                         SEQUENCE LISTING

<110>  University of Troms
 
<120>  Method and Kit for Assembly of Multiple DNA Fragments at Room temperature

<130>  P26589PC00

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  611
<212>  PRT
<213>  marine polymerase


<220>
<221>  misc_feature
<222>  (449)..(451)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (521)..(521)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Xaa Xaa Xaa Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Xaa Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  2
<211>  611
<212>  PRT
<213>  marine polymerase

<400>  2

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Ser Ala Phe Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Arg Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  3
<211>  611
<212>  PRT
<213>  marine polymerase modified by mutagenesis

<400>  3

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Ser Asp Phe Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Arg Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  4
<211>  611
<212>  PRT
<213>  marine polymerase modified by mutagenesis

<400>  4

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Ala Ala Ala Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Arg Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  5
<211>  611
<212>  PRT
<213>  marine polymerase modified by mutagenesis

<400>  5

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Ala Asp Phe Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Arg Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  6
<211>  611
<212>  PRT
<213>  marine polymerase modified by mutagenesis

<400>  6

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Ser Asp Ala Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Arg Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  7
<211>  611
<212>  PRT
<213>  marine polymerase modified by mutagenesis

<400>  7

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Ala Asp Ala Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Arg Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  8
<211>  611
<212>  PRT
<213>  marine polymerase modified by mutagenesis

<400>  8

Phe Asp Lys Ser Lys Tyr Glu Cys Val Gln Asp Val Glu Arg Leu Gln 
1               5                   10                  15      


His Trp Val Asp Arg Cys Thr Asp Val Gly Tyr Cys Ala Val Asp Leu 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Ser Ala Ala Ala Asn Leu Val Gly Val Cys 
        35                  40                  45              


Leu Ala Val Ala Asp Asn Glu Ala Cys Tyr Ile Pro Leu Gly His Thr 
    50                  55                  60                  


Gly Gly Gly Asp Leu Leu Gly Asp Gly Ala Pro Glu Gln Ile Pro Met 
65                  70                  75                  80  


Gln Thr Ala Leu Asp Val Leu Glu Pro Met Leu His Asn Ala Ala Val 
                85                  90                  95      


Leu Lys Ile Gly Gln Asn Phe Lys Tyr Asp Leu Gly Val Phe Gln Arg 
            100                 105                 110         


Tyr Gly Leu Gln Pro Ala Pro Tyr Asp Asp Thr Met Leu Ile Ser Tyr 
        115                 120                 125             


Ala Leu Ser Cys Gly Leu His Ser His Gly Met Asp Asn Leu Ser Glu 
    130                 135                 140                 


Met Tyr Phe Asp His Lys Pro Ile Pro Phe Lys Glu Leu Val Gly Ser 
145                 150                 155                 160 


Gly Lys Ser Gln Lys Thr Phe Asn Gln Leu Ser Leu Glu Glu Ser Thr 
                165                 170                 175     


Pro Tyr Ala Ala Glu Asp Ala Asp Val Thr Leu Arg Leu Trp Lys Leu 
            180                 185                 190         


Leu Lys Pro Arg Leu Ala Ser Glu Asn Val Ala Ser Val Tyr Glu Thr 
        195                 200                 205             


Leu Glu Arg Gly Met Pro Ser Val Leu Ala Met Met Glu Asn Asn Gly 
    210                 215                 220                 


Ile Lys Val Asp Lys Ala Val Leu Ala Arg Leu Ser Gly Asp Phe Glu 
225                 230                 235                 240 


Gln Lys Lys Ala Gly Leu Glu Ala Glu Ala His Glu Leu Ala Gly Arg 
                245                 250                 255     


Ser Phe Asn Leu Gly Ser Pro Lys Gln Leu Gly Glu Ile Leu Phe Asp 
            260                 265                 270         


Glu Leu Gly Leu Ser Gly Gly Lys Lys Thr Lys Thr Gly Ala Trp Gln 
        275                 280                 285             


Thr Gly Ala Gly Ile Leu Glu Ala Leu Glu His Val His Pro Leu Pro 
    290                 295                 300                 


Lys Ala Ile Leu Glu Trp Arg His Tyr Ala Lys Leu Lys Ser Thr Tyr 
305                 310                 315                 320 


