                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals S.A.
 
<120>  Immunogenic Compositions

<130>  VB66722 WO

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  264
<212>  PRT
<213>  Bordetella pertussis

<400>  1

Met Ser Gly Asn Ile His Pro Thr Ala Val Val Asp Pro Ala Ala Gln 
1               5                   10                  15      


Ile Asp Ser Ser Val Val Ile Gly Pro Tyr Ser Val Val Gly Pro Gly 
            20                  25                  30          


Val Ser Ile Ala Ala Gly Thr Glu Val Gly Ala His Cys Val Leu Asp 
        35                  40                  45              


Gly Val Thr Ser Ile Gly Arg Asp Asn Arg Phe Tyr Arg Phe Cys Ser 
    50                  55                  60                  


Ile Gly Gly Met Pro Gln Asp Lys Lys Tyr Ser Gly Glu Pro Thr Arg 
65                  70                  75                  80  


Leu Val Ile Gly Asp Arg Asn Thr Val Arg Glu Phe Thr Thr Phe Asn 
                85                  90                  95      


Thr Gly Thr Val Gln Asp Gly Gly Val Thr Ser Ile Gly Asp Asp Asn 
            100                 105                 110         


Trp Ile Met Ala Tyr Val His Ile Ala His Asp Cys His Ile Gly Asn 
        115                 120                 125             


Asn Thr Ile Leu Ala Asn Ser Val Gln Leu Gly Gly His Val Gln Val 
    130                 135                 140                 


Gly Asp Trp Ala Ile Val Gly Gly Leu Thr Gly Val His Gln Phe Ala 
145                 150                 155                 160 


Lys Ile Gly Ala His Ser Met Thr Gly Gly Asn Ser Ser Leu Met Gln 
                165                 170                 175     


Asp Ala Pro Pro Phe Val Leu Ala Ala Gly Asn Pro Cys Arg Pro Val 
            180                 185                 190         


Gly Val Asn Val Glu Gly Leu Lys Arg Arg Gly Phe Ser Ala Ala Ala 
        195                 200                 205             


Ile Ser Ala Leu Arg Asp Ala Tyr Lys Ser Ile Tyr Arg Arg Gly Leu 
    210                 215                 220                 


Ser Leu Asp Glu Gly Arg Ala Glu Leu Arg Ala Arg Gln Gln Ala Glu 
225                 230                 235                 240 


Pro Asp Val Ala Glu His Leu Gln Thr Met Leu Asp Phe Leu Asp Ala 
                245                 250                 255     


Ser Thr Arg Gly Ile Ile Arg Pro 
            260                 


<210>  2
<211>  264
<212>  PRT
<213>  Bordetella parapertussis

<400>  2

Met Ser Gly Asn Ile His Pro Thr Ala Val Val Asp Pro Ala Ala Gln 
1               5                   10                  15      


Ile Asp Ser Ser Val Val Ile Gly Pro Tyr Ser Val Val Gly Pro Gly 
            20                  25                  30          


Val Ser Ile Ala Ala Gly Thr Glu Val Gly Ala His Cys Val Leu Asp 
        35                  40                  45              


Gly Val Thr Ser Ile Gly Arg Asp Asn Arg Phe Tyr Arg Phe Cys Ser 
    50                  55                  60                  


Ile Gly Gly Met Pro Gln Asp Lys Lys Tyr Ser Gly Glu Pro Thr Arg 
65                  70                  75                  80  


Leu Val Ile Gly Asp Arg Asn Thr Val Arg Glu Phe Thr Thr Phe Asn 
                85                  90                  95      


Thr Gly Thr Val Gln Asp Gly Gly Val Thr Ser Ile Gly Asp Asp Asn 
            100                 105                 110         


Trp Ile Met Ala Tyr Val His Ile Ala His Asp Cys His Ile Gly Asn 
        115                 120                 125             


Asn Thr Ile Leu Ala Asn Ser Val Gln Leu Gly Gly His Val Gln Val 
    130                 135                 140                 


Gly Asp Trp Ala Ile Val Gly Gly Leu Thr Gly Val His Gln Phe Ala 
145                 150                 155                 160 


Lys Ile Gly Ala His Ser Met Thr Gly Ser Asn Ser Ser Leu Met Gln 
                165                 170                 175     


Asp Ala Pro Pro Phe Val Leu Ala Ala Gly Asn Pro Cys Arg Pro Val 
            180                 185                 190         


Gly Val Asn Val Glu Gly Leu Lys Arg Arg Gly Phe Ser Ala Ala Ala 
        195                 200                 205             


Ile Ser Ala Leu Arg Asp Ala Tyr Lys Ser Ile Tyr Arg Arg Gly Leu 
    210                 215                 220                 


Ser Leu Asp Glu Ala Arg Ala Glu Leu Arg Ala Arg Gln Gln Ala Glu 
225                 230                 235                 240 


Pro Asp Val Ala Glu His Leu Gln Thr Met Leu Asp Phe Leu Asp Ala 
                245                 250                 255     


Ser Thr Arg Gly Ile Ile Arg Pro 
            260                 


<210>  3
<211>  333
<212>  PRT
<213>  Comamonas testosteroni

<400>  3

Met Ser Val Leu Leu Gly Gln Ile Leu Asp Ala Leu Gly Gly Glu Leu 
1               5                   10                  15      


Val Gly Gly Glu Arg Glu Met Pro Ile Ser Arg Ile Ala Pro Leu Asp 
            20                  25                  30          


Ser Ala Gly His Gly Asp Leu Ser Phe Leu Ser Asn Pro Arg Tyr Arg 
        35                  40                  45              


Gln Gln Leu Ala Ala Ser Gln Ala Ala Cys Val Ile Val Ala Pro Thr 
    50                  55                  60                  


Met Arg Asp Glu Ala Ala Gln Arg Gly Ala Cys Ile Val Thr Ala Asp 
65                  70                  75                  80  


Pro Tyr Ala Tyr Phe Ala Arg Ala Thr Gln Trp Trp Lys Ala His Gln 
                85                  90                  95      


Gln Gly Gly Arg Pro Arg Gly Ile His Ala Ser Ala Val Val Asp Ala 
            100                 105                 110         


Thr Ala Gln Val His Glu Ser Ala Tyr Val Gly Pro Gln Cys Val Val 
        115                 120                 125             


Glu Ala Gly Ala Val Ile Gly Ala Asp Thr Val Leu Lys Ser Arg Val 
    130                 135                 140                 


Thr Ile Ser Gln Gly Cys Val Leu Gly Glu Arg Cys Ile Leu His Pro 
145                 150                 155                 160 


Gly Val Val Ile Gly Ala Asp Gly Phe Gly Phe Ala Pro Ser Ala Gly 
                165                 170                 175     


Gln Trp Glu Lys Ile Glu Gln Leu Gly Ala Val Arg Ile Gly Asn Asp 
            180                 185                 190         


Val Glu Ile Gly Ala Asn Thr Cys Val Asp Arg Gly Ala Leu Asp Asp 
        195                 200                 205             


Thr Val Ile Glu Asp Gly Val Lys Ile Asp Asn Leu Val Gln Ile Ala 
    210                 215                 220                 


