                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  ENGINEERED IMMUNE CELL

<130>  P118638PCT

<150>  GB 1914611.7
<151>  2019-10-09

<160>  180   

<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif to direct proteins to the Golgi apparatus

<400>  1

Ser Glu Lys Asp Glu Leu 
1               5       


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golgi retention signal sequence

<400>  2

Lys Asp Glu Leu 
1               


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golgi retention signal sequence


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Lys Lys Xaa Xaa 
1               


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golgi retention signal sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Lys Xaa Lys Xaa Xaa 
1               5   


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golgi retention signal sequence

<400>  5

Lys Tyr Lys Ser Arg Arg Ser Phe Ile Asp Glu Lys Lys Met Pro 
1               5                   10                  15  


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golgi retention signal sequence

<400>  6

Met His Arg Arg Arg Ser Arg Ser Cys Arg 
1               5                   10  


<210>  7
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golgi retention signal sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Asp or Glu

<400>  7

Lys Xaa Xaa 
1           


<210>  8
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Golgi retention signal sequence

<400>  8

Tyr Gln Arg Leu 
1               


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  9

Gly Gly Gly Gly Ser 
1               5   


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  10

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  11
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  11

Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10              


<210>  12
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ectodomain from CD79a

<400>  12

Leu Trp Met His Lys Val Pro Ala Ser Leu Met Val Ser Leu Gly Glu 
1               5                   10                  15      


Asp Ala His Phe Gln Cys Pro His Asn Ser Ser Asn Asn Ala Asn Val 
            20                  25                  30          


Thr Trp Trp Arg Val Leu His Gly Asn Tyr Thr Trp Pro Pro Glu Phe 
        35                  40                  45              


Leu Gly Pro Gly Glu Asp Pro Asn Gly Thr Leu Ile Ile Gln Asn Val 
    50                  55                  60                  


Asn Lys Ser His Gly Gly Ile Tyr Val Cys Arg Val Gln Glu Gly Asn 
65                  70                  75                  80  


Glu Ser Tyr Gln Gln Ser Cys Gly Thr Tyr Leu Arg Val Arg Gln Pro 
                85                  90                  95      


Pro Pro Arg Pro Phe Leu Asp Met Gly Glu Gly Thr Lys Asn Arg 
            100                 105                 110     


<210>  13
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ectodomain from CD79b

<400>  13

Ala Arg Ser Glu Asp Arg Tyr Arg Asn Pro Lys Gly Ser Ala Cys Ser 
1               5                   10                  15      


Arg Ile Trp Gln Ser Pro Arg Phe Ile Ala Arg Lys Arg Gly Phe Thr 
            20                  25                  30          


Val Lys Met His Cys Tyr Met Asn Ser Ala Ser Gly Asn Val Ser Trp 
        35                  40                  45              


Leu Trp Lys Gln Glu Met Asp Glu Asn Pro Gln Gln Leu Lys Leu Glu 
    50                  55                  60                  


Lys Gly Arg Met Glu Glu Ser Gln Asn Glu Ser Leu Ala Thr Leu Thr 
65                  70                  75                  80  


Ile Gln Gly Ile Arg Phe Glu Asp Asn Gly Ile Tyr Phe Cys Gln Gln 
                85                  90                  95      


Lys Cys Asn Asn Thr Ser Glu Val Tyr Gln Gly Cys Gly Thr Glu Leu 
            100                 105                 110         


Arg Val Met Gly Phe Ser Thr Leu Ala Gln Leu Lys Gln Arg Asn Thr 
        115                 120                 125             


Leu Lys Asp 
    130     


<210>  14
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kappa chain constant region

<400>  14

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  15
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CH1 region

<400>  15

Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
1               5                   10                  15      


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
            20                  25                  30          


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
        35                  40                  45              


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
    50                  55                  60                  


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
65                  70                  75                  80  


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
                85                  90                  95      


Val 
    


<210>  16
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane domain, Tyrp-TM

<400>  16

Ile Ile Ala Ile Ala Val Val Gly Ala Leu Leu Leu Val Ala Leu Ile 
1               5                   10                  15      


Phe Gly Thr Ala Ser Tyr Leu Ile 
            20                  


<210>  17
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane domain, dCD20_TM

<400>  17

Ile Met Asn Gly Leu Phe His Ile Ala Leu Gly Gly Leu Leu Met Ile 
1               5                   10                  15      


