly Thr Gly Thr Lys Val Thr Val Leu 
            100                 105                 110         
Gly 
    

<210> 5
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker

<400> 5
Gly Gly Gly Ser 
1               

<210> 6
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker

<400> 6
Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      



<210> 7
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker

<400> 7
Gly Gly Gly Gly Ser 
1               5   

<210> 8
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker

<400> 8
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  

<210> 9
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker

<400> 9
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  

<210> 10
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker

<400> 10
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      
Gly Gly Gly Ser 
            20  

<210> 11
<211> 249
<212> PRT
<213> Artificial Sequence


<220> 
<223> scFv

<400> 11
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn 
                85                  90                  95      
Pro Asp Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110         
Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
    130                 135                 140                 
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Phe Asn Ser Asn 
145                 150                 155                 160 
Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
                165                 170                 175     
Ile Gly Cys Ile Tyr Val Gly Ser His Val Asn Thr Tyr Tyr Ala Asn 
            180                 185                 190         
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
        195                 200                 205             
Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 
Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu Tyr Phe Lys Phe Trp Gly 
225                 230                 235                 240 
Gln Gly Thr Leu Val Thr Val Ser Ser 
                245                 

<210> 12
<211> 249
<212> PRT
<213> Artificial Sequence


<220> 
<223> scFv

<400> 12
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn 
                85                  90                  95      
Pro Asp Ile Tyr Asn Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110         
Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
    130                 135                 140                 
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Phe Asn Ser Asn 
145                 150                 155                 160 
Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
                165                 170                 175     
Ile Gly Cys Ile Tyr Val Gly Ser His Val Asn Thr Tyr Tyr Ala Asn 
            180                 185                 190         
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
        195                 200                 205             
Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 
Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu Tyr Phe Lys Phe Trp Gly 
225                 230                 235                 240 
Gln Gly Thr Leu Val Thr Val Ser Ser 
                245                 

<210> 13
<211> 249
<212> PRT
<213> Artificial Sequence


<220> 
<223> scFv

<400> 13
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn 
                85                  90                  95      
Pro Asp Ile Tyr Asn Val Phe Gly Thr Gly Thr Lys Val Thr Val Leu 
            100                 105                 110         
Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
    130                 135                 140                 
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Phe Asn Ser Asn 
145                 150                 155                 160 
Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
                165                 170                 175     
Ile Gly Cys Ile Tyr Val Gly Ser His Val Asn Thr Tyr Tyr Ala Asn 
            180                 185                 190         
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
        195                 200                 205             
Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 
Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu Tyr Phe Lys Phe Trp Gly 
225                 230                 235                 240 
Gln Gly Thr Leu Val Thr Val Ser Ser 
                245                 

<210> 14
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Hinge

<400> 14
Ala Gly Ser Ser Ser Ser Gly Gly Ser Thr Thr Gly Gly Ser Thr Thr 
1               5                   10                  15      



<210> 15
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Hinge

<400> 15
Gly Thr Thr Ala Ala Ser Gly Ser Ser Gly Gly Ser Ser Ser Gly Ala 
1               5                   10                  15      



<210> 16
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Hinge

<400> 16
Ser Ser Ala Thr Ala Thr Ala Gly Thr Gly Ser Ser Thr Gly Ser Thr 
1               5                   10                  15      



<210> 17
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Hinge

<400> 17
Thr Ser Gly Ser Thr Gly Thr Ala Ala Ser Ser Thr Ser Thr Ser Thr 
1               5                   10                  15      



<210> 18
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Hinge

<400> 18
ggtggcggag gttctggagg tggaggttcc                                     30


<210> 19
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> Hinge

<400> 19
Lys Ile Arg Arg Asp Ser Ser 
1               5           

<210> 20
<211> 45
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD8-derived hinge domain

<400> 20
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      
Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          
Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  

<210> 21
<211> 135
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD8-derived hinge domain

<400> 21
acaacaacac ctgctcctcg gcctcctaca ccagctccta caattgccag ccagccactg     60

tctctgaggc ccgaagcttg caggcctgct gctggcggag ccgtgcatac aagaggactg    120

gatttcgcct gcgac                                                     135


<210> 22
<211> 230
<212> PRT
<213> Artificial Sequence


<220> 
<223> IgG4-derived hinge domain

<400> 22
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 
Leu Ser Leu Gly Lys Met 
225                 230 

<210> 23
<211> 690
<212> DNA
<213> Artificial Sequence


<220> 
<223> IgG4-derived hinge domain

<400> 23
gagagcaagt acggccctcc ctgcccccct tgccctgccc ccgagttcct gggcggaccc      60

