                         SEQUENCE LISTING

<110>  MODERNATX, INC.
 
<120>  LNP-FORMULATED MRNA THERAPEUTICS AND USE THEREOF FOR TREATING 
       HUMAN SUBJECTS

<130>  MDN-830PC

<140>  PCT/US2020/050546
<141>  2020-09-11

<150>  US 62/899,095
<151>  2019-09-11

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: CHIKV-24 heavy chain ORF Sequence (Amino Acid)

<400>  1

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val 
            20                  25                  30          


Gln Pro Gly Lys Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr 
        35                  40                  45              


Phe Arg Asn Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Asp Trp Val Ala Leu Ile Ser Tyr Asp Gly Thr His Lys Tyr Tyr 
65                  70                  75                  80  


Lys Asp Ser Leu Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Phe Gln 
                85                  90                  95      


Asn Thr Val Asp Leu Gln Ile Asn Ser Leu Arg Pro Asp Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Lys Glu Leu Ala Thr Ser Gly Val Val Glu Pro 
        115                 120                 125             


Leu Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr 
145                 150                 155                 160 


Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                165                 170                 175     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            180                 185                 190         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        195                 200                 205             


Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile 
    210                 215                 220                 


Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val 
225                 230                 235                 240 


Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
                245                 250                 255     


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            260                 265                 270         


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        275                 280                 285             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    290                 295                 300                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
305                 310                 315                 320 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                325                 330                 335     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            340                 345                 350         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        355                 360                 365             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    370                 375                 380                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
385                 390                 395                 400 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                405                 410                 415     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            420                 425                 430         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        435                 440                 445             


Ser Cys Ser Val Leu His Glu Ala Leu His Ser His Tyr Thr Gln Lys 
    450                 455                 460                 


Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470         


<210>  2
<211>  1416
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CHIKV-24 heavy chain ORF Sequence (Nucleotide)

<400>  2
auggaaaccg acacacugcu gcugugggug cugcuucuuu gggugcccgg aucuacagga       60

caggugcagc ugguugaauc uggcggcgga guugugcagc cuggcaaguc ucugagacug      120

agcugugccg ccagcggcuu caccuucaga aacuacggca ugcacugggu ccgacaggcu      180

ccaggcaaag gccuugauug ggucgcccug aucagcuacg acggcaccca caaguacuac      240

aaggacagcc ugaagggcag auucaccauc agccgggaca acuuccagaa caccguggac      300

cugcagauca acagccugag gccugacgac accgccgugu acuacugcgc caaagagcug      360

gcuacaagcg gcguggugga accucuggau ucuuggggac agggcacccu ggucacagug      420

ucuagcgccu cuacaaaggg acccagcgug uucccucugg cuccuagcag caagagcaca      480

agcggaggaa cagccgcucu gggcugucug gucaaggacu acuuucccga gccugugacc      540

guguccugga auucuggcgc ucugacaucc ggcgugcaca ccuuuccagc ugugcugcaa      600

agcagcggcc uguacucucu gagcagcguc gugacagugc caagcagcuc ucugggcacc      660

cagaccuaca ucugcaacgu gaaccacaag ccuagcaaca ccaaggugga caagaaggug      720

gaacccaaga gcugcgacaa gacccacacc uguccacccu guccugcucc agaacugcuc      780

ggcggaccuu ccguguuccu guuuccucca aagccuaagg acacccugau gaucagcaga      840

acacccgaag ugaccugcgu ggugguggac gugucucacg aggacccuga agugaaguuc      900

aauugguacg uggacggcgu ggaagugcac aacgccaaga ccaagccuag agaggaacag      960

uacaacagca ccuacagagu gguguccgug cugaccgugc ugcaccagga uuggcugaac     1020

ggcaaagagu acaagugcaa gguguccaac aaggcccugc cugcuccuau cgagaagacc     1080

aucagcaagg ccaagggcca gccuagggaa ccucaggugu acacacugcc uccaagcagg     1140

gacgagcuga ccaagaauca ggugucccug accugccucg ugaagggcuu cuacccuucc     1200

gauaucgccg uggaguggga gagcaacggc cagccugaga acaacuacaa gaccacuccu     1260

ccugugcugg acagcgacgg cucauucuuc cuguacagca agcugacagu ggacaagucc     1320

agguggcagc agggcaacgu guucagcugc agcgugcugc acgaagcccu gcacagccac     1380

uacacccaga agucccuguc ucugagcccu ggcaaa                               1416


<210>  3
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: CHIKV-24 light chain ORF Sequence (Amino Acid)