Thr Asp Thr Leu Pro Gln Gln Ile Asn Ala Arg Thr Gly Arg Val His 
                325                 330                 335     


Thr Ser Tyr Ser Leu Ala Ser Thr Ser Thr Gly Arg Leu Ser Ser Ser 
            340                 345                 350         


Asp Pro Asn Leu Gln Asn Ile Pro Ile Arg Thr Glu Asp Gly Arg Lys 
        355                 360                 365             


Ile Arg Thr Ala Phe Ile Ala Glu Pro Gly Asn Ile Leu Val Ala Ala 
    370                 375                 380                 


Asp Tyr Ser Gln Val Glu Leu Arg Ile Leu Ala His Val Ala Asp Leu 
385                 390                 395                 400 


Thr Asn Met Lys Gln Ala Phe Ala Asp Gly Val Asp Ile His Ala Leu 
                405                 410                 415     


Thr Ala Ser Glu Met Phe Gly Val Pro Ile Asp Gly Met Asp Ser Ser 
            420                 425                 430         


Val Arg Arg Arg Ala Lys Ala Ile Asn Phe Gly Ile Ile Tyr Gly Ile 
        435                 440                 445             


Ser Ala Phe Gly Leu Ala Asn Asn Leu Gly Ile Ser Arg Thr Glu Ala 
    450                 455                 460                 


Lys Glu Tyr Ile Asp Ser Tyr Phe Glu Lys Phe Pro Gly Ile Lys Thr 
465                 470                 475                 480 


Tyr Met Glu Ser Ala Lys Asp Glu Ala Arg Glu Asn Gly Phe Val Lys 
                485                 490                 495     


Thr Ile Phe Gly Arg Lys Cys His Ile Lys Gly Ile Asn Asp Lys Asn 
            500                 505                 510         


Gly Ala Met Arg Gly Phe Gly Glu Ala Gln Ala Ile Asn Ala Pro Ile 
        515                 520                 525             


Gln Gly Ala Ala Ala Asp Ile Met Arg Arg Ala Met Ile Arg Met Pro 
    530                 535                 540                 


Asn Ala Ile Ser Asn Ile Glu Asn Ala Arg Met Leu Leu Gln Val His 
545                 550                 555                 560 


Asp Glu Leu Val Phe Glu Val Pro Glu Ala Ser Ala Glu Ala Leu Ile 
                565                 570                 575     


Lys Thr Val Lys Ser Val Met Gln Asn Ala Cys Ala Pro Ala Val His 
            580                 585                 590         


Leu Ser Val Pro Leu Val Val Asp Ala Lys Ala Ala His Asn Trp Asn 
        595                 600                 605             


Asp Ala His 
    610     


<210>  9
<211>  1836
<212>  DNA
<213>  marine polymerase with optimized codon

<400>  9
ttcgacaaaa gcaaatatga gtgcgttcag gatgttgaac gtctgcagca ttgggttgat       60

