                         SEQUENCE LISTING

<110>  NORTHWESTERN UNIVERSITY
 
<120>  COST EFFECTIVE CULTURE MEDIA AND PROTOCOL FOR HUMAN INDUCED 
       PLURIPOTENT STEM CELLS

<130>  47460-109

<150>  62/902,561
<151>  2019-09-19

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1

Met Phe Asn Leu Pro Pro Gly Asn Tyr Lys Lys Pro Lys Leu Leu Tyr 
1               5                   10                  15      


Cys Ser Asn Gly Gly His Phe Leu Arg Ile Leu Pro Asp Gly Thr Val 
            20                  25                  30          


Asp Gly Thr Arg Asp Arg Ser Asp Gln His Ile Gln Leu Gln Leu Ser 
        35                  40                  45              


Ala Glu Ser Val Gly Glu Val Tyr Ile Lys Ser Thr Glu Thr Gly Gln 
    50                  55                  60                  


Tyr Leu Ala Met Asp Thr Asp Gly Leu Leu Tyr Gly Ser Gln Thr Pro 
65                  70                  75                  80  


Asn Glu Glu Cys Leu Phe Leu Glu Arg Leu Glu Glu Asn His Tyr Asn 
                85                  90                  95      


Thr Tyr Ile Ser Lys Lys His Ala Glu Lys Asn Trp Phe Val Gly Leu 
            100                 105                 110         


Lys Lys Asn Gly Ser Cys Lys Arg Gly Pro Arg Thr His Tyr Gly Gln 
        115                 120                 125             


Lys Ala Ile Leu Phe Leu Pro Leu Pro Val Ser Ser Asp 
    130                 135                 140     


<210>  2
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2

Met Phe Asn Leu Pro Pro Gly Asn Tyr Lys Lys Pro Lys Leu Leu Tyr 
1               5                   10                  15      


Cys Ser Asn Gly Gly His Phe Leu Arg Ile Leu Pro Asp Gly Thr Val 
            20                  25                  30          


Asp Gly Thr Arg Asp Arg Ser Asp Pro His Ile Gln Leu Gln Leu Ile 
        35                  40                  45              


Ala Glu Ser Val Gly Glu Val Tyr Ile Lys Ser Thr Glu Thr Gly Gln 
    50                  55                  60                  


Tyr Leu Ala Met Asp Thr Asp Gly Leu Leu Tyr Gly Ser Gln Thr Pro 
65                  70                  75                  80  


Asn Glu Glu Cys Leu Phe Leu Glu Arg Leu Glu Glu Asn Gly Tyr Asn 
                85                  90                  95      


Thr Tyr Ile Ser Lys Lys His Ala Glu Lys Asn Trp Phe Val Gly Leu 
            100                 105                 110         


Asn Lys Asn Gly Ser Cys Lys Arg Gly Pro Arg Thr His Tyr Gly Gln 
        115                 120                 125             


Lys Ala Ile Leu Phe Leu Pro Leu Pro Val Ser Ser Asp 
    130                 135                 140     


<210>  3
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3

Met Ala Ala Gly Ser Ile Thr Thr Leu Pro Ala Leu Pro Glu Asp Gly 
1               5                   10                  15      


Gly Ser Gly Ala Phe Pro Pro Gly His Phe Lys Asp Pro Lys Arg Leu 
            20                  25                  30          


Tyr Cys Lys Asn Gly Gly Phe Phe Leu Arg Ile His Pro Asp Gly Arg 
        35                  40                  45              


Val Asp Gly Val Arg Glu Lys Ser Asp Pro His Ile Lys Leu Gln Leu 
    50                  55                  60                  


Gln Ala Glu Glu Arg Gly Val Val Ser Ile Lys Gly Val Cys Ala Asn 
65                  70                  75                  80  


Arg Tyr Leu Ala Met Lys Glu Asp Gly Arg Leu Leu Ala Ser Lys Cys 
                85                  90                  95      


Val Thr Asp Glu Cys Phe Phe Phe Glu Arg Leu Glu Ser Asn Asn Tyr 
            100                 105                 110         


Asn Thr Tyr Arg Ser Arg Lys Tyr Thr Ser Trp Tyr Val Ala Leu Lys 
        115                 120                 125             


Arg Thr Gly Gln Tyr Lys Leu Gly Ser Lys Thr Gly Pro Gly Gln Lys 
    130                 135                 140                 


Ala Ile Leu Phe Leu Pro Met Ser Ala Lys Ser 
145                 150                 155 


