                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  SUSTAINABLE PRODUCTION OF CANNABINOIDS FROM SIMPLE PRECURSOR 
       FEEDSTOCKS USING SACCHAROMYCES CEREVISIAE

<130>  218005-0109PCT

<150>  US 62/914,058
<151>  2019-10-11

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  542
<212>  PRT
<213>  Nicotiana tabacum


<220>
<221>  misc_feature
<223>  4-coumarate-CoA ligase 2

<400>  1

Met Glu Lys Asp Thr Lys Gln Val Asp Ile Ile Phe Arg Ser Lys Leu 
1               5                   10                  15      


Pro Asp Ile Tyr Ile Pro Asn His Leu Pro Leu His Ser Tyr Cys Phe 
            20                  25                  30          


Glu Asn Ile Ser Glu Phe Ser Ser Arg Pro Cys Leu Ile Asn Gly Ala 
        35                  40                  45              


Asn Lys Gln Ile Tyr Thr Tyr Ala Asp Val Glu Leu Asn Ser Arg Lys 
    50                  55                  60                  


Val Ala Ala Gly Leu His Lys Gln Gly Ile Gln Pro Lys Asp Thr Ile 
65                  70                  75                  80  


Met Ile Leu Leu Pro Asn Ser Pro Glu Phe Val Phe Ala Phe Ile Gly 
                85                  90                  95      


Ala Ser Tyr Leu Gly Ala Ile Ser Thr Met Ala Asn Pro Leu Phe Thr 
            100                 105                 110         


Pro Ala Glu Val Val Lys Gln Ala Lys Ala Ser Ser Ala Lys Ile Ile 
        115                 120                 125             


Val Thr Gln Ala Cys His Val Asn Lys Val Lys Asp Tyr Ala Phe Glu 
    130                 135                 140                 


Asn Asp Val Lys Ile Ile Cys Ile Asp Ser Ala Pro Glu Gly Cys Leu 
145                 150                 155                 160 


His Phe Ser Val Leu Thr Gln Ala Asn Glu His Asp Ile Pro Glu Val 
                165                 170                 175     


Glu Ile Gln Pro Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly Thr 
            180                 185                 190         


Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Lys Gly Leu Val Thr 
        195                 200                 205             


Ser Val Ala Gln Gln Val Asp Gly Glu Asn Pro Asn Leu Tyr Ile His 
    210                 215                 220                 


Ser Glu Asp Val Met Leu Cys Val Leu Pro Leu Phe His Ile Tyr Ser 
225                 230                 235                 240 


Leu Asn Ser Val Leu Leu Cys Gly Leu Arg Val Gly Ala Ala Ile Leu 
                245                 250                 255     


Ile Met Gln Lys Phe Asp Ile Val Ser Phe Leu Glu Leu Ile Gln Ser 
            260                 265                 270         


Tyr Lys Val Thr Ile Gly Pro Phe Val Pro Pro Ile Val Leu Ala Ile 
        275                 280                 285             


Ala Lys Ser Pro Met Val Asp Asp Tyr Asp Leu Ser Ser Val Arg Thr 
    290                 295                 300                 


Val Met Ser Gly Ala Ala Pro Leu Gly Lys Glu Leu Glu Asp Thr Val 
305                 310                 315                 320 


Arg Ala Lys Phe Pro Asn Ala Lys Leu Gly Gln Gly Tyr Gly Met Thr 
                325                 330                 335     


Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu Pro 
            340                 345                 350         


Phe Glu Ile Lys Ser Gly Ala Cys Gly Thr Val Val Arg Asn Ala Glu 
        355                 360                 365             


Met Lys Ile Val Asp Pro Lys Thr Gly Asn Ser Leu Pro Arg Asn Gln 
    370                 375                 380                 


Ser Gly Glu Ile Cys Ile Arg Gly Asp Gln Ile Met Lys Gly Tyr Leu 
385                 390                 395                 400 


Asn Asp Pro Glu Ala Thr Ala Arg Thr Ile Asp Lys Glu Gly Trp Leu 
                405                 410                 415     


Tyr Thr Gly Asp Ile Gly Tyr Ile Asp Asp Asp Asp Glu Leu Phe Ile 
            420                 425                 430         


Val Asp Arg Leu Lys Glu Leu Ile Lys Tyr Lys Gly Phe Gln Val Ala 
        435                 440                 445             


Pro Ala Glu Leu Glu Ala Leu Leu Leu Asn His Pro Asn Ile Ser Asp 
    450                 455                 460                 


Ala Ala Val Val Pro Met Lys Asp Glu Gln Ala Gly Glu Val Pro Val 
465                 470                 475                 480 


Ala Phe Val Val Arg Ser Asn Gly Ser Thr Ile Thr Glu Asp Glu Val 
                485                 490                 495     


