                         SEQUENCE LISTING

<110>  CERo Therapeutics, Inc.
 
<120>  CHIMERIC TIM4 RECEPTORS AND USES THEREOF

<130>  200265.411WO

<150>  62/910,133
<151>  2019-10-03

<150>  63/066,098
<151>  2020-08-14

<160>  109   

<170>  PatentIn version 3.5

<210>  1
<211>  378
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ser Lys Glu Pro Leu Ile Leu Trp Leu Met Ile Glu Phe Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Val Thr Ser Glu Thr Val Val Thr Glu Val Leu 
            20                  25                  30          


Gly His Arg Val Thr Leu Pro Cys Leu Tyr Ser Ser Trp Ser His Asn 
        35                  40                  45              


Ser Asn Ser Met Cys Trp Gly Lys Asp Gln Cys Pro Tyr Ser Gly Cys 
    50                  55                  60                  


Lys Glu Ala Leu Ile Arg Thr Asp Gly Met Arg Val Thr Ser Arg Lys 
65                  70                  75                  80  


Ser Ala Lys Tyr Arg Leu Gln Gly Thr Ile Pro Arg Gly Asp Val Ser 
                85                  90                  95      


Leu Thr Ile Leu Asn Pro Ser Glu Ser Asp Ser Gly Val Tyr Cys Cys 
            100                 105                 110         


Arg Ile Glu Val Pro Gly Trp Phe Asn Asp Val Lys Ile Asn Val Arg 
        115                 120                 125             


Leu Asn Leu Gln Arg Ala Ser Thr Thr Thr His Arg Thr Ala Thr Thr 
    130                 135                 140                 


Thr Thr Arg Arg Thr Thr Thr Thr Ser Pro Thr Thr Thr Arg Gln Met 
145                 150                 155                 160 


Thr Thr Thr Pro Ala Ala Leu Pro Thr Thr Val Val Thr Thr Pro Asp 
                165                 170                 175     


Leu Thr Thr Gly Thr Pro Leu Gln Met Thr Thr Ile Ala Val Phe Thr 
            180                 185                 190         


Thr Ala Asn Thr Cys Leu Ser Leu Thr Pro Ser Thr Leu Pro Glu Glu 
        195                 200                 205             


Ala Thr Gly Leu Leu Thr Pro Glu Pro Ser Lys Glu Gly Pro Ile Leu 
    210                 215                 220                 


Thr Ala Glu Ser Glu Thr Val Leu Pro Ser Asp Ser Trp Ser Ser Val 
225                 230                 235                 240 


Glu Ser Thr Ser Ala Asp Thr Val Leu Leu Thr Ser Lys Glu Ser Lys 
                245                 250                 255     


Val Trp Asp Leu Pro Ser Thr Ser His Val Ser Met Trp Lys Thr Ser 
            260                 265                 270         


Asp Ser Val Ser Ser Pro Gln Pro Gly Ala Ser Asp Thr Ala Val Pro 
        275                 280                 285             


Glu Gln Asn Lys Thr Thr Lys Thr Gly Gln Met Asp Gly Ile Pro Met 
    290                 295                 300                 


Ser Met Lys Asn Glu Met Pro Ile Ser Gln Leu Leu Met Ile Ile Ala 
305                 310                 315                 320 


Pro Ser Leu Gly Phe Val Leu Phe Ala Leu Phe Val Ala Phe Leu Leu 
                325                 330                 335     


Arg Gly Lys Leu Met Glu Thr Tyr Cys Ser Gln Lys His Thr Arg Leu 
            340                 345                 350         


Asp Tyr Ile Gly Asp Ser Lys Asn Val Leu Asn Asp Val Gln His Gly 
        355                 360                 365             


Arg Glu Asp Glu Asp Gly Leu Phe Thr Leu 
    370                 375             


<210>  2
<211>  314
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SIGNAL
<222>  (1)..(24)

<400>  2

Met Ser Lys Glu Pro Leu Ile Leu Trp Leu Met Ile Glu Phe Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Val Thr Ser Glu Thr Val Val Thr Glu Val Leu 
            20                  25                  30          


Gly His Arg Val Thr Leu Pro Cys Leu Tyr Ser Ser Trp Ser His Asn 
        35                  40                  45              


Ser Asn Ser Met Cys Trp Gly Lys Asp Gln Cys Pro Tyr Ser Gly Cys 
    50                  55                  60                  


Lys Glu Ala Leu Ile Arg Thr Asp Gly Met Arg Val Thr Ser Arg Lys 
65                  70                  75                  80  


Ser Ala Lys Tyr Arg Leu Gln Gly Thr Ile Pro Arg Gly Asp Val Ser 
                85                  90                  95      


Leu Thr Ile Leu Asn Pro Ser Glu Ser Asp Ser Gly Val Tyr Cys Cys 
            100                 105                 110         


Arg Ile Glu Val Pro Gly Trp Phe Asn Asp Val Lys Ile Asn Val Arg 
        115                 120                 125             


Leu Asn Leu Gln Arg Ala Ser Thr Thr Thr His Arg Thr Ala Thr Thr 
    130                 135                 140                 


Thr Thr Arg Arg Thr Thr Thr Thr Ser Pro Thr Thr Thr Arg Gln Met 
145                 150                 155                 160 


Thr Thr Thr Pro Ala Ala Leu Pro Thr Thr Val Val Thr Thr Pro Asp 
                165                 170                 175     


Leu Thr Thr Gly Thr Pro Leu Gln Met Thr Thr Ile Ala Val Phe Thr 
            180                 185                 190         


Thr Ala Asn Thr Cys Leu Ser Leu Thr Pro Ser Thr Leu Pro Glu Glu 
        195                 200                 205             


Ala Thr Gly Leu Leu Thr Pro Glu Pro Ser Lys Glu Gly Pro Ile Leu 
    210                 215                 220                 


Thr Ala Glu Ser Glu Thr Val Leu Pro Ser Asp Ser Trp Ser Ser Val 
225                 230                 235                 240 


Glu Ser Thr Ser Ala Asp Thr Val Leu Leu Thr Ser Lys Glu Ser Lys 
                245                 250                 255     


Val Trp Asp Leu Pro Ser Thr Ser His Val Ser Met Trp Lys Thr Ser 
            260                 265                 270         


Asp Ser Val Ser Ser Pro Gln Pro Gly Ala Ser Asp Thr Ala Val Pro 
        275                 280                 285             


Glu Gln Asn Lys Thr Thr Lys Thr Gly Gln Met Asp Gly Ile Pro Met 
    290                 295                 300                 


Ser Met Lys Asn Glu Met Pro Ile Ser Gln 
305                 310                 


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified IgG4 hinge region

<400>  3

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  4
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  4

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  5
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  5

Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His 
1               5                   10                  15      


Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln 
            20                  25                  30          


Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
        35                  40          


<210>  6
<211>  116
<212>  PRT
<213>  Homo sapiens

<400>  6

Lys Arg Lys Lys Gln Arg Ser Arg Arg Asn Asp Glu Glu Leu Glu Thr 
1               5                   10                  15      


Arg Ala His Arg Val Ala Thr Glu Glu Arg Gly Arg Lys Pro His Gln 
            20                  25                  30          


Ile Pro Ala Ser Thr Pro Gln Asn Pro Ala Thr Ser Gln His Pro Pro 
        35                  40                  45              


Pro Pro Pro Gly His Arg Ser Gln Ala Pro Ser His Arg Pro Pro Pro 
    50                  55                  60                  


Pro Gly His Arg Val Gln His Gln Pro Gln Lys Arg Pro Pro Ala Pro 
65                  70                  75                  80  


Ser Gly Thr Gln Val His Gln Gln Lys Gly Pro Pro Leu Pro Arg Pro 
                85                  90                  95      


Arg Val Gln Pro Lys Pro Pro His Gly Ala Ala Glu Asn Ser Leu Ser 
            100                 105                 110         


Pro Ser Ser Asn 
        115     


<210>  7
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  7

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  8
<211>  48
<212>  PRT
<213>  Homo sapiens

<400>  8

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  9
<211>  81
<212>  PRT
<213>  Homo sapiens

<400>  9

Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala 
1               5                   10                  15      


Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu 
            20                  25                  30          


Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg 
        35                  40                  45              


Pro Tyr Tyr Lys Asn Arg Gln Arg Lys Ile Lys Lys Tyr Arg Leu Gln 
    50                  55                  60                  


