                         SEQUENCE LISTING

<110>  KWS SAAT SE & Co. KGaA
 
<120>  MAD7 nuclease in plants and expanding its PAM recognition 
       capability

<130>  KM 5450-02WO KWS0338

<150>  US 62/914,825
<151>  2019-10-14

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  1
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcaa gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atgaacctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgga cggctggtcc     1620

aagagcaagg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  2
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  2
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcaa gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atgaacctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctga cggctggtct     1620

aagagcaaag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  3
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MAD7 protein encoded by codon-optimzed MAD7 versions A and B


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  3

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Lys Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Asp Gly Trp Ser Lys Ser Lys Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  4
<211>  2503
<212>  DNA
<213>  Brachypodium distachyon

<400>  4
ggagaagaac tcgagaggga attgcagatc atgaggcaga tggctatttt tgtgtcacat       60

atgcgcaaaa agagaggcta tatttgtgtc cctaggttct tcgttgtatt gcagtttcca      120

tatcaatctg acttggtcgc atgagaaatt gatggttaaa taatttgaat ctctcatgta      180

gtatcaacta ttagatatta ttttcaccaa atatatttcc atcggagaag aagaggctac      240

agaggaagca gaagagaggg gtgggagaat ttttacactt ttgtacaccc acttaaacag      300

caaaatccgt atgaaaacag gcccaccaaa acaatgccac gataacaatc cgtagaaaca      360

aaagcttcat ttaacagcgg cgcaacaaag cacgcttatc catggtagtt gtagtccgta      420

tgcgatccaa agatcacgat tcacgcgtga cggacggacg acgcgtgcca caccacaact      480

aacggcatcc atggtagttg tagtccgtat gcgatccaaa gatcacgatt cacgcgtgac      540

ggacggacga cgcgcgccac accacaacta acagcgtgag ccagcgtcca aactccggat      600

ggcaacgggg acgaaacccg tcgggtagtc actgcccaaa cccgtccccg caaccttcat      660

cccaaacccg tccccgtttc cggtcgcggg tttcagtttt ctaccagacc cgtccccatc      720

gggtttttca tccccgtcgg gaaatccgaa cccgccagca tttcagcacc aagccaaagt      780

tgcagcagca acatgaataa aaaacaaccc gtttcaacac caagataaaa caaaacatta      840

taatttagac aacatttcac acgtataaca ataacatata gttctcacat ataacaacac      900

catttcacac ataaaacaac accatttggg ataaaaatat gggctatatc aggccatttt      960

tatgggccat attgagtttt cgtgggtttc acaggtaccg gatttgtaga atgctgaacc     1020

gggtttgaac cgtaaaatcc gcgggtattg aatttgaccc aatcccgtcg tcccctggtg     1080

gggtaaaaac accatcttga gtccaaacgg ccaccaacca aactccgacg gcaacaaaca     1140

aacggcgttg ctttgctcct cggtatctcc gtgaccgctc aatctcccgg ctgtttcccc     1200

ggaattgcgt ggactctctc atccacacgc aaaccgcctc tccctcctct ctcgtcctat     1260

ccgccccggt gccgtagcct cacgggactc ttcttcctcc cttgctataa aatccccgcc     1320

ccctcccgtc tcctctccac acatccaaac tctcaatcgc accgagaaaa atctcctagc     1380

gatcgaagcg aagcctctcc cgatcctctc aaggtacgcc cgtttcccgt cgatcctcct     1440

ccttccgttc gtgttctgta gccgatcgat tcgattccct tacacccgtt cgtgttctct     1500

cgtggatcga tcgattgttt gttgctagaa ggaactcgta gatctggcgt ttatgaactg     1560

tgattcgggt tagtccagat cgattcaggt cggtcgtcgt tgagcctctc ggctatgtct     1620

ggattatcgt gtagatctgc tggttcagtt gattatgttc ttctaggagt aatttcgttg     1680

ggtcagcgcg atttctgctt aatctatgct gcttattgcg cctgtaccta tctactaagc     1740

tatgtgcacc tgtaattttg ctagattatt cgttcatcct cgtagttggt ttgtcacagt     1800

aatccgtatg ggttctgacg atgttattgt tggtcatacc taggcttctc cagattttat     1860

tttgttaaaa ttggatagat ctgctactga tagttgatga tggaatttgg tgctgaatct     1920

atgctattta ttgcgcctat acctgatcta tcgggctatg tacggctgta gtttactgga     1980

ttattcgttc atcctcggta gttggttcat cgtttgggtt ctgacgataa tattgttgat     2040

tatgcgtagg cttctgcaga ttgttgttaa aattggatac atcggttact gatggttgat     2100

gatagatttg tgctgaacct atctgtttat tgctcctata cctgatctat agggctatgt     2160

atgcctgtaa tttaccagat tattcgttca tcctcgtagt tggttcatct ctataattcg     2220

tatgggttct tatgatgtta tcgttgatta tgcctagtct tatacagatt attgtgtcaa     2280

gattgaatat acctgctact gatcggtgat aatttggtta gtagtttgca atctgctagg     2340

aacacgttac cactgtaatc tgtaaacatg gtttgccaga gtagtttgtt ctactactct     2400

tgatatggtt gctgatttta gtcgcctcct tttggatcat gtattgatgt ccttgcagat     2460

ttccgtgtac ttaccccggc ttttgtgtac ttcgtgttaa cag                       2503


<210>  5
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MAD7 scaffold sequence

<400>  5
gtcaaaagac ctttttaatt tctactcttg tagat                                  35


<210>  6
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hammerhead Ribozyme

<400>  6
ctgatgagtc cgtgaggacg aaacgagtaa gctcgtc                                37


<210>  7
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDV Ribozyme at 3' end

<400>  7
ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg       60

aatgggac                                                                68


<210>  8
<211>  987
<212>  DNA
<213>  Zea mays

<400>  8
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta       60

agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta      120

tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa      180

tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga      240

gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt      300

ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg      360

gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt      420

agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata      480

taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa      540

aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga      600

tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag      660

cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg      720

ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg      780

gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc      840

caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc      900

ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa      960

tccacccgtc ggcacctccg cttcaag                                          987


<210>  9
<211>  520
<212>  DNA
<213>  Zea mays

<400>  9
gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc       60

catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt      120

tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat      180

tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga      240

cgggatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga      300

tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac      360

aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct      420

atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt      480

ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag                            520


<210>  10
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RR version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  10
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcaa gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atgaacctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg tggctggtcc     1620

aagagcaagg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgagggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  11
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RR version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  11
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcaa gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atgaacctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg tggctggtct     1620

aagagcaaag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgagggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  12
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7 RR encoded by cDNA of codon-optimized MAD7 RR 
       versions A and B


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  12

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Lys Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Lys Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Arg Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  13
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RVR version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  13
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcaa gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atgaacctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg tggctggtcc     1620

aagagcgtag agtactccaa gaacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  14
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RVR version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  14
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcaa gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atgaacctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg tggctggtct     1620

aagagcgtag agtacagcaa gaacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  15
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7 RVR encoded by cDNA of codon-optimized MAD7 RVR 
       versions A and B


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  15

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Lys Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Val Glu 
    530                 535                 540                 


Tyr Ser Arg Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  16
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V1 version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  16
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atgaacctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg cggctggtcc     1620

aagagcaagg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  17
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V1 version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  17
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atgaacctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg cggctggtct     1620

aagagcaaag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  18
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7-V1 encoded by cDNA of codon-optimized MAD7-V1 
       versions A and B


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  18

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Lys Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  19
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V2 version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  19
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atgaacctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg cggctggtcc     1620

aagagcaggg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  20
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V2 version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  20
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atgaacctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg cggctggtct     1620

aagagcagag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  21
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7-V2 encoded by cDNA of codon-optimized MAD7-V2 
       versions A and B


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  21

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Arg Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  22
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRR version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  22
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atgaacctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg tggctggtcc     1620

aagagcaagg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgagggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  23
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRR version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  23
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atgaacctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg tggctggtct     1620

aagagcaaag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgagggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  24
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7 RRR encoded by cDNA of codon-optimized MAD7 RRR 
       versions A and B


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  24

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Lys Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Arg Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  25
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRVR version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  25
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atgaacctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg tggctggtcc     1620

aagagcgtag agtactccaa gaacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  26
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRVR version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  26
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atgaacctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg tggctggtct     1620

aagagcgtag agtacagcaa gaacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  27
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7 RRVR encoded by cDNA of codon-optimized MAD7 RRVR 
       versions A and B


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  27

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Val Glu 
    530                 535                 540                 


Tyr Ser Arg Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  28
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V1 version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  28
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atggccctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg cggctggtcc     1620

aagagcaagg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  29
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V1 version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  29
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atggccctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg cggctggtct     1620

aagagcaaag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  30
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7-V1 + N272A


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  30

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Ala 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Lys Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  31
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V2 version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  31
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atggccctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg cggctggtcc     1620

aagagcaggg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  32
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized mutated MAD7-V2 version B

<400>  32
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atggccctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg cggctggtct     1620

aagagcagag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  33
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7-V2 + N272A


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  33

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Ala 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Arg Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  34
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRR version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  34
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atggccctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg tggctggtcc     1620

aagagcaagg agtactccaa caacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgagggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  35
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRR version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  35
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atggccctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg tggctggtct     1620

aagagcaaag agtacagcaa caacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgagggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  36
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7 RRR + N272A


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  36

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Ala 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Lys Glu 
    530                 535                 540                 


Tyr Ser Asn Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Arg Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  37
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRVR version A