Lys Asp Phe Ile Ser Lys Gln Val Ile Phe Tyr Lys Arg Ile Lys Arg 
            500                 505                 510         


Val Phe Phe Val Asp Ala Ile Pro Lys Ser Pro Ser Gly Lys Ile Leu 
        515                 520                 525             


Arg Lys Asp Leu Arg Ala Lys Leu Ala Ala Gly Leu Pro Asn 
    530                 535                 540         


<210>  2
<211>  524
<212>  PRT
<213>  Rhodopseudomonas palustri


<220>
<221>  misc_feature
<223>  benzoate-CoA ligase

<400>  2

Met Asn Ala Ala Ala Val Thr Pro Pro Pro Glu Lys Phe Asn Phe Ala 
1               5                   10                  15      


Glu His Leu Leu Gln Thr Asn Arg Val Arg Pro Asp Lys Thr Ala Phe 
            20                  25                  30          


Val Asp Asp Ile Ser Ser Leu Ser Phe Ala Gln Leu Glu Ala Gln Thr 
        35                  40                  45              


Arg Gln Leu Ala Ala Ala Leu Arg Ala Ile Gly Val Lys Arg Glu Glu 
    50                  55                  60                  


Arg Val Leu Leu Leu Met Leu Asp Gly Thr Asp Trp Pro Val Ala Phe 
65                  70                  75                  80  


Leu Gly Ala Ile Tyr Ala Gly Ile Val Pro Val Ala Val Asn Thr Leu 
                85                  90                  95      


Leu Thr Ala Asp Asp Tyr Ala Tyr Met Leu Glu His Ser Arg Ala Gln 
            100                 105                 110         


Ala Val Leu Val Ser Gly Ala Leu His Pro Val Leu Lys Ala Ala Leu 
        115                 120                 125             


Thr Lys Ser Asp His Glu Val Gln Arg Val Ile Val Ser Arg Pro Ala 
    130                 135                 140                 


Ala Pro Leu Glu Pro Gly Glu Val Asp Phe Ala Glu Phe Val Gly Ala 
145                 150                 155                 160 


His Ala Pro Leu Glu Lys Pro Ala Ala Thr Gln Ala Asp Asp Pro Ala 
                165                 170                 175     


Phe Trp Leu Tyr Ser Ser Gly Ser Thr Gly Arg Pro Lys Gly Val Val 
            180                 185                 190         


His Thr His Ala Asn Pro Tyr Trp Thr Ser Glu Leu Tyr Gly Arg Asn 
        195                 200                 205             


Thr Leu His Leu Arg Glu Asp Asp Val Cys Phe Ser Ala Ala Lys Leu 
    210                 215                 220                 


Phe Phe Ala Tyr Gly Leu Gly Asn Ala Leu Thr Phe Pro Met Thr Val 
225                 230                 235                 240 


Gly Ala Thr Thr Leu Leu Met Gly Glu Arg Pro Thr Pro Asp Ala Val 
                245                 250                 255     


Phe Lys Arg Trp Leu Gly Gly Val Gly Gly Val Lys Pro Thr Val Phe 
            260                 265                 270         


Tyr Gly Ala Pro Thr Gly Tyr Ala Gly Met Leu Ala Ala Pro Asn Leu 
        275                 280                 285             


Pro Ser Arg Asp Gln Val Ala Leu Arg Leu Ala Ser Ser Ala Gly Glu 
    290                 295                 300                 


Ala Leu Pro Ala Glu Ile Gly Gln Arg Phe Gln Arg His Phe Gly Leu 
305                 310                 315                 320 


Asp Ile Val Asp Gly Ile Gly Ser Thr Glu Met Leu His Ile Phe Leu 
                325                 330                 335     


Ser Asn Leu Pro Asp Arg Val Arg Tyr Gly Thr Thr Gly Trp Pro Val 
            340                 345                 350         


Pro Gly Tyr Gln Ile Glu Leu Arg Gly Asp Gly Gly Gly Pro Val Ala 
        355                 360                 365             


Asp Gly Glu Pro Gly Asp Leu Tyr Ile His Gly Pro Ser Ser Ala Thr 
    370                 375                 380                 


Met Tyr Trp Gly Asn Arg Ala Lys Ser Arg Asp Thr Phe Gln Gly Gly 
385                 390                 395                 400 


Trp Thr Lys Ser Gly Asp Lys Tyr Val Arg Asn Asp Asp Gly Ser Tyr 
                405                 410                 415     


Thr Tyr Ala Gly Arg Thr Asp Asp Met Leu Lys Val Ser Gly Ile Tyr 
            420                 425                 430         


