                         SEQUENCE LISTING

<110>  Synthetic Vector Designs LLC
       Luckow, Verne A.
 
<120>  Combinatorial Assembly of Ordered Composite Arrays of 
       Site-Specific Synthetic Transposons Inserted Into Sequences 
       Comprising Novel Target Sites in Stable Locations on Modular 
       Prokaryotic and Eukaryotic Vectors

<130>  950-951.013-WO

<150>  US 62/896,494
<151>  2019-09-05

<150>  US 62/906,003
<151>  2019-09-25

<150>  US 63/001,614
<151>  2020-03-30

<160>  240   

<170>  PatentIn version 3.5

<210>  1
<211>  14067
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(14067)
<223>  Transposon Tn7 (E. coli), Genbank Accession Number BM_NC_002525, 
       extracted from NC002525, comprising left and right ends of Tn7, 
       plus tnsABCDE in the right half, and int12, dhfr1, sat, aadA, 
       ybeA, ybfA, ybfB, and ybgA in the left half of the transposon.

<400>  1
tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat       60

ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa      120

ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact aatctagcta      180

acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt tcatcagtat      240

cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat cccgtatttc      300

cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta tcttgatgtg      360

gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta atatacagta      420

acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca ataataagtt      480

aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac tccgatctca      540

ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta ctccaagagc      600

ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag aaacgcacgc      660

aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac ctaagctctg      720

acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg aagtgtaatg      780

accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa caatggtaga      840

actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa aagtatcagg      900

ggctactcag ggctacataa agatatttat ctacttttac tgattgataa gtagcatcag      960

tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg tatagatttg     1020

cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat cacgccccgc     1080

ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac gcttgctaac     1140

gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt catgcagatg     1200

atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg gaaagacaaa     1260

catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa aaggcagcga     1320

tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct caatgagtta     1380

ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc cacccttacc     1440

gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca aacgcaagca     1500

ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt tgcgagtatc     1560

cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta gccgtctagg     1620

cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc tgttgtacaa     1680

aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc tgctgtttgc     1740

taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat gacgtttttt     1800

gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt tcagcgcata     1860

acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag acataaaacg     1920

ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt aaaaaagtgc     1980

ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa tgctaaataa     2040

attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca ggatagacgg     2100

catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta tttaatacaa     2160

gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg cttactaata     2220

aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta tatcgaagaa     2280

tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac agctcctgtt     2340

taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg aatcaatggg     2400

agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat ctgacaatga     2460

gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga aaataacgga     2520

tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc aagtagatac     2580

actacatata tctacaatag acatcgagcc ggaaggtgat gtttactttc ctgaaatccc     2640

cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt atagttacca     2700

aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc acgccttttg     2760

taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt catatgaaga     2820

tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat ttcattgttc     2880

gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc agaagtgtga     2940

gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct gcttgctatg     3000

gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca tggaacgatc     3060

tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga gtcgcgcaca     3120

gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc atacgattag     3180

agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc tttactctcg     3240

gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa acagcgatgt     3300

actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa gccgacaccg     3360

cttcgcggcg cggcttaatt caggagttaa acatcatgag ggaagcggtg atcgccgaag     3420

tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc     3480

tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg     3540

atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg     3600

accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca     3660

ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat     3720

ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca     3780

ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag     3840

cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa     3900

ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta     3960

cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg     4020

ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctaggc     4080

aggcttatct tggacaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg     4140

ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt     4200

caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagagagctg gggaagacta     4260

tgcgcgatct gttgaaggtg gttctaagcc tcgtacttgc gatggcatcg gggcaggcac     4320

ttgctgacct gccaattgtt ttagtggatg aagctcgtct tccctatgac tactccccat     4380

ccaactacga catttctcca agcaactacg acaactccat aagcaattac gacaatagtc     4440

catcaaatta cgacaactct gagagcaact acgataatag ttcatccaat tacgacaata     4500

gtcgcaacgg aaatcgtagg cttatatata gcgcaaatgg gtctcgcact ttcgccggct     4560

actacgtcat tgccaacaat gggacaacga acttcttttc cacatctggc aaaaggatgt     4620

tctacacccc aaaagggggg cgcggcgtct atggcggcaa agatgggagc ttctgcgggg     4680

cattggtcgt cataaatggc caattttcgc ttgccctgac agataacggc ctgaagatca     4740

tgtatctaag caactagcct gctctctaat aaaatgttag gcctcaacat ctagtcgcaa     4800

gctgagggga accactagtg tcatacgaac ctccaagaga cggttacaca aacgggtaca     4860

ttgttgatgt catgtatgac aatcgcccaa gtaagtatcc agctgtgttc agaacgtacg     4920

tccgaattcc agactatgtc gaactagata acgcggagga gctggccgac gcaatcattc     4980

gagaggttga gttaaaggag gcatggctca caagggtctt ggggaactca gccgatcctg     5040

acgcttctgt gggcaagtgg gaccggcgcc tagcgcagtg cggtggtatt gatgagatgt     5100

caagtggggt gcaggcaaca tacttcagtc caaacggtca gctggtcggc ttagtggcat     5160

acattgatgc cgaatggatg gatgaatagc tctgacgtta cgattcaggt gagacaacca     5220

gatgggcatt gcgtacaagc tcagttgcca ccgatgtggc tacgagtctg atcttcttta     5280

cctaggccaa ggtatggcga tgcttcctga gcaaatagtc ggaacgtgca catgctgcga     5340

caatctaacg acgatttcta taaatgaaag ctacggagta tgctcacttt gtggggctaa     5400

ggatcgtgtg atatttacaa gctatcagca tgaggcaagg ccaagaaatc cctgctatct     5460

agagagaagt ttcaaatatc aatgcccaag atgccatgcc ttcagtatgg agcctccgga     5520

tctaccggag atgctttggg actaatgctc cgctttgcct cgggtatccg ttcgacctat     5580

gtttaagagg aaacgcacgt gtctgccgcc aaagctcttg cctatgcact ttgtgacaag     5640

ttggaaagca aaaaagttga tgaagtcgta cgaaatcttg tttctagcgg ctggaatatt     5700

agggaagtgg cctgggaagc ttcgtccttt gatcatgaaa tgccctactt tgacacgggg     5760

cgggacttcg atattggatt tcctgacacg ctgtcatcca agattgagtt tttgaccgcg     5820

aatggatcaa tcgtccaagc acgtgctcgc ctgcacttca agcaggctct aatttttgcc     5880

aaggcgtcca aacatgtcgc cgcgctcaag aactcattgg attactacta tggatcgtct     5940

gtaatccccc ctcaaaacca cggaggctat ttgtagaaaa ttccattaat atgaactcaa     6000

ggtgttccct acggacgaat cagcgatgaa ggtggtgtac ctggctatcc aggcggcgac     6060

caagaaatgg acgatgccga tccgcaactg gaagccggcc atgaaccgtt ttatgatcga     6120

atttggtgac cgtttaaatg gccaccttta actgaccggc aaaagcactt acacaaaagg     6180

gtttacaggc tctttcagtc attcctttaa tgcgtaaatt gctctctaac cactctctca     6240

gcccaccctt ccatatcctc tctcgatact tgcagctccc ccaacccctt tggatgattc     6300

acccctctat gcccgtcttg tccatataat tgatcaaaca aactatttgg ccaattcaat     6360

gagctcttca ccactccacg ccgtatcttt tcaaaatcat tccgccatgt ttcgctatca     6420

acgccactca acactttggt agaaagcatt ttcaccccat ccgatgcatc cacctctagc     6480

aatacaaatt cactgccatt acgttgcacc ccaacggctt taatcactct tggtgagcca     6540

tctttacaca gatgtaatcg gctacgccca acttttggca aaaccaaggt ttcttcaaaa     6600

agcacccgac atgcaaattt agtttttaga attgaaagta gctcatcaaa agcagcaaat     6660

cgattagcaa aaatagagtt gtaattggtt gcatcctgct tcccacccac atctgccgct     6720

gctaggacac cacctaagtg tggctcatca gtactaacca aattactatt ttcacttgtt     6780

gttgcttctt ctttttctaa aatagtttgg ctactttttc ttgccctgcg agatttttca     6840

attcgactag gtctactaaa acttatccat gtcggctcag cctctatgac tcgtgtgtct     6900

gtgtctatat tggctgtttc ctcatcgtcc aactgtagat gctcatcatc aacaactgac     6960

tctgttgaaa ccgctatgtg ctgcgtacta ccatcacctg cctccttttc ctgaaaagag     7020

gcatggctaa tagctgttgt gctaggaagc atagcgttga tttctaaacc tactatttcc     7080

tcaactaaat aatccttatc ctcgttagaa gaacgtcctt ttacatgtaa tttccaaccc     7140

tgcatgggcg gagggtcaaa gctaaagcac cagctttcaa cgccgttctt aatttctcta     7200

ttttgttgat agtgcctaaa aatactttca tacgaatcca taacatcttg atccgaaaac     7260

aaccaaacca aaagctgcac tacggccgac tgctctaacg cccctttagg aaacgatgaa     7320

ctgggtaaga tccttatctc taaatgatct cgctcaacct catactgaac gtcgaactct     7380

tgctgtaaag cggtactgct caaacagctt cgacagaaat aggagttaat taaaaataat     7440

gaccgagcta gctctaactg tgggatgtga taacgagtct tgctatatgg tgtttcagct     7500

tcgtaaataa atgaactctg cgctccatcc ttgttgcgta cggaagcaaa ggacgagaga     7560

ttaggaaaat cgattatttt ggctcgttga agatccgatg cttcaaactc aatcacacga     7620

tccgccttat ttatttgctt tgttgtactg ttaaccacct tagctcgact taataaaggc     7680

aaatgagtaa atcgagtcca tttccgttct tgcattggat taaaccaaac aaaaatacgc     7740

cactccttat gacccgagtt acggaataaa tgaccaatat gtacaacctg cacattgtca     7800

ttaaatgtag ctagcctaac cactcactct tccccataaa ccatttccaa gaatctttgt     7860

gcttcctcag ttatccgctc tttagataac gttgcacttc ttaataatcg ccagcgccta     7920

agctcaacat cttcctgttt aagcttaata aaagcttggc taatccgtct aatttgataa     7980

tcttccacac tctctgagta acgctttaag caaagcgcta ccaaaggcag tttctgtaga     8040

ttttttgcaa gagaggttcc gttaggagtt tgctttaaca gccagctcga tgtcgctctt     8100

gggtgatcaa ggctactatc tagacgcttt atgattctta atagttgtcg tacagcaatt     8160

cgatctcttt ggttccaatc aactctaggg gcgggtgcca gacgctcttg ttgatgctgt     8220

tgattccagt gaactagcca atccctgtca tgtcggtaaa gccaagcgta tagcacccca     8280

ccctctaaag actgtcttgc cgccttaatt ccttggtatt tatgcactag ttgctgccag     8340

tctttatgct taacagataa atcttcagag ttaggttgca cagactggct aacaggtctt     8400

gtcgttatag agtgctcagt aagagcactt gcctgctgta gcgcttctat aaccgttagt     8460

tttggcaata aggcttgcca cacaatacta tgctgtaaat aactaaaggc ttttctatgc     8520

ttacggaata tacttttcag ccaacacgtg tccttgttct ctgccaactt taaatccagt     8580

ttctctagtg cctcatcact aaaagtctgc ctcactctct ccgccaccaa gtcatgacga     8640

atgtgcttgc ttttggttag ccctagatcc tgcgctaagc gctgataaaa cagcgtccac     8700

tgctcaaggc ttggggaaag ctcttgcgct cgtggagcat ctaacagagg ggctatataa     8760

gcagctagtg ctgttaattg agatagggag tctttggggt agtctgaaag cagctcagta     8820

tgacccaaag cccaaaattg atgtcggtga tcatctacag ctctatcaaa gaagactaaa     8880

gcaccgtgtt ttggacaata tggcaaagcg ggcaaatacc aatctcgttg ccaaaaggct     8940

tccccatacc tatttagctg aagagcaacg caatcagggc agtatctaaa gcggttatcg     9000

ctcttaactc tagaagcagc gactcctagc attaaatgca ccgcaccttg cgcttggtac     9060

tccattaacc gaatagcttc gtctcggcgc tccttgccta caaacggagc atataaaggg     9120

aataaggtat gctcataaat aagctgctga acagcgtaac gtcctgtttg atgtagatgt     9180

cttgctatca cacctaaatg cgagggcaga cctaaggtag cgaccacctt gcggttgcca     9240

tacacctcat ccaacagctg cttaggacta acaatccctt gataaacgcc tgcccgtgca     9300

atagtgctat aaatcagctc attcgagtac ggaacaggaa agtttctcat ggctaacctg     9360

cttgtttaaa taagctcgcc atatccacta ttaccccttt cccttttaac cgttcatgca     9420

tggttttttc aggttggcgt tgtgaataga tataacgtaa atccgtatca ggcaagctat     9480

cccagtcgct gggcttgact acctttatag ccgaaacctt tttactcttg gagggctttt     9540

ctagttctga cactaccgtt tcgccttcca tcaaccactg caaaacaaga ggcagtaact     9600

tttgtcttgt catcgttgga ttctggctaa acgctttttt aatagtggga attaacaggc     9660

ttgaatcgta atcctctttc agcatcagat ataaatgacg ctgatcttcg gtatctaact     9720

cttgaagggc tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct agctgaagtt     9780

ggattaaccg tttatcaatc tcgggaacga ctagatcaga ataacgagca atgcgttctg     9840

ggatacccga gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca tcttgataca     9900

cttgccgcaa taaaccagcg gtaatacgct cattgcctaa agctagcgca cggagctgag     9960

cgagtacaaa aagttttact acaatgtcca tcactccttg gcttagctca taccacacat    10020

cacggacctc atccgataac agcgcatctt tgcgttgtaa aagctgtaat tgccaaagat    10080

tatccgtaaa agcgatccac tcttgattgg gctttccacg ttgcgtttgt tgtataggat    10140

cccagaatat agctccaaac cctgcccctc tacgtgcaga ccgcaaatca gcctcaaaaa    10200

tctctcgtgc tttaggggta ccaatcaaca tcactggtac gccaataata ttcaccatcg    10260

tcacaaaaaa gttcagcatc tcttgagatc cacccgaacg agagcggctt aaatgctgaa    10320

tttcatcaat aaccaacaac cctaaagcat gtgcattggc tatttgcgac atcaaagcca    10380

acatggtttc tataccatga cgttttaagc cataacgacg ctcatagttc gagcccaagg    10440

ctcgatccaa cgctctgaaa aaattcaagc agatttcttt tagcgaacca ttatgcgagc    10500

agtctatttt caaatacacc acctgctcta cattgagttc acgatggtaa atcacctgag    10560

gatacgtggc tagaatacga tgaagagagg tcgtcttccc actaccagaa caaccaatta    10620

acaataagct ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc aactctcccg    10680

tttgaacacg ctcataacca ttttgtaaat gcttttgtaa atctcctgtt ttaggatttc    10740

tgcctacgta gccacctcga atcatgaccg aaatacgctc acttagtagc aaatgcgtac    10800

ctaatggctg aaaatagtca tctggaatac gacaaatggt atgagctctg ataacacggg    10860

acttttgcaa gtcagaggaa gtaagctgta aagaggattt cagtgatgca gcactattca    10920

ctgactcttg taatggtggt aaggcctcga taaaaggatt atcacgataa gcctctaccc    10980

ccgtatcacg ataaactgct tgaatccggg tagcactcat gactcatcct tttctggtgg    11040

atcctgaaat aattcaggca cgtatgtggg taggctgtaa tcttcttgat catccgcttc    11100

cactgcgttg aaaggaataa ctttagcctc atcacctgaa gagcttggct tcaaatgctc    11160

cgcacgtttt ttacgctccg aggtcacggc ttctttttta ttagttttaa tctgcttaat    11220

gcgtgttgat ttgggctcag tagtactggg cgttaactta ttcgctttct gaatggtttg    11280

ctgaataaac gcctcaagct ccctgcgttt agttaactca tcctgcttcg cattggcttt    11340

attgtgtttt tcttgtgctt gtatatccca aacctcccaa aatgagagac ctttaaactg    11400

ccgactacgt tccgtcagat tacagcgcca aaatacacgg ctgccaactt gcggaaacaa    11460

ataaatcgta tcaaccagca ctgggtcata agccgcttct aaatgttgag gtctagctat    11520

atcagtgctc cgctgcaacc aaccctcacg cagaatctct gaccccgagt aatacaaacc    11580

ccacaaatta acgccaaatg aagaaataga gacctttcgg cgaggcagta acgctactcg    11640

caactgctct tgctccacag cccttaaact acctgtacga tgctgcatac cccattgcca    11700

tagctggaca ggaatagacg gtaaatctgt aggaaaatca gcatctcgat cgtatttatc    11760

catcaccaga tggttatttc tgaataagat cgtacgcaaa ataatttgtg tgaactcaaa    11820

taccgacaga gatgcatcta acctatagtc tgtttcacca tggcttttga tccgactgcc    11880

ctctacaatg ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa aagtgctttc    11940

cactatgcct ttagcatcgc cacgtctagg tggagcactt tccactcgca cattaaaact    12000

agaaactaag gcttcgacct gatgactcat taattcgcca cggtccgcta gcaacacatc    12060

tggcaaacct acacacggcc agtctgagct actaatctca atatcatgct gggcacaaat    12120

ggccgttttg tcagagcaag catttacaaa agcctgcatc gccaccacat aagacggatt    12180

ttcaaagccg atataaaagc ccgtgatcat ccgactaaac acatcaatca caatgtaaag    12240

cgttggtctt cctatgattt tttggcgatc atgatgatcc actaaataaa tatcagcaat    12300

cgtggcatca atctcataac gactcccagg gcctaacgcc tgagaagtgg ctgtactact    12360

taagggtcgt acgtcttttt tatatacccc tgctttaact ctagacttta agcgctgagc    12420

tttagggtat tctcgatcat aaaaataacg aaactgacgt agtgttgggt aatcctcttg    12480

gggaatgcga ggaaaatact gagcaaacaa gtccacaaat cgtctatagg caacggtggt    12540

ctttgtacct ttttgattta acaggtgctt ttctatggtc aacctaaaaa ggcgttcaat    12600

ctcgggcgtt accttggttc cttcaccctt accatattct ctggctcggc caatctttgc    12660

tgttcctgtc gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt caggaattaa    12720

