                         SEQUENCE LISTING

<110>  University of Stellenbosch
 
<120>  IFIT POLYPEPTIDES AND USES FOR TREATING TUBERCULOSIS INFECTION

<130>  PA172249/P

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  478
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ser Thr Asn Gly Asp Asp His Gln Val Lys Asp Ser Leu Glu Gln 
1               5                   10                  15      


Leu Arg Cys His Phe Thr Trp Glu Leu Ser Ile Asp Asp Asp Glu Met 
            20                  25                  30          


Pro Asp Leu Glu Asn Arg Val Leu Asp Gln Ile Glu Phe Leu Asp Thr 
        35                  40                  45              


Lys Tyr Ser Val Gly Ile His Asn Leu Leu Ala Tyr Val Lys His Leu 
    50                  55                  60                  


Lys Gly Gln Asn Glu Glu Ala Leu Lys Ser Leu Lys Glu Ala Glu Asn 
65                  70                  75                  80  


Leu Met Gln Glu Glu His Asp Asn Gln Ala Asn Val Arg Ser Leu Val 
                85                  90                  95      


Thr Trp Gly Asn Phe Ala Trp Met Tyr Tyr His Met Gly Arg Leu Ala 
            100                 105                 110         


Glu Ala Gln Thr Tyr Leu Asp Lys Val Glu Asn Ile Cys Lys Lys Leu 
        115                 120                 125             


Ser Asn Pro Phe Arg Tyr Arg Met Glu Cys Pro Glu Ile Asp Cys Glu 
    130                 135                 140                 


Glu Gly Trp Ala Leu Leu Lys Cys Gly Gly Lys Asn Tyr Glu Arg Ala 
145                 150                 155                 160 


Lys Ala Cys Phe Glu Lys Val Leu Glu Val Asp Pro Glu Asn Pro Glu 
                165                 170                 175     


Ser Ser Ala Gly Tyr Ala Ile Ser Ala Tyr Arg Leu Asp Gly Phe Lys 
            180                 185                 190         


Leu Ala Thr Lys Asn His Lys Pro Phe Ser Leu Leu Pro Leu Arg Gln 
        195                 200                 205             


Ala Val Arg Leu Asn Pro Asp Asn Gly Tyr Ile Lys Val Leu Leu Ala 
    210                 215                 220                 


Leu Lys Leu Gln Asp Glu Gly Gln Glu Ala Glu Gly Glu Lys Tyr Ile 
225                 230                 235                 240 


Glu Glu Ala Leu Ala Asn Met Ser Ser Gln Thr Tyr Val Phe Arg Tyr 
                245                 250                 255     


Ala Ala Lys Phe Tyr Arg Arg Lys Gly Ser Val Asp Lys Ala Leu Glu 
            260                 265                 270         


Leu Leu Lys Lys Ala Leu Gln Glu Thr Pro Thr Ser Val Leu Leu His 
        275                 280                 285             


His Gln Ile Gly Leu Cys Tyr Lys Ala Gln Met Ile Gln Ile Lys Glu 
    290                 295                 300                 


Ala Thr Lys Gly Gln Pro Arg Gly Gln Asn Arg Glu Lys Leu Asp Lys 
305                 310                 315                 320 


Met Ile Arg Ser Ala Ile Phe His Phe Glu Ser Ala Val Glu Lys Lys 
                325                 330                 335     


Pro Thr Phe Glu Val Ala His Leu Asp Leu Ala Arg Met Tyr Ile Glu 
            340                 345                 350         


Ala Gly Asn His Arg Lys Ala Glu Glu Asn Phe Gln Lys Leu Leu Cys 
        355                 360                 365             


Met Lys Pro Val Val Glu Glu Thr Met Gln Asp Ile His Phe His Tyr 
    370                 375                 380                 


Gly Arg Phe Gln Glu Phe Gln Lys Lys Ser Asp Val Asn Ala Ile Ile 
385                 390                 395                 400 