Val Ser Pro Phe Glu Ile Glu Ala Thr Leu Val Gln His Pro Gly Val 
        435                 440                 445             


Leu Glu Ala Ala Val Val Gly Val Ala Asp Glu His Gly Leu Thr Lys 
    450                 455                 460                 


Pro Lys Ala Tyr Val Val Pro Arg Pro Gly Gln Thr Leu Ser Glu Thr 
465                 470                 475                 480 


Glu Leu Lys Thr Phe Ile Lys Asp Arg Leu Ala Pro Tyr Lys Tyr Pro 
                485                 490                 495     


Arg Ser Thr Val Phe Val Ala Glu Leu Pro Lys Thr Ala Thr Gly Lys 
            500                 505                 510         


Ile Gln Arg Phe Lys Leu Arg Glu Gly Val Leu Gly 
        515                 520                 


<210>  3
<211>  448
<212>  PRT
<213>  Streptomyces coelicolor


<220>
<221>  misc_feature
<223>  phenylacetate-CoA ligase

<400>  3

Met Ser Ser Glu Pro Thr Thr Gly Thr Ala Pro Ala Pro Arg Arg Gly 
1               5                   10                  15      


Glu Pro Leu Pro His Asp Leu Leu Asp Asp Ala Glu Arg Leu Ser Arg 
            20                  25                  30          


Glu Gln Leu Arg Glu Leu Gln Leu Asp Arg Leu Arg Ala Thr Leu Arg 
        35                  40                  45              


His Ala Tyr Asp Asn Val Glu Leu Tyr Arg Lys Lys Phe Asp Ala Ala 
    50                  55                  60                  


Gly Val Thr Pro Asp Asp Cys Arg Ser Leu Ala Asp Leu Ser Arg Phe 
65                  70                  75                  80  


Pro Phe Thr Thr Lys Ala Asp Leu Arg Asp Thr Tyr Pro Phe Gly Met 
                85                  90                  95      


Phe Ala Val Pro Met Ala Asp Val Arg Arg Val His Ala Ser Ser Gly 
            100                 105                 110         


Thr Thr Gly Arg Ala Thr Val Val Gly Tyr Thr Glu Asn Asp Leu Ser 
        115                 120                 125             


Met Trp Ala Asp Val Val Ala Arg Ser Ile Arg Ala Ala Gly Gly Arg 
    130                 135                 140                 


Pro Gly His Lys Val His Ile Ser Tyr Gly Tyr Gly Leu Phe Thr Gly 
145                 150                 155                 160 


Gly Leu Gly Ala His Tyr Gly Ala Glu Arg Ala Gly Cys Thr Val Ile 
                165                 170                 175     


Pro Ala Ser Gly Gly Met Thr Ala Arg Gln Val Gln Ile Ile Gln Asp 
            180                 185                 190         


Phe Arg Pro Glu Ile Ile Met Val Thr Pro Ser Tyr Met Leu Thr Leu 
        195                 200                 205             


Leu Asp Glu Phe Glu Arg Gln Gly Val Asp Pro Arg Thr Ser Ser Leu 
    210                 215                 220                 


Gln Val Gly Ile Phe Gly Ala Glu Pro Trp Thr Glu Glu Met Arg Arg 
225                 230                 235                 240 


Glu Ile Glu Glu Arg Met Asp Ile His Ala Val Asp Ile Tyr Gly Leu 
                245                 250                 255     


Ser Glu Val Ile Gly Pro Gly Val Ala Gln Glu Cys Val Glu Thr Lys 
            260                 265                 270         


Asp Gly Leu His Ile Trp Glu Asp His Phe Tyr Pro Glu Val Val Asp 
        275                 280                 285             


Pro Leu Thr Asp Ala Val Leu Pro Gly Gly Glu Glu Gly Glu Ile Val 
    290                 295                 300                 


Phe Thr Ser Leu Thr Lys Glu Ala Leu Pro Val Ile Arg Tyr Arg Thr 
305                 310                 315                 320 


Arg Asp Leu Thr Arg Leu Leu Pro Gly Thr Ala Arg Pro Ala Phe Arg 
                325                 330                 335     


Arg Met Arg Lys Val Thr Gly Arg Cys Asp Asp Met Ile Ile Leu Arg 
            340                 345                 350         


Gly Val Asn Val Phe Pro Thr Gln Val Glu Glu Ile Val Leu Arg Thr 
        355                 360                 365             


Pro Gly Val Ala Pro His Phe Gln Met Arg Leu Thr Glu Arg Gly Arg 
    370                 375                 380                 


Met Asp His Met Thr Val Arg Val Glu Ala Arg Pro Asp Ala Ala Pro 
385                 390                 395                 400 


Glu Gln Arg Asp Ala Ala Ala Arg Ala Ile Ala Gln Gly Val Lys Asp 
                405                 410                 415     


