               SEQUENCE LISTING

<110> GENETHON
      UNIVERSITE D'EVRY-VAL-D'ESSONNE
      INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE
      MEDICALE)

<120> GENE THERAPY EXPRESSION SYSTEM ALLEVIATING CARDIAC TOXICITY OF FKRP

<130> G143-B-57711 PCT

<150> EP19306134.8
<151> 2019-09-19

<160> 21

<170> BiSSAP 1.3.6

<210> 1
<211> 4057
<212> DNA
<213> Artificial Sequence


<220> 
<223> K7 AAV-FKRP

<400> 1
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc       60

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg      120

gccaactcca tcactagggg ttccttaccc cctgcccccc acagctcctc tcctgtgcct      180

tgtttcccag ccatgcgttc tcctctataa atacccgctc tggtatttgg ggttggcagc      240

tgttgctgcc agggagatgg ttgggttgac atgcggctcc tgacaaaaca caaacccctg      300

gtgtgtgtgg gcgtgggtgg tgtgagtagg gggatgaatc agggaggggg cgggggaccc      360

agggggcagg agccacacaa agtctgtgcg ggggtgggag cgcacatagc aattggaaac      420

tgaaagctta tcagaccctt tctggaaatc agcccactgt ttataaactt gaggccccac      480

cctcgacagt accggggagg aagagggcct gcactagtcc agagggaaac tgaggctcag      540

ggctagctcg cccatagaca tacatggcag gcaggctttg gccaggatcc ctccgcctgc      600

caggcgtctc cctgccctcc cttcctgcct agagaccccc accctcaagc ctggctggtc      660

tttgcctgag acccaaacct cttcgacttc aagagaatat ttaggaacaa ggtggtttag      720

ggcctttcct gggaacaggc cttgaccctt taagaaatga cccaaagtct ctccttgacc      780

aaaaagggga ccctcaaact aaagggaagc ctctcttctg ctgtctcccc tgaccccact      840

cccccccacc ccaggacgag gagataacca gggctgaaag aggcccgcct gggggctgca      900

gacatgcttg ctgcctgccc tggcgaagga ttggcaggct tgcccgtcac aggacccccg      960

ctggctgact caggggcgca ggcctcttgc gggggagctg gcctccccgc ccccacggcc     1020

acgggccgcc ctttcctggc aggacagcgg gatcttgcag ctgtcagggg aggggaggcg     1080

ggggctgatg tcaggaggga tacaaatagt gccgacggct gggggccctg tctcccctcg     1140

ccgcatccac tctccggccg gccgcctgcc cgccgcctcc tccgtgcgcc cgccagcctc     1200

gcccgcgtac acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc     1260

tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc taatctcttt     1320

ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta aagaataaca     1380

gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt tctgcatata     1440

aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt     1500

ctgcttttat tttttggttg ggataaggct ggattattct gagtccaagc taggcccttt     1560

tgctaatctt gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct     1620

ctgtgctggc ccatcacttt ggcaaagaat tcgccaccat gagactgacc aggtgccagg     1680

ctgccctggc tgctgccatc accctgaacc tgctggtgct gttctatgtg agctggctgc     1740

agcaccagcc caggaacagc agggccaggg gccccaggag ggcctctgct gctggcccca     1800

gggtgacagt gctggtgagg gagtttgagg cctttgacaa tgctgtgcct gagctggtgg     1860

acagcttcct gcagcaggac cctgcccagc ctgtggtggt ggctgctgat accctgccct     1920