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  37
atggctccaa agaagaagag gaaggtcaac aacggcacga acaacttcca gaatttcatt       60

ggcatttcca gcctccagaa gacgctccgc aacgccctga tccccaccga gaccacgcag      120

cagttcatcg tgaagaacgg catcatcaag gaggacgagc tgcgcggcga gaacaggcag      180

atcctcaagg acatcatgga cgactactac cgcggcttca tctccgagac gctctccagc      240

atcgacgaca tcgactggac gagcctgttc gagaagatgg agatccagct caagaacggc      300

gacaacaagg acaccctgat caaggagcag acggagtacc gcaaggccat ccacaagaag      360

ttcgcgaacg acgacaggtt caagaacatg ttctccgcca agctgatcag cgacatcctc      420

ccggagttcg tgatccacaa caacaactac tccgccagcg agaaggagga gaagacccag      480

gtcatcaagc tcttctcccg cttcgcgacg agcttcaagg actacttcag gaacagggcc      540

aactgcttct ccgcggacga catctccagc tccagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgcgctggtg taccgcagga tcgtcaagtc cctcagcaac      660

gacgacatca acaagatctc cggcgacatg aaggactccc tgaaggagat gagcctcgag      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aggagggcat ctccttctac      780

aacgacatct gcggcaaggt caacagcttc atggccctct actgccagaa gaacaaggag      840

aacaagaacc tctacaagct gcagaagctc cacaagcaga tcctgtgcat cgccgacacg      900

tcctacgagg tgccgtacaa gttcgagtcc gacgaggagg tgtaccagag cgtcaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagcgcc tcaggaagat cggcgacaac     1020

tacaacggct acaacctgga caagatctac atcgtgagca agttctacga gtccgtcagc     1080

cagaagacct accgcgactg ggagacgatc aacacggcgc tggagatcca ctacaacaac     1140

atcctccccg gcaacggcaa gtccaaggcc gacaaggtga agaaggcggt caagaacgac     1200

ctccagaaga gcatcaccga gatcaacgag ctggtgtcca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac gtacatccac gagatctccc acatcctgaa caacttcgag     1320

gcgcaggagc tcaagtacaa cccggagatc cacctggtcg agtccgagct caaggccagc     1380

gagctgaaga acgtgctcga cgtcatcatg aacgcgttcc actggtgcag cgtgttcatg     1440

accgaggagc tggtcgacaa ggacaacaac ttctacgccg agctcgagga gatctacgac     1500

gagatctacc ccgtgatctc cctgtacaac ctcgtgcgca actacgtcac ccagaagccg     1560

tacagcacga agaagatcaa gctcaacttc ggcatcccga ccctggcgcg tggctggtcc     1620

aagagcgtag agtactccaa gaacgcgatc atcctgatga gggacaacct gtactacctc     1680

ggcatcttca acgcgaagaa caagccggac aagaagatca tcgagggcaa cacgagcgag     1740

aacaagggcg actacaagaa gatgatctac aacctcctgc cgggccccaa caagatgatc     1800

ccgaaggtgt tcctctccag caagaccggc gtcgagacgt acaagccctc cgcctacatc     1860

ctggagggct acaagcagaa caagcacatc aagtccagca aggacttcga catcaccttc     1920

tgccacgacc tcatcgacta cttcaagaac tgcatcgcga tccaccccga gtggaagaac     1980

ttcggcttcg acttctccga caccagcacg tacgaggaca tctccggctt ctaccgcgag     2040

gtggagctgc agggctacaa gatcgactgg acgtacatca gcgagaagga catcgacctc     2100

ctgcaggaga agggccagct ctacctgttc cagatctaca acaaggactt ctccaagaag     2160

agcaccggca acgacaacct ccacacgatg tacctgaaga acctcttctc cgaggagaac     2220

ctgaaggaca tcgtcctcaa gctgaacggc gaggccgaga tcttcttcag gaagtccagc     2280

atcaagaacc cgatcatcca caagaagggc agcatcctcg tgaaccgcac ctacgaggcg     2340

gaggagaagg accagttcgg caacatccag atcgtcagga agaacatccc cgagaacatc     2400

taccaggagc tgtacaagta cttcaacgac aagtccgaca aggagctgag cgacgaggcc     2460

gcgaagctca agaacgtggt gggccaccac gaggcggcca ccaacatcgt gaaggactac     2520

cgctacacgt acgacaagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagacgggct tcatcaacga caggatcctg cagtacatcg cgaaggagaa ggacctccac     2640

gtcatcggca tcgaccgcgg cgagaggaac ctcatctacg tgtccgtcat cgacacctgc     2700

ggcaacatcg tggagcagaa gagcttcaac atcgtcaacg gctacgacta ccagatcaag     2760

ctgaagcagc aggagggcgc taggcagatc gccaggaagg agtggaagga gatcggcaag     2820

atcaaggaga tcaaggaggg ctacctctcc ctcgtgatcc acgagatcag caagatggtc     2880

atcaagtaca acgccatcat cgcgatggag gacctctcct acggcttcaa gaaggggcgc     2940

ttcaaggtgg agaggcaggt ctaccagaag ttcgagacga tgctgatcaa caagctgaac     3000

tacctcgtgt tcaaggacat cagcatcacc gagaacggcg gcctcctgaa gggctaccag     3060

ctgacgtaca tccccgacaa gctcaagaac gtgggccacc agtgcggctg catcttctac     3120

gtcccggccg cgtacacctc caagatcgac cccaccacgg gcttcgtgaa catcttcaag     3180

ttcaaggacc tcacggtcga cgccaagcgc gagttcatca agaagttcga ctccatccgc     3240

tacgacagcg agaagaacct gttctgcttc accttcgact acaacaactt catcacccag     3300

aacaccgtga tgtccaagtc cagctggagc gtgtacacgt acggcgtccg catcaagcgc     3360

aggttcgtca acggcaggtt ctccaacgag agcgacacca tcgacatcac gaaggacatg     3420

gagaagaccc tcgagatgac ggacatcaac tggagggacg gccacgacct gaggcaggac     3480

atcatcgact acgagatcgt gcagcacatc ttcgagatct tccgcctcac cgtccagatg     3540

aggaactccc tgagcgagct cgaggaccgc gactacgaca ggctgatctc cccggtgctc     3600

aacgagaaca acatcttcta cgactcggct aaggccggcg acgctctgcc caaggacgcg     3660

gacgccaacg gcgcttactg catcgcgctg aagggcctct acgagatcaa gcagatcacg     3720

gagaactgga aggaggacgg caagttcagc cgcgacaagc tcaagattag caacaaggat     3780

tggttcgatt tcattcagaa caagcggtac ctgaagcggc cagcggcgac taagaaggcg     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  38
<211>  3864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of codon-optimized MAD7 RRVR version B


<220>
<221>  NLS
<222>  (4)..(27)

<220>
<221>  NLS
<222>  (3814)..(3861)

<400>  38
atggccccga agaagaagag gaaggtcaac aacggcacca acaacttcca gaacttcatc       60