Gly Val Gly Val Thr Val Glu Val Glu Val Val Glu Pro Glu Thr Leu 
            420                 425                 430         


Glu Arg Ser Leu Gly Lys Ile Arg Arg Val Trp Asp Gln Arg Gly Ala 
        435                 440                 445             


<210>  4
<211>  543
<212>  PRT
<213>  Cannabis sativa


<220>
<221>  misc_feature
<223>  acyl-activating enzyme 3

<400>  4

Met Glu Lys Ser Gly Tyr Gly Arg Asp Gly Ile Tyr Arg Ser Leu Arg 
1               5                   10                  15      


Pro Pro Leu His Leu Pro Asn Asn Asn Asn Leu Ser Met Val Ser Phe 
            20                  25                  30          


Leu Phe Arg Asn Ser Ser Ser Tyr Pro Gln Lys Pro Ala Leu Ile Asp 
        35                  40                  45              


Ser Glu Thr Asn Gln Ile Leu Ser Phe Ser His Phe Lys Ser Thr Val 
    50                  55                  60                  


Ile Lys Val Ser His Gly Phe Leu Asn Leu Gly Ile Lys Lys Asn Asp 
65                  70                  75                  80  


Val Val Leu Ile Tyr Ala Pro Asn Ser Ile His Phe Pro Val Cys Phe 
                85                  90                  95      


Leu Gly Ile Ile Ala Ser Gly Ala Ile Ala Thr Thr Ser Asn Pro Leu 
            100                 105                 110         


Tyr Thr Val Ser Glu Leu Ser Lys Gln Val Lys Asp Ser Asn Pro Lys 
        115                 120                 125             


Leu Ile Ile Thr Val Pro Gln Leu Leu Glu Lys Val Lys Gly Phe Asn 
    130                 135                 140                 


Leu Pro Thr Ile Leu Ile Gly Pro Asp Ser Glu Gln Glu Ser Ser Ser 
145                 150                 155                 160 


Asp Lys Val Met Thr Phe Asn Asp Leu Val Asn Leu Gly Gly Ser Ser 
                165                 170                 175     


Gly Ser Glu Phe Pro Ile Val Asp Asp Phe Lys Gln Ser Asp Thr Ala 
            180                 185                 190         


Ala Leu Leu Tyr Ser Ser Gly Thr Thr Gly Met Ser Lys Gly Val Val 
        195                 200                 205             


Leu Thr His Lys Asn Phe Ile Ala Ser Ser Leu Met Val Thr Met Glu 
    210                 215                 220                 


Gln Asp Leu Val Gly Glu Met Asp Asn Val Phe Leu Cys Phe Leu Pro 
225                 230                 235                 240 


Met Phe His Val Phe Gly Leu Ala Ile Ile Thr Tyr Ala Gln Leu Gln 
                245                 250                 255     


Arg Gly Asn Thr Val Ile Ser Met Ala Arg Phe Asp Leu Glu Lys Met 
            260                 265                 270         


Leu Lys Asp Val Glu Lys Tyr Lys Val Thr His Leu Trp Val Val Pro 
        275                 280                 285             


Pro Val Ile Leu Ala Leu Ser Lys Asn Ser Met Val Lys Lys Phe Asn 
    290                 295                 300                 


Leu Ser Ser Ile Lys Tyr Ile Gly Ser Gly Ala Ala Pro Leu Gly Lys 
305                 310                 315                 320 


Asp Leu Met Glu Glu Cys Ser Lys Val Val Pro Tyr Gly Ile Val Ala 
                325                 330                 335     


Gln Gly Tyr Gly Met Thr Glu Thr Cys Gly Ile Val Ser Met Glu Asp 
            340                 345                 350         


Ile Arg Gly Gly Lys Arg Asn Ser Gly Ser Ala Gly Met Leu Ala Ser 
        355                 360                 365             


Gly Val Glu Ala Gln Ile Val Ser Val Asp Thr Leu Lys Pro Leu Pro 
    370                 375                 380                 


Pro Asn Gln Leu Gly Glu Ile Trp Val Lys Gly Pro Asn Met Met Gln 
385                 390                 395                 400 


Gly Tyr Phe Asn Asn Pro Gln Ala Thr Lys Leu Thr Ile Asp Lys Lys 
                405                 410                 415     


Gly Trp Val His Thr Gly Asp Leu Gly Tyr Phe Asp Glu Asp Gly His 
            420                 425                 430         


Leu Tyr Val Val Asp Arg Ile Lys Glu Leu Ile Lys Tyr Lys Gly Phe 
        435                 440                 445             


Gln Val Ala Pro Ala Glu Leu Glu Gly Leu Leu Val Ser His Pro Glu 
    450                 455                 460                 