acccccccct ggccctgccc aggatcccca atgtgaggct ggccctgctg cagcctgccc     1980

tggacaggcc tgctgctgcc agcaggcctg agacctatgt ggccacagag tttgtggccc     2040

tggtgcctga tggggccagg gctgaggccc ctggcctgct ggagaggatg gtggaggccc     2100

tgagggctgg ctctgccagg ctggtggctg cccctgtggc cacagccaac cctgccaggt     2160

gcctggccct gaatgtgagc ctgagagagt ggacagccag gtatggggct gcccctgctg     2220

cccccaggtg tgatgccctg gatggagatg ctgtggtgct gctgagggcc agggacctgt     2280

tcaacctgtc tgcccccctg gccaggcctg tggggaccag cctgtttctg cagacagccc     2340

tgaggggctg ggctgtgcag ctgctggacc tgacctttgc tgctgccagg cagccccccc     2400

tggctacagc ccacgccagg tggaaggctg agagggaggg cagggccagg agggctgccc     2460

tgctgagggc cctggggatc aggctggtga gctgggaggg gggcaggctg gagtggtttg     2520

gctgcaacaa ggagacaacc aggtgctttg ggacagtggt gggggatacc cctgcctacc     2580

tgtatgagga gaggtggacc cccccctgct gcctgagggc cctgagggag acagccaggt     2640

atgtggtggg ggtgctggag gctgctgggg tgaggtactg gctggagggg ggcagcctgc     2700

tgggggctgc caggcacggg gacattatcc cctgggacta tgatgtggac ctgggcatct     2760

acctggagga tgtgggcaac tgtgagcagc tgaggggggc tgaggctggc tctgtggtgg     2820

atgagagggg ctttgtgtgg gagaaggctg tggaggggga ctttttcagg gtgcagtact     2880

ctgagagcaa ccacctgcac gtggacctgt ggcccttcta ccccaggaat ggggtgatga     2940

ccaaggacac ctggctggac cacaggcagg atgtggagtt ccctgagcac ttcctgcagc     3000

ccctggtgcc cctgcccttt gctggctttg tggcccaggc ccccaacaac tacaggaggt     3060

tcctggagct gaagtttggc cctggggtga ttgagaaccc ccagtacccc aaccctgccc     3120

tgctgagcct gacaggctct ggctgaattc accccaccag tgcaggctgc ctatcagaaa     3180

gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg ctttcttgct     3240

gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac tgggggatat     3300

tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt tcattgcaat     3360

gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag gtcagtgcat     3420

ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact atatcttaaa     3480

ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag ccctgatgcc     3540

tatgccttat tcatccctca gaaaaggatt caagtagagg cttgatttgg aggttaaagt     3600

tttgctatgc tgtattttac attacttatt gttttagctg tcctcatgaa tgtcttttca     3660

ctacccattt gcttatcctg catctctcag ccttgactcc actcagttct cttgcttaga     3720

gataccacct ttcccctgaa gtgttccttc catgttttac ggcgagatgg tttctcctcg     3780

cctggccact cagccttagt tgtctctgtt gtcttataga ggtctacttg aagaaggaaa     3840

aacagggggc atggtttgac tgtcctgtga gcccttcttc cctgcctccc ccactcacag     3900

tgacccggaa tcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct     3960

cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct     4020

cagtgagcga gcgagcgcgc agagagggag tggccaa                              4057


<210> 2
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> miR208a target sequence