Pro Ala Gly Ile Tyr Ala 
            20          


<210>  18
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer domain, CD8STK

<400>  18

Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile 
1               5                   10                  15      


Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala 
            20                  25                  30          


Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45          


<210>  19
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer domain, dCD20_N-terminal

<400>  19

Thr Thr Pro Arg Asn Ser Val Asn Gly Thr Phe Pro Ala Glu Pro Met 
1               5                   10                  15      


Lys Gly Pro Ile Ala Met Gln Ser Gly Pro Lys Pro Leu Phe Arg Arg 
            20                  25                  30          


Met Ser Ser Leu Val Gly Pro Thr Gln Ser Phe Phe Met Arg Glu Ser 
        35                  40                  45              


Lys Thr Leu Gly Ala Val Gln 
    50                  55  


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer domain, dCD20_Short_Loop

<400>  20

Pro Ile Cys Val Thr Val 
1               5       


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ALFA_tag peptide

<400>  21

Pro Ser Arg Leu Glu Glu Glu Leu Arg Arg Arg Leu Thr Glu Pro 
1               5                   10                  15  


<210>  22
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-ALFA_Tag (nanobody NbALFA) peptide

<400>  22

Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Val Thr Ile Ser Ala Leu 
            20                  25                  30          


Asn Ala Met Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Glu Arg Arg 
        35                  40                  45              


Val Met Val Ala Ala Val Ser Glu Arg Gly Asn Ala Met Tyr Arg Glu 
    50                  55                  60                  


Ser Val Gln Gly Arg Phe Thr Val Thr Arg Asp Phe Thr Asn Lys Met 
65                  70                  75                  80  


Val Ser Leu Gln Met Asp Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys His Val Leu Glu Asp Arg Val Asp Ser Phe His Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120         


<210>  23
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  epitope tag system, HA-tag

<400>  23

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  24
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  epitope tag system, FLAG-tag

<400>  24

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  25
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  epitope tag system, SPOT-tag

<400>  25

Pro Asp Arg Val Arg Ala Val Ser His Trp Ser Ser 
1               5                   10          


<210>  26
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  epitope tag system, EPEA/C-tag

<400>  26

Glu Pro Glu Ala 
1               


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  epitope tag system, myc-tag

<400>  27

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  28
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  last 6 amino acids of a tail of adenoviral E19 protein

<400>  28

Asp Glu Lys Lys Met Pro 
1               5       


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal, sequence from TYRP-1

<400>  29

Asn Gln Pro Leu Leu Thr Asp 
1               5           


<210>  30
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  target binding domain, Ab-1 (aCD3e_UCHT)

<400>  30

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asn Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn Thr Leu Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
        115                 120                 125             


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
    130                 135                 140                 


Cys Ala Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val 
145                 150                 155                 160 


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Leu Ile Asn Pro 
                165                 170                 175     


Tyr Lys Gly Val Ser Thr Tyr Asn Gln Lys Phe Lys Asp Arg Phe Thr 
            180                 185                 190         


Ile Ser Val Asp Lys Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser 
        195                 200                 205             


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr 
    210                 215                 220                 


Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  31
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  target binding domain, Ab-2 (aB2M_dN6B2m)

<400>  31

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Asp Ser Arg Tyr 
            20                  25                  30          


Cys Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Asn Ser Gly Arg Asp Ile Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Phe Ser Gln Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Glu Pro Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ile Pro Leu Arg Cys Arg Asp Ile Val Ala Lys Gly Gly 
            100                 105                 110         


Asp Gly Phe Arg Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


<210>  32
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  target binding domain, Ab-3 (aPD1_clone10)

<400>  32

Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Gly Gln Asn Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Phe Gly Val Pro 
    50                  55                  60                  


Asp Arg Ile Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Phe Gln Gly 
                85                  90                  95      


Ser His Val Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
    130                 135                 140                 


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Asp 
145                 150                 155                 160 


Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 
                165                 170                 175     


Met Gly Tyr Ile Asn Tyr Ser Gly Ser Thr Ser Tyr Asn Pro Ser Leu 
            180                 185                 190         


Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln Phe Phe 
        195                 200                 205             


Leu Gln Leu Asn Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys 
    210                 215                 220                 


Ala Arg Trp Ile Gly Ser Ser Ala Trp Tyr Phe Asp Val Trp Gly Ala 
225                 230                 235                 240 


Gly Thr Thr Val Thr Val Ser Ser 
                245             


<210>  33
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric antigen receptor (CAR) sequence, aCD19CAR