<210>  4
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4

Met Ala Ala Gly Ser Ile Thr Thr Leu Pro Ala Leu Pro Glu Asp Gly 
1               5                   10                  15      


Gly Ser Gly Ala Phe Pro Pro Gly His Phe Lys Asp Pro Lys Arg Leu 
            20                  25                  30          


Tyr Cys Lys Asn Gly Gly Phe Phe Leu Arg Ile His Pro Asp Gly Arg 
        35                  40                  45              


Val Asp Gly Val Arg Glu Lys Ser Asp Pro His Ile Lys Leu Gln Leu 
    50                  55                  60                  


Gln Ala Glu Glu Arg Gly Val Val Ser Ile Lys Gly Val Cys Ala Asn 
65                  70                  75                  80  


Arg Tyr Leu Ala Met Lys Glu Asp Gly Arg Leu Leu Ala Ser Lys Cys 
                85                  90                  95      


Val Thr Asp Glu Cys Phe Phe Phe Glu Arg Leu Glu Ser Asn Asn Tyr 
            100                 105                 110         


Asn Thr Tyr Arg Ser Arg Lys Tyr Thr Ser Trp Tyr Val Ala Leu Asn 
        115                 120                 125             


Arg Thr Gly Gln Tyr Lys Leu Gly Ser Lys Thr Gly Pro Gly Gln Lys 
    130                 135                 140                 


Ala Ile Leu Phe Leu Pro Met Ser Ala Lys Ser 
145                 150                 155 


<210>  5
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5

Met Ala Ala Gly Ser Ile Thr Thr Leu Pro Ala Leu Pro Glu Asp Gly 
1               5                   10                  15      


Gly Ser Gly Ala Phe Pro Pro Gly His Phe Lys Asp Pro Lys Leu Leu 
            20                  25                  30          


Tyr Cys Lys Asn Gly Gly Phe Phe Leu Arg Ile His Pro Asp Gly Arg 
        35                  40                  45              


Val Asp Gly Thr Arg Asp Lys Ser Asp Pro Phe Ile Lys Leu Gln Leu 
    50                  55                  60                  


Gln Ala Glu Glu Arg Gly Val Val Ser Ile Lys Gly Val Cys Ala Asn 
65                  70                  75                  80  


Arg Tyr Leu Ala Met Lys Glu Asp Gly Arg Leu Tyr Ala Ile Lys Asn 
                85                  90                  95      


Val Thr Asp Glu Cys Phe Phe Phe Glu Arg Leu Glu Glu Asn Asn Tyr 
            100                 105                 110         


Asn Thr Tyr Arg Ser Arg Lys Tyr Pro Ser Trp Tyr Val Ala Leu Lys 
        115                 120                 125             


Arg Thr Gly Gln Tyr Lys Leu Gly Ser Lys Thr Gly Pro Gly Gln Lys 
    130                 135                 140                 


Ala Ile Leu Phe Leu Pro Met Ser Ala Lys Ser 
145                 150                 155 


<210>  6
<211>  435
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
ggatccatgt tcaacttacc ccccggcaac tacaagaagc cgaagctgct gtattgcagc       60

aatggcggcc actttctgcg cattttaccg gatggtaccg ttgatggtac ccgtgatcgt      120

tcagatccgc acatccagtt acagctgatc gcagaaagcg tgggtgaagt gtacatcaag      180

agcaccgaaa ccggccagta tctggcaatg gataccgatg gcctgctgta tggttcacaa      240

accccgaacg aagaatgcct gttcctggaa cgcctggaag aaaacggcta caacacctac      300

atcagcaaga agcacgcgga gaagaactgg tttgttggcc tgaacaagaa cggcagctgc      360

aaacgtggtc ctcgtaccca ttatggccag aaagcgattc tgtttctgcc gttaccggtt      420

agcagcgatg aattc                                                       435


<210>  7
<211>  480
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
ggatccatgg cagcaggtag cattactact ttaccggcgc tgccggaaga tggtggttca       60