<400> 2
acaagctttt tgctcgtctt at                                             22


<210> 3
<211> 4091
<212> DNA
<213> Artificial Sequence


<220> 
<223> K7 AAV-FKRP-single

<400> 3
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc       60

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg      120

gccaactcca tcactagggg ttccttaccc cctgcccccc acagctcctc tcctgtgcct      180

tgtttcccag ccatgcgttc tcctctataa atacccgctc tggtatttgg ggttggcagc      240

tgttgctgcc agggagatgg ttgggttgac atgcggctcc tgacaaaaca caaacccctg      300

gtgtgtgtgg gcgtgggtgg tgtgagtagg gggatgaatc agggaggggg cgggggaccc      360

agggggcagg agccacacaa agtctgtgcg ggggtgggag cgcacatagc aattggaaac      420

tgaaagctta tcagaccctt tctggaaatc agcccactgt ttataaactt gaggccccac      480

cctcgacagt accggggagg aagagggcct gcactagtcc agagggaaac tgaggctcag      540

ggctagctcg cccatagaca tacatggcag gcaggctttg gccaggatcc ctccgcctgc      600

caggcgtctc cctgccctcc cttcctgcct agagaccccc accctcaagc ctggctggtc      660

tttgcctgag acccaaacct cttcgacttc aagagaatat ttaggaacaa ggtggtttag      720

ggcctttcct gggaacaggc cttgaccctt taagaaatga cccaaagtct ctccttgacc      780

aaaaagggga ccctcaaact aaagggaagc ctctcttctg ctgtctcccc tgaccccact      840

cccccccacc ccaggacgag gagataacca gggctgaaag aggcccgcct gggggctgca      900

gacatgcttg ctgcctgccc tggcgaagga ttggcaggct tgcccgtcac aggacccccg      960

ctggctgact caggggcgca ggcctcttgc gggggagctg gcctccccgc ccccacggcc     1020

acgggccgcc ctttcctggc aggacagcgg gatcttgcag ctgtcagggg aggggaggcg     1080

ggggctgatg tcaggaggga tacaaatagt gccgacggct gggggccctg tctcccctcg     1140

ccgcatccac tctccggccg gccgcctgcc cgccgcctcc tccgtgcgcc cgccagcctc     1200

gcccgcgtac acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc     1260

tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc taatctcttt     1320

ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta aagaataaca     1380

gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt tctgcatata     1440

aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt     1500

ctgcttttat tttttggttg ggataaggct ggattattct gagtccaagc taggcccttt     1560

tgctaatctt gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct     1620

ctgtgctggc ccatcacttt ggcaaagaat tcgccaccat gagactgacc aggtgccagg     1680

ctgccctggc tgctgccatc accctgaacc tgctggtgct gttctatgtg agctggctgc     1740

agcaccagcc caggaacagc agggccaggg gccccaggag ggcctctgct gctggcccca     1800

gggtgacagt gctggtgagg gagtttgagg cctttgacaa tgctgtgcct gagctggtgg     1860

acagcttcct gcagcaggac cctgcccagc ctgtggtggt ggctgctgat accctgccct     1920

acccccccct ggccctgccc aggatcccca atgtgaggct ggccctgctg cagcctgccc     1980

tggacaggcc tgctgctgcc agcaggcctg agacctatgt ggccacagag tttgtggccc     2040

tggtgcctga tggggccagg gctgaggccc ctggcctgct ggagaggatg gtggaggccc     2100

tgagggctgg ctctgccagg ctggtggctg cccctgtggc cacagccaac cctgccaggt     2160

gcctggccct gaatgtgagc ctgagagagt ggacagccag gtatggggct gcccctgctg     2220

cccccaggtg tgatgccctg gatggagatg ctgtggtgct gctgagggcc agggacctgt     2280

tcaacctgtc tgcccccctg gccaggcctg tggggaccag cctgtttctg cagacagccc     2340

tgaggggctg ggctgtgcag ctgctggacc tgacctttgc tgctgccagg cagccccccc     2400

tggctacagc ccacgccagg tggaaggctg agagggaggg cagggccagg agggctgccc     2460