His Tyr Leu Lys Ala Ile Lys Ile Glu Gln Ala Ser Leu Thr Arg Asp 
                405                 410                 415     


Lys Ser Ile Asn Ser Leu Lys Lys Leu Val Leu Arg Lys Leu Arg Arg 
            420                 425                 430         


Lys Ala Leu Asp Leu Glu Ser Leu Ser Leu Leu Gly Phe Val Tyr Lys 
        435                 440                 445             


Leu Glu Gly Asn Met Asn Glu Ala Leu Glu Tyr Tyr Glu Arg Ala Leu 
    450                 455                 460                 


Arg Leu Ala Ala Asp Phe Glu Asn Ser Val Arg Gln Gly Pro 
465                 470                 475             


<210>  2
<211>  472
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ser Glu Asn Asn Lys Asn Ser Leu Glu Ser Ser Leu Arg Gln Leu 
1               5                   10                  15      


Lys Cys His Phe Thr Trp Asn Leu Met Glu Gly Glu Asn Ser Leu Asp 
            20                  25                  30          


Asp Phe Glu Asp Lys Val Phe Tyr Arg Thr Glu Phe Gln Asn Arg Glu 
        35                  40                  45              


Phe Lys Ala Thr Met Cys Asn Leu Leu Ala Tyr Leu Lys His Leu Lys 
    50                  55                  60                  


Gly Gln Asn Glu Ala Ala Leu Glu Cys Leu Arg Lys Ala Glu Glu Leu 
65                  70                  75                  80  


Ile Gln Gln Glu His Ala Asp Gln Ala Glu Ile Arg Ser Leu Val Thr 
                85                  90                  95      


Trp Gly Asn Tyr Ala Trp Val Tyr Tyr His Met Gly Arg Leu Ser Asp 
            100                 105                 110         


Val Gln Ile Tyr Val Asp Lys Val Lys His Val Cys Glu Lys Phe Ser 
        115                 120                 125             


Ser Pro Tyr Arg Ile Glu Ser Pro Glu Leu Asp Cys Glu Glu Gly Trp 
    130                 135                 140                 


Thr Arg Leu Lys Cys Gly Gly Asn Gln Asn Glu Arg Ala Lys Val Cys 
145                 150                 155                 160 


Phe Glu Lys Ala Leu Glu Lys Lys Pro Lys Asn Pro Glu Phe Thr Ser 
                165                 170                 175     


Gly Leu Ala Ile Ala Ser Tyr Arg Leu Asp Asn Trp Pro Pro Ser Gln 
            180                 185                 190         


Asn Ala Ile Asp Pro Leu Arg Gln Ala Ile Arg Leu Asn Pro Asp Asn 
        195                 200                 205             


Gln Tyr Leu Lys Val Leu Leu Ala Leu Lys Leu His Lys Met Arg Glu 
    210                 215                 220                 


Glu Gly Glu Glu Glu Gly Glu Gly Glu Lys Leu Val Glu Glu Ala Leu 
225                 230                 235                 240 


Glu Lys Ala Pro Gly Val Thr Asp Val Leu Arg Ser Ala Ala Lys Phe 
                245                 250                 255     


Tyr Arg Arg Lys Asp Glu Pro Asp Lys Ala Ile Glu Leu Leu Lys Lys 
            260                 265                 270         


Ala Leu Glu Tyr Ile Pro Asn Asn Ala Tyr Leu His Cys Gln Ile Gly 
        275                 280                 285             


Cys Cys Tyr Arg Ala Lys Val Phe Gln Val Met Asn Leu Arg Glu Asn 
    290                 295                 300                 


Gly Met Tyr Gly Lys Arg Lys Leu Leu Glu Leu Ile Gly His Ala Val 
305                 310                 315                 320 


Ala His Leu Lys Lys Ala Asp Glu Ala Asn Asp Asn Leu Phe Arg Val 
                325                 330                 335     