His Asn Val His Ile Gly Ala His Thr Val Ile Ala Gly Asn Thr Gly 
225                 230                 235                 240 


Ile Ala Gly Ser Ala Arg Ile Gly Lys Arg Cys Gln Ile Gly Gly Ala 
                245                 250                 255     


Ala Asn Ile Leu Gly His Leu Thr Ile Ala Asp Gly Thr Val Ile Ser 
            260                 265                 270         


Pro Thr Ser Met Val Thr Arg Ser Leu Pro Lys Ala Gly Phe Tyr Thr 
        275                 280                 285             


Gly Ile Phe Pro Leu Gln Glu Asn Glu Gln Trp Glu Lys Asn Ala Ala 
    290                 295                 300                 


Thr Phe Arg Gln Leu Tyr Thr Leu Arg Glu Arg Val Lys Lys Leu Glu 
305                 310                 315                 320 


Gln Ala Leu Ala Glu Gly Arg Gln Ser Asn Asn Gly Asn 
                325                 330             


<210>  4
<211>  353
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  4

Met Met Ser Thr Leu Ser Tyr Thr Leu Gly Gln Leu Ala Ala His Val 
1               5                   10                  15      


Gly Ala Glu Val Arg Gly Asp Ala Asp Leu Pro Ile Gln Gly Leu Ala 
            20                  25                  30          


Thr Leu Gln Glu Ala Gly Pro Ala Gln Leu Ser Phe Leu Ala Asn Pro 
        35                  40                  45              


Gln Tyr Arg Lys Tyr Leu Pro Glu Ser Arg Ala Gly Ala Val Leu Leu 
    50                  55                  60                  


Thr Ala Ala Asp Ala Asp Gly Phe Ala Gly Thr Ala Leu Val Val Ala 
65                  70                  75                  80  


Asn Pro Tyr Leu Ala Tyr Ala Ser Leu Ser His Leu Phe Asp Arg Lys 
                85                  90                  95      


Pro Lys Ala Ala Ala Gly Ile His Pro Thr Ala Ile Val Ala Ala Asp 
            100                 105                 110         


Ala Glu Val Asp Pro Ser Ala Ser Val Gly Ala Tyr Ala Val Ile Glu 
        115                 120                 125             


Ser Gly Ala Arg Ile Gly Ala Gly Val Ser Ile Gly Ala His Cys Val 
    130                 135                 140                 


Ile Gly Ala Arg Ser Val Ile Gly Glu Gly Gly Trp Leu Ala Pro Arg 
145                 150                 155                 160 


Val Thr Leu Tyr His Asp Val Thr Ile Gly Ala Arg Val Ser Ile Gln 
                165                 170                 175     


Ser Gly Ala Val Ile Gly Gly Glu Gly Phe Gly Phe Ala Asn Glu Lys 
            180                 185                 190         


Gly Val Trp Gln Lys Ile Ala Gln Ile Gly Gly Val Thr Ile Gly Asp 
        195                 200                 205             


Asp Val Glu Ile Gly Ala Asn Thr Thr Ile Asp Arg Gly Ala Leu Ser 
    210                 215                 220                 


Asp Thr Leu Ile Gly Asn Gly Val Lys Leu Asp Asn Gln Ile Met Ile 
225                 230                 235                 240 


Ala His Asn Val Gln Ile Gly Asp His Thr Ala Met Ala Ala Cys Val 
                245                 250                 255     


Gly Ile Ser Gly Ser Ala Lys Ile Gly Arg His Cys Met Leu Ala Gly 
            260                 265                 270         


Gly Val Gly Leu Val Gly His Ile Glu Ile Cys Asp Asn Val Phe Val 
        275                 280                 285             


Thr Gly Met Thr Met Val Thr Arg Ser Ile Thr Glu Pro Gly Ser Tyr 
    290                 295                 300                 


Ser Ser Gly Thr Ala Met Gln Pro Ala Ala Glu Trp Lys Lys Ser Ala 
305                 310                 315                 320 


Ala Arg Ile Arg Gln Leu Asp Asp Met Ala Arg Arg Leu Gln Gln Leu 
                325                 330                 335     


Glu Lys Arg Leu Ala Ala Val Thr Ser Ser Gly Asp Ala Ser Ser Asp 
            340                 345                 350         


Ala 
    


<210>  5
<211>  178
<212>  PRT
<213>  Bordetella bronchiseptica

<400>  5

Met Gln Phe Leu Lys Lys Asn Lys Pro Leu Phe Gly Ile Val Thr Leu 
1               5                   10                  15      


Ala Leu Ala Cys Ala Thr Ala Gln Ala Gln Pro Thr Gln Gly Gly Val 
            20                  25                  30          


Ser Leu His Tyr Gly Ile Gly Asp His Tyr Gln Arg Val Thr Leu Asn 
        35                  40                  45              


Tyr Glu Thr Pro Thr Leu Trp Ser His Gln Phe Gly Gly Asn Trp Gly 
    50                  55                  60                  


Arg Leu Asp Leu Thr Pro Glu Leu Gly Ala Ser Tyr Trp Trp Ala Asp 
65                  70                  75                  80  


Gly Ser Arg Ser Pro Gly His Val Trp Gln Ala Ser Ala Ile Pro Met 
                85                  90                  95      


Phe Arg Trp Trp Thr Gly Glu Arg Phe Tyr Ile Glu Ala Gly Ile Gly 
            100                 105                 110         


Ala Thr Val Phe Ser Ser Thr Ser Phe Ala Asp Lys Arg Ile Gly Ser 
        115                 120                 125             


Ala Phe Gln Phe Gly Asp His Ile Gly Leu Gly Phe Leu Leu Thr Pro 
    130                 135                 140                 


Ser Asn Arg Ile Gly Leu Arg Tyr Ser His Phe Ser Asn Ala Gly Ile 
145                 150                 155                 160 


Lys Glu Pro Asn Pro Gly Leu Asp Ile Val Gln Leu Thr Tyr Thr Tyr 
                165                 170                 175     


Gln Phe 
        


<210>  6
<211>  239
<212>  PRT
<213>  Francisella novicida

<400>  6

Met Leu Lys Gln Thr Leu Gln Thr Asn Phe Gln Gly Phe Lys Asp Ile 
1               5                   10                  15      


Phe Lys Lys Pro Lys Leu His Asn His Lys Leu Pro Arg Tyr Leu Gln 
            20                  25                  30          


Leu Lys Tyr Thr Phe Ile Pro Leu Leu Ile Leu Val Ile Phe Ala Tyr 
        35                  40                  45              


Tyr Asn Leu Asp Thr Pro Val Glu Asn Tyr Ile Lys His Ser Met Pro 
    50                  55                  60                  


Asn Ile Val Gly Val Ile Phe Gly Lys Ile Thr Asp Val Gly Lys Ala 
65                  70                  75                  80  


Glu Tyr Ile Leu Ile Ile Cys Gly Val Ile Val Leu Ala Arg Leu Phe 
                85                  90                  95      


Thr Asp Ser Gln Lys Leu Ser Ala Asn Thr Arg Ala Met Phe Asp Lys 
            100                 105                 110         