<400>  33

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Lys Ala Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser 
    130                 135                 140                 


Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp 
145                 150                 155                 160 


Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp 
                165                 170                 175     


Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu 
            180                 185                 190         


Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe 
        195                 200                 205             


Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys 
    210                 215                 220                 


Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly 
225                 230                 235                 240 


Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro Thr Thr Thr Pro Ala 
                245                 250                 255     


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
            260                 265                 270         


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
        275                 280                 285             


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
    290                 295                 300                 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
305                 310                 315                 320 


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
                325                 330                 335     


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            340                 345                 350         


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        355                 360                 365             


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    370                 375                 380                 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
385                 390                 395                 400 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                405                 410                 415     


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            420                 425                 430         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        435                 440                 445             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    450                 455                 460                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
465                 470                 


<210>  34
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  marker, RQR8

<400>  34

Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Glu 
1               5                   10                  15      


Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Pro 
            20                  25                  30          


Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys 
        35                  40                  45              


Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
    50                  55                  60                  


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
65                  70                  75                  80  


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
                85                  90                  95      


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
            100                 105                 110         


Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Val 
        115                 120                 125             


Cys Lys Cys Pro Arg Pro Val Val Arg Ala 
    130                 135             


<210>  35
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide, murine Ig kappa chain V-III region

<400>  35

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  36
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  36

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Ala 
            20      


<210>  37
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  self-cleaving peptide, 2A-like sequence

<400>  37

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  38
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR sequence, aCD19CAR

<400>  38

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Lys Ala Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser 
    130                 135                 140                 


Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp 
145                 150                 155                 160 


Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp 
                165                 170                 175     


Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu 
            180                 185                 190         


Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe 
        195                 200                 205             


Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys 
    210                 215                 220                 


Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly 
225                 230                 235                 240 


Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro Thr Thr Thr Pro Ala 
                245                 250                 255     


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
            260                 265                 270         


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
        275                 280                 285             


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
    290                 295                 300                 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
305                 310                 315                 320 


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
                325                 330                 335     


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            340                 345                 350         


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        355                 360                 365             


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    370                 375                 380                 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
385                 390                 395                 400 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                405                 410                 415     


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            420                 425                 430         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        435                 440                 445             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    450                 455                 460                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
465                 470                 


<210>  39
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  target binding domain, Ab-4 (aCD52_)

<400>  39

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Ile Asp Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Asn Thr Asn Asn Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Leu Gln His Ile Ser Arg Pro Arg 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ser Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln 
        115                 120                 125             


Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln Thr Leu Ser Leu Thr 
    130                 135                 140                 


Cys Thr Val Ser Gly Phe Thr Phe Thr Asp Phe Tyr Met Asn Trp Val 
145                 150                 155                 160 


Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile Gly Phe Ile Arg Asp 
                165                 170                 175     


Lys Ala Lys Gly Tyr Thr Thr Glu Tyr Asn Pro Ser Val Lys Gly Arg 
            180                 185                 190         


Val Thr Met Leu Val Asp Thr Ser Lys Asn Gln Phe Ser Leu Arg Leu 
        195                 200                 205             


Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu 
    210                 215                 220                 


Gly His Thr Ala Ala Pro Phe Asp Tyr Trp Gly Gln Gly Ser Leu Val 
225                 230                 235                 240 


Thr Val Ser Ser 
                


<210>  40
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  target binding domain, Ab-5 (aTBR2_E11)

<400>  40

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Ile Leu Ser Ser Lys 
            20                  25                  30          


Ala Val Ala Trp Tyr Arg Gln Pro Pro Gly Gln Gln Arg Glu Gly Val 
        35                  40                  45              


Ala His Ser Ser Val Ser Gly Thr Thr Ile Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Thr 
                85                  90                  95      


Ala Pro Val Gly His Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
            100                 105                 110         


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal sequence


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  41

Asn Pro Phe Xaa Asp 
1               5   


<210>  42
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tyrosine-based sorting signal consensus motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  42

Asn Pro Xaa Tyr 
1               


<210>  43
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tyrosine-based sorting signal consensus motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is an amino acid with a bulky hydrophobic side chain

<400>  43

Tyr Xaa Xaa Xaa 
1               


<210>  44
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  44

Ile Asn Phe Asp Asn Pro Val Tyr Gln Lys Thr Thr 
1               5                   10          