Cys Ser Ile Leu Ala Ser Leu His Ala Leu Ala Asp Gln Tyr Glu Asp 
            340                 345                 350         


Ala Glu Tyr Tyr Phe Gln Lys Glu Phe Ser Lys Glu Leu Thr Pro Val 
        355                 360                 365             


Ala Lys Gln Leu Leu His Leu Arg Tyr Gly Asn Phe Gln Leu Tyr Gln 
    370                 375                 380                 


Met Lys Cys Glu Asp Lys Ala Ile His His Phe Ile Glu Gly Val Lys 
385                 390                 395                 400 


Ile Asn Gln Lys Ser Arg Glu Lys Glu Lys Met Lys Asp Lys Leu Gln 
                405                 410                 415     


Lys Ile Ala Lys Met Arg Leu Ser Lys Asn Gly Ala Asp Ser Glu Ala 
            420                 425                 430         


Leu His Val Leu Ala Phe Leu Gln Glu Leu Asn Glu Lys Met Gln Gln 
        435                 440                 445             


Ala Asp Glu Asp Ser Glu Arg Gly Leu Glu Ser Gly Ser Leu Ile Pro 
    450                 455                 460                 


Ser Ala Ser Ser Trp Asn Gly Glu 
465                 470         


<210>  3
<211>  490
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Glu Val Thr Lys Asn Ser Leu Glu Lys Ile Leu Pro Gln Leu 
1               5                   10                  15      


Lys Cys His Phe Thr Trp Asn Leu Phe Lys Glu Asp Ser Val Ser Arg 
            20                  25                  30          


Asp Leu Glu Asp Arg Val Cys Asn Gln Ile Glu Phe Leu Asn Thr Glu 
        35                  40                  45              


Phe Lys Ala Thr Met Tyr Asn Leu Leu Ala Tyr Ile Lys His Leu Asp 
    50                  55                  60                  


Gly Asn Asn Glu Ala Ala Leu Glu Cys Leu Arg Gln Ala Glu Glu Leu 
65                  70                  75                  80  


Ile Gln Gln Glu His Ala Asp Gln Ala Glu Ile Arg Ser Leu Val Thr 
                85                  90                  95      


Trp Gly Asn Tyr Ala Trp Val Tyr Tyr His Leu Gly Arg Leu Ser Asp 
            100                 105                 110         


Ala Gln Ile Tyr Val Asp Lys Val Lys Gln Thr Cys Lys Lys Phe Ser 
        115                 120                 125             


Asn Pro Tyr Ser Ile Glu Tyr Ser Glu Leu Asp Cys Glu Glu Gly Trp 
    130                 135                 140                 


Thr Gln Leu Lys Cys Gly Arg Asn Glu Arg Ala Lys Val Cys Phe Glu 
145                 150                 155                 160 


Lys Ala Leu Glu Glu Lys Pro Asn Asn Pro Glu Phe Ser Ser Gly Leu 
                165                 170                 175     


Ala Ile Ala Met Tyr His Leu Asp Asn His Pro Glu Lys Gln Phe Ser 
            180                 185                 190         


Thr Asp Val Leu Lys Gln Ala Ile Glu Leu Ser Pro Asp Asn Gln Tyr 
        195                 200                 205             


Val Lys Val Leu Leu Gly Leu Lys Leu Gln Lys Met Asn Lys Glu Ala 
    210                 215                 220                 


Glu Gly Glu Gln Phe Val Glu Glu Ala Leu Glu Lys Ser Pro Cys Gln 
225                 230                 235                 240 


Thr Asp Val Leu Arg Ser Ala Ala Lys Phe Tyr Arg Arg Lys Gly Asp 
                245                 250                 255     


Leu Asp Lys Ala Ile Glu Leu Phe Gln Arg Val Leu Glu Ser Thr Pro 
            260                 265                 270         