ggtgcatttc ctcctggcca cttcaaagat cctaaacgcc tgtactgcaa gaatggcggc      120

ttctttctgc gcattcaccc ggatggccgt gttgatggtg ttcgcgaaaa atcagatccg      180

cacatcaagc tgcagttaca ggcggaagaa cgtggcgttg tgagcatcaa gggcgtttgt      240

gcaaaccgct atttagcgat gaaagaagac ggccgcctgt tagcgagcaa gtgtgtgacc      300

gacgaatgct tcttcttcga acgcctggaa agcaacaact acaacaccta ccgcagccgc      360

aagtacacca gctggtatgt tgcgttaaac cgtaccggcc agtacaaatt aggcagcaaa      420

accggcccgg gtcagaaagc gattctgttt ctgcctatga gcgcgaagag ctgagaattc      480


<210>  8
<211>  477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
ggatccatgg cagcaggttc gatcactaca ttaccggcac tgccggaaga tggtggttca       60

ggtgcatttc ctcctggcca cttcaaagac cctaaactgc tgtactgcaa gaatggcggc      120

ttctttctgc gcattcaccc ggatggccgt gttgatggta ctcgcgataa atcagatccg      180

ttcatcaagc tgcagctgca agcggaagaa cgtggcgtgg tgagcattaa gggcgtttgt      240

gcaaaccgtt atttagcgat gaaggaagac ggccgcctgt acgcgatcaa gaacgtgacc      300

gacgaatgct tcttctttga acgcctggaa gaaaacaact acaacaccta ccgcagccgc      360

aagtacccga gctggtatgt tgcgttaaag cgtaccggcc agtataaatt aggcagcaaa      420

accggtccgg gccagaaggc gattctgttt ctgcctatga gcgcgaagtc agaattc         477


<210>  9
<211>  213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
ggatccatga gccaccttgt gaaatgcgcc gagaaggaga agaccttttg cgtgaatggc       60

ggcgaatgct tcatggtgaa ggatctgtca aatccgagcc gctacctgtg caaatgcccg      120

aacgagttta ccggcgatcg ttgccagaat tacgttatgg cgagcttcta caagcacctg      180

ggcatcgagt tcatggaagc ggagtaagaa ttc                                   213


<210>  10
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10
ggatccgcgc tggataccaa ctattgcttt agcagcaccg aaaaaaactg ctgcgtgcgc       60

cagctgtata ttgattttcg caaagatctg ggctggaaat ggattcatga accgaaaggc      120

tatcatgcga acttttgcct gggcccgtgc ccgtatattt ggagcctgga tacccagtat      180

agcaaagtgc tggcgctgta taaccagcat aacccgggcg cgagcgcggc gccgtgctgc      240

gtgccgcagg cgctggaacc gctgccgatt gtgtattatg tgggccgcaa accgaaagtg      300

gaacagctga gcaacatgat tgtgcgcagc tgcaaatgca gctgagaatt c               351


<210>  11
<211>  277
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11
ggatccgcgc tggataccaa ctattgcttt agcagcaccg aaaaaaactg ctgcgtgcgc       60

cagctgtata ttgattttcg caaagatctg ggctggaaat ggattcatga accgaaaggc      120

tatcatgcga acttttgcct gggcccgtgc ccgtatattt ggagcctgga tacccagtat      180

agcaaagtgc tggcgctgta taaccagcat aacccgggcg cgagcgcggc gccgagctgc      240

gtgccgcagg cgctggaacc gctgccgatt gtgtatt                               277


<210>  12
<211>  273
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12
ggatccgcgc tggataccaa ctattgcttt cgcaacctgg aagaaaactg ctgcgtgcgc       60

ccgctgtata ttgattttcg ccaggatctg ggctggaaat gggtgcatga accgaaaggc      120

tattatgcga acttttgcag cggcccgtgc ccgtatctgc gcagcgcgga taccacccat      180

agcaccgtgc tgggcctgta taacaccctg aacccggaag cgagcgcgag cccgtgctgc      240

gtgccgcagg atctggaacc gctgaccatt ctg                                   273


<210>  13
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13
ggatccgcgc tggataccaa ctattgcttt cgcaacctgg aagaaaactg ctgcgtgcgc       60

ccgctgtata ttgattttcg ccaggatctg ggctggaaat gggtgcatga accgaaaggc      120

tattatgcga acttttgcag cggcccgtgc ccgtatctgc gcagcgcgga taccacccat      180

agcaccgtgc tgggcctgta taacaccctg aacccggaag cgagcgcgag cccgagctgc      240

gtgccgcagg atctggaacc gctgaccatt ctgtattatg tgggccgcac cccgaaagtg      300

gaacagctga gcaacatggt ggtgaaaagc tgcaaatgca gctgaaggga attc            354


<210>  14
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14

Ala Ala Gly Ser Ile Thr Thr Leu Pro Ala Leu Pro Glu Asp Gly Gly 
1               5                   10                  15      