<210>  45
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  45

Val Glu Ile Gly Asn Pro Thr Tyr Lys Met Tyr Glu 
1               5                   10          


<210>  46
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  46

Thr Asn Phe Thr Asn Pro Val Tyr Ala Thr Leu Tyr 
1               5                   10          


<210>  47
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  47

Gly Asn Phe Ala Asn Pro Val Tyr Glu Ser Met Tyr 
1               5                   10          


<210>  48
<211>  12
<212>  PRT
<213>  Caenorhabditis elegans

<400>  48

Thr Thr Phe Thr Asn Pro Val Tyr Glu Leu Glu Asp 
1               5                   10          


<210>  49
<211>  12
<212>  PRT
<213>  Caenorhabditis elegans

<400>  49

Leu Arg Val Asp Asn Pro Leu Tyr Asp Pro Asp Ser 
1               5                   10          


<210>  50
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  50

Ile Ile Phe Glu Asn Pro Met Tyr Ser Ala Arg Asp 
1               5                   10          


<210>  51
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  51

Thr Asn Phe Glu Asn Pro Ile Tyr Ala Gln Met Glu 
1               5                   10          


<210>  52
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  52

Asp Thr Gly Glu Asn Pro Ile Tyr Lys Ser Ala Val 
1               5                   10          


<210>  53
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  53

Thr Thr Val Val Asn Pro Lys Tyr Glu Gly Lys 
1               5                   10      


<210>  54
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  54

Trp Asp Thr Glu Asn Pro Ile Tyr Lys Gln Ala Thr 
1               5                   10          


<210>  55
<211>  11
<212>  PRT
<213>  Drosophila sp.

<400>  55

Ser Thr Phe Lys Asn Pro Met Tyr Ala Gly Lys 
1               5                   10      


<210>  56
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  56

His Gly Tyr Glu Asn Pro Thr Tyr Arg Phe Leu Glu 
1               5                   10          


<210>  57
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  57

Asn Gly Tyr Glu Asn Pro Thr Tyr Lys Phe Phe Glu 
1               5                   10          


<210>  58
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  58

Asn Gly Tyr Glu Asn Pro Thr Tyr Lys Tyr Phe Glu 
1               5                   10          


<210>  59
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  59

Tyr Ala Ser Ser Asn Pro Glu Tyr Leu Ser Ala Ser 
1               5                   10          


<210>  60
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  60

Gly Ser Val Gln Asn Pro Val Tyr His Asn Gln Pro 
1               5                   10          


<210>  61
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  61

Thr Ala Val Gly Asn Pro Glu Tyr Leu Asn Thr Val 
1               5                   10          


<210>  62
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  62

Ile Ser Leu Asp Asn Pro Asp Tyr Gln Gln Asp Phe 
1               5                   10          


<210>  63
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  63

Arg Lys Arg Ser His Ala Gly Tyr Gln Thr Ile 
1               5                   10      


<210>  64
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  64

Lys His His His Ala Gly Tyr Glu Gln Phe 
1               5                   10  


<210>  65
<211>  11
<212>  PRT
<213>  Gallus gallus

<400>  65

Lys Lys His His Asn Thr Gly Tyr Glu Gln Phe 
1               5                   10      


<210>  66
<211>  11
<212>  PRT
<213>  Gallus gallus

<400>  66

Arg Arg Lys Ser Arg Thr Gly Tyr Gln Ser Val 
1               5                   10      


<210>  67
<211>  11
<212>  PRT
<213>  Gallus gallus

<400>  67

Arg Arg Lys Ser Tyr Ala Gly Tyr Gln Thr Leu 
1               5                   10      


<210>  68
<211>  12
<212>  PRT
<213>  Drosophila sp.

<400>  68

Arg Arg Arg Ser Thr Ser Arg Gly Tyr Met Ser Phe 
1               5                   10          


<210>  69
<211>  11
<212>  PRT
<213>  Lumbricus rubellus

<400>  69

Arg Lys Arg Ser Arg Arg Gly Tyr Glu Ser Val 
1               5                   10      


<210>  70
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  70

Lys Ser Ile Arg Ser Gly Tyr Glu Val Met 
1               5                   10  


<210>  71
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  71

His Cys Gly Gly Pro Arg Pro Gly Tyr Glu Thr Leu 
1               5                   10          


<210>  72
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  72

His Cys Arg Thr Arg Arg Ala Glu Tyr Glu Thr Leu 
1               5                   10          