Asn Asn Gly Tyr Leu Tyr His Gln Ile Gly Cys Cys Tyr Lys Ala Lys 
        275                 280                 285             


Val Arg Gln Met Gln Asn Thr Gly Glu Ser Glu Ala Ser Gly Asn Lys 
    290                 295                 300                 


Glu Met Ile Glu Ala Leu Lys Gln Tyr Ala Met Asp Tyr Ser Asn Lys 
305                 310                 315                 320 


Ala Leu Glu Lys Gly Leu Asn Pro Leu Asn Ala Tyr Ser Asp Leu Ala 
                325                 330                 335     


Glu Phe Leu Glu Thr Glu Cys Tyr Gln Thr Pro Phe Asn Lys Glu Val 
            340                 345                 350         


Pro Asp Ala Glu Lys Gln Gln Ser His Gln Arg Tyr Cys Asn Leu Gln 
        355                 360                 365             


Lys Tyr Asn Gly Lys Ser Glu Asp Thr Ala Val Gln His Gly Leu Glu 
    370                 375                 380                 


Gly Leu Ser Ile Ser Lys Lys Ser Thr Asp Lys Glu Glu Ile Lys Asp 
385                 390                 395                 400 


Gln Pro Gln Asn Val Ser Glu Asn Leu Leu Pro Gln Asn Ala Pro Asn 
                405                 410                 415     


Tyr Trp Tyr Leu Gln Gly Leu Ile His Lys Gln Asn Gly Asp Leu Leu 
            420                 425                 430         


Gln Ala Ala Lys Cys Tyr Glu Lys Glu Leu Gly Arg Leu Leu Arg Asp 
        435                 440                 445             


Ala Pro Ser Gly Ile Gly Ser Ile Phe Leu Ser Ala Ser Glu Leu Glu 
    450                 455                 460                 


Asp Gly Ser Glu Glu Met Gly Gln Gly Ala Val Ser Ser Ser Pro Arg 
465                 470                 475                 480 


Glu Leu Leu Ser Asn Ser Glu Gln Leu Asn 
                485                 490 


<210>  4
<211>  1437
<212>  DNA
<213>  Homo sapiens

<400>  4
atgagtacaa atggtgatga tcatcaggtc aaggatagtc tggagcaatt gagatgtcac       60