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagccg gacccccgag     120

gtgacctgtg tggtggtgga cgtgtcccag gaggaccccg aggtccagtt caactggtac     180

gtggacggcg tggaggtgca caacgccaag accaagcccc gggaggagca gttcaatagc     240

acctaccggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggaa     300

tacaagtgta aggtgtccaa caagggcctg cccagcagca tcgagaaaac catcagcaag     360

gccaagggcc agcctcggga gccccaggtg tacaccctgc cccctagcca agaggagatg     420

accaagaacc aggtgtccct gacctgcctg gtgaagggct tctaccccag cgacatcgcc     480

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc ccctgtgctg     540

gacagcgacg gcagcttctt cctgtacagc cggctgaccg tggacaagag ccggtggcag     600

gagggcaacg tctttagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     660

aagagcctga gcctgtccct gggcaagatg                                      690


<210> 24
<211> 282
<212> PRT
<213> Artificial Sequence


<220> 
<223> IgD-derived hinge domain

<400> 24
Arg Trp Pro Glu Ser Pro Lys Ala Gln Ala Ser Ser Val Pro Thr Ala 
1               5                   10                  15      
Gln Pro Gln Ala Glu Gly Ser Leu Ala Lys Ala Thr Thr Ala Pro Ala 
            20                  25                  30          
Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys 
        35                  40                  45              
Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro 
    50                  55                  60                  
Ser His Thr Gln Pro Leu Gly Val Tyr Leu Leu Thr Pro Ala Val Gln 
65                  70                  75                  80  
Asp Leu Trp Leu Arg Asp Lys Ala Thr Phe Thr Cys Phe Val Val Gly 
                85                  90                  95      
Ser Asp Leu Lys Asp Ala His Leu Thr Trp Glu Val Ala Gly Lys Val 
            100                 105                 110         
Pro Thr Gly Gly Val Glu Glu Gly Leu Leu Glu Arg His Ser Asn Gly 
        115                 120                 125             
Ser Gln Ser Gln His Ser Arg Leu Thr Leu Pro Arg Ser Leu Trp Asn 
    130                 135                 140                 
Ala Gly Thr Ser Val Thr Cys Thr Leu Asn His Pro Ser Leu Pro Pro 
145                 150                 155                 160 
Gln Arg Leu Met Ala Leu Arg Glu Pro Ala Ala Gln Ala Pro Val Lys 
                165                 170                 175     
Leu Ser Leu Asn Leu Leu Ala Ser Ser Asp Pro Pro Glu Ala Ala Ser 
            180                 185                 190         
Trp Leu Leu Cys Glu Val Ser Gly Phe Ser Pro Pro Asn Ile Leu Leu 
        195                 200                 205             
Met Trp Leu Glu Asp Gln Arg Glu Val Asn Thr Ser Gly Phe Ala Pro 
    210                 215                 220                 
Ala Arg Pro Pro Pro Gln Pro Gly Ser Thr Thr Phe Trp Ala Trp Ser 
225                 230                 235                 240 
Val Leu Arg Val Pro Ala Pro Pro Ser Pro Gln Pro Ala Thr Tyr Thr 
                245                 250                 255     
Cys Val Val Ser His Glu Asp Ser Arg Thr Leu Leu Asn Ala Ser Arg 
            260                 265                 270         
Ser Leu Glu Val Ser Tyr Val Thr Asp His 
        275                 280         

<210> 25
<211> 847
<212> DNA
<213> Artificial Sequence


<220> 
<223> IgD-derived hinge domain

<400> 25
aggtggcccg aaagtcccaa ggcccaggca tctagtgttc ctactgcaca gccccaggca      60

gaaggcagcc tagccaaagc tactactgca cctgccacta cgcgcaatac tggccgtggc     120

ggggaggaga agaaaaagga gaaagagaaa gaagaacagg aagagaggga gaccaagacc     180

cctgaatgtc catcccatac ccagccgctg ggcgtctatc tcttgactcc cgcagtacag     240

gacttgtggc ttagagataa ggccaccttt acatgtttcg tcgtgggctc tgacctgaag     300

gatgcccatt tgacttggga ggttgccgga aaggtaccca cagggggggt tgaggaaggg     360

ttgctggagc gccattccaa tggctctcag agccagcact caagactcac ccttccgaga     420

tccctgtgga acgccgggac ctctgtcaca tgtactctaa atcatcctag cctgccccca     480

cagcgtctga tggcccttag agagccagcc gcccaggcac cagttaagct tagcctgaat     540

ctgctcgcca gtagtgatcc cccagaggcc gccagctggc tcttatgcga agtgtccggc     600

tttagcccgc ccaacatctt gctcatgtgg ctggaggacc agcgagaagt gaacaccagc     660

ggcttcgctc cagcccggcc cccaccccag ccgggttcta ccacattctg ggcctggagt     720

gtcttaaggg tcccagcacc acctagcccc cagccagcca catacacctg tgttgtgtcc     780

catgaagata gcaggaccct gctaaatgct tctaggagtc tggaggtttc ctacgtgact     840

gaccatt                                                               847


<210> 26
<211> 39
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD28-derived hinge domain

<400> 26
Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      
Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          
Phe Pro Gly Pro Ser Lys Pro 
        35                  

<210> 27
<211> 117
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD28-derived hinge domain

<400> 27
attgaagtta tgtatcctcc tccttaccta gacaatgaga agagcaatgg aaccattatc     60

catgtgaaag ggaaacacct ttgtccaagt cccctatttc ccggaccttc taagccc       117


<210> 28
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> 4-1BB-derived transmembrane domain

<400> 28
Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      
Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          

<210> 29
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4-1BB-derived transmembrane domain

<400> 29
atcatctcct tctttcttgc gctgacctcg actgcgttgc tcttcctgct gttcttcctc     60

acgctccgtt tctctgttgt t                                               81


<210> 30
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 30
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         



<210> 31
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 31
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         



<210> 32
<211> 113
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 32
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  
Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      
Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         
Arg 
    

<210> 33
<211> 113
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 33
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  
Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      
Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         
Arg 
    

<210> 34
<211> 336
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 34
agagtgaagt tcagcagatc cgccgacgct cctgcctatc agcagggcca aaaccagctg      60

tacaacgagc tgaacctggg gagaagagaa gagtacgacg tgctggacaa gcggagaggc     120

agagatcctg aaatgggcgg caagcccaga cggaagaatc ctcaagaggg cctgtataat     180

gagctgcaga aagacaagat ggccgaggcc tacagcgaga tcggaatgaa gggcgagcgc     240

agaagaggca agggacacga tggactgtac cagggcctga gcaccgccac caaggatacc     300

tatgatgccc tgcacatgca ggccctgcct ccaaga                               336


<210> 35
<211> 336
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 35
agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc      60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc     120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat     180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc     240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc     300

tacgacgccc ttcacatgca ggccctgccc cctcgc                               336


<210> 36
<211> 42
<212> PRT
<213> Artificial Sequence


<220> 
<223> 4-1BB-derived intracellular domain

<400> 36
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          

<210> 37
<211> 48
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD27-derived intracellular domain

<400> 37
Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      
Ala Glu Pro Cys Arg Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          
Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              



<210> 38
<211> 41
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD28-derived intracellular domain

<400> 38
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      

<210> 39
<211> 126
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4-1BB-derived intracellular domain

<400> 39
aagcggggca gaaagaaact gctctacatc ttcaagcagc ccttcatgcg gcccgtgcag     60

accacacaag aggaagatgg ctgctcctgc agattccccg aggaagaaga aggcggctgc    120

gagctg                                                               126


<210> 40
<211> 144
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD27-derived intracellular domain

<400> 40
caacgaagga aatatagatc aaacaaagga gaaagtcctg tggagcctgc agagccttgt     60

cgttacagct gccccaggga ggaggagggc agcaccatcc ccatccagga ggattaccga    120

aaaccggagc ctgcctgctc cccc                                           144


<210> 41
<211> 123
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD28-derived intracellular domain

<400> 41
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc     60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc    120

tcc                                                                  123


<210> 42
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD8-derived Leader sequence

<400> 42
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Ser 
            20          

<210> 43
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 43
Asn Trp Ser His Pro Gln Phe Glu Lys 
1               5                   

<210> 44
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tag

<400> 44
Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10  

<210> 45
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> P2A

<400> 45
Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      
Glu Glu Asn Pro Gly Pro 
            20          

<210> 46
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> GFP

<400> 46
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 47
<211> 228
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 47
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      
Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          
Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Ile Ser 
        35                  40                  45              
Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe 
    50                  55                  60                  
Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu 
65                  70                  75                  80  
Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
                85                  90                  95      
Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
            100                 105                 110         
Glu Leu Thr Arg Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
        115                 120                 125             
Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
    130                 135                 140                 
Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
145                 150                 155                 160 
Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
                165                 170                 175     
Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
            180                 185                 190         
Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
        195                 200                 205             
Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
    210                 215                 220                 
Leu Pro Pro Arg 
225             

