                         SEQUENCE LISTING

<110>  Coherus Biosciences, Inc.
 
<120>  METHODS OF PURIFYING RANIBIZUMAB OR A RANIBIZUMAB VARIANT

<130>  40299-0080WO1

<150>  62/914,307
<151>  2019-10-11

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  110
<212>  PRT
<213>  Artificial

<220>
<223>  Ranibizumab Light Chain

<400>  1

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val 
            100                 105                 110 


<210>  2
<211>  158
<212>  PRT
<213>  Artificial

<220>
<223>  Ranibizumab Heavy Chain

<400>  2

Ser Gly Gly Gly Ser Gly Ser Gly Asp Phe Asp Tyr Glu Lys Met Ala 
1               5                   10                  15      


Asn Ala Asn Lys Gly Ala Met Thr Glu Asn Ala Asp Glu Asn Ala Leu 
            20                  25                  30          


Gln Ser Asp Ala Lys Gly Lys Leu Asp Ser Val Ala Thr Asp Tyr Gly 
        35                  40                  45              


Ala Ala Ile Asp Gly Phe Ile Gly Asp Val Ser Gly Leu Ala Asn Gly 
    50                  55                  60                  


Asn Gly Ala Thr Gly Asp Phe Ala Gly Ser Asn Ser Gln Met Ala Gln 
65                  70                  75                  80  


Val Gly Asp Gly Asp Asn Ser Pro Leu Met Asn Asn Phe Arg Gln Tyr 
                85                  90                  95      


Leu Pro Ser Leu Pro Gln Ser Val Glu Cys Arg Pro Phe Val Phe Ser 
            100                 105                 110         


Ala Gly Lys Pro Tyr Glu Phe Ser Ile Asp Cys Asp Lys Ile Asn Leu 
        115                 120                 125             


Phe Arg Gly Val Phe Ala Phe Leu Leu Tyr Val Ala Thr Phe Met Tyr 
    130                 135                 140                 


Val Phe Ser Thr Phe Ala Asn Ile Leu Arg Asn Lys Glu Ser 
145                 150                 155             


<210>  3
<211>  214
<212>  PRT
<213>  Artificial

<220>
<223>  Ranibizumab Light Chain

<400>  3

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  4
<211>  235
<212>  PRT
<213>  Artificial

<220>
<223>  Ranibizumab Heavy Chain

<400>  4

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Asp Phe Thr His Tyr 
            20                  25                  30          


Gly Met Glu Thr Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala 
    50                  55                  60                  


Asp Phe Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Glu Thr Asn Ser Leu Arg Ala Glu Asp Thr Ala 
                85                  90                  95      


Val Tyr Tyr Cys Ala Lys Tyr Pro Tyr Tyr Tyr Gly Thr Ser His Trp 
            100                 105                 110         


Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
        115                 120                 125             


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
    130                 135                 140                 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
145                 150                 155                 160 


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
                165                 170                 175     


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
            180                 185                 190         


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
        195                 200                 205             


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
    210                 215                 220                 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Leu 
225                 230                 235 


<210>  5
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  5

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  6
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  6

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  7
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  7

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210>  8
<211>  28
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  8

Met Phe Lys Phe Lys Lys Lys Phe Leu Val Gly Leu Thr Ala Ala Phe 
1               5                   10                  15      


Met Ser Ile Ser Met Phe Ser Ala Thr Ala Ser Ala 
            20                  25              


<210>  9
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  9

Met Lys Gln Ser Thr Ala Ile Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      


Pro Val Thr Lys Ala 
            20      


<210>  10
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  10

Met Met Lys Arg Asn Ile Leu Ala Val Ile Val Pro Ala Leu Leu Val 
1               5                   10                  15      


Ala Gly Thr Ala Asn Ala 
            20          


<210>  11
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  11

Met Lys Lys Ser Thr Leu Ala Leu Val Val Met Gly Ile Val Ala Ser 
1               5                   10                  15      


Ala Ser Val Gln Ala 
            20      


<210>  12
<211>  26
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  12

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  13
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  13

Met Lys Val Lys Val Leu Ser Leu Leu Val Pro Ala Leu Leu Val Ala 
1               5                   10                  15      


Gly Ala Ala Asn Ala 
            20      


<210>  14
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  14

Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr 
1               5                   10                  15      


Leu Leu Ala Gly 
            20  


<210>  15
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  15

Met Met Ile Thr Leu Arg Lys Leu Pro Leu Ala Val Ala Val Ala Ala 
1               5                   10                  15      


Gly Val Met Ser Ala Gln Ala Met Ala 
            20                  25  


<210>  16
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  16

Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile 
1               5                   10                  15      


Ser Ser Phe Ala 
            20  


<210>  17
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  17

Met Ser Ile Gln His Phe Arg Val Ala Leu Ile Pro Phe Phe Ala Ala 
1               5                   10                  15      


Phe Cys Leu Pro Val Phe Ala 
            20              


<210>  18
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  18

Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      


Pro Val Thr Lys Ala 
            20      


<210>  19
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  19

Met Lys Val Met Arg Thr Thr Val Ala Thr Val Val Ala Ala Thr Leu 
1               5                   10                  15      


Ser Met Ser Ala Phe Ser Val Phe Ala 
            20                  25  


<210>  20
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  Signal Sequence

<400>  20

Met Lys Lys Ser Leu Val Leu Lys Ala Ser Val Ala Val Ala Thr Leu 
1               5                   10                  15      


Val Pro Met Leu Ser Phe Ala 
            20              