tttacatggg agttatccat tgatgacgat gaaatgcctg atttagaaaa cagagtcttg      120

gatcagattg aattcctaga caccaaatac agtgtgggaa tacacaacct actagcctat      180

gtgaaacacc tgaaaggcca gaatgaggaa gccctgaaga gcttaaaaga agctgaaaac      240

ttaatgcagg aagaacatga caaccaagca aatgtgagga gtctggtgac ctggggcaac      300

tttgcctgga tgtattacca catgggcaga ctggcagaag cccagactta cctggacaag      360

gtggagaaca tttgcaagaa gctttcaaat cccttccgct atagaatgga gtgtccagaa      420

atagactgtg aggaaggatg ggccttgctg aagtgtggag gaaaaaatta tgaacgggcc      480

aaggcctgct ttgaaaaggt gcttgaagtg gaccctgaaa accctgaatc cagcgctggg      540

tatgcgatct ctgcctatcg cctggatggc tttaaattag ccacaaaaaa tcacaagcca      600

ttttctttgc ttcccctaag gcaggctgtc cgcttaaatc cagacaatgg atatattaag      660

gttctccttg ccctgaagct tcaggatgaa ggacaggaag ctgaaggaga aaagtacatt      720

gaagaagctc tagccaacat gtcctcacag acctatgtct ttcgatatgc agccaagttt      780

taccgaagaa aaggctctgt ggataaagct cttgagttat taaaaaaggc cttgcaggaa      840

acacccactt ctgtcttact gcatcaccag atagggcttt gctacaaggc acaaatgatc      900

caaatcaagg aggctacaaa agggcagcct agagggcaga acagagaaaa gctagacaaa      960

atgataagat cagccatatt tcattttgaa tctgcagtgg aaaaaaagcc cacatttgag     1020

gtggctcatc tagacctggc aagaatgtat atagaagcag gcaatcacag aaaagctgaa     1080

gagaattttc aaaaattgtt atgcatgaaa ccagtggtag aagaaacaat gcaagacata     1140

catttccact atggtcggtt tcaggaattt caaaagaaat ctgacgtcaa tgcaattatc     1200

cattatttaa aagctataaa aatagaacag gcatcattaa caagggataa aagtatcaat     1260

tctttgaaga aattggtttt aaggaaactt cggagaaagg cattagatct ggaaagcttg     1320

agcctccttg ggttcgtcta caaattggaa ggaaatatga atgaagccct ggagtactat     1380

gagcgggccc tgagactggc tgctgacttt gagaactctg tgagacaagg tccttag        1437


<210>  5
<211>  1419
<212>  DNA
<213>  Homo sapiens

<400>  5
atgagtgaga acaataagaa ttccttggag agcagcctac ggcaactaaa atgccatttc       60

acctggaact tgatggaggg agaaaactcc ttggatgatt ttgaagacaa agtattttac      120

cggactgagt ttcagaatcg tgaattcaaa gccacaatgt gcaacctact ggcctatcta      180

aagcacctca aagggcaaaa cgaggcagcc ctggaatgct tacgtaaagc tgaagagtta      240

atccagcaag agcatgctga ccaggcagaa atcagaagtc tggtcacctg gggaaactat      300

gcctgggtct actatcacat gggccgactc tcagacgttc agatttatgt agacaaggtg      360

aaacatgtct gtgagaagtt ttccagtccc tatagaattg agagtccaga gcttgactgt      420

gaggaagggt ggacacggtt aaagtgtgga ggaaaccaaa atgaaagagc gaaggtgtgc      480

tttgagaagg ctctggaaaa gaagccaaag aacccagaat tcacctctgg actggcaata      540

gcaagctacc gtctggacaa ctggccacca tctcagaacg ccattgaccc tctgaggcaa      600

gccattcggc tgaatcctga caaccagtac cttaaagtcc tcctggctct gaagcttcat      660

aagatgcgtg aagaaggtga agaggaaggt gaaggagaga agttagttga agaagccttg      720

gagaaagccc caggtgtaac agatgttctt cgcagtgcag ccaagtttta tcgaagaaaa      780

gatgagccag acaaagcgat tgaactgctt aaaaaggctt tagaatacat accaaacaat      840

gcctacctgc attgccaaat tgggtgctgc tatagggcaa aagtcttcca agtaatgaat      900

ctaagagaga atggaatgta tgggaaaaga aagttactgg aactaatagg acacgctgtg      960

gctcatctga agaaagctga tgaggccaat gataatctct tccgtgtctg ttccattctt     1020

gccagcctcc atgctctagc agatcagtat gaagacgcag agtattactt ccaaaaggaa     1080

ttcagtaaag agcttactcc tgtagcgaaa caactgctcc atctgcggta tggcaacttt     1140

cagctgtacc aaatgaagtg tgaagacaag gccatccacc actttataga gggtgtaaaa     1200

ataaaccaga aatcaaggga gaaagaaaag atgaaagaca aactgcaaaa aattgccaaa     1260

atgcgacttt ctaaaaatgg agcagattct gaggctttgc atgtcttggc attccttcag     1320

gagctgaatg aaaaaatgca acaagcagat gaagactctg agaggggttt ggagtctgga     1380

agcctcatcc cttcagcatc aagctggaat ggggaatga                            1419


<210>  6
<211>  1473
<212>  DNA
<213>  Homo sapiens

<400>  6
atgagtgagg tcaccaagaa ttccctggag aaaatccttc cacagctgaa atgccatttc       60

