                         SEQUENCE LISTING

<110>  Randox Laboratories Ltd.
 
<120>  XBP1 ISOFORM MULTIPLEX ASSAY

<130>  JWJ02906EP

<150>  GB 1914517.6
<151>  2019-10-08

<150>  PCT/EP2020/078174
<151>  2020-10-07

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  261
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Val Val Val Ala Ala Ala Pro Asn Pro Ala Asp Gly Thr Pro Lys 
1               5                   10                  15      


Val Leu Leu Leu Ser Gly Gln Pro Ala Ser Ala Ala Gly Ala Pro Ala 
            20                  25                  30          


Gly Gln Ala Leu Pro Leu Met Val Pro Ala Gln Arg Gly Ala Ser Pro 
        35                  40                  45              


Glu Ala Ala Ser Gly Gly Leu Pro Gln Ala Arg Lys Arg Gln Arg Leu 
    50                  55                  60                  


Thr His Leu Ser Pro Glu Glu Lys Ala Leu Arg Arg Lys Leu Lys Asn 
65                  70                  75                  80  


Arg Val Ala Ala Gln Thr Ala Arg Asp Arg Lys Lys Ala Arg Met Ser 
                85                  90                  95      


Glu Leu Glu Gln Gln Val Val Asp Leu Glu Glu Glu Asn Gln Lys Leu 
            100                 105                 110         


Leu Leu Glu Asn Gln Leu Leu Arg Glu Lys Thr His Gly Leu Val Val 
        115                 120                 125             


Glu Asn Gln Glu Leu Arg Gln Arg Leu Gly Met Asp Ala Leu Val Ala 
    130                 135                 140                 


Glu Glu Glu Ala Glu Ala Lys Gly Asn Glu Val Arg Pro Val Ala Gly 
145                 150                 155                 160 


Ser Ala Glu Ser Ala Ala Leu Arg Leu Arg Ala Pro Leu Gln Gln Val 
                165                 170                 175     


Gln Ala Gln Leu Ser Pro Leu Gln Asn Ile Ser Pro Trp Ile Leu Ala 
            180                 185                 190         


Val Leu Thr Leu Gln Ile Gln Ser Leu Ile Ser Cys Trp Ala Phe Trp 
        195                 200                 205             


Thr Thr Trp Thr Gln Ser Cys Ser Ser Asn Ala Leu Pro Gln Ser Leu 
    210                 215                 220                 


Pro Ala Trp Arg Ser Ser Gln Arg Ser Thr Gln Lys Asp Pro Val Pro 
225                 230                 235                 240 


Tyr Gln Pro Pro Phe Leu Cys Gln Trp Gly Arg His Gln Pro Ser Trp 
                245                 250                 255     


Lys Pro Leu Met Asn 
            260     


<210>  2
<211>  376
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Val Val Val Ala Ala Ala Pro Asn Pro Ala Asp Gly Thr Pro Lys 
1               5                   10                  15      


Val Leu Leu Leu Ser Gly Gln Pro Ala Ser Ala Ala Gly Ala Pro Ala 
            20                  25                  30          


Gly Gln Ala Leu Pro Leu Met Val Pro Ala Gln Arg Gly Ala Ser Pro 
        35                  40                  45              


Glu Ala Ala Ser Gly Gly Leu Pro Gln Ala Arg Lys Arg Gln Arg Leu 
    50                  55                  60                  


Thr His Leu Ser Pro Glu Glu Lys Ala Leu Arg Arg Lys Leu Lys Asn 
65                  70                  75                  80  


Arg Val Ala Ala Gln Thr Ala Arg Asp Arg Lys Lys Ala Arg Met Ser 
                85                  90                  95      


Glu Leu Glu Gln Gln Val Val Asp Leu Glu Glu Glu Asn Gln Lys Leu 
            100                 105                 110         


Leu Leu Glu Asn Gln Leu Leu Arg Glu Lys Thr His Gly Leu Val Val 
        115                 120                 125             


Glu Asn Gln Glu Leu Arg Gln Arg Leu Gly Met Asp Ala Leu Val Ala 
    130                 135                 140                 


Glu Glu Glu Ala Glu Ala Lys Gly Asn Glu Val Arg Pro Val Ala Gly 
145                 150                 155                 160 


Ser Ala Glu Ser Ala Ala Gly Ala Gly Pro Val Val Thr Pro Pro Glu 
                165                 170                 175     


His Leu Pro Met Asp Ser Gly Gly Ile Asp Ser Ser Asp Ser Glu Ser 
            180                 185                 190         


Asp Ile Leu Leu Gly Ile Leu Asp Asn Leu Asp Pro Val Met Phe Phe 
        195                 200                 205             


Lys Cys Pro Ser Pro Glu Pro Ala Ser Leu Glu Glu Leu Pro Glu Val 
    210                 215                 220                 


Tyr Pro Glu Gly Pro Ser Ser Leu Pro Ala Ser Leu Ser Leu Ser Val 
225                 230                 235                 240 


Gly Thr Ser Ser Ala Lys Leu Glu Ala Ile Asn Glu Leu Ile Arg Phe 
                245                 250                 255     


Asp His Ile Tyr Thr Lys Pro Leu Val Leu Glu Ile Pro Ser Glu Thr 
            260                 265                 270         


Glu Ser Gln Ala Asn Val Val Val Lys Ile Glu Glu Ala Pro Leu Ser 
        275                 280                 285             


Pro Ser Glu Asn Asp His Pro Glu Phe Ile Val Ser Val Lys Glu Glu 
    290                 295                 300                 