<210>  73
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  73

Arg Arg Arg Pro Ser Ala Tyr Gln Ala Leu 
1               5                   10  


<210>  74
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  74

Arg Arg Arg Ser Tyr Gln Asn Ile Pro 
1               5                   


<210>  75
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  75

Lys Lys His Cys Ser Tyr Gln Asp Ile Leu 
1               5                   10  


<210>  76
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  76

Arg Arg Arg Ser Ala Tyr Gln Asp Ile Arg 
1               5                   10  


<210>  77
<211>  11
<212>  PRT
<213>  Rattus norvegicus

<400>  77

Arg Lys Arg Arg Arg Ser Tyr Gln Asp Ile Met 
1               5                   10      


<210>  78
<211>  13
<212>  PRT
<213>  Rattus norvegicus

<400>  78

Lys Phe Cys Lys Ser Lys Glu Arg Asn Tyr His Thr Leu 
1               5                   10              


<210>  79
<211>  14
<212>  PRT
<213>  Drosophila sp.

<400>  79

Lys Phe Tyr Lys Ala Arg Asn Glu Arg Asn Tyr His Thr Leu 
1               5                   10                  


<210>  80
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  80

Lys Ile Arg Leu Arg Cys Gln Ser Ser Gly Tyr Gln Arg Ile 
1               5                   10                  


<210>  81
<211>  14
<212>  PRT
<213>  Mus musculus

<400>  81

Lys Ile Arg Gln Arg His Gln Ser Ser Ala Tyr Gln Arg Ile 
1               5                   10                  


<210>  82
<211>  15
<212>  PRT
<213>  Homo sapiens

<400>  82

His Phe Cys Leu Tyr Arg Lys Arg Pro Gly Tyr Asp Gln Leu Asn 
1               5                   10                  15  


<210>  83
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  83

Ser Leu Ser Arg Gly Ser Gly Tyr Lys Glu Ile 
1               5                   10      


<210>  84
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  84

Arg Arg Leu Arg Lys Gly Tyr Thr Pro Leu Met Glu Thr 
1               5                   10              


<210>  85
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  85

Arg Arg Ala Gly His Ser Ser Tyr Thr Pro Leu Pro Gly Ser 
1               5                   10                  


<210>  86
<211>  18
<212>  PRT
<213>  Dictyostelium discoideum

<400>  86

Lys Lys Leu Arg Gln Gln Lys Gln Gln Gly Tyr Gln Ala Ile Ile Asn 
1               5                   10                  15      


Asn Glu 
        


<210>  87
<211>  15
<212>  PRT
<213>  Dictyostelium discoideum

<400>  87

Arg Ser Lys Ser Asn Gln Asn Gln Ser Tyr Asn Leu Ile Gln Leu 
1               5                   10                  15  


<210>  88
<211>  17
<212>  PRT
<213>  Dictyostelium discoideum

<400>  88

Arg Lys Thr Phe Tyr Asn Asn Asn Gln Tyr Asn Gly Tyr Asn Ile Ile 
1               5                   10                  15      


Asn 
    


<210>  89
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  89

Pro Leu Ser Tyr Thr Arg Phe Ser Leu Ala 
1               5                   10  


<210>  90
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  90

Met Thr Lys Glu Tyr Gln Asp Leu Gln His Leu 
1               5                   10      


<210>  91
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  91

Ser Tyr Lys Tyr Ser Lys Val Asn Lys Glu 
1               5                   10  


<210>  92
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  92

Pro Ala Ala Tyr Arg Gly Val Gly Asp Asp 
1               5                   10  


<210>  93
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  93

Thr Gly Val Tyr Val Lys Met Pro Pro Thr 
1               5                   10  


<210>  94
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  94

Leu Ile Ser Tyr Lys Gly Leu Pro Pro Glu 
1               5                   10  


<210>  95
<211>  11
<212>  PRT
<213>  Rattus norvegicus

<400>  95

Ala Ser Asp Tyr Gln Arg Leu Asn Leu Lys Leu 
1               5                   10      


<210>  96
<211>  11
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  96

Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr 
1               5                   10      


<210>  97
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  97

Arg Met Gln Ala Gln Pro Pro Gly Tyr Arg His Val Ala Asp Gly Glu 
1               5                   10                  15      


Asp His Ala 
            


<210>  98
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dileucine-based sorting signal sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Asp or Glu