ggcatcagca gcctgcaaaa gaccctgagg aacgcgctga tcccgaccga gactacccag      120

cagttcatcg tgaagaacgg catcatcaaa gaggacgagc tgaggggcga gaacaggcag      180

atcctgaagg acatcatgga cgactactac aggggcttca tcagcgagac tctgagcagc      240

atcgacgaca tcgactggac cagcctgttc gagaagatgg aaatccagct caagaacggc      300

gacaacaagg acaccctgat caaagagcag accgagtacc gcaaggccat ccacaagaag      360

ttcgccaacg acgacaggtt caagaatatg ttcagcgcca agctgatctc cgacatcctg      420

ccagagttcg tgatccacaa caacaactac agcgccagcg agaaagaaga aaagacccag      480

gtcatcaagc tgttcagcag gttcgccacc agcttcaagg actacttcag gaacagggcc      540

aactgcttca gcgcggacga catcagctct agcagctgcc acaggatcgt gaacgacaac      600

gccgagatct tcttcagcaa cgccctggtg tacaggcgca tcgtcaagag cctgagcaac      660

gacgatatca acaagatcag cggcgacatg aaggacagcc tcaaagagat gagcctggaa      720

gagatctaca gctacgagaa gtacggcgag ttcatcaccc aagagggcat cagcttctac      780

aacgacatct gcggcaaggt gaactccttc atggccctgt actgccagaa gaacaaagag      840

aacaagaacc tctacaagct gcagaagctg cacaagcaga tcctctgcat tgccgacacc      900

tcttacgagg tgccgtacaa gttcgagagc gacgaagagg tgtaccagag cgtgaacggc      960

ttcctggaca acatctccag caagcacatc gtcgagaggc tgcgcaagat cggcgataac     1020

tacaacggct acaacctcga caagatctac atcgtgagca agttctacga gagcgtgagc     1080

caaaagacct accgcgactg ggagactatc aacaccgcgc tcgagatcca ttacaacaac     1140

atccttccag gcaacggcaa gagcaaggcc gacaaggtga agaaggccgt caagaacgac     1200

ctgcagaagt ccatcaccga gatcaacgag ctggtgagca actacaagct ctgcagcgac     1260

gacaacatca aggccgagac ttacatccac gagatcagcc acatcctgaa caacttcgag     1320

gcccaagagc tgaagtacaa cccagagatc cacctggtcg agtccgagct gaaggcctct     1380

gagctgaaga acgtgctgga cgtgatcatg aacgccttcc actggtgcag cgtgttcatg     1440

accgaggaac tggtggacaa ggataacaac ttctacgccg agcttgagga aatctacgac     1500

gagatctacc cggtgatcag cctgtacaac ctggtccgca actacgtgac ccagaagccg     1560

tacagcacca agaagatcaa gctgaacttc ggcatcccga cgctggctcg tggctggtct     1620

aagagcgtag agtacagcaa gaacgccatc atcctgatga gggacaacct ctactacctg     1680

ggcatcttca acgccaagaa caagccggat aagaagatta tcgagggcaa cacctccgag     1740

aacaagggcg actacaagaa gatgatctat aacctgctgc cgggtccgaa caagatgatc     1800

ccgaaggtgt tcctgtccag caagaccggc gtggaaacct acaagccgag cgcctacatc     1860

ctcgagggct acaagcagaa caagcacatt aagagcagca aggacttcga tatcaccttc     1920

tgccacgacc tgatcgatta ctttaagaac tgtatcgcca tccatccaga gtggaagaat     1980

ttcggcttcg acttcagcga caccagcacc tacgaggaca tcagcggttt ctaccgcgag     2040

gttgagctgc aaggctacaa gatcgattgg acctacatct ccgagaagga tatcgacctg     2100

ctgcaagaga agggccagct gtacctgttc cagatctaca acaaagactt cagcaagaag     2160

tccaccggca acgacaacct gcacaccatg tacctgaaga acctgttcag cgaggaaaac     2220

ctcaaggaca tcgtgctgaa gctgaacggc gaggcggaga tattcttccg caagtctagc     2280

atcaagaacc cgatcatcca caaaaagggc agcatcctgg tgaacaggac gtacgaggcc     2340

gaggaaaagg accagttcgg caacatccag atcgtgagga agaacatccc agagaacatc     2400

taccaagagc tttacaagta cttcaacgac aagtccgaca aagagctgag cgacgaggcc     2460

gccaagctta agaacgttgt gggccaccat gaggccgcga ccaacatcgt taaggactac     2520

cgctatacct acgataagta cttcctgcac atgccgatca ccatcaactt caaggccaac     2580

aagaccggtt tcatcaacga caggatcctc cagtacattg ccaaagaaaa ggatctgcac     2640

gttatcggca tcgacagggg cgagcgcaac ctgatctacg tgtccgtgat cgatacctgc     2700

ggcaacattg tcgagcaaaa gtccttcaac atcgtgaatg gctacgacta ccagattaag     2760

ctcaagcaac aagagggcgc tcgccagatc gctaggaaag agtggaaaga gatcggcaag     2820

atcaaagaaa tcaaagaggg ctacctgagc ctggtgatcc atgagatctc caagatggtt     2880

atcaagtaca acgcgatcat tgccatggaa gatctgtcct acggcttcaa gaaaggccgc     2940

ttcaaggtcg agcgccaggt ctaccaaaag ttcgaaacca tgctgatcaa caagctcaac     3000

tacctcgtgt tcaaggatat cagcatcact gagaacggcg gcctgctgaa gggctaccag     3060

ctgacctaca ttccagacaa gctgaaaaac gtcggccacc agtgcggctg catcttctac     3120

gttccagccg cctacaccag caagatcgac ccaactaccg gcttcgtgaa catcttcaag     3180

ttcaaggacc tgaccgtgga cgccaagagg gaattcatca agaagtttga cagcatcagg     3240

tacgacagcg agaagaacct tttctgcttc accttcgact acaacaactt tatcacccag     3300

aacaccgtga tgagcaagag cagctggtcc gtgtacacct acggcgtgag gatcaagcgc     3360

cgcttcgtta acggcaggtt ctccaacgag tccgacacca tcgacatcac caaggacatg     3420

gaaaagactc tcgagatgac cgacatcaac tggcgcgacg gccatgacct gaggcaggat     3480

atcatcgact acgagatcgt gcagcatatc ttcgagattt tcaggctgac cgtgcagatg     3540

aggaacagcc tgagcgaact cgaggacagg gactacgata ggctcatcag cccggtgctg     3600

aacgagaaca acattttcta cgactccgcc aaggccggcg acgctttgcc aaaagatgct     3660

gacgccaacg gcgcctactg cattgctctg aagggcctgt acgagatcaa gcagattacc     3720

gagaactgga aagaggatgg gaagttctcc agggataagc tgaagatcag caacaaggat     3780

tggttcgatt tcatccaaaa caagcgctac ctcaagaggc cagccgctac caagaaggct     3840

ggccaggcaa agaagaagaa gtga                                            3864


<210>  39
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein MAD7 RRVR + N272A


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  39

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Arg Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Ala 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Thr Lys Lys Ile Lys Leu 
        515                 520                 525             


Asn Phe Gly Ile Pro Thr Leu Ala Arg Gly Trp Ser Lys Ser Val Glu 
    530                 535                 540                 


Tyr Ser Arg Asn Ala Ile Ile Leu Met Arg Asp Asn Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Phe Asn Ala Lys Asn Lys Pro Asp Lys Lys Ile Ile Glu Gly 
                565                 570                 575     


Asn Thr Ser Glu Asn Lys Gly Asp Tyr Lys Lys Met Ile Tyr Asn Leu 
            580                 585                 590         


Leu Pro Gly Pro Asn Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  40
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MAD7-Cpf1 chimera I


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  40

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Lys Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu 
        515                 520                 525             


Asn Phe Gln Met Pro Thr Leu Ala Arg Gly Trp Asp Val Asn Lys Glu 
    530                 535                 540                 


Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu 
                565                 570                 575     


Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr 
            580                 585                 590         


Phe Pro Asp Ala Ala Lys Met Ile Pro Arg Cys Ser Thr Gln Leu Lys 
        595                 600                 605             


Ala Val Thr Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser 
    610                 615                 620                 


Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu 
625                 630                 635                 640 


Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys 
                645                 650                 655     


Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile 
            660                 665                 670         


Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile 
        675                 680                 685             


Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu 
    690                 695                 700                 


Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  41
<211>  1287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MAD7-Cpf1 chimera II


<220>
<221>  NLS
<222>  (2)..(9)

<220>
<221>  NLS
<222>  (1272)..(1287)

<400>  41

Met Ala Pro Lys Lys Lys Arg Lys Val Asn Asn Gly Thr Asn Asn Phe 
1               5                   10                  15      


Gln Asn Phe Ile Gly Ile Ser Ser Leu Gln Lys Thr Leu Arg Asn Ala 
            20                  25                  30          


Leu Ile Pro Thr Glu Thr Thr Gln Gln Phe Ile Val Lys Asn Gly Ile 
        35                  40                  45              


Ile Lys Glu Asp Glu Leu Arg Gly Glu Asn Arg Gln Ile Leu Lys Asp 
    50                  55                  60                  


Ile Met Asp Asp Tyr Tyr Arg Gly Phe Ile Ser Glu Thr Leu Ser Ser 
65                  70                  75                  80  


Ile Asp Asp Ile Asp Trp Thr Ser Leu Phe Glu Lys Met Glu Ile Gln 
                85                  90                  95      


Leu Lys Asn Gly Asp Asn Lys Asp Thr Leu Ile Lys Glu Gln Thr Glu 
            100                 105                 110         


Tyr Arg Lys Ala Ile His Lys Lys Phe Ala Asn Asp Asp Arg Phe Lys 
        115                 120                 125             


Asn Met Phe Ser Ala Lys Leu Ile Ser Asp Ile Leu Pro Glu Phe Val 
    130                 135                 140                 


Ile His Asn Asn Asn Tyr Ser Ala Ser Glu Lys Glu Glu Lys Thr Gln 
145                 150                 155                 160 


Val Ile Lys Leu Phe Ser Arg Phe Ala Thr Ser Phe Lys Asp Tyr Phe 
                165                 170                 175     


Lys Asn Arg Ala Asn Cys Phe Ser Ala Asp Asp Ile Ser Ser Ser Ser 
            180                 185                 190         


Cys His Arg Ile Val Asn Asp Asn Ala Glu Ile Phe Phe Ser Asn Ala 
        195                 200                 205             


Leu Val Tyr Arg Arg Ile Val Lys Ser Leu Ser Asn Asp Asp Ile Asn 
    210                 215                 220                 


Lys Ile Ser Gly Asp Met Lys Asp Ser Leu Lys Glu Met Ser Leu Glu 
225                 230                 235                 240 


Glu Ile Tyr Ser Tyr Glu Lys Tyr Gly Glu Phe Ile Thr Gln Glu Gly 
                245                 250                 255     


Ile Ser Phe Tyr Asn Asp Ile Cys Gly Lys Val Asn Ser Phe Met Asn 
            260                 265                 270         


Leu Tyr Cys Gln Lys Asn Lys Glu Asn Lys Asn Leu Tyr Lys Leu Gln 
        275                 280                 285             


Lys Leu His Lys Gln Ile Leu Cys Ile Ala Asp Thr Ser Tyr Glu Val 
    290                 295                 300                 


Pro Tyr Lys Phe Glu Ser Asp Glu Glu Val Tyr Gln Ser Val Asn Gly 
305                 310                 315                 320 


Phe Leu Asp Asn Ile Ser Ser Lys His Ile Val Glu Arg Leu Arg Lys 
                325                 330                 335     


Ile Gly Asp Asn Tyr Asn Gly Tyr Asn Leu Asp Lys Ile Tyr Ile Val 
            340                 345                 350         


Ser Lys Phe Tyr Glu Ser Val Ser Gln Lys Thr Tyr Arg Asp Trp Glu 
        355                 360                 365             


Thr Ile Asn Thr Ala Leu Glu Ile His Tyr Asn Asn Ile Leu Pro Gly 
    370                 375                 380                 


Asn Gly Lys Ser Lys Ala Asp Lys Val Lys Lys Ala Val Lys Asn Asp 
385                 390                 395                 400 


Leu Gln Lys Ser Ile Thr Glu Ile Asn Glu Leu Val Ser Asn Tyr Lys 
                405                 410                 415     


Leu Cys Ser Asp Asp Asn Ile Lys Ala Glu Thr Tyr Ile His Glu Ile 
            420                 425                 430         