Ile Leu Asp Ala Val Val Ile Pro Phe Pro Asp Ala Glu Ala Gly Glu 
465                 470                 475                 480 


Val Pro Val Ala Tyr Val Val Arg Ser Pro Asn Ser Ser Leu Thr Glu 
                485                 490                 495     


Asn Asp Val Lys Lys Phe Ile Ala Gly Gln Val Ala Ser Phe Lys Arg 
            500                 505                 510         


Leu Arg Lys Val Thr Phe Ile Asn Ser Val Pro Lys Ser Ala Ser Gly 
        515                 520                 525             


Lys Ile Leu Arg Arg Glu Leu Ile Gln Lys Val Arg Ser Asn Met 
    530                 535                 540             


<210>  5
<211>  504
<212>  PRT
<213>  Rhizobium trifolii


<220>
<221>  misc_feature
<223>  malonyl-CoA synthetase

<400>  5

Met Ser Asn His Leu Phe Asp Ala Met Arg Ala Ala Ala Pro Gly Asn 
1               5                   10                  15      


Ala Pro Phe Ile Arg Ile Asp Asn Thr Arg Thr Trp Thr Tyr Asp Asp 
            20                  25                  30          


Ala Phe Ala Leu Ser Gly Arg Ile Ala Ser Ala Met Asp Ala Leu Gly 
        35                  40                  45              


Ile Arg Pro Gly Asp Arg Val Ala Val Gln Val Glu Lys Ser Ala Glu 
    50                  55                  60                  


Ala Leu Ile Leu Tyr Leu Ala Cys Leu Arg Ser Gly Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Leu Asn Thr Ala Tyr Thr Leu Ala Glu Leu Asp Tyr Phe Ile Gly 
                85                  90                  95      


Asp Ala Glu Pro Arg Leu Val Val Val Ala Ser Ser Ala Arg Ala Gly 
            100                 105                 110         


Val Glu Thr Ile Ala Lys Pro Arg Gly Ala Ile Val Glu Thr Leu Asp 
        115                 120                 125             


Ala Ala Gly Ser Gly Ser Leu Leu Asp Leu Ala Arg Asp Glu Pro Ala 
    130                 135                 140                 


Asp Phe Val Asp Ala Ser Arg Ser Ala Asp Asp Leu Ala Ala Ile Leu 
145                 150                 155                 160 


Tyr Thr Ser Gly Thr Thr Gly Arg Ser Lys Gly Ala Met Leu Thr His 
                165                 170                 175     


Gly Asn Leu Leu Ser Asn Ala Leu Thr Leu Arg Asp Phe Trp Arg Val 
            180                 185                 190         


Thr Ala Gly Asp Arg Leu Ile His Ala Leu Pro Ile Phe His Thr His 
        195                 200                 205             


Gly Leu Phe Val Ala Thr Asn Val Thr Leu Leu Ala Gly Ala Ser Met 
    210                 215                 220                 


Phe Leu Leu Ser Lys Phe Asp Pro Glu Glu Ile Leu Ser Leu Met Pro 
225                 230                 235                 240 


Gln Ala Thr Met Leu Met Gly Val Pro Thr Phe Tyr Val Arg Leu Leu 
                245                 250                 255     


Gln Ser Pro Arg Leu Asp Lys Gln Ala Val Ala Asn Ile Arg Leu Phe 
            260                 265                 270         


Ile Ser Gly Ser Ala Pro Leu Leu Ala Glu Thr His Thr Glu Phe Gln 
        275                 280                 285             


Ala Arg Thr Gly His Ala Ile Leu Glu Arg Tyr Gly Met Thr Glu Thr 
    290                 295                 300                 


Asn Met Asn Thr Ser Asn Pro Tyr Glu Gly Lys Arg Ile Ala Gly Thr 
305                 310                 315                 320 


Val Gly Phe Pro Leu Pro Asp Val Thr Val Arg Val Thr Asp Pro Ala 
                325                 330                 335     


Thr Gly Leu Ala Leu Pro Pro Glu Gln Thr Gly Met Ile Glu Ile Lys 
            340                 345                 350         


Gly Pro Asn Val Phe Lys Gly Tyr Trp Arg Met Pro Glu Lys Thr Ala 
        355                 360                 365             


Ala Glu Phe Thr Ala Asp Gly Phe Phe Ile Ser Gly Asp Leu Gly Lys 
    370                 375                 380                 


Ile Asp Arg Asp Gly Tyr Val His Ile Val Gly Arg Gly Lys Asp Leu 
385                 390                 395                 400 


Val Ile Ser Gly Gly Tyr Asn Ile Tyr Pro Lys Glu Val Glu Gly Glu 
                405                 410                 415     