tgctgagggc cctggggatc aggctggtga gctgggaggg gggcaggctg gagtggtttg     2520

gctgcaacaa ggagacaacc aggtgctttg ggacagtggt gggggatacc cctgcctacc     2580

tgtatgagga gaggtggacc cccccctgct gcctgagggc cctgagggag acagccaggt     2640

atgtggtggg ggtgctggag gctgctgggg tgaggtactg gctggagggg ggcagcctgc     2700

tgggggctgc caggcacggg gacattatcc cctgggacta tgatgtggac ctgggcatct     2760

acctggagga tgtgggcaac tgtgagcagc tgaggggggc tgaggctggc tctgtggtgg     2820

atgagagggg ctttgtgtgg gagaaggctg tggaggggga ctttttcagg gtgcagtact     2880

ctgagagcaa ccacctgcac gtggacctgt ggcccttcta ccccaggaat ggggtgatga     2940

ccaaggacac ctggctggac cacaggcagg atgtggagtt ccctgagcac ttcctgcagc     3000

ccctggtgcc cctgcccttt gctggctttg tggcccaggc ccccaacaac tacaggaggt     3060

tcctggagct gaagtttggc cctggggtga ttgagaaccc ccagtacccc aaccctgccc     3120

tgctgagcct gacaggctct ggctgatcta gaacaagctt tttgctcgtc ttatctcgag     3180

attcacccca ccagtgcagg ctgcctatca gaaagtggtg gctggtgtgg ctaatgccct     3240

ggcccacaag tatcactaag ctcgctttct tgctgtccaa tttctattaa aggttccttt     3300

gttccctaag tccaactact aaactggggg atattatgaa gggccttgag catctggatt     3360

ctgcctaata aaaaacattt attttcattg caatgatgta tttaaattat ttctgaatat     3420

tttactaaaa agggaatgtg ggaggtcagt gcatttaaaa cataaagaaa tgaagagcta     3480

gttcaaacct tgggaaaata cactatatct taaactccat gaaagaaggt gaggctgcaa     3540

acagctaatg cacattggca acagccctga tgcctatgcc ttattcatcc ctcagaaaag     3600

gattcaagta gaggcttgat ttggaggtta aagttttgct atgctgtatt ttacattact     3660

tattgtttta gctgtcctca tgaatgtctt ttcactaccc atttgcttat cctgcatctc     3720

tcagccttga ctccactcag ttctcttgct tagagatacc acctttcccc tgaagtgttc     3780

cttccatgtt ttacggcgag atggtttctc ctcgcctggc cactcagcct tagttgtctc     3840

tgttgtctta tagaggtcta cttgaagaag gaaaaacagg gggcatggtt tgactgtcct     3900

gtgagccctt cttccctgcc tcccccactc acagtgaccc ggaatcagga acccctagtg     3960

atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag     4020

gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagagag     4080

ggagtggcca a                                                          4091


<210> 4
<211> 4119
<212> DNA
<213> Artificial Sequence


<220> 
<223> K7 AAV-FKRP-tandem

<400> 4
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc       60

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg      120

gccaactcca tcactagggg ttccttaccc cctgcccccc acagctcctc tcctgtgcct      180

tgtttcccag ccatgcgttc tcctctataa atacccgctc tggtatttgg ggttggcagc      240

tgttgctgcc agggagatgg ttgggttgac atgcggctcc tgacaaaaca caaacccctg      300

gtgtgtgtgg gcgtgggtgg tgtgagtagg gggatgaatc agggaggggg cgggggaccc      360

agggggcagg agccacacaa agtctgtgcg ggggtgggag cgcacatagc aattggaaac      420

tgaaagctta tcagaccctt tctggaaatc agcccactgt ttataaactt gaggccccac      480

cctcgacagt accggggagg aagagggcct gcactagtcc agagggaaac tgaggctcag      540

ggctagctcg cccatagaca tacatggcag gcaggctttg gccaggatcc ctccgcctgc      600

caggcgtctc cctgccctcc cttcctgcct agagaccccc accctcaagc ctggctggtc      660

tttgcctgag acccaaacct cttcgacttc aagagaatat ttaggaacaa ggtggtttag      720

ggcctttcct gggaacaggc cttgaccctt taagaaatga cccaaagtct ctccttgacc      780

aaaaagggga ccctcaaact aaagggaagc ctctcttctg ctgtctcccc tgaccccact      840

cccccccacc ccaggacgag gagataacca gggctgaaag aggcccgcct gggggctgca      900