Ser His Ile Leu Asn Asn Phe Glu Ala Gln Glu Leu Lys Tyr Asn Pro 
        435                 440                 445             


Glu Ile His Leu Val Glu Ser Glu Leu Lys Ala Ser Glu Leu Lys Asn 
    450                 455                 460                 


Val Leu Asp Val Ile Met Asn Ala Phe His Trp Cys Ser Val Phe Met 
465                 470                 475                 480 


Thr Glu Glu Leu Val Asp Lys Asp Asn Asn Phe Tyr Ala Glu Leu Glu 
                485                 490                 495     


Glu Ile Tyr Asp Glu Ile Tyr Pro Val Ile Ser Leu Tyr Asn Leu Val 
            500                 505                 510         


Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu 
        515                 520                 525             


Asn Phe Gln Met Pro Thr Leu Ala Arg Gly Trp Asp Val Asn Lys Glu 
    530                 535                 540                 


Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu 
545                 550                 555                 560 


Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu 
                565                 570                 575     


Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr 
            580                 585                 590         


Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Val Phe Leu Ser Ser Lys 
        595                 600                 605             


Thr Gly Val Glu Thr Tyr Lys Pro Ser Ala Tyr Ile Leu Glu Gly Tyr 
    610                 615                 620                 


Lys Gln Asn Lys His Ile Lys Ser Ser Lys Asp Phe Asp Ile Thr Phe 
625                 630                 635                 640 


Cys His Asp Leu Ile Asp Tyr Phe Lys Asn Cys Ile Ala Ile His Pro 
                645                 650                 655     


Glu Trp Lys Asn Phe Gly Phe Asp Phe Ser Asp Thr Ser Thr Tyr Glu 
            660                 665                 670         


Asp Ile Ser Gly Phe Tyr Arg Glu Val Glu Leu Gln Gly Tyr Lys Ile 
        675                 680                 685             


Asp Trp Thr Tyr Ile Ser Glu Lys Asp Ile Asp Leu Leu Gln Glu Lys 
    690                 695                 700                 


Gly Gln Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Lys Lys 
705                 710                 715                 720 


Ser Thr Gly Asn Asp Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe 
                725                 730                 735     


Ser Glu Glu Asn Leu Lys Asp Ile Val Leu Lys Leu Asn Gly Glu Ala 
            740                 745                 750         


Glu Ile Phe Phe Arg Lys Ser Ser Ile Lys Asn Pro Ile Ile His Lys 
        755                 760                 765             


Lys Gly Ser Ile Leu Val Asn Arg Thr Tyr Glu Ala Glu Glu Lys Asp 
    770                 775                 780                 


Gln Phe Gly Asn Ile Gln Ile Val Arg Lys Asn Ile Pro Glu Asn Ile 
785                 790                 795                 800 


Tyr Gln Glu Leu Tyr Lys Tyr Phe Asn Asp Lys Ser Asp Lys Glu Leu 
                805                 810                 815     


Ser Asp Glu Ala Ala Lys Leu Lys Asn Val Val Gly His His Glu Ala 
            820                 825                 830         


Ala Thr Asn Ile Val Lys Asp Tyr Arg Tyr Thr Tyr Asp Lys Tyr Phe 
        835                 840                 845             


Leu His Met Pro Ile Thr Ile Asn Phe Lys Ala Asn Lys Thr Gly Phe 
    850                 855                 860                 


Ile Asn Asp Arg Ile Leu Gln Tyr Ile Ala Lys Glu Lys Asp Leu His 
865                 870                 875                 880 


Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Val Ser Val 
                885                 890                 895     


Ile Asp Thr Cys Gly Asn Ile Val Glu Gln Lys Ser Phe Asn Ile Val 
            900                 905                 910         


Asn Gly Tyr Asp Tyr Gln Ile Lys Leu Lys Gln Gln Glu Gly Ala Arg 
        915                 920                 925             


Gln Ile Ala Arg Lys Glu Trp Lys Glu Ile Gly Lys Ile Lys Glu Ile 
    930                 935                 940                 


Lys Glu Gly Tyr Leu Ser Leu Val Ile His Glu Ile Ser Lys Met Val 
945                 950                 955                 960 


Ile Lys Tyr Asn Ala Ile Ile Ala Met Glu Asp Leu Ser Tyr Gly Phe 
                965                 970                 975     


Lys Lys Gly Arg Phe Lys Val Glu Arg Gln Val Tyr Gln Lys Phe Glu 
            980                 985                 990         


Thr Met Leu Ile Asn Lys Leu Asn  Tyr Leu Val Phe Lys  Asp Ile Ser 
        995                 1000                 1005             


Ile Thr  Glu Asn Gly Gly Leu  Leu Lys Gly Tyr Gln  Leu Thr Tyr 
    1010                 1015                 1020             


Ile Pro  Asp Lys Leu Lys Asn  Val Gly His Gln Cys  Gly Cys Ile 
    1025                 1030                 1035             


Phe Tyr  Val Pro Ala Ala Tyr  Thr Ser Lys Ile Asp  Pro Thr Thr 
    1040                 1045                 1050             


Gly Phe  Val Asn Ile Phe Lys  Phe Lys Asp Leu Thr  Val Asp Ala 
    1055                 1060                 1065             


Lys Arg  Glu Phe Ile Lys Lys  Phe Asp Ser Ile Arg  Tyr Asp Ser 
    1070                 1075                 1080             


Glu Lys  Asn Leu Phe Cys Phe  Thr Phe Asp Tyr Asn  Asn Phe Ile 
    1085                 1090                 1095             


Thr Gln  Asn Thr Val Met Ser  Lys Ser Ser Trp Ser  Val Tyr Thr 
    1100                 1105                 1110             


Tyr Gly  Val Arg Ile Lys Arg  Arg Phe Val Asn Gly  Arg Phe Ser 
    1115                 1120                 1125             


Asn Glu  Ser Asp Thr Ile Asp  Ile Thr Lys Asp Met  Glu Lys Thr 
    1130                 1135                 1140             


Leu Glu  Met Thr Asp Ile Asn  Trp Arg Asp Gly His  Asp Leu Arg 
    1145                 1150                 1155             


Gln Asp  Ile Ile Asp Tyr Glu  Ile Val Gln His Ile  Phe Glu Ile 
    1160                 1165                 1170             


Phe Arg  Leu Thr Val Gln Met  Arg Asn Ser Leu Ser  Glu Leu Glu 
    1175                 1180                 1185             


Asp Arg  Asp Tyr Asp Arg Leu  Ile Ser Pro Val Leu  Asn Glu Asn 
    1190                 1195                 1200             


Asn Ile  Phe Tyr Asp Ser Ala  Lys Ala Gly Asp Ala  Leu Pro Lys 
    1205                 1210                 1215             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Cys Ile Ala Leu  Lys Gly Leu 
    1220                 1225                 1230             


Tyr Glu  Ile Lys Gln Ile Thr  Glu Asn Trp Lys Glu  Asp Gly Lys 
    1235                 1240                 1245             


Phe Ser  Arg Asp Lys Leu Lys  Ile Ser Asn Lys Asp  Trp Phe Asp 
    1250                 1255                 1260             


Phe Ile  Gln Asn Lys Arg Tyr  Leu Lys Arg Pro Ala  Ala Thr Lys 
    1265                 1270                 1275             


Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1280                 1285         


<210>  42
<211>  1263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MAD7

<400>  42

Met Asn Asn Gly Thr Asn Asn Phe Gln Asn Phe Ile Gly Ile Ser Ser 
1               5                   10                  15      


Leu Gln Lys Thr Leu Arg Asn Ala Leu Ile Pro Thr Glu Thr Thr Gln 
            20                  25                  30          


Gln Phe Ile Val Lys Asn Gly Ile Ile Lys Glu Asp Glu Leu Arg Gly 
        35                  40                  45              


Glu Asn Arg Gln Ile Leu Lys Asp Ile Met Asp Asp Tyr Tyr Arg Gly 
    50                  55                  60                  


Phe Ile Ser Glu Thr Leu Ser Ser Ile Asp Asp Ile Asp Trp Thr Ser 
65                  70                  75                  80  


Leu Phe Glu Lys Met Glu Ile Gln Leu Lys Asn Gly Asp Asn Lys Asp 
                85                  90                  95      


Thr Leu Ile Lys Glu Gln Thr Glu Tyr Arg Lys Ala Ile His Lys Lys 
            100                 105                 110         


Phe Ala Asn Asp Asp Arg Phe Lys Asn Met Phe Ser Ala Lys Leu Ile 
        115                 120                 125             


Ser Asp Ile Leu Pro Glu Phe Val Ile His Asn Asn Asn Tyr Ser Ala 
    130                 135                 140                 


Ser Glu Lys Glu Glu Lys Thr Gln Val Ile Lys Leu Phe Ser Arg Phe 
145                 150                 155                 160 


Ala Thr Ser Phe Lys Asp Tyr Phe Lys Asn Arg Ala Asn Cys Phe Ser 
                165                 170                 175     


Ala Asp Asp Ile Ser Ser Ser Ser Cys His Arg Ile Val Asn Asp Asn 
            180                 185                 190         


Ala Glu Ile Phe Phe Ser Asn Ala Leu Val Tyr Arg Arg Ile Val Lys 
        195                 200                 205             


Ser Leu Ser Asn Asp Asp Ile Asn Lys Ile Ser Gly Asp Met Lys Asp 
    210                 215                 220                 


Ser Leu Lys Glu Met Ser Leu Glu Glu Ile Tyr Ser Tyr Glu Lys Tyr 
225                 230                 235                 240 


Gly Glu Phe Ile Thr Gln Glu Gly Ile Ser Phe Tyr Asn Asp Ile Cys 
                245                 250                 255     