Ile Asp Gln Ile Glu Gly Val Val Glu Ser Ala Val Ile Gly Val Pro 
            420                 425                 430         


His Pro Asp Phe Gly Glu Gly Val Thr Ala Val Val Val Arg Lys Pro 
        435                 440                 445             


Gly Ala Ala Leu Asp Glu Lys Ala Ile Val Ser Ala Leu Gln Asp Arg 
    450                 455                 460                 


Leu Ala Arg Tyr Lys Gln Pro Lys Arg Ile Ile Phe Ala Glu Asp Leu 
465                 470                 475                 480 


Pro Arg Asn Thr Met Gly Lys Val Gln Lys Asn Ile Leu Arg Gln Gln 
                485                 490                 495     


Tyr Ala Asp Leu Tyr Thr Arg Thr 
            500                 


<210>  6
<211>  385
<212>  PRT
<213>  Cannabis sativa


<220>
<221>  misc_feature
<223>  olivetol synthase

<400>  6

Met Asn His Leu Arg Ala Glu Gly Pro Ala Ser Val Leu Ala Ile Gly 
1               5                   10                  15      


Thr Ala Asn Pro Glu Asn Ile Leu Leu Gln Asp Glu Phe Pro Asp Tyr 
            20                  25                  30          


Tyr Phe Arg Val Thr Lys Ser Glu His Met Thr Gln Leu Lys Glu Lys 
        35                  40                  45              


Phe Arg Lys Ile Cys Asp Lys Ser Met Ile Arg Lys Arg Asn Cys Phe 
    50                  55                  60                  


Leu Asn Glu Glu His Leu Lys Gln Asn Pro Arg Leu Val Glu His Glu 
65                  70                  75                  80  


Met Gln Thr Leu Asp Ala Arg Gln Asp Met Leu Val Val Glu Val Pro 
                85                  90                  95      


Lys Leu Gly Lys Asp Ala Cys Ala Lys Ala Ile Lys Glu Trp Gly Gln 
            100                 105                 110         


Pro Lys Ser Lys Ile Thr His Leu Ile Phe Thr Ser Ala Ser Thr Thr 
        115                 120                 125             


Asp Met Pro Gly Ala Asp Tyr His Cys Ala Lys Leu Leu Gly Leu Ser 
    130                 135                 140                 


Pro Ser Val Lys Arg Val Met Met Tyr Gln Leu Gly Cys Tyr Gly Gly 
145                 150                 155                 160 


Gly Thr Val Leu Arg Ile Ala Lys Asp Ile Ala Glu Asn Asn Lys Gly 
                165                 170                 175     


Ala Arg Val Leu Ala Val Cys Cys Asp Ile Met Ala Cys Leu Phe Arg 
            180                 185                 190         


Gly Pro Ser Glu Ser Asp Leu Glu Leu Leu Val Gly Gln Ala Ile Phe 
        195                 200                 205             


Gly Asp Gly Ala Ala Ala Val Ile Val Gly Ala Glu Pro Asp Glu Ser 
    210                 215                 220                 


Val Gly Glu Arg Pro Ile Phe Glu Leu Val Ser Thr Gly Gln Thr Ile 
225                 230                 235                 240 


Leu Pro Asn Ser Glu Gly Thr Ile Gly Gly His Ile Arg Glu Ala Gly 
                245                 250                 255     


Leu Ile Phe Asp Leu His Lys Asp Val Pro Met Leu Ile Ser Asn Asn 
            260                 265                 270         


Ile Glu Lys Cys Leu Ile Glu Ala Phe Thr Pro Ile Gly Ile Ser Asp 
        275                 280                 285             


Trp Asn Ser Ile Phe Trp Ile Thr His Pro Gly Gly Lys Ala Ile Leu 
    290                 295                 300                 


Asp Lys Val Glu Glu Lys Leu His Leu Lys Ser Asp Lys Phe Val Asp 
305                 310                 315                 320 


Ser Arg His Val Leu Ser Glu His Gly Asn Met Ser Ser Ser Thr Val 
                325                 330                 335     


Leu Phe Val Met Asp Glu Leu Arg Lys Arg Ser Leu Glu Glu Gly Lys 
            340                 345                 350         


Ser Thr Thr Gly Asp Gly Phe Glu Trp Gly Val Leu Phe Gly Phe Gly 
        355                 360                 365             


Pro Gly Leu Thr Val Glu Arg Val Val Val Arg Ser Val Pro Ile Lys 
    370                 375                 380                 


Tyr 
385 


<210>  7
<211>  101
<212>  PRT
<213>  Cannabis sativa


<220>
<221>  misc_feature
<223>  olivetolic acid cyclase

<400>  7

Met Ala Val Lys His Leu Ile Val Leu Lys Phe Lys Asp Glu Ile Thr 
1               5                   10                  15      


