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<110>  ɶʢ＼޹˾
             ɶʢ＼޹˾
 
<120>  NKG2D CAR-TϸƱӦ

<130>  LJH002

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  350
<212>  PRT
<213>  ˹

<220>
<223>  N92 CAR 

<400>  1

Thr Glu Ser Tyr Cys Gly Pro Cys Pro Lys Asn Trp Ile Cys Tyr Lys 
1               5                   10                  15      


Asn Asn Cys Tyr Gln Phe Phe Asp Glu Ser Lys Asn Trp Tyr Glu Ser 
            20                  25                  30          


Gln Ala Ser Cys Met Ser Gln Asn Ala Ser Leu Leu Lys Val Tyr Ser 
        35                  40                  45              


Lys Glu Asp Gln Asp Leu Leu Lys Leu Val Lys Ser Tyr His Trp Met 
    50                  55                  60                  


Gly Leu Val His Ile Pro Thr Asn Gly Ser Trp Gln Trp Glu Asp Gly 
65                  70                  75                  80  


Ser Ile Leu Ser Pro Asn Leu Leu Thr Ile Ile Glu Met Gln Lys Gly 
                85                  90                  95      


Asp Cys Ala Leu Tyr Ala Ser Ser Phe Lys Gly Tyr Ile Glu Asn Cys 
            100                 105                 110         


Ser Thr Pro Asn Thr Tyr Ile Cys Met Gln Arg Thr Val Glu Phe Thr 
        115                 120                 125             


Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
    130                 135                 140                 


Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
145                 150                 155                 160 


Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp 
                165                 170                 175     


Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile 
            180                 185                 190         


Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys 
        195                 200                 205             


Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys 
    210                 215                 220                 


Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val 
225                 230                 235                 240 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
                245                 250                 255     


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
            260                 265                 270         


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
        275                 280                 285             


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
    290                 295                 300                 


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
305                 310                 315                 320 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
                325                 330                 335     


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            340                 345                 350 


<210>  2
<211>  1050
<212>  DNA
<213>  ˹

<220>
<223>  N92 CAR DNA

<400>  2
accgaaagtt actgtggccc atgtcctaaa aactggatat gttacaaaaa taactgctac       60

caattttttg atgagagtaa aaactggtat gagagccagg cttcttgtat gtctcaaaat      120

gccagccttc tgaaagtata cagcaaagag gaccaggatt tacttaaact ggtgaagtca      180

tatcattgga tgggactagt acacattcca acaaatggat cttggcagtg ggaagatggc      240

tccattctct cacccaacct actaacaata attgaaatgc agaagggaga ctgtgcactc      300

tatgcctcga gctttaaagg ctatatagaa aactgttcaa ctccaaatac atacatctgc      360

atgcaaagga ctgtggaatt caccacgacg ccagcgccgc gaccaccaac accggcgccc      420

accatcgcgt cgcagcccct gtccctgcgc ccagaggcgt gccggccagc ggcggggggc      480

gcagtgcaca cgagggggct ggacttcgcc tgtgatatct acatctgggc gcccctggcc      540

gggacttgtg gggtccttct cctgtcactg gttatcaccc tttactgcaa acggggcaga      600

aagaaactcc tgtatatatt caaacaacca tttatgagac cagtacaaac tactcaagag      660

gaagatggct gtagctgccg atttccagaa gaagaagaag gaggatgtga actgagagtg      720

aagttcagca ggagcgcaga cgcccccgcg taccagcagg gccagaacca gctctataac      780

gagctcaatc taggacgaag agaggagtac gatgttttgg acaagagacg tggccgggac      840

cctgagatgg ggggaaagcc gagaaggaag aaccctcagg aaggcctgta caatgaactg      900

cagaaagata agatggcgga ggcctacagt gagattggga tgaaaggcga gcgccggagg      960

ggcaaggggc acgatggcct ttaccagggt ctcagtacag ccaccaagga cacctacgac     1020

gcccttcaca tgcaggccct gccccctcgc                                      1050


<210>  3
<211>  125
<212>  PRT
<213>  ˹

<220>
<223>  N92 (NKG2D 92-216) 

<400>  3

Thr Glu Ser Tyr Cys Gly Pro Cys Pro Lys Asn Trp Ile Cys Tyr Lys 
1               5                   10                  15      


Asn Asn Cys Tyr Gln Phe Phe Asp Glu Ser Lys Asn Trp Tyr Glu Ser 
            20                  25                  30          


Gln Ala Ser Cys Met Ser Gln Asn Ala Ser Leu Leu Lys Val Tyr Ser 
        35                  40                  45              


Lys Glu Asp Gln Asp Leu Leu Lys Leu Val Lys Ser Tyr His Trp Met 
    50                  55                  60                  


Gly Leu Val His Ile Pro Thr Asn Gly Ser Trp Gln Trp Glu Asp Gly 
65                  70                  75                  80  


Ser Ile Leu Ser Pro Asn Leu Leu Thr Ile Ile Glu Met Gln Lys Gly 
                85                  90                  95      


Asp Cys Ala Leu Tyr Ala Ser Ser Phe Lys Gly Tyr Ile Glu Asn Cys 
            100                 105                 110         


Ser Thr Pro Asn Thr Tyr Ile Cys Met Gln Arg Thr Val 
        115                 120                 125 


<210>  4
<211>  375
<212>  DNA
<213>  ˹

<220>
<223>  N92 (NKG2D 92-216) DNA

<400>  4
accgaaagtt actgtggccc atgtcctaaa aactggatat gttacaaaaa taactgctac       60

caattttttg atgagagtaa aaactggtat gagagccagg cttcttgtat gtctcaaaat      120

gccagccttc tgaaagtata cagcaaagag gaccaggatt tacttaaact ggtgaagtca      180

tatcattgga tgggactagt acacattcca acaaatggat cttggcagtg ggaagatggc      240

tccattctct cacccaacct actaacaata attgaaatgc agaagggaga ctgtgcactc      300

tatgcctcga gctttaaagg ctatatagaa aactgttcaa ctccaaatac atacatctgc      360

atgcaaagga ctgtg                                                       375