Val Ser Ala Tyr Ala Gly Phe Ile Leu Ala Thr Val Ala Ile Ser Gly 
        115                 120                 125             


Ile Leu Gly Gln Ile Leu Lys Met Ile Ile Gly Arg Ala Arg Pro Lys 
    130                 135                 140                 


Phe Phe Leu Glu Tyr Gly Ser His Tyr Phe Gln His Phe His Ala Pro 
145                 150                 155                 160 


Gly Tyr Asp Phe Ala Ser Met Pro Ser Gly His Ser Ile Thr Val Gly 
                165                 170                 175     


Ala Met Phe Ile Ala Phe Phe Tyr Ile Phe Pro Lys Leu Arg Tyr Phe 
            180                 185                 190         


Trp Tyr Leu Leu Ile Val Val Phe Ala Gly Ser Arg Ile Met Val Gly 
        195                 200                 205             


Ser His Tyr Pro Ser Asp Val Ile Phe Gly Val Ala Phe Gly Cys Tyr 
    210                 215                 220                 


Cys Thr Ala Tyr Ile Tyr Tyr Trp Met Arg Asn Arg Glu Ile Ile 
225                 230                 235                 


<210>  7
<211>  226
<212>  PRT
<213>  Rhizobium leguminosarum

<400>  7

Met Pro Pro Leu Arg Trp Gln Ala Cys Leu Leu Ile Thr Leu Asn Ala 
1               5                   10                  15      


Val Ile Leu Ser Met Leu Leu Phe Asp Ala Pro Ile Gly Ala Ser Glu 
            20                  25                  30          


Ala Pro Ala Pro Val Lys His Leu Gly Glu Ile Leu Thr Gly Phe Gly 
        35                  40                  45              


Asp Ser Ala Trp Leu Ile Tyr Thr Ser Ile Leu Leu Phe Phe Gln Gly 
    50                  55                  60                  


Arg Ala Gly Tyr Lys Leu Val Lys Thr Ala Arg Ser Lys Ala Gln Ala 
65                  70                  75                  80  


Leu Tyr Val Ser Trp Ile Gly Ala Tyr Leu Phe Ile Thr Val Val Phe 
                85                  90                  95      


Ser Gly Leu Leu Ala Asn Leu Leu Lys Arg Ala Ile Gly Arg Ala Arg 
            100                 105                 110         


Pro Asp His Phe His Asp Tyr Gly Met Phe Ser Phe Thr Pro Phe Ser 
        115                 120                 125             


Gly His Ala Ala Phe Glu Ser Phe Pro Ser Gly His Ser Thr Thr Val 
    130                 135                 140                 


Gly Ala Phe Phe Ala Ala Phe Ala Leu Leu Phe Pro Arg Tyr Arg Ile 
145                 150                 155                 160 


Ala Phe Ile Ala Cys Ala Ile Trp Leu Gly Met Thr Arg Val Met Val 
                165                 170                 175     


Gly Ala His Tyr Pro Ser Asp Val Ile Ala Gly Leu Ala Phe Gly Ala 
            180                 185                 190         


Trp Phe Ser Leu Leu Thr Ala Ile Val Phe Ala Arg Cys Gly Leu Leu 
        195                 200                 205             


Phe Lys Leu Ala Pro Asp Gly Trp Pro Leu Ser Lys Arg Leu Phe Arg 
    210                 215                 220                 


Thr Ala 
225     


<210>  8
<211>  222
<212>  PRT
<213>  Francisella novicida

<400>  8

Met Ala Arg Phe His Ile Ile Leu Gly Leu Val Val Cys Phe Phe Ala 
1               5                   10                  15      


Trp Ile Phe Phe Leu Ile Phe Pro Asn Leu Asp Ile Gln Phe Ala Gly 
            20                  25                  30          


His Phe Tyr Asn Ser Ser Ala His Gln Phe Ile Gly Gly Tyr Asp Gly 
        35                  40                  45              


Phe Leu Gly Phe Leu His Trp Phe Ala Arg Phe Phe Pro Ile Phe Phe 
    50                  55                  60                  


Ser Ile Ile Val Ile Leu Phe Leu Leu Gly Ser Leu Phe Ile Asp Lys 
65                  70                  75                  80  


Phe Lys Ile Lys Tyr Arg Lys Ala Ile Phe Phe Ile Ala Val Cys Leu 
                85                  90                  95      


Trp Ile Gly Pro Gly Leu Val Val Asn Tyr Val Phe Lys Asp His Trp 
            100                 105                 110         


Gly Arg Pro Arg Pro Val Met Val Glu Gln Phe Asn Gly Asp Lys Ile 
        115                 120                 125             


Phe Gln Pro Pro Phe Val Ile Ser Ser Gln Cys Asp Lys Asn Cys Ser 
    130                 135                 140                 


Phe Val Cys Gly Asp Ala Ser Met Gly Phe Trp Leu Phe Ala Phe Met 
145                 150                 155                 160 


Pro Leu Leu Ala Thr Arg Lys Lys Lys Leu Val Ala Phe Ile Ala Ala 
                165                 170                 175     


Val Val Ala Gly Gly Gly Leu Gly Leu Met Arg Met Ser Gln Gly Gly 
            180                 185                 190         


His Phe Phe Ser Asp Val Val Phe Cys Gly Ile Phe Val Tyr Ile Ser 
        195                 200                 205             


Thr Trp Val Val Tyr Ala Leu Met Tyr Arg Lys Lys Glu Tyr 
    210                 215                 220         


<210>  9
<211>  795
<212>  DNA
<213>  Bordetella parapertussis

<400>  9
atgtcaggaa acatccatcc caccgccgtg gtcgatccgg cggcacagat cgacagttcg       60

gtcgtgatcg gcccatacag cgtggtgggg ccgggagtca gcattgccgc gggcaccgag      120

gtcggcgcgc actgcgtgct cgacggcgtg accagcatcg ggcgcgacaa ccgcttctac      180

cgcttttgct ccatcggcgg catgccgcag gacaagaagt acagcggcga accgacgcga      240

ctggtcatcg gcgaccgcaa cacggtgcgc gagttcacca cgttcaacac cggcacggtg      300

caggacggcg gcgtgaccag catcggcgac gacaactgga tcatggccta cgtgcacatc      360

gcgcacgact gccatatcgg caacaacacc atcctggcca actcggtgca gctgggcggc      420

cacgtgcagg tgggcgactg ggccatcgtg ggcggcctga ccggcgtgca ccagttcgcc      480

aagatcggcg cgcacagcat gaccggcagc aacagctcgc tgatgcagga tgcgcccccg      540

ttcgtgctgg cggcgggcaa tccgtgccgg cccgtgggcg tcaatgtcga aggcttgaag      600

cggcgcggtt tctcggcggc ggccatctcg gcgctgcgcg atgcgtacaa gtcgatctac      660

cggcgcggac tgtcgctgga cgaggcgcgc gccgagctgc gcgcgcgcca gcaggccgag      720

ccggatgtcg ccgagcatct gcagaccatg ctggacttcc tggacgcttc gacgcgcggc      780

atcatccggc catga                                                       795


<210>  10
<211>  1002
<212>  DNA
<213>  Comamonas testosteroni

<400>  10
gtgagcgttc tattgggaca gattctcgat gcgctcggtg gagaactggt aggtggcgag       60

