                               SEQUENCE LISTING

<110> NORTHEASTERN UNIVERSITY
 
<120> ENGINEERING BACILLUS SUBTILIS AS A VERSATILE AND STABLE PLATFORM 
      FOR PRODUCTION OF NANOBODIES

<130> NEX-05825

<140> PCT/US2020/055355
<141> 2020-10-13

<150> 62/914,130
<151> 2019-10-11

<160> 15    

<170> PatentIn version 3.5

<210> 1
<211> 381
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 1
tcccaagtac aactggtaga aagcggtggt ggtctggtac aagcaggtgg tagcctgcgt       60

ctgagctgcg cagcatcccg tcgttcctcc cgttcctggg ctatggcttg gttccgtcaa      120

gcaccaggta aagaacgtga atttgtggcg aaaattagcg gcgatggccg tctgaccacc      180

tatggagaca gcgtaaaagg tcgtttcacc atcagccgtg acaacgcgga atatctggtg      240

tatctgcaga tggacagcct gaaaccagaa gacaccgcag tttactactg cgctgctgat      300

gataactatg tgaccgcgag ctggcgtagc ggcccggatt attggggcca gggcacccaa      360

gtaaccgtaa gcagctaata g                                                381


<210> 2
<211> 363
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 2
tcccaagtac aactggtaga aagcggtggt ggtctggtac aagcaggtgg tagcctgcgt       60

ctgagctgca ccgcttccgg tcgtaccggt accatctact ccatggcttg gttccgtcaa      120

gcaccaggta aagaacgtga attcctggct accgttggtt ggtcctccgg tatcacctac      180

tacatggaca gcgtaaaagg tcgtttcacc atcagccgtg acaaaggtaa aaacaccgta      240

tacctgcaga tggacagcct gaaaccagaa gacaccgcag tttactactg caccgctacc      300

cgtgcttact ccgttggtta cgactactgg ggtcaaggca cccaagtaac cgtaagcagc      360

taa                                                                    363


<210> 3
<211> 130
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Ala Gln Val Gln Leu Val Glu Thr Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser 
            20                  25                  30          


Met His Ala Met Thr Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu 
        35                  40                  45              


Leu Val Ala Val Ile Thr Ser His Gly Asp Arg Ala Asn Tyr Thr Asp 
    50                  55                  60                  


Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Met 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Asn Val Pro Arg Tyr Asp Ser Trp Gly Gln Gly Thr Gln Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Leu Pro Glu Thr Gly Gly His His His His 
        115                 120                 125             


His His 
    130 


<210> 4
<211> 129
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met Ala Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Thr Ile Ser 
            20                  25                  30          


Ser Val Ala Val Gly Trp Tyr Arg Gln Thr Pro Gly Asn Gln Arg Glu 
        35                  40                  45              


Trp Val Ala Thr Ser Ser Thr Ser Ser Thr Thr Ala Thr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr 
65                  70                  75                  80  


Ile Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Lys Thr Gly Leu Thr Asn Trp Gly Arg Gly Thr Gln Val Thr 
            100                 105                 110         


Val Ser Ser Gly Gly Leu Pro Glu Thr Gly Gly His His His His His 
        115                 120                 125             


His 
    


<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 5
His His His His His His 
1               5       


<210> 6
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
tacaaaaaca tcagccgtag gatcccatca tcatcatcat cacagc                      46


<210> 7
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
gtacttggga tccctggaag tacaggtttt c                                      31


<210> 8
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
cttgaagtcc tctttcaggg acccagcgtt tcggaaattc aattg                       45


<210> 9
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
ctgccccggg gacgtcgact ctagactatt agaaattgtg cacatcc                     47


<210> 10
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
cttccaggga tcccaagtac aactggtag                                         29


<210> 11
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
ctgccccggg gacgtcgact ctagactatt agctgcttac ggttac                      46


<210> 12
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
cttgaagtcc tctttcaggg acccacgccg ttgggccccg cc                          42


<210> 13
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
ctgccccggg gacgtcgact ctagactatt acggctgtgc aagatgacgc aa               52


<210> 14
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
acaaaaacat cagccgtagg a                                                 21


<210> 15
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
ccggggacgt cgactctag                                                    19