gacatgcttg ctgcctgccc tggcgaagga ttggcaggct tgcccgtcac aggacccccg      960

ctggctgact caggggcgca ggcctcttgc gggggagctg gcctccccgc ccccacggcc     1020

acgggccgcc ctttcctggc aggacagcgg gatcttgcag ctgtcagggg aggggaggcg     1080

ggggctgatg tcaggaggga tacaaatagt gccgacggct gggggccctg tctcccctcg     1140

ccgcatccac tctccggccg gccgcctgcc cgccgcctcc tccgtgcgcc cgccagcctc     1200

gcccgcgtac acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc     1260

tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc taatctcttt     1320

ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta aagaataaca     1380

gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt tctgcatata     1440

aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt     1500

ctgcttttat tttttggttg ggataaggct ggattattct gagtccaagc taggcccttt     1560

tgctaatctt gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct     1620

ctgtgctggc ccatcacttt ggcaaagaat tcgccaccat gagactgacc aggtgccagg     1680

ctgccctggc tgctgccatc accctgaacc tgctggtgct gttctatgtg agctggctgc     1740

agcaccagcc caggaacagc agggccaggg gccccaggag ggcctctgct gctggcccca     1800

gggtgacagt gctggtgagg gagtttgagg cctttgacaa tgctgtgcct gagctggtgg     1860

acagcttcct gcagcaggac cctgcccagc ctgtggtggt ggctgctgat accctgccct     1920

acccccccct ggccctgccc aggatcccca atgtgaggct ggccctgctg cagcctgccc     1980

tggacaggcc tgctgctgcc agcaggcctg agacctatgt ggccacagag tttgtggccc     2040

tggtgcctga tggggccagg gctgaggccc ctggcctgct ggagaggatg gtggaggccc     2100

tgagggctgg ctctgccagg ctggtggctg cccctgtggc cacagccaac cctgccaggt     2160

gcctggccct gaatgtgagc ctgagagagt ggacagccag gtatggggct gcccctgctg     2220

cccccaggtg tgatgccctg gatggagatg ctgtggtgct gctgagggcc agggacctgt     2280

tcaacctgtc tgcccccctg gccaggcctg tggggaccag cctgtttctg cagacagccc     2340

tgaggggctg ggctgtgcag ctgctggacc tgacctttgc tgctgccagg cagccccccc     2400

tggctacagc ccacgccagg tggaaggctg agagggaggg cagggccagg agggctgccc     2460

tgctgagggc cctggggatc aggctggtga gctgggaggg gggcaggctg gagtggtttg     2520

gctgcaacaa ggagacaacc aggtgctttg ggacagtggt gggggatacc cctgcctacc     2580

tgtatgagga gaggtggacc cccccctgct gcctgagggc cctgagggag acagccaggt     2640

atgtggtggg ggtgctggag gctgctgggg tgaggtactg gctggagggg ggcagcctgc     2700

tgggggctgc caggcacggg gacattatcc cctgggacta tgatgtggac ctgggcatct     2760

acctggagga tgtgggcaac tgtgagcagc tgaggggggc tgaggctggc tctgtggtgg     2820

atgagagggg ctttgtgtgg gagaaggctg tggaggggga ctttttcagg gtgcagtact     2880

ctgagagcaa ccacctgcac gtggacctgt ggcccttcta ccccaggaat ggggtgatga     2940

ccaaggacac ctggctggac cacaggcagg atgtggagtt ccctgagcac ttcctgcagc     3000

ccctggtgcc cctgcccttt gctggctttg tggcccaggc ccccaacaac tacaggaggt     3060

tcctggagct gaagtttggc cctggggtga ttgagaaccc ccagtacccc aaccctgccc     3120

tgctgagcct gacaggctct ggctgatcta gaacaagctt tttgctcgtc ttatcctagg     3180

acaagctttt tgctcgtctt atctcgagat tcaccccacc agtgcaggct gcctatcaga     3240

aagtggtggc tggtgtggct aatgccctgg cccacaagta tcactaagct cgctttcttg     3300

ctgtccaatt tctattaaag gttcctttgt tccctaagtc caactactaa actgggggat     3360

attatgaagg gccttgagca tctggattct gcctaataaa aaacatttat tttcattgca     3420

atgatgtatt taaattattt ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc     3480

