                         SEQUENCE LISTING

<110>  National University Of Singapore
 
<120>  GENETICALLY MODIFIED T CELLS AND USES THEREOF

<130>  10104SG1209

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Extracellular domain of PD-1

<400>  1

Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr 
1               5                   10                  15      


Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe 
            20                  25                  30          


Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr 
        35                  40                  45              


Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu 
    50                  55                  60                  


Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu 
65                  70                  75                  80  


Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn 
                85                  90                  95      


Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala 
            100                 105                 110         


Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg 
        115                 120                 125             


Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly 
    130                 135                 140                 


Gln Phe Gln Thr Leu Val 
145                 150 


<210>  2
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extracellular domain of PD-1

<400>  2
ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc       60

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      120

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      180

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      240

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      300

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      360

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      420

aggccagccg gccagttcca aaccctggtg                                       450


<210>  3
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Extracellular domain of the NKG2D receptor

<400>  3

Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro 
1               5                   10                  15      


Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe 
            20                  25                  30          


Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln 
        35                  40                  45              


Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu 
    50                  55                  60                  


Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile Pro Thr 
65                  70                  75                  80  


Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu 
                85                  90                  95      


Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser 
            100                 105                 110         


Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile 
        115                 120                 125             


Cys Met Gln Arg Thr Val Ala Ser 
    130                 135     


<210>  4
<211>  408
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extracellular domain of the NKG2D receptor

<400>  4
ttcaaccaag aagttcaaat tcccttgacc gaaagttact gtggcccatg tcctaaaaac       60

tggatatgtt acaaaaataa ctgctaccaa ttttttgatg agagtaaaaa ctggtatgag      120

agccaggctt cttgtatgtc tcaaaatgcc agccttctga aagtatacag caaagaggac      180

caggatttac ttaaactggt gaagtcatat cattggatgg gactagtaca cattccaaca      240

aatggatctt ggcagtggga agatggctcc attctctcac ccaacctact aacaataatt      300

gaaatgcaga agggagactg tgcactctat gcctcgagct ttaaaggcta tatagaaaac      360

tgttcaactc caaatacgta catctgtatg caaaggactg tggctagc                   408


<210>  5
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD-L1 scFv

<400>  5

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Gly Phe Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Thr Ala Tyr Asn Gly Asn Thr Asn Tyr Ala Gln Lys Leu 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Tyr Phe Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
    130                 135                 140                 


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser 
145                 150                 155                 160 


Val Ser Ser Tyr Leu Val Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
                165                 170                 175     


Arg Leu Leu Ile Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala 
            180                 185                 190         


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
        195                 200                 205             


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser 
    210                 215                 220                 


Asn Trp Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ala 
225                 230                 235                 240 


Ser 
    


<210>  6
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD-L1 scFv

<400>  6
caggttcagc tggtgcagtc tggagctgag gtgaagaagc ctggggcctc agtgaaggtc       60

tcctgcaagg cttctggtta cacctttacc gactatggtt tcagctgggt gcgacaggcc      120

cctggacaag ggcttgagtg gatgggatgg atcaccgctt acaatggtaa cacaaactat      180

gcacagaagc tccagggcag agtcaccatg accacagaca catccacgag cacagtctac      240

atggagctga ggagcctgag atctgacgac acggccgtgt attactgtgc gagagactac      300

ttctacggta tggacgtctg gggccaaggg accacggtca ccgtctcctc aggtggaggc      360

ggttcaggcg gaggtggcag cggcggtggc gggtcggaaa ttgtgttgac acagtctcca      420

gccaccctgt ctttgtctcc aggggaaaga gccaccctct cctgcagggc cagtcagagt      480

gttagcagct acttagtctg gtaccaacag aaacctggcc aggctcccag gctcctcatc      540

tatgatgcat ccaacagggc cactggcatc ccagccaggt tcagtggcag tgggtctggg      600

acagacttca ctctcaccat cagcagccta gagcctgaag attttgcagt ttattactgt      660

cagcagcgta gcaactggcc tcggacgttc ggccaaggga ccaaggtgga aatcaaagct      720

agc                                                                    723


<210>  7
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH PD-L1 scFv

<400>  7

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Gly Phe Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Thr Ala Tyr Asn Gly Asn Thr Asn Tyr Ala Gln Lys Leu 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Tyr Phe Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  8
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH PD-L1 scFv