Gln Ala Gln Lys Gly Thr Pro Met Lys Pro Asn Thr Gln Ala Thr Pro 
65                  70                  75                  80  


Pro 
    


<210>  10
<211>  46
<212>  PRT
<213>  Homo sapiens

<400>  10

Lys Ala Leu Ile His Leu Ser Asp Leu Arg Glu Tyr Arg Arg Phe Glu 
1               5                   10                  15      


Lys Glu Lys Leu Lys Ser Gln Trp Asn Asn Asp Asn Pro Leu Phe Lys 
            20                  25                  30          


Ser Ala Thr Thr Thr Val Met Asn Pro Lys Phe Ala Glu Ser 
        35                  40                  45      


<210>  11
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  11

Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp Pro Asn 
1               5                   10                  15      


Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys Ser Arg 
            20                  25                  30          


Leu Thr Asp Val Thr Leu 
        35              


<210>  12
<211>  188
<212>  PRT
<213>  Homo sapiens

<400>  12

His Arg Arg Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu Cys 
1               5                   10                  15      


Tyr Pro Val Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser Arg 
            20                  25                  30          


Pro Arg Arg Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr Glu 
        35                  40                  45              


Pro Val Ala Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu Thr 
    50                  55                  60                  


Cys His Ser Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp 
65                  70                  75                  80  


Ala Ser Pro Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro 
                85                  90                  95      


Arg Val Ser Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile 
            100                 105                 110         


Met Lys Ala Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu Pro 
        115                 120                 125             


Glu Gly Arg Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu 
    130                 135                 140                 


Leu Glu Ala Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu Pro 
145                 150                 155                 160 


Pro Leu Gly Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu Gly 
                165                 170                 175     


Lys Glu Asp Pro Leu Pro Thr Ala Ala Ser Gly Lys 
            180                 185             


<210>  13
<211>  62
<212>  PRT
<213>  Homo sapiens

<400>  13

Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys Gln 
1               5                   10                  15      


Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr 
            20                  25                  30          


Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln 
        35                  40                  45              


Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln 
    50                  55                  60          


<210>  14
<211>  97
<212>  PRT
<213>  Homo sapiens

<400>  14

Cys Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln 
1               5                   10                  15      


Pro Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr 
            20                  25                  30          


Gly Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val 
        35                  40                  45              


Pro Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser 
    50                  55                  60                  


Gly Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro 
65                  70                  75                  80  


Arg Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro 
                85                  90                  95      


Leu 
    


<210>  15
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  15

Arg Thr Gln Ile Lys Lys Leu Cys Ser Trp Arg Asp Lys Asn Ser Ala 
1               5                   10                  15      


Ala Cys Val Val Tyr Glu Asp Met Ser His Ser Arg Cys Asn Thr Leu 
            20                  25                  30          


Ser Ser Pro Asn Gln Tyr Gln 
        35                  


<210>  16
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Glu Glu Ser Val Val Arg Pro Ser Val Phe Val Val Asp Gly Gln 
1               5                   10                  15      


Thr Asp Ile Pro Phe Thr Arg Leu Gly Arg Ser His Arg Arg Gln Ser 
            20                  25                  30          


Cys Ser Val Ala Arg 
        35          


<210>  17
<211>  70
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Ser Lys Gln Arg Gly Thr Phe Ser Glu Val Ser Leu Ala Gln Asp 
1               5                   10                  15      


Pro Lys Arg Gln Gln Arg Lys Pro Lys Gly Asn Lys Ser Ser Ile Ser 
            20                  25                  30          


Gly Thr Glu Gln Glu Ile Phe Gln Val Glu Leu Asn Leu Gln Asn Pro 
        35                  40                  45              


Ser Leu Asn His Gln Gly Ile Asp Lys Ile Tyr Asp Cys Gln Gly Leu 
    50                  55                  60                  


Leu Pro Pro Pro Glu Lys 
65                  70  


<210>  18
<211>  47
<212>  PRT
<213>  Homo sapiens

<400>  18

Cys Trp Arg Lys Ile Lys Gln Ser Cys Glu Glu Glu Asn Ala Gly Ala 
1               5                   10                  15      


Glu Asp Gln Asp Gly Glu Gly Glu Gly Ser Lys Thr Ala Leu Gln Pro 
            20                  25                  30          


Leu Lys His Ser Asp Ser Lys Glu Asp Asp Gly Gln Glu Ile Ala 
        35                  40                  45          


<210>  19
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated human CD3zeta ITAM-containing activating domain

<400>  19

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  20
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  20

Gly Gln Asp Gly Val Arg Gln Ser Arg Ala Ser Asp Lys Gln Thr Leu 
1               5                   10                  15      


Leu Pro Asn Asp Gln Leu Tyr Gln Pro Leu Lys Asp Arg Glu Asp Asp 
            20                  25                  30          


Gln Tyr Ser His Leu Gln Gly Asn Gln Leu Arg Arg Asn 
        35                  40                  45  


<210>  21
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  21

Gly His Glu Thr Gly Arg Leu Ser Gly Ala Ala Asp Thr Gln Ala Leu 
1               5                   10                  15      


Leu Arg Asn Asp Gln Val Tyr Gln Pro Leu Arg Asp Arg Asp Asp Ala 
            20                  25                  30          


Gln Tyr Ser His Leu Gly Gly Asn Trp Ala Arg Asn Lys 
        35                  40                  45  


<210>  22
<211>  55
<212>  PRT
<213>  Homo sapiens

<400>  22

Lys Asn Arg Lys Ala Lys Ala Lys Pro Val Thr Arg Gly Ala Gly Ala 
1               5                   10                  15      


Gly Gly Arg Gln Arg Gly Gln Asn Lys Glu Arg Pro Pro Pro Val Pro 
            20                  25                  30          


Asn Pro Asp Tyr Glu Pro Ile Arg Lys Gly Gln Arg Asp Leu Tyr Ser 
        35                  40                  45              


Gly Leu Asn Gln Arg Arg Ile 
    50                  55  


<210>  23
<211>  93
<212>  PRT
<213>  Homo sapiens

<400>  23

Lys Lys Leu Val Lys Lys Phe Arg Gln Lys Lys Gln Arg Gln Trp Ile 
1               5                   10                  15      


Gly Pro Thr Gly Met Asn Gln Asn Met Ser Phe His Arg Asn His Thr 
            20                  25                  30          


Ala Thr Val Arg Ser His Ala Glu Asn Pro Thr Ala Ser His Val Asp 
        35                  40                  45              


Asn Glu Tyr Ser Gln Pro Pro Arg Asn Ser His Leu Ser Ala Tyr Pro 
    50                  55                  60                  


Ala Leu Glu Gly Ala Leu His Arg Ser Ser Met Gln Pro Asp Asn Ser 
65                  70                  75                  80  


Ser Asp Ser Asp Tyr Asp Leu His Gly Ala Gln Arg Leu 
                85                  90              


<210>  24
<211>  141
<212>  PRT
<213>  Homo sapiens

<400>  24

Lys Leu Gln Arg Arg Trp Lys Arg Thr Gln Ser Gln Gln Gly Leu Gln 
1               5                   10                  15      


Glu Asn Ser Ser Gly Gln Ser Phe Phe Val Arg Asn Lys Lys Val Arg 
            20                  25                  30          


Arg Ala Pro Leu Ser Glu Gly Pro His Ser Leu Gly Cys Tyr Asn Pro 
        35                  40                  45              


Met Met Glu Asp Gly Ile Ser Tyr Thr Thr Leu Arg Phe Pro Glu Met 
    50                  55                  60                  


Asn Ile Pro Arg Thr Gly Asp Ala Glu Ser Ser Glu Met Gln Arg Pro 
65                  70                  75                  80  


Pro Pro Asp Cys Asp Asp Thr Val Thr Tyr Ser Ala Leu His Lys Arg 
                85                  90                  95      


Gln Val Gly Asp Tyr Glu Asn Val Ile Pro Asp Phe Pro Glu Asp Glu 
            100                 105                 110         


Gly Ile His Tyr Ser Glu Leu Ile Gln Phe Gly Val Gly Glu Arg Pro 
        115                 120                 125             


Gln Ala Gln Glu Asn Val Asp Tyr Val Ile Leu Lys His 
    130                 135                 140     