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa may be Leu or Ile

<400>  98

Xaa Xaa Xaa Xaa Leu Leu Xaa 
1               5           


<210>  99
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  99

Ser Asp Lys Gln Thr Leu Leu Pro Asn 
1               5                   


<210>  100
<211>  9
<212>  PRT
<213>  Rattus norvegicus

<400>  100

Asp Glu Arg Ala Pro Leu Ile Arg Thr 
1               5                   


<210>  101
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  101

Gln Glu Lys Asp Pro Leu Leu Lys Asn 
1               5                   


<210>  102
<211>  9
<212>  PRT
<213>  Odontophorus gujanensis

<400>  102

Thr Glu Arg Asn Pro Leu Leu Lys Ser 
1               5                   


<210>  103
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  103

Gly Glu Asn Ser Pro Leu Leu Ser Gly 
1               5                   


<210>  104
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  104

Glu Glu Lys Gln Pro Leu Leu Met Glu 
1               5                   


<210>  105
<211>  9
<212>  PRT
<213>  Oryzias latipes

<400>  105

Gly Glu Arg Gln Pro Leu Leu Gln Ser 
1               5                   


<210>  106
<211>  9
<212>  PRT
<213>  Gallus gallus

<400>  106

Pro Glu Ile Gln Pro Leu Leu Thr Glu 
1               5                   


<210>  107
<211>  9
<212>  PRT
<213>  Carassius auratus

<400>  107

Glu Gly Arg Gln Pro Leu Leu Gly Asp 
1               5                   


<210>  108
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  108

Glu Ala Asn Gln Pro Leu Leu Thr Asp 
1               5                   


<210>  109
<211>  9
<212>  PRT
<213>  Gallus gallus

<400>  109

Glu Leu His Gln Pro Leu Leu Thr Asp 
1               5                   


<210>  110
<211>  9
<212>  PRT
<213>  Danio rerio

<400>  110

Arg Glu Phe Glu Pro Leu Leu Asn Ala 
1               5                   


<210>  111
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  111

Glu Glu Lys Met Ala Ile Leu Met Asp 
1               5                   


<210>  112
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  112

Glu Glu Lys Leu Ala Ile Leu Ser Gln 
1               5                   


<210>  113
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  113

Ser Glu Arg Asp Val Leu Leu Asp Glu 
1               5                   


<210>  114
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  114

Ser Glu Arg Arg Asn Leu Leu Glu Asp 
1               5                   


<210>  115
<211>  9
<212>  PRT
<213>  Rattus norvegicus

<400>  115

Asp Asp Ser Gly Asp Leu Leu Pro Gly 
1               5                   


<210>  116
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  116

Ser Gln Ile Lys Arg Leu Leu Ser Glu 
1               5                   


<210>  117
<211>  9
<212>  PRT
<213>  Felis catus

<400>  117

Ser His Ile Lys Arg Leu Leu Ser Glu 
1               5                   


<210>  118
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  118

Arg Arg Thr Pro Ser Leu Leu Glu Gln 
1               5                   


<210>  119
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  119

His Arg Thr Pro Ser Leu Leu Glu Gln 
1               5                   


<210>  120
<211>  10
<212>  PRT
<213>  Rattus norvegicus

<400>  120

Glu Pro Arg Gly Ser Arg Leu Leu Val Arg 
1               5                   10  


<210>  121
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  121

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn Asn Glu Gln Leu Pro Met 
1               5                   10                  15      


Leu Gly Arg 
            


<210>  122
<211>  19
<212>  PRT
<213>  Mus musculus

<400>  122

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn His Glu Gln Leu Pro Ile 
1               5                   10                  15      


Leu Gly Asn 
            


<210>  123
<211>  19
<212>  PRT
<213>  Gallus gallus

<400>  123

Met Asp Asp Gln Arg Asp Leu Ile Ser Asn His Glu Gln Leu Pro Ile 
1               5                   10                  15      


Leu Gly Asn 
            


<210>  124
<211>  23
<212>  PRT
<213>  Danio rerio

<400>  124

Met Glu Pro Asp His Gln Asn Glu Ser Leu Ile Gln Arg Val Pro Ser 
1               5                   10                  15      


Ala Glu Thr Ile Leu Gly Arg 
            20              


<210>  125
<211>  23
<212>  PRT
<213>  Danio rerio

<400>  125

Met Ser Ser Glu Gly Asn Glu Thr Pro Leu Ile Ser Asp Gln Ser Ser 
1               5                   10                  15      


Val Asn Met Gly Pro Gln Pro 
            20              


<210>  126
<211>  24
<212>  PRT
<213>  Trypanosoma sp.