tgcattaggc gtttgaccac gctgccagta acggcgtaac aacttataaa ctgtagcctt    12780

agtaacctta tgttcttgga ccacatgctc aacgagttcg cttctgactt tagggtcgaa    12840

acgatcctta ctattaataa taggaagaat ttttcgataa tcctcctcgc gcttctggaa    12900

gctgacagaa tctacagacg gctcttctaa atcaagatgt acataagggt catcagttct    12960

aactaagcgg ccttcatcta aatactgcat tagcaacaac tcagccctag cttgtggaac    13020

tcctttatca gcgcttattt gcatccagac aacttggcca tcctctatag ccaaaatgcg    13080

atacggatca ttatcaaata gcacaacctc attaatttgc cacatagcgc aactcctcca    13140

tattcactac ttggctaata cagagatctg cacacttatt tgccctgaaa gacttataaa    13200

tattgaactt aataaaacca tttgcggtta aggctcgtat ctcactcaat gttttgccta    13260

actccaaatc ataagcaata tcaacctgct tacagacatt gataatgttt tcatctcctt    13320

tttcttgcag gatatgggcc aatggggata gttgtgctaa aagctccgca gaaacttctt    13380

ctgttttcac tgaataaagc cattcaatat tttcttttac tacgggattt atttctttat    13440

cagtaaaaat gaaccaagga atttgctttt gctgccaata gcgacgctct agttctagtt    13500

tttctaaggt acgctcgtct tgtaaggctg ctgcaggttt gacttgaata gcaaactgct    13560

caaaaggacc atctttgcag tccactaaaa aatcagtaga cataacctga tctacaccac    13620

gaataacagg atgcttaata ccactatcta ttgcaatctg cctggtatca ctaggtaata    13680

aggggaactg ctcgcgtata tctagcacgc tgctctccca ctcaagactg agaaaaacag    13740

caagctctaa gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa taaatacggt    13800

gggaacgacc tgaagaagga acttcttgta ctgttagcca tggaatatag tctttaccat    13860

gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt    13920

ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca    13980

gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc    14040

agtttaagac tttattgtcc gcccaca                                        14067


<210>  2
<211>  61
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(61)
<223>  Sequences extending from -2, -1, 0, +1 +2, and +3 to +58 of the 
       attachment site for Tn7 near the E. coli glmS gene, where 
       positions -2 to +2 are duplicated as 5-bp sequences at both ends 
       of a Tn7 element after transposition into this sequence.

<400>  2
cccgcttacg cagggcatcc atttattact caaccgtaac cgattttgcc aggttacgcg       60

g                                                                       61


<210>  3
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Junction of 5-bp duplication near Tn7L inserted between positions
       -2 to +2 of attTn7 near 3' end of E. coli glmS gene.


<220>
<221>  misc_feature
<222>  (1)..(13)
<223>  Junction of 5- duplication when Tn7 inserts betweenn positions -2
       to +2 of attTn7L near 3' end of E. coli glmS gene.

<220>
<221>  misc_feature
<222>  (1)..(13)
<223>  Junction of 5-bp duplication when Tn7 inserts betweenn positions 
       -2 to +2 of attTn7L near 3' end of E. coli glmS gene.

<400>  3
cccgctgtgg gcg                                                          13


<210>  4
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Junction of 5-bp duplication near Tn7R inserted between positions
       -2 to +2 of attTn7 near 3' end of E. coli glmS gene.


<220>
<221>  misc_feature
<222>  (1)..(69)
<223>  Junction of 5-bp duplication when Tn7 inserts betweenn positions 
       -2 to +2 of attTn7R near 3' end of E. coli glmS gene.

<400>  4
cgcccacacc cgcttacgca gggcatccat ttattactca accgtaaccg attttgccag       60

gttacgcgg                                                               69


<210>  5
<211>  549
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic lacZ-alpha-mini-attTn7 sequence.

<400>  5
atgaccatga ttacgccaag ctcggaatta accctcacta aagggaacaa aagctggagc       60

tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcacat      120

aacaggaaga aaaatgcccc gcttacgcag ggcatccatt tattactcaa ccgtaaccga      180

ttttgccagg ttacgcggct gtcgacctcg agggggggcc cggtacccaa ttcgccctat      240

agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac      300

cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat      360

agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg      420

gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cttcgaagac      480

gctcgacagc gacacacttg catcggatgc agcccggtta acgtgccggc acggcctggg      540

taaccaggt                                                              549


<210>  6
<211>  266
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic truncated lacZalpha-mini-attTn7

<400>  6
atgaccatga ttacgaattc acataacagg aagaaaaatg ccccgcttac gcagggcatc       60

catttattac tcaaccgtaa ccgattttgc caggttacgc ggctgtcgac ttcactggcc      120

gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca      180

gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc      240

caacagttgc gcagcctgaa tggtaa                                           266


<210>  7
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites.


<220>
<221>  CDS
<222>  (1)..(33)
<223>  Polypeptide sequence encoded at carboxy terminal region of Type I
       CAT protein, represented by QYCDEWQGGA*

<400>  7
cag tac tgc gat gag tgg cag ggc ggg gcg taa tttttttaag gcagttattg       53
Gln Tyr Cys Asp Glu Trp Gln Gly Gly Ala                                   
1               5                   10                                    

gtgcccgggc ttaaacgcct ggt                                               76


<210>  8
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Gln Tyr Cys Asp Glu Trp Gln Gly Gly Ala 
1               5                   10  


<210>  9
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TAA stop codon.

<400>  9
cagtactgct aagagtggca gggcggggcg taattttttt aaggcagtta ttggtgcccg       60

ggcttaaacg cctggt                                                       76


<210>  10
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TGA, stop codon.

<400>  10
cagtactgct gagagtggca gggcggggcg taattttttt aaggcagtta ttggtgcccg       60

ggcttaaacg cctggt                                                       76


<210>  11
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TAG stop codon.

<400>  11
cagtactgct aggagtggca gggcggggcg taattttttt aaggcagtta ttggtgcccg       60

ggcttaaacg cctggt                                                       76


<210>  12
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' end of the Type I cat gene, adding SrfI and XmaI sites, before
       changing the GAT to a TAA, TGA, or TAG stop codon, and adding an 
       overlapping mini-attTn7 site


<220>
<221>  misc_feature
<222>  (1)..(100)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of the Type I cat gene, adding SrfI and XmaI sites, before 
       changing the GAT to a TAA, TGA, or TAG stop codon, and adding an 
       mini-attTn7 site overlapping with the stop codon

<400>  12
cagtactgcg atcttacgca gggcatccat ttattactca accgtaaccg attttgccag       60

gttacgcggc tgtcgacgtg cccgggctta aacgcctggt                            100


<210>  13
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TAA stop codon, and adding an overlapping 
       mini-attTn7 site.


<220>
<221>  misc_feature
<222>  (1)..(100)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TAA stop codon, and adding an mini-attTn7 site 
       overlapping with the stop codon

<400>  13
cagtactgct aacttacgca gggcatccat ttattactca accgtaaccg attttgccag       60

gttacgcggc tgtcgacgtg cccgggctta aacgcctggt                            100


<210>  14
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TGA, stop codon, and adding an overlapping 
       mini-attTn7 site.


<220>
<221>  misc_feature
<222>  (1)..(100)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TGA  stop codon, and adding an mini-attTn7 site 
       overlapping with the stop codon

<400>  14
cagtactgct gacttacgca gggcatccat ttattactca accgtaaccg attttgccag       60

gttacgcggc tgtcgacgtg cccgggctta aacgcctggt                            100


<210>  15
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TAG stop codon, and adding an overlapping 
       mini-attTn7 site.


<220>
<221>  misc_feature
<222>  (1)..(100)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TAA, TGA, or TAG stop codon, and adding an 
       mini-attTn7 site overlapping with the stop codon

<400>  15
cagtactgct agcttacgca gggcatccat ttattactca accgtaaccg attttgccag       60

gttacgcggc tgtcgacgtg cccgggctta aacgcctggt                            100


<210>  16
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of Type I cat gene, adding SrfI and XmaI sites, changing the 
       TGC to a TAA, TGA, or TAG stop codon, and the GAT to a TAA stop 
       codon, adding mini-attTn7 overlapping with the first stop codon


<220>
<221>  misc_feature
<222>  (1)..(93)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of Type I cat gene, adding SrfI and XmaI sites, changing the 
       TGC to a TAA, TGA, or TAG stop codon, and the GAT to a TAA stop 
       codon, adding mini-attTn7 overlapping with the first stop codon

<400>  16
cagtactgcg atagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc       60

ggctgtcgac gtgcccgggc ttaaacgcct ggt                                    93


<210>  17
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of Type I cat gene, adding SrfI and XmaI sites, changing the 
       TGC to a TAA stop codon, and the GAT to a TAA stop codon, adding 
       mini-attTn7 overlapping with the first stop codon


<220>
<221>  misc_feature
<222>  (1)..(93)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of Type I cat gene, adding SrfI and XmaI sites, changing the 
       TGC to a TAA stop codon, and the GAT to a TAA stop codon, adding 
       mini-attTn7 overlapping with the first stop codon

<400>  17
cagtactaat aaagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc       60

ggctgtcgac gtgcccgggc ttaaacgcct ggt                                    93


<210>  18
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TAG stop codon, and adding a long mini-attTn7 site.


<220>
<221>  misc_feature
<222>  (1)..(93)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of Type I cat gene, adding SrfI and XmaI sites, changing the 
       TGC to a TGA stop codon, and the GAT to a TAA stop codon, adding 
       mini-attTn7 overlapping with the first stop codon

<400>  18
cagtactgat aaagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc       60

ggctgtcgac gtgcccgggc ttaaacgcct ggt                                    93


<210>  19
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences From the Tat/ScaI site to the BaeGI/Bme1508I at the 3' 
       end of the Type I cat gene, adding SrfI and XmaI sites, changing 
       the GAT to a TGA stop codon, and adding a long mini-attTn7 site.


<220>
<221>  misc_feature
<222>  (1)..(93)
<223>  Sequences From the TatI/ScaI site to the BaeGI/Bme1508I at the 3'
       end of Type I cat gene, adding SrfI and XmaI sites, changing the 
       TGC to a TAG stop codon, and the GAT to a TAA stop codon, adding 
       mini-attTn7 overlapping with the first stop codon

<400>  19
cagtactagt aaagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc       60

ggctgtcgac gtgcccgggc ttaaacgcct ggt                                    93


<210>  20
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences at the 3' end of a Type I cat gene after transposition 
       of a mini-Tn7 into an over overlapping mini-attTn7 site.


<220>
<221>  CDS
<222>  (3)..(38)
<223>  Polypeptide sequences 3' end of a Type I cat gene after 
       transposition of a mini-Tn7 into an over overlapping mini-attTn7 
       site.

<400>  20
gc ttt att aat cgg cta caa gag ttg cgt tat gtg ggc g                    39
   Phe Ile Asn Arg Leu Gln Glu Leu Arg Tyr Val Gly                        
   1               5                   10                                 


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  21

Phe Ile Asn Arg Leu Gln Glu Leu Arg Tyr Val Gly 
1               5                   10          


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  3' end of Tn7R after transposition an over overlapping 
       mini-attTn7 site

<400>  22
cgcccacatg ttattacgca gg                                                22


<210>  23
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' end of Type I cat gene to mimic insertion of Tn7L replacing 
       stop codon for Cys codon


<220>
<221>  misc_feature
<222>  (1)..(67)
<223>  Sequences at the 3' end of a Type I cat gene that mimic Tn7L at 
       the junction of mini-Tn7 replacing a stop codon for a Cys codon 
       in an overlapping mini-attTn7 site

<220>
<221>  CDS
<222>  (1)..(24)
<223>  Polypeptide sequence that mimics insertion of the Tn7L replacing 
       the stop codon for a Cys codon, restoring activity to the encoded
       CAT fusion protein

<400>  23
cag tac tgt ggg cgg aca aaa tag ggggcgtaat ttttttaagg atcctattgg        54
Gln Tyr Cys Gly Arg Thr Lys                                               
1               5                                                         

tgcccgggct gcc                                                          67


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  24

Gln Tyr Cys Gly Arg Thr Lys 
1               5           


<210>  25
<211>  180
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(180)
<223>  5' end of E. coli lacZ gene nucleotides 1-180

<400>  25
atg acc atg att acg gat tca ctg gcc gtc gtt tta caa cgt cgt gac         48
Met Thr Met Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp           
1               5                   10                  15                

tgg gaa aac cct ggc gtt acc caa ctt aat cgc ctt gca gca cat ccc         96
Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro           
            20                  25                  30                    

cct ttc gcc agc tgg cgt aat agc gaa gag gcc cgc acc gat cgc cct        144
Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro           
        35                  40                  45                        

tcc caa cag ttg cgc agc ctg aat ggc gaa tgg cgc                        180
Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg                           
    50                  55                  60                            


<210>  26
<211>  60
<212>  PRT
<213>  Escherichia coli

<400>  26

Met Thr Met Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp 
1               5                   10                  15      


Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro 
            20                  25                  30          


Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro 
        35                  40                  45              


Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg 
    50                  55                  60  


<210>  27
<211>  57
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(57)
<223>  5' end of lacZ delta M15 gene of E. coli enoding amino acids 1-11
       and 42 -49

<400>  27
atg acc atg att acg gat tca ctg gcc gtc gtg gcc cgc acc gat cgc         48
Met Thr Met Ile Thr Asp Ser Leu Ala Val Val Ala Arg Thr Asp Arg           
1               5                   10                  15                

cct tcc caa                                                             57
Pro Ser Gln                                                               
                                                                          


<210>  28
<211>  19
<212>  PRT
<213>  Escherichia coli

<400>  28

Met Thr Met Ile Thr Asp Ser Leu Ala Val Val Ala Arg Thr Asp Arg 
1               5                   10                  15      


Pro Ser Gln 
            


<210>  29
<211>  360
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacZ alpha gene with MCS region pUC19 from positions 1-360


<220>
<221>  CDS
<222>  (1)..(324)
<223>  LacZ alpha gene with MCS region pUC19 from positions 1-360

<400>  29
atg acc atg att acg cca agc ttg cat gcc tgc agg tcg act cta gag         48
Met Thr Met Ile Thr Pro Ser Leu His Ala Cys Arg Ser Thr Leu Glu           
1               5                   10                  15                

gat ccc cgg gta ccg agc tcg aat tca ctg gcc gtc gtt tta caa cgt         96
Asp Pro Arg Val Pro Ser Ser Asn Ser Leu Ala Val Val Leu Gln Arg           
            20                  25                  30                    

cgt gac tgg gaa aac cct ggc gtt acc caa ctt aat cgc ctt gca gca        144
Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala           
        35                  40                  45                        

cat ccc cct ttc gcc agc tgg cgt aat agc gaa gag gcc cgc acc gat        192
His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp           
    50                  55                  60                            

cgc cct tcc caa cag ttg cgc agc ctg aat ggc gaa tgg cgc ctg atg        240
Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Leu Met           
65                  70                  75                  80            

cgg tat ttt ctc ctt acg cat ctg tgc ggt att tca cac cgc ata tgg        288
Arg Tyr Phe Leu Leu Thr His Leu Cys Gly Ile Ser His Arg Ile Trp           
                85                  90                  95                

tgc act ctc agt aca atc tgc tct gat gcc gca tag ttaagccagc             334
Cys Thr Leu Ser Thr Ile Cys Ser Asp Ala Ala                               
            100                 105                                       

cccgacaccc gccaacaccc gctgac                                           360


<210>  30
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  30

Met Thr Met Ile Thr Pro Ser Leu His Ala Cys Arg Ser Thr Leu Glu 
1               5                   10                  15      


Asp Pro Arg Val Pro Ser Ser Asn Ser Leu Ala Val Val Leu Gln Arg 
            20                  25                  30          


Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala 
        35                  40                  45              


His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp 
    50                  55                  60                  


Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Leu Met 
65                  70                  75                  80  


Arg Tyr Phe Leu Leu Thr His Leu Cys Gly Ile Ser His Arg Ile Trp 
                85                  90                  95      


Cys Thr Leu Ser Thr Ile Cys Ser Asp Ala Ala 
            100                 105         


<210>  31
<211>  260
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(186)
<223>  Sequences from 1-260 of the lacZ gene, but polypetide sequence 
       diverges areound nucleotide 186 compared to those in pUC19

<400>  31
atg acc atg att acg gat tca ctg gcc gtc gtt tta caa cgt cgt gac         48
Met Thr Met Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp           
1               5                   10                  15                

tgg gaa aac cct ggc gtt acc caa ctt aat cgc ctt gca gca cat ccc         96
Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro           
            20                  25                  30                    

cct ttc gcc agc tgg cgt aat agc gaa gag gcc cgc acc gat cgc cct        144
Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro           
        35                  40                  45                        

tcc caa cag ttg cgc agc ctg aat ggc gaa tgg cgc ttt gcc                186
Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala                   
    50                  55                  60                            

tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc      246

gatactgtcg tcgt                                                        260


<210>  32
<211>  62
<212>  PRT
<213>  Escherichia coli

<400>  32

Met Thr Met Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp 
1               5                   10                  15      


Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro 
            20                  25                  30          


Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro 
        35                  40                  45              


Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala 
    50                  55                  60          


<210>  33
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19


<220>
<221>  CDS
<222>  (1)..(120)