<210>  25
<211>  61
<212>  PRT
<213>  Homo sapiens

<400>  25

Arg Lys Arg Trp Gln Asn Glu Lys Leu Gly Leu Asp Ala Gly Asp Glu 
1               5                   10                  15      


Tyr Glu Asp Glu Asn Leu Tyr Glu Gly Leu Asn Leu Asp Asp Cys Ser 
            20                  25                  30          


Met Tyr Glu Asp Ile Ser Arg Gly Leu Gln Gly Thr Tyr Gln Asp Val 
        35                  40                  45              


Gly Ser Leu Asn Ile Gly Asp Val Gln Leu Glu Lys Pro 
    50                  55                  60      


<210>  26
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  26

Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu Asp Gly Lys Val 
1               5                   10                  15      


Tyr Ile Asn Met Pro Gly Arg Gly 
            20                  


<210>  27
<211>  76
<212>  PRT
<213>  Homo sapiens

<400>  27

Ala Lys Thr Gly Arg Thr Ser Ile Gln Arg Asp Leu Lys Glu Gln Gln 
1               5                   10                  15      


Pro Gln Ala Leu Ala Pro Gly Arg Gly Pro Ser His Ser Ser Ala Phe 
            20                  25                  30          


Ser Met Ser Pro Leu Ser Thr Ala Gln Ala Pro Leu Pro Asn Pro Arg 
        35                  40                  45              


Thr Ala Ala Ser Ile Tyr Glu Glu Leu Leu Lys His Asp Thr Asn Ile 
    50                  55                  60                  


Tyr Cys Arg Met Asp His Lys Ala Glu Val Ala Ser 
65                  70                  75      


<210>  28
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  28

Leu Leu Met Ile Ile Ala Pro Ser Leu Gly Phe Val Leu Phe Ala Leu 
1               5                   10                  15      


Phe Val Ala Phe Leu 
            20      


<210>  29
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  29

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  30
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  30

Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      


Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          


<210>  31
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  31

Val Ala Ala Ile Leu Gly Leu Gly Leu Val Leu Gly Leu Leu Gly Pro 
1               5                   10                  15      


Leu Ala Ile Leu Leu 
            20      


<210>  32
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  32

Ile Leu Val Ile Phe Ser Gly Met Phe Leu Val Phe Thr Leu Ala Gly 
1               5                   10                  15      


Ala Leu Phe Leu His 
            20      


<210>  33
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  33

Phe Trp Leu Pro Ile Gly Cys Ala Ala Phe Val Val Val Cys Ile Leu 
1               5                   10                  15      


Gly Cys Ile Leu Ile 
            20      


<210>  34
<211>  26
<212>  PRT
<213>  Homo sapiens

<400>  34

Ile Tyr Leu Ile Ile Gly Ile Cys Gly Gly Gly Ser Leu Leu Met Val 
1               5                   10                  15      


Phe Val Ala Leu Leu Val Phe Tyr Ile Thr 
            20                  25      


<210>  35
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  35

Ile Ala Ala Ile Val Gly Gly Thr Val Ala Gly Ile Val Leu Ile Gly 
1               5                   10                  15      


Ile Leu Leu Leu Val Ile Trp 
            20              


<210>  36
<211>  28
<212>  PRT
<213>  Homo sapiens

<400>  36

Pro Val Leu Asp Ala Gly Pro Val Leu Phe Trp Val Ile Leu Val Leu 
1               5                   10                  15      


Val Val Val Val Gly Ser Ser Ala Phe Leu Leu Cys 
            20                  25              


<210>  37
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  37

Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile Leu 
1               5                   10                  15      


Leu Val Leu Val Phe Ile 
            20          


<210>  38
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  38

Val Gly Val Val Gly Gly Leu Leu Gly Ser Leu Val Leu Leu Val Trp 
1               5                   10                  15      


Val Leu Ala Val Ile 
            20      


<210>  39
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  39

Ala Ala Leu Ala Val Ile Ser Phe Leu Leu Gly Leu Gly Leu Gly Val 
1               5                   10                  15      


Ala Cys Val Leu Ala 
            20      


<210>  40
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  40

Val Gly Leu Gly Leu Leu Leu Leu Leu Met Gly Ala Gly Leu Ala Val 
1               5                   10                  15      


Gln Gly Trp Phe Leu 
            20      


<210>  41
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  41

Leu Thr Ala Glu Val Leu Gly Ile Ile Cys Ile Val Leu Met Ala Thr 
1               5                   10                  15      


Val Leu Lys Thr Ile Val Leu 
            20              


<210>  42
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  42

Leu Trp Val Thr Val Gly Leu Ser Val Cys Leu Ile Ala Leu Leu Val 
1               5                   10                  15      


Ala Leu Ala Phe Val 
            20      


<210>  43
<211>  52
<212>  PRT
<213>  Homo sapiens

<400>  43

Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala 
1               5                   10                  15      


Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu 
            20                  25                  30          


Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg 
        35                  40                  45              


Pro Tyr Tyr Lys 
    50          


<210>  44
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tim4 binding domain-CD28 hinge-CD28 transmembrane-CD28 
       costimulatory signaling domain-DAP12 signaling domain


<220>
<221>  SIGNAL
<222>  (1)..(24)

<400>  44

Met Ser Lys Glu Pro Leu Ile Leu Trp Leu Met Ile Glu Phe Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Val Thr Ser Glu Thr Val Val Thr Glu Val Leu 
            20                  25                  30          


Gly His Arg Val Thr Leu Pro Cys Leu Tyr Ser Ser Trp Ser His Asn 
        35                  40                  45              


Ser Asn Ser Met Cys Trp Gly Lys Asp Gln Cys Pro Tyr Ser Gly Cys 
    50                  55                  60                  


Lys Glu Ala Leu Ile Arg Thr Asp Gly Met Arg Val Thr Ser Arg Lys 
65                  70                  75                  80  


Ser Ala Lys Tyr Arg Leu Gln Gly Thr Ile Pro Arg Gly Asp Val Ser 
                85                  90                  95      


Leu Thr Ile Leu Asn Pro Ser Glu Ser Asp Ser Gly Val Tyr Cys Cys 
            100                 105                 110         


Arg Ile Glu Val Pro Gly Trp Phe Asn Asp Val Lys Ile Asn Val Arg 
        115                 120                 125             


Leu Asn Leu Gln Arg Ala Ser Thr Thr Thr His Arg Thr Ala Thr Thr 
    130                 135                 140                 


Thr Thr Arg Arg Thr Thr Thr Thr Ser Pro Thr Thr Thr Arg Gln Met 
145                 150                 155                 160 


Thr Thr Thr Pro Ala Ala Leu Pro Thr Thr Val Val Thr Thr Pro Asp 
                165                 170                 175     


Leu Thr Thr Gly Thr Pro Leu Gln Met Thr Thr Ile Ala Val Phe Thr 
            180                 185                 190         


Thr Ala Asn Thr Cys Leu Ser Leu Thr Pro Ser Thr Leu Pro Glu Glu 
        195                 200                 205             


Ala Thr Gly Leu Leu Thr Pro Glu Pro Ser Lys Glu Gly Pro Ile Leu 
    210                 215                 220                 


Thr Ala Glu Ser Glu Thr Val Leu Pro Ser Asp Ser Trp Ser Ser Val 
225                 230                 235                 240 


Glu Ser Thr Ser Ala Asp Thr Val Leu Leu Thr Ser Lys Glu Ser Lys 
                245                 250                 255     


Val Trp Asp Leu Pro Ser Thr Ser His Val Ser Met Trp Lys Thr Ser 
            260                 265                 270         


Asp Ser Val Ser Ser Pro Gln Pro Gly Ala Ser Asp Thr Ala Val Pro 
        275                 280                 285             


Glu Gln Asn Lys Thr Thr Lys Thr Gly Gln Met Asp Gly Ile Pro Met 
    290                 295                 300                 


Ser Met Lys Asn Glu Met Pro Ile Ser Gln Ile Glu Val Met Tyr Pro 
305                 310                 315                 320 


Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val 
                325                 330                 335     


Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys 
            340                 345                 350         


Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
        355                 360                 365             


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg 
    370                 375                 380                 


Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
385                 390                 395                 400 


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
                405                 410                 415     


Ala Ala Tyr Arg Ser Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg 
            420                 425                 430         


Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu 
        435                 440                 445             


Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp 
    450                 455                 460                 