<210> 48
<211> 684
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 48
acaacaacac ctgctcctcg gcctcctaca ccagctccta caattgccag ccagccactg      60

tctctgaggc ccgaagcttg caggcctgct gctggcggag ccgtgcatac aagaggactg     120

gatttcgcct gcgacatcat ctccttcttt cttgcgctga cctcgactgc gttgctcttc     180

ctgctgttct tcctcacgct ccgtttctct gttgttaagc ggggcagaaa gaaactgctc     240

tacatcttca agcagccctt catgcggccc gtgcagacca cacaagagga agatggctgc     300

tcctgcagat tccccgagga agaagaaggc ggctgcgagc tgacgcgtag agtgaagttc     360

agcagatccg ccgacgctcc tgcctatcag cagggccaaa accagctgta caacgagctg     420

aacctgggga gaagagaaga gtacgacgtg ctggacaagc ggagaggcag agatcctgaa     480

atgggcggca agcccagacg gaagaatcct caagagggcc tgtataatga gctgcagaaa     540

gacaagatgg ccgaggccta cagcgagatc ggaatgaagg gcgagcgcag aagaggcaag     600

ggacacgatg gactgtacca gggcctgagc accgccacca aggataccta tgatgccctg     660

cacatgcagg ccctgcctcc aaga                                            684


<210> 49
<211> 1497
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 49
atggctctgc ctgtgacagc tctgctgctg cctctggctc tgcttcttca tgccgccaga      60

ccatctgagg tgcagctagt tgaatctggt ggaggcctgg ttcagcctgg tggctctctg     120

agactgtctt gtgccgccag cggcatcgac ttcaacagca actactacat gtgctgggtc     180

cgacaggccc ccggcaaagg acttgaatgg attggctgca tctacgtggg cagccatgtg     240

aacacctact acgcaaactg ggccaagggc agattcacca tcagccggga caacagcaag     300

aacaccgtgt acctgcagat gaacagcctg agagcagaag atactgccgt gtactactgt     360

gccacatctg gcagcagcgt gctgtacttc aagttctggg gccagggcac cctggtcaca     420

gtttcttcag gaggtggagg ctctggcggt ggaggaagtg gtgggggagg ctctgacatc     480

cagatgacac agagccccag cagcctgtct gcctctgtgg gagacagagt gaccatcact     540

tgccaggcca gcgagaacat ctacagcttc ctggcctggt accagcagaa gccaggcaag     600

gcccctaagc tgctgatcta cagcgcctct aaactagctg ccggggtgcc aagcagattt     660

tctggctctg gcagcggcac cgacttcacc ctgaccatat caagcctgca gcctgaggac     720

ttcgccacct actactgcca gcagaccaac aggtacagca accccgacat ctacaacgtg     780

tttggacagg gcaccaagct gacagtgctt ggaacaacaa cacctgctcc tcggcctcct     840

acaccagctc ctacaattgc cagccagcca ctgtctctga ggcccgaagc ttgcaggcct     900

gctgctggcg gagccgtgca tacaagagga ctggatttcg cctgcgacat catctccttc     960

tttcttgcgc tgacctcgac tgcgttgctc ttcctgctgt tcttcctcac gctccgtttc    1020

tctgttgtta agcggggcag aaagaaactg ctctacatct tcaagcagcc cttcatgcgg    1080

cccgtgcaga ccacacaaga ggaagatggc tgctcctgca gattccccga ggaagaagaa    1140

ggcggctgcg agctgacgcg tagagtgaag ttcagcagat ccgccgacgc tcctgcctat    1200

cagcagggcc aaaaccagct gtacaacgag ctgaacctgg ggagaagaga agagtacgac    1260

gtgctggaca agcggagagg cagagatcct gaaatgggcg gcaagcccag acggaagaat    1320

cctcaagagg gcctgtataa tgagctgcag aaagacaaga tggccgaggc ctacagcgag    1380

atcggaatga agggcgagcg cagaagaggc aagggacacg atggactgta ccagggcctg    1440

agcaccgcca ccaaggatac ctatgatgcc ctgcacatgc aggccctgcc tccaaga       1497


<210> 50
<211> 499
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 50
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Ile Asp Phe Asn Ser Asn Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro 
    50                  55                  60                  
Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Val Gly Ser His Val 
65                  70                  75                  80  
Asn Thr Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg 
                85                  90                  95      
Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala 
            100                 105                 110         
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu 
        115                 120                 125             
Tyr Phe Lys Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
                165                 170                 175     
Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe Leu Ala 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser 
        195                 200                 205             
Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
225                 230                 235                 240 
Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn Pro Asp 
                245                 250                 255     
Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu Gly Thr 
            260                 265                 270         
Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
        275                 280                 285             
Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
    290                 295                 300                 
Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Ile Ser Phe 
305                 310                 315                 320 
Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu 
                325                 330                 335     
Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            340                 345                 350         
Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        355                 360                 365             
Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    370                 375                 380                 
Leu Thr Arg Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
385                 390                 395                 400 
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                405                 410                 415     
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            420                 425                 430         
Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        435                 440                 445             
Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    450                 455                 460                 
Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
465                 470                 475                 480 
Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                485                 490                 495     
Pro Pro Arg 
            

<210> 51
<211> 135
<212> PRT
<213> Artificial Sequence


<220> 
<223> Peptide

<400> 51
Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Glu 
1               5                   10                  15      
Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Pro 
            20                  25                  30          
Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys 
        35                  40                  45              
Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
    50                  55                  60                  
Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
65                  70                  75                  80  
Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
                85                  90                  95      
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
            100                 105                 110         
Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Val 
        115                 120                 125             
Cys Lys Cys Pro Arg Pro Trp 
    130                 135 

<210> 52
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> LAGLIDADG motif

<400> 52
Leu Ala Gly Leu Ile Asp Ala Asp Gly 
1               5                   

<210> 53
<211> 69
<212> PRT
<213> Artificial Sequence


<220> 
<223> 4-1BB-derived transmembrane and intracellular domains

<400> 53
Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      
Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys 
            20                  25                  30          
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
        35                  40                  45              
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
    50                  55                  60                  
Gly Gly Cys Glu Leu 
65                  

<210> 54
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD8-derived Leader sequence

<400> 54
atggctctgc ctgtgacagc tctgctgctg cctctggctc tgcttcttca tgccgccaga     60

ccatct                                                                66


<210> 55
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> linker

<400> 55
ggaggtggag gctctggcgg tggaggaagt ggtgggggag gctct                     45