cgcgagatgc ctatctcgcg catcgctcca ctggactctg cgggccacgg tgatctgagc      120

tttttaagca atccccgcta tcgccagcaa ctggccgcct cacaggcggc ctgtgtcatt      180

gtggcgccga cgatgcgtga tgaagcagcg cagcgtggcg cctgcattgt cacggctgat      240

ccttatgcct atttcgcgcg agccacccag tggtggaagg cgcatcagca gggcggcagg      300

ccgcgcggca tccatgcgag tgcggtggtg gatgccacgg cccaggtgca tgaaagtgcc      360

tatgtggggc cgcaatgcgt ggtggaggca ggcgcggtca tcggtgccga tacggtgctg      420

aaatcgcgcg tcaccatcag ccagggctgt gtgctgggcg agcgctgcat tctgcatccc      480

ggtgtggtca tcggtgccga cggcttcggc ttcgcgccct cggcgggcca atgggaaaag      540

atagagcagt tgggggccgt gcgcatcggc aacgatgtgg aaatcggtgc caacacctgc      600

gtggatcgtg gggcacttga tgacaccgtc attgaagatg gcgtcaagat cgataatctg      660

gtgcagattg cccacaatgt gcacattggt gcgcatacgg tgattgccgg caataccggt      720

attgccggca gtgcgcgcat cggcaagcgc tgccagattg gtggagccgc caatattctg      780

gggcatttga cgattgcgga tggcacggtg atttcgccga cctccatggt cactcgctcc      840

ttgcccaagg caggttttta tacaggcatc tttccgttgc aagagaacga gcaatgggaa      900

aagaacgctg caaccttcag gcagttgtac acgctccggg agcgggtcaa gaagcttgaa      960

caagcgctgg ctgagggccg gcaaagcaac aacggaaatt ga                        1002


<210>  11
<211>  1062
<212>  DNA
<213>  Pseudomonas aeruginosa

<400>  11
atgatgagta ccttgtccta caccctgggc cagttggccg cgcatgtcgg cgcggaagtc       60

cgtggcgacg cggacctgcc gatccagggc ctggcgacct tgcaggaggc cggaccggct      120

cagctgagct tcctggccaa cccgcagtat cgcaagtacc tgcccgaaag ccgtgccggc      180

gccgtcctgc tgacggctgc cgacgccgac gggttcgccg gtaccgcgct ggtggtggcc      240

aacccctacc tggcctatgc cagcctgtcg cacctgttcg atcgcaaacc gaaggcggcc      300

gccggtatcc atcctaccgc catcgtcgcg gcggacgccg aggtcgatcc gagcgccagc      360

gtcggcgcct atgcggtgat cgagagtggc gcccgcatcg gcgctggcgt cagcatcggc      420

gcgcactgcg tgatcggagc ccgtagcgtg atcggcgaag gcggctggct ggcgccgcga      480

gtcacgctct accacgacgt caccatcggc gcgcgggtga gtatccagtc gggcgcggtg      540

atcggcggcg aaggtttcgg tttcgccaac gagaagggcg tctggcagaa gatcgcccag      600

atcggcgggg tgaccatcgg tgacgacgtc gagatcggcg cgaacaccac catcgaccgt      660

ggagcactgt ccgatacgct gatcggcaac ggtgtgaagc tggacaacca gatcatgatc      720

gcgcacaacg tgcagatcgg cgatcatacc gccatggccg cttgcgtcgg catttccggc      780

agcgccaaga tcggcaggca ttgcatgctc gccggcggcg ttggcctggt cggccatatc      840

gagatctgcg acaacgtttt cgtgaccggc atgaccatgg tgacccgctc gatcaccgag      900

ccgggatcgt attcctccgg taccgccatg caaccggcgg ccgaatggaa aaaaagcgcc      960

gcgcgcattc gtcaactgga cgacatggcg cgtcgacttc agcagctgga aaaacgcctg     1020

gctgccgtga cctcaagcgg tgacgcttca tctgatgcgt aa                        1062


<210>  12
<211>  1002
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LpxD Comamonas testosterone (codon optimised)

<400>  12
atgagcgtgc tgctgggcca gatcctcgat gcgctgggcg gcgaactggt gggcggcgag       60

cgcgaaatgc ccatcagccg catcgcgccg ctggatagcg cgggccatgg cgatctgtcg      120

tttttaagca acccccgcta ccgccaacaa ctggccgcca gccaagccgc ttgtgtgatt      180

gtcgccccga ccatgcgcga cgaagccgcc cagcgcggcg cttgtatcgt gaccgccgat      240

ccgtacgcct atttcgcccg cgccacccag tggtggaaag cccaccagca aggcggccgc      300

ccgcgcggca tccacgccag cgccgtggtc gatgccaccg cccaagttca cgagagcgcg      360

tatgtcggcc cccagtgcgt cgtcgaggcc ggcgccgtga tcggcgcgga caccgtgctg      420

aagagccgcg tcaccatcag ccaaggttgc gttttaggcg agcggtgtat tttacatccg      480

ggcgtggtga tcggggccga tggcttcggc ttcgccccca gcgctggcca atgggaaaag      540

atcgagcagc tgggcgccgt gcgcatcggg aatgacgtgg agatcggcgc caacacgtgc      600

gtggaccgcg gcgcgctgga cgacacggtc atcgaggacg gcgtgaagat cgacaattta      660

gtgcagatcg cccacaacgt ccacatcggc gcgcacaccg tcatcgccgg caacaccggc      720

attgccggct cggcccgcat tggcaagcgc tgccagattg gcggggccgc caacatttta      780

ggccatttaa ccatcgccga tgggaccgtc atcagcccga cctcgatggt cacccgctcg      840

ctgcccaagg cgggcttcta caccggcatc ttcccgctcc aagaaaacga gcagtgggag      900

aagaatgcgg ccaccttccg ccagctgtac actttacgcg agcgcgtgaa gaaactggag      960

caagctctgg ccgaggggcg ccagagcaac aacggcaact ga                        1002


<210>  13
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LpxD Pseudomonas aeruginosa (codon optimised)