<400>  126

Arg Pro Arg Arg Arg Thr Glu Glu Asp Glu Leu Leu Pro Glu Glu Ala 
1               5                   10                  15      


Glu Gly Leu Ile Asp Pro Gln Asn 
            20                  


<210>  127
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  127

Pro Asp Lys His Ser Leu Leu Val Gly Asp Phe Arg Glu Asp Asp Asp 
1               5                   10                  15      


Thr Ala Leu 
            


<210>  128
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  128

Thr Glu Arg Glu Arg Leu Leu Asn Phe 
1               5                   


<210>  129
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  129

Val Glu Thr Asp Asp Leu Ile Leu 
1               5               


<210>  130
<211>  7
<212>  PRT
<213>  Caenorhabditis elegans

<400>  130

Phe Glu Asn Asp Ser Leu Leu 
1               5           


<210>  131
<211>  9
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  131

Asn Glu Gln Ser Pro Leu Leu His Asn 
1               5                   


<210>  132
<211>  8
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  132

Ser Glu Gln Thr Arg Leu Val Pro 
1               5               


<210>  133
<211>  8
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  133

Glu Val Asp Leu Asp Leu Leu Lys 
1               5               


<210>  134
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dileucine-based sorting signal sequence


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  134

Asp Xaa Xaa Leu Leu 
1               5   


<210>  135
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  135

Ser Phe His Asp Asp Ser Asp Glu Asp Leu Leu His Ile 
1               5                   10              


<210>  136
<211>  13
<212>  PRT
<213>  Bos taurus

<400>  136

Thr Phe His Asp Asp Ser Asp Glu Asp Leu Leu His Val 
1               5                   10              


<210>  137
<211>  13
<212>  PRT
<213>  Oryctolagus cuniculus

<400>  137

Ser Phe His Asp Asp Ser Asp Glu Asp Leu Leu Asn Ile 
1               5                   10              


<210>  138
<211>  13
<212>  PRT
<213>  Gallus gallus

<400>  138

Ser Phe His Asp Asp Ser Asp Glu Asp Leu Leu Asn Val 
1               5                   10              


<210>  139
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  139

Glu Glu Ser Glu Glu Arg Asp Asp His Leu Leu Pro Met 
1               5                   10              


<210>  140
<211>  13
<212>  PRT
<213>  Gallus gallus

<400>  140

Asp Glu Ser Glu Glu Arg Asp Asp His Leu Leu Pro Met 
1               5                   10              


<210>  141
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  141

Gly Tyr His Asp Asp Ser Asp Glu Asp Leu Leu Glu 
1               5                   10          


<210>  142
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  142

Ile Thr Gly Phe Ser Asp Asp Val Pro Met Val Ile Ala 
1               5                   10              


<210>  143
<211>  13
<212>  PRT
<213>  Hydra sp.

<400>  143

Ile Asn Arg Phe Ser Asp Asp Glu Pro Leu Val Val Ala 
1               5                   10              


<210>  144
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  144

Met Leu Glu Ala Ser Asp Asp Glu Ala Leu Leu Val Cys 
1               5                   10              


<210>  145
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  145

Lys Asn Glu Thr Ser Asp Asp Glu Ala Leu Leu Leu Cys 
1               5                   10              


<210>  146
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  146

Trp Val Val Glu Ala Glu Asp Glu Pro Leu Leu Ala 
1               5                   10          


<210>  147
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  147

Trp Val Ala Glu Ala Glu Asp Glu Pro Leu Leu Thr 
1               5                   10          


<210>  148
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  148

His Asp Asp Phe Ala Asp Asp Ile Ser Leu Leu Lys 
1               5                   10          


<210>  149
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  149

Gly Arg Asp Ser Pro Glu Asp His Ser Leu Leu Val Asn 
1               5                   10              


<210>  150
<211>  12
<212>  PRT
<213>  Peromyscus maniculatus

<400>  150

Val Arg Cys His Pro Glu Asp Asp Arg Leu Leu Gly 
1               5                   10          


<210>  151
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  151

His Arg Val Ser Gln Asp Asp Leu Asp Leu Leu Thr Ser 
1               5                   10              


<210>  152
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  152

Ala Ser Val Ser Leu Leu Asp Asp Glu Leu Met Ser Leu 
1               5                   10              


<210>  153
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  153

Ala Ser Ser Gly Leu Asp Asp Leu Asp Leu Leu Gly Lys 
1               5                   10              


<210>  154
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  154

Val Gln Asn Pro Ser Ala Asp Arg Asn Leu Leu Asp Leu 
1               5                   10              


<210>  155
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  155

Asn Ala Leu Ser Trp Leu Asp Glu Glu Leu Leu Cys Leu 
1               5                   10              


<210>  156
<211>  13
<212>  PRT
<213>  Drosophila sp.