<210> 56
<211> 363
<212> DNA
<213> Artificial Sequence


<220> 
<223> VH

<400> 56
gaggtgcagc tagttgaatc tggtggaggc ctggttcagc ctggtggctc tctgagactg      60

tcttgtgccg ccagcggcat cgacttcaac agcaactact acatgtgctg ggtccgacag     120

gcccccggca aaggacttga atggattggc tgcatctacg tgggcagcca tgtgaacacc     180

tactacgcaa actgggccaa gggcagattc accatcagcc gggacaacag caagaacacc     240

gtgtacctgc agatgaacag cctgagagca gaagatactg ccgtgtacta ctgtgccaca     300

tctggcagca gcgtgctgta cttcaagttc tggggccagg gcaccctggt cacagtttct     360

tca                                                                   363


<210> 57
<211> 339
<212> DNA
<213> Artificial Sequence


<220> 
<223> VL

<400> 57
gacatccaga tgacacagag ccccagcagc ctgtctgcct ctgtgggaga cagagtgacc      60

atcacttgcc aggccagcga gaacatctac agcttcctgg cctggtacca gcagaagcca     120

ggcaaggccc ctaagctgct gatctacagc gcctctaaac tagctgccgg ggtgccaagc     180

agattttctg gctctggcag cggcaccgac ttcaccctga ccatatcaag cctgcagcct     240

gaggacttcg ccacctacta ctgccagcag accaacaggt acagcaaccc cgacatctac     300

aacgtgtttg gacagggcac caagctgaca gtgcttgga                            339


<210> 58
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> P2A

<400> 58
ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct     60

ggacct                                                                66


<210> 59
<211> 720
<212> DNA
<213> Artificial Sequence


<220> 
<223> GFP

<400> 59
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac     120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc     180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag     240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc     300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg     360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac     420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac     480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc     540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac     600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc     660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa     720


<210> 60
<211> 67
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD8-derived hinge domain

<400> 60
Ser Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val 
1               5                   10                  15      
Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
            20                  25                  30          
Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
        35                  40                  45              
Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
    50                  55                  60                  
Ala Cys Asp 
65          

<210> 61
<211> 201
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD8-derived hinge domain

<400> 61
agcgccctga gcaacagcat catgtacttc agccacttcg tgcccgtgtt tctgcccgcc     60

aaacctacaa caacacctgc tcctcggcct cctacaccag ctcctacaat tgccagccag    120

ccactgtctc tgaggcccga agcttgcagg cctgctgctg gcggagccgt gcatacaaga    180

ggactggatt tcgcctgcga c                                              201


<210> 62
<211> 2289
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 62
atggctctgc ctgtgacagc tctgctgctg cctctggctc tgcttcttca tgccgccaga      60

ccatctgagg tgcagctagt tgaatctggt ggaggcctgg ttcagcctgg tggctctctg     120

agactgtctt gtgccgccag cggcatcgac ttcaacagca actactacat gtgctgggtc     180

cgacaggccc ccggcaaagg acttgaatgg attggctgca tctacgtggg cagccatgtg     240

aacacctact acgcaaactg ggccaagggc agattcacca tcagccggga caacagcaag     300

aacaccgtgt acctgcagat gaacagcctg agagcagaag atactgccgt gtactactgt     360

gccacatctg gcagcagcgt gctgtacttc aagttctggg gccagggcac cctggtcaca     420

gtttcttcag gaggtggagg ctctggcggt ggaggaagtg gtgggggagg ctctgacatc     480

cagatgacac agagccccag cagcctgtct gcctctgtgg gagacagagt gaccatcact     540

tgccaggcca gcgagaacat ctacagcttc ctggcctggt accagcagaa gccaggcaag     600

gcccctaagc tgctgatcta cagcgcctct aaactagctg ccggggtgcc aagcagattt     660

tctggctctg gcagcggcac cgacttcacc ctgaccatat caagcctgca gcctgaggac     720

ttcgccacct actactgcca gcagaccaac aggtacagca accccgacat ctacaacgtg     780

tttggacagg gcaccaagct gacagtgctt ggaacaacaa cacctgctcc tcggcctcct     840

acaccagctc ctacaattgc cagccagcca ctgtctctga ggcccgaagc ttgcaggcct     900

gctgctggcg gagccgtgca tacaagagga ctggatttcg cctgcgacat catctccttc     960

tttcttgcgc tgacctcgac tgcgttgctc ttcctgctgt tcttcctcac gctccgtttc    1020

tctgttgtta agcggggcag aaagaaactg ctctacatct tcaagcagcc cttcatgcgg    1080

cccgtgcaga ccacacaaga ggaagatggc tgctcctgca gattccccga ggaagaagaa    1140

ggcggctgcg agctgacgcg tagagtgaag ttcagcagat ccgccgacgc tcctgcctat    1200

cagcagggcc aaaaccagct gtacaacgag ctgaacctgg ggagaagaga agagtacgac    1260

gtgctggaca agcggagagg cagagatcct gaaatgggcg gcaagcccag acggaagaat    1320

cctcaagagg gcctgtataa tgagctgcag aaagacaaga tggccgaggc ctacagcgag    1380

atcggaatga agggcgagcg cagaagaggc aagggacacg atggactgta ccagggcctg    1440

agcaccgcca ccaaggatac ctatgatgcc ctgcacatgc aggccctgcc tccaagagct    1500

agcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac    1560

cctggaccta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc    1620

gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat    1680

gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc    1740

tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac    1800

cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc    1860

accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc    1920

gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc    1980

ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag    2040

cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg    2100

cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc    2160

gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat    2220

cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg    2280

tacaagtaa                                                            2289


<210> 63
<211> 762
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 63
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Ile Asp Phe Asn Ser Asn Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro 
    50                  55                  60                  
Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Val Gly Ser His Val 
65                  70                  75                  80  
Asn Thr Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg 
                85                  90                  95      
Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala 
            100                 105                 110         
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu 
        115                 120                 125             
Tyr Phe Lys Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
                165                 170                 175     
Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe Leu Ala 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser 
        195                 200                 205             
Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
225                 230                 235                 240 
Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn Pro Asp 
                245                 250                 255     
Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu Gly Thr 
            260                 265                 270         
Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
        275                 280                 285             
Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
    290                 295                 300                 
Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Ile Ser Phe 
305                 310                 315                 320 
Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu 
                325                 330                 335     
Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            340                 345                 350         
Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        355                 360                 365             
Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    370                 375                 380                 
Leu Thr Arg Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
385                 390                 395                 400 
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                405                 410                 415     
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            420                 425                 430         
Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        435                 440                 445             
Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    450                 455                 460                 
Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
465                 470                 475                 480 
Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                485                 490                 495     
Pro Pro Arg Ala Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys 
            500                 505                 510         
Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly 
        515                 520                 525             
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 
    530                 535                 540                 
Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 
545                 550                 555                 560 
Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 
                565                 570                 575     
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val 
            580                 585                 590         
Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe 
        595                 600                 605             
Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe 
    610                 615                 620                 
Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 
625                 630                 635                 640 
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 
                645                 650                 655     
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 
            660                 665                 670         
Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn 
        675                 680                 685             
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp 
    690                 695                 700                 
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
705                 710                 715                 720 
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn 
                725                 730                 735     
Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 
            740                 745                 750         
Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        755                 760         