<400>  13
atgatgagca ctttaagcta cactttaggc cagctggccg cccatgtggg cgccgaggtc       60

cgcggcgacg ccgatctgcc cattcaaggt ttagccacgc tgcaagaagc cggcccggcg      120

cagctgagct ttttagcgaa cccccagtac cgcaagtacc tcccggagtc gcgcgccggc      180

gccgtgctgc tgaccgccgc ggacgccgac ggcttcgccg gcaccgcttt agtcgtggcc      240

aatccctatt tagcgtacgc ctctttatcg cacctcttcg atcgcaaacc gaaggccgcc      300

gcgggcatcc acccgaccgc gatcgtggcc gccgatgccg aagtggaccc gagcgcgagc      360

gtgggggcct atgccgtcat cgagagcggc gcccgcatcg gcgccggcgt gagcatcggc      420

gcgcattgcg tgattggcgc gcgcagcgtc attggcgaag gcggctggct ggcgccgcgc      480

gtgactttat accatgacgt gaccatcggc gctcgcgtca gcattcagag cggcgccgtc      540

atcgggggcg agggctttgg cttcgccaac gagaagggcg tctggcagaa aatcgcccaa      600

atcggcggcg tcaccatcgg cgacgacgtg gagattggcg ccaacaccac catcgatcgc      660

ggcgcgctgt cggacaccct catcggcaat ggcgtcaagc tggacaacca gattatgatc      720

gcgcacaacg tgcagatcgg cgaccatacc gccatggccg cttgtgtggg cattagcggc      780

agcgccaaga tcggccgcca ctgcatgctg gccggcggcg tgggtttagt gggccatatc      840

gagatttgcg acaacgtgtt cgtcaccggc atgaccatgg tgacccggag catcacggag      900

ccgggctcgt acagcagcgg caccgcgatg caaccggccg ccgagtggaa gaagtcggcc      960

gcccgcatcc gccaactgga tgacatggcg cgccgtttac aacagctgga gaagcgcctc     1020

gccgccgtga ccagcagcgg cgatgccagc agcgacgcct ga                        1062


<210>  14
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-term insert for genomic integration into B. pertussis

<400>  14
accatctgca agtaccaggg ccgggccctg gtcgacggcc aactggtcgc cgaagccaag       60

ctgatgtgcg cgatccgtag cctggaagag taa                                    93


<210>  15
<211>  491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-term insert for genomic integration into B. pertussis

<400>  15
gcctgcgcat aggcatggtc gccggcgagc cttcgggcga cctgctggcc gggcgcatca       60

tcgccggcct gcaggcccgg gcacccggcg tgcactgcgc cggcataggc gggccccaga      120

tggcggcgcg cggcttcgag gcctggcacc ccatgcatgc gctcacggtg ttcggctaca      180

tcgatgcgtt caagcggatt cccagcctgc tgtcgaccta cggcgacgtc aagcggcgcc      240

tgctggccga gccgccgtcg gtgttcgtgg gcatcgacgc gccggatttc aacctgcgcc      300

tggagcacca gttgcgccag gccggtaccc ccaccgtgca tttcgtcggt ccctcgatct      360

gggcctggcg ctacgaacgc atcaacaaga tccgcgccgc cgtctcccac atgctggtgc      420

tgtttccgtt cgaggaagcg ctctatcgca aggaaggaat cccggtcacc tatgtcggcc      480

atccgctggc c                                                           491


<210>  16
<211>  537
<212>  DNA
<213>  Bordetella bronchiseptica

<400>  16
atgcaatttc tcaagaaaaa caagcccctg ttcggcatcg ttacactggc tctggcatgt       60

gccaccgccc aggcgcagcc cactcagggc ggggtcagcc tgcattacgg tattggcgac      120

cactatcagc gcgtcacgct gaactacgaa acccccacgc tctggagcca ccagttcggc      180

ggcaattggg gccgcctgga cctgaccccc gaactgggcg cgtcatactg gtgggccgac      240

ggctcgcgct cgcccggcca cgtgtggcag gccagcgcca ttccgatgtt ccgctggtgg      300

accggcgagc gcttttacat cgaggccggc atcggcgcca cggttttcag cagcaccagc      360

ttcgccgaca agcgcatcgg ttcggccttc cagtttggcg accatatcgg gctgggcttc      420

ctgctgacgc ccagcaaccg catcggcctg cgctattcgc acttctccaa cgccggcatc      480

aaggaaccga accccggcct cgatatcgtg cagctgacct atacgtacca gttctga         537


<210>  17
<211>  296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Porin promoter (BP0840 promoter; BX640413.1:174494-174789)

<400>  17
tccttgagtg aactgggggg tcggcgattt ccagtttctc aaatccggtt cggatgaacc       60

atgcatacaa cctattgaat cttcacagtt agcccgcgcg cgattccgga ttaaggggac      120

aggatgttgc aacttaccaa caatggggcg ggaaaccggc ttttttctga gcctgaccat      180

agccagtcct gccgattttt gatgcaatag cgtcaacttc ccttcgcgga gttagggggg      240

gcggcaggcc ggtatgtgct gcattgctgc tcttgtcact caaatcaacg gagatt          296


<210>  18
<211>  1620
<212>  DNA
<213>  Bordetella pertussis

<400>  18
atgactctcg ctacccgatc catgcaaccc catgcggttt cccctggcca ggccaggtcc       60

tggcccctgc ccgcggcggg ctggctgctt ctcgcggtcg gcgtctggct ggccttcctg      120

tcctggatgc ggcccctggc gctgcccgac gaagggcgct acgccggcgt ggcctgggac      180

atgctgcgca acggctcgtt cgcggtgccg ctcatcgacg gcatgccgta tttccacaag      240

ccgccgctgt actactggct ggccgagctg tcgttccgcc tgttcggcgt caacgaatgg      300

gcggcgcgcc tgccctcggc gctggccgcc tgggccagcg ccgtcgcgct gtacctgttc      360

gtgcgccgcc atcgcgacgc cgcgtccgcg acgctctgcg tgctggtgct ggccacgctg      420

ccgctgtttt tcggcggcgc ccagtacgcc aacatggata tgctggtggc cggcatgatc      480

acgctgtgcg tgctggccgg cgccgacacg gcgctgcgtg tccgcggcgg gcaggcctgg      540

cgcgccatgg cgctggccac cggcgtgtgc gccgcgctgg ccatgctggc caaggggctg      600

atcgggctgg tgctgccggg cgcgatcctg ctggcctggc tggcgtggcg gcgcgactgg      660

cgcggcctgc gggccttgct gtggccgccc gccatcctgg cgttcgcggt ggtggccgtg      720

ccctggttct ggctgatgca ggtacgctac ccgggctttt tccagtactt cttcgtccac      780

cagcatttcg agcgtttcgc gcagaccggc ttcaacaatg tgcagccgtt ctggttctac      840

ctgcccgtca tcgcgggcct ggcattgccg tggtcgttgt gggccggcgg cttgctgcgc      900

aagcaattct gggccgccga cgccgacccg gacggcttgc gccgcctggc gctggtctgg      960

ctggcggtca tcgtggcgtt tttctccatg ccgcagtcca agctggtcgg ctacatcatg     1020

ccggtgctgc cgccgctggc cttcctgctg gccgaagtgg tgatgggcgc cttgcgcgac     1080

ccggcggtgg cgcgggccac gcgccgcatg gcgcgcgtca gcgccctggt ggcggtggcc     1140

atctgcgtga ccgcggtgtt cgtggcgtcg ttcaacgccc gcggcagttc gcgcgagctg     1200

gccctgtcgc tgcgcggcga gctgcggccg gacgacacgc tggtggccct gcatacctat     1260

ccgttcgacc tgcagctgta tgcccatgcg gcccggccga tgtgggtggt cgacgactgg     1320

agcaaccccg aaattcccaa gcgcgacaat tggcgcaggg agctctacga cgcggtccag     1380

ttcgagccgg ccctgggcga gcggctgctg gtctcggcag atactttcca gcagcgcctg     1440

tgccaggcgc cggagggcag ccgttactgg gtctggggca cggcggccga cgaagaggcg     1500

tacgcgccgc tgcgcggcca ggcggcgcgc tttgccgacg cgcggcgcag cctctggctg     1560

gtgctggtgg acgaagcctt caaggggcgt gtctgtgacg gaacgcccac aggcggctag     1620


<210>  19
<211>  4395
<212>  DNA
<213>  Bordetella pertussis

<400>  19
gtggataaag atgaatcggc attgcggcaa cttgtcgaca tggcgcttgt aggctacgac       60