Pro Val Glu Asp Asp Leu Val Pro Glu Leu Gly Ile Ser Asn Leu Leu 
305                 310                 315                 320 


Ser Ser Ser His Cys Pro Lys Pro Ser Ser Cys Leu Leu Asp Ala Tyr 
                325                 330                 335     


Ser Asp Cys Gly Tyr Gly Gly Ser Leu Ser Pro Phe Ser Asp Met Ser 
            340                 345                 350         


Ser Leu Leu Gly Val Asn His Ser Trp Glu Asp Thr Phe Ala Asn Glu 
        355                 360                 365             


Leu Phe Pro Gln Leu Ile Ser Val 
    370                 375     


<210>  3
<211>  95
<212>  PRT
<213>  Homo sapiens

<400>  3

Leu Arg Leu Arg Ala Pro Leu Gln Gln Val Gln Ala Gln Leu Ser Pro 
1               5                   10                  15      


Leu Gln Asn Ile Ser Pro Trp Ile Leu Ala Val Leu Thr Leu Gln Ile 
            20                  25                  30          


Gln Ser Leu Ile Ser Cys Trp Ala Phe Trp Thr Thr Trp Thr Gln Ser 
        35                  40                  45              


Cys Ser Ser Asn Ala Leu Pro Gln Ser Leu Pro Ala Trp Arg Ser Ser 
    50                  55                  60                  


Gln Arg Ser Thr Gln Lys Asp Pro Val Pro Tyr Gln Pro Pro Phe Leu 
65                  70                  75                  80  


Cys Gln Trp Gly Arg His Gln Pro Ser Trp Lys Pro Leu Met Asn 
                85                  90                  95  


<210>  4
<211>  210
<212>  PRT
<213>  Homo sapiens

<400>  4

Gly Ala Gly Pro Val Val Thr Pro Pro Glu His Leu Pro Met Asp Ser 
1               5                   10                  15      


Gly Gly Ile Asp Ser Ser Asp Ser Glu Ser Asp Ile Leu Leu Gly Ile 
            20                  25                  30          


Leu Asp Asn Leu Asp Pro Val Met Phe Phe Lys Cys Pro Ser Pro Glu 
        35                  40                  45              


Pro Ala Ser Leu Glu Glu Leu Pro Glu Val Tyr Pro Glu Gly Pro Ser 
    50                  55                  60                  


Ser Leu Pro Ala Ser Leu Ser Leu Ser Val Gly Thr Ser Ser Ala Lys 
65                  70                  75                  80  


Leu Glu Ala Ile Asn Glu Leu Ile Arg Phe Asp His Ile Tyr Thr Lys 
                85                  90                  95      


Pro Leu Val Leu Glu Ile Pro Ser Glu Thr Glu Ser Gln Ala Asn Val 
            100                 105                 110         


Val Val Lys Ile Glu Glu Ala Pro Leu Ser Pro Ser Glu Asn Asp His 
        115                 120                 125             


Pro Glu Phe Ile Val Ser Val Lys Glu Glu Pro Val Glu Asp Asp Leu 
    130                 135                 140                 


Val Pro Glu Leu Gly Ile Ser Asn Leu Leu Ser Ser Ser His Cys Pro 
145                 150                 155                 160 


Lys Pro Ser Ser Cys Leu Leu Asp Ala Tyr Ser Asp Cys Gly Tyr Gly 
                165                 170                 175     


Gly Ser Leu Ser Pro Phe Ser Asp Met Ser Ser Leu Leu Gly Val Asn 
            180                 185                 190         


His Ser Trp Glu Asp Thr Phe Ala Asn Glu Leu Phe Pro Gln Leu Ile 
        195                 200                 205             


Ser Val 
    210 


<210>  5
<211>  166
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Val Val Val Ala Ala Ala Pro Asn Pro Ala Asp Gly Thr Pro Lys 
1               5                   10                  15      


Val Leu Leu Leu Ser Gly Gln Pro Ala Ser Ala Ala Gly Ala Pro Ala 
            20                  25                  30          


Gly Gln Ala Leu Pro Leu Met Val Pro Ala Gln Arg Gly Ala Ser Pro 
        35                  40                  45              


Glu Ala Ala Ser Gly Gly Leu Pro Gln Ala Arg Lys Arg Gln Arg Leu 
    50                  55                  60                  


Thr His Leu Ser Pro Glu Glu Lys Ala Leu Arg Arg Lys Leu Lys Asn 
65                  70                  75                  80  


Arg Val Ala Ala Gln Thr Ala Arg Asp Arg Lys Lys Ala Arg Met Ser 
                85                  90                  95      


Glu Leu Glu Gln Gln Val Val Asp Leu Glu Glu Glu Asn Gln Lys Leu 
            100                 105                 110         


Leu Leu Glu Asn Gln Leu Leu Arg Glu Lys Thr His Gly Leu Val Val 
        115                 120                 125             


Glu Asn Gln Glu Leu Arg Gln Arg Leu Gly Met Asp Ala Leu Val Ala 
    130                 135                 140                 


Glu Glu Glu Ala Glu Ala Lys Gly Asn Glu Val Arg Pro Val Ala Gly 
145                 150                 155                 160 


Ser Ala Glu Ser Ala Ala 
                165     


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer 1, XBP1

<400>  6
ggaacagcaa gtggtaga                                                     18


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer 1, XBP1

<400>  7
ctggaggggt gacaactg                                                     18


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer 2, GAPDH

<400>  8
accacagtcc atgccatc                                                     18


<210>  9
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer 2, GAPDH

<400>  9
tccaccaccc tgttgctg                                                     18