<400>  8
caggttcagc tggtgcagtc tggagctgag gtgaagaagc ctggggcctc agtgaaggtc       60

tcctgcaagg cttctggtta cacctttacc gactatggtt tcagctgggt gcgacaggcc      120

cctggacaag ggcttgagtg gatgggatgg atcaccgctt acaatggtaa cacaaactat      180

gcacagaagc tccagggcag agtcaccatg accacagaca catccacgag cacagtctac      240

atggagctga ggagcctgag atctgacgac acggccgtgt attactgtgc gagagactac      300

ttctacggta tggacgtctg gggccaaggg accacggtca ccgtctcctc a               351


<210>  9
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL PD-L1 scFv

<400>  9

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr 
            20                  25                  30          


Leu Val Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Arg 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  10
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL PD-L1 scFv

<400>  10
gaaattgtgt tgacacagtc tccagccacc ctgtctttgt ctccagggga aagagccacc       60

ctctcctgca gggccagtca gagtgttagc agctacttag tctggtacca acagaaacct      120

ggccaggctc ccaggctcct catctatgat gcatccaaca gggccactgg catcccagcc      180

aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcagcag cctagagcct      240

gaagattttg cagtttatta ctgtcagcag cgtagcaact ggcctcggac gttcggccaa      300

gggaccaagg tggaaatcaa a                                                321


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD-L1 scFv VH and VL linker

<400>  11

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  12
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD-L1 scFv VH and VL linker

<400>  12
ggtggaggcg gttcaggcgg aggtggcagc ggcggtggcg ggtcg                       45


<210>  13
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain of CAR

<400>  13

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Phe Trp Val Leu 
1               5                   10                  15      


Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val 
            20                  25                  30          


Ala Phe Ile Ile Phe Trp Val 
        35                  


<210>  14
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain of CAR

<400>  14
gaatctaaat atggcccccc ttgcccacca tgtcccttct gggttttggt agttgtcggc       60

ggggtgcttg cttgctattc attgttggtt acggtggctt ttataatttt ctgggta         117


<210>  15
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain of CCR

<400>  15

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
    50                  55                  60                  


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
65                  70                  75                  80  


His Arg Asn 
            


<210>  16
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain of CCR

<400>  16
ttcgtgccgg tcttcctgcc agcgaagccc accacgacgc cagcgccgcg accaccaaca       60

ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg      120

gcggggggcg cagtgcacac gagggggctg gacttcgcct gtgatatcta catctgggcg      180

cccttggccg ggacttgtgg ggtccttctc ctgtcactgg ttatcaccct ttactgcaac      240

cacaggaac                                                              249


<210>  17
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Intracellular signaling domain of CAR

<400>  17

Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala 
1               5                   10                  15      


Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu 
            20                  25                  30          


Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg 
        35                  40                  45              


Pro Tyr Tyr Lys 
    50          


<210>  18
<211>  156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Intracellular signaling domain of CAR

<400>  18
tacttcctgg gacgacttgt tcctcggggg cggggtgctg cagaagctgc aacgcgaaaa       60

caaagaatta ccgagactga gagtccgtat caagaactgc agggccaaag aagcgacgtt      120

tactccgatc tcaataccca acggccttac tacaaa                                156


<210>  19
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Co-stimulatory signaling domain of CCR

<400>  19

Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
1               5                   10                  15      


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            20                  25                  30          


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40                  45          


<210>  20
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Co-stimulatory signaling domain of CCR

<400>  20
cgtttctctg ttgttaaacg gggcagaaag aagctcctgt atatattcaa acaaccattt       60

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa      120

gaagaaggag gatgtgaact g                                                141


<210>  21
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  21

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gly Ala His Ala 
            20                  25      


<210>  22
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  22
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccaggcg cgcatgcc                                                     78


<210>  23
<211>  284
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR without signal peptide

<400>  23

Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro 
1               5                   10                  15      


Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe 
            20                  25                  30          


Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln 
        35                  40                  45              


Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu 
    50                  55                  60                  


Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile Pro Thr 
65                  70                  75                  80  


Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu 
                85                  90                  95      


Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser 
            100                 105                 110         


Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile 
        115                 120                 125             


Cys Met Gln Arg Thr Val Ala Ser Asn Trp Ser His Pro Gln Phe Glu 
    130                 135                 140                 


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn Trp Ser His Pro 
145                 150                 155                 160 


Gln Phe Glu Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asn Trp 
                165                 170                 175     


Ser His Pro Gln Phe Glu Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            180                 185                 190         


Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Phe Trp Val 
        195                 200                 205             


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
    210                 215                 220                 


Val Ala Phe Ile Ile Phe Trp Val Tyr Phe Leu Gly Arg Leu Val Pro 
225                 230                 235                 240 


Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr 
                245                 250                 255     


Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val 
            260                 265                 270         


Tyr Ser Asp Leu Asn Thr Gln Arg Pro Tyr Tyr Lys 
        275                 280                 


<210>  24
<211>  852
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR without signal peptide

<400>  24
ttcaaccaag aagttcaaat tcccttgacc gaaagttact gtggcccatg tcctaaaaac       60

tggatatgtt acaaaaataa ctgctaccaa ttttttgatg agagtaaaaa ctggtatgag      120

agccaggctt cttgtatgtc tcaaaatgcc agccttctga aagtatacag caaagaggac      180

caggatttac ttaaactggt gaagtcatat cattggatgg gactagtaca cattccaaca      240

aatggatctt ggcagtggga agatggctcc attctctcac ccaacctact aacaataatt      300

gaaatgcaga agggagactg tgcactctat gcctcgagct ttaaaggcta tatagaaaac      360

tgttcaactc caaatacgta catctgtatg caaaggactg tggctagcaa ctggtcacac      420

ccccagtttg agaaaggtgg gggagggtca ggtggtgggg ggtcaaattg gtctcatccc      480

cagttcgaga agggcggagg aggttcaggt gggggtggga gcaactggag ccaccctcaa      540

tttgaaaaag gaggtggagg gagtggaggt ggtggatctg aatctaaata tggcccccct      600

tgcccaccat gtcccttctg ggttttggta gttgtcggcg gggtgcttgc ttgctattca      660

ttgttggtta cggtggcttt tataattttc tgggtatact tcctgggacg acttgttcct      720

cgggggcggg gtgctgcaga agctgcaacg cgaaaacaaa gaattaccga gactgagagt      780

ccgtatcaag aactgcaggg ccaaagaagc gacgtttact ccgatctcaa tacccaacgg      840

ccttactaca aa                                                          852


<210>  25
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR with signal peptide

<400>  25

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gly Ala His Ala Phe Asn Gln Glu Val Gln 
            20                  25                  30          


Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro Cys Pro Lys Asn Trp Ile 
        35                  40                  45              


Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe Asp Glu Ser Lys Asn Trp 
    50                  55                  60                  


Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln Asn Ala Ser Leu Leu Lys 
65                  70                  75                  80  


Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu Lys Leu Val Lys Ser Tyr 
                85                  90                  95      


His Trp Met Gly Leu Val His Ile Pro Thr Asn Gly Ser Trp Gln Trp 
            100                 105                 110         


Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu Leu Thr Ile Ile Glu Met 
        115                 120                 125             


Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser Ser Phe Lys Gly Tyr Ile 
    130                 135                 140                 


Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile Cys Met Gln Arg Thr Val 
145                 150                 155                 160 


Ala Ser Asn Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Gly Ser 
                165                 170                 175     


Gly Gly Gly Gly Ser Asn Trp Ser His Pro Gln Phe Glu Lys Gly Gly 
            180                 185                 190         


Gly Gly Ser Gly Gly Gly Gly Ser Asn Trp Ser His Pro Gln Phe Glu 
        195                 200                 205             


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly 
    210                 215                 220                 


Pro Pro Cys Pro Pro Cys Pro Phe Trp Val Leu Val Val Val Gly Gly 
225                 230                 235                 240 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                245                 250                 255     


Trp Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala 
            260                 265                 270         


Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr 
        275                 280                 285             


Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr 
    290                 295                 300                 


Gln Arg Pro Tyr Tyr Lys 
305                 310 


<210>  26
<211>  930
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR with signal peptide

<400>  26
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccaggcg cgcatgcctt caaccaagaa gttcaaattc ccttgaccga aagttactgt      120

ggcccatgtc ctaaaaactg gatatgttac aaaaataact gctaccaatt ttttgatgag      180

agtaaaaact ggtatgagag ccaggcttct tgtatgtctc aaaatgccag ccttctgaaa      240

gtatacagca aagaggacca ggatttactt aaactggtga agtcatatca ttggatggga      300

ctagtacaca ttccaacaaa tggatcttgg cagtgggaag atggctccat tctctcaccc      360

aacctactaa caataattga aatgcagaag ggagactgtg cactctatgc ctcgagcttt      420

aaaggctata tagaaaactg ttcaactcca aatacgtaca tctgtatgca aaggactgtg      480

gctagcaact ggtcacaccc ccagtttgag aaaggtgggg gagggtcagg tggtgggggg      540

tcaaattggt ctcatcccca gttcgagaag ggcggaggag gttcaggtgg gggtgggagc      600

aactggagcc accctcaatt tgaaaaagga ggtggaggga gtggaggtgg tggatctgaa      660

tctaaatatg gccccccttg cccaccatgt cccttctggg ttttggtagt tgtcggcggg      720

gtgcttgctt gctattcatt gttggttacg gtggctttta taattttctg ggtatacttc      780

ctgggacgac ttgttcctcg ggggcggggt gctgcagaag ctgcaacgcg aaaacaaaga      840

attaccgaga ctgagagtcc gtatcaagaa ctgcagggcc aaagaagcga cgtttactcc      900

gatctcaata cccaacggcc ttactacaaa                                       930


<210>  27
<211>  371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CCR without signal peptide