<400>  33
acc caa ctt aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt         48
Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg           
1               5                   10                  15                

aat agc gaa gag gcc cgc acc gat cgc cct tcc caa cag ttg cgc agc         96
Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg Ser           
            20                  25                  30                    

ctg aat ggc gaa tgg cgc ctg atg                                        120
Leu Asn Gly Glu Trp Arg Leu Met                                           
        35                  40                                            


<210>  34
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  34

Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg 
1               5                   10                  15      


Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg Ser 
            20                  25                  30          


Leu Asn Gly Glu Trp Arg Leu Met 
        35                  40  


<210>  35
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding NS


<220>
<221>  CDS
<222>  (1)..(54)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding NS

<400>  35
acc caa ctt aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt         48
Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg           
1               5                   10                  15                

taa taa gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg         104

cgaatggcgc ctgatg                                                      120


<210>  36
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  36

Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg 
1               5                   10                  15      


<210>  37
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding SE


<220>
<221>  CDS
<222>  (1)..(57)

<400>  37
acc caa ctt aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt         48
Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg           
1               5                   10                  15                

aat taa taa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc                 97
Asn                                                                       
                                                                          

tgaatggcga atggcgcctg atg                                              120


<210>  38
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  38

Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg 
1               5                   10                  15      


Asn 
    


<210>  39
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding EE


<220>
<221>  CDS
<222>  (1)..(60)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding EE

<400>  39
acc caa ctt aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt         48
Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg           
1               5                   10                  15                

aat agc taa taa gcccgcaccg atcgcccttc ccaacagttg cgcagcctga            100
Asn Ser                                                                   
                                                                          

atggcgaatg gcgcctgatg                                                  120


<210>  40
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  40

Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg 
1               5                   10                  15      


Asn Ser 
        


<210>  41
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding EA


<220>
<221>  CDS
<222>  (1)..(63)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding EA

<400>  41
acc caa ctt aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt         48
Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg           
1               5                   10                  15                

aat agc gaa taa taa cgcaccgatc gcccttccca acagttgcgc agcctgaatg        103
Asn Ser Glu                                                               
                                                                          

gcgaatggcg cctgatg                                                     120


<210>  42
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  42

Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg 
1               5                   10                  15      


Asn Ser Glu 
            


<210>  43
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding AR


<220>
<221>  CDS
<222>  (1)..(66)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with 
       synthetic oligonucleotides comprising two TAA stop codons near 
       codons encoding AR

<400>  43
acc caa ctt aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt         48
Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg           
1               5                   10                  15                

aat agc gaa gag taa taa accgatcgcc cttcccaaca gttgcgcagc                96
Asn Ser Glu Glu                                                           
            20                                                            

ctgaatggcg aatggcgcct gatg                                             120


<210>  44
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  44

Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg 
1               5                   10                  15      


Asn Ser Glu Glu 
            20  


<210>  45
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19


<220>
<221>  CDS
<222>  (1)..(84)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19

<400>  45
gcc agc tgg cgt aat agc gaa gag gcc cgc acc gat cgc cct tcc caa         48
Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln           
1               5                   10                  15                

cag ttg cgc agc ctg aat ggc gaa tgg cgc ctg atg                         84
Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Leu Met                           
            20                  25                                        


<210>  46
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  46

Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln 
1               5                   10                  15      


Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Leu Met 
            20                  25              


<210>  47
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing SE codons


<220>
<221>  misc_feature
<222>  (1)..(84)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing SE codons

<400>  47
gccagctggc gttaataaga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc       60

ctgaatggcg aatggcgcct gatg                                              84


<210>  48
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing NS codons


<220>
<221>  misc_feature
<222>  (1)..(84)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing NS codons

<400>  48
gccagctggc gtaattaata agaggcccgc accgatcgcc cttcccaaca gttgcgcagc       60

ctgaatggcg aatggcgcct gatg                                              84


<210>  49
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing EE codons

<400>  49
gccagctggc gtaatagcta ataagcccgc accgatcgcc cttcccaaca gttgcgcagc       60

ctgaatggcg aatggcgcct gatg                                              84


<210>  50
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing EA codons


<220>
<221>  misc_feature
<222>  (1)..(84)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing EE codons

<400>  50
gccagctggc gtaatagcga ataataacgc accgatcgcc cttcccaaca gttgcgcagc       60

ctgaatggcg aatggcgcct gatg                                              84


<210>  51
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing AR codons


<220>
<221>  misc_feature
<222>  (1)..(84)
<223>  PvuII to KasI sites of LacZ alpha gene pUC18 or pUC19 with stop 
       codons replacing EE codons

<400>  51
gccagctggc gtaatagcga agagtaataa accgatcgcc cttcccaaca gttgcgcagc       60

ctgaatggcg aatggcgcct gatg                                              84


<210>  52
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic mini-attTn7 from -2 to +2 with nunknown nucleotides at 
       the insertion site, followed by +3 to +58, then Synthetic SalI, 
       KasI and other restriction sites


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  The 5 "n" residues from positions 1-5 each represent ANY 
       nucleotde residue in the attachment site for Tn7 (attTn7) that 
       are part of a 5-bp sequence that is duplicated at both ends of 
       Tn7 after transposition.

<220>
<221>  misc_feature
<222>  (6)..(85)
<223>  A mini-attTn7 target site from +3 to + 58 followed by synthetic 
       SalI, KasI and other restriction sites

<400>  52
nnnnnttacg cagggcatcc atttattact caaccgtaac cgattttgcc aggttacgcg       60

gctgtcgacg tgcccgggcg gcgcc                                             85


<210>  53
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences near double stop codons replacing EA codons in 
       lacZalpha peptide after transposition of a mini-Tn7 into an 
       overlapping mini-attTn7 site


<220>
<221>  misc_feature
<222>  (1)..(43)
<223>  Seqiemces from -2 to  +41 of a mini-attTn7, including zero 
       position between -2 and +2

<400>  53
aagagttacg cagggcatcc atttattact caaccgtaac cga                         43


<210>  54
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Junction near target site after transptotion into TAA stop codon 
       reading frame +1

<400>  54
agcgaatgtg ggcg                                                         14


<210>  55
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Junction near target site after transptotion into TAA stop codon 
       reading frame +1

<400>  55
agcgaattgt gggcg                                                        15


<210>  56
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Junction near target site after transptotion into TAA stop codon 
       reading frame +3

<400>  56
agcgaatatg tgggcg                                                       16


<210>  57
<211>  381
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18 lacZalpha region containing an EcoRI-SalI fragment from 
       bMON14272 comprising a mini-attTn7 fragment


<220>
<221>  CDS
<222>  (1)..(381)
<223>  Chimareic fusion protein comprising lacZalpha fragment with 
       insertion of EcoRI-SalI fragment comprising a synthetic 
       mini-attTn7 fragment

<400>  57
atg acc atg att acg aat tca cat aac agg aag aaa aat gcc ccg ctt         48
Met Thr Met Ile Thr Asn Ser His Asn Arg Lys Lys Asn Ala Pro Leu           
1               5                   10                  15                

acg cag ggc atc cat tta tta ctc aac cgt aac cga ttt tgc cag gtt         96
Thr Gln Gly Ile His Leu Leu Leu Asn Arg Asn Arg Phe Cys Gln Val           
            20                  25                  30                    

acg cgg ctg tcg acc tgc agg cat gca agc ttg gca ctg gcc gtc gtt        144
Thr Arg Leu Ser Thr Cys Arg His Ala Ser Leu Ala Leu Ala Val Val           
        35                  40                  45                        

tta caa cgt cgt gac tgg gaa aac cct ggc gtt acc caa ctt aat cgc        192
Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg           
    50                  55                  60                            

ctt gca gca cat ccc cct ttc gcc agc tgg cgt aat agc gaa gag gcc        240
Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala           
65                  70                  75                  80            

cgc acc gat cgc cct tcc caa cag ttg cgc agc ctg aat ggc gaa tgg        288
Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp           
                85                  90                  95                

cgc ctg atg cgg tat ttt ctc ctt acg cat ctg tgc ggt att tca cac        336
Arg Leu Met Arg Tyr Phe Leu Leu Thr His Leu Cys Gly Ile Ser His           
            100                 105                 110                   

cgc ata tgg tgc act ctc agt aca atc tgc tct gat gcc gca tag            381
Arg Ile Trp Cys Thr Leu Ser Thr Ile Cys Ser Asp Ala Ala                   
        115                 120                 125                       


<210>  58
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  58

Met Thr Met Ile Thr Asn Ser His Asn Arg Lys Lys Asn Ala Pro Leu 
1               5                   10                  15      


Thr Gln Gly Ile His Leu Leu Leu Asn Arg Asn Arg Phe Cys Gln Val 
            20                  25                  30          


Thr Arg Leu Ser Thr Cys Arg His Ala Ser Leu Ala Leu Ala Val Val 
        35                  40                  45              


Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg 
    50                  55                  60                  


Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala 
65                  70                  75                  80  


Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp 
                85                  90                  95      


Arg Leu Met Arg Tyr Phe Leu Leu Thr His Leu Cys Gly Ile Ser His 
            100                 105                 110         


Arg Ile Trp Cys Thr Leu Ser Thr Ile Cys Ser Asp Ala Ala 
        115                 120                 125     


<210>  59
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences near the unique PstI site in the beta lactamase gene of
       pACYC177


<220>
<221>  CDS
<222>  (1)..(60)
<223>  Sequences near unique PstI site in pACYC177

<400>  59
cca aac gac gag cgt gac acc acg atg cct gca gca atg gca aca acg         48
Pro Asn Asp Glu Arg Asp Thr Thr Met Pro Ala Ala Met Ala Thr Thr           
1               5                   10                  15                

ttg cgc aaa cta                                                         60
Leu Arg Lys Leu                                                           
            20                                                            


<210>  60
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  60

Pro Asn Asp Glu Arg Asp Thr Thr Met Pro Ala Ala Met Ala Thr Thr 
1               5                   10                  15      


Leu Arg Lys Leu 
            20  


<210>  61
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  paCYC177 with unique PistI site mutated to EagI site


<220>
<221>  misc_feature
<222>  (1)..(60)
<223>  Sequences near unique PstI site in pACYC177 mutated to EagI site

<400>  61
ccaaacgacg agcgtgacac cacgatgccg gccgcaatgg caacaacgtt gcgcaaacta       60


<210>  62
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177 with unique PstIsite mutated  to unique PvuII site


<220>
<221>  misc_feature
<222>  (1)..(60)
<223>  Sequences near unique PstI site mutated to unique PvuII site

<400>  62
ccaaacgacg agcgtgacac cacgatgcca gctgcaatgg caacaacgtt gcgcaaacta       60


<210>  63
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177 with PstI site near the 3' end of the NPT-II gene that 
       don't change the amino acids "LQ" encoded by the wild-type gene


<220>
<221>  CDS
<222>  (1)..(48)
<223>  Sequences near 3' end of the NPT-II gene encoding a protein 
       conferring resitance to kanamycin

<400>  63
gat atg aat aaa ttg cag ttt cat ttg atg ctc gat gag ttt ttc taa         48
Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe               
1               5                   10                  15                

tcagaattgg tt                                                           60


<210>  64
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  64

Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
1               5                   10                  15  


<210>  65
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177 with PstI site near 3' end of NPT-II gene that do not 
       change the amino acids encoded at that position


<220>
<221>  CDS
<222>  (1)..(48)
<223>  Sequences near 3' end of pACYC177 with a new PstI site that don't
       change amino acids "LQ" encoded at that postion in the NPT-II 
       gene

<400>  65
gat atg aat aaa ttg cag ttt cat ttg atg ctc gat gag ttt ttc taa         48
Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe               
1               5                   10                  15                

tcagaattgg tt                                                           60


<210>  66
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  66

Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
1               5                   10                  15  


<210>  67
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKM2 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(21)
<223>  pKM2 3' end of NPT-II gene

<400>  67
ctt ctt gac gag ttc ttc tga gcgggactct ggggttcgaa atgaccacca            51
Leu Leu Asp Glu Phe Phe                                                   
1               5                                                         


<210>  68
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  68

Leu Leu Asp Glu Phe Phe 
1               5       


<210>  69
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKM243 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(27)
<223>  pKM243 3' end of NPT-II gene

<400>  69
ctt ctt gac gag ttc ttc caa gct taa                                     27
Leu Leu Asp Glu Phe Phe Gln Ala                                           
1               5                                                         


<210>  70
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  70

Leu Leu Asp Glu Phe Phe Gln Ala 
1               5               


<210>  71
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKM243/1 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(18)
<223>  pKM243/1 3' end of NPT-II gene

<400>  71
ctt ctt gac gag ttc ttc                                                 18
Leu Leu Asp Glu Phe Phe                                                   
1               5                                                         


<210>  72
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  72

Leu Leu Asp Glu Phe Phe 
1               5       


<210>  73
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKM143-1 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(51)
<223>  pKM243-1 3' end pf NPT-II gene

<400>  73
ctt ctt gac gag ttc ttc cca agc ttt aat gcg gta gtt tat cac agt         48
Leu Leu Asp Glu Phe Phe Pro Ser Phe Asn Ala Val Val Tyr His Ser           
1               5                   10                  15                

taa                                                                     51


<210>  74
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  74

Leu Leu Asp Glu Phe Phe Pro Ser Phe Asn Ala Val Val Tyr His Ser 
1               5                   10                  15      


<210>  75
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(21)
<223>  pACYC177 3' end of NPT-II gene

<400>  75
atg ctc gat gag ttt ttc taa tcagaattgg ttaattggtt gt                    43
Met Leu Asp Glu Phe Phe                                                   
1               5                                                         


<210>  76
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  76

Met Leu Asp Glu Phe Phe 
1               5       


<210>  77
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-QA 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(27)
<223>  pACYC177-QA 3' end of NPT-II gene

<400>  77
atg ctc gat gag ttt ttc caa gct taa ttggttaatt ggttgt                   43
Met Leu Asp Glu Phe Phe Gln Ala                                           
1               5                                                         


<210>  78
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  78

Met Leu Asp Glu Phe Phe Gln Ala 
1               5               


<210>  79
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-PS 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(27)
<223>  pACYC177-PS 3' end of NPT-II gene

<400>  79
atg ctc gat gag ttt ttc cca agc taa ttggttaatt ggttgt                   43
Met Leu Asp Glu Phe Phe Pro Ser                                           
1               5                                                         


<210>  80
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  80

Met Leu Asp Glu Phe Phe Pro Ser 
1               5               


<210>  81
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-PSFNAVVYHS 3' end of NPT-II gene


<220>
<221>  CDS
<222>  (1)..(51)
<223>  pACYC177-PSFNAVVYHS 3' end of NPT-II gene

<400>  81
atg ctc gat gag ttt ttc cca agc ttt aat gcg gta gtt tat cac agt         48
Met Leu Asp Glu Phe Phe Pro Ser Phe Asn Ala Val Val Tyr His Ser           
1               5                   10                  15                

taa                                                                     51


<210>  82
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  82

Met Leu Asp Glu Phe Phe Pro Ser Phe Asn Ala Val Val Tyr His Ser 
1               5                   10                  15      


<210>  83
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-Q** with two TAA stop codons after Q codon


<220>
<221>  CDS
<222>  (1)..(27)
<223>  pACYC177-Q** with two stop codons after a Q codon

<400>  83
atg ctc gat gag ttt ttc caa taa taa ttggttaatt ggttgt                   43
Met Leu Asp Glu Phe Phe Gln                                               
1               5                                                         


<210>  84
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  84

Met Leu Asp Glu Phe Phe Gln 
1               5           


<210>  85
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-P** with two TAA stop codons after a P codon


<220>
<221>  CDS
<222>  (1)..(27)
<223>  pACYC177-PS with two TAA stop codons after a P codon

<400>  85
atg ctc gat gag ttt ttc cca taa taa ttggttaatt ggttgt                   43
Met Leu Asp Glu Phe Phe Pro                                               
1               5                                                         


<210>  86
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  86

Met Leu Asp Glu Phe Phe Pro 
1               5           


<210>  87
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177 3' end of beta-lactamase gene


<220>
<221>  CDS
<222>  (2)..(28)
<223>  pACYC177 3' end of beta lactamase gene

<400>  87
a ggt gcc tca ctg att aag cat tgg taa ctgtcagacc aagtttactc at          50
  Gly Ala Ser Leu Ile Lys His Trp                                         
  1               5                                                       


<210>  88
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  88

Gly Ala Ser Leu Ile Lys His Trp 
1               5               


<210>  89
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-K*** with two TAA stop codons befoe the normal TAA stop 
       codon


<220>
<221>  CDS
<222>  (2)..(25)
<223>  pACYC177-K*** with two TAA stop codons befoe the normal TAA stop 
       codon

<400>  89
a ggt gcc tca ctg att aag taa taa taactgtcag accaagttta ctcat           50
  Gly Ala Ser Leu Ile Lys                                                 
  1               5                                                       


<210>  90
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  90

Gly Ala Ser Leu Ile Lys 
1               5       


<210>  91
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-KH**


<220>
<221>  CDS
<222>  (2)..(28)
<223>  pACYC177-KH** with two stop codons after KH, one replacing 
       "essential Tryptophan (W) codon

<400>  91
a ggt gcc tca ctg att aag cat taa taa ctgtcagacc aagtttactc at          50
  Gly Ala Ser Leu Ile Lys His                                             
  1               5                                                       


<210>  92
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  92

Gly Ala Ser Leu Ile Lys His 
1               5           


<210>  93
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-KWH**


<220>
<221>  CDS
<222>  (2)..(31)
<223>  pACYC177-KHW** with two stop codons at site of normal TAA stop 
       codon

<400>  93
a ggt gcc tca ctg att aag cat tgg taa taa tcagaccaag tttactcat          50
  Gly Ala Ser Leu Ile Lys His Trp                                         
  1               5                                                       


<210>  94
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  94

Gly Ala Ser Leu Ile Lys His Trp 
1               5               


<210>  95
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAG

<400>  95
aagtgtgggc g                                                            11