<210> 64
<211> 1494
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 64
atggctctgc ctgtgacagc tctgctgctg cctctggctc tgcttcttca tgccgccaga      60

ccatctgagg tgcagctagt tgaatctggt ggaggcctgg ttcagcctgg tggctctctg     120

agactgtctt gtgccgccag cggcatcgac ttcaacagca actactacat gtgctgggtc     180

cgacaggccc ccggcaaagg acttgaatgg attggctgca tctacgtggg cagccatgtg     240

aacacctact acgcaaactg ggccaagggc agattcacca tcagccggga caacagcaag     300

aacaccgtgt acctgcagat gaacagcctg agagcagaag atactgccgt gtactactgt     360

gccacatctg gcagcagcgt gctgtacttc aagttctggg gccagggcac cctggtcaca     420

gtttcttcag gaggtggagg ctctggcggt ggaggaagtg gtgggggagg ctctgacatc     480

cagatgacac agagccccag cagcctgtct gcctctgtgg gagacagagt gaccatcact     540

tgccaggcca gcgagaacat ctacagcttc ctggcctggt accagcagaa gccaggcaag     600

gcccctaagc tgctgatcta cagcgcctct aaactagctg ccggggtgcc aagcagattt     660

tctggctctg gcagcggcac cgacttcacc ctgaccatat caagcctgca gcctgaggac     720

ttcgccacct actactgcca gcagaccaac aggtacagca accccgacat ctacaacgtg     780

tttggacagg gcaccaagct gacagtgctt ggaacaacaa cacctgctcc tcggcctcct     840

acaccagctc ctacaattgc cagccagcca ctgtctctga ggcccgaagc ttgcaggcct     900

gctgctggcg gagccgtgca tacaagagga ctggatttcg cctgcgacat catctccttc     960

tttcttgcgc tgacctcgac tgcgttgctc ttcctgctgt tcttcctcac gctccgtttc    1020

tctgttgtta agcggggcag aaagaaactg ctctacatct tcaagcagcc cttcatgcgg    1080

cccgtgcaga ccacacaaga ggaagatggc tgctcctgca gattccccga ggaagaagaa    1140

ggcggctgcg agctgagagt gaagttcagc agatccgccg acgctcctgc ctatcagcag    1200

ggccaaaacc agctctacaa cgagctgaac ctggggagaa gagaagagta cgacgtgctg    1260

gacaagcgga gaggcagaga tcctgaaatg ggcggcaagc ccagacggaa gaatcctcaa    1320

gagggcctgt ataatgagct acagaaagac aagatggcag aggcctacag cgagatcgga    1380

atgaagggcg agcgcagaag aggcaaggga cacgatggac tgtaccaggg cctgagcacc    1440

gccaccaagg atacctatga tgccctgcac atgcaggccc tgcctccaag atag          1494


<210> 65
<211> 497
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 65
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Ile Asp Phe Asn Ser Asn Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro 
    50                  55                  60                  
Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Val Gly Ser His Val 
65                  70                  75                  80  
Asn Thr Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg 
                85                  90                  95      
Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala 
            100                 105                 110         
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu 
        115                 120                 125             
Tyr Phe Lys Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
                165                 170                 175     
Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe Leu Ala 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser 
        195                 200                 205             
Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
225                 230                 235                 240 
Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn Pro Asp 
                245                 250                 255     
Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu Gly Thr 
            260                 265                 270         
Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
        275                 280                 285             
Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
    290                 295                 300                 
Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Ile Ser Phe 
305                 310                 315                 320 
Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu 
                325                 330                 335     
Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            340                 345                 350         
Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        355                 360                 365             
Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    370                 375                 380                 
Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
385                 390                 395                 400 
Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
                405                 410                 415     
Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
            420                 425                 430         
Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
        435                 440                 445             
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
    450                 455                 460                 
Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
465                 470                 475                 480 
Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
                485                 490                 495     
Arg 
    

<210> 66
<211> 226
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 66
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      
Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          
Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Ile Ser 
        35                  40                  45              
Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe 
    50                  55                  60                  
Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu 
65                  70                  75                  80  
Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
                85                  90                  95      
Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
            100                 105                 110         
Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
        115                 120                 125             
Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
    130                 135                 140                 
Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
145                 150                 155                 160 
Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
                165                 170                 175     
Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
            180                 185                 190         
Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
        195                 200                 205             
Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
    210                 215                 220                 
Pro Arg 
225     

<210> 67
<211> 681
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 67
acaacaacac ctgctcctcg gcctcctaca ccagctccta caattgccag ccagccactg      60

tctctgaggc ccgaagcttg caggcctgct gctggcggag ccgtgcatac aagaggactg     120

gatttcgcct gcgacatcat ctccttcttt cttgcgctga cctcgactgc gttgctcttc     180

ctgctgttct tcctcacgct ccgtttctct gttgttaagc ggggcagaaa gaaactgctc     240

tacatcttca agcagccctt catgcggccc gtgcagacca cacaagagga agatggctgc     300

tcctgcagat tccccgagga agaagaaggc ggctgcgagc tgagagtgaa gttcagcaga     360

tccgccgacg ctcctgccta tcagcagggc caaaaccagc tctacaacga gctgaacctg     420

gggagaagag aagagtacga cgtgctggac aagcggagag gcagagatcc tgaaatgggc     480

ggcaagccca gacggaagaa tcctcaagag ggcctgtata atgagctaca gaaagacaag     540

atggcagagg cctacagcga gatcggaatg aagggcgagc gcagaagagg caagggacac     600

gatggactgt accagggcct gagcaccgcc accaaggata cctatgatgc cctgcacatg     660

caggccctgc ctccaagata g                                               681


<210> 68
<211> 1563
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 68
atggctctgc ctgtgacagc tctgctgctg cctctggctc tgcttcttca tgccgccaga      60