<400>  27

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Gly Phe Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Thr Ala Tyr Asn Gly Asn Thr Asn Tyr Ala Gln Lys Leu 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Tyr Phe Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
    130                 135                 140                 


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser 
145                 150                 155                 160 


Val Ser Ser Tyr Leu Val Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
                165                 170                 175     


Arg Leu Leu Ile Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala 
            180                 185                 190         


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
        195                 200                 205             


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser 
    210                 215                 220                 


Asn Trp Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ala 
225                 230                 235                 240 


Ser Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala 
                245                 250                 255     


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
            260                 265                 270         


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
        275                 280                 285             


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
    290                 295                 300                 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
305                 310                 315                 320 


Asn His Arg Asn Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu 
                325                 330                 335     


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
            340                 345                 350         


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
        355                 360                 365             


Cys Glu Leu 
    370     


<210>  28
<211>  1113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CCR without signal peptide

<400>  28
caggttcagc tggtgcagtc tggagctgag gtgaagaagc ctggggcctc agtgaaggtc       60

tcctgcaagg cttctggtta cacctttacc gactatggtt tcagctgggt gcgacaggcc      120

cctggacaag ggcttgagtg gatgggatgg atcaccgctt acaatggtaa cacaaactat      180

gcacagaagc tccagggcag agtcaccatg accacagaca catccacgag cacagtctac      240

atggagctga ggagcctgag atctgacgac acggccgtgt attactgtgc gagagactac      300

ttctacggta tggacgtctg gggccaaggg accacggtca ccgtctcctc aggtggaggc      360

ggttcaggcg gaggtggcag cggcggtggc gggtcggaaa ttgtgttgac acagtctcca      420

gccaccctgt ctttgtctcc aggggaaaga gccaccctct cctgcagggc cagtcagagt      480

gttagcagct acttagtctg gtaccaacag aaacctggcc aggctcccag gctcctcatc      540

tatgatgcat ccaacagggc cactggcatc ccagccaggt tcagtggcag tgggtctggg      600

acagacttca ctctcaccat cagcagccta gagcctgaag attttgcagt ttattactgt      660

cagcagcgta gcaactggcc tcggacgttc ggccaaggga ccaaggtgga aatcaaagct      720

agcttcgtgc cggtcttcct gccagcgaag cccaccacga cgccagcgcc gcgaccacca      780

acaccggcgc ccaccatcgc gtcgcagccc ctgtccctgc gcccagaggc gtgccggcca      840

gcggcggggg gcgcagtgca cacgaggggg ctggacttcg cctgtgatat ctacatctgg      900

gcgcccttgg ccgggacttg tggggtcctt ctcctgtcac tggttatcac cctttactgc      960

aaccacagga accgtttctc tgttgttaaa cggggcagaa agaagctcct gtatatattc     1020

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga     1080

tttccagaag aagaagaagg aggatgtgaa ctg                                  1113


<210>  29
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CCR with signal peptide

<400>  29

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gly Ala His Ala Gln Val Gln Leu Val Gln 
            20                  25                  30          


Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys 
        35                  40                  45              


Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr Gly Phe Ser Trp Val Arg 
    50                  55                  60                  


Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Trp Ile Thr Ala Tyr 
65                  70                  75                  80  


Asn Gly Asn Thr Asn Tyr Ala Gln Lys Leu Gln Gly Arg Val Thr Met 
                85                  90                  95      


Thr Thr Asp Thr Ser Thr Ser Thr Val Tyr Met Glu Leu Arg Ser Leu 
            100                 105                 110         


Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Phe Tyr 
        115                 120                 125             


Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile 
145                 150                 155                 160 


Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg 
                165                 170                 175     


Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Val 
            180                 185                 190         


Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Asp 
        195                 200                 205             


Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp 
225                 230                 235                 240 


Phe Ala Val Tyr Tyr Cys Gln Gln Arg Ser Asn Trp Pro Arg Thr Phe 
                245                 250                 255     