<210>  96
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAGT

<400>  96
aagttgtggg cg                                                           12


<210>  97
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAGTA

<400>  97
aagtatgtgg gcg                                                          13


<210>  98
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAGCAT

<400>  98
aagcattgtg ggcg                                                         14


<210>  99
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAGCATTT

<400>  99
aagcatttgt gggcg                                                        15


<210>  100
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAGCATTA

<400>  100
aagcattatg tgggcg                                                       16


<210>  101
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAGCATTGG

<400>  101
aagcattggt gtgggcg                                                      17


<210>  102
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC1770AAGCATTGGT

<400>  102
aagcattggt tgtgggcg                                                     18


<210>  103
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-AAGCATTGGTA

<400>  103
aagcattggt atgtgggcg                                                    19


<210>  104
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACUC177-PstI-BglI spanning junction between alpha and omega 
       fragments of beta-lactamase


<220>
<221>  CDS
<222>  (1)..(141)
<223>  pACUC177-PstI-BglI spanning junction between alpha and omega 
       fragments of beta-lactamase

<400>  104
atg cct gca gca atg gca aca acg ttg cgc aaa cta tta act ggc gaa         48
Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu           
1               5                   10                  15                

cta ctt act cta gct tcc cgg caa caa tta ata gac tgg atg gag gcg         96
Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp Met Glu Ala           
            20                  25                  30                    

gat aaa gtt gca gga cca ctt ctg cgc tcg gcc ctt ccg gct ggc            141
Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro Ala Gly               
        35                  40                  45                        


<210>  105
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  105

Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu 
1               5                   10                  15      


Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp Met Glu Ala 
            20                  25                  30          


Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro Ala Gly 
        35                  40                  45          


<210>  106
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-Pst-AseI with linker


<220>
<221>  CDS
<222>  (1)..(105)
<223>  pACYC177-PstI-AseI with synthetic linker at junction of alpha and
       omega fragments of beta lactamase

<400>  106
atg cct gca gca atg gca aca acg ttg cgc aaa cta tta act ggc gaa         48
Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu           
1               5                   10                  15                

acg aat tcg cgg ccg cgt cga cgt cta ctt act cta gct tcc cgg caa         96
Thr Asn Ser Arg Pro Arg Arg Arg Leu Leu Thr Leu Ala Ser Arg Gln           
            20                  25                  30                    

caa tta ata                                                            105
Gln Leu Ile                                                               
        35                                                                


<210>  107
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  107

Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu 
1               5                   10                  15      


Thr Asn Ser Arg Pro Arg Arg Arg Leu Leu Thr Leu Ala Ser Arg Gln 
            20                  25                  30          


Gln Leu Ile 
        35  


<210>  108
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC177-bla-alpha-omega-mini-attTn7


<220>
<221>  CDS
<222>  (1)..(180)
<223>  pACYC177-bla-alpha-omega-mini-attTn7 with mini-attTn7 at the 
       junction of the alpha and omega peptides of beta-lactamase

<400>  108
atg cct gca gca atg gca aca acg ttg cgc aaa cta tta act ggc gaa         48
Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu           
1               5                   10                  15                

acg aat tca cat aac agg aag aaa aat gcc ccg ctt acg cag ggc atc         96
Thr Asn Ser His Asn Arg Lys Lys Asn Ala Pro Leu Thr Gln Gly Ile           
            20                  25                  30                    

cat tta tta ctc aac cgt aac cga ttt tgc cag gtt acg cgg gtc gac        144
His Leu Leu Leu Asn Arg Asn Arg Phe Cys Gln Val Thr Arg Val Asp           
        35                  40                  45                        

cgt cta ctt act cta gct tcc cgg caa caa tta ata                        180
Arg Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile                           
    50                  55                  60                            


<210>  109
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  109

Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu 
1               5                   10                  15      


Thr Asn Ser His Asn Arg Lys Lys Asn Ala Pro Leu Thr Gln Gly Ile 
            20                  25                  30          


His Leu Leu Leu Asn Arg Asn Arg Phe Cys Gln Val Thr Arg Val Asp 
        35                  40                  45              


Arg Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile 
    50                  55                  60  


<210>  110
<211>  401
<212>  PRT
<213>  Escherichia coli


<220>
<221>  DOMAIN
<222>  (181)..(207)
<223>  Interdomain loop in Tn10 tetracycline resistance protein 
       ETKNTRDNTDTEVGVETQSNSVYITLF

<400>  110

Met Asn Ser Ser Thr Lys Ile Ala Leu Val Ile Thr Leu Leu Asp Ala 
1               5                   10                  15      


Met Gly Ile Gly Leu Ile Met Pro Val Leu Pro Thr Leu Leu Arg Glu 
            20                  25                  30          


Phe Ile Ala Ser Glu Asp Ile Ala Asn His Phe Gly Val Leu Leu Ala 
        35                  40                  45              


Leu Tyr Ala Leu Met Gln Val Ile Phe Ala Pro Trp Leu Gly Lys Met 
    50                  55                  60                  


Ser Asp Arg Phe Gly Arg Arg Pro Val Leu Leu Leu Ser Leu Ile Gly 
65                  70                  75                  80  


Ala Ser Leu Asp Tyr Leu Leu Leu Ala Phe Ser Ser Ala Leu Trp Met 
                85                  90                  95      


Leu Tyr Leu Gly Arg Leu Leu Ser Gly Ile Thr Gly Ala Thr Gly Ala 
            100                 105                 110         


Val Ala Ala Ser Val Ile Ala Asp Thr Thr Ser Ala Ser Gln Arg Val 
        115                 120                 125             


Lys Trp Phe Gly Trp Leu Gly Ala Ser Phe Gly Leu Gly Leu Ile Ala 
    130                 135                 140                 


Gly Pro Ile Ile Gly Gly Phe Ala Gly Glu Ile Ser Pro His Ser Pro 
145                 150                 155                 160 


Phe Phe Ile Ala Ala Leu Leu Asn Ile Val Thr Phe Leu Val Val Met 
                165                 170                 175     


Phe Trp Phe Arg Glu Thr Lys Asn Thr Arg Asp Asn Thr Asp Thr Glu 
            180                 185                 190         


Val Gly Val Glu Thr Gln Ser Asn Ser Val Tyr Ile Thr Leu Phe Lys 
        195                 200                 205             


Thr Met Pro Ile Leu Leu Ile Ile Tyr Phe Ser Ala Gln Leu Ile Gly 
    210                 215                 220                 


Gln Ile Pro Ala Thr Val Trp Val Leu Phe Thr Glu Asn Arg Phe Gly 
225                 230                 235                 240 


Trp Asn Ser Met Met Val Gly Phe Ser Leu Ala Gly Leu Gly Leu Leu 
                245                 250                 255     


His Ser Val Phe Gln Ala Phe Val Ala Gly Arg Ile Ala Thr Lys Trp 
            260                 265                 270         


Gly Glu Lys Thr Ala Val Leu Leu Gly Phe Ile Ala Asp Ser Ser Ala 
        275                 280                 285             


Phe Ala Phe Leu Ala Phe Ile Ser Glu Gly Trp Leu Val Phe Pro Val 
    290                 295                 300                 


Leu Ile Leu Leu Ala Gly Gly Gly Ile Ala Leu Pro Ala Leu Gln Gly 
305                 310                 315                 320 


Val Met Ser Ile Gln Thr Lys Ser His Gln Gln Gly Ala Leu Gln Gly 
                325                 330                 335     


Leu Leu Val Ser Leu Thr Asn Ala Thr Gly Val Ile Gly Pro Leu Leu 
            340                 345                 350         


Phe Ala Val Ile Tyr Asn His Ser Leu Pro Ile Trp Asp Gly Trp Ile 
        355                 360                 365             


Trp Ile Ile Gly Leu Ala Phe Tyr Cys Ile Ile Ile Leu Leu Ser Met 
    370                 375                 380                 


Thr Phe Met Leu Thr Pro Gln Ala Gln Gly Ser Lys Gln Glu Thr Ser 
385                 390                 395                 400 


Ala 
    


<210>  111
<211>  396
<212>  PRT
<213>  Shigella flexneri


<220>
<221>  DOMAIN
<222>  (183)..(209)
<223>  Interdomain loop in pACYC184 tetraccyline gene indrectly derived 
       from pSC101 isolated from Shigella flexneri 
       ESHKGERRPMPLRAFNPVSSFRWARGM

<400>  111

Met Lys Ser Asn Asn Ala Leu Ile Val Ile Leu Gly Thr Val Thr Leu 
1               5                   10                  15      


Asp Ala Val Gly Ile Gly Leu Val Met Pro Val Leu Pro Gly Leu Leu 
            20                  25                  30          


Arg Asp Ile Val His Ser Asp Ser Ile Ala Ser His Tyr Gly Val Leu 
        35                  40                  45              


Leu Ala Leu Tyr Ala Leu Met Gln Phe Leu Cys Ala Pro Val Leu Gly 
    50                  55                  60                  


Ala Leu Ser Asp Arg Phe Gly Arg Arg Pro Val Leu Leu Ala Ser Leu 
65                  70                  75                  80  


Leu Gly Ala Thr Ile Asp Tyr Ala Ile Met Ala Thr Thr Pro Val Leu 
                85                  90                  95      


Trp Ile Leu Tyr Ala Gly Arg Ile Val Ala Gly Ile Thr Gly Ala Thr 
            100                 105                 110         


Gly Ala Val Ala Gly Ala Tyr Ile Ala Asp Ile Thr Asp Gly Glu Asp 
        115                 120                 125             


Arg Ala Arg His Phe Gly Leu Met Ser Ala Cys Phe Gly Val Gly Met 
    130                 135                 140                 


Val Ala Gly Pro Val Ala Gly Gly Leu Leu Gly Ala Ile Ser Leu His 
145                 150                 155                 160 


Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn Leu Leu Leu 
                165                 170                 175     


Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Arg Arg Pro Met 
            180                 185                 190         


Pro Leu Arg Ala Phe Asn Pro Val Ser Ser Phe Arg Trp Ala Arg Gly 
        195                 200                 205             


Met Thr Ile Val Ala Ala Leu Met Thr Val Phe Phe Ile Met Gln Leu 
    210                 215                 220                 


Val Gly Gln Val Pro Ala Ala Leu Trp Val Ile Phe Gly Glu Asp Arg 
225                 230                 235                 240 


Phe Arg Trp Ser Ala Thr Met Ile Gly Leu Ser Leu Ala Val Phe Gly 
                245                 250                 255     


Ile Leu His Ala Leu Ala Gln Ala Phe Val Thr Gly Pro Ala Thr Lys 
            260                 265                 270         


Arg Phe Gly Glu Lys Gln Ala Ile Ile Ala Gly Met Ala Ala Asp Ala 
        275                 280                 285             


Leu Gly Tyr Val Leu Leu Ala Phe Ala Thr Arg Gly Trp Met Ala Phe 
    290                 295                 300                 


Pro Ile Met Ile Leu Leu Ala Ser Gly Gly Ile Gly Met Pro Ala Leu 
305                 310                 315                 320 


Gln Ala Met Leu Ser Arg Gln Val Asp Asp Asp His Gln Gly Gln Leu 
                325                 330                 335     


Gln Gly Ser Leu Ala Ala Leu Thr Ser Leu Thr Ser Ile Ile Gly Pro 
            340                 345                 350         


Leu Ile Val Thr Ala Ile Tyr Ala Ala Ser Ala Ser Thr Trp Asn Gly 
        355                 360                 365             


Leu Ala Trp Ile Val Gly Ala Ala Leu Tyr Leu Val Cys Leu Pro Ala 
    370                 375                 380                 


Leu Arg Arg Gly Ala Trp Ser Arg Ala Thr Ser Thr 
385                 390                 395     


<210>  112
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC184 reverse complement spanning Tet Iinterdomain Loop


<220>
<221>  CDS
<222>  (1)..(210)
<223>  Sequence from the reverse complement of pACYC184 flanking the 
       interdomain loop of the tetracycline resistance protein

<400>  112
tcc ttg cat gca cca ttc ctt gcg gcg gcg gtg ctc aac ggc ctc aac         48
Ser Leu His Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn           
1               5                   10                  15                

cta cta ctg ggc tgc ttc cta atg cag gag tcg cat aag gga gag cgt         96
Leu Leu Leu Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Arg           
            20                  25                  30                    

cga ccg atg ccc ttg aga gcc ttc aac cca gtc agc tcc ttc cgg tgg        144
Arg Pro Met Pro Leu Arg Ala Phe Asn Pro Val Ser Ser Phe Arg Trp           
        35                  40                  45                        

gcg cgg ggc atg act atc gtc gcc gca ctt atg act gtc ttc ttt atc        192
Ala Arg Gly Met Thr Ile Val Ala Ala Leu Met Thr Val Phe Phe Ile           
    50                  55                  60                            

atg caa ctc gta gga cag                                                210
Met Gln Leu Val Gly Gln                                                   
65                  70                                                    


<210>  113
<211>  70
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  113

Ser Leu His Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn 
1               5                   10                  15      


Leu Leu Leu Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Arg 
            20                  25                  30          


Arg Pro Met Pro Leu Arg Ala Phe Asn Pro Val Ser Ser Phe Arg Trp 
        35                  40                  45              


Ala Arg Gly Met Thr Ile Val Ala Ala Leu Met Thr Val Phe Phe Ile 
    50                  55                  60                  


Met Gln Leu Val Gly Gln 
65                  70  


<210>  114
<211>  297
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC184 reverse complement Tet-mini-attTn7


<220>
<221>  CDS
<222>  (1)..(297)
<223>  pACYC184 reverse complement Tet-mini-attTn7, with synthetic 
       mini-attTn7 inserted near SalI site in the sequences encoding the
       interdomain linker of the tetracycline resistance protein

<400>  114
tcc ttg cat gca cca ttc ctt gcg gcg gcg gtg ctc aac ggc ctc aac         48
Ser Leu His Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn           
1               5                   10                  15                

cta cta ctg ggc tgc ttc cta atg cag gag tcg cat aag gga gag aat         96
Leu Leu Leu Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Asn           
            20                  25                  30                    

tca cat aac agg aag aaa aat gcc ccg ctt acg cag ggc atc cat tta        144
Ser His Asn Arg Lys Lys Asn Ala Pro Leu Thr Gln Gly Ile His Leu           
        35                  40                  45                        

tta ctc aac cgt aac cga ttt tgc cag gtt acg cgg cgt cga ccg atg        192
Leu Leu Asn Arg Asn Arg Phe Cys Gln Val Thr Arg Arg Arg Pro Met           
    50                  55                  60                            

ccc ttg aga gcc ttc aac cca gtc agc tcc ttc cgg tgg gcg cgg ggc        240
Pro Leu Arg Ala Phe Asn Pro Val Ser Ser Phe Arg Trp Ala Arg Gly           
65                  70                  75                  80            

atg act atc gtc gcc gca ctt atg act gtc ttc ttt atc atg caa ctc        288
Met Thr Ile Val Ala Ala Leu Met Thr Val Phe Phe Ile Met Gln Leu           
                85                  90                  95                

gta gga cag                                                            297
Val Gly Gln                                                               
                                                                          


<210>  115
<211>  99
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  115

Ser Leu His Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn 
1               5                   10                  15      


Leu Leu Leu Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Asn 
            20                  25                  30          


Ser His Asn Arg Lys Lys Asn Ala Pro Leu Thr Gln Gly Ile His Leu 
        35                  40                  45              


Leu Leu Asn Arg Asn Arg Phe Cys Gln Val Thr Arg Arg Arg Pro Met 
    50                  55                  60                  


Pro Leu Arg Ala Phe Asn Pro Val Ser Ser Phe Arg Trp Ala Arg Gly 
65                  70                  75                  80  


Met Thr Ile Val Ala Ala Leu Met Thr Val Phe Phe Ile Met Gln Leu 
                85                  90                  95      


Val Gly Gln 
            


<210>  116
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  An EcoRI-SalI fragment comrpising a synthetic mini-attTn7


<220>
<221>  misc_feature
<222>  (1)..(95)
<223>  EcoRI-SalI fragment comprising a synthetic mini-attTn7

<400>  116
gaattcacat aacaggaaga aaaatgcccc gcttacgcag ggcatccatt tattactcaa       60

ccgtaaccga ttttgccagg ttacgcggcg tcgac                                  95


<210>  117
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheitc Not-PspOMI linker


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  Synthetic linker comprising NotI and PspOMI sites GC'GGCC,GC 
       nnnnnnnn G'GGCC,C

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  N repreasents any nucleotide

<400>  117
gcggccgcnn nnnnnngggc cc                                                22


<210>  118
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Linker with NotI-scar-PspOMI sites


<220>
<221>  misc_feature
<222>  (1)..(37)
<223>  Synthetic linker with NotI-scar-PspOMI sites GC'GGCC,GC nnnnnnnn 
       GGGCCGC nnnnnnnn G'GGCC,C

<220>
<221>  misc_feature
<222>  (9)..(16)
<223>  N repreasents any nucleotide

<220>
<221>  misc_feature
<222>  (24)..(31)
<223>  N repreasents any nucleotide

<400>  118
gcggccgcnn nnnnnngggc cgcnnnnnnn ngggccc                                37


<210>  119
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PspOMI-NotI linker


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  Synthetic PspOMI-NotI linker

<400>  119
gggcccnnnn nnnngcggcc gc                                                22


<210>  120
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic PspOMI-scar-NotI linker


<220>
<221>  misc_feature
<222>  (1)..(37)
<223>  Synthetic PspOMI-scar--NotI linker

<400>  120
gggcccnnnn nnnngcggcc cnnnnnnnng cggccgc                                37


<210>  121
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheitc AbsI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AbsI-SgrDI linker

<400>  121
cctcgaggnn nnnnnncgtc gacg                                              24


<210>  122
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AbsI-scar-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(40)
<223>  Synthetic AbsI-scar-SgrDI linker

<400>  122
cctcgaggnn nnnnnncgtc gaggnnnnnn nncgtcgacg                             40