ccatctgagg tgcagctagt tgaatctggt ggaggcctgg ttcagcctgg tggctctctg     120

agactgtctt gtgccgccag cggcatcgac ttcaacagca actactacat gtgctgggtc     180

cgacaggccc ccggcaaagg acttgaatgg attggctgca tctacgtggg cagccatgtg     240

aacacctact acgcaaactg ggccaagggc agattcacca tcagccggga caacagcaag     300

aacaccgtgt acctgcagat gaacagcctg agagcagaag atactgccgt gtactactgt     360

gccacatctg gcagcagcgt gctgtacttc aagttctggg gccagggcac cctggtcaca     420

gtttcttcag gaggtggagg ctctggcggt ggaggaagtg gtgggggagg ctctgacatc     480

cagatgacac agagccccag cagcctgtct gcctctgtgg gagacagagt gaccatcact     540

tgccaggcca gcgagaacat ctacagcttc ctggcctggt accagcagaa gccaggcaag     600

gcccctaagc tgctgatcta cagcgcctct aaactagctg ccggggtgcc aagcagattt     660

tctggctctg gcagcggcac cgacttcacc ctgaccatat caagcctgca gcctgaggac     720

ttcgccacct actactgcca gcagaccaac aggtacagca accccgacat ctacaacgtg     780

tttggacagg gcaccaagct gacagtgctt ggaagcgccc tgagcaacag catcatgtac     840

ttcagccact tcgtgcccgt gtttctgccc gccaaaccta caacaacacc tgctcctcgg     900

cctcctacac cagctcctac aattgccagc cagccactgt ctctgaggcc cgaagcttgc     960

aggcctgctg ctggcggagc cgtgcataca agaggactgg atttcgcctg cgacatcatc    1020

tccttctttc ttgcgctgac ctcgactgcg ttgctcttcc tgctgttctt cctcacgctc    1080

cgtttctctg ttgttaagcg gggcagaaag aaactgctct acatcttcaa gcagcccttc    1140

atgcggcccg tgcagaccac acaagaggaa gatggctgct cctgcagatt ccccgaggaa    1200

gaagaaggcg gctgcgagct gacgcgtaga gtgaagttca gcagatccgc cgacgctcct    1260

gcctatcagc agggccaaaa ccagctgtac aacgagctga acctggggag aagagaagag    1320

tacgacgtgc tggacaagcg gagaggcaga gatcctgaaa tgggcggcaa gcccagacgg    1380

aagaatcctc aagagggcct gtataatgag ctgcagaaag acaagatggc cgaggcctac    1440

agcgagatcg gaatgaaggg cgagcgcaga agaggcaagg gacacgatgg actgtaccag    1500

ggcctgagca ccgccaccaa ggatacctat gatgccctgc acatgcaggc cctgcctcca    1560

aga                                                                  1563


<210> 69
<211> 521
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 69
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Ile Asp Phe Asn Ser Asn Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro 
    50                  55                  60                  
Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Val Gly Ser His Val 
65                  70                  75                  80  
Asn Thr Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg 
                85                  90                  95      
Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala 
            100                 105                 110         
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu 
        115                 120                 125             
Tyr Phe Lys Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
                165                 170                 175     
Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe Leu Ala 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser 
        195                 200                 205             
Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
225                 230                 235                 240 
Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn Pro Asp 
                245                 250                 255     
Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu Gly Ser 
            260                 265                 270         
Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe 
        275                 280                 285             
Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
    290                 295                 300                 
Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
305                 310                 315                 320 
Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
                325                 330                 335     
Cys Asp Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu 
            340                 345                 350         
Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly 
        355                 360                 365             
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
    370                 375                 380                 
Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
385                 390                 395                 400 
Glu Glu Gly Gly Cys Glu Leu Thr Arg Arg Val Lys Phe Ser Arg Ser 
                405                 410                 415     
Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
            420                 425                 430         
Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
        435                 440                 445             
Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
    450                 455                 460                 
Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
465                 470                 475                 480 
Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                485                 490                 495     
Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            500                 505                 510         
Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520     

<210> 70
<211> 2355
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 70
atggctctgc ctgtgacagc tctgctgctg cctctggctc tgcttcttca tgccgccaga      60