Glu Ala Gln Lys Glu Glu Phe Phe Lys Thr Tyr Val Asn Leu Val Asn 
            20                  25                  30          


Ile Ile Pro Ala Met Lys Asp Val Tyr Trp Gly Lys Asp Val Thr Gln 
        35                  40                  45              


Lys Asn Lys Glu Glu Gly Tyr Thr His Ile Val Glu Val Thr Phe Glu 
    50                  55                  60                  


Ser Val Glu Thr Ile Gln Asp Tyr Ile Ile His Pro Ala His Val Gly 
65                  70                  75                  80  


Phe Gly Asp Val Tyr Arg Ser Phe Trp Glu Lys Leu Leu Ile Phe Asp 
                85                  90                  95      


Tyr Thr Pro Arg Lys 
            100     


<210>  8
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Streptomyces sp. CL190 NphB V49W, Y288A

<400>  8

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Trp Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Ala 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  9
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Streptomyces sp. CL190 NphB V49W, Y288P

<400>  9

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Trp Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Pro 
        275                 280                 285             


Tyr His Ile Thr Asp Val Gln Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  10
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Streptomyces sp. CL190 NphB V49W, Y288A, Q295F

<400>  10

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Trp Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Ala 
        275                 280                 285             


Tyr His Ile Thr Asp Val Phe Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  11
<211>  307
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Streptomyces sp. CL190  NphB V49W, Y288P, Q295F

<400>  11

Met Ser Glu Ala Ala Asp Val Glu Arg Val Tyr Ala Ala Met Glu Glu 
1               5                   10                  15      


Ala Ala Gly Leu Leu Gly Val Ala Cys Ala Arg Asp Lys Ile Tyr Pro 
            20                  25                  30          


Leu Leu Ser Thr Phe Gln Asp Thr Leu Val Glu Gly Gly Ser Val Val 
        35                  40                  45              


Trp Phe Ser Met Ala Ser Gly Arg His Ser Thr Glu Leu Asp Phe Ser 
    50                  55                  60                  


Ile Ser Val Pro Thr Ser His Gly Asp Pro Tyr Ala Thr Val Val Glu 
65                  70                  75                  80  


Lys Gly Leu Phe Pro Ala Thr Gly His Pro Val Asp Asp Leu Leu Ala 
                85                  90                  95      


Asp Thr Gln Lys His Leu Pro Val Ser Met Phe Ala Ile Asp Gly Glu 
            100                 105                 110         


Val Thr Gly Gly Phe Lys Lys Thr Tyr Ala Phe Phe Pro Thr Asp Asn 
        115                 120                 125             


Met Pro Gly Val Ala Glu Leu Ser Ala Ile Pro Ser Met Pro Pro Ala 
    130                 135                 140                 


Val Ala Glu Asn Ala Glu Leu Phe Ala Arg Tyr Gly Leu Asp Lys Val 
145                 150                 155                 160 


Gln Met Thr Ser Met Asp Tyr Lys Lys Arg Gln Val Asn Leu Tyr Phe 
                165                 170                 175     


Ser Glu Leu Ser Ala Gln Thr Leu Glu Ala Glu Ser Val Leu Ala Leu 
            180                 185                 190         


Val Arg Glu Leu Gly Leu His Val Pro Asn Glu Leu Gly Leu Lys Phe 
        195                 200                 205             


Cys Lys Arg Ser Phe Ser Val Tyr Pro Thr Leu Asn Trp Glu Thr Gly 
    210                 215                 220                 


Lys Ile Asp Arg Leu Cys Phe Ala Val Ile Ser Asn Asp Pro Thr Leu 
225                 230                 235                 240 


Val Pro Ser Ser Asp Glu Gly Asp Ile Glu Lys Phe His Asn Tyr Ala 
                245                 250                 255     


Thr Lys Ala Pro Tyr Ala Tyr Val Gly Glu Lys Arg Thr Leu Val Tyr 
            260                 265                 270         


Gly Leu Thr Leu Ser Pro Lys Glu Glu Tyr Tyr Lys Leu Gly Ala Pro 
        275                 280                 285             


Tyr His Ile Thr Asp Val Phe Arg Gly Leu Leu Lys Ala Phe Asp Ser 
    290                 295                 300                 


Leu Glu Asp 
305         


<210>  12
<211>  538
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VSP-CBDA fusion

<400>  12

Met Phe Ser Leu Lys Ala Leu Leu Pro Leu Ala Leu Leu Leu Val Ser 
1               5                   10                  15      


Ala Asn Gln Val Ala Ala Asn Pro Arg Glu Asn Phe Leu Lys Cys Phe 
            20                  25                  30          