ggcgtggtgg aagagttgct ggcgctgccc tcggaagagt ccggggatct tgcgggtgga      120

cgggccaaac gagagaaggc cgaattcgcg ctgtttagcg aagcgcccaa cggggatgaa      180

cccatcggcc aggatgctcg tacatggttc tattttccca agtaccgccc ggtggcggtt      240

tccaatttga agaaaatgca ggtggcgatt cgtgctcgtc tcgagcctga gtcattgatc      300

ctgcagtggt tgatcgcact cgatgtctat ctcggcgtgt tgatcgccgc cttgtcccgc      360

actgtgatca gcgacctggt attcgagtat gtcaaggcgc gctacgagat ctactaccta      420

ctgaatcgcg tgccgcatcc gctggcgacg gcgtacctca agcgccgccg ccagcgtccg      480

gtggatcgtt cgggacgtct gggctcggtg ttcgaacacc cgctatggtt cgcctacgac      540

gaattggccg gcaccgtcga tctggatgcc gacatctacg agcaggcgct cgccgaaagc      600

atcgaacgcc gcatggacgg agagccggac gacggcagtc tggataccgc cgaacacgac      660

gtatggcgtt tgtgccgcga cggcatcaat cggggcgagc aggcgatttt ccaggcatcc      720

ggcccatatg gcgtggtcgc ggatgcaggg tatatgcgca ccgttgccga tctggcgtat      780

gccgatgcat tggcggattg cctgcatgcc caactgcgca tccgggcgca aggctcggtc      840

gatagcccgg gagacgagat gccacgcaaa cttgatgcgt gggaaatcgc caagtttcat      900

ctggccgcga cccagcaggc gcgggttgat ctgctcgagg cggcgttcgc gctcgactac      960

gccgccttgc gcgatgtgcg cgtctacggc gattaccgca atgcgctggc tcttcggttc     1020

atcaagcgcg aggccttgcg gttgctgggg gcgcggcgcg gcaacgcctc tacgatgccg     1080

gcggttgcgg ctggcgagta cgacgaaatc gtggccagtg gagcagccaa tgacgcggct     1140

tatgtatcca tggccgccgc gttgatcgcg ggcgtgctct gcgatctcga gagcgcgcag     1200

cgcacgttgc ccgtcgtatt ggccaggttt cggccccttg gcgtgcttgc gcgattcaga     1260

aggctggagc aggaaaccgc gggcatgctg cttggcgacc aggagccgga gcctcggggc     1320

ttcatcagtt ttaccgattt tcgcgatagc gacgcgttcg ccagctacgc ggagtatgcg     1380

gcccagttca acgactatat cgatcaatac agcatactcg aggcgcagcg gctggcgcgg     1440

attctggccc tgggctcgcg gatgacggtc gatcaatggt gccttcccct gcagaaagta     1500

cggcactaca aggtgctgac atcgcagcca gggctgatcg cgcgtggaat cgaaaatcac     1560

aacaggggca ttgaatattg cctggggcgg ccgccgctga ccgatctgcc gggtcttttc     1620

accatgttcc agctccatga ttccagctgg ctgttggtat cgaacatcaa cggtgagctt     1680

tggtctgatg tccttgcgaa cgctgaggtg atgcagaatc ctacgctggc cgcccttgcc     1740

gagccgcaag gccgctttcg aaccggccgt cgaacgggcg ggtggtttct cggcggcccc     1800

gcgactgaag ggccaagtct gcgcgacaac tacctcctca aattgaggca gagcaatccg     1860

gggctggatg tcaagaaatg ctggtatttc gggtatcggc aagagtacag gctgcccgca     1920

ggcgcgctgg gtgtgccgct attcgctgtt tccgttgccc tgaggcacag tctggacgac     1980

ctcgcggcgc acgcgaagtc cgctttgtac aaacccagcg aatggcagaa gttcgccttc     2040

tggatcgtgc cgttctaccg ggaaatattc ttttcgaccc aggatcgttc ctatcgggtc     2100

gatgtgggga gcatcgtctt tgattcgatt tccttgcttg cctcggtgtt cagcatagga     2160

gggaagttgg gcagtttcac ccgtacccag tatggcaacc tgcgcaattt cgtggtgcgg     2220

caacgcatcg cggggttgag cgggcagcgc ttgtggcgtt cggtgctcaa ggagcttccg     2280

gcattgatcg gagccagcgg gttgcgcctg tcgcgttcgc tgctcgtcga tctgtatgag     2340

atcttcgagc ccgtgcctat ccgtcggctt gtcgcgggat tcgtgagcgc cactacggtc     2400

ggcgggcgta accaggcctt cctgcggcag gcattctccg ctgccagttc ctcggccggg     2460

cgcacggggg gccaattggc aagcgaatgg cggatggccg gcgtggacgc caccggtttg     2520

gtcgagtcca cgtcgggcgg caggtttgag ggcatctaca cgcgcggctt gggaccgttg     2580

agcgagtgca ccgagcactt catcgtcgaa tccgggaatg cctacagagt catctgggat     2640

gcatacacgc atggttggcg cgtggtcaat gggcgtttgc cgccgaggct gacctatacc     2700

gttccggttc ggctgaacgg gcaaggccac tgggagacgc atctggatgt ccctggccgg     2760

ggtggggcgc cggaaatctt cggacgcatc cgcacgcgta atctggtcgc ccttgccgcc     2820

gagcaggccg cgccgatgcg ccgcctgctc aaccaggcga ggcgtgtggc gctcaggcat     2880

atcgatacct gcaggagcag gcttgcgttg ccgcgcgctg aatccgatat ggacgcggcg     2940

atccggattt tcttcggaga gccggacgcc ggccttcgcc agcgcatcgg gcgacgcctg     3000

caggaggtca gggcctatat cggcgatctg agtccggtca atgacgtgct gtaccgggcg     3060

ggatatgacc tcgacgatgt cgcaacgctg tttaacgcag tggaccggaa cacgtcgctg     3120