Gly Gln Gly Thr Lys Val Glu Ile Lys Ala Ser Phe Val Pro Val Phe 
            260                 265                 270         


Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
        275                 280                 285             


Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
    290                 295                 300                 


Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
305                 310                 315                 320 


Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu 
                325                 330                 335     


Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Phe 
            340                 345                 350         


Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
        355                 360                 365             


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
    370                 375                 380                 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
385                 390                 395         


<210>  30
<211>  1191
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CCR with signal peptide

<400>  30
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccaggcg cgcatgccca ggttcagctg gtgcagtctg gagctgaggt gaagaagcct      120

ggggcctcag tgaaggtctc ctgcaaggct tctggttaca cctttaccga ctatggtttc      180

agctgggtgc gacaggcccc tggacaaggg cttgagtgga tgggatggat caccgcttac      240

aatggtaaca caaactatgc acagaagctc cagggcagag tcaccatgac cacagacaca      300

tccacgagca cagtctacat ggagctgagg agcctgagat ctgacgacac ggccgtgtat      360

tactgtgcga gagactactt ctacggtatg gacgtctggg gccaagggac cacggtcacc      420

gtctcctcag gtggaggcgg ttcaggcgga ggtggcagcg gcggtggcgg gtcggaaatt      480

gtgttgacac agtctccagc caccctgtct ttgtctccag gggaaagagc caccctctcc      540

tgcagggcca gtcagagtgt tagcagctac ttagtctggt accaacagaa acctggccag      600

gctcccaggc tcctcatcta tgatgcatcc aacagggcca ctggcatccc agccaggttc      660

agtggcagtg ggtctgggac agacttcact ctcaccatca gcagcctaga gcctgaagat      720

tttgcagttt attactgtca gcagcgtagc aactggcctc ggacgttcgg ccaagggacc      780

aaggtggaaa tcaaagctag cttcgtgccg gtcttcctgc cagcgaagcc caccacgacg      840

ccagcgccgc gaccaccaac accggcgccc accatcgcgt cgcagcccct gtccctgcgc      900

ccagaggcgt gccggccagc ggcggggggc gcagtgcaca cgagggggct ggacttcgcc      960

tgtgatatct acatctgggc gcccttggcc gggacttgtg gggtccttct cctgtcactg     1020

gttatcaccc tttactgcaa ccacaggaac cgtttctctg ttgttaaacg gggcagaaag     1080

aagctcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa     1140

gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact g              1191


<210>  31
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST2 tag

<400>  31

Asn Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Gly Ser Asn Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Gly 
            20                  25                  30          


Ser Gly Gly Gly Gly Ser Asn Trp Ser His Pro Gln Phe Glu Lys Gly 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    50                  55          


<210>  32
<211>  171
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ST2 tag

<400>  32
aactggtcac acccccagtt tgagaaaggt gggggagggt caggtggtgg ggggtcaaat       60

tggtctcatc cccagttcga gaagggcgga ggaggttcag gtgggggtgg gagcaactgg      120

agccaccctc aatttgaaaa aggaggtgga gggagtggag gtggtggatc t               171


 ctacggtatg gacgtctggg gccaagggac cacggtcacc      420

gtctcctcag gtggaggcgg ttcaggcgga ggtggcagcg gcggtggcgg gtcggaaatt      480

gtgttgacac agtctccagc caccctgtct ttgtctccag gggaaagagc caccctctcc      540

tgcagggcca gtcagagtgt tagcagctac ttagtctggt accaacagaa acctggccag      600

gctcccaggc tcctcatcta tgatgcatcc aacagggcca ctggcatccc agccaggttc      660

agtggcagtg ggtctgggac agacttcact ctcaccatca gcagcctaga gcctgaagat      720

tttgcagttt attactgtca gcagcgtagc aactggcctc ggacgttcgg ccaagggacc      780

aaggtggaaa tcaaagctag cttcgtgccg gtcttcctgc cagcgaagcc caccacgacg      840

ccagcgccgc gaccaccaac accggcgccc accatcgcgt cgcagcccct gtccctgcgc      900

ccagaggcgt gccggccagc ggcggggggc gcagtgcaca cgagggggct ggacttcgcc      960

tgtgatatct acatctgggc gcccttggcc gggacttgtg gggtccttct cctgtcactg     1020

gttatcaccc tttactgcaa ccacaggaac cgtttctctg ttgttaaacg gggcagaaag     1080

aagctcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa     1140

gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact g              1191


<210>  31
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ST2 tag

<400>  31

Asn