atttaaaaca taaagaaatg aagagctagt tcaaaccttg ggaaaataca ctatatctta     3540

aactccatga aagaaggtga ggctgcaaac agctaatgca cattggcaac agccctgatg     3600

cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa     3660

gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg aatgtctttt     3720

cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt ctcttgctta     3780

gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat ggtttctcct     3840

cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact tgaagaagga     3900

aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc ccccactcac     3960

agtgacccgg aatcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg     4020

ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc     4080

ctcagtgagc gagcgagcgc gcagagaggg agtggccaa                            4119


<210> 5
<211> 495
<212> PRT
<213> Homo sapiens


<220> 
<223> FKRP

<400> 5
Met Arg Leu Thr Arg Cys Gln Ala Ala Leu Ala Ala Ala Ile Thr Leu 
1               5                   10                  15      
Asn Leu Leu Val Leu Phe Tyr Val Ser Trp Leu Gln His Gln Pro Arg 
            20                  25                  30          
Asn Ser Arg Ala Arg Gly Pro Arg Arg Ala Ser Ala Ala Gly Pro Arg 
        35                  40                  45              
Val Thr Val Leu Val Arg Glu Phe Glu Ala Phe Asp Asn Ala Val Pro 
    50                  55                  60                  
Glu Leu Val Asp Ser Phe Leu Gln Gln Asp Pro Ala Gln Pro Val Val 
65                  70                  75                  80  
Val Ala Ala Asp Thr Leu Pro Tyr Pro Pro Leu Ala Leu Pro Arg Ile 
                85                  90                  95      
Pro Asn Val Arg Leu Ala Leu Leu Gln Pro Ala Leu Asp Arg Pro Ala 
            100                 105                 110         
Ala Ala Ser Arg Pro Glu Thr Tyr Val Ala Thr Glu Phe Val Ala Leu 
        115                 120                 125             
Val Pro Asp Gly Ala Arg Ala Glu Ala Pro Gly Leu Leu Glu Arg Met 
    130                 135                 140                 
Val Glu Ala Leu Arg Ala Gly Ser Ala Arg Leu Val Ala Ala Pro Val 
145                 150                 155                 160 
Ala Thr Ala Asn Pro Ala Arg Cys Leu Ala Leu Asn Val Ser Leu Arg 
                165                 170                 175     
Glu Trp Thr Ala Arg Tyr Gly Ala Ala Pro Ala Ala Pro Arg Cys Asp 
            180                 185                 190         
Ala Leu Asp Gly Asp Ala Val Val Leu Leu Arg Ala Arg Asp Leu Phe 
        195                 200                 205             
Asn Leu Ser Ala Pro Leu Ala Arg Pro Val Gly Thr Ser Leu Phe Leu 
    210                 215                 220                 
Gln Thr Ala Leu Arg Gly Trp Ala Val Gln Leu Leu Asp Leu Thr Phe 
225                 230                 235                 240 
Ala Ala Ala Arg Gln Pro Pro Leu Ala Thr Ala His Ala Arg Trp Lys 
                245                 250                 255     
Ala Glu Arg Glu Gly Arg Ala Arg Arg Ala Ala Leu Leu Arg Ala Leu 
            260                 265                 270         
Gly Ile Arg Leu Val Ser Trp Glu Gly Gly Arg Leu Glu Trp Phe Gly 
        275                 280                 285             
Cys Asn Lys Glu Thr Thr Arg Cys Phe Gly Thr Val Val Gly Asp Thr 
    290                 295                 300                 
Pro Ala Tyr Leu Tyr Glu Glu Arg Trp Thr Pro Pro Cys Cys Leu Arg 
305                 310                 315                 320 
Ala Leu Arg Glu Thr Ala Arg Tyr Val Val Gly Val Leu Glu Ala Ala 
                325                 330                 335     
Gly Val Arg Tyr Trp Leu Glu Gly Gly Ser Leu Leu Gly Ala Ala Arg 
            340                 345                 350         
His Gly Asp Ile Ile Pro Trp Asp Tyr Asp Val Asp Leu Gly Ile Tyr 
        355                 360                 365             
Leu Glu Asp Val Gly Asn Cys Glu Gln Leu Arg Gly Ala Glu Ala Gly 
    370                 375                 380                 
Ser Val Val Asp Glu Arg Gly Phe Val Trp Glu Lys Ala Val Glu Gly 
385                 390                 395                 400 
Asp Phe Phe Arg Val Gln Tyr Ser Glu Ser Asn His Leu His Val Asp 
                405                 410                 415     
Leu Trp Pro Phe Tyr Pro Arg Asn Gly Val Met Thr Lys Asp Thr Trp 
            420                 425                 430         
Leu Asp His Arg Gln Asp Val Glu Phe Pro Glu His Phe Leu Gln Pro 
        435                 440                 445             
Leu Val Pro Leu Pro Phe Ala Gly Phe Val Ala Gln Ala Pro Asn Asn 
    450                 455                 460                 
Tyr Arg Arg Phe Leu Glu Leu Lys Phe Gly Pro Gly Val Ile Glu Asn 
465                 470                 475                 480 
Pro Gln Tyr Pro Asn Pro Ala Leu Leu Ser Leu Thr Gly Ser Gly 
                485                 490                 495 