Leu Asn Thr Gln Arg Pro Tyr Tyr Lys 
465                 470             


<210>  45
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tim4 binding domain-modified IgG4 hinge-CD28 transmembrane-CD28 
       costimulatory signaling domain-DAP12 signaling domain


<220>
<221>  SIGNAL
<222>  (1)..(24)

<400>  45

Met Ser Lys Glu Pro Leu Ile Leu Trp Leu Met Ile Glu Phe Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Val Thr Ser Glu Thr Val Val Thr Glu Val Leu 
            20                  25                  30          


Gly His Arg Val Thr Leu Pro Cys Leu Tyr Ser Ser Trp Ser His Asn 
        35                  40                  45              


Ser Asn Ser Met Cys Trp Gly Lys Asp Gln Cys Pro Tyr Ser Gly Cys 
    50                  55                  60                  


Lys Glu Ala Leu Ile Arg Thr Asp Gly Met Arg Val Thr Ser Arg Lys 
65                  70                  75                  80  


Ser Ala Lys Tyr Arg Leu Gln Gly Thr Ile Pro Arg Gly Asp Val Ser 
                85                  90                  95      


Leu Thr Ile Leu Asn Pro Ser Glu Ser Asp Ser Gly Val Tyr Cys Cys 
            100                 105                 110         


Arg Ile Glu Val Pro Gly Trp Phe Asn Asp Val Lys Ile Asn Val Arg 
        115                 120                 125             


Leu Asn Leu Gln Arg Ala Ser Thr Thr Thr His Arg Thr Ala Thr Thr 
    130                 135                 140                 


Thr Thr Arg Arg Thr Thr Thr Thr Ser Pro Thr Thr Thr Arg Gln Met 
145                 150                 155                 160 


Thr Thr Thr Pro Ala Ala Leu Pro Thr Thr Val Val Thr Thr Pro Asp 
                165                 170                 175     


Leu Thr Thr Gly Thr Pro Leu Gln Met Thr Thr Ile Ala Val Phe Thr 
            180                 185                 190         


Thr Ala Asn Thr Cys Leu Ser Leu Thr Pro Ser Thr Leu Pro Glu Glu 
        195                 200                 205             


Ala Thr Gly Leu Leu Thr Pro Glu Pro Ser Lys Glu Gly Pro Ile Leu 
    210                 215                 220                 


Thr Ala Glu Ser Glu Thr Val Leu Pro Ser Asp Ser Trp Ser Ser Val 
225                 230                 235                 240 


Glu Ser Thr Ser Ala Asp Thr Val Leu Leu Thr Ser Lys Glu Ser Lys 
                245                 250                 255     


Val Trp Asp Leu Pro Ser Thr Ser His Val Ser Met Trp Lys Thr Ser 
            260                 265                 270         


Asp Ser Val Ser Ser Pro Gln Pro Gly Ala Ser Asp Thr Ala Val Pro 
        275                 280                 285             


Glu Gln Asn Lys Thr Thr Lys Thr Gly Gln Met Asp Gly Ile Pro Met 
    290                 295                 300                 


Ser Met Lys Asn Glu Met Pro Ile Ser Gln Glu Ser Lys Tyr Gly Pro 
305                 310                 315                 320 


Pro Cys Pro Pro Cys Pro Phe Trp Val Leu Val Val Val Gly Gly Val 
                325                 330                 335     


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            340                 345                 350         


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
        355                 360                 365             


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
    370                 375                 380                 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Tyr Phe Leu Gly Arg Leu 
385                 390                 395                 400 


Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg 
                405                 410                 415     


Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser 
            420                 425                 430         


Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg Pro Tyr Tyr Lys 
        435                 440                 445     


<210>  46
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  46

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  47
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  47

Met Ser Lys Glu Pro Leu Ile Leu Trp Leu Met Ile Glu Phe Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Val Thr Ser 
            20                  


<210>  48
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A self-cleaving peptide

<400>  48

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  49
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A self-cleaving peptide

<400>  49

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  50
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A self-cleaving peptide

<400>  50

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  51
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A self-cleaving peptide

<400>  51

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  52
<211>  285
<212>  PRT
<213>  Homo sapiens

<400>  52

Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         


Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His 
        275                 280                 285 


<210>  53
<211>  192
<212>  PRT
<213>  Homo sapiens

<400>  53

Met Gln Ala Ile Lys Cys Val Val Val Gly Asp Gly Ala Val Gly Lys 
1               5                   10                  15      


Thr Cys Leu Leu Ile Ser Tyr Thr Thr Asn Ala Phe Pro Gly Glu Tyr 
            20                  25                  30          


Ile Pro Thr Val Phe Asp Asn Tyr Ser Ala Asn Val Met Val Asp Gly 
        35                  40                  45              


Lys Pro Val Asn Leu Gly Leu Trp Asp Thr Ala Gly Gln Glu Asp Tyr 
    50                  55                  60                  


Asp Arg Leu Arg Pro Leu Ser Tyr Pro Gln Thr Asp Val Phe Leu Ile 
65                  70                  75                  80  


Cys Phe Ser Leu Val Ser Pro Ala Ser Phe Glu Asn Val Arg Ala Lys 
                85                  90                  95      


Trp Tyr Pro Glu Val Arg His His Cys Pro Asn Thr Pro Ile Ile Leu 
            100                 105                 110         


Val Gly Thr Lys Leu Asp Leu Arg Asp Asp Lys Asp Thr Ile Glu Lys 
        115                 120                 125             


Leu Lys Glu Lys Lys Leu Thr Pro Ile Thr Tyr Pro Gln Gly Leu Ala 
    130                 135                 140                 


Met Ala Lys Glu Ile Gly Ala Val Lys Tyr Leu Glu Cys Ser Ala Leu 
145                 150                 155                 160 


Thr Gln Arg Gly Leu Lys Thr Val Phe Asp Glu Ala Ile Arg Ala Val 
                165                 170                 175     


Leu Cys Pro Pro Pro Val Lys Lys Arg Lys Arg Lys Cys Leu Leu Leu 
            180                 185                 190         


<210>  54
<211>  215
<212>  PRT
<213>  Homo sapiens

<400>  54

Met Ala Ser Arg Gly Ala Thr Arg Pro Asn Gly Pro Asn Thr Gly Asn 
1               5                   10                  15      


Lys Ile Cys Gln Phe Lys Leu Val Leu Leu Gly Glu Ser Ala Val Gly 
            20                  25                  30          


Lys Ser Ser Leu Val Leu Arg Phe Val Lys Gly Gln Phe His Glu Phe 
        35                  40                  45              


Gln Glu Ser Thr Ile Gly Ala Ala Phe Leu Thr Gln Thr Val Cys Leu 
    50                  55                  60                  


Asp Asp Thr Thr Val Lys Phe Glu Ile Trp Asp Thr Ala Gly Gln Glu 
65                  70                  75                  80  


Arg Tyr His Ser Leu Ala Pro Met Tyr Tyr Arg Gly Ala Gln Ala Ala 
                85                  90                  95      


Ile Val Val Tyr Asp Ile Thr Asn Glu Glu Ser Phe Ala Arg Ala Lys 
            100                 105                 110         


Asn Trp Val Lys Glu Leu Gln Arg Gln Ala Ser Pro Asn Ile Val Ile 
        115                 120                 125             


Ala Leu Ser Gly Asn Lys Ala Asp Leu Ala Asn Lys Arg Ala Val Asp 
    130                 135                 140                 


Phe Gln Glu Ala Gln Ser Tyr Ala Asp Asp Asn Ser Leu Leu Phe Met 
145                 150                 155                 160 


Glu Thr Ser Ala Lys Thr Ser Met Asn Val Asn Glu Ile Phe Met Ala 
                165                 170                 175     


Ile Ala Lys Lys Leu Pro Lys Asn Glu Pro Gln Asn Pro Gly Ala Asn 
            180                 185                 190         


Ser Ala Arg Gly Arg Gly Val Asp Leu Thr Glu Pro Thr Gln Pro Thr 
        195                 200                 205             


Arg Asn Gln Cys Cys Ser Asn 
    210                 215 


<210>  55
<211>  207
<212>  PRT
<213>  Homo sapiens

<400>  55

Met Thr Ser Arg Lys Lys Val Leu Leu Lys Val Ile Ile Leu Gly Asp 
1               5                   10                  15      


Ser Gly Val Gly Lys Thr Ser Leu Met Asn Gln Tyr Val Asn Lys Lys 
            20                  25                  30          


