                         SEQUENCE LISTING

<110>  Cellecta, Inc.
       Chenchik, Alex
       Darst IV, Russell P.
       Kobzik, Lester
       Makhanov, Mikhail
       Tedesco, Donato
 
<120>  COMPARTMENT-FREE SINGLE CELL GENETIC ANALYSIS

<130>  CLCT-007WO

<140>  PCT/US20/45865
<141>  2020-08-12

<150>  US 62/895,719
<151>  2019-09-04

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  1
agcaccgacc agcagaca                                                     18


<210>  2
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  2
agcaccgacc agcacaga                                                     18


<210>  3
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  3
aatgatacgg cgaccaccga                                                   20


<210>  4
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  4
caagcagaag acggcatacg agat                                              24


<210>  5
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  5
acactctttc cctacacgac gctcttccga tct                                    33


<210>  6
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  6
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  7
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  7
ccatctcatc cctgcgtgtc tccgactcag                                        30


<210>  8
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  8
cctctctatg ggcagtcggt gat                                               23


<210>  9
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to a phosphate

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  The nucleotide is attached to RevGSP

<400>  9
ccacgaccag cca                                                          13


<210>  10
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to X-Bead

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  A barcode is present between the nucleotides

<400>  10
acgagcaccg accagcacag aaccgcacga ccg                                    33


<210>  11
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  11
acgagcaccg accagcacag agaacaaaca ccgcacgacc g                           41


<210>  12
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  12
acgagcaccg accagcacag aggcgaaaca ccgcacgacc g                           41


<210>  13
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  13
acgagcaccg accagcacag agcaaaagga ccgcacgacc g                           41


<210>  14
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to a PClinker which is attached to a 
       linker which is attached to a bead

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<400>  14
agcaccgacc agcacagavv nvvnvvcatc agaccgcacg accg                        44


<210>  15
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to an SSlinker which is attached to a 
       linker which is attached to a bead

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<400>  15
agcaccgacc agcacagavv nvvnvvcagc atgaccgcac gaccg                       45


<210>  16
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  UMI-barcode is present between the nucleotides

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  The nucleotide is attached to RevGSP

<400>  16
acgagcaccg accagcacag aaccgcacga ccgccacgac cagcca                      46


<210>  17
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  UMI-Barcode is present between the nucleotides

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  The nucleotide is attached to dT30VN

<400>  17
acgagcaccg accagcacag aaccgcacga ccgccacgac cagcca                      46


<210>  18
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  UMI-Barcode is present between the nucleotides

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  The nucleotide is attached to RevGSP

<400>  18
agcaccgacc agcagacaac cgcacgaccg ccacgaccag cca                         43


<210>  19
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  The nucleotide is attached to FwdGSP

<400>  19
agcaccgacc agcagaca                                                     18


<210>  20
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  The nucleotide is attached to a moiety

<400>  20
ccacgaccag cca                                                          13


<210>  21
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  21
ccaccaccgt acaattcgct ttcttttttc attacctact ctggc                       45


<210>  22
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to a phosphate

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  The nucleotide is attached to NH2

<400>  22
ccacgaccag cca                                                          13


<210>  23
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to NH2

<400>  23
tggctggtcg tggcggtcgt gcggt                                             25


<210>  24
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  24
agcagcaccg accagcagac                                                   20


<210>  25
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  The nucleotide may be attached to (BC-Link) FwdGSP

<400>  25
agcaccgacc agcagaca                                                     18


<210>  26
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to (Link-BC) which is attached to 
       RevGSP

<400>  26
agacacgacc agccacga                                                     18


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  27
gacacgacca gccacgagca                                                   20


<210>  28
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  FwdGSP-cDNA-RevGSP-Link-BC is present between the nucleotides

<400>  28
agcagcaccg accagcagac atctgtgctg gtcggtgctc gt                          42


<210>  29
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  FwdGSP-cDNA-RevGSP-Link-BC is present between the nucleotides

<400>  29
tcgtcgtggc tggtcgtctg tagacacgac cagccacgag ca                          42


<210>  30
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  30
agcagaagac ggcatacgag atatacgaca gcagcagcac cgaccagcag aca              53


<210>  31
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  31
agcagaagac ggcatacgag atactgatga gcagcagcac cgaccagcag aca              53


<210>  32
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  32
agcagaagac ggcatacgag atagcatcaa gcagcagcac cgaccagcag aca              53


<210>  33
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  33
agcagaagac ggcatacgag ataagtcgta gcagcagcac cgaccagcag aca              53


<210>  34
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  34
agcagaagac ggcatacgag atatcgcata gcagcagcac cgaccagcag aca              53


<210>  35
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  35
agcagaagac ggcatacgag atacatagca gcagcagcac cgaccagcag aca              53


<210>  36
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  36
acggcgacca ccgagatcta cacatacgac acgacgagca ccgaccagca caga             54


<210>  37
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  37
acggcgacca ccgagatcta cacactgatg acgacgagca ccgaccagca caga             54


<210>  38
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  38
acggcgacca ccgagatcta cacagcatca acgacgagca ccgaccagca caga             54


<210>  39
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  39
acggcgacca ccgagatcta cacaagtcgt acgacgagca ccgaccagca caga             54


<210>  40
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  40
acggcgacca ccgagatcta cacatcgcat acgacgagca ccgaccagca caga             54


<210>  41
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  41
acggcgacca ccgagatcta cacacatagc acgacgagca ccgaccagca caga             54


<210>  42
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  42
agcagaagac ggcatacgag atagcagcag caccgaccag cagaca                      46


<210>  43
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  43
acggcgacca ccgagatcta cacacgacga gcaccgacca gcacaga                     47


<210>  44
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to (Ind) which is attached to p7

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  FwdGSP-cDNA-RevGSP(LinkBC) is present between the nucleotides

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  The nucleotide is attached to (Ind)p5

<400>  44
agcagcaccg accagcagac atctgtgctg gtcggtgctc gt                          42


<210>  45
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to (Ind) which is attached to p7

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  FwdGSP-cDNA-RevGSP(LinkBC) is present between the nucleotides

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  The nucleotide is attached to (Ind)p5

<400>  45
tcgtcgtggc tggtcgtctg tagacacgac cagccacgag ca                          42


<210>  46
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  46
tggcgtgctg gcggtgctgg tcggt                                             25


<210>  47
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  47
agcagcagca ccgaccagca gaca                                              24


<210>  48
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  48
accgaccagc accgccagca cgcca                                             25


<210>  49
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  49
tctgtgctgg tcggtgctcg tcgt                                              24


<210>  50
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  50
tgtctgctgg tcggtgctgc tgct                                              24


<210>  51
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence

<400>  51
acgacgagca ccgaccagca caga                                              24


<210>  52
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  The nucleotide is attached to deoxythymine

<400>  52
atggctggtc gtggcggtcg tgcggt                                            26


<210>  53
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  The nucleotide is attached to FwdGSP

<400>  53
agcaccgacc agcagaca                                                     18


<210>  54
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The nucleotide is attached to (BC-Link) which is attached to 
       RevGSP

<400>  54
agacacgacc agccacga                                                     18