cgttgtaccg atgttggtta ttgtgcagtt gatctggaaa ccgatagcct ggatagcgca      120

gcagcaaatc tggttggtgt ttgtctggca gttgcagata atgaagcatg ttatattccg      180

ctgggtcata ccggtggtgg tgatctgctt ggtgatggtg caccggaaca aattccgatg      240

cagaccgcac tggatgttct ggaaccgatg ctgcataatg ccgcagttct gaaaattggc      300

cagaacttca aatatgatct gggtgtgttt cagcgttatg gtctgcagcc tgcaccgtat      360

gatgatacca tgctgattag ctatgcactg agctgtggtc tgcatagcca tggtatggat      420

aatctgagcg aaatgtattt cgaccataaa ccgattccgt tcaaagaact ggttggtagc      480

ggtaaaagcc agaaaacctt taatcagctg agcctggaag aaagcacccc gtatgcagcc      540

gaagatgcag atgttaccct gcgtctgtgg aaactgctga aaccgcgtct ggcaagcgaa      600

aatgttgcaa gcgtttatga aaccctggaa cgtggtatgc cgagcgttct ggcaatgatg      660

gaaaataatg gtatcaaagt ggataaagcg gttctggcac gtctgagcgg tgattttgaa      720

cagaaaaaag caggtctgga agccgaagca catgaactgg caggtcgttc atttaatctg      780

ggtagcccga aacagctggg tgaaattctg tttgatgaac tgggtctgag tggtggcaaa      840

aaaaccaaaa ccggtgcatg gcagaccggt gcaggtattc tggaagcact ggaacatgtg      900

catccgctgc cgaaagcaat tctggaatgg cgtcattatg caaaactgaa aagcacctat      960

accgatacac tgccgcagca gattaatgca cgtaccggtc gtgttcatac cagctatagc     1020

ctggcaagca ccagcaccgg tcgtctgagc agcagcgatc cgaatctgca gaatattccg     1080

attcgtaccg aagatggtcg taaaattcgc accgcattta ttgcagaacc gggtaatatt     1140

ctggttgcag ccgattatag ccaggttgaa ctgcgtattc tggcacatgt tgcagatctg     1200

accaatatga aacaggcatt tgcagatggt gttgatattc atgcactgac cgcaagtgaa     1260

atgtttggtg ttccgattga tggcatggat agcagcgttc gtcgtcgtgc aaaagcaatt     1320

aactttggta ttatctatgg catcagcgca tttggtctgg caaataatct gggcattagc     1380

cgcaccgaag caaaagaata tatcgatagc tacttcgaga agttccctgg cattaaaacc     1440

tatatggaaa gcgcaaaaga tgaagcccgt gaaaatggtt ttgtgaaaac gatttttggt     1500

cgcaagtgcc atattaaagg catcaacgat aaaaatggtg ccatgcgtgg ttttggtgaa     1560

cgtcaggcaa ttaacgcacc gattcagggt gcagcagcag atattatgcg tcgtgccatg     1620

attcgtatgc cgaatgccat tagcaatatt gaaaatgccc gtatgctgct tcaggttcat     1680

gatgagctgg tttttgaagt gccggaagca agcgcagaag cactgattaa aaccgttaaa     1740

agcgttatgc agaatgcatg tgcaccggca gttcatctga gcgttccgct ggttgttgat     1800

gcaaaagccg cacataattg gaatgatgcc cattaa                               1836


                         

<210>  10
<211>  810
<212>  PRT
<213>  Moritella viscosa


<220>
<221>  misc_feature
<222>  (442)..(442)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (568)..(568)
<223>  Xaa can be any naturally occurring amino acid

<400>  10

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Xaa Phe Lys Xaa Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Xaa Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  11
<211>  810
<212>  PRT
<213>  Moritella viscosa

<400>  11

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Asp Phe Lys Ser Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Asp Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  12
<211>  810
<212>  PRT
<213>  Moritella viscosa modified by mutagenesis

<400>  12

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Ala Phe Lys Ser Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Asp Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  13
<211>  810
<212>  PRT
<213>  Moritella viscosa modified by mutagenesis

<400>  13

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Asp Phe Lys Ser Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Ala Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  14
<211>  810
<212>  PRT
<213>  Moritella viscosa modified by mutagenesis

<400>  14

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Glu Phe Lys Ser Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Asp Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  15
<211>  810
<212>  PRT
<213>  Moritella viscosa modified by mutagenesis

<400>  15

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Asp Phe Lys Ser Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Glu Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  16
<211>  810
<212>  PRT
<213>  Moritella viscosa modified by mutagenesis

<400>  16

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Ala Phe Lys Ser Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Ala Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  17
<211>  810
<212>  PRT
<213>  Moritella viscosa

<400>  17

Met Ser Ala Thr Tyr Leu Gly Phe Leu Leu Ser Arg His Ser Arg Asp 
1               5                   10                  15      


Arg Asn Gly Asn Asn Glu Leu Ser Tyr Trp Leu Ala Ser Glu Met Gly 
            20                  25                  30          


Ala Val Lys Leu Ile Pro Ala Thr Gln Gln Leu Val Met Phe Ile Pro 
        35                  40                  45              


Gln Asp Gln Leu Thr Thr Ala Leu Ala Cys Leu Ser Glu Leu Asn Pro 
    50                  55                  60                  


Arg Phe Thr Phe Lys Asp Leu Lys Met Arg Ser Phe Asp Leu Glu Leu 
65                  70                  75                  80  


Met Ser Ala Phe Tyr Phe Arg Thr Ser His Asp Phe His Arg Ala Gln 
                85                  90                  95      