Phe Ser Asn Gln Tyr Lys Ala Thr Ile Gly Ala Asp Phe Leu Thr Lys 
        35                  40                  45              


Glu Val Met Val Asp Asp Arg Leu Val Thr Met Gln Ile Trp Asp Thr 
    50                  55                  60                  


Ala Gly Gln Glu Arg Phe Gln Ser Leu Gly Val Ala Phe Tyr Arg Gly 
65                  70                  75                  80  


Ala Asp Cys Cys Val Leu Val Phe Asp Val Thr Ala Pro Asn Thr Phe 
                85                  90                  95      


Lys Thr Leu Asp Ser Trp Arg Asp Glu Phe Leu Ile Gln Ala Ser Pro 
            100                 105                 110         


Arg Asp Pro Glu Asn Phe Pro Phe Val Val Leu Gly Asn Lys Ile Asp 
        115                 120                 125             


Leu Glu Asn Arg Gln Val Ala Thr Lys Arg Ala Gln Ala Trp Cys Tyr 
    130                 135                 140                 


Ser Lys Asn Asn Ile Pro Tyr Phe Glu Thr Ser Ala Lys Glu Ala Ile 
145                 150                 155                 160 


Asn Val Glu Gln Ala Phe Gln Thr Ile Ala Arg Asn Ala Leu Lys Gln 
                165                 170                 175     


Glu Thr Glu Val Glu Leu Tyr Asn Glu Phe Pro Glu Pro Ile Lys Leu 
            180                 185                 190         


Asp Lys Asn Asp Arg Ala Lys Ala Ser Ala Glu Ser Cys Ser Cys 
        195                 200                 205         


<210>  56
<211>  184
<212>  PRT
<213>  Homo sapiens

<400>  56

Met Arg Glu Tyr Lys Leu Val Val Leu Gly Ser Gly Gly Val Gly Lys 
1               5                   10                  15      


Ser Ala Leu Thr Val Gln Phe Val Gln Gly Ile Phe Val Glu Lys Tyr 
            20                  25                  30          


Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Glu Val Asp Cys 
        35                  40                  45              


Gln Gln Cys Met Leu Glu Ile Leu Asp Thr Ala Gly Thr Glu Gln Phe 
    50                  55                  60                  


Thr Ala Met Arg Asp Leu Tyr Met Lys Asn Gly Gln Gly Phe Ala Leu 
65                  70                  75                  80  


Val Tyr Ser Ile Thr Ala Gln Ser Thr Phe Asn Asp Leu Gln Asp Leu 
                85                  90                  95      


Arg Glu Gln Ile Leu Arg Val Lys Asp Thr Glu Asp Val Pro Met Ile 
            100                 105                 110         


Leu Val Gly Asn Lys Cys Asp Leu Glu Asp Glu Arg Val Val Gly Lys 
        115                 120                 125             


Glu Gln Gly Gln Asn Leu Ala Arg Gln Trp Cys Asn Cys Ala Phe Leu 
    130                 135                 140                 


Glu Ser Ser Ala Lys Ser Lys Ile Asn Val Asn Glu Ile Phe Tyr Asp 
145                 150                 155                 160 


Leu Val Arg Gln Ile Asn Arg Lys Thr Pro Val Glu Lys Lys Lys Pro 
                165                 170                 175     


Lys Lys Lys Ser Cys Leu Leu Leu 
            180                 


<210>  57
<211>  193
<212>  PRT
<213>  Homo sapiens

<400>  57

Met Ala Ala Ile Arg Lys Lys Leu Val Ile Val Gly Asp Gly Ala Cys 
1               5                   10                  15      


Gly Lys Thr Cys Leu Leu Ile Val Phe Ser Lys Asp Gln Phe Pro Glu 
            20                  25                  30          


Val Tyr Val Pro Thr Val Phe Glu Asn Tyr Val Ala Asp Ile Glu Val 
        35                  40                  45              


Asp Gly Lys Gln Val Glu Leu Ala Leu Trp Asp Thr Ala Gly Gln Glu 
    50                  55                  60                  


Asp Tyr Asp Arg Leu Arg Pro Leu Ser Tyr Pro Asp Thr Asp Val Ile 
65                  70                  75                  80  


Leu Met Cys Phe Ser Ile Asp Ser Pro Asp Ser Leu Glu Asn Ile Pro 
                85                  90                  95      


Glu Lys Trp Thr Pro Glu Val Lys His Phe Cys Pro Asn Val Pro Ile 
            100                 105                 110         


Ile Leu Val Gly Asn Lys Lys Asp Leu Arg Asn Asp Glu His Thr Arg 
        115                 120                 125             


Arg Glu Leu Ala Lys Met Lys Gln Glu Pro Val Lys Pro Glu Glu Gly 
    130                 135                 140                 


Arg Asp Met Ala Asn Arg Ile Gly Ala Phe Gly Tyr Met Glu Cys Ser 
145                 150                 155                 160 


Ala Lys Thr Lys Asp Gly Val Arg Glu Val Phe Glu Met Ala Thr Arg 
                165                 170                 175     


Ala Ala Leu Gln Ala Arg Arg Gly Lys Lys Lys Ser Gly Cys Leu Val 
            180                 185                 190         


Leu 
    


<210>  58
<211>  191
<212>  PRT
<213>  Homo sapiens

<400>  58

Met Gln Thr Ile Lys Cys Val Val Val Gly Asp Gly Ala Val Gly Lys 
1               5                   10                  15      


Thr Cys Leu Leu Ile Ser Tyr Thr Thr Asn Lys Phe Pro Ser Glu Tyr 
            20                  25                  30          


Val Pro Thr Val Phe Asp Asn Tyr Ala Val Thr Val Met Ile Gly Gly 
        35                  40                  45              


Glu Pro Tyr Thr Leu Gly Leu Phe Asp Thr Ala Gly Gln Glu Asp Tyr 
    50                  55                  60                  


Asp Arg Leu Arg Pro Leu Ser Tyr Pro Gln Thr Asp Val Phe Leu Val 
65                  70                  75                  80  


Cys Phe Ser Val Val Ser Pro Ser Ser Phe Glu Asn Val Lys Glu Lys 
                85                  90                  95      


Trp Val Pro Glu Ile Thr His His Cys Pro Lys Thr Pro Phe Leu Leu 
            100                 105                 110         


Val Gly Thr Gln Ile Asp Leu Arg Asp Asp Pro Ser Thr Ile Glu Lys 
        115                 120                 125             


Leu Ala Lys Asn Lys Gln Lys Pro Ile Thr Pro Glu Thr Ala Glu Lys 
    130                 135                 140                 


Leu Ala Arg Asp Leu Lys Ala Val Lys Tyr Val Glu Cys Ser Ala Leu 
145                 150                 155                 160 


Thr Gln Lys Gly Leu Lys Asn Val Phe Asp Glu Ala Ile Leu Ala Ala 
                165                 170                 175     


Leu Glu Pro Pro Glu Pro Lys Lys Ser Arg Arg Cys Val Leu Leu 
            180                 185                 190     


<210>  59
<211>  21
<212>  PRT
<213>  Mus musculus

<400>  59

Ile Leu Ile Ile Ala Cys Cys Val Gly Phe Val Leu Met Val Leu Leu 
1               5                   10                  15      


Phe Leu Ala Phe Leu 
            20      


<210>  60
<211>  279
<212>  PRT
<213>  Mus musculus


<220>
<221>  SIGNAL
<222>  (1)..(22)

<400>  60

Met Ser Lys Gly Leu Leu Leu Leu Trp Leu Val Thr Glu Leu Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Ala Ala Ser Glu Asp Thr Ile Ile Gly Phe Leu 
            20                  25                  30          


Gly Gln Pro Val Thr Leu Pro Cys His Tyr Leu Ser Trp Ser Gln Ser 
        35                  40                  45              


Arg Asn Ser Met Cys Trp Gly Lys Gly Ser Cys Pro Asn Ser Lys Cys 
    50                  55                  60                  


Asn Ala Glu Leu Leu Arg Thr Asp Gly Thr Arg Ile Ile Ser Arg Lys 
65                  70                  75                  80  


Ser Thr Lys Tyr Thr Leu Leu Gly Lys Val Gln Phe Gly Glu Val Ser 
                85                  90                  95      