<210>  123
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic SgrDI-AbsI linker

<400>  123
cgtcgacgnn nnnnnncctc gagg                                              24


<210>  124
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-scar-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(40)
<223>  Synthetic SgrDI-scar-AbsI linker

<400>  124
cgtcgacgnn nnnnnncctc gacgnnnnnn nncctcgagg                             40


<210>  125
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic MauBI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic MauBI-AscI linker

<400>  125
cgcgcgcgnn nnnnnnggcg cgcc                                              24


<210>  126
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic MauBI-scar-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(40)
<223>  Synthetic MauBI-scar-AscI linker

<400>  126
cgcgcgcgnn nnnnnnggcg cgcgnnnnnn nnggcgcgcc                             40


<210>  127
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AscI-MauBI linker

<400>  127
ggcgcgccnn nnnnnncgcg cgcg                                              24


<210>  128
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-scar-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(40)
<223>  Synthetic AscI-scar-MauBI linker

<400>  128
ggcgcgccnn nnnnnncgcg cgccnnnnnn nncgcgcgcg                             40


<210>  129
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic MauBI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic MauBI-AbsI linker

<400>  129
cgcgcgcgnn nnnnnncctc gagg                                              24


<210>  130
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheitc MauBI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Syntheitc MauBI-SgrDI linker

<400>  130
cgcgcgcgnn nnnnnncgtc gacg                                              24


<210>  131
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AscI-AbsI linker

<400>  131
ggcgcgccnn nnnnnncctc gagg                                              24


<210>  132
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthteit AscI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthteit AscI-SgrDI linker

<400>  132
ggcgcgccnn nnnnnncgtc gacg                                              24


<210>  133
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AbsI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AbsI-MauBI linker

<400>  133
cctcgaggnn nnnnnncgcg cgcg                                              24


<210>  134
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AbsI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AbsI-AscI linker

<400>  134
cctcgaggnn nnnnnnggcg cgcc                                              24


<210>  135
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic SgrDI-MauBI linker

<400>  135
cgtcgacgnn nnnnnncgcg cgcg                                              24


<210>  136
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synhtetic SgrDI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Syntheitc SgrDI-AscI linker

<400>  136
cgtcgacgnn nnnnnnggcg cgcc                                              24


<210>  137
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic MauBI-PacI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic MauBI-PacI-AbsI linker

<400>  137
cgcgcgcgtt aattaacctc gagg                                              24


<210>  138
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheitc MauBI-PacI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Syntheitc MauBI-PacI-SgrDI linker

<400>  138
cgcgcgcgtt aattaacgtc gacg                                              24


<210>  139
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-PacI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AscI-PacI-AbsI linker

<400>  139
ggcgcgcctt aattaacctc gagg                                              24


<210>  140
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-PacI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AscI-PacI-SgrDI linker

<400>  140
ggcgcgcctt aattaacgtc gacg                                              24


<210>  141
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AbsI-PacI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AbsI-PacI-MauBI linker

<400>  141
cctcgaggtt aattaacgcg cgcg                                              24


<210>  142
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AbsI-PacI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AbsI-PacI-AscI linker

<400>  142
cctcgaggtt aattaaggcg cgcc                                              24


<210>  143
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-PacI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic SgrDI-PacI-MauBI linker

<400>  143
cgtcgacgtt aattaacgcg cgcg                                              24


<210>  144
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-PacI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  SgrDI-PacI-AscI linker

<400>  144
cgtcgacgtt aattaaggcg cgcc                                              24


<210>  145
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic MauBI-PacI-AbsI-AvrII-SgrDI-PacI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Synthetic MauBI-PacI-AbsI-AvrII-SgrDI-PacI-AscI linker

<400>  145
cgcgcgcgtt aattaacctc gaggcctagg cgtcgacgtt aattaaggcg cgcc             54


<210>  146
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MauBI-PacI-SgrDI-AvrII-AbsI-PacI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  MauBI-PacI-SgrDI-AvrII-AbsI-PacI-AscI linker

<400>  146
cgcgcgcgtt aattaacgtc gacgcctagg cctcgaggtt aattaaggcg cgcc             54


<210>  147
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-PacI-AbsI-AvrII-SgrDI-PacI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Synthetic AscI-PacI-AbsI-AvrII-SgrDI-PacI-MauBI linker

<400>  147
ggcgcgcctt aattaacctc gaggcctagg cgtcgacgtt aattaacgcg cgcg             54


<210>  148
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-PacI-SgrDI-AvrII-AbsI-PacI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Synthetic AscI-PacI-SgrDI-AvrII-AbsI-PacI-MauBI linker

<400>  148
ggcgcgcctt aattaacgtc gacgcctagg cctcgaggtt aattaacgcg cgcg             54


<210>  149
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AbsI-PacI-MauBI-AvrII-AscI-PacI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Synthetic AbsI-PacI-MauBI-AvrII-AscI-PacI-SgrDI linker

<400>  149
cctcgaggtt aattaacgcg cgcgcctagg ggcgcgcctt aattaacgtc gacg             54


<210>  150
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AbsI-PacI-AscI-AvrII-MauBI-PacI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Synthetic AbsI-PacI-AscI-AvrII-MauBI-PacI-SgrDI linker

<400>  150
cctcgaggtt aattaaggcg cgcccctagg cgcgcgcgtt aattaacgtc gacg             54


<210>  151
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-PacI-MauBI-AvrII-AscI-PacI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Synthetic SgrDI-PacI-MauBI-AvrII-AscI-PacI-AbsI linker

<400>  151
cgtcgacgtt aattaacgcg cgcgcctagg ggcgcgcctt aattaacctc gagg             54


<210>  152
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-PacI-AscI-AvrII-MauBI-PacI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Synthetic SgrDI-PacI-AscI-AvrII-MauBI-PacI-AbsI linker

<400>  152
cgtcgacgtt aattaaggcg cgcccctagg cgcgcgcgtt aattaacctc gagg             54


<210>  153
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Syntheitc MauBI-PacI-AscI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Syntheitc MauBI-PacI-AscI linker

<400>  153
cgcgcgcgtt aattaaggcg cgcc                                              24


<210>  154
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic AscI-PacI-MauBI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AscI-PacI-MauBI linker

<400>  154
ggcgcgcctt aattaacgcg cgcg                                              24


<210>  155
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synteitc AscI-PacI-SgrDI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Synthetic AscI-PacI-SgrDI linker

<400>  155
cctcgaggtt aattaacgtc gacg                                              24


<210>  156
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic SgrDI-PacI-AbsI linker


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Syntehitc SgrDI-PacI-AbsI linker

<400>  156
cgtcgacgtt aattaacctc gagg                                              24


<210>  157
<211>  2007
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTwist-Kan-MC vector

<400>  157
cgcgcgcgtt aattaacctc gaggcctagg cgtcgacgtt aattaaggcg cgccaggcta       60

ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg      120

tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca      180

atctccaaga cattaggtgg agttcagttc ggcgagcgga aatggcttac gaacggggcg      240

gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa      300

gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca      360

gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct      420

cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg      480

tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact      540

gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg      600

agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta      660

gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt      720

gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc      780

gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca      840

aaacgatctc aagaagatca tcttattaag tctgacgctc tattcaacaa agccgccgtc      900

catgggtagg gggcttcaaa tcgtcccccc atacgatata agttgttact agtgcttgga      960

ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt     1020

tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc     1080

cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa     1140

gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg     1200

cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt     1260

taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat     1320

aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat     1380

gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt     1440

ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc     1500

tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa     1560

ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag     1620

ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt     1680

acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc     1740

ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc     1800

attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattgat acctgaaaca     1860

aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt     1920

tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga     1980

ataaggaagt gccattccgc ctgacct                                         2007


<210>  158
<211>  2159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTwist-Kan-MC vector with MauBI-PacI-AbsI-AvrII-SgrDI-PacI-AscI 
       polylinker

<400>  158
cgcgcgcgtt aattaacctc gaggcctagg cgtcgacgtt aattaaggcg cgccaggcta       60

ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg      120

tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca      180

atctccaaga cattaggtgg agttcagttc ggcgagcgga aatggcttac gaacggggcg      240

gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa      300

gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca      360

gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct      420

cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg      480

tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact      540

gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg      600

agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta      660

gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt      720

gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc      780

gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca      840

aaacgatctc aagaagatca tcttattaag tctgacgctc tattcaacaa agccgccgtc      900

catgggtagg gggcttcaaa tcgtccgctc tgccagtgtt acaaccaatt aacaaattct      960

gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca     1020

ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc     1080

cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa     1140

cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg     1200

actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc     1260

cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat     1320

tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc     1380

gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga     1440

tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca     1500

tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag     1560

tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga     1620

aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg     1680

acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc     1740

ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt     1800

atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat     1860

cagagatttt gagacacaac gtggctttcc cccgccgctc tagaactagt ggatccaaat     1920

aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcgcatta     1980

tacgagacgt ccaggttggg atacctgaaa caaaacccat cgtacggcca aggaagtctc     2040

caataactgt gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac     2100

ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct      2159


<210>  159
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (163)..(822)
<223>  cat gene from pACYC184

<400>  159
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aa atg gag aaa aaa         174
                                               Met Glu Lys Lys            
                                               1                          

atc act gga tat acc acc gtt gat ata tcc caa tgg cat cgt aaa gaa        222
Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp His Arg Lys Glu           
5                   10                  15                  20            

cat ttt gag gca ttt cag tca gtt gct caa tgt acc tat aac cag acc        270
His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr Tyr Asn Gln Thr           
                25                  30                  35                

gtt cag ctg gat att acg gcc ttt tta aag acc gta aag aaa aat aag        318
Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val Lys Lys Asn Lys           
            40                  45                  50                    

cac aag ttt tat ccg gcc ttt att cac att ctt gcc cgc ctg atg aat        366
His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala Arg Leu Met Asn           
        55                  60                  65                        

gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt gag ctg gtg ata        414
Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly Glu Leu Val Ile           
    70                  75                  80                            

tgg gat agt gtt cac cct tgt tac acc gtt ttc cat gag caa act gaa        462
Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His Glu Gln Thr Glu           
85                  90                  95                  100           

acg ttt tca tcg ctc tgg agt gaa tac cac gac gat ttc cgg cag ttt        510
Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp Phe Arg Gln Phe           
                105                 110                 115               

cta cac ata tat tcg caa gat gtg gcg tgt tac ggt gaa aac ctg gcc        558
Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly Glu Asn Leu Ala           
            120                 125                 130                   

tat ttc cct aaa ggg ttt att gag aat atg ttt ttc gtc tca gcc aat        606
Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe Val Ser Ala Asn           
        135                 140                 145                       

ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg gcc aat atg gac        654
Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val Ala Asn Met Asp           
    150                 155                 160                           

aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat tat acg caa ggc        702
Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr Tyr Thr Gln Gly           
165                 170                 175                 180           

gac aag gtg ctg atg ccg ctg gcg att cag gtt cat cat gcc gtc tgt        750
Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His His Ala Val Cys           
                185                 190                 195               

gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta caa cag tac tgc        798
Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu Gln Gln Tyr Cys           
            200                 205                 210                   

gat gag tgg cag ggc ggg gcg taa tttttttaag gcagttattg gtgcccgggc       852
Asp Glu Trp Gln Gly Gly Ala                                               
        215                                                               

cgtcgacgtt aattaaggcg cgcc                                             876


<210>  160
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  160

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr Cys Asp Glu Trp Gln Gly Gly Ala 
    210                 215                 


<210>  161
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (163)..(801)
<223>  cat gene from pACYC184 with one TAA stop codon

<400>  161
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aa atg gag aaa aaa         174
                                               Met Glu Lys Lys            
                                               1                          

atc act gga tat acc acc gtt gat ata tcc caa tgg cat cgt aaa gaa        222
Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp His Arg Lys Glu           
5                   10                  15                  20            

cat ttt gag gca ttt cag tca gtt gct caa tgt acc tat aac cag acc        270
His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr Tyr Asn Gln Thr           
                25                  30                  35                

gtt cag ctg gat att acg gcc ttt tta aag acc gta aag aaa aat aag        318
Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val Lys Lys Asn Lys           
            40                  45                  50                    

cac aag ttt tat ccg gcc ttt att cac att ctt gcc cgc ctg atg aat        366
His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala Arg Leu Met Asn           
        55                  60                  65                        

gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt gag ctg gtg ata        414
Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly Glu Leu Val Ile           
    70                  75                  80                            

tgg gat agt gtt cac cct tgt tac acc gtt ttc cat gag caa act gaa        462
Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His Glu Gln Thr Glu           
85                  90                  95                  100           

acg ttt tca tcg ctc tgg agt gaa tac cac gac gat ttc cgg cag ttt        510
Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp Phe Arg Gln Phe           
                105                 110                 115               

cta cac ata tat tcg caa gat gtg gcg tgt tac ggt gaa aac ctg gcc        558
Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly Glu Asn Leu Ala           
            120                 125                 130                   

tat ttc cct aaa ggg ttt att gag aat atg ttt ttc gtc tca gcc aat        606
Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe Val Ser Ala Asn           
        135                 140                 145                       

ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg gcc aat atg gac        654
Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val Ala Asn Met Asp           
    150                 155                 160                           

aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat tat acg caa ggc        702
Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr Tyr Thr Gln Gly           
165                 170                 175                 180           

gac aag gtg ctg atg ccg ctg gcg att cag gtt cat cat gcc gtc tgt        750
Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His His Ala Val Cys           
                185                 190                 195               

gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta caa cag tac tgc        798
Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu Gln Gln Tyr Cys           
            200                 205                 210                   

taa gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgcccggg             851

ccgtcgacgt taattaaggc gcgcc                                            876


<210>  162
<211>  212
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  162

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr Cys 
    210         


<210>  163
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (163)..(801)
<223>  cat gene from pACYC184 with two TAA stop codons

<400>  163
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aa atg gag aaa aaa         174
                                               Met Glu Lys Lys            
                                               1                          

atc act gga tat acc acc gtt gat ata tcc caa tgg cat cgt aaa gaa        222
Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp His Arg Lys Glu           
5                   10                  15                  20            

cat ttt gag gca ttt cag tca gtt gct caa tgt acc tat aac cag acc        270
His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr Tyr Asn Gln Thr           
                25                  30                  35                

gtt cag ctg gat att acg gcc ttt tta aag acc gta aag aaa aat aag        318
Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val Lys Lys Asn Lys           
            40                  45                  50                    

cac aag ttt tat ccg gcc ttt att cac att ctt gcc cgc ctg atg aat        366
His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala Arg Leu Met Asn           
        55                  60                  65                        

gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt gag ctg gtg ata        414
Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly Glu Leu Val Ile           
    70                  75                  80                            

tgg gat agt gtt cac cct tgt tac acc gtt ttc cat gag caa act gaa        462
Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His Glu Gln Thr Glu           
85                  90                  95                  100           

acg ttt tca tcg ctc tgg agt gaa tac cac gac gat ttc cgg cag ttt        510
Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp Phe Arg Gln Phe           
                105                 110                 115               

cta cac ata tat tcg caa gat gtg gcg tgt tac ggt gaa aac ctg gcc        558
Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly Glu Asn Leu Ala           
            120                 125                 130                   

tat ttc cct aaa ggg ttt att gag aat atg ttt ttc gtc tca gcc aat        606
Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe Val Ser Ala Asn           
        135                 140                 145                       

ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg gcc aat atg gac        654
Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val Ala Asn Met Asp           
    150                 155                 160                           

aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat tat acg caa ggc        702
Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr Tyr Thr Gln Gly           
165                 170                 175                 180           

gac aag gtg ctg atg ccg ctg gcg att cag gtt cat cat gcc gtc tgt        750
Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His His Ala Val Cys           
                185                 190                 195               

gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta caa cag tac taa        798
Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu Gln Gln Tyr               
            200                 205                 210                   

taa gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgcccggg             851

ccgtcgacgt taattaaggc gcgcc                                            876


<210>  164
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  164

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr 
    210     


<210>  165
<211>  889
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (163)..(801)
<223>  cat gene from pACYC184 and two TAA stop codons followed by 
       mini-attTn7 target site

<400>  165
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aa atg gag aaa aaa         174
                                               Met Glu Lys Lys            
                                               1                          

atc act gga tat acc acc gtt gat ata tcc caa tgg cat cgt aaa gaa        222
Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp His Arg Lys Glu           
5                   10                  15                  20            

cat ttt gag gca ttt cag tca gtt gct caa tgt acc tat aac cag acc        270
His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr Tyr Asn Gln Thr           
                25                  30                  35                

gtt cag ctg gat att acg gcc ttt tta aag acc gta aag aaa aat aag        318
Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val Lys Lys Asn Lys           
            40                  45                  50                    

cac aag ttt tat ccg gcc ttt att cac att ctt gcc cgc ctg atg aat        366
His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala Arg Leu Met Asn           
        55                  60                  65                        

gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt gag ctg gtg ata        414
Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly Glu Leu Val Ile           
    70                  75                  80                            

tgg gat agt gtt cac cct tgt tac acc gtt ttc cat gag caa act gaa        462
Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His Glu Gln Thr Glu           
85                  90                  95                  100           

acg ttt tca tcg ctc tgg agt gaa tac cac gac gat ttc cgg cag ttt        510
Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp Phe Arg Gln Phe           
                105                 110                 115               

cta cac ata tat tcg caa gat gtg gcg tgt tac ggt gaa aac ctg gcc        558
Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly Glu Asn Leu Ala           
            120                 125                 130                   

tat ttc cct aaa ggg ttt att gag aat atg ttt ttc gtc tca gcc aat        606
Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe Val Ser Ala Asn           
        135                 140                 145                       

ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg gcc aat atg gac        654
Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val Ala Asn Met Asp           
    150                 155                 160                           

aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat tat acg caa ggc        702
Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr Tyr Thr Gln Gly           
165                 170                 175                 180           

gac aag gtg ctg atg ccg ctg gcg att cag gtt cat cat gcc gtc tgt        750
Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His His Ala Val Cys           
                185                 190                 195               

gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta caa cag tac taa        798
Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu Gln Gln Tyr               
            200                 205                 210                   

taa agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg             851

ctgggtgccc gggccgtcga cgttaattaa ggcgcgcc                              889


<210>  166
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  166

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr 
    210     


<210>  167
<211>  896
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (163)..(813)
<223>  gene fusion comprising cat gene from pACYC194 fused to reading 
       frame 1 from end of Tn7L

<400>  167
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aa atg gag aaa aaa         174
                                               Met Glu Lys Lys            
                                               1                          

atc act gga tat acc acc gtt gat ata tcc caa tgg cat cgt aaa gaa        222
Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp His Arg Lys Glu           
5                   10                  15                  20            

cat ttt gag gca ttt cag tca gtt gct caa tgt acc tat aac cag acc        270
His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr Tyr Asn Gln Thr           
                25                  30                  35                

gtt cag ctg gat att acg gcc ttt tta aag acc gta aag aaa aat aag        318
Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val Lys Lys Asn Lys           
            40                  45                  50                    

cac aag ttt tat ccg gcc ttt att cac att ctt gcc cgc ctg atg aat        366
His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala Arg Leu Met Asn           
        55                  60                  65                        

gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt gag ctg gtg ata        414
Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly Glu Leu Val Ile           
    70                  75                  80                            

tgg gat agt gtt cac cct tgt tac acc gtt ttc cat gag caa act gaa        462
Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His Glu Gln Thr Glu           
85                  90                  95                  100           

acg ttt tca tcg ctc tgg agt gaa tac cac gac gat ttc cgg cag ttt        510
Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp Phe Arg Gln Phe           
                105                 110                 115               

cta cac ata tat tcg caa gat gtg gcg tgt tac ggt gaa aac ctg gcc        558
Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly Glu Asn Leu Ala           
            120                 125                 130                   

tat ttc cct aaa ggg ttt att gag aat atg ttt ttc gtc tca gcc aat        606
Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe Val Ser Ala Asn           
        135                 140                 145                       

ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg gcc aat atg gac        654
Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val Ala Asn Met Asp           
    150                 155                 160                           

aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat tat acg caa ggc        702
Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr Tyr Thr Gln Gly           
165                 170                 175                 180           

gac aag gtg ctg atg ccg ctg gcg att cag gtt cat cat gcc gtc tgt        750
Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His His Ala Val Cys           
                185                 190                 195               

gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta caa cag tac tgt        798
Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu Gln Gln Tyr Cys           
            200                 205                 210                   

ggg cgg aca aaa tag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt        853
Gly Arg Thr Lys                                                           
        215                                                               

atttaggggg tgcccgggcc gtcgacgtta attaaggcgc gcc                        896


<210>  168
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  168

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr Cys Gly Arg Thr Lys 
    210                 215     


<210>  169
<211>  897
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (163)..(849)
<223>  gene fusion comprising cat gene from pACYC194 fused to reading 
       frame 2 from end of Tn7L

<400>  169
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aa atg gag aaa aaa         174
                                               Met Glu Lys Lys            
                                               1                          

atc act gga tat acc acc gtt gat ata tcc caa tgg cat cgt aaa gaa        222
Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp His Arg Lys Glu           
5                   10                  15                  20            

cat ttt gag gca ttt cag tca gtt gct caa tgt acc tat aac cag acc        270
His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr Tyr Asn Gln Thr           
                25                  30                  35                

gtt cag ctg gat att acg gcc ttt tta aag acc gta aag aaa aat aag        318
Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val Lys Lys Asn Lys           
            40                  45                  50                    

cac aag ttt tat ccg gcc ttt att cac att ctt gcc cgc ctg atg aat        366
His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala Arg Leu Met Asn           
        55                  60                  65                        

gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt gag ctg gtg ata        414
Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly Glu Leu Val Ile           
    70                  75                  80                            

tgg gat agt gtt cac cct tgt tac acc gtt ttc cat gag caa act gaa        462
Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His Glu Gln Thr Glu           
85                  90                  95                  100           

acg ttt tca tcg ctc tgg agt gaa tac cac gac gat ttc cgg cag ttt        510
Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp Phe Arg Gln Phe           
                105                 110                 115               

cta cac ata tat tcg caa gat gtg gcg tgt tac ggt gaa aac ctg gcc        558
Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly Glu Asn Leu Ala           
            120                 125                 130                   

tat ttc cct aaa ggg ttt att gag aat atg ttt ttc gtc tca gcc aat        606
Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe Val Ser Ala Asn           
        135                 140                 145                       

ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg gcc aat atg gac        654
Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val Ala Asn Met Asp           
    150                 155                 160                           

aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat tat acg caa ggc        702
Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr Tyr Thr Gln Gly           
165                 170                 175                 180           

gac aag gtg ctg atg ccg ctg gcg att cag gtt cat cat gcc gtc tgt        750
Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His His Ala Val Cys           
                185                 190                 195               

gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta caa cag tac ttg        798
Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu Gln Gln Tyr Leu           
            200                 205                 210                   

tgg gcg gac aaa ata gtt ggg aac tgg gag ggg tgg aaa tgg agt ttt        846
Trp Ala Asp Lys Ile Val Gly Asn Trp Glu Gly Trp Lys Trp Ser Phe           
        215                 220                 225                       

taa ggattattta gggggtgccc gggccgtcga cgttaattaa ggcgcgcc               897


<210>  170
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  170

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr Leu Trp Ala Asp Lys Ile Val Gly Asn Trp Glu Gly Trp 
    210                 215                 220                 


Lys Trp Ser Phe 
225             


<210>  171
<211>  898
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (163)..(822)
<223>  gene fusion comprising cat gene from pACYC194 fused to reading 
       frame 3 from end of Tn7L

<400>  171
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aa atg gag aaa aaa         174
                                               Met Glu Lys Lys            
                                               1                          

atc act gga tat acc acc gtt gat ata tcc caa tgg cat cgt aaa gaa        222
Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp His Arg Lys Glu           
5                   10                  15                  20            

cat ttt gag gca ttt cag tca gtt gct caa tgt acc tat aac cag acc        270
His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr Tyr Asn Gln Thr           
                25                  30                  35                

gtt cag ctg gat att acg gcc ttt tta aag acc gta aag aaa aat aag        318
Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val Lys Lys Asn Lys           
            40                  45                  50                    

cac aag ttt tat ccg gcc ttt att cac att ctt gcc cgc ctg atg aat        366
His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala Arg Leu Met Asn           
        55                  60                  65                        

gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt gag ctg gtg ata        414
Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly Glu Leu Val Ile           
    70                  75                  80                            

tgg gat agt gtt cac cct tgt tac acc gtt ttc cat gag caa act gaa        462
Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His Glu Gln Thr Glu           
85                  90                  95                  100           

acg ttt tca tcg ctc tgg agt gaa tac cac gac gat ttc cgg cag ttt        510
Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp Phe Arg Gln Phe           
                105                 110                 115               

cta cac ata tat tcg caa gat gtg gcg tgt tac ggt gaa aac ctg gcc        558
Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly Glu Asn Leu Ala           
            120                 125                 130                   

tat ttc cct aaa ggg ttt att gag aat atg ttt ttc gtc tca gcc aat        606
Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe Val Ser Ala Asn           
        135                 140                 145                       

ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg gcc aat atg gac        654
Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val Ala Asn Met Asp           
    150                 155                 160                           

aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat tat acg caa ggc        702
Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr Tyr Thr Gln Gly           
165                 170                 175                 180           

gac aag gtg ctg atg ccg ctg gcg att cag gtt cat cat gcc gtc tgt        750
Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His His Ala Val Cys           
                185                 190                 195               

gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta caa cag tac tat        798
Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu Gln Gln Tyr Tyr           
            200                 205                 210                   

gtg ggc gga caa aat agt tgg gaa ctgggagggg tggaaatgga gtttttaagg       852
Val Gly Gly Gln Asn Ser Trp Glu                                           
        215                 220                                           

attatttagg gggtgcccgg gccgtcgacg ttaattaagg cgcgcc                     898


<210>  172
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  172

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr Tyr Val Gly Gly Gln Asn Ser Trp Glu 
    210                 215                 220 


<210>  173
<211>  1953
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTwist-Chlor-MC cloining vector

<400>  173
aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca       60

tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat      120

ccgacaatct ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac      180

ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc      240

ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc      300

aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg      360

ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg      420

gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc      480

tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc      540

gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt      600

gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt      660

tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga      720

accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc      780

agaccaaaac gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc      840

gccgtccatg ggtagggggc ttcaaatcgt ccccccatac gatataagtt gttactagtg      900

cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga      960

tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa     1020

ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac     1080

atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc     1140

gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc     1200

cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt     1260

ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga     1320

atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt     1380

ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc     1440

accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg     1500

aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat     1560

atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg     1620

ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat     1680

tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct     1740

tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attgatacct     1800

gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc     1860

cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca     1920

tctggaataa ggaagtgcca ttccgcctga cct                                  1953


<210>  174
<211>  2007
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTwist+Chlor+MC vectior with 
       MauBI-PacI-AbsI-AvrII-SgrDI-PacI-AscI polylinker

<400>  174
cgcgcgcgtt aattaacctc gaggcctagg cgtcgacgtt aattaaggcg cgccaggcta       60

ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg      120

tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca      180

atctccaaga cattaggtgg agttcagttc ggcgagcgga aatggcttac gaacggggcg      240

gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa      300

gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca      360

gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct      420

cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg      480

tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact      540

gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg      600

agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta      660

gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt      720

gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc      780

gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca      840

aaacgatctc aagaagatca tcttattaag tctgacgctc tattcaacaa agccgccgtc      900

catgggtagg gggcttcaaa tcgtcccccc atacgatata agttgttact agtgcttgga      960

ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt     1020

tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc     1080

cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa     1140

gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg     1200

cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt     1260

taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat     1320

aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat     1380

gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt     1440

ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc     1500

tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa     1560

ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag     1620

ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt     1680

acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc     1740

ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc     1800

attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattgat acctgaaaca     1860

aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt     1920

tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga     1980

ataaggaagt gccattccgc ctgacct                                         2007


<210>  175
<211>  1028
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(971)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode CGRTK and one stop codon

<400>  175
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gaa ttc ttc tgt ggg cgg aca aaa tag taa ctagttaatt ggttgtaaca      991
Asp Glu Phe Phe Cys Gly Arg Thr Lys                                       
        270                 275                                           

ctggcagagc aggcgtcgac gttaattaag gcgcgcc                              1028


<210>  176
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  176

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Cys 
            260                 265                 270         


Gly Arg Thr Lys 
        275     


<210>  177
<211>  1040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(983)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode PSFNAVVYHS and one stop codon

<400>  177
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gaa ttc ttc cca agc ttt aat gcg gta gtt tat cac agt taa            983
Asp Glu Phe Phe Pro Ser Phe Asn Ala Val Val Tyr His Ser                   
        270                 275                 280                       

ctagttaatt ggttgtaaca ctggcagagc aggcgtcgac gttaattaag gcgcgcc        1040


<210>  178
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  178

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Pro 
            260                 265                 270         


Ser Phe Asn Ala Val Val Tyr His Ser 
        275                 280     


<210>  179
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(959)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode PS and one stop codon

<400>  179
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gaa ttc ttc cca agc taa ctagttaatt ggttgtaaca ctggcagagc           989
Asp Glu Phe Phe Pro Ser                                                   
        270                                                               

aggcgtcgac gttaattaag gcgcgcc                                         1016


<210>  180
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  180

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Pro 
            260                 265                 270         


Ser 
    


<210>  181
<211>  1074
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(968)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode CGRTK and one stop codon followed by partial Tn7L

<400>  181
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gaa ttc ttc tgt ggg cgg aca aaa tag ttgggaactg ggaggggtgg          988
Asp Glu Phe Phe Cys Gly Arg Thr Lys                                       
        270                 275                                           

aaatggagtt tttaaggatt atttagggac tagttaattg gttgtaacac tggcagagca     1048

ggcgtcgacg ttaattaagg cgcgcc                                          1074


<210>  182
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  182

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Cys 
            260                 265                 270         


Gly Arg Thr Lys 
        275     


<210>  183
<211>  1075
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(1004)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode LWADKIVGNWEGWKWSF and one stop codon followed by 
       partial Tn7L in reading frame 2

<400>  183
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gaa ttc ttc ctg tgg gcg gac aaa ata gtt ggg aac tgg gag ggg        986
Asp Glu Phe Phe Leu Trp Ala Asp Lys Ile Val Gly Asn Trp Glu Gly           
        270                 275                 280                       

tgg aaa tgg agt ttt taa ggattattta gggactagtt aattggttgt              1034
Trp Lys Trp Ser Phe                                                       
    285                                                                   

aacactggca gagcaggcgt cgacgttaat taaggcgcgc c                         1075


<210>  184
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  184

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Leu 
            260                 265                 270         


Trp Ala Asp Lys Ile Val Gly Asn Trp Glu Gly Trp Lys Trp Ser Phe 
        275                 280                 285             


<210>  185
<211>  1076
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(1007)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode PVGSQNSWELGGVEMEFLRII and one stop codon in reading 
       frame 3

<400>  185
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gaa ttc ttc cct gtg ggc gga caa aat agt tgg gaa ctg gga ggg        986
Asp Glu Phe Phe Pro Val Gly Gly Gln Asn Ser Trp Glu Leu Gly Gly           
        270                 275                 280                       

gtg gaa atg gag ttt tta agg attatttagg gactagttaa ttggttgtaa          1037
Val Glu Met Glu Phe Leu Arg                                               
    285                 290                                               

cactggcaga gcaggcgtcg acgttaatta aggcgcgcc                            1076


<210>  186
<211>  290
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  186

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Pro 
            260                 265                 270         


Val Gly Gly Gln Asn Ser Trp Glu Leu Gly Gly Val Glu Met Glu Phe 
        275                 280                 285             


Leu Arg 
    290 


<210>  187
<211>  1069
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(959)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode PS and one stop codon and overlapping mini-attTn7 
       site

<400>  187
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gaa ttc ttc cca agc taa gcatccattt attactcaac cgtaaccgat           989
Asp Glu Phe Phe Pro Ser                                                   
        270                                                               

tttgccaggt tacgcggctg taactagtta attggttgta acactggcag agcaggcgtc     1049

gacgttaatt aaggcgcgcc                                                 1069


<210>  188
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  188

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Pro 
            260                 265                 270         


Ser 
    


<210>  189
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(959)
<223>  gene fusion comprising kanamycin gene from pACYC177 extended to 
       also encode PS and one stop codon

<400>  189
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gag ttt ttc cca agc taa ttggttaatt ggttgtaaca ctggcagagc           989
Asp Glu Phe Phe Pro Ser                                                   
        270                                                               

aggcgtcgac gttaattaag gcgcgcc                                         1016


<210>  190
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  190

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe Pro 
            260                 265                 270         


Ser 
    


<210>  191
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (138)..(953)
<223>  Unaltered kanamycn gene from pACYC177 and one TAA stop codon

<400>  191
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgtt atg agc cat att caa cgg gaa acg tct tgc tcg         170
                   Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser            
                   1               5                   10                 

agg ccg cga tta aat tcc aac atg gat gct gat tta tat ggg tat aaa        218
Arg Pro Arg Leu Asn Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys           
            15                  20                  25                    

tgg gct cgc gat aat gtc ggg caa tca ggt gcg aca atc tat cga ttg        266
Trp Ala Arg Asp Asn Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu           
        30                  35                  40                        

tat ggg aag ccc gat gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt        314
Tyr Gly Lys Pro Asp Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly           
    45                  50                  55                            

agc gtt gcc aat gat gtt aca gat gag atg gtc aga cta aac tgg ctg        362
Ser Val Ala Asn Asp Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu           
60                  65                  70                  75            

acg gaa ttt atg cct ctt ccg acc atc aag cat ttt atc cgt act cct        410
Thr Glu Phe Met Pro Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro           
                80                  85                  90                

gat gat gca tgg tta ctc acc act gcg atc ccc ggg aaa aca gca ttc        458
Asp Asp Ala Trp Leu Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe           
            95                  100                 105                   

cag gta tta gaa gaa tat cct gat tca ggt gaa aat att gtt gat gcg        506
Gln Val Leu Glu Glu Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala           
        110                 115                 120                       

ctg gca gtg ttc ctg cgc cgg ttg cat tcg att cct gtt tgt aat tgt        554
Leu Ala Val Phe Leu Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys           
    125                 130                 135                           

cct ttt aac agc gat cgc gta ttt cgt ctc gct cag gcg caa tca cga        602
Pro Phe Asn Ser Asp Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg           
140                 145                 150                 155           

atg aat aac ggt ttg gtt gat gcg agt gat ttt gat gac gag cgt aat        650
Met Asn Asn Gly Leu Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn           
                160                 165                 170               

ggc tgg cct gtt gaa caa gtc tgg aaa gaa atg cat aag ctt ttg cca        698
Gly Trp Pro Val Glu Gln Val Trp Lys Glu Met His Lys Leu Leu Pro           
            175                 180                 185                   

ttc tca ccg gat tca gtc gtc act cat ggt gat ttc tca ctt gat aac        746
Phe Ser Pro Asp Ser Val Val Thr His Gly Asp Phe Ser Leu Asp Asn           
        190                 195                 200                       

ctt att ttt gac gag ggg aaa tta ata ggt tgt att gat gtt gga cga        794
Leu Ile Phe Asp Glu Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg           
    205                 210                 215                           

gtc gga atc gca gac cga tac cag gat ctt gcc atc cta tgg aac tgc        842
Val Gly Ile Ala Asp Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys           
220                 225                 230                 235           

ctc ggt gag ttt tct cct tca tta cag aaa cgg ctt ttt caa aaa tat        890
Leu Gly Glu Phe Ser Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr           
                240                 245                 250               

ggt att gat aat cct gat atg aat aaa ttg cag ttt cat ttg atg ctc        938
Gly Ile Asp Asn Pro Asp Met Asn Lys Leu Gln Phe His Leu Met Leu           
            255                 260                 265                   

gat gag ttt ttc taa tcagaattgg ttaattggtt gtaacactgg cagagcaggc        993
Asp Glu Phe Phe                                                           
        270                                                               

gtcgacgtta attaaggcgc gcc                                             1016


<210>  192
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  192

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
            260                 265                 270     