ccatctgagg tgcagctagt tgaatctggt ggaggcctgg ttcagcctgg tggctctctg     120

agactgtctt gtgccgccag cggcatcgac ttcaacagca actactacat gtgctgggtc     180

cgacaggccc ccggcaaagg acttgaatgg attggctgca tctacgtggg cagccatgtg     240

aacacctact acgcaaactg ggccaagggc agattcacca tcagccggga caacagcaag     300

aacaccgtgt acctgcagat gaacagcctg agagcagaag atactgccgt gtactactgt     360

gccacatctg gcagcagcgt gctgtacttc aagttctggg gccagggcac cctggtcaca     420

gtttcttcag gaggtggagg ctctggcggt ggaggaagtg gtgggggagg ctctgacatc     480

cagatgacac agagccccag cagcctgtct gcctctgtgg gagacagagt gaccatcact     540

tgccaggcca gcgagaacat ctacagcttc ctggcctggt accagcagaa gccaggcaag     600

gcccctaagc tgctgatcta cagcgcctct aaactagctg ccggggtgcc aagcagattt     660

tctggctctg gcagcggcac cgacttcacc ctgaccatat caagcctgca gcctgaggac     720

ttcgccacct actactgcca gcagaccaac aggtacagca accccgacat ctacaacgtg     780

tttggacagg gcaccaagct gacagtgctt ggaagcgccc tgagcaacag catcatgtac     840

ttcagccact tcgtgcccgt gtttctgccc gccaaaccta caacaacacc tgctcctcgg     900

cctcctacac cagctcctac aattgccagc cagccactgt ctctgaggcc cgaagcttgc     960

aggcctgctg ctggcggagc cgtgcataca agaggactgg atttcgcctg cgacatcatc    1020

tccttctttc ttgcgctgac ctcgactgcg ttgctcttcc tgctgttctt cctcacgctc    1080

cgtttctctg ttgttaagcg gggcagaaag aaactgctct acatcttcaa gcagcccttc    1140

atgcggcccg tgcagaccac acaagaggaa gatggctgct cctgcagatt ccccgaggaa    1200

gaagaaggcg gctgcgagct gacgcgtaga gtgaagttca gcagatccgc cgacgctcct    1260

gcctatcagc agggccaaaa ccagctgtac aacgagctga acctggggag aagagaagag    1320

tacgacgtgc tggacaagcg gagaggcaga gatcctgaaa tgggcggcaa gcccagacgg    1380

aagaatcctc aagagggcct gtataatgag ctgcagaaag acaagatggc cgaggcctac    1440

agcgagatcg gaatgaaggg cgagcgcaga agaggcaagg gacacgatgg actgtaccag    1500

ggcctgagca ccgccaccaa ggatacctat gatgccctgc acatgcaggc cctgcctcca    1560

agagctagcg gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag    1620

gagaaccctg gacctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc    1680

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag    1740

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc    1800

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac    1860

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag    1920

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc    1980

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc    2040

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc    2100

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc    2160

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg    2220

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag    2280

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac    2340

gagctgtaca agtaa                                                     2355


<210> 71
<211> 784
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 71
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Ile Asp Phe Asn Ser Asn Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro 
    50                  55                  60                  
Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Val Gly Ser His Val 
65                  70                  75                  80  
Asn Thr Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg 
                85                  90                  95      
Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala 
            100                 105                 110         
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu 
        115                 120                 125             
Tyr Phe Lys Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
                165                 170                 175     
Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe Leu Ala 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser 
        195                 200                 205             
Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
225                 230                 235                 240 
Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn Pro Asp 
                245                 250                 255     
Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu Gly Ser 
            260                 265                 270         
Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe 
        275                 280                 285             
Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
    290                 295                 300                 
Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
305                 310                 315                 320 
Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
                325                 330                 335     
Cys Asp Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu 
            340                 345                 350         
Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly 
        355                 360                 365             
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
    370                 375                 380                 
Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
385                 390                 395                 400 
Glu Glu Gly Gly Cys Glu Leu Thr Arg Arg Val Lys Phe Ser Arg Ser 
                405                 410                 415     
Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
            420                 425                 430         
Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
        435                 440                 445             
Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
    450                 455                 460                 
Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
465                 470                 475                 480 
Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                485                 490                 495     
Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            500                 505                 510         
Leu His Met Gln Ala Leu Pro Pro Arg Ala Ser Gly Ser Gly Ala Thr 
        515                 520                 525             
Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
    530                 535                 540                 
Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
545                 550                 555                 560 
Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
                565                 570                 575     
Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 
            580                 585                 590         
Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
        595                 600                 605             
Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 
    610                 615                 620                 
Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
625                 630                 635                 640 
Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
                645                 650                 655     
Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
            660                 665                 670         
Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
        675                 680                 685             
Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys 
    690                 695                 700                 
Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
705                 710                 715                 720 
Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
                725                 730                 735     
Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala 
            740                 745                 750         
Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
        755                 760                 765             
Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    770                 775                 780                 



<210> 72
<211> 1560
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 72
atggctctgc ctgtgacagc tctgctgctg cctctggctc tgcttcttca tgccgccaga      60

ccatctgagg tgcagctagt tgaatctggt ggaggcctgg ttcagcctgg tggctctctg     120

agactgtctt gtgccgccag cggcatcgac ttcaacagca actactacat gtgctgggtc     180

cgacaggccc ccggcaaagg acttgaatgg attggctgca tctacgtggg cagccatgtg     240

aacacctact acgcaaactg ggccaagggc agattcacca tcagccggga caacagcaag     300

aacaccgtgt acctgcagat gaacagcctg agagcagaag atactgccgt gtactactgt     360

gccacatctg gcagcagcgt gctgtacttc aagttctggg gccagggcac cctggtcaca     420

gtttcttcag gaggtggagg ctctggcggt ggaggaagtg gtgggggagg ctctgacatc     480

cagatgacac agagccccag cagcctgtct gcctctgtgg gagacagagt gaccatcact     540

tgccaggcca gcgagaacat ctacagcttc ctggcctggt accagcagaa gccaggcaag     600

gcccctaagc tgctgatcta cagcgcctct aaactagctg ccggggtgcc aagcagattt     660

tctggctctg gcagcggcac cgacttcacc ctgaccatat caagcctgca gcctgaggac     720

ttcgccacct actactgcca gcagaccaac aggtacagca accccgacat ctacaacgtg     780

tttggacagg gcaccaagct gacagtgctt ggaagcgccc tgagcaacag catcatgtac     840

ttcagccact tcgtgcccgt gtttctgccc gccaaaccta caacaacacc tgctcctcgg     900

cctcctacac cagctcctac aattgccagc cagccactgt ctctgaggcc cgaagcttgc     960

aggcctgctg ctggcggagc cgtgcataca agaggactgg atttcgcctg cgacatcatc    1020

tccttctttc ttgcgctgac ctcgactgcg ttgctcttcc tgctgttctt cctcacgctc    1080

cgtttctctg ttgttaagcg gggcagaaag aaactgctct acatcttcaa gcagcccttc    1140

atgcggcccg tgcagaccac acaagaggaa gatggctgct cctgcagatt ccccgaggaa    1200

gaagaaggcg gctgcgagct gagagtgaag ttcagcagat ccgccgacgc tcctgcctat    1260

cagcagggcc aaaaccagct ctacaacgag ctgaacctgg ggagaagaga agagtacgac    1320

gtgctggaca agcggagagg cagagatcct gaaatgggcg gcaagcccag acggaagaat    1380

cctcaagagg gcctgtataa tgagctacag aaagacaaga tggcagaggc ctacagcgag    1440

atcggaatga agggcgagcg cagaagaggc aagggacacg atggactgta ccagggcctg    1500

agcaccgcca ccaaggatac ctatgatgcc ctgcacatgc aggccctgcc tccaagatag    1560


<210> 73
<211> 519
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 73
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Ile Asp Phe Asn Ser Asn Tyr Tyr Met Cys Trp Val Arg Gln Ala Pro 
    50                  55                  60                  
Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Tyr Val Gly Ser His Val 
65                  70                  75                  80  
Asn Thr Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg 
                85                  90                  95      
Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala 
            100                 105                 110         
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Ser Gly Ser Ser Val Leu 
        115                 120                 125             
Tyr Phe Lys Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
145                 150                 155                 160 
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
                165                 170                 175     
Val Thr Ile Thr Cys Gln Ala Ser Glu Asn Ile Tyr Ser Phe Leu Ala 
            180                 185                 190         
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser 
        195                 200                 205             
Ala Ser Lys Leu Ala Ala Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
225                 230                 235                 240 
Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Arg Tyr Ser Asn Pro Asp 
                245                 250                 255     
Ile Tyr Asn Val Phe Gly Gln Gly Thr Lys Leu Thr Val Leu Gly Ser 
            260                 265                 270         
Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe 
        275                 280                 285             
Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
    290                 295                 300                 
Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
305                 310                 315                 320 
Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
                325                 330                 335     
Cys Asp Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu 
            340                 345                 350         
Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly 
        355                 360                 365             
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
    370                 375                 380                 
Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
385                 390                 395                 400 
Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
                405                 410                 415     
Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            420                 425                 430         
Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        435                 440                 445             
Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
    450                 455                 460                 
Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
465                 470                 475                 480 
Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
                485                 490                 495     
Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
            500                 505                 510         
Met Gln Ala Leu Pro Pro Arg 
        515                 