Gly Lys Val Asn Ser Phe Met Asn Leu Tyr Cys Gln Lys Asn Lys Glu 
            260                 265                 270         


Asn Lys Asn Leu Tyr Lys Leu Gln Lys Leu His Lys Gln Ile Leu Cys 
        275                 280                 285             


Ile Ala Asp Thr Ser Tyr Glu Val Pro Tyr Lys Phe Glu Ser Asp Glu 
    290                 295                 300                 


Glu Val Tyr Gln Ser Val Asn Gly Phe Leu Asp Asn Ile Ser Ser Lys 
305                 310                 315                 320 


His Ile Val Glu Arg Leu Arg Lys Ile Gly Asp Asn Tyr Asn Gly Tyr 
                325                 330                 335     


Asn Leu Asp Lys Ile Tyr Ile Val Ser Lys Phe Tyr Glu Ser Val Ser 
            340                 345                 350         


Gln Lys Thr Tyr Arg Asp Trp Glu Thr Ile Asn Thr Ala Leu Glu Ile 
        355                 360                 365             


His Tyr Asn Asn Ile Leu Pro Gly Asn Gly Lys Ser Lys Ala Asp Lys 
    370                 375                 380                 


Val Lys Lys Ala Val Lys Asn Asp Leu Gln Lys Ser Ile Thr Glu Ile 
385                 390                 395                 400 


Asn Glu Leu Val Ser Asn Tyr Lys Leu Cys Ser Asp Asp Asn Ile Lys 
                405                 410                 415     


Ala Glu Thr Tyr Ile His Glu Ile Ser His Ile Leu Asn Asn Phe Glu 
            420                 425                 430         


Ala Gln Glu Leu Lys Tyr Asn Pro Glu Ile His Leu Val Glu Ser Glu 
        435                 440                 445             


Leu Lys Ala Ser Glu Leu Lys Asn Val Leu Asp Val Ile Met Asn Ala 
    450                 455                 460                 


Phe His Trp Cys Ser Val Phe Met Thr Glu Glu Leu Val Asp Lys Asp 
465                 470                 475                 480 


Asn Asn Phe Tyr Ala Glu Leu Glu Glu Ile Tyr Asp Glu Ile Tyr Pro 
                485                 490                 495     


Val Ile Ser Leu Tyr Asn Leu Val Arg Asn Tyr Val Thr Gln Lys Pro 
            500                 505                 510         


Tyr Ser Thr Lys Lys Ile Lys Leu Asn Phe Gly Ile Pro Thr Leu Ala 
        515                 520                 525             


Asp Gly Trp Ser Lys Ser Lys Glu Tyr Ser Asn Asn Ala Ile Ile Leu 
    530                 535                 540                 


Met Arg Asp Asn Leu Tyr Tyr Leu Gly Ile Phe Asn Ala Lys Asn Lys 
545                 550                 555                 560 


Pro Asp Lys Lys Ile Ile Glu Gly Asn Thr Ser Glu Asn Lys Gly Asp 
                565                 570                 575     


Tyr Lys Lys Met Ile Tyr Asn Leu Leu Pro Gly Pro Asn Lys Met Ile 
            580                 585                 590         


Pro Lys Val Phe Leu Ser Ser Lys Thr Gly Val Glu Thr Tyr Lys Pro 
        595                 600                 605             


Ser Ala Tyr Ile Leu Glu Gly Tyr Lys Gln Asn Lys His Ile Lys Ser 
    610                 615                 620                 


Ser Lys Asp Phe Asp Ile Thr Phe Cys His Asp Leu Ile Asp Tyr Phe 
625                 630                 635                 640 


Lys Asn Cys Ile Ala Ile His Pro Glu Trp Lys Asn Phe Gly Phe Asp 
                645                 650                 655     


Phe Ser Asp Thr Ser Thr Tyr Glu Asp Ile Ser Gly Phe Tyr Arg Glu 
            660                 665                 670         


Val Glu Leu Gln Gly Tyr Lys Ile Asp Trp Thr Tyr Ile Ser Glu Lys 
        675                 680                 685             


Asp Ile Asp Leu Leu Gln Glu Lys Gly Gln Leu Tyr Leu Phe Gln Ile 
    690                 695                 700                 


Tyr Asn Lys Asp Phe Ser Lys Lys Ser Thr Gly Asn Asp Asn Leu His 
705                 710                 715                 720 


Thr Met Tyr Leu Lys Asn Leu Phe Ser Glu Glu Asn Leu Lys Asp Ile 
                725                 730                 735     


Val Leu Lys Leu Asn Gly Glu Ala Glu Ile Phe Phe Arg Lys Ser Ser 
            740                 745                 750         


Ile Lys Asn Pro Ile Ile His Lys Lys Gly Ser Ile Leu Val Asn Arg 
        755                 760                 765             


Thr Tyr Glu Ala Glu Glu Lys Asp Gln Phe Gly Asn Ile Gln Ile Val 
    770                 775                 780                 


Arg Lys Asn Ile Pro Glu Asn Ile Tyr Gln Glu Leu Tyr Lys Tyr Phe 
785                 790                 795                 800 


Asn Asp Lys Ser Asp Lys Glu Leu Ser Asp Glu Ala Ala Lys Leu Lys 
                805                 810                 815     


Asn Val Val Gly His His Glu Ala Ala Thr Asn Ile Val Lys Asp Tyr 
            820                 825                 830         


Arg Tyr Thr Tyr Asp Lys Tyr Phe Leu His Met Pro Ile Thr Ile Asn 
        835                 840                 845             


Phe Lys Ala Asn Lys Thr Gly Phe Ile Asn Asp Arg Ile Leu Gln Tyr 
    850                 855                 860                 


Ile Ala Lys Glu Lys Asp Leu His Val Ile Gly Ile Asp Arg Gly Glu 
865                 870                 875                 880 


Arg Asn Leu Ile Tyr Val Ser Val Ile Asp Thr Cys Gly Asn Ile Val 
                885                 890                 895     


Glu Gln Lys Ser Phe Asn Ile Val Asn Gly Tyr Asp Tyr Gln Ile Lys 
            900                 905                 910         


Leu Lys Gln Gln Glu Gly Ala Arg Gln Ile Ala Arg Lys Glu Trp Lys 
        915                 920                 925             


Glu Ile Gly Lys Ile Lys Glu Ile Lys Glu Gly Tyr Leu Ser Leu Val 
    930                 935                 940                 


Ile His Glu Ile Ser Lys Met Val Ile Lys Tyr Asn Ala Ile Ile Ala 
945                 950                 955                 960 


Met Glu Asp Leu Ser Tyr Gly Phe Lys Lys Gly Arg Phe Lys Val Glu 
                965                 970                 975     


Arg Gln Val Tyr Gln Lys Phe Glu Thr Met Leu Ile Asn Lys Leu Asn 
            980                 985                 990         


Tyr Leu Val Phe Lys Asp Ile Ser  Ile Thr Glu Asn Gly  Gly Leu Leu 
        995                 1000                 1005             


Lys Gly  Tyr Gln Leu Thr Tyr  Ile Pro Asp Lys Leu  Lys Asn Val 
    1010                 1015                 1020             


Gly His  Gln Cys Gly Cys Ile  Phe Tyr Val Pro Ala  Ala Tyr Thr 
    1025                 1030                 1035             


Ser Lys  Ile Asp Pro Thr Thr  Gly Phe Val Asn Ile  Phe Lys Phe 
    1040                 1045                 1050             


Lys Asp  Leu Thr Val Asp Ala  Lys Arg Glu Phe Ile  Lys Lys Phe 
    1055                 1060                 1065             


Asp Ser  Ile Arg Tyr Asp Ser  Glu Lys Asn Leu Phe  Cys Phe Thr 
    1070                 1075                 1080             


Phe Asp  Tyr Asn Asn Phe Ile  Thr Gln Asn Thr Val  Met Ser Lys 
    1085                 1090                 1095             


Ser Ser  Trp Ser Val Tyr Thr  Tyr Gly Val Arg Ile  Lys Arg Arg 
    1100                 1105                 1110             


Phe Val  Asn Gly Arg Phe Ser  Asn Glu Ser Asp Thr  Ile Asp Ile 
    1115                 1120                 1125             


Thr Lys  Asp Met Glu Lys Thr  Leu Glu Met Thr Asp  Ile Asn Trp 
    1130                 1135                 1140             


Arg Asp  Gly His Asp Leu Arg  Gln Asp Ile Ile Asp  Tyr Glu Ile 
    1145                 1150                 1155             


Val Gln  His Ile Phe Glu Ile  Phe Arg Leu Thr Val  Gln Met Arg 
    1160                 1165                 1170             


Asn Ser  Leu Ser Glu Leu Glu  Asp Arg Asp Tyr Asp  Arg Leu Ile 
    1175                 1180                 1185             


Ser Pro  Val Leu Asn Glu Asn  Asn Ile Phe Tyr Asp  Ser Ala Lys 
    1190                 1195                 1200             


Ala Gly  Asp Ala Leu Pro Lys  Asp Ala Asp Ala Asn  Gly Ala Tyr 
    1205                 1210                 1215             


Cys Ile  Ala Leu Lys Gly Leu  Tyr Glu Ile Lys Gln  Ile Thr Glu 
    1220                 1225                 1230             


Asn Trp  Lys Glu Asp Gly Lys  Phe Ser Arg Asp Lys  Leu Lys Ile 
    1235                 1240                 1245             


Ser Asn  Lys Asp Trp Phe Asp  Phe Ile Gln Asn Lys  Arg Tyr Leu 
    1250                 1255                 1260             