<210>  193
<211>  837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (262)..(804)
<223>  lacZalpha gene comprising mini-attTn7 target site

<400>  193
cgcgcgcgtt aattaacctc gaggcctcct gcaggaatta agtcttcgaa ccaatacgca       60

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg      120

actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac      180

cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac      240

aatttcacac aggaaacagc t atg acc atg att acg cca agc tcg gaa tta        291
                        Met Thr Met Ile Thr Pro Ser Ser Glu Leu           
                        1               5                   10            

acc ctc act aaa ggg aac aaa agc tgg agc tcc acc gcg gtg gcg gcc        339
Thr Leu Thr Lys Gly Asn Lys Ser Trp Ser Ser Thr Ala Val Ala Ala           
                15                  20                  25                

gct cta gaa cta gtg gat ccc ccg ggc tgc agg aat tca cat aac agg        387
Ala Leu Glu Leu Val Asp Pro Pro Gly Cys Arg Asn Ser His Asn Arg           
            30                  35                  40                    

aag aaa aat gcc ccg ctt acg cag ggc atc cat tta tta ctc aac cgt        435
Lys Lys Asn Ala Pro Leu Thr Gln Gly Ile His Leu Leu Leu Asn Arg           
        45                  50                  55                        

aac cga ttt tgc cag gtt acg cgg ctg tcg acc tcg agg ggg ggc ccg        483
Asn Arg Phe Cys Gln Val Thr Arg Leu Ser Thr Ser Arg Gly Gly Pro           
    60                  65                  70                            

gta ccc aat tcg ccc tat agt gag tcg tat tac gcg cgc tca ctg gcc        531
Val Pro Asn Ser Pro Tyr Ser Glu Ser Tyr Tyr Ala Arg Ser Leu Ala           
75                  80                  85                  90            

gtc gtt tta caa cgt cgt gac tgg gaa aac cct ggc gtt acc caa ctt        579
Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu           
                95                  100                 105               

aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt aat agc gaa        627
Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu           
            110                 115                 120                   

gag gcc cgc acc gat cgc cct tcc caa cag ttg cgc agc ctg aat ggc        675
Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly           
        125                 130                 135                       

gaa tgg gac gcg ccc tgt agc ggc gca tta agc gcg gcg ggt gtg gtg        723
Glu Trp Asp Ala Pro Cys Ser Gly Ala Leu Ser Ala Ala Gly Val Val           
    140                 145                 150                           

gtt acg cgc ttc gaa gac gct cga cag cga cac act tgc atc gga tgc        771
Val Thr Arg Phe Glu Asp Ala Arg Gln Arg His Thr Cys Ile Gly Cys           
155                 160                 165                 170           

agc ccg gtt aac gtg ccg gca cgg cct ggg taa ccaggtaggc gtcgacgtta      824
Ser Pro Val Asn Val Pro Ala Arg Pro Gly                                   
                175                 180                                   

attaaggcgc gcc                                                         837


<210>  194
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  194

Met Thr Met Ile Thr Pro Ser Ser Glu Leu Thr Leu Thr Lys Gly Asn 
1               5                   10                  15      


Lys Ser Trp Ser Ser Thr Ala Val Ala Ala Ala Leu Glu Leu Val Asp 
            20                  25                  30          


Pro Pro Gly Cys Arg Asn Ser His Asn Arg Lys Lys Asn Ala Pro Leu 
        35                  40                  45              


Thr Gln Gly Ile His Leu Leu Leu Asn Arg Asn Arg Phe Cys Gln Val 
    50                  55                  60                  


Thr Arg Leu Ser Thr Ser Arg Gly Gly Pro Val Pro Asn Ser Pro Tyr 
65                  70                  75                  80  


Ser Glu Ser Tyr Tyr Ala Arg Ser Leu Ala Val Val Leu Gln Arg Arg 
                85                  90                  95      


Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His 
            100                 105                 110         


Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg 
        115                 120                 125             


Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Asp Ala Pro Cys 
    130                 135                 140                 


Ser Gly Ala Leu Ser Ala Ala Gly Val Val Val Thr Arg Phe Glu Asp 
145                 150                 155                 160 


Ala Arg Gln Arg His Thr Cys Ile Gly Cys Ser Pro Val Asn Val Pro 
                165                 170                 175     


Ala Arg Pro Gly 
            180 


<210>  195
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  CDS
<222>  (262)..(654)
<223>  lacZalpha gene comprising micro-attTn7 target site

<400>  195
cgcgcgcgtt aattaacctc gaggcctcct gcaggaatta agtcttcgaa ccaatacgca       60

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg      120

actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac      180

cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac      240

aatttcacac aggaaacagc t atg acc atg att acg aat tca cat aac agg        291
                        Met Thr Met Ile Thr Asn Ser His Asn Arg           
                        1               5                   10            

aag aaa aat gcc ccg ctt acg cag ggc atc cat tta tta ctc aac cgt        339
Lys Lys Asn Ala Pro Leu Thr Gln Gly Ile His Leu Leu Leu Asn Arg           
                15                  20                  25                

aac cga ttt tgc cag gtt acg cgg ctg tcg act tca ctg gcc gtc gtt        387
Asn Arg Phe Cys Gln Val Thr Arg Leu Ser Thr Ser Leu Ala Val Val           
            30                  35                  40                    

tta caa cgt cgt gac tgg gaa aac cct ggc gtt acc caa ctt aat cgc        435
Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg           
        45                  50                  55                        

ctt gca gca cat ccc cct ttc gcc agc tgg cgt aat agc gaa gag gcc        483
Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala           
    60                  65                  70                            

cgc acc gat cgc cct tcc caa cag ttg cgc agc ctg aat ggc gaa tgg        531
Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp           
75                  80                  85                  90            

gac gcg ccc tgt agc ggc gca tta agc gcg gcg ggt gtg gtg gtt acg        579
Asp Ala Pro Cys Ser Gly Ala Leu Ser Ala Ala Gly Val Val Val Thr           
                95                  100                 105               

cgc ttc gaa gac gct cga cag cga cac act tgc atc gga tgc agc ccg        627
Arg Phe Glu Asp Ala Arg Gln Arg His Thr Cys Ile Gly Cys Ser Pro           
            110                 115                 120                   

gtt aac gtg ccg gca cgg cct ggg taa ccaggtaggc gtcgacgtta              674
Val Asn Val Pro Ala Arg Pro Gly                                           
        125                 130                                           

attaaggcgc gcc                                                         687


<210>  196
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  196

Met Thr Met Ile Thr Asn Ser His Asn Arg Lys Lys Asn Ala Pro Leu 
1               5                   10                  15      


Thr Gln Gly Ile His Leu Leu Leu Asn Arg Asn Arg Phe Cys Gln Val 
            20                  25                  30          


Thr Arg Leu Ser Thr Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp 
        35                  40                  45              


Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro 
    50                  55                  60                  


Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser 
65                  70                  75                  80  


Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Asp Ala Pro Cys Ser Gly 
                85                  90                  95      


Ala Leu Ser Ala Ala Gly Val Val Val Thr Arg Phe Glu Asp Ala Arg 
            100                 105                 110         


Gln Arg His Thr Cys Ile Gly Cys Ser Pro Val Asn Val Pro Ala Arg 
        115                 120                 125             


Pro Gly 
    130 


<210>  197
<211>  2221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTwist-Amp-HC cloning vector

<400>  197
aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca       60

tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat      120

ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa      180

agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg      240

cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga      300

ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg      360

tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg      420

gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc      480

gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg      540

gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca      600

ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt      660

ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag      720

ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg      780

gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc      840

ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc      900

agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt      960

ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa     1020

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     1080

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     1140

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     1200

gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca     1260

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     1320

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     1380

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     1440

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     1500

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     1560

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     1620

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     1680

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     1740

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     1800

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     1860

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     1920

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt     1980

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc     2040

acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc     2100

tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg     2160

acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc     2220

t                                                                     2221


<210>  198
<211>  2275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTwist+Amp+HC with MauBI-AbsI-AvrII-SgrDI-AscI polylinker

<400>  198
cgcgcgcgtt aattaacctc gaggcctagg cgtcgacgtt aattaaggcg cgccaggcta       60

ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg      120

tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca      180

atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca      240

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt      300

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc      360

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct      420

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg      480

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca      540

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact      600

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta      660

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta      720

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct      780

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt      840

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga      900

tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta      960

aatgggtagg gggcttcaaa tcgtcctcgt gataccaatt cggagcctgc ttttttgtac     1020

aaacttgttg ataatggcaa ttcaaggatc ttcacctaga tccttttaaa ttaaaaatga     1080

agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     1140

atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     1200

cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     1260

ataccgcgag agccacgctc accggctcca gatttatcag caataaacca gccagccgga     1320

agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     1380

tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     1440

gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     1500

caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     1560

ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     1620

gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     1680

tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     1740

tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     1800

cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     1860

cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     1920

gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     1980

atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     2040

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     2100

ccccgaaaag tgccagatac ctgaaacaaa acccatcgta cggccaagga agtctccaat     2160

aactgtgatc caccacaagc gccagggttt tcccagtcac gacgttgtaa aacgacggcc     2220

agtcatgcat aatccgcacg catctggaat aaggaagtgc cattccgcct gacct          2275


<210>  199
<211>  546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  misc_feature
<222>  (27)..(521)
<223>  new mini-Tn7 with Tn7L - 
       MauBI-PacI-AbsI-AvrII(PstI)SacII-SgrDI-PacI-AscI polylinker-Tn7R

<400>  199
acataacagg aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggcctaggc ctgcaggccg      240

cggcgtcgac gttaattaag gcgcgccagt tgttcggtaa attgtcacaa cgccgcgaat      300

atagtcttta ccatgccctt ggccacgccc ctctttaata cgacgggcaa tttgcacttc      360

agaaaatgaa gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa      420

ctggactgat ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta      480

gatctatttt gttcagttta agactttatt gtccgcccac acccgcttac gcagggcatc      540

cattta                                                                 546


<210>  200
<211>  986
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA


<220>
<221>  misc_feature
<222>  (27)..(961)
<223>  new mini-Tn7 with lacZalpha gene from pUC18 between Tn7L and Tn7R

<220>
<221>  CDS
<222>  (438)..(680)
<223>  lacZalpha gene ending with two TAA stop codons with internal MCS 
       region derived from pUC18 bwtween Tn7L and Tn7R in a new mini-Tn7
       sequence

<400>  200
acataacagg aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggcctccaa tacgcaaacc      240

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg      300

gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca      360

ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt      420

tcacacagga aacagct atg acc atg att acg aat tcg agc tcg gta ccc         470
                   Met Thr Met Ile Thr Asn Ser Ser Ser Val Pro            
                   1               5                   10                 

ggg gat cct cta gag tcg acc tgc agg cat gca agc ttg gca ctg gcc        518
Gly Asp Pro Leu Glu Ser Thr Cys Arg His Ala Ser Leu Ala Leu Ala           
            15                  20                  25                    

gtc gtt tta caa cgt cgt gac tgg gaa aac cct ggc gtt acc caa ctt        566
Val Val Leu Gln Arg Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu           
        30                  35                  40                        

aat cgc ctt gca gca cat ccc cct ttc gcc agc tgg cgt aat agc gaa        614
Asn Arg Leu Ala Ala His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu           
    45                  50                  55                            

gag gcc cgc acc gat cgc cct tcc caa cag ttg cgc agc ctg aat ggc        662
Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly           
60                  65                  70                  75            

gaa tgg cgc ctt taa taa aggcgtcgac gttaattaag gcgcgccagt               710
Glu Trp Arg Leu                                                           
                                                                          

tgttcggtaa attgtcacaa cgccgcgaat atagtcttta ccatgccctt ggccacgccc      770

ctctttaata cgacgggcaa tttgcacttc agaaaatgaa gagtttgctt tagccataac      830

aaaagtccag tatgcttttt cacagcataa ctggactgat ttcagtttac aactattctg      890

tctagtttaa gactttattg tcatagttta gatctatttt gttcagttta agactttatt      950

gtccgcccac acccgcttac gcagggcatc cattta                                986


<210>  201
<211>  79
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  201

Met Thr Met Ile Thr Asn Ser Ser Ser Val Pro Gly Asp Pro Leu Glu 
1               5                   10                  15      


Ser Thr Cys Arg His Ala Ser Leu Ala Leu Ala Val Val Leu Gln Arg 
            20                  25                  30          


Arg Asp Trp Glu Asn Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala 
        35                  40                  45              


His Pro Pro Phe Ala Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp 
    50                  55                  60                  


Arg Pro Ser Gln Gln Leu Arg Ser Leu Asn Gly Glu Trp Arg Leu 
65                  70                  75                  


<210>  202
<211>  1028
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  202
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc tgtgggcgga      960

caaaatagta actagttaat tggttgtaac actggcagag caggcgtcga cgttaattaa     1020

ggcgcgcc                                                              1028


<210>  203
<211>  1028
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  203
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc tgtgggcgga      960

caaaatagta actagttaat tggttgtaac actggcagag caggcgtcga cgttaattaa     1020

ggcgcgcc                                                              1028


<210>  204
<211>  1040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  204
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc ccaagcttta      960

atgcggtagt ttatcacagt taactagtta attggttgta acactggcag agcaggcgtc     1020

gacgttaatt aaggcgcgcc                                                 1040


<210>  205
<211>  1069
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  205
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc ccaagctaag      960

catccattta ttactcaacc gtaaccgatt ttgccaggtt acgcggctgt aactagttaa     1020

ttggttgtaa cactggcaga gcaggcgtcg acgttaatta aggcgcgcc                 1069


<210>  206
<211>  1074
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  206
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc tgtgggcgga      960

caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat ttagggacta     1020

gttaattggt tgtaacactg gcagagcagg cgtcgacgtt aattaaggcg cgcc           1074


<210>  207
<211>  1075
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  207
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc ctgtgggcgg      960

acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggact     1020

agttaattgg ttgtaacact ggcagagcag gcgtcgacgt taattaaggc gcgcc          1075


<210>  208
<211>  1076
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  208
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc cctgtgggcg      960

gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt atttagggac     1020

tagttaattg gttgtaacac tggcagagca ggcgtcgacg ttaattaagg cgcgcc         1076


<210>  209
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  209
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc ccaagctaat      960

tggttaattg gttgtaacac tggcagagca ggcgtcgacg ttaattaagg cgcgcc         1016


<210>  210
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  210
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat      960

tggttaattg gttgtaacac tggcagagca ggcgtcgacg ttaattaagg cgcgcc         1016


<210>  211
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  211
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact      180

ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag      240

tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag      300

accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg      360

atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat      420

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg      480

agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt      540

tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca      600

gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc      660

ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg      720

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat      780

gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat      840

tggtgcccgg gccgtcgacg ttaattaagg cgcgcc                                876


<210>  212
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  212
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact      180

ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag      240

tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag      300

accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg      360

atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat      420

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg      480

agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt      540

tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca      600

gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc      660

ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg      720

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat      780

gaattacaac agtactgcta agagtggcag ggcggggcgt aattttttta aggcagttat      840

tggtgcccgg gccgtcgacg ttaattaagg cgcgcc                                876


<210>  213
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  213
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact      180

ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag      240

tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag      300

accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg      360

atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat      420

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg      480

agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt      540

tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca      600

gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc      660

ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg      720

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat      780

gaattacaac agtactaata agagtggcag ggcggggcgt aattttttta aggcagttat      840

tggtgcccgg gccgtcgacg ttaattaagg cgcgcc                                876


<210>  214
<211>  889
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  214
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact      180

ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag      240

tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag      300

accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg      360

atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat      420

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg      480

agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt      540

tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca      600

gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc      660

ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg      720

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat      780

gaattacaac agtactaata aagggcatcc atttattact caaccgtaac cgattttgcc      840

aggttacgcg gctgggtgcc cgggccgtcg acgttaatta aggcgcgcc                  889


<210>  215
<211>  896
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  215
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact      180

ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag      240

tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag      300

accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg      360

atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat      420

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg      480

agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt      540

tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca      600

gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc      660

ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg      720

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat      780

gaattacaac agtactgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga      840

gtttttaagg attatttagg gggtgcccgg gccgtcgacg ttaattaagg cgcgcc          896


<210>  216
<211>  897
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  216
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact      180

ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag      240

tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag      300

accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg      360

atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat      420

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg      480

agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt      540

tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca      600

gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc      660

ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg      720

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat      780

gaattacaac agtacttgtg ggcggacaaa atagttggga actgggaggg gtggaaatgg      840

agtttttaag gattatttag ggggtgcccg ggccgtcgac gttaattaag gcgcgcc         897


<210>  217
<211>  898
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  217
cgcgcgcgtt aattaacctc gaggcctccc tgggccaact tttggcgaaa atgagacgtt       60

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta      120

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact      180

ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag      240

tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag      300

accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg      360

atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat      420

agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg      480

agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt      540

tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca      600

gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc      660

ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg      720

ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat      780

gaattacaac agtactatgt gggcggacaa aatagttggg aactgggagg ggtggaaatg      840

gagtttttaa ggattattta gggggtgccc gggccgtcga cgttaattaa ggcgcgcc        898


<210>  218
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  218
cgcgcgcgtt aattaacctc gaggcctcct gcaggaatta agtcttcgaa ccaatacgca       60