<210> 6
<211> 1061
<212> DNA
<213> Artificial Sequence


<220> 
<223> Desmin promoter

<400> 6
taccccctgc cccccacagc tcctctcctg tgccttgttt cccagccatg cgttctcctc      60

tataaatacc cgctctggta tttggggttg gcagctgttg ctgccaggga gatggttggg     120

ttgacatgcg gctcctgaca aaacacaaac ccctggtgtg tgtgggcgtg ggtggtgtga     180

gtagggggat gaatcaggga gggggcgggg gacccagggg gcaggagcca cacaaagtct     240

gtgcgggggt gggagcgcac atagcaattg gaaactgaaa gcttatcaga ccctttctgg     300

aaatcagccc actgtttata aacttgaggc cccaccctcg acagtaccgg ggaggaagag     360

ggcctgcact agtccagagg gaaactgagg ctcagggcta gctcgcccat agacatacat     420

ggcaggcagg ctttggccag gatccctccg cctgccaggc gtctccctgc cctcccttcc     480

tgcctagaga cccccaccct caagcctggc tggtctttgc ctgagaccca aacctcttcg     540

acttcaagag aatatttagg aacaaggtgg tttagggcct ttcctgggaa caggccttga     600

ccctttaaga aatgacccaa agtctctcct tgaccaaaaa ggggaccctc aaactaaagg     660

gaagcctctc ttctgctgtc tcccctgacc ccactccccc ccaccccagg acgaggagat     720

aaccagggct gaaagaggcc cgcctggggg ctgcagacat gcttgctgcc tgccctggcg     780

aaggattggc aggcttgccc gtcacaggac ccccgctggc tgactcaggg gcgcaggcct     840

cttgcggggg agctggcctc cccgccccca cggccacggg ccgccctttc ctggcaggac     900

agcgggatct tgcagctgtc aggggagggg aggcgggggc tgatgtcagg agggatacaa     960

atagtgccga cggctggggg ccctgtctcc cctcgccgca tccactctcc ggccggccgc    1020

ctgcccgccg cctcctccgt gcgcccgcca gcctcgcccg c                        1061


<210> 7
<211> 1654
<212> DNA
<213> Artificial Sequence


<220> 
<223> Calpain 3 promoter

<400> 7
cacatgcctc cactctgcca tacttgaaat gtgctcatct ccttacagcc cagggagcag      60