<210> 74
<211> 250
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 74
Ser Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val 
1               5                   10                  15      
Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
            20                  25                  30          
Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
        35                  40                  45              
Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
    50                  55                  60                  
Ala Cys Asp Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu 
65                  70                  75                  80  
Leu Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg 
                85                  90                  95      
Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            100                 105                 110         
Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        115                 120                 125             
Glu Glu Glu Gly Gly Cys Glu Leu Thr Arg Arg Val Lys Phe Ser Arg 
    130                 135                 140                 
Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
145                 150                 155                 160 
Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                165                 170                 175     
Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            180                 185                 190         
Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        195                 200                 205             
Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    210                 215                 220                 
Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
225                 230                 235                 240 
Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                245                 250 

<210> 75
<211> 750
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 75
agcgccctga gcaacagcat catgtacttc agccacttcg tgcccgtgtt tctgcccgcc      60

aaacctacaa caacacctgc tcctcggcct cctacaccag ctcctacaat tgccagccag     120

ccactgtctc tgaggcccga agcttgcagg cctgctgctg gcggagccgt gcatacaaga     180

ggactggatt tcgcctgcga catcatctcc ttctttcttg cgctgacctc gactgcgttg     240

ctcttcctgc tgttcttcct cacgctccgt ttctctgttg ttaagcgggg cagaaagaaa     300

ctgctctaca tcttcaagca gcccttcatg cggcccgtgc agaccacaca agaggaagat     360

ggctgctcct gcagattccc cgaggaagaa gaaggcggct gcgagctgac gcgtagagtg     420

aagttcagca gatccgccga cgctcctgcc tatcagcagg gccaaaacca gctgtacaac     480

gagctgaacc tggggagaag agaagagtac gacgtgctgg acaagcggag aggcagagat     540

cctgaaatgg gcggcaagcc cagacggaag aatcctcaag agggcctgta taatgagctg     600

cagaaagaca agatggccga ggcctacagc gagatcggaa tgaagggcga gcgcagaaga     660

ggcaagggac acgatggact gtaccagggc ctgagcaccg ccaccaagga tacctatgat     720

gccctgcaca tgcaggccct gcctccaaga                                      750


<210> 76
<211> 248
<212> PRT
<213> Artificial Sequence


<220> 
<223> CAR

<400> 76
Ser Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val 
1               5                   10                  15      
Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
            20                  25                  30          
Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
        35                  40                  45              
Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
    50                  55                  60                  
Ala Cys Asp Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu 
65                  70                  75                  80  
Leu Phe Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg 
                85                  90                  95      
Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            100                 105                 110         
Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        115                 120                 125             
Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    130                 135                 140                 
Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
145                 150                 155                 160 
Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                165                 170                 175     
Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            180                 185                 190         
Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        195                 200                 205             
Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    210                 215                 220                 
Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
225                 230                 235                 240 
His Met Gln Ala Leu Pro Pro Arg 
                245             

<210> 77
<211> 747
<212> DNA
<213> Artificial Sequence


<220> 
<223> CAR

<400> 77
agcgccctga gcaacagcat catgtacttc agccacttcg tgcccgtgtt tctgcccgcc      60

aaacctacaa caacacctgc tcctcggcct cctacaccag ctcctacaat tgccagccag     120

ccactgtctc tgaggcccga agcttgcagg cctgctgctg gcggagccgt gcatacaaga     180

ggactggatt tcgcctgcga catcatctcc ttctttcttg cgctgacctc gactgcgttg     240

ctcttcctgc tgttcttcct cacgctccgt ttctctgttg ttaagcgggg cagaaagaaa     300

ctgctctaca tcttcaagca gcccttcatg cggcccgtgc agaccacaca agaggaagat     360

ggctgctcct gcagattccc cgaggaagaa gaaggcggct gcgagctgag agtgaagttc     420

agcagatccg ccgacgctcc tgcctatcag cagggccaaa accagctcta caacgagctg     480

aacctgggga gaagagaaga gtacgacgtg ctggacaagc ggagaggcag agatcctgaa     540

atgggcggca agcccagacg gaagaatcct caagagggcc tgtataatga gctacagaaa     600

gacaagatgg cagaggccta cagcgagatc ggaatgaagg gcgagcgcag aagaggcaag     660

ggacacgatg gactgtacca gggcctgagc accgccacca aggataccta tgatgccctg     720

cacatgcagg ccctgcctcc aagatag                                         747


<210> 78
<211> 336
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 78
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc      60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc     120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat     180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc     240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc     300

tacgacgccc ttcacatgca ggccctgccc cctcgc                               336


<210> 79
<211> 126
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4-1BB-derived intracellular domain

<400> 79
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa     60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt    120

gaactg                                                               126


<210> 80
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD8-derived Leader sequence

<400> 80
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro 
            20      

<210> 81
<211> 135
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD8-derived hinge domain

<400> 81
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg     60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg    120

gacttcgcct gtgat                                                     135


<210> 82
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cys2His2

<400> 82
Cys Cys His His 
1               

<210> 83
<211> 135
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD8-derived hinge domain

<400> 83
acaacaaccc ctgctcctcg gcctcctaca ccagctccta caattgccag ccagccactg     60

tctctgaggc ccgaagcttg tagacctgct gctggcggag ccgtgcatac aagaggactg    120

gatttcgcct gcgac                                                     135


<210> 84
<211> 123
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD28-derived intracellular domain

<400> 84
cggagcaaga gaagcagact gctgcacagc gactacatga acatgacccc tagacggccc     60

ggacctacca gaaagcacta ccagccttac gctcctccta gagacttcgc cgcctacaga    120

tcc                                                                  123


<210> 85
<211> 336
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 85
agagtgaagt tcagcagatc cgccgacgct cctgcctatc agcagggcca aaaccagctc      60

tacaacgagc tgaacctggg gagaagagaa gagtacgacg tgctggacaa gcggagaggc     120

agagatcctg aaatgggcgg caagcccaga cggaagaatc ctcaagaggg cctgtataat     180

gagctacaga aagacaagat ggcagaggcc tacagcgaga tcggaatgaa gggcgagcgc     240

agaagaggca agggacacga tggactgtac cagggcctga gcaccgccac caaggatacc     300

tatgatgccc tgcacatgca ggccctgcct ccaaga                               336


<210> 86
<211> 339
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD3zeta-derived intracellular domain

<400> 86
agagtgaagt tcagcagatc cgccgacgct cctgcctatc agcagggcca aaaccagctc      60

tacaacgagc tgaacctggg gagaagagaa gagtacgacg tgctggacaa gcggagaggc     120

agagatcctg aaatgggcgg caagcccaga cggaagaatc ctcaagaggg cctgtataat     180

gagctacaga aagacaagat ggcagaggcc tacagcgaga tcggaatgaa gggcgagcgc     240

agaagaggca agggacacga tggactgtac cagggcctga gcaccgccac caaggatacc     300

tatgatgccc tgcacatgca ggccctgcct ccaagatag                            339