<400>  3

Met Glu Thr Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Thr Thr Gly Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser 
            20                  25                  30          


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser 
        35                  40                  45              


Leu Val Ser Ser Tyr Phe Gly Trp Tyr Gln Gln Lys Arg Gly Gln Ser 
    50                  55                  60                  


Pro Arg Leu Leu Ile Tyr Ala Ala Ser Thr Arg Ala Thr Gly Ile Pro 
65                  70                  75                  80  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                85                  90                  95      


Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Gly Asn Thr Pro Phe Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
        115                 120                 125             


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
    130                 135                 140                 


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
145                 150                 155                 160 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
                165                 170                 175     


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
            180                 185                 190         


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
        195                 200                 205             


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
    210                 215                 220                 


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235 


<210>  4
<211>  705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: CHIKV-24 light chain ORF Sequence (Nucleotide)

<400>  4
auggaaacac ccgcucagcu gcuguuccug cugcugcugu ggcugccuga uaccacaggc       60

gagaucgugc ugacacagag cccuggcaca cugucacugu cuccaggcga aagagccaca      120

cugagcugua gagccagcca gagccuggug uccagcuacu ucggcuggua ucagcagaag      180

agaggccagu cuccucggcu gcugaucuac gccgcuucua caagagccac cggcauuccc      240

gauagauuca gcggcucugg cagcggcacc gauuucaccc ugacaaucag cagacuggaa      300

cccgaggacu ucgccgugua cuacugucag caguacggca acacacccuu caccuuuggc      360

ggaggcacca agguggaaau caagagaaca guggcugcuc ccagcguguu caucuuccca      420

ccuuccgacg agcagcugaa gucuggcaca gccucugucg ugugccugcu gaacaacuuc      480

uacccucggg aagccaaggu gcaguggaag guggacaacg cccugcagag cggcaacagc      540

caagagagcg ugacagagca ggacagcaag gacuccaccu acagccugag cagcacacug      600

acccugagca aggccgacua cgagaagcac aagguguacg ccugcgaagu gacacaccag      660

ggccugucua gcccugugac caagagcuuc aacagaggcg agugc                      705


<210>  5
<211>  1592
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Construct Sequence

<400>  5
gggaaauaag agagaaaaga agaguaagaa gaaauauaag accccggcgc cgccaccaug       60

