                         SEQUENCE LISTING

<110>  BASF SE
<110>  Rijksuniversiteit te Groningen

<120>  Method for the production of beta-alanine or salts thereof

<130> 190818WO01

<150> EP19199764.2
<151> 2019-09-26

<160>  55

<170>  According Wipo Std 25


<210> 1
<211> 1407
<212> DNA
<213> Bacillus sp.

<220>
<223> Bacillus spec. YM55-1: wild type AspB

<400> 1
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaac gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atgcctggta aagtgaatcc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 2
<211> 468
<212> PRT
<213> Bacillus sp.

<220>
<223> Bacillus spec. YM55-1: wild type AspB

<400> 2
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Thr His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Met Pro Gly Lys Val Asn Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 3
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A1

<400> 3
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 4
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A1

<400> 4
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Met Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 5
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A2

<400> 5
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atgcctggtt ttgtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 6
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A2

<400> 6
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Met Pro Gly Phe Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 7
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A3

<400> 7
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta tggtggcgcc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 8
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A3

<400> 8
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Met Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 9
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A4

<400> 9
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtg tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 10
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A4

<400> 10
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Val Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 11
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A5

<400> 11
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta ttgtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 12
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A5

<400> 12
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Ile Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 13
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A6

<400> 13
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtt gtgtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 14
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A6

<400> 14
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Cys Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 15
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A7

<400> 15
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtc tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 16
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A7

<400> 16
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Leu Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 17
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A9

<400> 17
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atgcctggtc cggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 18
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A9

<400> 18
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Met Pro Gly Pro Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 19
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A10_

<400> 19
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtc tggtggcgcc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 20
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A10

<400> 20
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Leu Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 21
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A11

<400> 21
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta ttgtggcgcc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 22
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A11

<400> 22
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Ile Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 23
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A12

<400> 23
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtg tggtggcgcc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 24
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A12

<400> 24
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Val Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 25
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A13

<400> 25
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtt gcgtggcgcc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 26
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A13

<400> 26
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Cys Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 27
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A14

<400> 27
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtt ttgtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 28
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A14

<400> 28
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Phe Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 29
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A15

<400> 29
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaat tcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtg cggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 30
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A15

<400> 30
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Ile His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Ala Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 31
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A16

<400> 31
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtt gcgtggcccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 32
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A16

<400> 32
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Cys Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 33
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-A17

<400> 33
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta ttgtggcccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 34
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-A17

<400> 34
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Ile Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 35
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-Aint

<400> 35
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtc tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 36
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-Aint

<400> 36
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Leu Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 37
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-B1

<400> 37
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta ttgtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 38
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-B1

<400> 38
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Ile Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 39
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-B2

<400> 39
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtt gtgtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 40
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-B2

<400> 40
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Cys Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 41
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-B5

<400> 41
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta tggtggcccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 42
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-B5

<400> 42
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Met Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 43
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-B7

<400> 43
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtg tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 44
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-B7

<400> 44
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Val Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 45
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-B10

<400> 45
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagagt gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 46
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-B10

<400> 46
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Val His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Met Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 47
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-B19

<400> 47
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagatg ccacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtc tggtggcgcc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 48
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-B19

<400> 48
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Cys His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Leu Val Ala Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 49
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-N5

<400> 49
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagaac gcacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta atgtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 50
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-N5

<400> 50
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Thr His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Asn Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 51
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-P1

<400> 51
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagatg ccacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggtc tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 52
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-P1

<400> 52
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Cys His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Leu Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 53
<211> 1407
<212> DNA
<213> Artificial Sequence

<220>
<223> mutant: AspB-Vogel

<400> 53
atgaataccg atgttcgtat tgagaaagac tttttaggag aaaaggagat tccgaaagac     60
gcttattatg gcgtacaaac aattcgggca acggaaaatt ttccaattac aggttatcgt    120
attcatccag aattaattaa atcactaggg attgtaaaaa aatcagccgc attagcaaac    180
atggaagttg gcttactcga taaagaagtt gggcaatata tcgtaaaagc tgctgacgaa    240
gtgattgaag gaaaatggaa tgatcaattt attgttgacc caattcaagg cggggcagga    300
acttccatta atatgaatgc aaatgaagtg attgctaacc gcgcattaga attaatggga    360
gaggaaaaag gaaactattc aaaaattagt ccaaactccc atgtaaatat gtctcaatca    420
acaaacgatg ctttccctac tgcaacgcat attgctgtgt taagtttatt aaatcaatta    480
attgaaacta caaaatacat gcaacaagaa ttcatgaaaa aagcagatga attcgctggc    540
gttattaaaa tgggaagatg ccacttgcaa gacgctgttc ctattttatt aggacaagag    600
tttgaagcat atgctcgtgt aattgcccgc gatattgaac gtattgccaa tacgagaaac    660
aatttatacg acatcaacat gggtgcaaca gcagtcggca ctggcttaaa tgcagatcct    720
gaatatataa gcatcgtaac agaacattta gcaaaattca gcggacatcc attaagaagt    780
gcacaacatt tagtggacgc aactcaaaat acagactgct atacagaagt ttcttctgca    840
ttaaaagttt gcatgatcaa catgtctaaa attgccaatg atttacgctt aatggcatct    900
ggaccacgcg caggcttatc agaaatcgtt cttcctgctc gacaacctgg atcttctatc    960
atccctggta tggtgtgccc tgttatgcca gaagtgatga accaagtggc attccaagtg   1020
ttcggtaatg atttaacaat tacatctgct tctgaagcag gccaatttga attaaatgtg   1080
atggaacctg tgttattctt caatttaatt caatcgattt cgattatgac taatgtcttt   1140
aaatccttta cagaaaactg cttaaaaggt attaaggcaa atgaagaacg catgaaagaa   1200
tatgttgaga aaagcattgg aatcattact gcaattaacc cacatgtagg ctatgaaaca   1260
gctgcaaaat tagcacgtga agcatatctt acaggggaat ccatccgtga actttgcatt   1320
aagtatggcg tattaacaga agaacagtta aatgaaatct taaatccata tgaaatgaca   1380
catccgggaa ttgctggaag aaaataa                                       1407