Leu Thr Ile Ser Asn Thr Asn Arg Gly Asp Ser Gly Val Tyr Cys Cys 
            100                 105                 110         


Arg Ile Glu Val Pro Gly Trp Phe Asn Asp Val Lys Lys Asn Val Arg 
        115                 120                 125             


Leu Glu Leu Arg Arg Ala Thr Thr Thr Lys Lys Pro Thr Thr Thr Thr 
    130                 135                 140                 


Arg Pro Thr Thr Thr Pro Tyr Val Thr Thr Thr Thr Pro Glu Leu Leu 
145                 150                 155                 160 


Pro Thr Thr Val Met Thr Thr Ser Val Leu Pro Thr Thr Thr Pro Pro 
                165                 170                 175     


Gln Thr Leu Ala Thr Thr Ala Phe Ser Thr Ala Val Thr Thr Cys Pro 
            180                 185                 190         


Ser Thr Thr Pro Gly Ser Phe Ser Gln Glu Thr Thr Lys Gly Ser Ala 
        195                 200                 205             


Phe Thr Thr Glu Ser Glu Thr Leu Pro Ala Ser Asn His Ser Gln Arg 
    210                 215                 220                 


Ser Met Met Thr Ile Ser Thr Asp Ile Ala Val Leu Arg Pro Thr Gly 
225                 230                 235                 240 


Ser Asn Pro Gly Ile Leu Pro Ser Thr Ser Gln Leu Thr Thr Gln Lys 
                245                 250                 255     


Thr Thr Leu Thr Thr Ser Glu Ser Leu Gln Lys Thr Thr Lys Ser His 
            260                 265                 270         


Gln Ile Asn Ser Arg Gln Thr 
        275                 


<210>  61
<211>  22
<212>  PRT
<213>  Mus musculus

<400>  61

Met Ser Lys Gly Leu Leu Leu Leu Trp Leu Val Thr Glu Leu Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Ala 
            20          


<210>  62
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human CD28 costimulatory signaling domain with L186G/L187G 
       substitutions (positions reference to full length protein)

<400>  62

Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  63
<211>  113
<212>  PRT
<213>  Homo sapiens

<400>  63

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Arg 
    


<210>  64
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A self-cleaving peptide variant

<400>  64

Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      


Val Glu Glu Asn Pro Gly Pro 
            20              


<210>  65
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A self-cleaving peptide variant

<400>  65

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro Arg 
            


<210>  66
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A self-cleaving peptide variant

<400>  66

Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      


Val Glu Glu Asn Pro Gly Pro Arg 
            20                  


<210>  67
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A self-cleaving peptide variant

<400>  67

Arg Ala Lys Arg Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys 
1               5                   10                  15      


Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            20                  25          


<210>  68
<211>  434
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tim4 binding domain-CD28 transmembrane-CD28 costimulatory 
       signaling domain-DAP12 signaling domain


<220>
<221>  SIGNAL
<222>  (1)..(24)

<400>  68

Met Ser Lys Glu Pro Leu Ile Leu Trp Leu Met Ile Glu Phe Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Val Thr Ser Glu Thr Val Val Thr Glu Val Leu 
            20                  25                  30          


Gly His Arg Val Thr Leu Pro Cys Leu Tyr Ser Ser Trp Ser His Asn 
        35                  40                  45              


Ser Asn Ser Met Cys Trp Gly Lys Asp Gln Cys Pro Tyr Ser Gly Cys 
    50                  55                  60                  


Lys Glu Ala Leu Ile Arg Thr Asp Gly Met Arg Val Thr Ser Arg Lys 
65                  70                  75                  80  


Ser Ala Lys Tyr Arg Leu Gln Gly Thr Ile Pro Arg Gly Asp Val Ser 
                85                  90                  95      


Leu Thr Ile Leu Asn Pro Ser Glu Ser Asp Ser Gly Val Tyr Cys Cys 
            100                 105                 110         


Arg Ile Glu Val Pro Gly Trp Phe Asn Asp Val Lys Ile Asn Val Arg 
        115                 120                 125             


Leu Asn Leu Gln Arg Ala Ser Thr Thr Thr His Arg Thr Ala Thr Thr 
    130                 135                 140                 


Thr Thr Arg Arg Thr Thr Thr Thr Ser Pro Thr Thr Thr Arg Gln Met 
145                 150                 155                 160 


Thr Thr Thr Pro Ala Ala Leu Pro Thr Thr Val Val Thr Thr Pro Asp 
                165                 170                 175     


Leu Thr Thr Gly Thr Pro Leu Gln Met Thr Thr Ile Ala Val Phe Thr 
            180                 185                 190         


Thr Ala Asn Thr Cys Leu Ser Leu Thr Pro Ser Thr Leu Pro Glu Glu 
        195                 200                 205             


Ala Thr Gly Leu Leu Thr Pro Glu Pro Ser Lys Glu Gly Pro Ile Leu 
    210                 215                 220                 


Thr Ala Glu Ser Glu Thr Val Leu Pro Ser Asp Ser Trp Ser Ser Val 
225                 230                 235                 240 


Glu Ser Thr Ser Ala Asp Thr Val Leu Leu Thr Ser Lys Glu Ser Lys 
                245                 250                 255     


Val Trp Asp Leu Pro Ser Thr Ser His Val Ser Met Trp Lys Thr Ser 
            260                 265                 270         


Asp Ser Val Ser Ser Pro Gln Pro Gly Ala Ser Asp Thr Ala Val Pro 
        275                 280                 285             


Glu Gln Asn Lys Thr Thr Lys Thr Gly Gln Met Asp Gly Ile Pro Met 
    290                 295                 300                 


Ser Met Lys Asn Glu Met Pro Ile Ser Gln Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Tyr Phe 
    370                 375                 380                 


Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr 
385                 390                 395                 400 


Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln 
                405                 410                 415     


Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg Pro Tyr 
            420                 425                 430         


Tyr Lys 
        


<210>  69
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 HCDR1

<400>  69

Gly Tyr Leu Met Ser 
1               5   


<210>  70
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 HCDR2

<400>  70

Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  71
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 HCDR3

<400>  71

Ala Arg Arg Asp Thr Asn Leu Phe Asp Tyr 
1               5                   10  


<210>  72
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 LCDR1

<400>  72

Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn 
1               5                   10      


<210>  73
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 LCDR2

<400>  73

Ala Ala Ser Ser Leu Gln Ser 
1               5           


<210>  74
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 LCDR3

<400>  74

Gln Gln Ser Tyr Ser Thr Pro Pro Thr 
1               5                   


<210>  75
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 HCDR1

<400>  75

Ser Tyr Tyr Met His 
1               5   


<210>  76
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 HCDR2

<400>  76

Ile Ile Asn Pro Ser Gly Gly Gly Thr Ser Tyr Ala Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  77
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 HCDR3

<400>  77

Asp Tyr Tyr Val Thr Tyr Asp Ser Trp Phe Asp Ser 
1               5                   10          


<210>  78
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 LCDR1

<400>  78

Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser 
1               5                   10      


<210>  79
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 LCDR2

<400>  79

Gly Lys Asn Asn Arg Pro Ser 
1               5           


<210>  80
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 LCDR3

<400>  80

Asn Ser Arg Asp Ser Ser Gly Asn His Val Val 
1               5                   10      


<210>  81
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 scFv

<400>  81

Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Gly 
            20                  25                  30          


Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly Gly Ser Gly Gly Thr 
        115                 120                 125             


Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln Ser Pro Ser Ser Leu 
    130                 135                 140                 


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
145                 150                 155                 160 


Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
                165                 170                 175     


Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
        195                 200                 205             


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser 
    210                 215                 220                 


Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
225                 230                 235                 240 


Arg Ala Ala Ala 
                


<210>  82
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 scFv

<400>  82

Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser 
            20                  25                  30          


Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr Ser Tyr Ala Gln Lys 
    50                  55                  60                  


Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val 
65                  70                  75                  80  


Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser Trp Phe Asp Ser Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu Leu Thr Gln Asp Pro 
    130                 135                 140                 


Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg Ile Thr Cys Gln Gly 
145                 150                 155                 160 


Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     


Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn Asn Arg Pro Ser Gly 
            180                 185                 190         


Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Asn Thr Ala Ser Leu 
        195                 200                 205             


Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Asn 
    210                 215                 220                 


Ser Arg Asp Ser Ser Gly Asn His Val Val Phe Gly Gly Gly Thr Lys 
225                 230                 235                 240 