gaaaccgaca cacugcugcu gugggugcug cuucuuuggg ugcccggauc uacaggacag      120

gugcagcugg uugaaucugg cggcggaguu gugcagccug gcaagucucu gagacugagc      180

ugugccgcca gcggcuucac cuucagaaac uacggcaugc acuggguccg acaggcucca      240

ggcaaaggcc uugauugggu cgcccugauc agcuacgacg gcacccacaa guacuacaag      300

gacagccuga agggcagauu caccaucagc cgggacaacu uccagaacac cguggaccug      360

cagaucaaca gccugaggcc ugacgacacc gccguguacu acugcgccaa agagcuggcu      420

acaagcggcg ugguggaacc ucuggauucu uggggacagg gcacccuggu cacagugucu      480

agcgccucua caaagggacc cagcguguuc ccucuggcuc cuagcagcaa gagcacaagc      540

ggaggaacag ccgcucuggg cugucugguc aaggacuacu uucccgagcc ugugaccgug      600

uccuggaauu cuggcgcucu gacauccggc gugcacaccu uuccagcugu gcugcaaagc      660

agcggccugu acucucugag cagcgucgug acagugccaa gcagcucucu gggcacccag      720

accuacaucu gcaacgugaa ccacaagccu agcaacacca agguggacaa gaagguggaa      780

cccaagagcu gcgacaagac ccacaccugu ccacccuguc cugcuccaga acugcucggc      840

ggaccuuccg uguuccuguu uccuccaaag ccuaaggaca cccugaugau cagcagaaca      900

cccgaaguga ccugcguggu gguggacgug ucucacgagg acccugaagu gaaguucaau      960

ugguacgugg acggcgugga agugcacaac gccaagacca agccuagaga ggaacaguac     1020

aacagcaccu acagaguggu guccgugcug accgugcugc accaggauug gcugaacggc     1080

aaagaguaca agugcaaggu guccaacaag gcccugccug cuccuaucga gaagaccauc     1140

agcaaggcca agggccagcc uagggaaccu cagguguaca cacugccucc aagcagggac     1200

gagcugacca agaaucaggu gucccugacc ugccucguga agggcuucua cccuuccgau     1260

aucgccgugg agugggagag caacggccag ccugagaaca acuacaagac cacuccuccu     1320

gugcuggaca gcgacggcuc auucuuccug uacagcaagc ugacagugga caaguccagg     1380

uggcagcagg gcaacguguu cagcugcagc gugcugcacg aagcccugca cagccacuac     1440

acccagaagu cccugucucu gagcccuggc aaaugauaau aggcuggagc cucgguggcc     1500

uagcuucuug ccccuugggc cuccccccag ccccuccucc ccuuccugca cccguacccc     1560

cguggucuuu gaauaaaguc ugagugggcg gc                                   1592


<210>  6
<211>  881
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Construct Sequence

<400>  6
gggaaauaag agagaaaaga agaguaagaa gaaauauaag accccggcgc cgccaccaug       60

gaaacacccg cucagcugcu guuccugcug cugcuguggc ugccugauac cacaggcgag      120

aucgugcuga cacagagccc uggcacacug ucacugucuc caggcgaaag agccacacug      180

agcuguagag ccagccagag ccuggugucc agcuacuucg gcugguauca gcagaagaga      240

ggccagucuc cucggcugcu gaucuacgcc gcuucuacaa gagccaccgg cauucccgau      300

agauucagcg gcucuggcag cggcaccgau uucacccuga caaucagcag acuggaaccc      360

gaggacuucg ccguguacua cugucagcag uacggcaaca cacccuucac cuuuggcgga      420

ggcaccaagg uggaaaucaa gagaacagug gcugcuccca gcguguucau cuucccaccu      480

uccgacgagc agcugaaguc uggcacagcc ucugucgugu gccugcugaa caacuucuac      540

ccucgggaag ccaaggugca guggaaggug gacaacgccc ugcagagcgg caacagccaa      600

gagagcguga cagagcagga cagcaaggac uccaccuaca gccugagcag cacacugacc      660

cugagcaagg ccgacuacga gaagcacaag guguacgccu gcgaagugac acaccagggc      720

cugucuagcc cugugaccaa gagcuucaac agaggcgagu gcugauaaua ggcuggagcc      780

ucgguggccu agcuucuugc cccuugggcc uccccccagc cccuccuccc cuuccugcac      840

ccguaccccc guggucuuug aauaaagucu gagugggcgg c                          881


<210>  7

<400>  7
000

<210>  8

<400>  8
000

<210>  9

<400>  9
000

<210>  10

<400>  10
000

<210>  11

<400>  11
000

<210>  12

<400>  12
000

<210>  13
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR Sequence

<400>  13
gggaaauaag agagaaaaga agaguaagaa gaaauauaag accccggcgc cgccacc          57


<210>  14
<211>  119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3' UTR Sequence

<400>  14
ugauaauagg cuggagccuc gguggccuag cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccgu ggucuuugaa uaaagucuga gugggcggc       119


<210>  15
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 5' UTR v1.0

<400>  15
gggaaauaag agagaaaaga agaguaagaa gaaauauaag agccacc                     47


<210>  16
<211>  119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 3'UTR v1.0

<400>  16
ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc       60

cuccuccccu uccugcaccc guacccccgu ggucuuugaa uaaagucuga gugggcggc       119