ctattgtggg tagaagacaa ggtggaggcc aggcaggcac ttcccttccc cagagccact     120

tatgctctca tctaagagcc ctgaaaccag gtgtgacatc ccaggagttg acagacagtc     180

tggttcagta tctaattcca acttctgtct cagatgccta atgtggcatg gctgaatgag     240

tcaacatata acctgtacag taagtcctca cttaacatca ttgataggtg cttgtaaact     300

gtgactttaa cgaaaacata ccgtgtgctg tagggactta actcttgttt atatcagtta     360

gcctggtttc actatacagt acatcatttt gcttaaagtc acagcttacg agaacctatc     420

gatgatgtta agtgaggatt ttctctgctc aggtgcactt tttttttttt tttaagacgg     480

agtctctttc tgtcacctgg gctggagtgc agtggcgcga tctgggttca ctacaacctc     540

tgcctcctgg gttcaagcaa ttcttctgtc tcagcctccc aagtagctgg gattacaggc     600

acccgccgcc acacccggct tatttttgta tttttagtag agacagggtt tcactattgt     660

tggccatgct ggtctcgaac tcctgacctc atgtgatcca cccgcctcgg cctcccaaag     720

tgcagagatt agagacgtga gccacatggc ccagcaggac cactttttag cagattcagt     780

cccagtgttc attttgtgga tggggagaga caagaggtgg caaggtcaag tgtgcaggta     840

gagacaggga ttttctcaaa tgaggactct gctgagtagc attttccatg cagacatttc     900

caatgagcgc tgacccaaga acattctaaa aaagatacca aatctaacat tgaataatgt     960

tctgatatcc taaaatttta ggactaaaaa tcatgttctc taaaattcac agaatatttt    1020

tgtagaattc agtacctccc gttcacccta actagctttt ttgcaatatt gttttccatt    1080

catttgatgg ccagtagttg ggtggtctgt ataactgcct actcaataac atgtcagcag    1140

ttctcagctt ctttccagtg ttcaccttac tcagatactc ccttttcatt ttctggcaac    1200

accagcactt catggcaaca gaaatgtccc tagccaggtt ctctctctac catgcagtct    1260

ctcttgctct catactcaca gtgtttcttc acatctattt ttagttttcc tggctcaagc    1320

atcttcaggc cactgaaaca caaccctcac tctctttctc tctccctctg gcatgcatgc    1380

tgctggtagg agacccccaa gtcaacattg cttcagaaat cctttagcac tcatttctca    1440

ggagaactta tggcttcaga atcacagctc ggtttttaag atggacataa cctgtacgac    1500

cttctgatgg gctttcaact ttgaactgga tgtggacact tttctctcag atgacagaat    1560

tactccaact tcccctttgc agttgcttcc tttccttgaa ggtagctgta tcttattttc    1620

tttaaaaagc tttttcttcc aaagccactt gcca                                1654


<210> 8
<211> 805
<212> DNA
<213> Artificial Sequence


<220> 
<223> miR206 promoter

<400> 8
gggggccaac tcttcctttg gcatatgttt ccccattttc tggcagagaa tcagatacca      60

caaagttcaa aaccccatct ccctccagcc agggtggcca tccagaccct gagtggctca     120

acagctgcca atgtccctca tccttctgag gctcaggcct cacagattgt ggggcaggtg     180

atgggctagg gggagcagaa gcccgacaaa aggatccttc ccacagtgaa caatggtgct     240

tggaatgctg gatgggcagc tgctgcccat caacaagcac ccaaaacaga tagacgtaca     300

gtaggaagta caggagggcc ggtgtgtttc taagcatgag tggctctctg cgtgaatgtg     360

gaaaatttct ctgttggatt ctctcttctt tttaattttc ccttcactgg atcccaaaca     420

ttaaaaaaga atcacattca aaatgcacaa aaacagcagc agtgaattaa ttagtagtaa     480

taacaaagga ctggatagac tgtagctgca caagaataag ccagggaaac gtggtgctgc     540

ttatctgtga acaaacagta ggaaggattt ggtcccaagc agcactgcca ttcctcacaa     600

cagatttatt tcagcatgat ttggtcgggc gggggggatt taggatgagt tgagatccca     660

gtgatcttct cgctaagagt ttcctgcctg ggcaaggagg aaagatgcta caagtggccc     720

acttctgaga tgcgggctgc ttctggatga cactgcttcc cgaggccaca tgcttcttta     780

tatccccata tggattactt tgcta                                           805


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> FKRPopt Forward

<400> 9
gcccttctac cccaggaatg                                                20


<210> 10
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> FKRPopt Reverse

<400> 10
aaacttcagc tccaggaacc tc                                             22


<210> 11
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> FKRPopt Probe

<400> 11
tgccctttgc tggctttgtg gcccaggc                                       28


<210> 12
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> HBB2pA Forward

<400> 12
cttgactcca ctcagttctc ttgct                                          25


<210> 13
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> HBB2pA Reverse

<400> 13
ccaggcgagg agaaacca                                                  18


<210> 14
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> HBB2pA Probe

<400> 14
ctcgccgtaa aacatggaag gaacacttc                                      29


<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> TTN Forward

<400> 15
gtcccctgcg tatctgctat g                                              21


<210> 16
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> TTN Reverse

<400> 16
cgctcgtttt caatactacc tctct                                          25


<210> 17
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> TTN Probe

<400> 17
tccgcagctc tagtggaaga accacc                                         26


<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> P0 Forward

<400> 18
ctccaagcag atgcagcaga                                                20


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> P0 Reverse

<400> 19
atagccttgc gcatcatggt                                                20


<210> 20
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> P0 Probe

<400> 20
ccgtggtgct gatgggcaag aa                                             22


<210> 21
<211> 22
<212> RNA
<213> Artificial Sequence


<220> 
<223> miR208a

<400> 21
auaagacgag caaaaagcuu gu                                              22