ggcaggcagg ctcggatgga gttgtatctg gatgccattg tcgatttgca tgccaggctc     3180

ggctatgaaa atgcgcgttt tgtcgacctg atggcgttcc acctgctcag cctgggccat     3240

gccgcgacgg ccagtgaggt cgtggaggcc gtttcgcccc ggctgctggg caatgtgttc     3300

gatatctcga acgtcgccca gctcgaacgc ggtatcggga atcccgccag caccggtctt     3360

ttcgtcatgc tgggcgccta ttcggaatcg tcgcccgcga tattccagtc gttcgtgaac     3420

gatatatttc ccgcatggcg acaggcttcc ggcggggggc cgctggtatg gaacttcggt     3480

cctgccgcca tcagcccgac gcgcctggat tacgcaaata ccgatatcgg attgctcaac     3540

catggggata tttccccgct gcgcgccagg ccgccattgg gcggcaggcg cgacatcgat     3600

cttcctccgg ggctggatat ttcgttcgtg cgttacgacc gcccggtgcg catgtccgcg     3660

ccgcgtgcgc tcgacgccag cgtcttcagg ccggtcgacg ggcctgtcca tggctatatt     3720

caatcgtgga cgggggctga gatcgaatat gcgtacggcg cgcccgcagc ggcacgcgag     3780

gtcatgctga ccgacaatgt gcggatcatc agcatcgaga acggcgatga aggggcgatc     3840

ggggtgcgcg tgaggctcga cactgttccc gtcgcgacgc cgctcatcct gactggcggc     3900

tccttgagcg ggtgcacgac gatggttggg gtcaaggagg gctacctggc cttctaccac     3960

actggcaagt cgaccgaact cggggattgg gcgactgcgc gcgaaggcgt acaggcgctt     4020

taccaggccc atttggcgat ggggtacgcg ccgatctcaa tcccggcgcc gatgcgcaat     4080

gacgacttgg tcagcatcgc cgcgacctac gaccgggcgg tcattgccta tctgggcaag     4140

gatgtgccgg gaggcggcag tacgcgcata acgcgtcacg atgagggggc gggcagtgtg     4200

gtttcgttcg actacaacgc agcagtccag gcgtcggccg ttccccgcct cggccaggta     4260

tacgttctga tttccaacga cggacaaggg gcgcgagccg tcctgctggc ggaggatctg     4320

gcctgggcag gcagcggcag tgccttggat gtgttgaacg aacgattggt gacgttgttt     4380

ccggcgccgg tctga                                                      4395


<210>  20
<211>  1427
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct for ArnT KO

<400>  20
gtgtgccagc tgggaaatca tcgtcgtcga cgacggcagc accgacgata ccgccgagct       60

gatggcgcaa tggtccgcgg tcgagggcat ccgctacgtg cagctgtcgc gcaatttcgg      120

caaggaggcc gccctgacgg ccggcctgga agccgcggac ggcgacgccg tcatctgtct      180

ggacgccgac atgcagcatc cgcccgaact gataggcgac atgctggcgg cctggcgcaa      240

cggcgccgac atggtctacg cggtgcggcg ccagcgcgac gacgagccgt ggttcaagcg      300

tgtcggcgcc cgcgcgttct accgcctgct gagcacggcg cgcggcgtcg aggtgccgcc      360

gcacgcgggc gacttccgcc tgatggaccg ccgcgtggtc gaggccctgg tggcgctgcc      420

cgaacgcacc cgcttcatga aggggctgta tgcctgggtg ggcttcaagt cgcaggccgt      480

gccctacacc ccgcaagcgc gccgccatgg cgccagccac ttcagcgcct ggaagctgtt      540

tcgcctggcc tgcgacggcc tgaccgcctt caccacctgg ccgctgcggc tggtcagcct      600

catcggcgtg ctgttcgcgc tgctgtcgct gtcctacggc ggttacctgg tggccgacta      660

tctcatcagc ggcaacgcgg tgtccggctg gaccaccatc gtcaccgcgc tgctgttctt      720

tgccggcatc aacctgattt cgctgggcgt ggtgggcgag tacgtggccc ggatcttcga      780

cgaagtgaaa ggccggcccc tgttcatcgc ccgtcaacgc cgcgggcgcg ccaagcgcgc      840

ggccaaggcg cgttcccaat gactctcgct acccgatcca tgcaacccca tgcggtttcc      900

cctggccagg ccaggtcctg gcccctgccc gcggcgggct ggctgcttct cgcggtcgga      960

tagctcgagt gacggaacgc ccacaggcgg ctagcgacga atgtcaggaa ggccagcccg     1020

atcaacacca gggccagcag cacgtcatag ggcagcgacg tggcgtgcag cagccaggca     1080

tatgacaact cattgatggc gaaacccagg gctgacacaa ggaagaaacg ccgggcggct     1140

atggtccagg atgccgcaag gtggcggaaa gtgagtctga aatgaccagt gaacgatacg     1200

acgaaggcca acagccagcc caccacgttg gcccccagcg ggggcaggcc ggcccattcc     1260

acgcaggcga ccgcgacggc ccaatgggtg gcggccgcgg cgcatccgac ggcaatgaac     1320

caggcaatct gtcgcagcag gccgcgcatg tcgaagaggt gtctcccttg aggaaccagg     1380

ccggcgatgt ccgttgccgg cgcatcgcgg tgtgcggcga tgatttt                   1427


<210>  21
<211>  2705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct for replacing DNT with LpxD P. aeruginosa

<400>  21
cgccgccagc atttccgagg cgctcggcta cgagcccggc gtcaacggta tccacgcgcc       60