Glu Leu Leu Arg Arg Lys Leu Val Thr Val Leu Glu Ala Asp Ile Arg 
            100                 105                 110         


Pro Pro Glu Arg Tyr Leu Met Glu Arg Phe Ile Lys Gly Ala Val Glu 
        115                 120                 125             


Phe Thr Gly Thr Pro Val Gln Arg Lys Gly Tyr Val Glu Phe Gln Gln 
    130                 135                 140                 


Ala Gln Leu Lys Pro Ala Glu Leu Pro Asp Asn Leu Val Asp Lys Ile 
145                 150                 155                 160 


Lys Gln Ile Ser Leu Asp Ile Glu Cys Ser Glu His Gly Glu Leu Tyr 
                165                 170                 175     


Ser Ile Gly Leu Tyr Ser Leu Ser Pro Ala Tyr Cys Pro Asp Gly Gln 
            180                 185                 190         


Pro Phe Lys Arg Val Tyr Met Ile Gly Glu Gln Pro Glu Arg Asp Leu 
        195                 200                 205             


Ala Gln Thr Pro Glu Ser Glu Pro Leu Ile His Trp Val Ala Asp Glu 
    210                 215                 220                 


Lys Ser Leu Leu Leu Ala Leu Gln Ala Phe Ala Ile Ser Tyr Asp Pro 
225                 230                 235                 240 


Asp Ile Phe Ile Gly Trp Asn Val Ile Asn Phe Asp Phe Arg Leu Leu 
                245                 250                 255     


Ala Gln Arg Ala Thr Phe His Asn Leu Lys Leu Ala Leu Gly Arg Gly 
            260                 265                 270         


Gly Gln Asn Leu His Trp Arg Asp Gly Arg Thr Pro Gln Gln Gln Gly 
        275                 280                 285             


Phe Leu Thr Leu His Gly Arg Val Val Val Asp Gly Ile Asp Ser Leu 
    290                 295                 300                 


Lys Thr Ala Thr Tyr Ser Phe Pro Ser Phe Ser Leu Glu Asn Val Ala 
305                 310                 315                 320 


Gln Glu Ile Leu Gly Val Gly Lys Asp Thr Asp Asp Val Asp Asn Arg 
                325                 330                 335     


Met Glu Gln Ile Asn His Asp Phe His Phe Asn Lys Val Lys Leu Ala 
            340                 345                 350         


Lys Tyr Asn Leu Gln Asp Cys Val Leu Val Trp Asp Ile Phe Val Lys 
        355                 360                 365             


Thr Arg Leu Leu Asp Phe Leu Leu Leu Arg Ser Gln Leu Thr Gly Leu 
    370                 375                 380                 


Glu Leu Asp Arg Asn Gly Gly Ser Val Leu Ala Phe Thr Asn Val Tyr 
385                 390                 395                 400 


Leu Pro Lys Leu His Arg Ala Gly Tyr Ile Ala Pro Asn Leu Arg Glu 
                405                 410                 415     


Ser Gly Val Met Ala Ser Pro Gly Gly Tyr Val Met Asp Ser Phe Pro 
            420                 425                 430         


Gly Leu Tyr Asp His Val Ile Val Leu Asp Phe Lys Arg Leu Tyr Pro 
        435                 440                 445             


Ser Ile Ile Arg Thr Phe Lys Ile Asp Pro Val Gly Leu Leu Glu Gly 
    450                 455                 460                 


Val Gln Asn Pro Thr Glu Ala Ile Pro Gly Phe Arg Gly Gly Leu Phe 
465                 470                 475                 480 


Asp Arg Glu Lys His Tyr Leu Pro Asp Ile Ile Thr Glu Leu Trp Ser 
                485                 490                 495     


Gln Arg Asp Gln Ala Lys Leu Asp Lys Asp Ala Ala Arg Ser Gln Ala 
            500                 505                 510         


Ile Lys Ile Leu Met Asn Ser Phe Tyr Gly Val Leu Gly Ser Gly Gly 
        515                 520                 525             


Cys Arg Phe Tyr Asp Thr Arg Leu Ala Ser Ser Ile Thr Met Arg Gly 
    530                 535                 540                 