<400>  156

Thr Val Asp Ser Ile Asp Asp Val Pro Leu Leu Ser Asp 
1               5                   10              


<210>  157
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  157

Gln Glu Glu Cys Pro Ser Asp Ser Glu Glu Asp Glu Gly 
1               5                   10              


<210>  158
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  158

Arg Asp Arg Asp Tyr Asp Glu Asp Asp Glu Asp Asp Ile 
1               5                   10              


<210>  159
<211>  15
<212>  PRT
<213>  Mus musculus

<400>  159

Leu Asp Glu Thr Glu Asp Asp Glu Leu Glu Tyr Asp Asp Glu Ser 
1               5                   10                  15  


<210>  160
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  160

Lys Asp Pro Asp Glu Val Glu Thr Glu Ser 
1               5                   10  


<210>  161
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  161

His Glu Phe Gln Asp Glu Thr Asp Thr Glu Glu Glu Thr 
1               5                   10              


<210>  162
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  162

Gln Glu Lys Glu Asp Asp Gly Ser Glu Ser Glu Glu Glu Tyr 
1               5                   10                  


<210>  163
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  163

Gly Glu Asp Glu Glu Ser Glu Ser Asp 
1               5                   


<210>  164
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  164

Gly Glu Asp Ser Asp Glu Glu Pro Asp His Glu Glu 
1               5                   10          


<210>  165
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  165

Leu Glu Asp Asp Ser Asp Glu Glu Glu Asp Phe 
1               5                   10      


<210>  166
<211>  9
<212>  PRT
<213>  Human cytomegalovirus

<400>  166

Lys Asp Ser Asp Glu Glu Glu Asn Val 
1               5                   


<210>  167
<211>  13
<212>  PRT
<213>  Herpes simplex virus 3

<400>  167

Phe Glu Asp Ser Glu Ser Thr Asp Thr Glu Glu Glu Phe 
1               5                   10              


<210>  168
<211>  9
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  168

Leu Glu Ala Gln Glu Glu Glu Glu Val 
1               5                   


<210>  169
<211>  22
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  169

Ala Asp Asp Leu Glu Ser Gly Leu Gly Ala Glu Asp Asp Leu Glu Gln 
1               5                   10                  15      


Asp Glu Gln Leu Glu Gly 
            20          


<210>  170
<211>  12
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  170

Thr Glu Ile Asp Glu Ser Phe Glu Met Thr Asp Phe 
1               5                   10          


<210>  171
<211>  18
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  171

Thr Glu Pro Glu Glu Val Glu Asp Phe Asp Phe Asp Leu Ser Asp Glu 
1               5                   10                  15      


Asp His 
        


<210>  172
<211>  15
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  172

Phe Glu Ile Glu Glu Asp Asp Val Pro Thr Leu Glu Glu Glu His 
1               5                   10                  15  


<210>  173
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal sequence


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Phe or Trp

<400>  173

Asp Pro Xaa 
1           


<210>  174
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  174

Phe Xaa Asp Xaa Phe 
1               5   


<210>  175
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is an amino acid with a bulky hydrophobic side chain

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is an amino acid with a bulky hydrophobic side chain

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa may be Asp or Glu

<400>  175

Leu Xaa Xaa Xaa Xaa 
1               5   


<210>  176
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal sequence

<400>  176

Leu Leu Asp Leu Leu 
1               5   


<210>  177
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal sequence

<400>  177

Pro Trp Asp Leu Trp 
1               5   


<210>  178
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular retention signal sequence

<400>  178

His Asp Glu Leu 
1               


<210>  179
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM (immunoreceptor tyrosine-based activation motif)


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa may be Leu or Ile

<400>  179

Tyr Xaa Xaa Xaa 
1               


<210>  180
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  180

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