<210> 54
<211> 468
<212> PRT
<213> Artificial Sequence

<220>
<223> mutant: AspB-Vogel

<400> 54
Met Asn Thr Asp Val Arg Ile Glu Lys Asp Phe Leu Gly Glu Lys Glu
1               5                   10                  15
Ile Pro Lys Asp Ala Tyr Tyr Gly Val Gln Thr Ile Arg Ala Thr Glu
            20                  25                  30
Asn Phe Pro Ile Thr Gly Tyr Arg Ile His Pro Glu Leu Ile Lys Ser
        35                  40                  45
Leu Gly Ile Val Lys Lys Ser Ala Ala Leu Ala Asn Met Glu Val Gly
    50                  55                  60
Leu Leu Asp Lys Glu Val Gly Gln Tyr Ile Val Lys Ala Ala Asp Glu
65                  70                  75                  80
Val Ile Glu Gly Lys Trp Asn Asp Gln Phe Ile Val Asp Pro Ile Gln
                85                  90                  95
Gly Gly Ala Gly Thr Ser Ile Asn Met Asn Ala Asn Glu Val Ile Ala
            100                 105                 110
Asn Arg Ala Leu Glu Leu Met Gly Glu Glu Lys Gly Asn Tyr Ser Lys
        115                 120                 125
Ile Ser Pro Asn Ser His Val Asn Met Ser Gln Ser Thr Asn Asp Ala
    130                 135                 140
Phe Pro Thr Ala Thr His Ile Ala Val Leu Ser Leu Leu Asn Gln Leu
145                 150                 155                 160
Ile Glu Thr Thr Lys Tyr Met Gln Gln Glu Phe Met Lys Lys Ala Asp
                165                 170                 175
Glu Phe Ala Gly Val Ile Lys Met Gly Arg Cys His Leu Gln Asp Ala
            180                 185                 190
Val Pro Ile Leu Leu Gly Gln Glu Phe Glu Ala Tyr Ala Arg Val Ile
        195                 200                 205
Ala Arg Asp Ile Glu Arg Ile Ala Asn Thr Arg Asn Asn Leu Tyr Asp
    210                 215                 220
Ile Asn Met Gly Ala Thr Ala Val Gly Thr Gly Leu Asn Ala Asp Pro
225                 230                 235                 240
Glu Tyr Ile Ser Ile Val Thr Glu His Leu Ala Lys Phe Ser Gly His
                245                 250                 255
Pro Leu Arg Ser Ala Gln His Leu Val Asp Ala Thr Gln Asn Thr Asp
            260                 265                 270
Cys Tyr Thr Glu Val Ser Ser Ala Leu Lys Val Cys Met Ile Asn Met
        275                 280                 285
Ser Lys Ile Ala Asn Asp Leu Arg Leu Met Ala Ser Gly Pro Arg Ala
    290                 295                 300
Gly Leu Ser Glu Ile Val Leu Pro Ala Arg Gln Pro Gly Ser Ser Ile
305                 310                 315                 320
Ile Pro Gly Met Val Cys Pro Val Met Pro Glu Val Met Asn Gln Val
                325                 330                 335
Ala Phe Gln Val Phe Gly Asn Asp Leu Thr Ile Thr Ser Ala Ser Glu
            340                 345                 350
Ala Gly Gln Phe Glu Leu Asn Val Met Glu Pro Val Leu Phe Phe Asn
        355                 360                 365
Leu Ile Gln Ser Ile Ser Ile Met Thr Asn Val Phe Lys Ser Phe Thr
    370                 375                 380
Glu Asn Cys Leu Lys Gly Ile Lys Ala Asn Glu Glu Arg Met Lys Glu
385                 390                 395                 400
Tyr Val Glu Lys Ser Ile Gly Ile Ile Thr Ala Ile Asn Pro His Val
                405                 410                 415
Gly Tyr Glu Thr Ala Ala Lys Leu Ala Arg Glu Ala Tyr Leu Thr Gly
            420                 425                 430
Glu Ser Ile Arg Glu Leu Cys Ile Lys Tyr Gly Val Leu Thr Glu Glu
        435                 440                 445
Gln Leu Asn Glu Ile Leu Asn Pro Tyr Glu Met Thr His Pro Gly Ile
    450                 455                 460
Ala Gly Arg Lys
465