<210>  43
<211>  1228
<212>  PRT
<213>  Lachnospiraceae

<400>  43

Met Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr Gln Glu Asn Ile Asp 
            20                  25                  30          


Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr Lys 
        35                  40                  45              


Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn Asp 
    50                  55                  60                  


Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser Leu 
65                  70                  75                  80  


Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu Asn 
                85                  90                  95      


Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly Asn 
            100                 105                 110         


Glu Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile Leu 
        115                 120                 125             


Pro Glu Phe Leu Asp Asp Lys Asp Glu Ile Ala Leu Val Asn Ser Phe 
    130                 135                 140                 


Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu Asn 
145                 150                 155                 160 


Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys Ile 
                165                 170                 175     


Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu Lys 
            180                 185                 190         


Val Asp Ala Ile Phe Asp Lys His Glu Val Gln Glu Ile Lys Glu Lys 
        195                 200                 205             


Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu Phe 
    210                 215                 220                 


Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala Ile 
225                 230                 235                 240 


Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu Asn 
                245                 250                 255     


Glu Tyr Ile Asn Leu Tyr Asn Gln Lys Thr Lys Gln Lys Leu Pro Lys 
            260                 265                 270         


Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp Arg Glu Ser Leu Ser 
        275                 280                 285             


Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu Val Leu Glu Val Phe 
    290                 295                 300                 


Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys Lys 
305                 310                 315                 320 


Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly Ile 
                325                 330                 335     


Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile Phe 
            340                 345                 350         


Gly Glu Trp Asn Val Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp Asp 
        355                 360                 365             


Ile His Leu Lys Lys Lys Ala Val Val Thr Glu Lys Tyr Glu Asp Asp 
    370                 375                 380                 


Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln Leu 
385                 390                 395                 400 


Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val Glu Lys Leu Lys Glu 
                405                 410                 415     


Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser Ser 
            420                 425                 430         


Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu Lys Ser Leu Lys Lys 
        435                 440                 445             


Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu Leu Asp Ser Val Lys 
    450                 455                 460                 


Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu Thr 
465                 470                 475                 480 


Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp Ile 
                485                 490                 495     


Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile Arg Asn Tyr Val Thr 
            500                 505                 510         


Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn Pro 
        515                 520                 525             


Gln Phe Met Gly Gly Trp Asp Lys Asp Lys Glu Thr Asp Tyr Arg Ala 
    530                 535                 540                 


Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp Lys 
545                 550                 555                 560 


Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys Asp Asp Val Asn Gly 
                565                 570                 575     


Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys Met 
            580                 585                 590         


Leu Pro Lys Val Phe Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn Pro 
        595                 600                 605             


Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys Gly 
    610                 615                 620                 


Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu Ile Asp Phe Phe Lys 
625                 630                 635                 640 


Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe Asn 
                645                 650                 655     


Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg Glu 
            660                 665                 670         


Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys Lys 
        675                 680                 685             


Glu Val Asp Lys Leu Val Glu Glu Gly Lys Leu Tyr Met Phe Gln Ile 
    690                 695                 700                 


Tyr Asn Lys Asp Phe Ser Asp Lys Ser His Gly Thr Pro Asn Leu His 
705                 710                 715                 720 


Thr Met Tyr Phe Lys Leu Leu Phe Asp Glu Asn Asn His Gly Gln Ile 
                725                 730                 735     


Arg Leu Ser Gly Gly Ala Glu Leu Phe Met Arg Arg Ala Ser Leu Lys 
            740                 745                 750         


Lys Glu Glu Leu Val Val His Pro Ala Asn Ser Pro Ile Ala Asn Lys 
        755                 760                 765             


Asn Pro Asp Asn Pro Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val Tyr 
    770                 775                 780                 


Lys Asp Lys Arg Phe Ser Glu Asp Gln Tyr Glu Leu His Ile Pro Ile 
785                 790                 795                 800 


Ala Ile Asn Lys Cys Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu Val 
                805                 810                 815     


Arg Val Leu Leu Lys His Asp Asp Asn Pro Tyr Val Ile Gly Ile Asp 
            820                 825                 830         


Arg Gly Glu Arg Asn Leu Leu Tyr Ile Val Val Val Asp Gly Lys Gly 
        835                 840                 845             


Asn Ile Val Glu Gln Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe Asn 
    850                 855                 860                 


Gly Ile Arg Ile Lys Thr Asp Tyr His Ser Leu Leu Asp Lys Lys Glu 
865                 870                 875                 880 


Lys Glu Arg Phe Glu Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn Ile 
                885                 890                 895     


Lys Glu Leu Lys Ala Gly Tyr Ile Ser Gln Val Val His Lys Ile Cys 
            900                 905                 910         


Glu Leu Val Glu Lys Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu Asn 
        915                 920                 925             


Ser Gly Phe Lys Asn Ser Arg Val Lys Val Glu Lys Gln Val Tyr Gln 
    930                 935                 940                 


Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Met Val Asp Lys 
945                 950                 955                 960 


Lys Ser Asn Pro Cys Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln Ile 
                965                 970                 975     


Thr Asn Lys Phe Glu Ser Phe Lys Ser Met Ser Thr Gln Asn Gly Phe 
            980                 985                 990         


Ile Phe Tyr Ile Pro Ala Trp Leu  Thr Ser Lys Ile Asp  Pro Ser Thr 
        995                 1000                 1005             


Gly Phe  Val Asn Leu Leu Lys  Thr Lys Tyr Thr Ser  Ile Ala Asp 
    1010                 1015                 1020             


Ser Lys  Lys Phe Ile Ser Ser  Phe Asp Arg Ile Met  Tyr Val Pro 
    1025                 1030                 1035             


Glu Glu  Asp Leu Phe Glu Phe  Ala Leu Asp Tyr Lys  Asn Phe Ser 
    1040                 1045                 1050             


Arg Thr  Asp Ala Asp Tyr Ile  Lys Lys Trp Lys Leu  Tyr Ser Tyr 
    1055                 1060                 1065             


Gly Asn  Arg Ile Arg Ile Phe  Arg Asn Pro Lys Lys  Asn Asn Val 
    1070                 1075                 1080             


Phe Asp  Trp Glu Glu Val Cys  Leu Thr Ser Ala Tyr  Lys Glu Leu 
    1085                 1090                 1095             


Phe Asn  Lys Tyr Gly Ile Asn  Tyr Gln Gln Gly Asp  Ile Arg Ala 
    1100                 1105                 1110             


Leu Leu  Cys Glu Gln Ser Asp  Lys Ala Phe Tyr Ser  Ser Phe Met 
    1115                 1120                 1125             


Ala Leu  Met Ser Leu Met Leu  Gln Met Arg Asn Ser  Ile Thr Gly 
    1130                 1135                 1140             


Arg Thr  Asp Val Asp Phe Leu  Ile Ser Pro Val Lys  Asn Ser Asp 
    1145                 1150                 1155             


Gly Ile  Phe Tyr Asp Ser Arg  Asn Tyr Glu Ala Gln  Glu Asn Ala 
    1160                 1165                 1170             


Ile Leu  Pro Lys Asn Ala Asp  Ala Asn Gly Ala Tyr  Asn Ile Ala 
    1175                 1180                 1185             


Arg Lys  Val Leu Trp Ala Ile  Gly Gln Phe Lys Lys  Ala Glu Asp 
    1190                 1195                 1200             


Glu Lys  Leu Asp Lys Val Lys  Ile Ala Ile Ser Asn  Lys Glu Trp 
    1205                 1210                 1215             


Leu Glu  Tyr Ala Gln Thr Ser  Val Lys His 
    1220                 1225             


<210>  44
<211>  1228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LbCpf1 RR

<400>  44

Met Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr Gln Glu Asn Ile Asp 
            20                  25                  30          


Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr Lys 
        35                  40                  45              


Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn Asp 
    50                  55                  60                  


Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser Leu 
65                  70                  75                  80  


Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu Asn 
                85                  90                  95      


Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly Asn 
            100                 105                 110         


Glu Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile Leu 
        115                 120                 125             


Pro Glu Phe Leu Asp Asp Lys Asp Glu Ile Ala Leu Val Asn Ser Phe 
    130                 135                 140                 


Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu Asn 
145                 150                 155                 160 


Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys Ile 
                165                 170                 175     


Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu Lys 
            180                 185                 190         


Val Asp Ala Ile Phe Asp Lys His Glu Val Gln Glu Ile Lys Glu Lys 
        195                 200                 205             


Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu Phe 
    210                 215                 220                 


Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala Ile 
225                 230                 235                 240 


Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu Asn 
                245                 250                 255     


Glu Tyr Ile Asn Leu Tyr Asn Gln Lys Thr Lys Gln Lys Leu Pro Lys 
            260                 265                 270         


Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp Arg Glu Ser Leu Ser 
        275                 280                 285             


Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu Val Leu Glu Val Phe 
    290                 295                 300                 


Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys Lys 
305                 310                 315                 320 


Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly Ile 
                325                 330                 335     


Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile Phe 
            340                 345                 350         


Gly Glu Trp Asn Val Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp Asp 
        355                 360                 365             


Ile His Leu Lys Lys Lys Ala Val Val Thr Glu Lys Tyr Glu Asp Asp 
    370                 375                 380                 


Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln Leu 
385                 390                 395                 400 


Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val Glu Lys Leu Lys Glu 
                405                 410                 415     


Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser Ser 
            420                 425                 430         


Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu Lys Ser Leu Lys Lys 
        435                 440                 445             


Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu Leu Asp Ser Val Lys 
    450                 455                 460                 


Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu Thr 
465                 470                 475                 480 


Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp Ile 
                485                 490                 495     


Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile Arg Asn Tyr Val Thr 
            500                 505                 510         


Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn Pro 
        515                 520                 525             


Gln Phe Met Arg Gly Trp Asp Lys Asp Lys Glu Thr Asp Tyr Arg Ala 
    530                 535                 540                 


Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp Lys 
545                 550                 555                 560 


Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys Asp Asp Val Asn Gly 
                565                 570                 575     


Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys Met 
            580                 585                 590         


Leu Pro Arg Val Phe Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn Pro 
        595                 600                 605             


Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys Gly 
    610                 615                 620                 


Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu Ile Asp Phe Phe Lys 
625                 630                 635                 640 


Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe Asn 
                645                 650                 655     


Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg Glu 
            660                 665                 670         


Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys Lys 
        675                 680                 685             


Glu Val Asp Lys Leu Val Glu Glu Gly Lys Leu Tyr Met Phe Gln Ile 
    690                 695                 700                 


Tyr Asn Lys Asp Phe Ser Asp Lys Ser His Gly Thr Pro Asn Leu His 
705                 710                 715                 720 


Thr Met Tyr Phe Lys Leu Leu Phe Asp Glu Asn Asn His Gly Gln Ile 
                725                 730                 735     


Arg Leu Ser Gly Gly Ala Glu Leu Phe Met Arg Arg Ala Ser Leu Lys 
            740                 745                 750         


Lys Glu Glu Leu Val Val His Pro Ala Asn Ser Pro Ile Ala Asn Lys 
        755                 760                 765             


Asn Pro Asp Asn Pro Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val Tyr 
    770                 775                 780                 


Lys Asp Lys Arg Phe Ser Glu Asp Gln Tyr Glu Leu His Ile Pro Ile 
785                 790                 795                 800 


Ala Ile Asn Lys Cys Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu Val 
                805                 810                 815     


Arg Val Leu Leu Lys His Asp Asp Asn Pro Tyr Val Ile Gly Ile Asp 
            820                 825                 830         


Arg Gly Glu Arg Asn Leu Leu Tyr Ile Val Val Val Asp Gly Lys Gly 
        835                 840                 845             


Asn Ile Val Glu Gln Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe Asn 
    850                 855                 860                 


Gly Ile Arg Ile Lys Thr Asp Tyr His Ser Leu Leu Asp Lys Lys Glu 
865                 870                 875                 880 


Lys Glu Arg Phe Glu Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn Ile 
                885                 890                 895     


Lys Glu Leu Lys Ala Gly Tyr Ile Ser Gln Val Val His Lys Ile Cys 
            900                 905                 910         


Glu Leu Val Glu Lys Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu Asn 
        915                 920                 925             


Ser Gly Phe Lys Asn Ser Arg Val Lys Val Glu Lys Gln Val Tyr Gln 
    930                 935                 940                 


Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Met Val Asp Lys 
945                 950                 955                 960 


Lys Ser Asn Pro Cys Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln Ile 
                965                 970                 975     


Thr Asn Lys Phe Glu Ser Phe Lys Ser Met Ser Thr Gln Asn Gly Phe 
            980                 985                 990         


Ile Phe Tyr Ile Pro Ala Trp Leu  Thr Ser Lys Ile Asp  Pro Ser Thr 
        995                 1000                 1005             


Gly Phe  Val Asn Leu Leu Lys  Thr Lys Tyr Thr Ser  Ile Ala Asp 
    1010                 1015                 1020             


Ser Lys  Lys Phe Ile Ser Ser  Phe Asp Arg Ile Met  Tyr Val Pro 
    1025                 1030                 1035             


Glu Glu  Asp Leu Phe Glu Phe  Ala Leu Asp Tyr Lys  Asn Phe Ser 
    1040                 1045                 1050             


Arg Thr  Asp Ala Asp Tyr Ile  Lys Lys Trp Lys Leu  Tyr Ser Tyr 
    1055                 1060                 1065             


Gly Asn  Arg Ile Arg Ile Phe  Arg Asn Pro Lys Lys  Asn Asn Val 
    1070                 1075                 1080             


Phe Asp  Trp Glu Glu Val Cys  Leu Thr Ser Ala Tyr  Lys Glu Leu 
    1085                 1090                 1095             


Phe Asn  Lys Tyr Gly Ile Asn  Tyr Gln Gln Gly Asp  Ile Arg Ala 
    1100                 1105                 1110             


Leu Leu  Cys Glu Gln Ser Asp  Lys Ala Phe Tyr Ser  Ser Phe Met 
    1115                 1120                 1125             


Ala Leu  Met Ser Leu Met Leu  Gln Met Arg Asn Ser  Ile Thr Gly 
    1130                 1135                 1140             


Arg Thr  Asp Val Asp Phe Leu  Ile Ser Pro Val Lys  Asn Ser Asp 
    1145                 1150                 1155             


Gly Ile  Phe Tyr Asp Ser Arg  Asn Tyr Glu Ala Gln  Glu Asn Ala 
    1160                 1165                 1170             


Ile Leu  Pro Lys Asn Ala Asp  Ala Asn Gly Ala Tyr  Asn Ile Ala 
    1175                 1180                 1185             


Arg Lys  Val Leu Trp Ala Ile  Gly Gln Phe Lys Lys  Ala Glu Asp 
    1190                 1195                 1200             


Glu Lys  Leu Asp Lys Val Lys  Ile Ala Ile Ser Asn  Lys Glu Trp 
    1205                 1210                 1215             


Leu Glu  Tyr Ala Gln Thr Ser  Val Lys His 
    1220                 1225             


<210>  45
<211>  1228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LbCpf1 RVR

<400>  45

Met Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr Gln Glu Asn Ile Asp 
            20                  25                  30          


Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr Lys 
        35                  40                  45              


Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn Asp 
    50                  55                  60                  


Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser Leu 
65                  70                  75                  80  


Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu Asn 
                85                  90                  95      


Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly Asn 
            100                 105                 110         


Glu Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile Leu 
        115                 120                 125             


Pro Glu Phe Leu Asp Asp Lys Asp Glu Ile Ala Leu Val Asn Ser Phe 
    130                 135                 140                 


Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu Asn 
145                 150                 155                 160 


Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys Ile 
                165                 170                 175     


Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu Lys 
            180                 185                 190         


Val Asp Ala Ile Phe Asp Lys His Glu Val Gln Glu Ile Lys Glu Lys 
        195                 200                 205             


Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu Phe 
    210                 215                 220                 


Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala Ile 
225                 230                 235                 240 


Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu Asn 
                245                 250                 255     


Glu Tyr Ile Asn Leu Tyr Asn Gln Lys Thr Lys Gln Lys Leu Pro Lys 
            260                 265                 270         


Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp Arg Glu Ser Leu Ser 
        275                 280                 285             


Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu Val Leu Glu Val Phe 
    290                 295                 300                 


Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys Lys 
305                 310                 315                 320 


Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly Ile 
                325                 330                 335     


Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile Phe 
            340                 345                 350         


Gly Glu Trp Asn Val Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp Asp 
        355                 360                 365             


Ile His Leu Lys Lys Lys Ala Val Val Thr Glu Lys Tyr Glu Asp Asp 
    370                 375                 380                 


Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln Leu 
385                 390                 395                 400 


Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val Glu Lys Leu Lys Glu 
                405                 410                 415     


Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser Ser 
            420                 425                 430         


Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu Lys Ser Leu Lys Lys 
        435                 440                 445             


Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu Leu Asp Ser Val Lys 
    450                 455                 460                 


Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu Thr 
465                 470                 475                 480 


Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp Ile 
                485                 490                 495     


Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile Arg Asn Tyr Val Thr 
            500                 505                 510         


Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn Pro 
        515                 520                 525             


Gln Phe Met Arg Gly Trp Asp Lys Asp Val Glu Thr Asp Arg Arg Ala 
    530                 535                 540                 


Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp Lys 
545                 550                 555                 560 


Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys Asp Asp Val Asn Gly 
                565                 570                 575     


Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys Met 
            580                 585                 590         


Leu Pro Lys Val Phe Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn Pro 
        595                 600                 605             


Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys Gly 
    610                 615                 620                 


Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu Ile Asp Phe Phe Lys 
625                 630                 635                 640 


Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe Asn 
                645                 650                 655     


Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg Glu 
            660                 665                 670         


Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys Lys 
        675                 680                 685             


Glu Val Asp Lys Leu Val Glu Glu Gly Lys Leu Tyr Met Phe Gln Ile 
    690                 695                 700                 


Tyr Asn Lys Asp Phe Ser Asp Lys Ser His Gly Thr Pro Asn Leu His 
705                 710                 715                 720 


Thr Met Tyr Phe Lys Leu Leu Phe Asp Glu Asn Asn His Gly Gln Ile 
                725                 730                 735     


Arg Leu Ser Gly Gly Ala Glu Leu Phe Met Arg Arg Ala Ser Leu Lys 
            740                 745                 750         


Lys Glu Glu Leu Val Val His Pro Ala Asn Ser Pro Ile Ala Asn Lys 
        755                 760                 765             


Asn Pro Asp Asn Pro Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val Tyr 
    770                 775                 780                 


Lys Asp Lys Arg Phe Ser Glu Asp Gln Tyr Glu Leu His Ile Pro Ile 
785                 790                 795                 800 


Ala Ile Asn Lys Cys Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu Val 
                805                 810                 815     


Arg Val Leu Leu Lys His Asp Asp Asn Pro Tyr Val Ile Gly Ile Asp 
            820                 825                 830         


Arg Gly Glu Arg Asn Leu Leu Tyr Ile Val Val Val Asp Gly Lys Gly 
        835                 840                 845             


Asn Ile Val Glu Gln Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe Asn 
    850                 855                 860                 


Gly Ile Arg Ile Lys Thr Asp Tyr His Ser Leu Leu Asp Lys Lys Glu 
865                 870                 875                 880 


Lys Glu Arg Phe Glu Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn Ile 
                885                 890                 895     


