                         SEQUENCE LISTING

<110>  The Trustees Of The University Of Pennsylvania
       Pure, Ellen
       Todd, Leslie
 
<120>  MONOCLONAL ANTIBODY AGAINST CANINE FIBROBLAST ACTIVATION PROTEIN 
       THAT CROSS-REACTS WITH MOUSE AND HUMAN FIBROBLAST ACTIVATION 
       PROTEIN (FAP)

<130>  046483-7274WO1(02348)

<150>  62/904,272
<151>  2019-09-23

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 HCDR1

<400>  1

Tyr Thr Ile Thr Ser Tyr Ser Leu His 
1               5                   


<210>  2
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 HCDR2

<400>  2

Glu Ile Asn Pro Ala Asn Gly Asp His Asn Phe Ser Glu Lys Phe Glu 
1               5                   10                  15      


Ile Lys 
        


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 HCDR3

<400>  3

Leu Asp Asp Ser Arg Phe His Trp Tyr Phe Asp Val 
1               5                   10          


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 LCDR1

<400>  4

Thr Ala Ser Ser Ser Val Ser Tyr Met Tyr 
1               5                   10  


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 LCDR2

<400>  5

Leu Thr Ser Asn Leu Ala 
1               5       


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 LCDR3

<400>  6

Gln Gln Trp Ser Gly Tyr Pro Pro Ile Thr 
1               5                   10  


<210>  7
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 VH

<400>  7

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Ile Thr Ser Tyr 
            20                  25                  30          


Ser Leu His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Ala Asn Gly Asp His Asn Phe Ser Glu Lys Phe 
    50                  55                  60                  


Glu Ile Lys Ala Thr Leu Thr Val Asp Ser Ser Ser Asn Thr Ala Phe 
65                  70                  75                  80  


Met Gln Leu Ser Arg Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Leu Asp Asp Ser Arg Phe His Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  8
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4G5 VH

<400>  8
caggtccaac tgcagcagcc tggggctgaa ctggtaaagc ctggggcttc agtgaagttg       60

tcctgcaagg cgtctggcta caccatcacc agctactctc tgcactgggt gaagcagagg      120

cctggacaag gccttgagtg gattggagag attaatcctg ccaatggtga tcataacttc      180

agtgagaagt tcgagatcaa ggccacactg actgtagaca gctcctccaa cacagcattc      240

atgcaactca gcaggctgac atctgaggac tctgcggtct attactgtac aagattggac      300

gatagtaggt tccactggta cttcgatgtc tggggcgcag ggaccacggt caccgtctcc      360

tca                                                                    363


<210>  9
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 VL

<400>  9

Gln Ile Val Leu Thr Gln Ser Pro Ala Leu Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Thr Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Arg Ser Ser Pro Lys Pro Trp Ile Phe 
        35                  40                  45              


Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Arg 
    50                  55                  60                  


Gly Ser Gly Thr Ser Phe Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Gly Tyr Pro Pro Ile 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  10
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4G5 VL

<400>  10
caaattgttc tcacccagtc tccagcgctc atgtctgctt ctccagggga gaaggtcacc       60

atgacctgca ctgccagctc aagtgttagt tacatgtact ggtaccagca gaagccacga      120

tcctccccca aaccctggat ttttctcacc tccaacctgg cttctggagt ccctgctcgc      180

ttcagtggcc gtgggtctgg gacctctttc tctctcacaa tcagcagcat ggaggctgaa      240

gatgctgcca cttattactg ccagcagtgg agtggttacc cacccatcac attcggctcg      300

gggacaaagt tggaaataaa a                                                321


<210>  11
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 scFv VLVH

<400>  11

Gln Ile Val Leu Thr Gln Ser Pro Ala Leu Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Thr Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Arg Ser Ser Pro Lys Pro Trp Ile Phe 
        35                  40                  45              


Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Arg 
    50                  55                  60                  


Gly Ser Gly Thr Ser Phe Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Gly Tyr Pro Pro Ile 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln 
        115                 120                 125             


Pro Gly Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Leu Ser Cys 
    130                 135                 140                 


Lys Ala Ser Gly Tyr Thr Ile Thr Ser Tyr Ser Leu His Trp Val Lys 
145                 150                 155                 160 


Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Glu Ile Asn Pro Ala 
                165                 170                 175     


Asn Gly Asp His Asn Phe Ser Glu Lys Phe Glu Ile Lys Ala Thr Leu 
            180                 185                 190         


Thr Val Asp Ser Ser Ser Asn Thr Ala Phe Met Gln Leu Ser Arg Leu 
        195                 200                 205             


Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Thr Arg Leu Asp Asp Ser 
    210                 215                 220                 


Arg Phe His Trp Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr 
225                 230                 235                 240 