agcggctgcc tgagtccggc ctggcctggc gataaggcgg tgcgtgcagg cgtggctgcg      120

atcgcaccgt aaccgttggt gcggcgtcct gatctccgga gcgttcggct gggagatggc      180

tgcgcatccg atgggccggt tcttgtgtgt ttgctagcct gactaacagg cgacctgggg      240

tcgtttccgg tttccggtca ggggcaaaca aacgtggata aagatgaatc ggcattgcgg      300

caacttgtcg acatggcgct tgtaggctac gacggcgtgg tggaagagtt gctggcgctg      360

ccctcggaag agtccgggga tcttgcgggt ggacgggcca aacgagagaa ggccgagttc      420

gcgctgttta gcgaagcgcc caacggggat gaacccatcg gccaggactg agagctctcc      480

ttgagtgaac tggggggtcg gcgatttcca gtttctcaaa tccggttcgg atgaaccatg      540

catacaacct attgaatctt cacagttagc ccgcgcgcga ttccggatta aggggacagg      600

atgttgcaac ttaccaacaa tggggcggga aaccggcttt tttctgagcc tgaccatagc      660

cagtcctgcc gatttttgat gcaatagcgt caacttccct tcgcggagtt agggggggcg      720

gcaggccggt atgtgctgca ttgctgctct tgtcactcaa atcaacggag attggtacca      780

tgatgagcac tttaagctac actttaggcc agctggccgc ccatgtgggc gccgaggtcc      840

gcggcgacgc cgatctgccc attcaaggtt tagccacgct gcaagaagcc ggcccggcgc      900

agctgagctt tttagcgaac ccccagtacc gcaagtacct cccggagtcg cgcgccggcg      960

ccgtgctgct gaccgccgcg gacgccgacg gcttcgccgg caccgcttta gtcgtggcca     1020

atccctattt agcgtacgcc tctttatcgc acctcttcga tcgcaaaccg aaggccgccg     1080

cgggcatcca cccgaccgcg atcgtggccg ccgatgccga agtggacccg agcgcgagcg     1140

tgggggccta tgccgtcatc gagagcggcg cccgcatcgg cgccggcgtg agcatcggcg     1200

cgcattgcgt gattggcgcg cgcagcgtca ttggcgaagg cggctggctg gcgccgcgcg     1260

tgactttata ccatgacgtg accatcggcg ctcgcgtcag cattcagagc ggcgccgtca     1320

tcgggggcga gggctttggc ttcgccaacg agaagggcgt ctggcagaaa atcgcccaaa     1380

tcggcggcgt caccatcggc gacgacgtgg agattggcgc caacaccacc atcgatcgcg     1440

gcgcgctgtc ggacaccctc atcggcaatg gcgtcaagct ggacaaccag attatgatcg     1500

cgcacaacgt gcagatcggc gaccataccg ccatggccgc ttgtgtgggc attagcggca     1560

gcgccaagat cggccgccac tgcatgctgg ccggcggcgt gggtttagtg ggccatatcg     1620

agatttgcga caacgtgttc gtcaccggca tgaccatggt gacccggagc atcacggagc     1680

cgggctcgta cagcagcggc accgcgatgc aaccggccgc cgagtggaag aagtcggccg     1740

cccgcatccg ccaactggat gacatggcgc gccgtttaca acagctggag aagcgcctcg     1800

ccgccgtgac cagcagcggc gatgccagca gcgacgcctg aaagcttttg aacgaacgat     1860

tggtgacgtt gtttccggcg ccggtctgaa tcgcattccg cgaaacggcg ggcgcgcagg     1920

cctcgcccga tcggccggcc gggccgcgcg atgcgcggcc cggccgcatt gggacgcggc     1980

ctttattgct tttccacgcc cgccttcttg aacagctcgg cccattgcgg cacctgctgc     2040

ttgaccttgg cttgcagcgc ctcgggatta gcctcgttgg tcagcacctc ggcgcccagc     2100

tgcttcatgc gctcctggaa cttgggatcg gccaggccgg cctgcaggct cttgaccagc     2160

ttgtcgacca ccggcttggg cgtgcccttg ggggcccaca tgccgtgcca gatacccact     2220

tcgaaaccct tgtagccgga ctcgtccatg gtgggcaggt cgggcagggt gggcacgcgc     2280

ttgaggctgg tgaccgcgta ggccttcacc ttgccgctgg tgatctgctg cgtggtattg     2340

gtggtctggt cgcacatcag gtcgacctgc ttgcccagca gatcgttcat ggccggcgcc     2400

gtgcccttgt agggaatggt cagcaggttg acgcccagcg cctcgaccag catggtgccg     2460

cacaagtggc tggccgcgcc gatgccggca ttggccagtg aaatcttgtc ggcgttcttc     2520

ttgacgtact cggccagttc cttgatgttg ttgggcggga aatcgccgcg ggcaatgatg     2580

gtcatcggca cgtccaccac caggccgatg ggctcgaagc tggtgtaggg ctcgtagccg     2640

gggttcttgt acagcgaggg cgcggtcgag aagccggcgt gcatgagcag gatcgagtag     2700

ccgtc                                                                 2705


<210>  22
<211>  720
<212>  DNA
<213>  Artificial

<220>
<223>  LpxE F. novicida (optimized for expression in B. pertussis)

<400>  22
atgctgaagc agaccctcca gacgaacttc caaggtttca aggacatctt caagaagccg       60

aagctgcaca accacaagct gccccgctac ctccagctga agtacacctt catcccttta      120

ctgattttag tgatcttcgc gtactacaac ctcgataccc ccgtcgagaa ctacatcaag      180

cacagcatgc cgaacatcgt cggcgtcatc tttggcaaga tcacggacgt cggcaaggcc      240

gagtacattt taatcatctg cggcgtgatc gttttagccc gtttattcac cgacagccag      300

aagctgagcg cgaacacgcg cgcgatgttc gacaaggtct cggcctatgc cggcttcatt      360

ttagccacgg tggccatctc gggcattctg ggccagattt taaagatgat catcgggcgc      420

gcgcgcccca agttcttttt agagtatggc agccactact tccaacactt ccacgccccc      480

ggctacgact ttgccagcat gcccagcggc cactcgatca cggtcggcgc catgttcatc      540

gccttcttct acatcttccc gaagctgcgc tatttctggt atttactcat cgtcgtgttc      600

gccggctcgc gcatcatggt cggctctcac tatccgagcg acgtcatctt cggcgtcgcg      660

ttcggctgct actgcaccgc ctatatctac tactggatgc gcaatcgcga gatcatctaa      720


<210>  23
<211>  735
<212>  DNA
<213>  Artificial

<220>
<223>  LpxE R. leguminosarum (optimized for expression in B. pertussis)

<400>  23
atgcgcgcct tctgggcctc tttagaccgc cgctggcgcc gcagcggcgt gggcatgccg       60

ccgctgcgct ggcaagcatg tttatttgtc actttaaacg cggtgatttt aagcatgctg      120

ctgttcgacg cgccgatcgg cgcgagcaag gcccccgccc cggtgaagca tctgggggaa      180

ctgctgacgg gcttcggcga ctcggcttgg ctgatctaca ccagcatctt attattcttc      240

caaggtcgcg ccggctacaa gctgctgaaa acggcccgct cgaaggccca agccttatat      300

gtcagctgga tcggcgccta tctgttcttc accgtggtct tcagcggttt actggccaat      360

ctgctgaagc gcgccatcgg ccgcgcccgc ccggaccatt tccacgacta cggcatgttc      420

agcttcgccc cgttctcggg ccacagcgcc ttcgagagct tcccgagcgg ccatagcacc      480

accgtgggcg cctttttcgc cgcctttgct ttactgttcc cgcgctaccg cgtggcgttc      540

atcgcttgtg ccatctggct gggcatgacc cgcgtgatgg tgggcgccca ttacccgagc      600

gatgtcatcg ccggtttagc ctttggcgct tggttctctt tactgaccgc catcgtgttc      660

gcgcgctgtg gtttactgtt caagctggcc ccggacggct ggccgctcgc gaagcgttta      720

ttccgcaccg cgtaa                                                       735


<210>  24
<211>  669
<212>  DNA
<213>  Artificial

<220>
<223>  LpxF F. novicida (optimized for expression in B. pertussis)

<400>  24
atggcccgct tccacatcat tttagggctg gtggtgtgct tcttcgcttg gatcttcttt       60

ttaatcttcc cgaatttaga catccagttt gccggccact tctacaactc gagcgcccat      120

cagttcatcg gcggctacga cgggtttctg ggctttttac actggttcgc gcgcttcttc      180

cctatcttct tcagcatcat cgtgatttta tttttactgg gctctttatt cattgacaag      240

ttcaagatca agtaccggaa ggcgatcttt ttcatcgccg tgtgtttatg gatcggcccc      300

ggtttagtcg tcaactacgt cttcaaggac cactggggcc gcccgcgccc ggtcatggtc      360

gagcagttca acggcgacaa gatcttccag ccgcccttcg tgatctcgag ccagtgcgac      420

aagaactgca gcttcgtctg cggcgacgcc agcatgggct tctggctgtt cgcgttcatg      480

ccgctgctcg ccacccgcaa gaagaagctg gtggccttca tcgccgccgt ggtcgccggc      540

ggcggtttag gcctcatgcg catgagccaa ggcggccact tctttagcga cgtcgtgttt      600

tgcggcatct tcgtgtacat cagcacttgg gtggtctacg ccctcatgta ccgcaagaag      660

gaatactaa                                                              669