<210> 55
<211> 6751
<212> DNA
<213> Artificial Sequence

<220>
<223> Plasmid pET21_AspB_WT

<400> 55
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg     60
cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc    120
ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg    180
gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc    240
acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt    300
ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc    360
ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta    420
acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt    480
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta    540
tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat    600
gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt    660
ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg    720
agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga    780
agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg    840
tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt    900
tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg    960
cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg   1020
aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga   1080
tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc   1140
tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc   1200
ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc   1260
ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg   1320
cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac   1380
gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc   1440
actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt   1500
aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac   1560
caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa   1620
aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc   1680
accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt   1740
aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg   1800
ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc   1860
agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt   1920
accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga   1980
gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct   2040
tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg   2100
cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca   2160
cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa   2220
cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt   2280
ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga   2340
taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga   2400
gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg   2460
tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat   2520
cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct   2580
gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct   2640
gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct   2700
catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt   2760
tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg   2820
ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa   2880
tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc   2940
ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa   3000
aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta   3060
gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg   3120
tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag   3180
acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac   3240
cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca   3300
cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg   3360
gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc   3420
cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg   3480
gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca   3540
tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag   3600
atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt   3660
tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag   3720
gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc   3780
tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc   3840
cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct   3900
tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta   3960
atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg   4020
atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct   4080
tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga   4140
cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc   4200
aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg   4260
ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct   4320
tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt   4380
tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc   4440
gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc   4500
gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc   4560
gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact   4620
ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga   4680
taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc   4740
ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg   4800
atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag   4860
tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc   4920
gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat   4980
gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc   5040
aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat   5100
ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc   5160
cctctagaaa taattttgtt taactttaag aaggagatat acatatgaat accgatgttc   5220
gtattgagaa agacttttta ggagaaaagg agattccgaa agacgcttat tatggcgtac   5280
aaacaattcg ggcaacggaa aattttccaa ttacaggtta tcgtattcat ccagaattaa   5340
ttaaatcact agggattgta aaaaaatcag ccgcattagc aaacatggaa gttggcttac   5400
tcgataaaga agttgggcaa tatatcgtaa aagctgctga cgaagtgatt gaaggaaaat   5460
ggaatgatca atttattgtt gacccaattc aaggcggggc aggaacttcc attaatatga   5520
atgcaaatga agtgattgct aaccgcgcat tagaattaat gggagaggaa aaaggaaact   5580
attcaaaaat tagtccaaac tcccatgtaa atatgtctca atcaacaaac gatgctttcc   5640
ctactgcaac gcatattgct gtgttaagtt tattaaatca attaattgaa actacaaaat   5700
acatgcaaca agaattcatg aaaaaagcag atgaattcgc tggcgttatt aaaatgggaa   5760
gaacgcactt gcaagacgct gttcctattt tattaggaca agagtttgaa gcatatgctc   5820
gtgtaattgc ccgcgatatt gaacgtattg ccaatacgag aaacaattta tacgacatca   5880
acatgggtgc aacagcagtc ggcactggct taaatgcaga tcctgaatat ataagcatcg   5940
taacagaaca tttagcaaaa ttcagcggac atccattaag aagtgcacaa catttagtgg   6000
acgcaactca aaatacagac tgctatacag aagtttcttc tgcattaaaa gtttgcatga   6060
tcaacatgtc taaaattgcc aatgatttac gcttaatggc atctggacca cgcgcaggct   6120
tatcagaaat cgttcttcct gctcgacaac ctggatcttc tatcatgcct ggtaaagtga   6180
atcctgttat gccagaagtg atgaaccaag tggcattcca agtgttcggt aatgatttaa   6240
caattacatc tgcttctgaa gcaggccaat ttgaattaaa tgtgatggaa cctgtgttat   6300
tcttcaattt aattcaatcg atttcgatta tgactaatgt ctttaaatcc tttacagaaa   6360
actgcttaaa aggtattaag gcaaatgaag aacgcatgaa agaatatgtt gagaaaagca   6420
ttggaatcat tactgcaatt aacccacatg taggctatga aacagctgca aaattagcac   6480
gtgaagcata tcttacaggg gaatccatcc gtgaactttg cattaagtat ggcgtattaa   6540
cagaagaaca gttaaatgaa atcttaaatc catatgaaat gacacatccg ggaattgctg   6600
gaagaaaata atgagatccg gctgctaaca aagcaccgaa aggaagctga gttggctgct   6660
gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt   6720
tttttgctga aaggaggaac tatatccgga t                                  6751