Val Ser Ser 
            


<210>  12
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4G5 scFv VLVH

<400>  12
caaattgttc tcacccagtc tccagcgctc atgtctgctt ctccagggga gaaggtcacc       60

atgacctgca ctgccagctc aagtgttagt tacatgtact ggtaccagca gaagccacga      120

tcctccccca aaccctggat ttttctcacc tccaacctgg cttctggagt ccctgctcgc      180

ttcagtggcc gtgggtctgg gacctctttc tctctcacaa tcagcagcat ggaggctgaa      240

gatgctgcca cttattactg ccagcagtgg agtggttacc cacccatcac attcggctcg      300

gggacaaagt tggaaataaa aggtggaggt ggcagcggag gaggtgggtc cggcggtgga      360

ggaagccagg tccaactgca gcagcctggg gctgaactgg taaagcctgg ggcttcagtg      420

aagttgtcct gcaaggcgtc tggctacacc atcaccagct actctctgca ctgggtgaag      480

cagaggcctg gacaaggcct tgagtggatt ggagagatta atcctgccaa tggtgatcat      540

aacttcagtg agaagttcga gatcaaggcc acactgactg tagacagctc ctccaacaca      600

gcattcatgc aactcagcag gctgacatct gaggactctg cggtctatta ctgtacaaga      660

ttggacgata gtaggttcca ctggtacttc gatgtctggg gcgcagggac cacggtcacc      720

gtctcctca                                                              729


<210>  13
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 scFv VHVL

<400>  13

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Ile Thr Ser Tyr 
            20                  25                  30          


Ser Leu His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Ala Asn Gly Asp His Asn Phe Ser Glu Lys Phe 
    50                  55                  60                  


Glu Ile Lys Ala Thr Leu Thr Val Asp Ser Ser Ser Asn Thr Ala Phe 
65                  70                  75                  80  


Met Gln Leu Ser Arg Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Leu Asp Asp Ser Arg Phe His Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln Ser Pro 
    130                 135                 140                 


Ala Leu Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Thr 
145                 150                 155                 160 


Ala Ser Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Arg 
                165                 170                 175     


Ser Ser Pro Lys Pro Trp Ile Phe Leu Thr Ser Asn Leu Ala Ser Gly 
            180                 185                 190         


Val Pro Ala Arg Phe Ser Gly Arg Gly Ser Gly Thr Ser Phe Ser Leu 
        195                 200                 205             


Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Trp Ser Gly Tyr Pro Pro Ile Thr Phe Gly Ser Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys 
            


<210>  14
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4G5 scFv VHVL

<400>  14
caggtccaac tgcagcagcc tggggctgaa ctggtaaagc ctggggcttc agtgaagttg       60

tcctgcaagg cgtctggcta caccatcacc agctactctc tgcactgggt gaagcagagg      120

cctggacaag gccttgagtg gattggagag attaatcctg ccaatggtga tcataacttc      180

agtgagaagt tcgagatcaa ggccacactg actgtagaca gctcctccaa cacagcattc      240

atgcaactca gcaggctgac atctgaggac tctgcggtct attactgtac aagattggac      300

gatagtaggt tccactggta cttcgatgtc tggggcgcag ggaccacggt caccgtctcc      360

tcaggtggag gtggcagcgg aggaggtggg tccggcggtg gaggaagcca aattgttctc      420

acccagtctc cagcgctcat gtctgcttct ccaggggaga aggtcaccat gacctgcact      480

gccagctcaa gtgttagtta catgtactgg taccagcaga agccacgatc ctcccccaaa      540

ccctggattt ttctcacctc caacctggct tctggagtcc ctgctcgctt cagtggccgt      600

gggtctggga cctctttctc tctcacaatc agcagcatgg aggctgaaga tgctgccact      660

tattactgcc agcagtggag tggttaccca cccatcacat tcggctcggg gacaaagttg      720

gaaataaaa                                                              729


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  15

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  16
<211>  2283
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full Length Canine FAP

<400>  16
atgaagacgt ggttaaaaat tgtatttgga gttgccacct ctgctgtgct tgctttattg       60