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg      120

actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac      180

cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac      240

aatttcacac aggaaacagc tatgaccatg attacgaatt cacataacag gaagaaaaat      300

gccccgctta cgcagggcat ccatttatta ctcaaccgta accgattttg ccaggttacg      360

cggctgtcga cttcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt      420

acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag      480

gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc      540

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcttcgaaga cgctcgacag      600

cgacacactt gcatcggatg cagcccggtt aacgtgccgg cacggcctgg gtaaccaggt      660

aggcgtcgac gttaattaag gcgcgcc                                          687


<210>  219
<211>  837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  219
cgcgcgcgtt aattaacctc gaggcctcct gcaggaatta agtcttcgaa ccaatacgca       60

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg      120

actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac      180

cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac      240

aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt aaccctcact      300

aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatccc      360

ccgggctgca ggaattcaca taacaggaag aaaaatgccc cgcttacgca gggcatccat      420

ttattactca accgtaaccg attttgccag gttacgcggc tgtcgacctc gagggggggc      480

ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta      540

caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc      600

cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg      660

cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg      720

gtggttacgc gcttcgaaga cgctcgacag cgacacactt gcatcggatg cagcccggtt      780

aacgtgccgg cacggcctgg gtaaccaggt aggcgtcgac gttaattaag gcgcgcc         837


<210>  220
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  220
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgaattcttc taatcagaat      960

tggttaattg gttgtaacac tggcagagca ggcgtcgacg ttaattaagg cgcgcc         1016


<210>  221
<211>  1025
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  221
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagttttgt gggcggacaa      960

aatagtaatt ggttaattgg ttgtaacact ggcagagcag gcgtcgacgt taattaaggc     1020

gcgcc                                                                 1025


<210>  222
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  222
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagctgttc taatcagaat      960

tggttaattg gttgtaacac tggcagagca ggcgtcgacg ttaattaagg cgcgcc         1016


<210>  223
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  223
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagctgttc ccaagctaat      960

tggttaattg gttgtaacac tggcagagca ggcgtcgacg ttaattaagg cgcgcc         1016


<210>  224
<211>  1021
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  224
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagctgttt aattaacaat      960

aaagttggtt aattggttgt aacactggca gagcaggcgt cgacgttaat taaggcgcgc     1020

c                                                                     1021


<210>  225
<211>  1022
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  225
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagctgttt taattaacaa      960

taaagttggt taattggttg taacactggc agagcaggcg tcgacgttaa ttaaggcgcg     1020

cc                                                                    1022


<210>  226
<211>  1022
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  226
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagctgttc tagataacaa      960

taaagttggt taattggttg taacactggc agagcaggcg tcgacgttaa ttaaggcgcg     1020

cc                                                                    1022


<210>  227
<211>  1064
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  227
cgcgcgcgtt aattaacctc gaggcctaaa gccacgttgt gtctcaaaat ctctgatgtt       60

acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca      120

gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      180

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      240

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      300

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc      360

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat      420

ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg      480

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc      540

ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac      600

gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg      660

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca      720

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta      780

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact      840

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata      900

atcctgatat gaataaattg cagtttcatt tgatgctcga tgagctgttc ccaagctaaa      960

atttattact caaccgtaac cgattttgcc aggttacgcg gctgtaattg gttaattggt     1020

tgtaacactg gcagagcagg cgtcgacgtt aattaaggcg cgcc                      1064


<210>  228
<211>  1188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  228
cgcgcgcgtt aattaacctc gaggcctcct gcaggaatta agtcttcgaa ccaatacgca       60

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg      120

actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac      180

cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac      240

aatttcacac aggaaacagc tatgagccat attcaacggg aaacgtcttg ctcgaggccg      300

cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc      360

gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt      420

ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac      480

tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat      540

gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat      600

cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg      660

attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa      720

tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg      780

cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc      840

gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt      900

tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg      960

aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt     1020

gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagct gttccaagct     1080

taaaatttat tactcaaccg taaccgattt tgccaggtta cgcggctgta attggttaat     1140

tggttgtaac actggcagag caggcgtcga cgttaattaa ggcgcgcc                  1188


<210>  229
<211>  1188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  229
cgcgcgcgtt aattaacctc gaggcctcct gcaggaatta agtcttcgaa ccaatacgca       60

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg      120

actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac      180

cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac      240

aatttcacac aggaaacagc tatgagccat attcaacggg aaacgtcttg ctcgaggccg      300

cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc      360

gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt      420

ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac      480

tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat      540

gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat      600

cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg      660

attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa      720

tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg      780

cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc      840

gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt      900

tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg      960

aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt     1020

gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagct gttcccaagc     1080

taaaatttat tactcaaccg taaccgattt tgccaggtta cgcggctgta attggttaat     1140

tggttgtaac actggcagag caggcgtcga cgttaattaa ggcgcgcc                  1188


<210>  230
<211>  965
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  230
cgcgcgcgtt aattaacctc gaggcctcca atacgcaaac cgcctctccc cgcgcgttgg       60

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc      120

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt      180

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat      240

ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca      300

ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat      360

tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca      420

cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga      480

gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac      540

gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc      600

gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa      660

tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc      720

caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga      780

caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt      840

cggcagaatg cttaatgaat tacaacagta ctaataaagg gcatccattt attactcaac      900

cgtaaccgat tttgccaggt tacgcggctg ggtgcccggg ccgtcgacgt taattaaggc      960

gcgcc                                                                  965


<210>  231
<211>  965
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  231
cgcgcgcgtt aattaacctc gaggcctcca atacgcaaac cgcctctccc cgcgcgttgg       60

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc      120

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt      180

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat      240

ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca      300

ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat      360

tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca      420

cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga      480

gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac      540

gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc      600

gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa      660

tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc      720

caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga      780

caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt      840

cggcagaatg cttaatgaat tacaacagta ctgctaaagg gcatccattt attactcaac      900

cgtaaccgat tttgccaggt tacgcggctg ggtgcccggg ccgtcgacgt taattaaggc      960

gcgcc                                                                  965


<210>  232
<211>  965
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  232
cgcgcgcgtt aattaacctc gaggcctcca atacgcaaac cgcctctccc cgcgcgttgg       60

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc      120

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt      180

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat      240

ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca      300

ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat      360

tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca      420

cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga      480

gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac      540

gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc      600

gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa      660

tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc      720

caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga      780

caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt      840

cggcagaatg cttaatgaat tacaacagta ctgctaaagg gcatccattt attactcaac      900

cgtaaccgat tttgccaggt tacgcggctg ggtgcccggg ccgtcgacgt taattaaggc      960

gcgcc                                                                  965


<210>  233
<211>  1016
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  233
cgcgcgcgtt aattaacctc gaggcctcca atacgcaaac cgcctctccc cgcgcgttgg       60

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc      120

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt      180

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat      240

gtcttattca aagcaaggca tcgcacaagt aatgaagacg aaataccata tggaaggcag      300

tgtcaatggc catgaattca cgatcgaagg tgtaggaact ggaaaccctt acgaaggcac      360

acagatgtcc gaattagtga tcaccaagcc tgcaggaaaa ccccttccat tctcctttga      420

cattctgtca acagtctttc agtatggaaa caggtgcttc acaaagtacc ctgaaggaat      480

gactgactat ttcaagcaag cattcccaga tggaatgtca tttgaaaggt catttctata      540

tgaggatgga ggagttgcta cagccagctg gaacattcgt cttgagagag attgcttcat      600

ccacaaatcc atctatcatg gcgttaactt tcccgctgat ggacccgtaa tgaaaaagaa      660

gaccattggc tgggataaag ccttcgaaaa aatgactgtg tccaaagacg ttttaagagg      720

tgatgtgact gagtttctta tgctcgaagg aggtggttac cacagctgcc agtttcactc      780

cacttacaaa ccagagaagc cggttacact gcccccgaat catgtcgtgg aacatcacat      840

tgtgaggact gaccttggcc aaactgcaaa aggcttcaca gtcaagctgg aagaacatgc      900

tgcggctcat gttaaccctt tgaaggttca ataaaggatt tattactcaa ccgtaaccga      960

ttttgccagg ttacgcggct gggtgcccgg gccgtcgacg ttaattaagg cgcgcc         1016


<210>  234
<211>  1822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  234
acataacagg aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggcctccaa tacgcaaacc      240

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg      300

gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca      360

ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt      420

tcacacagga aacagctatg accatgatta cgaattcgag ctcggtaccc ggggatcctc      480

tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact      540

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct      600

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg      660

gcgaatggcg cctttaataa aggcgtcgac gttaattaac ctcgaggaaa cggatgaagg      720

cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc      780

gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg      840

ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc      900

aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgctct      960

tctaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg     1020

ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg ggctgctctt     1080

gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca gccggactcc     1140

gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa     1200

gaagcggttg ttggcgctct cgcggcttac gttctgccca ggtttgagca gccgcgtagt     1260

gagatctata tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc     1320

gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg     1380

caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg catacgggaa     1440

gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt tcaagccgag     1500

atcggcttcc cggccgcggc gtcgacgtta attaaggcgc gccagttgtt cggtaaattg     1560

tcacaacgcc gcgaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac     1620

gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg     1680

ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact     1740

ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacaccc     1800

gcttacgcag ggcatccatt ta                                              1822


<210>  235
<211>  1822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  235
acataacagg aagaaaaatg ccccgctgtt ttaattaaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggcctccaa tacgcaaacc      240

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg      300

gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca      360

ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt      420

tcacacagga aacagctatg accatgatta cgaattcgag ctcggtaccc ggggatcctc      480

tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact      540

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct      600

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg      660

gcgaatggcg cctttaataa aggcgtcgac gttaattaac ctcgaggaaa cggatgaagg      720

cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc      780

gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg      840

ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc      900

aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgctct      960

tctaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg     1020

ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg ggctgctctt     1080

gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca gccggactcc     1140

gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa     1200

gaagcggttg ttggcgctct cgcggcttac gttctgccca ggtttgagca gccgcgtagt     1260

gagatctata tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc     1320

gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg     1380

caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg catacgggaa     1440

gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt tcaagccgag     1500

atcggcttcc cggccgcggc gtcgacgtta attaaggcgc gccagttgtt cggtaaattg     1560

tcacaacgcc gcgaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac     1620

gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg     1680

ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact     1740

ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacaccc     1800

gcttacgcag ggcatccatt ta                                              1822


<210>  236
<211>  1822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  236
acataacagg aagaaaaatg ccccgctgtt taattaaaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggcctccaa tacgcaaacc      240

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg      300

gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca      360

ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt      420

tcacacagga aacagctatg accatgatta cgaattcgag ctcggtaccc ggggatcctc      480

tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact      540

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct      600

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg      660

gcgaatggcg cctttaataa aggcgtcgac gttaattaac ctcgaggaaa cggatgaagg      720

cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc      780

gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg      840

ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc      900

aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgctct      960

tctaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg     1020

ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg ggctgctctt     1080

gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca gccggactcc     1140

gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa     1200

gaagcggttg ttggcgctct cgcggcttac gttctgccca ggtttgagca gccgcgtagt     1260

gagatctata tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc     1320

gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg     1380

caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg catacgggaa     1440

gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt tcaagccgag     1500

atcggcttcc cggccgcggc gtcgacgtta attaaggcgc gccagttgtt cggtaaattg     1560

tcacaacgcc gcgaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac     1620

gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg     1680

ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact     1740

ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacaccc     1800

gcttacgcag ggcatccatt ta                                              1822


<210>  237
<211>  1822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  237
acataacagg aagaaaaatg ccccgctgtt ctagataaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggcctccaa tacgcaaacc      240

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg      300

gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca      360

ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt      420

tcacacagga aacagctatg accatgatta cgaattcgag ctcggtaccc ggggatcctc      480

tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact      540

gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct      600

ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg      660

gcgaatggcg cctttaataa aggcgtcgac gttaattaac ctcgaggaaa cggatgaagg      720

cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc      780

gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg      840

ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc      900

aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgctct      960

tctaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg     1020

ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg ggctgctctt     1080

gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca gccggactcc     1140

gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa     1200

gaagcggttg ttggcgctct cgcggcttac gttctgccca ggtttgagca gccgcgtagt     1260

gagatctata tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc     1320

gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg     1380

caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg catacgggaa     1440

gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt tcaagccgag     1500

atcggcttcc cggccgcggc gtcgacgtta attaaggcgc gccagttgtt cggtaaattg     1560

tcacaacgcc gcgaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac     1620

gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg     1680

ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact     1740

ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacaccc     1800

gcttacgcag ggcatccatt ta                                              1822


<210>  238
<211>  1868
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial Sequence

<400>  238
acataacagg aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggccttggc gggatcgttg      240

tatatttctt gacacctttt cggcatcgcc ctaaaattcg gcgtcctcat attgtgtgag      300

gacgttttat tacgtgttta cgaagcaaaa gctaaaacca ggagctattt aatggcaaca      360

gttaaccagc tggtacgcaa accacgtgct cgcaaagttg cgaaaagcaa cgtgcctgcg      420

ctggaagcat gcccgcaaaa acgtggcgta tgtactcgtg tatatactac cactcctaaa      480

aaaccgaact ccgcgctgcg taaagtatgc cgtgttcgtc tgactaacgg tttcgaagtg      540

acttcctaca tcggtggtga aggtcacaac ctgcaggagc actccgtgat cctgatccgt      600

ggcggtcgtg ttaaagacct cccgggtgtt cgttaccaca ccgtacgtgg tgcgcttgac      660

tgctccggcg ttaaagaccg taagcaggct cgttccaagt atggcgtgaa gcgtcctaag      720

gcttaaaggc gtcgacgtta attaacctcg aggaaacgga tgaaggcacg aacccagttg      780

acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg      840

tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt      900

tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc      960

gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgctcttcta acgatgttac     1020

gcagcagggc agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac     1080

atgtaggctc ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg     1140

tgagttcgga gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa     1200

cttgctccgt agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg     1260

cgctctcgcg gcttacgttc tgcccaggtt tgagcagccg cgtagtgaga tctatatcta     1320

tgatctcgca gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct     1380

cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg     1440

tgacgatccc gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt     1500

tgatatcgac ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc     1560

cgcggcgtcg acgttaatta aggcgcgcca gttgttcggt aaattgtcac aacgccgcga     1620

atatagtctt taccatgccc ttggccacgc ccctctttaa tacgacgggc aatttgcact     1680

tcagaaaatg aagagtttgc tttagccata acaaaagtcc agtatgcttt ttcacagcat     1740

aactggactg atttcagttt acaactattc tgtctagttt aagactttat tgtcatagtt     1800

tagatctatt ttgttcagtt taagacttta ttgtccgccc acacccgctt acgcagggca     1860

tccattta                                                              1868


<210>  239
<211>  2278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic DNA

<400>  239
acataacagg aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actgggaggg       60

gtggaaatgg agtttttaag gattatttag ggaagagtga caaaatagat gggaactggg      120

tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc      180

acttatctgg ttcggatctc gcgcgcgtta attaacctcg aggcctccaa tacgcaaacc      240

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg      300

gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca      360

ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt      420

tcacacagga aacagctatg tcttattcaa agcaaggcat cgcacaagta atgaagacga      480

aataccatat ggaaggcagt gtcaatggcc atgaattcac gatcgaaggt gtaggaactg      540

gaaaccctta cgaaggcaca cagatgtccg aattagtgat caccaagcct gcaggaaaac      600

cccttccatt ctcctttgac attctgtcaa cagtctttca gtatggaaac aggtgcttca      660

caaagtaccc tgaaggaatg actgactatt tcaagcaagc attcccagat ggaatgtcat      720

ttgaaaggtc atttctatat gaggatggag gagttgctac agccagctgg aacattcgtc      780

ttgagagaga ttgcttcatc cacaaatcca tctatcatgg cgttaacttt cccgctgatg      840

gacccgtaat gaaaaagaag accattggct gggataaagc cttcgaaaaa atgactgtgt      900

ccaaagacgt tttaagaggt gatgtgactg agtttcttat gctcgaagga ggtggttacc      960

acagctgcca gtttcactcc acttacaaac cagagaagcc ggttacactg cccccgaatc     1020

atgtcgtgga acatcacatt gtgaggactg accttggcca aactgcaaaa ggcttcacag     1080

tcaagctgga agaacatgct gcggctcatg ttaacccttt gaaggttcaa taataaaggc     1140

gtcgacgtta attaacctcg aggaaacgga tgaaggcacg aacccagttg acataagcct     1200

gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct     1260

tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt     1320

tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat     1380

gtttgatgtt atggagcagc aacgatgtta cgctcttcta acgatgttac gcagcagggc     1440

agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc     1500

ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga     1560

gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt     1620

agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg     1680

gcttacgttc tgcccaggtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca     1740

gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat     1800

gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc     1860

gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac     1920

ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggcgtcg     1980

acgttaatta aggcgcgcca gttgttcggt aaattgtcac aacgccgcga atatagtctt     2040

taccatgccc ttggccacgc ccctctttaa tacgacgggc aatttgcact tcagaaaatg     2100

aagagtttgc tttagccata acaaaagtcc agtatgcttt ttcacagcat aactggactg     2160

atttcagttt acaactattc tgtctagttt aagactttat tgtcatagtt tagatctatt     2220

ttgttcagtt taagacttta ttgtccgccc acacccgctt acgcagggca tccattta       2278


<210>  240
<211>  240
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence


<220>
<221>  misc_feature
<222>  (1)..(193)
<223>  Partial sequence of cat gene before junction with 5' end of Tn7L

<220>
<221>  Unsure
<222>  (192)..(192)

<220>
<221>  misc_feature
<222>  (194)..(240)
<223>  5' end of Tn7L

<220>
<221>  Unsure
<222>  (194)..(194)

<220>
<221>  Unsure
<222>  (197)..(197)

<220>
<221>  Unsure
<222>  (199)..(201)

<220>
<221>  Unsure
<222>  (203)..(203)

<400>  240
caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt       60

cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct      120

ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga      180

attacaacag tncngtngnn ngncaaaata gttgggaact gggaggggtg gaaatggagt      240