Gln Glu Ile Met Gln Thr Thr Ala Lys Trp Ile Glu Glu Gln Gly Tyr 
545                 550                 555                 560 


Lys Val Ile Tyr Gly Asp Thr Asp Ser Thr Phe Val Leu Leu Asp Ala 
                565                 570                 575     


Ala Lys Phe Thr Glu Gly Asp Arg Ser Glu Gln Ala Asp Arg Met Gly 
            580                 585                 590         


Lys Glu Leu Ser Glu Tyr Ile Asn Gln Gln Trp Gln Arg His Leu Arg 
        595                 600                 605             


Glu Asp Tyr Asp Ile Asp Cys Phe Leu Asp Ile Glu Tyr Glu Val His 
    610                 615                 620                 


Tyr His Lys Phe Leu Met Pro Thr Ile Arg Gly Leu Asp Lys Gly Ser 
625                 630                 635                 640 


Lys Lys Arg Tyr Ala Gly Leu Val Asn Thr Lys Asp Gly Glu Lys Leu 
                645                 650                 655     


Ile Phe Lys Gly Leu Glu Thr Val Arg Thr Asp Trp Thr Asp Leu Ala 
            660                 665                 670         


Lys Met Phe Gln Met Glu Leu Tyr His Arg Val Phe His Gly Leu Ala 
        675                 680                 685             


Val Glu Asp Tyr Val Leu Glu Ile Val Glu Arg Thr Leu Ala Gly Glu 
    690                 695                 700                 


Phe Asn Asp Lys Leu Val Tyr Arg Lys Arg Leu Arg Gln Glu Leu Ser 
705                 710                 715                 720 


Ala Tyr Val Lys Asn Val Pro Pro His Val Lys Ala Ala Arg Ala Ala 
                725                 730                 735     


Asp Glu Lys Asn Arg Gln Leu Gly Gln Pro Leu Arg Tyr Gln His Lys 
            740                 745                 750         


Ala Trp Ile Ser Tyr Val Leu Thr Leu Ser Gly Pro Glu Ala Val Glu 
        755                 760                 765             


His Gln His Ser Val Leu Asp Phe Glu His Tyr Ile Glu Lys Gln Ile 
    770                 775                 780                 


Lys Pro Ile Ala Asp Gly Ile Leu Pro Phe Ile Gly Leu Ser Phe Asp 
785                 790                 795                 800 


Leu Ile Thr Asp Gln Gln Met Gly Leu Phe 
                805                 810 


<210>  18
<211>  2433
<212>  DNA
<213>  Moritella viscosa

<400>  18
atgtctgcta catatctggg ttttttatta tcgcgccata gccgtgatcg taatggtaac       60