gtgatgtgca ttgtcttacg tccttcaaga gttcatgact ccgaaggagg tacaacaaga      120

gcactcacac tggaggatat tttaaatggg acatttacct ataaaacatt ttttccaaac      180

tggatttcag gacaagaata tcttcatcag tctacagata atgatatagt atattacaat      240

attgaaacag gagaatcata taccattttg agtaatgcca ccatgaaaag tgtgaatgct      300

tcaaattatg gcttatcacc tgatcgtcaa tttgcatatc tagaaagtga ttattcaaag      360

ctttggagat actcttacac tgcaacatat cacatctata acctcaataa tggagagttt      420

ataagaagaa atgagcttcc tcgtccaatt cagtatttat gctggtcgcc tgttgggagt      480

aaattagcat atgtctatca aaacaatatc tatttgaaac aaagaccaga agacccacct      540

tttcaaataa catataatgg aagagaaaat aaaatattca atggaatccc agactgggta      600

tatgaagagg aaatgcttgc tacaaaacat gctctctggt ggtctcctaa tggaaaattt      660

ttggcatatg cagaatttaa tgatacagag ataccagtta ttgcctattc ctattatggt      720

gatgaacaat atcctagaac aataaatatt ccatacccaa aggctggagc taagaaccct      780

gttgttcgga tctttattat cgataccact tatcctcagc agacaggtcc cagagaagtg      840

ccagttccag caatgatagc atcaagtgat tattatttca gttggctcac atgggttact      900

gatgaacgag tatgtttgca gtggctaaaa agaatccaga acgtttcagt tctgtccata      960

tgtgatttca gggaaggctg gcagacatgg gattgtccaa aggcccagga acatatagaa     1020

gaaagcagaa ctggatgggc tggtggattc tttgtttcaa caccagtttt cagctatgat     1080

gccatttcat actacaaaat atttagcgac aaggatggct acaaacatat tcactatatc     1140

aaagacactg tggaaaatgc tattcaaatt acaagtggca agtgggaggc cataaatata     1200

ttcagagtaa cacaggattc actgttttat tctagcaatg aatttgaaga ctacccagga     1260

agaagaaata tctatagaat tagcattgga agctctcctc caagcaaaaa gtgcattact     1320

tgccatctaa ggaaagaaag gtgccaatat tacacagcaa gtttcagtga ctacgccaag     1380

tactatgcac ttatctgcta tggcccaggc ctccccattt ccacccttca tgacggccac     1440

actgatcaag aaattaaaat cctggaagaa aacaaagaat tggaaaatgc tttgaaaaat     1500

atccagctgc ctaaagagga aattaagaaa cttgaagtgg atgatattac tttatggtac     1560

aagatgatgc ttcctccccg gtttgacaga tcaaagaagt atcccttgct aattcaagtg     1620

tatggtggtc cctgcagtca gagcgtaaag tctgtattca gtattaattg gatttcttat     1680

cttgcaagta aggaagggat agtcattgcc ttggtggatg gccgaggaac agcttaccaa     1740

ggtgacaaac tcctgtatgc agtatatcga aagctgggtg tttatgaagt tgaggaccag     1800

atcacagccg tcagaaaatt catagaaatg ggtttcattg atgaaaaaag aatagccata     1860

tggggctggt cctatggagg ctatgtttca tcactggccc ttgcttcagg aactggtctt     1920

ttcaaatgtg ggatagcagt ggctcctgtc tccagctggg aatattacgc atctatctac     1980

acagaacgat tcatgggcct cccaacaaag aacgataatc tcgagcacta caaaaattca     2040

actgtgatgg caagagcaga atatttcaga aatgtagact atcttctcat ccacggaaca     2100

gcagatgata atgtgcactt tcaaaactca gcacagattg ctaaagctct ggttaatgca     2160

caagtggatt tccaggcaat gtggtactct gaccagaacc atggcatacc cggcctgtcc     2220

tcgaagcact tatatacccg catgacccac ttcctaaagc agtgtttttc tttgtccgac     2280

tga                                                                   2283


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 HCDR1

<400>  17

Gly Tyr Thr Ile Thr Ser Tyr Ser Leu His 
1               5                   10  


<210>  18
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 HCDR2

<400>  18

Glu Ile Asn Pro Ala Asn Gly Asp His Asn Phe Ser Glu Lys Phe Glu 
1               5                   10                  15      


Ile Lys Ala Thr 
            20  


<210>  19
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 HCDR3

<400>  19

Thr Arg Leu Asp Asp Ser Arg Phe His Trp Tyr Phe Asp Val 
1               5                   10                  


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4G5 LCDR2

<400>  20

Leu Thr Ser Asn Leu Ala Ser 
1               5           


<210>  21
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n represents an integer of at least 1

<400>  21

Gly Ser Gly Gly Ser 
1               5   


<210>  22
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  repeat n times, where n represents an integer of at least 1

<400>  22

Gly Gly Gly Ser 
1               


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n represents an integer of at least 1

<400>  23

Gly Gly Gly Gly Ser 
1               5   


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  24

Gly Gly Ser Gly 
1               


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  25

Gly Gly Ser Gly Gly 
1               5   


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  26

Gly Ser Gly Ser Gly 
1               5   


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  27

Gly Ser Gly Gly Gly 
1               5   


<210>  28
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  28

Gly Gly Gly Ser Gly 
1               5   


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  29

Gly Ser Ser Ser Gly 
1               5   


<210>  30
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  30

Gly Gly Gly Gly Ser 
1               5   


<210>  31
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  31

Gly Gly Thr Gly Gly Cys Gly Gly Thr Gly Gly Cys Thr Cys Gly Gly 
1               5                   10                  15      


Gly Cys Gly Gly Thr Gly Gly Thr Gly Gly Gly Thr Cys Gly Gly Gly 
            20                  25                  30          


Thr Gly Gly Cys Gly Gly Cys Gly Gly Ala Thr Cys Thr 
        35                  40                  45  


<210>  32
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  32

Arg Xaa Lys Arg 
1               


<210>  33
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  33

Arg Xaa Arg Arg 
1               


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  34

Xaa Arg Xaa Xaa Arg 
1               5   


<210>  35
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  35

Arg Xaa Xaa Arg 
1               


<210>  36
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site

<400>  36

Arg Gln Lys Arg 
1               


<210>  37
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  37
atgtagacgt ggttaaaaat tg                                                22


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  38
cgtcatcttc agtcggacaa                                                   20