Ser Gln Tyr Ile Pro Asn Asn Ala Thr Asn Leu Lys Leu Val Tyr Thr 
        35                  40                  45              


Gln Asn Asn Pro Leu Tyr Met Ser Val Leu Asn Ser Thr Ile His Asn 
    50                  55                  60                  


Leu Arg Phe Thr Ser Asp Thr Thr Pro Lys Pro Leu Val Ile Val Thr 
65                  70                  75                  80  


Pro Ser His Val Ser His Ile Gln Gly Thr Ile Leu Cys Ser Lys Lys 
                85                  90                  95      


Val Gly Leu Gln Ile Arg Thr Arg Ser Gly Gly His Asp Ser Glu Gly 
            100                 105                 110         


Met Ser Tyr Ile Ser Gln Val Pro Phe Val Ile Val Asp Leu Arg Asn 
        115                 120                 125             


Met Arg Ser Ile Lys Ile Asp Val His Ser Gln Thr Ala Trp Val Glu 
    130                 135                 140                 


Ala Gly Ala Thr Leu Gly Glu Val Tyr Tyr Trp Val Asn Glu Lys Asn 
145                 150                 155                 160 


Glu Asn Leu Ser Leu Ala Ala Gly Tyr Cys Pro Thr Val Cys Ala Gly 
                165                 170                 175     


Gly His Phe Gly Gly Gly Gly Tyr Gly Pro Leu Met Arg Asn Tyr Gly 
            180                 185                 190         


Leu Ala Ala Asp Asn Ile Ile Asp Ala His Leu Val Asn Val His Gly 
        195                 200                 205             


Lys Val Leu Asp Arg Lys Ser Met Gly Glu Asp Leu Phe Trp Ala Leu 
    210                 215                 220                 


Arg Gly Gly Gly Ala Glu Ser Phe Gly Ile Ile Val Ala Trp Lys Ile 
225                 230                 235                 240 


Arg Leu Val Ala Val Pro Lys Ser Thr Met Phe Ser Val Lys Lys Ile 
                245                 250                 255     


Met Glu Ile His Glu Leu Val Lys Leu Val Asn Lys Trp Gln Asn Ile 
            260                 265                 270         


Ala Tyr Lys Tyr Asp Lys Asp Leu Leu Leu Met Thr His Phe Ile Thr 
        275                 280                 285             


Arg Asn Ile Thr Asp Asn Gln Gly Lys Asn Lys Thr Ala Ile His Thr 
    290                 295                 300                 


Tyr Phe Ser Ser Val Phe Leu Gly Gly Val Asp Ser Leu Val Asp Leu 
305                 310                 315                 320 


Met Asn Lys Ser Phe Pro Glu Leu Gly Ile Lys Lys Thr Asp Cys Arg 
                325                 330                 335     


Gln Leu Ser Trp Ile Asp Thr Ile Ile Phe Tyr Ser Gly Val Val Asn 
            340                 345                 350         


Tyr Asp Thr Asp Asn Phe Asn Lys Glu Ile Leu Leu Asp Arg Ser Ala 
        355                 360                 365             


Gly Gln Asn Gly Ala Phe Lys Ile Lys Leu Asp Tyr Val Lys Lys Pro 
    370                 375                 380                 


Ile Pro Glu Ser Val Phe Val Gln Ile Leu Glu Lys Leu Tyr Glu Glu 
385                 390                 395                 400 


Asp Ile Gly Ala Gly Met Tyr Ala Leu Tyr Pro Tyr Gly Gly Ile Met 
                405                 410                 415     


Asp Glu Ile Ser Glu Ser Ala Ile Pro Phe Pro His Arg Ala Gly Ile 
            420                 425                 430         


Leu Tyr Glu Leu Trp Tyr Ile Cys Ser Trp Glu Lys Gln Glu Asp Asn 
        435                 440                 445             


Glu Lys His Leu Asn Trp Ile Arg Asn Ile Tyr Asn Phe Met Thr Pro 
    450                 455                 460                 


Tyr Val Ser Lys Asn Pro Arg Leu Ala Tyr Leu Asn Tyr Arg Asp Leu 
465                 470                 475                 480 


Asp Ile Gly Ile Asn Asp Pro Lys Asn Pro Asn Asn Tyr Thr Gln Ala 
                485                 490                 495     


Arg Ile Trp Gly Glu Lys Tyr Phe Gly Lys Asn Phe Asp Arg Leu Val 
            500                 505                 510         


Lys Val Lys Thr Leu Val Asp Pro Asn Asn Phe Phe Arg Asn Glu Gln 
        515                 520                 525             


Ser Ile Pro Pro Leu Pro Arg His Arg His 
    530                 535             