aatgaattaa gctactggtt agcatctgaa atgggtgcgg ttaaactgat cccggcaacc      120

cagcagctgg tgatgttcat tcctcaagat caacttacca ctgcattagc ttgtttaagt      180

gagttgaatc cgcgctttac ctttaaagac ttgaaaatgc gtagctttga tttagaactt      240

atgagcgcct tttatttccg tacttcccat gattttcacc gcgcacaaga gttattaaga      300

cgtaagctgg ttacggtatt agaagccgat attcgcccac ctgagcgtta cttaatggag      360

cgttttatca aaggcgcagt cgagttcact ggtacgcctg tacaacgtaa aggctatgtt      420

gaatttcaac aagcacagct taaaccagct gaactgccag ataacttagt cgataaaata      480

aaacagatct cactggatat cgaatgttca gaacacggag agctttactc tattggtttg      540

tattccctaa gccctgctta ttgcccagat ggtcagccct ttaaacgggt atacatgata      600

ggtgagcagc ctgagcgcga tttagcacaa actcctgaaa gcgagccgtt aatccattgg      660

gttgccgacg agaaaagttt attactggcg ctgcaagcat tcgcgatcag ttacgatccc      720

gacattttta ttggttggaa tgtaattaac tttgatttcc gtttattagc ccaacgtgcc      780

acattccata atcttaaatt agcattgggt cgaggtgggc aaaacctgca ttggcgagat      840

ggccgtaccc cacagcaaca aggctttctg actttacacg gtcgggtggt cgttgatggt      900

attgatagtt taaaaacggc aacttatagc ttcccaagtt ttagtttaga aaacgtggca      960

caagagattc tcggtgttgg taaagatact gatgatgtcg ataatcgcat ggaacagatt     1020

aaccacgact ttcattttaa caaggtaaaa ctggcgaaat acaacctgca agattgcgtc     1080

ttagtgtggg atatttttgt taaaacccgt ttgttagatt ttttattact acgctcgcag     1140

ttaaccggtt tagaactaga tcgtaacggt ggatcggtat tggcgtttac caatgtgtat     1200

cttcctaagt tacatcgcgc gggttatatc gcgccaaatc tgcgtgagag tggggtaatg     1260

gcaagtcctg gcggttatgt gatggattca tttcctggct tgtatgatca tgtcatcgta     1320

ctggatttta aaagtctgta tccgtcaatt attcgcacct ttaaaattga tccggtaggg     1380

ttgctagaag gggttcaaaa cccgactgag gcgattccgg gtttccgtgg tggtttgttt     1440

gatcgtgaaa agcattacct tcccgatatt attaccgaac tgtggtcgca gcgcgatcaa     1500

gccaaactcg ataaagatgc tgcccgttca caggccatta aaatcttaat gaattcgttt     1560

tatggtgtgt tagggtcggg cggttgtcgt ttttatgata ctcgtttagc ctcgtcgatc     1620

accatgcgtg ggcaagaaat catgcagact accgcaaaat ggatcgaaga gcagggatat     1680

aaggtgattt atggcgatac cgattccacc tttgtattac tcgatgccgc caagtttacc     1740

gagggtgatc gtagcgaaca agccgatcgc atgggcaaag agctgtcaga atatattaac     1800

cagcagtggc aacgacacct acgtgaagat tacgatatcg actgtttctt agatattgaa     1860

tacgaagtgc attatcacaa gtttttaatg ccgactatcc gtggcttgga taaaggcagt     1920

aaaaagcgct atgccggatt agtgaatacc aaagatggtg aaaaacttat ttttaaaggg     1980

ctggaaaccg tacgtaccga ttggactgac ttagccaaga tgttccaaat ggagttatac     2040

catcgggtat ttcatggctt agcagtcgaa gattatgtac tggaaattgt agaaaggacc     2100

ttagcgggtg agtttaacga taagttagtt tatcgtaagc gtttacgcca agaattgtct     2160

gcttatgtga agaacgtgcc gccacatgta aaagcggcgc gtgctgctga tgagaagaat     2220

cgccaattag ggcaaccgtt acgttatcag cataaagcgt ggatcagtta tgtgttaacc     2280

ctaagtggcc ctgaagctgt cgagcatcaa cattcggtac tcgattttga gcattacatt     2340

gaaaaacaaa ttaaacccat tgctgatggt atcttgcctt ttattggctt gagttttgat     2400

ttgataaccg atcaacaaat gggattattt taa                                  2433


<210>  19
<211>  25
<212>  DNA
<213>  primer

<400>  19
caccttgtct gctacatatc tgggt                                             25


<210>  20
<211>  32
<212>  DNA
<213>  primer

<400>  20
ttaaaataat cccatttgtt gatcggttat ca                                     32


<210>  21
<211>  82
<212>  DNA
<213>  molecular beacon template

<400>  21
ggcccgtagg aggaaaggac atcttctagc atacgggccg tcaagttcat ggccagtcaa       60

gtcgtcagaa atttcgcacc ac                                                82


<210>  22
<211>  19
<212>  DNA
<213>  primer

<400>  22
gtggtgcgaa atttctgac                                                    19


<210>  23
<211>  40
<212>  DNA
<213>  primer

<400>  23
tatccaccaa tactacccta cgatactttg tccactcaat                             40


<210>  24
<211>  40
<212>  DNA
<213>  primer

<400>  24
ataggtggtt atgatgggat gctatgaaac aggtgagtta                             40


<210>  25
<211>  39
<212>  DNA
<213>  primer

<400>  25
tatccaccaa tactaccctc gatactttgt ccactcaat                              39


<210>  26
<211>  40
<212>  DNA
<213>  primer

<400>  26
ataggtggtt atgatgggat gctatgaaac aggtgagtta                             40