acctggaact tattcaagga agacagtgtc tcaagggatc tagaagatag agtgtgtaac      120

cagattgaat ttttaaacac tgagttcaaa gctacaatgt acaacttgtt ggcctacata      180

aaacacctag atggtaacaa cgaggcagcc ctggaatgct tacggcaagc tgaagagtta      240

atccagcaag aacatgctga ccaagcagaa atcagaagtc tagtcacttg gggaaactac      300

gcctgggtct actatcactt gggcagactc tcagatgctc agatttatgt agataaggtg      360

aaacaaacct gcaagaaatt ttcaaatcca tacagtattg agtattctga acttgactgt      420

gaggaagggt ggacacaact gaagtgtgga agaaatgaaa gggcgaaggt gtgttttgag      480

aaggctctgg aagaaaagcc caacaaccca gaattctcct ctggactggc aattgcgatg      540

taccatctgg ataatcaccc agagaaacag ttctctactg atgttttgaa gcaggccatt      600

gagctgagtc ctgataacca atacgtcaag gttctcttgg gcctgaaact gcagaagatg      660

aataaagaag ctgaaggaga gcagtttgtt gaagaagcct tggaaaagtc tccttgccaa      720

acagatgtcc tccgcagtgc agccaaattt tacagaagaa aaggtgacct agacaaagct      780

attgaactgt ttcaacgggt gttggaatcc acaccaaaca atggctacct ctatcaccag      840

attgggtgct gctacaaggc aaaagtaaga caaatgcaga atacaggaga atctgaagct      900

agtggaaata aagagatgat tgaagcacta aagcaatatg ctatggacta ttcgaataaa      960

gctcttgaga agggactgaa tcctctgaat gcatactccg atctcgctga gttcctggag     1020

acggaatgtt atcagacacc attcaataag gaagtccctg atgctgaaaa gcaacaatcc     1080

catcagcgct actgcaacct tcagaaatat aatgggaagt ctgaagacac tgctgtgcaa     1140

catggtttag agggtttgtc cataagcaaa aaatcaactg acaaggaaga gatcaaagac     1200

caaccacaga atgtatctga aaatctgctt ccacaaaatg caccaaatta ttggtatctt     1260

caaggattaa ttcataagca gaatggagat ctgctgcaag cagccaaatg ttatgagaag     1320

gaactgggcc gcctgctaag ggatgcccct tcaggcatag gcagtatttt cctgtcagca     1380

tctgagcttg aggatggtag tgaggaaatg ggccagggcg cagtcagctc cagtcccaga     1440

gagctcctct ctaactcaga gcaactgaac tga                                  1473


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid target sequence 1 of IFIT1 (Catalogue Number 
       S100445879)

<400>  7
caggctgtcc gcttaaatcc a                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid target sequence 2 of IFIT1 (Catalogue Number 
       S103224284)

<400>  8
tacatgggag ttatccattg a                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid target sequence 1 of IFIT2 (Catalogue Number 
       S104145372)

<400>  9
aaagaaagtt actggaacta a                                                 21


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid target sequence 2 of IFIT2 (Catalogue Number 
       S104259010)

<400>  10
cccatagagg ttagtcctgc a                                                 21


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid target sequence 1 of IFIT3 (Catalogue Number 
       S103152737)

<400>  11
atgctatgga ctattcgaat a                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid target sequence 2 of IFIT3 (Catalogue Number 
       S104197788)

<400>  12
agagatgatt gaagcactaa a                                                 21


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of Hs_GAPDH Forward primer

<400>  13
tgcaccacca actgcttagc                                                   20


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of Hs_GAPDH Reverse primer

<400>  14
ggcatggact gtggtcatga g                                                 21


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of Hs_UBC Forward primer

<400>  15
cggtgaacgc cgatgattat                                                   20


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of Hs_UBC Reverse primer

<400>  16
atctgcattg tcaagtgacg a                                                 21