Ser Gly Ala Phe Pro Pro Gly His Phe Lys Asp Pro Lys Arg Leu Tyr 
            20                  25                  30          


Cys Lys Asn Gly Gly Phe Phe Leu Arg Ile His Pro Asp Gly Arg Val 
        35                  40                  45              


Asp Gly Val Arg Glu Lys Ser Asp Pro His Ile Lys Leu Gln Leu Gln 
    50                  55                  60                  


Ala Glu Glu Arg Gly Val Val Ser Ile Lys Gly Val Cys Ala Asn Arg 
65                  70                  75                  80  


Tyr Leu Ala Met Lys Glu Asp Gly Arg Leu Leu Ala Ser Lys Cys Val 
                85                  90                  95      


Thr Asp Glu Cys Phe Phe Phe Glu Arg Leu Glu Ser Asn Asn Tyr Asn 
            100                 105                 110         


Thr Tyr Arg Ser Arg Lys Tyr Thr Ser Trp Tyr Val Ala Leu Lys Arg 
        115                 120                 125             


Thr Gly Gln Tyr Lys Leu Gly Ser Lys Thr Gly Pro Gly Gln Lys Ala 
    130                 135                 140                 


Ile Leu Phe Leu Pro Met Ser Ala Lys Ser 
145                 150                 


<210>  15
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15

Ala Ala Gly Ser Ile Thr Thr Leu Pro Ala Leu Pro Glu Asp Gly Gly 
1               5                   10                  15      


Ser Gly Ala Phe Pro Pro Gly His Phe Lys Asp Pro Lys Leu Leu Tyr 
            20                  25                  30          


Cys Lys Asn Gly Gly Phe Phe Leu Arg Ile His Pro Asp Gly Arg Val 
        35                  40                  45              


Asp Gly Thr Arg Asp Lys Ser Asp Pro Phe Ile Lys Leu Gln Leu Gln 
    50                  55                  60                  


Ala Glu Glu Arg Gly Val Val Ser Ile Lys Gly Val Cys Ala Asn Arg 
65                  70                  75                  80  


Tyr Leu Ala Met Lys Glu Asp Gly Arg Leu Tyr Ala Ile Lys Asn Val 
                85                  90                  95      


Thr Asp Glu Cys Phe Phe Phe Glu Arg Leu Glu Glu Asn Asn Tyr Asn 
            100                 105                 110         


Thr Tyr Arg Ser Arg Lys Tyr Pro Ser Trp Tyr Val Ala Leu Lys Arg 
        115                 120                 125             


Thr Gly Gln Tyr Lys Leu Gly Ser Lys Thr Gly Pro Gly Gln Lys Ala 
    130                 135                 140                 


Ile Leu Phe Leu Pro Met Ser Ala Lys Ser 
145                 150                 


<210>  16
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16

Ala Leu Asp Thr Asn Tyr Cys Phe Arg Asn Leu Glu Glu Asn Cys Cys 
1               5                   10                  15      


Val Arg Pro Leu Tyr Ile Asp Phe Arg Gln Asp Leu Gly Trp Lys Trp 
            20                  25                  30          


Val His Glu Pro Lys Gly Tyr Tyr Ala Asn Phe Cys Ser Gly Pro Cys 
        35                  40                  45              


Pro Tyr Leu Arg Ser Ala Asp Thr Thr His Ser Thr Val Leu Gly Leu 
    50                  55                  60                  


Tyr Asn Thr Leu Asn Pro Glu Ala Ser Ala Ser Pro Cys Cys Val Pro 
65                  70                  75                  80  


Gln Asp Leu Glu Pro Leu Thr Ile Leu Tyr Tyr Val Gly Arg Thr Pro 
                85                  90                  95      


Lys Val Glu Gln Leu Ser Asn Met Val Val Lys Ser Cys Lys Cys Ser 
            100                 105                 110         


<210>  17
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17

Ser His Leu Val Lys Cys Ala Glu Lys Glu Lys Thr Phe Cys Val Asn 
1               5                   10                  15      


Gly Gly Glu Cys Phe Met Val Lys Asp Leu Ser Asn Pro Ser Arg Tyr 
            20                  25                  30          


Leu Cys Lys Cys Pro Asn Glu Phe Thr Gly Asp Arg Cys Gln Asn Tyr 
        35                  40                  45              


Val Met Ala Ser Phe Tyr Lys His Leu Gly Ile Glu Phe Met Glu Ala 
    50                  55                  60                  


Glu 
65  