Lys Glu Leu Lys Ala Gly Tyr Ile Ser Gln Val Val His Lys Ile Cys 
            900                 905                 910         


Glu Leu Val Glu Lys Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu Asn 
        915                 920                 925             


Ser Gly Phe Lys Asn Ser Arg Val Lys Val Glu Lys Gln Val Tyr Gln 
    930                 935                 940                 


Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Met Val Asp Lys 
945                 950                 955                 960 


Lys Ser Asn Pro Cys Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln Ile 
                965                 970                 975     


Thr Asn Lys Phe Glu Ser Phe Lys Ser Met Ser Thr Gln Asn Gly Phe 
            980                 985                 990         


Ile Phe Tyr Ile Pro Ala Trp Leu  Thr Ser Lys Ile Asp  Pro Ser Thr 
        995                 1000                 1005             


Gly Phe  Val Asn Leu Leu Lys  Thr Lys Tyr Thr Ser  Ile Ala Asp 
    1010                 1015                 1020             


Ser Lys  Lys Phe Ile Ser Ser  Phe Asp Arg Ile Met  Tyr Val Pro 
    1025                 1030                 1035             


Glu Glu  Asp Leu Phe Glu Phe  Ala Leu Asp Tyr Lys  Asn Phe Ser 
    1040                 1045                 1050             


Arg Thr  Asp Ala Asp Tyr Ile  Lys Lys Trp Lys Leu  Tyr Ser Tyr 
    1055                 1060                 1065             


Gly Asn  Arg Ile Arg Ile Phe  Arg Asn Pro Lys Lys  Asn Asn Val 
    1070                 1075                 1080             


Phe Asp  Trp Glu Glu Val Cys  Leu Thr Ser Ala Tyr  Lys Glu Leu 
    1085                 1090                 1095             


Phe Asn  Lys Tyr Gly Ile Asn  Tyr Gln Gln Gly Asp  Ile Arg Ala 
    1100                 1105                 1110             


Leu Leu  Cys Glu Gln Ser Asp  Lys Ala Phe Tyr Ser  Ser Phe Met 
    1115                 1120                 1125             


Ala Leu  Met Ser Leu Met Leu  Gln Met Arg Asn Ser  Ile Thr Gly 
    1130                 1135                 1140             


Arg Thr  Asp Val Asp Phe Leu  Ile Ser Pro Val Lys  Asn Ser Asp 
    1145                 1150                 1155             


Gly Ile  Phe Tyr Asp Ser Arg  Asn Tyr Glu Ala Gln  Glu Asn Ala 
    1160                 1165                 1170             


Ile Leu  Pro Lys Asn Ala Asp  Ala Asn Gly Ala Tyr  Asn Ile Ala 
    1175                 1180                 1185             


Arg Lys  Val Leu Trp Ala Ile  Gly Gln Phe Lys Lys  Ala Glu Asp 
    1190                 1195                 1200             


Glu Lys  Leu Asp Lys Val Lys  Ile Ala Ile Ser Asn  Lys Glu Trp 
    1205                 1210                 1215             


Leu Glu  Tyr Ala Gln Thr Ser  Val Lys His 
    1220                 1225             


<210>  46
<211>  1307
<212>  PRT
<213>  Acidaminococcus

<400>  46

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  47
<211>  1307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AsCpf1 RR

<400>  47

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Arg Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Arg Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  48
<211>  1307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AsCpf1 RVR

<400>  48

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Arg Gly Trp 
    530                 535                 540                 


Asp Val Asn Val Glu Lys Asn Arg Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  49
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Partial MAD7 scaffold sequence

<400>  49
aatttctact cttgtagat                                                    19


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMG13 guide RNA sequence 1

<400>  50
ctcgtcacga ttcccctctc c                                                 21


<210>  51
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMG13 guide RNA sequence 2

<400>  51
tgtgtggtca cacttgccag c                                                 21


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZmCPL3 guide RNA sequence 1

<400>  52
gtcactgctg ccgggggcgg g                                                 21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZmCPL3 guide RNA sequence 2

<400>  53
aggtgtctga gaaaaccagt t                                                 21


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZmCPL1 guide RNA sequence 1

<400>  54
gaggacagaa ttgatgcact t                                                 21


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ZmCPL1 guide RNA sequence 2

<400>  55
ggtggagtac aggtcaacat t                                                 21


<210>  56
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBP2 regeneration booster

<400>  56

Met Glu Ser Gly Ser Gly Thr Ala Ala Gly Ser Gly Tyr Val Tyr Arg 
1               5                   10                  15      


Gln Ser Gly Ser Thr Arg Trp Asn Pro Thr Ala Glu Gln Leu Ser Leu 
            20                  25                  30          


Leu Lys Glu Leu Tyr Tyr Arg Asn Gly Ile Arg Thr Pro Ser Ala Asp 
        35                  40                  45              


Gln Ile Arg Gln Ile Ser Ala Arg Leu Ser Arg Tyr Gly Lys Ile Glu 
    50                  55                  60                  


Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg 
65                  70                  75                  80  


Gln Lys Lys Arg Leu Ser Thr Val Gly Cys Asp Pro Ala Leu Ile Glu 
                85                  90                  95      


Met Gly Asn Val Ala Ser Leu Glu Phe Gly Thr Glu Ser Ala Leu Glu 
            100                 105                 110         


Ser Leu Ser Ser Gly Pro Ser Ser Glu Leu Arg Glu Ala Pro Thr Arg 
        115                 120                 125             


Lys Phe Tyr Glu Lys Lys Thr Val Gly Glu Asn Ser Thr Ile Ile Asn 
    130                 135                 140                 


Pro Val Glu Gln Asn Cys Thr Leu Ser Cys Gly Thr Ser Gln Glu Phe 
145                 150                 155                 160 


Gln Tyr Ala Val Asp Ser Arg Arg Val Met Lys Ala Met Glu Glu Lys 
                165                 170                 175     


Gln Ala Thr Asp Asp Glu Pro Asp Gly Asn Lys Trp Thr Glu Ser Asn 
            180                 185                 190         


Arg His Val Lys Thr Leu Pro Leu Phe Pro Leu His Asn Asn Glu Asp 
        195                 200                 205             


Gln Thr Leu Ile Lys Ser Asp Lys Glu Ile Tyr Cys Leu Gly Ser Cys 
    210                 215                 220                 


Glu Lys Lys Met Asp Leu Ser Pro Leu Gly His Ser Gly Ser Gln Arg 
225                 230                 235                 240 


Ala Ser Ala Leu Asp Leu Cys Leu Ser Leu Gly Asn Glu Ser Cys Gly 
                245                 250                 255     


Leu His Asp Asn 
            260 


<210>  57
<211>  783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBP2 regeneration booster coding sequence

<400>  57
atggaatcgg gctccggcac ggcggcaggg tctggttatg tctatcggca gagcggaagc       60

acccggtgga atccaacagc agaacagttg tcgctgctca aggaacttta ttaccggaat      120

ggaattcgga caccgtcggc agatcaaatt aggcaaattt cggcccggct gtccagatac      180

ggcaaaatag aagggaaaaa cgtcttttac tggtttcaaa atcataaagc acgggaacgg      240

cagaagaaaa gactttccac ggtcggctgc gaccctgctc tcatagaaat gggtaacgtc      300

gcgagcttgg aatttgggac cgaaagcgct cttgaatctc tcagctcagg cccgtccagc      360

gagttgcgcg aggctcctac ccgcaagttt tatgagaaga aaaccgttgg tgagaacagc      420

accataatca atcctgttga gcagaactgc acactttctt gcggtacttc gcaggaattt      480

cagtatgctg ttgatagccg ccgggtgatg aaggcaatgg aagagaagca agcaacggat      540

gatgaaccgg acggaaacaa atggacggag tcgaacaggc atgtgaagac cctccctctt      600

ttccccttgc ataataatga agatcagacc ttgatcaagt cggacaagga aatttattgc      660

cttgggagct gtgaaaaaaa aatggatctg tccccattgg gacactcggg ctctcagagg      720

gcgtcggcac tggatttgtg cctgtctttg ggtaatgaat cttgtggcct ccacgacaat      780

tga                                                                    783


<210>  58
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 1 from Figure 9B

<400>  58
gtaccaggcc accagctaac c                                                 21


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 2 from Figure 9B

<400>  59
gctgaaggct taagagcatg g                                                 21


<210>  60
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 3 from Figure 9B

<400>  60
gatcacactc tcctcaattc t                                                 21


<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 4 from Figure 9B

<400>  61
aggacccagc catgctctta a                                                 21


<210>  62
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 5 from Figure 9B

<400>  62
taggccacaa gcaacatcgc a                                                 21


<210>  63
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 6 from Figure 9B

<400>  63
gtacacggtc atcgctgtca a                                                 21


<210>  64
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 7 from Figure 9B

<400>  64
ctcgtaccga cagaagtatg g                                                 21


<210>  65
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 8 from Figure 9B

<400>  65
agagcatggc tgggtcctga a                                                 21


<210>  66
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 9 from Figure 9B

<400>  66
gccttcctgg accatcactt c                                                 21


<210>  67
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 10 from Figure 9B

<400>  67
ttgtggggcc agactctcac a                                                 21


<210>  68
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 11 from Figure 9B

<400>  68
attggagggg aagtggactg t                                                 21


<210>  69
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 12 from Figure 9B

<400>  69
agactactgt gttgccatct c                                                 21


<210>  70
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 13 from Figure 9B

<400>  70
catatgatgg aatcggaaca g                                                 21


<210>  71
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 14 from Figure 9B

<400>  71
gggggtgttg tatcagataa t                                                 21


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence 15 from Figure 9B

<400>  72
caagcccaac cccatctgat g                                                 21