Leu Thr Val Leu Gly Ala Ala Ala 
                245             


<210>  83
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  flexible linker

<400>  83

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  84
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  84

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  85
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  85

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro 
        35                  


<210>  86
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  86

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  87
<211>  458
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pCTX206 CAR-GMSCFR signal peptide-sc02-004 scFv-IgG4 Hinge-CD28 TM-CD28 ICD-CD3z

<400>  87

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Glu Ser Lys Tyr Gly Pro Pro 
            260                 265                 270         


Cys Pro Pro Cys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
        275                 280                 285             


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
    290                 295                 300                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
305                 310                 315                 320 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                325                 330                 335     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
            340                 345                 350         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        355                 360                 365             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    370                 375                 380                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455             


<210>  88
<211>  462
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pCTX208 CAR-GMSCFR signal peptide-sc02-025 scFv-IgG4 Hinge-CD28 TM-CD28 ICD-CD3z

<400>  88

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Glu Ser Lys 
            260                 265                 270         


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Phe Trp Val Leu Val Val Val 
        275                 280                 285             


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
    290                 295                 300                 


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
305                 310                 315                 320 


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
                325                 330                 335     


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
            340                 345                 350         


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
        355                 360                 365             


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
    370                 375                 380                 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg 
385                 390                 395                 400 


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
                405                 410                 415     


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
            420                 425                 430         


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
        435                 440                 445             


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460         


<210>  89
<211>  459
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-004 scFv-IgG4 Hinge-CD28TM-4-1BB ICD-CD3z

<400>  89

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Glu Ser Lys Tyr Gly Pro Pro 
            260                 265                 270         


Cys Pro Pro Cys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
        275                 280                 285             


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
    290                 295                 300                 


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
305                 310                 315                 320 


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
                325                 330                 335     


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn 
385                 390                 395                 400 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
                405                 410                 415     


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
            420                 425                 430         


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
        435                 440                 445             


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 


<210>  90
<211>  463
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-025 scFv-IgG4 Hinge-CD28TM-4-1BB ICD-CD3z

<400>  90

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Glu Ser Lys 
            260                 265                 270         


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Phe Trp Val Leu Val Val Val 
        275                 280                 285             


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
    290                 295                 300                 


Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys 
305                 310                 315                 320 


Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys 
                325                 330                 335     


Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val 
            340                 345                 350         


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
        355                 360                 365             


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
    370                 375                 380                 


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln 
385                 390                 395                 400 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                405                 410                 415     


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            420                 425                 430         


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        435                 440                 445             


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460             


<210>  91
<211>  485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-004 scFv-CD28 Hinge-CD28TM-CD28 ICD-CD3z

<400>  91

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Ile Glu Val Met Tyr Pro Pro 
            260                 265                 270         


Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys 
        275                 280                 285             


Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro 
    290                 295                 300                 


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
305                 310                 315                 320 


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
                325                 330                 335     


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
            340                 345                 350         


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
        355                 360                 365             


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
    370                 375                 380                 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
385                 390                 395                 400 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
                405                 410                 415     


Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
            420                 425                 430         


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
        435                 440                 445             


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
    450                 455                 460                 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
465                 470                 475                 480 


Ala Leu Pro Pro Arg 
                485 


<210>  92
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-025 scFv-CD28 Hinge-CD28TM-CD28 ICD-CD3z

<400>  92

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Ile Glu Val 
            260                 265                 270         


Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile 
        275                 280                 285             


Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly 
    290                 295                 300                 


Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
305                 310                 315                 320 


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
                325                 330                 335     


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
            340                 345                 350         


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
        355                 360                 365             


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln 
            420                 425                 430         


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        435                 440                 445             


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    450                 455                 460                 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
465                 470                 475                 480 


Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 


<210>  93
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-004 scFv-CD28 Hinge-CD28TM-4-1BB ICD-CD3z

<400>  93

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Ile Glu Val Met Tyr Pro Pro 
            260                 265                 270         


Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys 
        275                 280                 285             


Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro 
    290                 295                 300                 


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
305                 310                 315                 320 


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys 
                325                 330                 335     


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
            340                 345                 350         


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
        355                 360                 365             


Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
    370                 375                 380                 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
385                 390                 395                 400 


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
                405                 410                 415     


Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210>  94
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-025 scFv-CD28 Hinge-CD28TM-4-1BB ICD-CD3z

<400>  94

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Ile Glu Val 
            260                 265                 270         


Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile 
        275                 280                 285             


Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly 
    290                 295                 300                 


Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
305                 310                 315                 320 


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys 
                325                 330                 335     


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
            340                 345                 350         


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
        355                 360                 365             


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
    370                 375                 380                 


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
385                 390                 395                 400 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
                405                 410                 415     


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro 
            420                 425                 430         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490 


<210>  95
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-004 scFv-IgG4 Hinge-CD8aTM-CD28 ICD-CD3z

<400>  95

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Glu Ser Lys Tyr Gly Pro Pro 
            260                 265                 270         


Cys Pro Pro Cys Pro Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
        275                 280                 285             


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg Ser Lys 
    290                 295                 300                 


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
305                 310                 315                 320 


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
                325                 330                 335     


Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
            340                 345                 350         


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
        355                 360                 365             


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
    370                 375                 380                 


Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                405                 410                 415     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            420                 425                 430         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        435                 440                 445             


Met Gln Ala Leu Pro Pro Arg 
    450                 455 


<210>  96
<211>  459
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-025 scFv-IgG4 Hinge-CD8aTM-CD28 ICD-CD3z

<400>  96

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Glu Ser Lys 
            260                 265                 270         


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ile Tyr Ile Trp Ala Pro Leu 
        275                 280                 285             


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
    290                 295                 300                 


Cys Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
305                 310                 315                 320 


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
                325                 330                 335     


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn 
385                 390                 395                 400 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
                405                 410                 415     


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
            420                 425                 430         


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
        435                 440                 445             


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 


<210>  97
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-004 scFv-CD8a Hinge-CD8aTM-CD28 ICD-CD3z

<400>  97

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg Ser 
                325                 330                 335     


Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
            340                 345                 350         


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
        355                 360                 365             


Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
    370                 375                 380                 


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
385                 390                 395                 400 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
                405                 410                 415     


Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg 
                485             


<210>  98
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-025 scFv-CD8a Hinge-CD8aTM-CD28 ICD-CD3z

<400>  98

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
305                 310                 315                 320 


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
                325                 330                 335     


Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
            340                 345                 350         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        355                 360                 365             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser 
    370                 375                 380                 


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
385                 390                 395                 400 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                405                 410                 415     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
            420                 425                 430         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490         


<210>  99
<211>  456
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-004 scFv-IgG4 Hinge-CD8aTM-4-1BB ICD-CD3z

<400>  99

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Glu Ser Lys Tyr Gly Pro Pro 
            260                 265                 270         


Cys Pro Pro Cys Pro Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
        275                 280                 285             


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly 
    290                 295                 300                 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
305                 310                 315                 320 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
                325                 330                 335     


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
            340                 345                 350         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        355                 360                 365             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    370                 375                 380                 


Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu 
385                 390                 395                 400 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                405                 410                 415     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            420                 425                 430         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        435                 440                 445             


His Met Gln Ala Leu Pro Pro Arg 
    450                 455     


<210>  100
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-025 scFv-IgG4 Hinge-CD8aTM-4-1BB ICD-CD3z

<400>  100

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Glu Ser Lys 
            260                 265                 270         


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ile Tyr Ile Trp Ala Pro Leu 
        275                 280                 285             


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
    290                 295                 300                 


Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
305                 310                 315                 320 


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
                325                 330                 335     


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
            340                 345                 350         


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
        355                 360                 365             


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
    370                 375                 380                 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
385                 390                 395                 400 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                405                 410                 415     


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            420                 425                 430         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        435                 440                 445             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460 


<210>  101
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-004 scFv-CD8a Hinge-CD8aTM-4-1BB ICD-CD3z

<400>  101

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Ser Gly Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys 
65                  70                  75                  80  


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly 
    130                 135                 140                 


Gly Ser Gly Gly Thr Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
                165                 170                 175     


Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu 
        195                 200                 205             


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            340                 345                 350         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        355                 360                 365             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln 
            420                 425                 430         


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        435                 440                 445             


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    450                 455                 460                 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
465                 470                 475                 480 


Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 


<210>  102
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-GMSCFR signal peptide-sc02-025 scFv-CD8a Hinge-CD8a TM-4-1BB ICD-CD3z

<400>  102

Met Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr 
65                  70                  75                  80  


Ser Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser 
        115                 120                 125             


Trp Phe Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu 
145                 150                 155                 160 


Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg 
                165                 170                 175     


Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr 
            180                 185                 190         


Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn 
        195                 200                 205             


Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly 
    210                 215                 220                 


Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala 
225                 230                 235                 240 


Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His Val Val Phe 
                245                 250                 255     


Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Ala Ala Ala Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
305                 310                 315                 320 


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
                325                 330                 335     


Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
            340                 345                 350         


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
        355                 360                 365             


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
    370                 375                 380                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
385                 390                 395                 400 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                405                 410                 415     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490             


<210>  103
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 VH domain

<400>  103

Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
1               5                   10                  15      


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Gly Tyr Leu Met Ser 
            20                  25                  30          


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val Ile 
        35                  40                  45              


Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg 
    50                  55                  60                  


Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met 
65                  70                  75                  80  


Asp Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ala 
                85                  90                  95      


Arg Arg Asp Thr Asn Leu Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val 
        


<210>  104
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 VL domain

<400>  104

Glu Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
1               5                   10                  15      


Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn 
            20                  25                  30          


Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala 
        35                  40                  45              


Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    50                  55                  60                  


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
65                  70                  75                  80  


Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Pro Thr Phe 
                85                  90                  95      


Gly Gln Gly Thr Lys Val Glu Ile 
            100                 


<210>  105
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 VH domain

<400>  105

Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys 
1               5                   10                  15      


Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Met His 
            20                  25                  30          


Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Ile Ile 
        35                  40                  45              


Asn Pro Ser Gly Gly Gly Thr Ser Tyr Ala Gln Lys Phe Gln Gly Arg 
    50                  55                  60                  


Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr Met Glu Leu 
65                  70                  75                  80  


Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp 
                85                  90                  95      


Tyr Tyr Val Thr Tyr Asp Ser Trp Phe Asp Ser Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser 
        115         


<210>  106
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 VL domain

<400>  106

Val Ser Val Ala Leu Gly Gln Thr Val Arg Ile Thr Cys Gln Gly Asp 
1               5                   10                  15      


Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr Gln Gln Lys Pro Gly Gln 
            20                  25                  30          


Ala Pro Val Leu Val Ile Tyr Gly Lys Asn Asn Arg Pro Ser Gly Ile 
        35                  40                  45              


Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Asn Thr Ala Ser Leu Thr 
    50                  55                  60                  


Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Asn Ser 
65                  70                  75                  80  


Arg Asp Ser Ser Gly Asn His Val Val Phe Gly Gly Gly Thr Lys Leu 
                85                  90                  95      


Thr Val 
        


<210>  107
<211>  428
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tim4 binding domain-Tim4 transmembrane domain-CD28 costimulatory signaling domain-DAP12 signaling domain


<220>
<221>  SIGNAL
<222>  (1)..(24)

<400>  107

Met Ser Lys Glu Pro Leu Ile Leu Trp Leu Met Ile Glu Phe Trp Trp 
1               5                   10                  15      


Leu Tyr Leu Thr Pro Val Thr Ser Glu Thr Val Val Thr Glu Val Leu 
            20                  25                  30          


Gly His Arg Val Thr Leu Pro Cys Leu Tyr Ser Ser Trp Ser His Asn 
        35                  40                  45              


Ser Asn Ser Met Cys Trp Gly Lys Asp Gln Cys Pro Tyr Ser Gly Cys 
    50                  55                  60                  


Lys Glu Ala Leu Ile Arg Thr Asp Gly Met Arg Val Thr Ser Arg Lys 
65                  70                  75                  80  


Ser Ala Lys Tyr Arg Leu Gln Gly Thr Ile Pro Arg Gly Asp Val Ser 
                85                  90                  95      


Leu Thr Ile Leu Asn Pro Ser Glu Ser Asp Ser Gly Val Tyr Cys Cys 
            100                 105                 110         


Arg Ile Glu Val Pro Gly Trp Phe Asn Asp Val Lys Ile Asn Val Arg 
        115                 120                 125             


Leu Asn Leu Gln Arg Ala Ser Thr Thr Thr His Arg Thr Ala Thr Thr 
    130                 135                 140                 


Thr Thr Arg Arg Thr Thr Thr Thr Ser Pro Thr Thr Thr Arg Gln Met 
145                 150                 155                 160 


Thr Thr Thr Pro Ala Ala Leu Pro Thr Thr Val Val Thr Thr Pro Asp 
                165                 170                 175     


Leu Thr Thr Gly Thr Pro Leu Gln Met Thr Thr Ile Ala Val Phe Thr 
            180                 185                 190         


Thr Ala Asn Thr Cys Leu Ser Leu Thr Pro Ser Thr Leu Pro Glu Glu 
        195                 200                 205             


Ala Thr Gly Leu Leu Thr Pro Glu Pro Ser Lys Glu Gly Pro Ile Leu 
    210                 215                 220                 


Thr Ala Glu Ser Glu Thr Val Leu Pro Ser Asp Ser Trp Ser Ser Val 
225                 230                 235                 240 


Glu Ser Thr Ser Ala Asp Thr Val Leu Leu Thr Ser Lys Glu Ser Lys 
                245                 250                 255     


Val Trp Asp Leu Pro Ser Thr Ser His Val Ser Met Trp Lys Thr Ser 
            260                 265                 270         


Asp Ser Val Ser Ser Pro Gln Pro Gly Ala Ser Asp Thr Ala Val Pro 
        275                 280                 285             


Glu Gln Asn Lys Thr Thr Lys Thr Gly Gln Met Asp Gly Ile Pro Met 
    290                 295                 300                 


Ser Met Lys Asn Glu Met Pro Ile Ser Gln Leu Leu Met Ile Ile Ala 
305                 310                 315                 320 


Pro Ser Leu Gly Phe Val Leu Phe Ala Leu Phe Val Ala Phe Leu Arg 
                325                 330                 335     


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
            340                 345                 350         


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
        355                 360                 365             


Arg Asp Phe Ala Ala Tyr Arg Ser Tyr Phe Leu Gly Arg Leu Val Pro 
    370                 375                 380                 


Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr 
385                 390                 395                 400 


Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val 
                405                 410                 415     


Tyr Ser Asp Leu Asn Thr Gln Arg Pro Tyr Tyr Lys 
            420                 425             


<210>  108
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-004 scFv

<400>  108

Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Gly 
            20                  25                  30          


Tyr Leu Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Val Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asp Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Ala Arg Arg Asp Thr Asn Leu Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Leu Glu Gly Thr Gly Gly Ser Gly Gly Thr 
        115                 120                 125             


Gly Ser Gly Thr Gly Thr Ser Glu Leu Thr Gln Ser Pro Ser Ser Leu 
    130                 135                 140                 


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
145                 150                 155                 160 


Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
                165                 170                 175     


Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro 
            180                 185                 190         


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
        195                 200                 205             


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser 
    210                 215                 220                 


Tyr Ser Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
225                 230                 235                 240 


Arg Ala Ala Ala 
                


<210>  109
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc02-025 scFv

<400>  109

Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser 
            20                  25                  30          


Tyr Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Ile Ile Asn Pro Ser Gly Gly Gly Thr Ser Tyr Ala Gln Lys 
    50                  55                  60                  


Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val 
65                  70                  75                  80  


Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Tyr Tyr Val Thr Tyr Asp Ser Trp Phe Asp Ser Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Arg Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Glu Leu Thr Gln Asp Pro 
    130                 135                 140                 


Ala Val Ser Val Ala Leu Gly Gln Thr Val Arg Ile Thr Cys Gln Gly 
145                 150                 155                 160 


Asp Ser Leu Arg Ser Tyr Tyr Ala Ser Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     


Gln Ala Pro Val Leu Val Ile Tyr Gly Lys Asn Asn Arg Pro Ser Gly 
            180                 185                 190         


Ile Pro Asp Arg Phe Ser Gly Ser Ser Ser Gly Asn Thr Ala Ser Leu 
        195                 200                 205             


Thr Ile Thr Gly Ala Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Asn 
    210                 215                 220                 


Ser Arg Asp Ser Ser Gly Asn His Val Val Phe Gly Gly Gly Thr Lys 
225                 230                 235                 240 


Leu Thr Val Leu Gly Ala Ala Ala 
                245             


